Archives of Virology最新文献_第6页

Molecular characterization of a novel victorivirus isolated from Stagonosporopsis citrulli 瓜穗孢子虫分离的一种新型维多利亚病毒的分子特征

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-12-12 DOI: 10.1007/s00705-024-06185-2

Xiaofang Ma, Xing Zhang, Lifeng Zhai, Yingchun Jiang, Peter Moffett, Yu Zhang, Fang Song, Xin Song, Zhijing Wang, Ligang He, Shengmei Ji, Liming Wu

引用次数: 0

Direct TaqMan assay for the detection and genotyping of bovine viral diarrhea virus types 1 and 2 直接TaqMan法检测牛病毒性腹泻病毒1型和2型并进行基因分型

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-12-12 DOI: 10.1007/s00705-024-06207-z

Shakir Ullah, Kosuke Notsu, Akatsuki Saito, Tamaki Okabayashi, Hirohisa Mekata, Norikazu Isoda, Satoshi Sekiguchi

{"title":"Direct TaqMan assay for the detection and genotyping of bovine viral diarrhea virus types 1 and 2","authors":"Shakir Ullah, Kosuke Notsu, Akatsuki Saito, Tamaki Okabayashi, Hirohisa Mekata, Norikazu Isoda, Satoshi Sekiguchi","doi":"10.1007/s00705-024-06207-z","DOIUrl":"10.1007/s00705-024-06207-z","url":null,"abstract":"<div><p>Bovine viral diarrhea (BVD), caused by bovine viral diarrhea virus (BVDV), has a significant economic impact on affected farms worldwide. For effective disease control, it is crucial to select an appropriate vaccine based on the specific genotype of BVDV. Therefore, developing a rapid and reliable assay to detect and genotype BVDV is imperative for controlling the spread of disease. In this study, we developed a TaqMan assay to detect and genotype BVDV types 1 and 2 directly in bovine serum without extraction of RNA. The direct BVDV TaqMan assay effectively detected both BVDV1 and BVDV2 with confirmed specificity and showed no cross-reactivity with any of the other viruses tested, including bovine respiratory syncytial virus, bovine coronavirus, Akabane virus, bovine herpesvirus 1, bovine parainfluenza virus 3, bovine immunodeficiency virus, and bovine leukemia virus. The assay could detect the virus in serum samples with a titer as low as 10<sup>2</sup> TCID<sub>50</sub>/mL in two out of three trials for BVDV1 and all three trials for BVDV2, indicating that its sensitivity is equivalent to that of virus isolation. Our findings represent a significant advancement in BVDV detection and typing directly from bovine serum.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-024-06207-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of the genetic diversity of citrus viroid VII using amplicon sequencing 柑橘类病毒VII遗传多样性的扩增子测序分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-12-12 DOI: 10.1007/s00705-024-06191-4

Grant A. Chambers, Andrew D.W. Geering, Daniel R. Bogema, Paul Holford, Georgios Vidalakis, Nerida J. Donovan

{"title":"Characterisation of the genetic diversity of citrus viroid VII using amplicon sequencing","authors":"Grant A. Chambers, Andrew D.W. Geering, Daniel R. Bogema, Paul Holford, Georgios Vidalakis, Nerida J. Donovan","doi":"10.1007/s00705-024-06191-4","DOIUrl":"10.1007/s00705-024-06191-4","url":null,"abstract":"<div><p>Viroids occur in plants as swarms of sequence variants clustered around a dominant variant, leading to adoption of the term ‘quasispecies’ to describe the viroid population in an individual host. The composition of the quasispecies can potentially change according to the age of the infection, the position of the leaf or branch in the canopy, and the host species. The primary aim of this study was to investigate the quasispecies concept for citrus viroid VII (CVd-VII), a recently discovered member of the family <i>Pospiviroidae</i>. Three experiments were conducted to determine factors affecting viroid variability (i) within different tissues of a lemon plant, (ii) among different plants of the same species (citron), and (iii) among different species and hybrids of citrus. Using two primer sets to produce amplicons for high-throughput sequencing, viroid population profiles were generated for each sample. The number of variants that were identified with both primer sets ranged from 2 to 13 per sample, and each sample comprised 1 to 4 major (> 10% sample) variants. The composition of variants differed in samples from different plants and among tissue types of a single plant. Single-nucleotide polymorphisms (SNPs), mostly in the form of substitutions, were the primary source of variation; in this study, SNPs were observed in approximately 10% of the viroid genome. The results of the three experiments indicate that CVd-VII follows the quasispecies model as reported for other viroids and that variability occurs in viroid populations in different tissue types and host species.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-024-06191-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome sequence of a novel bipartite begomovirus infecting butterfly pea (Clitoria ternatea L.) in India 一种感染印度蝴蝶豌豆（Clitoria ternatea L.）的新型双部begomavirus的全基因组序列。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-12-11 DOI: 10.1007/s00705-024-06203-3

Mohammad Akram, Deepender Kumar, Naimuddin Kamaal

{"title":"Complete genome sequence of a novel bipartite begomovirus infecting butterfly pea (Clitoria ternatea L.) in India","authors":"Mohammad Akram, Deepender Kumar, Naimuddin Kamaal","doi":"10.1007/s00705-024-06203-3","DOIUrl":"10.1007/s00705-024-06203-3","url":null,"abstract":"<div><p>In India, plants from the non-cultivated, horticultural, and agricultural categories are commonly infected with various begomoviruses, most of which produce yellow mosaic, bright yellow mosaic, or curling symptoms on leaves. In this study, the complete genome of a new bipartite begomovirus causing yellow mosaic disease (YMD) in butterfly pea (<i>Clitoria ternatea</i> L.) was characterized using rolling-circle amplification followed by restriction digestion, cloning, and sequencing to obtain the full-length DNA-A (2727 nt) and DNA-B (2648 nt) sequences. The DNA-A and DNA-B components have a genome organization that is typical of the Old World bipartite begomoviruses, and the common regions (95 nt) of DNA-A and DNA-B share 91% nucleotide sequence identity, which is well above the threshold (>85%) for them to be considered cognate components. Both DNA molecules contain a begomovirus nonanucleotide motif, conserved iterons, and a putative stem-loop structure. The DNA-A of this virus was found to be most similar to that of Cajanus scarabaeoides yellow mosaic virus (CsYMV), with 82.61% nucleotide sequence identity, which is far below the species demarcation threshold (< 91%) for begomoviruses, whereas the DNA-B sequence showed the most similarity (77.16% identity) to that of Rhynchosia yellow mosaic virus (RhYMV). In phylogenetic analysis, both DNA-A and DNA-B were formed a clade with the corresponding genome segments of other YMD-causing begomoviruses. Based on these characteristics, this virus, for which the name “butterfly pea yellow mosaic virus” (BpYMV) is proposed, should be considered a member of a new species in the genus <i>Begomovirus</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resolving viral structural complexity by super-resolution microscopy 用超分辨率显微镜分析病毒结构的复杂性

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-12-09 DOI: 10.1007/s00705-024-06192-3

Kevin Olaya-Bravo, Daniel Martínez-Flores, Aaron Pavel Rodríguez-Hernández, Ileana Tobías-Juárez, Jorge A. Castro-Rodríguez, Alicia Sampieri, Luis Vaca

引用次数: 0

An epizootic of highly pathogenic avian influenza virus H7N3 in a Mexican ecological reserve 墨西哥某生态保护区发生H7N3型高致病性禽流感

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-12-09 DOI: 10.1007/s00705-024-06200-6

Roberto Navarro-López, Héctor Enrique Valdez-Gómez, Mitzunari Zalapa-Hernández, Mario Solís-Hernández, Miguel Ángel Márquez-Ruiz, Abel Rosas-Tellez, Carlos Guichard-Romero, Gerardo Cartas-Heredia, Romeo Morales-Espinoza, Claudio L. Afonso, Ninnet Gómez-Romero

引用次数: 0

Molecular and ultrastructural characteristics of virulent and attenuated vaccine strains of goose parvovirus LIV-22 鹅细小病毒LIV-22强毒株和弱毒株的分子和超微结构特征

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-12-08 DOI: 10.1007/s00705-024-06188-z

Ezekiel S. Oloruntimehin, Sofya I. Volodina, Ivan V. Patraman, Grigory A. Nadtochey, Alexander S. Malogolovkin

{"title":"Molecular and ultrastructural characteristics of virulent and attenuated vaccine strains of goose parvovirus LIV-22","authors":"Ezekiel S. Oloruntimehin, Sofya I. Volodina, Ivan V. Patraman, Grigory A. Nadtochey, Alexander S. Malogolovkin","doi":"10.1007/s00705-024-06188-z","DOIUrl":"10.1007/s00705-024-06188-z","url":null,"abstract":"<div><p>The disease caused by goose parvovirus (GPV) affects young goslings and ducks and leads to substantial losses for farmers due to high mortality rates, reaching 70-100% in naive flocks. Here, we present the results of a study focusing on the historical virulent GPV LIV-22 strain, which was isolated in the USSR in 1972. An attenuated GPV LIV-22 vaccine strain that was generated by continuous passaging in goose embryonic fibroblasts was also studied. Phylogenetic analysis placed both GPV LIV-22 strains in the classical GPV group, close to the vaccine and low-pathogenic strains. However, several individual changes in the GPV LIV-22 VP1 gene highlight the uniqueness of the evolution and adaptation mechanism of GPV LIV-22 strains. Transmission electron microscopy (TEM) revealed severe ultrastructural changes in goose hepatocytes and enterocytes as early as 24-48 h postinfection, confirming abrupt GPV pathogenesis. This description of some of the essential characteristics of the GPV LIV-22 virulent and vaccine strain will be useful for studying GPV evolution and molecular pathogenesis.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142789216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterisation of novel reassortants of the G57 genotype of low-pathogenic avian influenza H9N2 virus isolated from poultry farms in Malaysia 从马来西亚家禽养殖场分离的低致病性禽流感H9N2病毒G57基因型新重组物的分子特征

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-12-07 DOI: 10.1007/s00705-024-06159-4

Erandi Gunasekara, M. Hair-Bejo, I. Aini, A. R. Omar

{"title":"Molecular characterisation of novel reassortants of the G57 genotype of low-pathogenic avian influenza H9N2 virus isolated from poultry farms in Malaysia","authors":"Erandi Gunasekara, M. Hair-Bejo, I. Aini, A. R. Omar","doi":"10.1007/s00705-024-06159-4","DOIUrl":"10.1007/s00705-024-06159-4","url":null,"abstract":"<div><p>In late 2017, Malaysia reported repeated outbreaks of low-pathogenic avian influenza virus (LPAI) H9N2 infections in commercial poultry flocks. Two H9N2 viruses, A/chicken/Malaysia/Negeri Sembilan/UPM994/2018 and A/chicken/Malaysia/Johore/UPM2033/2019, which were isolated from breeder and layer flocks in Peninsular Malaysia, were characterised in this study. Phylogenetic analysis revealed that both viruses were multiple-genotype reassortant strains with genes originating from Y280-like (HA gene), F/98-like (NS, NP and PA), G1-like (M and PB2), and Korean-like (PB1) lineages, indicating that they belong to a novel genotype that is divergent from the G57 lineage of Chinese origin. Both isolates were predicted to have a dibasic cleavage site (333-PSRSSRGLF-341) in the HA gene cleavage locations. Thus, the novel Malaysian H9N2 strain is a Y280-like virus resembling H9N2 isolates from Indonesia, Taiwan, Japan, and Cambodia. This virus is of the G57 lineage but has a novel genotype of the PB1 gene originating from a Korean-lineage H9N2 virus, which has not been detected before in the region.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142789368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome sequence of Valeriana jatamansi cryptic virus 1: a novel member of the genus Alphapartitivirus infecting Valeriana jatamansi Jones 中华绒球孢子虫隐病毒1的全基因组序列：感染中华绒球孢子虫的一种新成员

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-12-05 DOI: 10.1007/s00705-024-06194-1

Chaorong Yang, Bifan Chu, Rex Frimpong Anane, Jianqin He, Dexi Wu, Yonghong Yang, Xuehua Li, Zhongan Liu, Jianhua Wang, Jinyan Shang, Mingfu Zhao

{"title":"Complete genome sequence of Valeriana jatamansi cryptic virus 1: a novel member of the genus Alphapartitivirus infecting Valeriana jatamansi Jones","authors":"Chaorong Yang, Bifan Chu, Rex Frimpong Anane, Jianqin He, Dexi Wu, Yonghong Yang, Xuehua Li, Zhongan Liu, Jianhua Wang, Jinyan Shang, Mingfu Zhao","doi":"10.1007/s00705-024-06194-1","DOIUrl":"10.1007/s00705-024-06194-1","url":null,"abstract":"<div><p>A new double-stranded RNA (dsRNA) virus, tentatively named \"Valeriana jatamansi cryptic virus 1\" (VJCV1, GenBank accession nos. PP482519 and PP482520), was isolated from diseased <i>Valeriana jatamansi</i> Jones plants exhibiting vein-banding in Yunnan. Its complete genome sequence was determined using metatranscriptomic and Sanger sequencing. The genome of VJCV1 consists of two dsRNA of different size, namely dsRNA1 (2,026 bp) and dsRNA2 (1,754 bp), which are predicted to encode an RNA-dependent RNA polymerase (RdRp, 616 aa) with molecular weight of 72.6 kDa and coat protein (CP, 491 aa) with molecular weight of 55.8 kDa, respectively. The non-coding region of dsRNA in VJCV1 is predicted to have a stem-loop structure and a poly(A) tail that are unique to the members of the genus <i>Alphapartitivirus</i>. Multiple sequence alignments showed that the RdRp and CP of VJCV1 shared the highest amino acid sequence identity (86.2% and 56.1%, respectively) with red clover cryptic virus 1 (RCCV1). These values are below the threshold for creating new species within the genus <i>Alphapartitivirus</i>. Phylogenetic analysis based on RdRp and CP sequences showed that VJCV1 clustered independently from members of the genus <i>Alphapartitivirus</i>, with RCCV1 being the closest relative. It is therefore suggested that VJCV1 should be considered a member of a new species of the genus <i>Alphapartitivirus</i> in the family <i>Partitiviridae</i>. This is the first report of a member of the genus <i>Alphapartitivirus</i> infecting a plant of the genus <i>Valeriana</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the interaction of influenza a virus nucleoprotein with host cell nucleolin 甲型流感病毒核蛋白与宿主细胞核蛋白相互作用的分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-12-05 DOI: 10.1007/s00705-024-06189-y

Shruti Mishra, Achyut Pandey, Jyoti Verma, Maitreyi S. Rajala

引用次数: 0