Archives of Virology最新文献_第6页

Coxsackievirus B regulates host circAKAP10 expression to promote viral replication 柯萨奇病毒B通过调控宿主circAKAP10表达促进病毒复制。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-27 DOI: 10.1007/s00705-025-06348-9

Yuhan Li, Hua Wang, Fangqian Liu, Xiaolan Liu, Mei Zhang, Zengjun Ji, Hongxing Shen

{"title":"Coxsackievirus B regulates host circAKAP10 expression to promote viral replication","authors":"Yuhan Li, Hua Wang, Fangqian Liu, Xiaolan Liu, Mei Zhang, Zengjun Ji, Hongxing Shen","doi":"10.1007/s00705-025-06348-9","DOIUrl":"10.1007/s00705-025-06348-9","url":null,"abstract":"<div>\u0000 \u0000 <p>Coxsackievirus B (CVB) infection is associated with severe clinical manifestations including myocarditis and encephalitis. However, the molecular mechanisms underlying viral replication remain unclear. The role of circular RNAs (circRNAs), which are known to regulate cellular processes by acting as miRNA sponges or interacting with proteins, has not been thoroughly explored in CVB pathogenesis. Here, we identified hsa_circ_0042409 (circAKAP10) as a proviral circRNA that is significantly upregulated in CVB-infected HeLa cells. Functional studies demonstrated that circAKAP10 overexpression enhanced viral replication and virion production, whereas its silencing suppressed CVB titers. Mechanistically, circAKAP10 sequesters miR-1256, which in turn derepresses the <i>NTRK2</i> gene, which encodes a kinase that is critical for activation of ERK signaling. Dual-luciferase assays confirmed direct binding between circAKAP10 and miR-1256, as well as between miR-1256 and the 3'UTR of <i>NTRK2</i>. Inhibiting NTRK2 or ERK phosphorylation blocked the pro-replicative effects of circAKAP10. These findings delineate a novel regulatory axis (circAKAP10/miR-1256/NTRK2/ERK) that promotes CVB replication and provides a potential therapeutic target for treating enteroviral infections.</p>\u0000 </div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2024 taxonomy update for the family Retroviridae 2024年逆转录病毒科分类更新。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-27 DOI: 10.1007/s00705-025-06353-y

Jens Mayer, Daniel Blanco-Melo, John M. Coffin, Robert J. Gifford, Welkin E. Johnson, Dirk Lindemann, Martine Peeters, Kei Sato, Jonathan Stoye, Gilda Tachedjian, Theodora Hatziioannou

引用次数: 0

Appearance and spread of norovirus genotype GII.17 subcluster C2 (Romania-2021 like) in Nizhny Novgorod, Russia, 2021–2023 2021-2023年俄罗斯下诺夫哥罗德市诺如病毒基因型GII.17 C2亚群（罗马尼亚-2021样）的出现和传播

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-27 DOI: 10.1007/s00705-025-06356-9

Natalia V. Epifanova, S. V. Oparina, O. V. Morozova, T. A. Sashina, A. E. Alekseeva, N. A. Novikova

{"title":"Appearance and spread of norovirus genotype GII.17 subcluster C2 (Romania-2021 like) in Nizhny Novgorod, Russia, 2021–2023","authors":"Natalia V. Epifanova, S. V. Oparina, O. V. Morozova, T. A. Sashina, A. E. Alekseeva, N. A. Novikova","doi":"10.1007/s00705-025-06356-9","DOIUrl":"10.1007/s00705-025-06356-9","url":null,"abstract":"<div>\u0000 \u0000 <p>Noroviruses are one of the leading causes of acute gastroenteritis worldwide and have significant genetic diversity. In this study, based on phylogenetic analysis of genome sequences of noroviruses circulating in Nizhny Novgorod in 2014–2023 and previously published sequences obtained from the GenBank database, we show a return to active circulation of cluster C of genotype GII.17[P17], which was displaced in 2015–2016 by cluster D. A new subcluster, C2 (Romania-2021 like), was identified and found to be different from subcluster C1 (Kawasaki-2014), which circulated in the middle of the last decade. Amino acid substitutions characteristic of C2 were found in the main structural protein VP1, bringing it closer to the Tokyo_JP_1976 strain identified in the 1970s. It was established that representatives of the C2 subcluster (Romania-2021 like) circulated in 2021–2023 in European and American countries and caused outbreaks of norovirus infection. The data obtained indicate that the evolution of the phylogenetic lineage represented by cluster C of the GII.17 genotype continued in the last decade and had the character of a return to the ancestral strain. Moreover, these processes were not detected for four years (2017–2020).</p>\u0000 </div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production and immunogenicity of a plant-produced beak and feather disease virus vaccine in Japanese quails 日本鹌鹑植物产喙羽病病毒疫苗的制备及其免疫原性。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-25 DOI: 10.1007/s00705-025-06352-z

Goodman Mulondo, Mélie L. R. Buyse, Kimberley Labuschagne, David Jarvis, Albertha van Zyl, Edward P. Rybicki, Inga I. Hitzeroth, Sandiswa Mbewana

{"title":"Production and immunogenicity of a plant-produced beak and feather disease virus vaccine in Japanese quails","authors":"Goodman Mulondo, Mélie L. R. Buyse, Kimberley Labuschagne, David Jarvis, Albertha van Zyl, Edward P. Rybicki, Inga I. Hitzeroth, Sandiswa Mbewana","doi":"10.1007/s00705-025-06352-z","DOIUrl":"10.1007/s00705-025-06352-z","url":null,"abstract":"<div><p>Beak and feather disease virus (BFDV), a single-stranded DNA virus, infects endangered psittacine species, including the South African Cape parrot. The disease is highly contagious and can be transmitted through contact with contaminated faeces, crop secretions, and feather and skin dander. To date, there is no vaccine or cure available for BFDV. The production of an effective vaccine depends on having a production platform and methods that are both easy to use and capable of yielding a significant amount of protein that will induce a sufficient immune response. Therefore, the aim of this study was to produce a plant-based BFDV vaccine candidate and to evaluate its ability to elicit an immune response in birds.</p><p>Recombinant BFDV capsid protein (CP) was transiently expressed in <i>Nicotiana benthamiana</i> and purified using density gradient ultracentrifugation. Japanese quails were immunized with purified BFDV CP. Yolk-derived IgY was purified by water dilution and salt precipitation, and its specificity was verified by western blot analysis. The expression levels of the coat protein increased from non-detectable to an average accumulation of 1.58 mg/kg of fresh plant tissue biomass, and antibodies against BFDV CP were detected in both the blood and eggs of immunized quails, indicating that vaccination with BFDV CP successfully elicited a humoral immune response.</p><p>This study demonstrates that heterologous expression in plants is a viable method for producing BFDV CP. To the best of our knowledge, this is the first study to show the antibody response to a plant-produced BFDV antigen in a quail model. Given that the presence of anti-CP antibodies in infected birds is associated with immunity, this system can potentially be used to produce a vaccine against BFDV.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental variability and pathogen synergy influencing tilapia lake virus outbreaks on tilapia farms in Tamil Nadu, India 影响印度泰米尔纳德邦罗非鱼养殖场罗非鱼湖病毒爆发的环境变异性和病原体协同作用。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-24 DOI: 10.1007/s00705-025-06355-w

Alagukanthasami Ponsrinivasan, Arumugam Uma, P. Chidambaram, Cheryl Antony, Arun Sudhagar, Sethu Selvaraj, Subash Palaniappan, Manimozhi Ethirajan

{"title":"Environmental variability and pathogen synergy influencing tilapia lake virus outbreaks on tilapia farms in Tamil Nadu, India","authors":"Alagukanthasami Ponsrinivasan, Arumugam Uma, P. Chidambaram, Cheryl Antony, Arun Sudhagar, Sethu Selvaraj, Subash Palaniappan, Manimozhi Ethirajan","doi":"10.1007/s00705-025-06355-w","DOIUrl":"10.1007/s00705-025-06355-w","url":null,"abstract":"<div>\u0000 \u0000 <p>Tilapia lake virus (TiLV) has emerged as one of the important viral pathogens affecting wild and farmed tilapia worldwide. In this study, we investigated the prevalence of TiLV and its association with coinfections and environmental parameters (pH, temperature, ammonia, and nitrite) on tilapia farms around Tamil Nadu, India. Principal component analysis (PCA) was used to analyse the associations between variables by assigning severity scores (0, 1, 2). The PCA results identified lower temperatures during the northeast monsoon as a key driver of TiLV outbreaks, along with coinfections with bacteria such as <i>Aeromonas veronii</i>, <i>Streptococcus agalactiae</i>, and <i>Enterococcus</i> sp., which exacerbate disease severity. Histopathological examination revealed prominent syncytial giant cells and tissue necrosis in TiLV-infected fish. The outbreaks were most frequent at water temperatures around 20–21°C, coinciding with sudden rainfall and temperature drops in the infected regions. These findings emphasize the importance of understanding environmental factors and coinfections to improve disease management strategies and mitigate the impact of TiLV on tilapia farming in Tamil Nadu, India.</p>\u0000 </div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and complete genome sequence of a new cheravirus discovered in grapevine 葡萄中一种新冠状病毒的鉴定及全基因组序列分析。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-21 DOI: 10.1007/s00705-025-06340-3

Xudong Fan, Yuxin Hao, Fang Ren, Guojun Hu, Ying Zhang, Qingyun Yuan, Tingting Du, Jie Gao, Meilong Xu, Yafeng Dong

{"title":"Identification and complete genome sequence of a new cheravirus discovered in grapevine","authors":"Xudong Fan, Yuxin Hao, Fang Ren, Guojun Hu, Ying Zhang, Qingyun Yuan, Tingting Du, Jie Gao, Meilong Xu, Yafeng Dong","doi":"10.1007/s00705-025-06340-3","DOIUrl":"10.1007/s00705-025-06340-3","url":null,"abstract":"<div><p>High-throughput sequencing (HTS) was applied to identify viruses present in grapevine samples collected in Ningxia, China, that exhibited obvious symptoms of viral disease. A putative novel cheravirus was found through the analysis of sequencing data, and its presence was confirmed by RT-PCR in a Cabernet Sauvignon sample with vein yellowing. The complete genome sequence of this virus was determined by Sanger sequencing of overlapping RT-PCR products and the rapid amplification of cDNA ends (RACE) method. The genome of the putative virus comprises two RNA segments 7,395 and 3,645 nucleotides in length, excluding their poly-A tails. The new virus was tentatively named “grapevine cheravirus” (GCheV). The Pro-Pol region of RNA1 and the CP region of RNA2 of GCheV shared the highest amino acid sequence identity, reaching 72.0% and 48.9%, respectively, with the corresponding sequences of Corymbium villosum cheravirus (CvCV). Phylogenetic analysis revealed that GCheV clustered with CvCV in the genus <i>Cheravirus</i>. These results suggest that GCheV is a new member of that genus. To the best of our knowledge, this is the first report of a virus belonging to the genus <i>Cheravirus</i> in grapevines.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of a novel negative-sense RNA virus infecting Fusarium commune, a fungus causing tobacco root rot 一种侵染烟草根腐病镰刀菌的新型负义RNA病毒的鉴定与鉴定。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-21 DOI: 10.1007/s00705-025-06341-2

Huagang Xiao, Yonghui Peng, Kai Teng, Xiangping Zhou, Yansong Xiao, Zhenyu Deng, Qi Huang, Can Wang, Lu Zheng, Jiatao Xie, Tianbo Liu

{"title":"Identification and characterization of a novel negative-sense RNA virus infecting Fusarium commune, a fungus causing tobacco root rot","authors":"Huagang Xiao, Yonghui Peng, Kai Teng, Xiangping Zhou, Yansong Xiao, Zhenyu Deng, Qi Huang, Can Wang, Lu Zheng, Jiatao Xie, Tianbo Liu","doi":"10.1007/s00705-025-06341-2","DOIUrl":"10.1007/s00705-025-06341-2","url":null,"abstract":"<div>\u0000 \u0000 <p>The fungal genus <i>Fusarium</i> includes several pathogenic species of significant agricultural importance for tobacco cultivation. In this study, we characterized a novel mycovirus, designated \"Fusarium commune mononegavirus 1\" (FcMV1), isolated from <i>Fusarium commune</i> strain JF-46. The bipartite genome of FcMV1 comprises two negative-sense RNA segments: RNA1 (6,443 nt) and RNA2 (6,301 nt). RNA1 contains a single open reading frame (ORF1, position nt 102–6098) encoding a 224.5-kDa polyprotein with two conserved functional domains: an RNA-dependent RNA polymerase (RdRp) and a mononegavirus mRNA-capping region V. RNA2 has seven putative ORFs (ORF2-ORF8), with ORF6 predicted to encode a nucleocapsid protein, while the remaining ORFs encode hypothetical proteins of unknown function. A multiple sequence alignment revealed 53.5% identity between the FcMV1 polyprotein and its counterpart in Trichoderma harzianum mononegavirus 2, with the nucleocapsid protein showing 33.4% sequence identity. A phylogenetic tree based on RdRp sequences, constructed using the maximum-likelihood method, positioned FcMV1 within a clade with members of the genus <i>Plasmopamonavirus</i> of the family <i>Mymonaviridae</i>. This is the first report of a negative-sense RNA mycovirus infecting <i>F. commune</i>, expanding our understanding of mycovirus diversity in phytopathogenic fungi.</p>\u0000 </div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The full‑length genome sequence of a novel polymycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea 一种感染植物病原真菌马铃薯球孢菌的新型多分枝病毒的全基因组序列。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-17 DOI: 10.1007/s00705-025-06330-5

Shunpei Xie, Yanfen Wang, Fengyue Gong, Haiyan Wu, Haiqiang Li, Qinzhou Ma, Yashuang Guo, Yuehua Geng, Bingshan Liu, Meng Zhang

{"title":"The full‑length genome sequence of a novel polymycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea","authors":"Shunpei Xie, Yanfen Wang, Fengyue Gong, Haiyan Wu, Haiqiang Li, Qinzhou Ma, Yashuang Guo, Yuehua Geng, Bingshan Liu, Meng Zhang","doi":"10.1007/s00705-025-06330-5","DOIUrl":"10.1007/s00705-025-06330-5","url":null,"abstract":"<div><p>Here, a novel dsRNA virus belonging to the family <i>Polymycoviridae</i> was identified in the phytopathogenic fungal strain <i>B. dothidea</i> ZM200473 and tentatively named “Botryosphaeria dothidea polymycovirus 2” (BdPmV2). The genome of BdPmV2 consists of five genomic dsRNA segments, ranging in size from 1224 to 2407 bp, each containing an open reading frame (ORF). ORF1 encodes a conserved RNA-dependent RNA polymerase (RdRP) consisting of 763 amino acids (aa) with a molecular mass (<i>Mr</i>) of 84.03 kDa, ORF3 encodes a putative methyltransferase (Met) consisting of 627 amino acids (aa) with an <i>Mr</i> of 68.41 kDa, ORF4 encodes a P-A-S-rich protein that serves as a coat protein (CP) consisting of 261 amino acids (aa) with an <i>Mr</i> of 27.60 kDa. ORF2 and ORF5 encode putative proteins with unknown functions, consisting of 697 amino acids (aa) with an <i>Mr</i> of 76.49 kDa and 323 amino acids (aa) with an <i>Mr</i> of 33.92 kDa, respectively. BLASTp analysis revealed that the RdRP protein of BdPmV2 had the highest similarity (56.52% identity) to that of a virus previously identified as \"Aspergillus fumigatus polymycovirus 1\". Phylogenetic analysis based on RdRP aa sequences indicated that BdPmV2 is a new member of the family <i>Polymycoviridae</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplex quantitative PCR assay for porcine circoviruses 2 and 3 and its application for measuring infection rates at different stages of pig production 猪圆环病毒2型和3型多重定量PCR检测及其在猪生产不同阶段感染率测定中的应用

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-16 DOI: 10.1007/s00705-025-06351-0

Zhen Li, Shuaiyin Guan, Jiaying Zhao, Yuhuan Chen, Yang Han, Ang Tian, Saisai Zhou, Huanchun Chen, Yunfeng Song

{"title":"Multiplex quantitative PCR assay for porcine circoviruses 2 and 3 and its application for measuring infection rates at different stages of pig production","authors":"Zhen Li, Shuaiyin Guan, Jiaying Zhao, Yuhuan Chen, Yang Han, Ang Tian, Saisai Zhou, Huanchun Chen, Yunfeng Song","doi":"10.1007/s00705-025-06351-0","DOIUrl":"10.1007/s00705-025-06351-0","url":null,"abstract":"<div><p>Porcine circovirus 2 (PCV2) and porcine circovirus 3 (PCV3) are considered a threat to the pig industry due to their association with growth retardation and reproductive disorders. In this study, we developed a multiplex real-time PCR (qPCR) assay for simultaneous detection of PCV2 and PCV3 and used it to investigate the epidemiology of PCV2 and PCV3 at different stages of pig production. The sensitivity of the multiplex qPCR was 4.32 × 10<sup>1</sup>copies/µL for PCV2 and 1.01 × 10<sup>1</sup>copies/µL for PCV3, and the coefficient of variation was less than 1%. The correlation coefficients (R<sup>2</sup>) of the standard curves were all greater than 0.990. Out of 1241 samples tested, nine (0.73%) were positive for PCV2, 209 (16.84%) for PCV3, and three (0.24%) for both. PCV2 was detected in saliva from asymptomatic gilts on one farm, and PCV3 was detected in all sample types except semen at all production stages on all of the farms where samples were collected. The main sample types that tested positive were saliva (23.19%, 77/322), blood (17.80%, 102/573), saliva/blood mixture (18.75%, 9/48), and pigpen swabs (32.14%, 9/28). Viral loads ranged from 10<sup>3.9</sup> to 10<sup>8.2</sup> copies/mL. Gilts (37.85%, 81/214) and grow-finish pigs (42.25%, 30/71) were the main asymptomatic PCV3 carriers. Piglets (16.67%, 3/18) and nursery pigs (22.73%, 5/22) were the main symptomatic PCV3 carriers. The assay described here is a practical and sensitive diagnostic technique for identification and monitoring of PCV2 and PCV3 infections and can provide new information on the epidemiology of PCV2 and PCV3 that can be applied for developing effective prevention and control strategies.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological characteristics of human parainfluenza virus infections and phylogenetic analysis of human parainfluenza virus type 3 isolated from children with respiratory tract infections from 2020 to 2022 in Zhejiang, China 2020 - 2022年浙江省人副流感病毒感染流行病学特征及3型人副流感病毒分离株系统发育分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-13 DOI: 10.1007/s00705-025-06343-0

Yun Lu, Jili Ni, Shuangshuang Huang, Yajun Guo, Yingying Chen, Shufa Zheng, Yingshuo Wang, Wei Li

{"title":"Epidemiological characteristics of human parainfluenza virus infections and phylogenetic analysis of human parainfluenza virus type 3 isolated from children with respiratory tract infections from 2020 to 2022 in Zhejiang, China","authors":"Yun Lu, Jili Ni, Shuangshuang Huang, Yajun Guo, Yingying Chen, Shufa Zheng, Yingshuo Wang, Wei Li","doi":"10.1007/s00705-025-06343-0","DOIUrl":"10.1007/s00705-025-06343-0","url":null,"abstract":"<div><p>Human parainfluenza viruses (HPIVs) are a major cause of pediatric respiratory hospitalizations worldwide. HPIV-3 is one of the most common subtypes of HPIV. In this study, we retrospectively analyzed the respiratory pathogen detection results for 23,775 children with ARIs who were treated in Zhejiang Province from January 2020 to December 2022, 610 HPIVs-positive specimens were selected for parainfluenza virus typing, and 35 HPIV-3-positive samples were selected for HN gene sequencing and analysis. In total, 1383 of the specimens were positive for HPIV, resulting in a detection rate of 5.82%. During this period, the detection rate was the highest in 2020, at 7.77%. The detection rate in autumn was 9.17%, surpassing those in other seasons. Among the different age groups, schoolchildren had the lowest detection rate of 1.75%. HPIV-3 was the predominant subtype, accounting for 76% of HPIV-positive cases. The nucleotide and amino acid sequence identity of the 35 HPIV-3 HN genes that were examined ranged from 97.0–100.0% and 98.6–100.0%, respectively. Phylogenetic analysis revealed that 27 isolates belonged to the C3a subtype and that eight belonged to the C3b subtype. A total of 171 nucleotide variations and 30 amino acid variations were identified among the 35 isolates. These findings confirm that HPIV, especially HPIV-3, was a significant pathogen in acute respiratory infection among pediatric patients in Zhejiang from 2020 to 2022. The observation of high sequence similarity and relatively stable genetic evolution of HPIV-3 in this region provides valuable insights into the molecular and epidemiological characteristics of this virus.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0