Archives of Virology最新文献_第6页

Complete genome analysis of a novel nyamivirus from the leaf beetle Aulacophora lewisii

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-13 DOI: 10.1007/s00705-025-06246-0

Meng-Nan Chen, Xin-Yi Cai, Zhuang-Xin Ye, Ke-Hui Feng, Peng-Peng Ren, Chuan-Xi Zhang, Jian-Ping Chen, Jun-Min Li, Qianzhuo Mao

{"title":"Complete genome analysis of a novel nyamivirus from the leaf beetle Aulacophora lewisii","authors":"Meng-Nan Chen, Xin-Yi Cai, Zhuang-Xin Ye, Ke-Hui Feng, Peng-Peng Ren, Chuan-Xi Zhang, Jian-Ping Chen, Jun-Min Li, Qianzhuo Mao","doi":"10.1007/s00705-025-06246-0","DOIUrl":"10.1007/s00705-025-06246-0","url":null,"abstract":"<div><p>Leaf beetles of the subfamily Chrysomelinae are not only herbivorous pests but also potential vectors of plant pathogens. Here, we describe a novel negative-sense virus named \"Aulacophora lewisii nyamivirus 1\" (ALNyV1), discovered in a leaf beetle (<i>Aulacophora lewisii</i>), a prevalent pest of cucurbitaceous vegetables. Using transcriptome sequencing and rapid amplification of cDNA ends (RACE) techniques, the complete genome sequence of ALNyV1, spanning 10,339 nucleotides (excluding the polyA tail), was determined. A homology search and phylogenetic analysis indicated that ALNyV1 belongs to an unclassified clade in the family <i>Nyamiviridae</i>. This virus contains five open reading frames (ORFs) with typical conserved domains of nyamiviruses. The mean coverage of the ALNyV1 genome was 249, suggesting that active replication had occurred within the leaf beetle host. Furthermore, the virus was found to trigger a small-RNA interference response, with virus-derived small RNAs (vsiRNA) of ALNyV1 displaying a distinct pattern with 21 nucleotides being the most frequent length and exhibiting an A/U bias at the 5' end. This is the first detection of a negative-sense RNA virus in this leaf beetle species, providing important new information about the characteristics of viruses found in leaf beetles.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of a novel narnavirus from the plant-pathogenic fungus Ustilaginoidea virens

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-13 DOI: 10.1007/s00705-025-06241-5

Wenhua Zhao, Zhenrui He, Sisi Liao, Mei Yang, Yingqing Yang, Baoping Cheng, Erxun Zhou

{"title":"Molecular characterization of a novel narnavirus from the plant-pathogenic fungus Ustilaginoidea virens","authors":"Wenhua Zhao, Zhenrui He, Sisi Liao, Mei Yang, Yingqing Yang, Baoping Cheng, Erxun Zhou","doi":"10.1007/s00705-025-06241-5","DOIUrl":"10.1007/s00705-025-06241-5","url":null,"abstract":"<div><p>Mycoviruses are viruses that infect fungi, yeasts, and oomycetes and can replicate and multiply within them. They are widely distributed in plant- and animal-pathogenic fungi. In this study, we identified a novel positive-sense single-stranded RNA (+ ssRNA) mycovirus from <i>Ustilaginoidea virens</i> strain Uv339, the causal agent of rice false smut (RSF), and this virus was named \"Ustilaginoidea virens narnavirus 5\" (UvNV5). Sequence analysis revealed that UvNV5 has a complete genome length of 2091 nt and contains a single open reading frame (ORF) (nt 131–2038) encoding a 635-amino-acid (aa) RNA-dependent RNA polymerase (RdRp) with a molecular mass of 71.8 kDa. BLASTp analysis revealed that the RdRp of UvNV5 shares only 38.22% amino acid sequence identity with that of Ustilaginoidea virens narnavirus virus 13, its closest relative. Phylogenetic analysis indicated that UvNV5 clustered within a branch alongside members of the class <i>Amabiliviricetes</i>. In summary, UvNV5 is a novel mycovirus of the class <i>Amabiliviricetes</i> that infects <i>U. virens</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel SIV-infection animal model based on nude mice inoculated with MT-2 cells

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-13 DOI: 10.1007/s00705-025-06247-z

Lei Xie, Junlin Li, Xiuyun Chen, Mingyu Yin, Wenguan Huang, Minyi Luo, Yatian Liu, Haolan He, Xinyu Zhang, Jinyang He

{"title":"A novel SIV-infection animal model based on nude mice inoculated with MT-2 cells","authors":"Lei Xie, Junlin Li, Xiuyun Chen, Mingyu Yin, Wenguan Huang, Minyi Luo, Yatian Liu, Haolan He, Xinyu Zhang, Jinyang He","doi":"10.1007/s00705-025-06247-z","DOIUrl":"10.1007/s00705-025-06247-z","url":null,"abstract":"<div><p>To establish a novel murine model conducive to simian immunodeficiency virus (SIV) replication <i>in vivo</i>, male BALB/c nude mice, aged three weeks, were administered a single intraperitoneal inoculation of SIV-infected MT-2 cells (derived from human T-cell leukemia). Subsequently, alterations in plasma viral load and the engraftment of MT-2 cells <i>in vivo</i> were examined, while immunological changes were assessed via ELISA and flow cytometry analysis. All of the mice in the experimental group exhibited detectable plasma viral loads in their peripheral circulation, largely due to the proliferation of SIV-infected MT-2 cells within the T-cell-deficient environment of nude mice, resulting in colonization of the abdominal cavity and lymph nodes and the subsequent release of free virions to perpetuate the infection. Mice that received highly active anti-retroviral therapy (HAART) treatment demonstrated a statistically significant reduction in plasma viral loads, with HAART administration partially reversing the course of SIV-induced immune dysfunction. Therefore, the model presented in this study offers substantial potential as a robust tool for evaluating antiviral efficacy and immune modulation in an <i>in vivo</i> setting.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of the novel Vibrio parahaemolyticus phage vB_VpaM_PVP-XSN 新型副溶血性弧菌噬菌体 vB_VpaM_PVP-XSN 的分离和特征描述

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-11 DOI: 10.1007/s00705-025-06237-1

Tianpeng Qi, Sunjian Lyu, Meiwen Xia, Bao Lou, Li Liu, Haisheng Xu

引用次数: 0

Tahyna virus: an emerging threat to public health

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-10 DOI: 10.1007/s00705-024-06217-x

Wenbing Zhu, Lijun Shao, Shuo Feng, Ke Lu, Xiaoli Yu, Guoyu Niu

{"title":"Tahyna virus: an emerging threat to public health","authors":"Wenbing Zhu, Lijun Shao, Shuo Feng, Ke Lu, Xiaoli Yu, Guoyu Niu","doi":"10.1007/s00705-024-06217-x","DOIUrl":"10.1007/s00705-024-06217-x","url":null,"abstract":"<div><p>Tahyna virus (TAHV), a member of the California serogroup of the genus <i>Orthobunyavirus</i>, is an arbovirus that is capable of causing a range of symptoms from mild febrile illnesses to severe neuroinvasive disease. It was first isolated in Europe and has a holarctic distribution, spanning Central Europe, Asia, and Africa. Central Europe has reported the highest reported human seropositivity rate, reaching 30%. Phylogenetic analysis has revealed a close relationship between TAHV and Lumbo virus (LUMV), with 94% amino acid sequence identity in their N proteins. TAHV is mainly transmitted by <i>Aedes</i> mosquitoes, and its vertebrate hosts include hares, rabbits, hedgehogs, and rodents. Flooding during the summer months due to climate change may be a catalyst for the spread of the virus. Despite the enormous potential threat of TAHV and its potential to cause meningitis, there is a notable dearth of information regarding its actual prevalence, the number of symptomatic cases, and its clinical manifestations in humans and animals. To date, no deaths have been attributed to TAHV, which might contribute to an underestimation of its public health significance. Given the anticipated future changes in climatic conditions, TAHV could pose a health threat in Europe and elsewhere. In this review, we summarize recent advancements in TAHV research, highlighting the need for enhanced research and resource allocation for its prevention and control.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and genomic analysis of Cronobacter sakazakii phage JC03, a new member of the genus Pseudotevenvirus

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-10 DOI: 10.1007/s00705-025-06245-1

Jie Jiang, Jingying Sai, Xin Huang, Lingling Sun, Jinghua Li, Honglan Huang, Yanbo Sun, Chunyan Zhao

引用次数: 0

Intraspecific variation of the hedgehog arteriviruses, which may constitute a new genus in the subfamily Heroarterivirinae of the family Arteriviridae

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-08 DOI: 10.1007/s00705-025-06231-7

Akbar Dastjerdi, Hannah Davies, Nadia Inglese, Samantha Holland, Dmitry V. Samborskiy, Alexander E. Gorbalenya

{"title":"Intraspecific variation of the hedgehog arteriviruses, which may constitute a new genus in the subfamily Heroarterivirinae of the family Arteriviridae","authors":"Akbar Dastjerdi, Hannah Davies, Nadia Inglese, Samantha Holland, Dmitry V. Samborskiy, Alexander E. Gorbalenya","doi":"10.1007/s00705-025-06231-7","DOIUrl":"10.1007/s00705-025-06231-7","url":null,"abstract":"<div><p>We recently discovered a novel member of the family <i>Arteriviridae</i>, hedgehog arterivirus 1 (HhAV-1), in the brains of hedgehogs with fatal encephalitis. In this study, we classified this virus and investigated its intrahost genomic diversity using next-generation sequencing. We sequenced HhAV-1 genomes from specimens from seven hedgehogs (two males and five females) with signs of encephalitis that were collected in Buckinghamshire, Gloucestershire, and Cambridgeshire, England, and had died or been euthanised between 2013 and 2024. Analysis of the intrahost populations of these seven HhAV-1 isolates and a previously described isolate revealed the presence of single-nucleotide polymorphisms (SNPs), which were most frequent in open reading frames 5, 6, and 7, encoding glycoprotein 5, the membrane protein, and the nucleocapsid protein. Pairwise comparisons of the eight HhAV-1 variants showed that the nucleotide sequence identity values in their combined complete coding sequences ranged from 76.2% to 100%. The eight HhAV-1 variants also shared at least 82.8% amino acid sequence identity in five domains that are involved in replication and are used for the classification of nidoviruses: 3CLpro, NiRAN, RdRp, ZBD, and HEL1. In a replicase-based phylogenetic tree of members of the family <i>Arteriviridae</i>, the HhAV-1 variants formed a sister cluster to African pouched rat arterivirus. A DEmARC-based pairwise distance analysis indicated that these viruses may comprise a new species, for which we propose the name \"<i>Xiarterivirus erinaceid</i>\", in a new genus in the subfamily <i>Heroarterivirinae</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-025-06231-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143361837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of a novel deltaflexivirus from the plant-pathogenic fungus Neopestalotiopsis nebuloides strain N-7

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-08 DOI: 10.1007/s00705-025-06219-3

Caiming Wu, Siyu Zhou, Changping Xie, Daipeng Chen, Li Zheng

引用次数: 0

Dengue: epidemiology, diagnosis methods, treatment options, and prevention strategies

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-06 DOI: 10.1007/s00705-025-06235-3

Dimple Kothari, Niralee Patel, Ashok Kumar Bishoyi

{"title":"Dengue: epidemiology, diagnosis methods, treatment options, and prevention strategies","authors":"Dimple Kothari, Niralee Patel, Ashok Kumar Bishoyi","doi":"10.1007/s00705-025-06235-3","DOIUrl":"10.1007/s00705-025-06235-3","url":null,"abstract":"<div><p>Dengue is an arboviral disease caused by dengue virus, which is mostly found in tropical regions, and the number of human cases has increased dramatically since 2000, with 5.2 million cases reported in 2019, according to WHO reports, 70% of which were in Southeast Asia, the Western Pacific, and Asia. Dengue infection can result in a wide range of clinical manifestations, ranging from fever to severe dengue shock syndrome, which can be fatal, particularly in those with secondary dengue. This review of the aetiology of dengue fever examines the complex interactions between the virus and the immune system and the interaction between viral and host factors and also covers outbreaks, the severity of disease caused by different serotypes, and methods for diagnosis of dengue, such as serological tests, nucleic acid amplification tests, and ELISA assays for detecting the NS1 antigen. Current treatment options and prevention strategies, including vector control measures, environmental interventions, and insect repellents are also discussed. This review highlights the challenges involved in developing a dengue vaccine, which is complicated by the need for an efficient and balanced immune response against all genotypes of the four serotypes.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143361941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, characterization, and whole-genome analysis of the novel temperate bacteriophage Ph-p5 infecting Glutamicibacter halophytocola

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-05 DOI: 10.1007/s00705-025-06225-5

Viktoryia Savich, Artur Akhremchuk, Aliaksandra Herasimovich, Sviatlana Leanovich, Leonid Valentovich, Anastasiya Sidarenka

{"title":"Isolation, characterization, and whole-genome analysis of the novel temperate bacteriophage Ph-p5 infecting Glutamicibacter halophytocola","authors":"Viktoryia Savich, Artur Akhremchuk, Aliaksandra Herasimovich, Sviatlana Leanovich, Leonid Valentovich, Anastasiya Sidarenka","doi":"10.1007/s00705-025-06225-5","DOIUrl":"10.1007/s00705-025-06225-5","url":null,"abstract":"<div><p>Bacteria of the genus <i>Glutamicibacter</i>, due to their ubiquity, nutritional versatility, and ability to adapt to environmental stresses, play an important role in natural ecosystems and have been extensively studied. Nevertheless, there remains a significant gap in our knowledge regarding <i>Glutamicibacter</i> phages, particularly in comparison to those of the related actinobacteria. To date, only two virulent phages infecting <i>G. arilaitensis</i> have been described. To our knowledge, this is the first report on the isolation, characterization, and genome analysis of a temperate phage targeting <i>G. halophytocola</i>, an endophytic bacterium known for its plant-growth-promoting effect. The phage Ph-p5, with siphovirus structure, was isolated from soil. It exhibited a long latent period (120 min), a moderate burst size (87 ± 3 PFU/infected cell), and stability over a wide range of pH and temperature. The circularly permuted linear double-stranded DNA genome of phage Ph-p5, comprising 43,694 bp with a G + C content of 57.1%, contains 65 putative protein-coding sequences and one sequence encoding tRNA. Of the identified open reading frames, 33 were of unknown function, while the remaining ones were grouped into functional modules, including structural proteins, DNA replication and regulation, lysogeny, and lysis. The presence of intact lysogeny-related genes, together with the capacity for lysogenisation of the host strain <i>G. halophytocola</i> BIM B-1594, provides evidence that Ph-p5 is a temperate phage. Phylogenetic analysis demonstrated that phage Ph-p5 belongs to the class <i>Caudoviricetes</i> but exhibits significant divergence from known phages and may be assigned to a new genus, for which we propose the name “<i>Petuglutavirus”</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143184785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0