Archives of Virology最新文献

{"title":"Complete Genome Sequence of Atractylodes virus A, a Novel Carlavirus Infecting Atractylodes lancea","authors":"Tingting Li,&nbsp;Hailing Zuo,&nbsp;Yuyu Liu,&nbsp;Yuezhen Liu,&nbsp;Lei Chen,&nbsp;Meng Wang,&nbsp;Xiao Huang,&nbsp;Juan Deng,&nbsp;Kun Yu","doi":"10.1007/s00705-026-06713-2","DOIUrl":"10.1007/s00705-026-06713-2","url":null,"abstract":"<div><p><i>Atractylodes lancea</i> is an important medicinal crop, but viral diseases have become increasingly severe in recent years. Viruses in the genus <i>Carlavirus</i> are common plant pathogens that are primarily transmitted by aphids and can cause stunted growth and reduced yields in their host plants. In this study, a novel carlavirus, tentatively named Atractylodes virus A (AVA), was identified from <i>A. lancea</i> plants exhibiting virus-like symptoms. The full-length genome of AVA is 8,817 nt in length and contains six open reading frames, displaying the typical genomic organization of the genus <i>Carlavirus</i>. The replicase and CP exhibit 53.43% and 56.72% amino acid identity, respectively, with those of previously characterized carlaviruses, indicating that AVA represents a novel species in the genus <i>Carlavirus</i>. This study expands our understanding of viral diversity in <i>A. lancea</i> and provides a scientific foundation for the prevention and control of viral diseases as well as for resistance breeding.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148722977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first complete genome sequence of iris yellow spot virus from South America and comparative analysis with isolates of different geographic origin","authors":"Rosana Blawid,&nbsp;Edivânio Rodrigues de Araújo,&nbsp;Leandro Delalibera Geremias,&nbsp;Dennis Knierim,&nbsp;Priscila Duarte Ramirez,&nbsp;Paolo Margaria","doi":"10.1007/s00705-026-06718-x","DOIUrl":"10.1007/s00705-026-06718-x","url":null,"abstract":"<div><p>We report the first complete nucleotide sequence of an isolate of iris yellow spot virus (IYSV; <i>Orthotospovirus iridimaculaflavi</i>) from Brazil. Isolate Br-25 was collected in 2025 in Imbuia, Santa Catarina, from symptomatic onion scapes. The genome of IYSV-Br-25 consisted of L, M and S genomic RNAs of 8879, 4866 and 3223 nt, respectively. Comparison of the L segment nucleotide (nt) sequence showed identities ~96% to most of the available L segment sequences from IYSV isolates of different geographic origin, with the exception of isolate DSMZ PV-0528, which showed 89.0% nt identity. The M and S segment nt sequences showed highest identity to an isolate recently reported from sugarbeet in Idaho, USA. <i>In silico</i>-generated <i>Hinf</i>1 digestion profile assigned the isolate to the previously defined IYSV_BR genotype. This work provides valuable novel information on the genetic diversity of IYSV.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-026-06718-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148705183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and classification of a novel bacteriophage, vB_RsoP_HXg1W, infecting Ralstonia pseudosolanacearum: a new member of the genus Serkorvirus in the family Autotranscriptaviridae","authors":"XiaoLi Yu,&nbsp;Zheng Fang,&nbsp;Qingshan Wu,&nbsp;Rui Wu,&nbsp;Chuangen Lin,&nbsp;Lan Xiang,&nbsp;Qiuping Liu,&nbsp;Leitao Tan,&nbsp;Qingbei Weng","doi":"10.1007/s00705-026-06711-4","DOIUrl":"10.1007/s00705-026-06711-4","url":null,"abstract":"<div><p>Bacterial wilt caused by <i>Ralstonia solanacearum</i> species complex (RSSC) is a significant threat to agriculture due to the pathogen’s broad host range and persistence in soil. Bacteriophages (phages) are being explored as biocontrol agents, and such strategies are gaining interest. In this study, we isolated and characterized vB_RsoP_HXg1W, a novel phage propagated on <i>R. pseudosolanacearum</i> GMI1000. The phage has a linear double-stranded DNA genome of 40,184 bp with a GC content of 62.3%, and displays an icosahedral head with a short tail. A total of 51 open reading frames (ORFs) were identified, and no tRNA genes were detected. Comparative genomic and phylogenetic analyses revealed that vB_RsoP_HXg1W is closely related to <i>Ralstonia</i> phage p2137, p2106, and RpY2, and clusters within the genus <i>Serkorvirus</i> in the family <i>Autotranscriptaviridae</i>. VIRIDIC analysis revealed a maximum intergenomic similarity of 84.7% to the closest included relative, supporting vB_RsoP_HXg1W as a putative novel species-level member of <i>Serkorvirus</i>. These findings contribute to the understanding of RSSC-infecting phages and provide a foundation for further exploration of phage evolution, host range, and biocontrol relevant traits.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148705261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Emergence of norovirus GII.7 and predominance of recombinant strains in pediatric acute gastroenteritis in Japan, 2021–2024","authors":"Setsuko Imakado,&nbsp;Ngan Thi Kim Pham,&nbsp;Pattara Khamrin,&nbsp;Yuko Shimizu-Onda,&nbsp;Sheikh Ariful Hoque,&nbsp;Quang Duy Trinh,&nbsp;Mudassar Hussain,&nbsp;Arpaporn Yodmeeklin,&nbsp;Kattareeya Kumthip,&nbsp;Shuichi Nishimura,&nbsp;Masaaki Kobayashi,&nbsp;Kumiko Sugita,&nbsp;Shoko Okitsu,&nbsp;Niwat Maneekarn,&nbsp;Satoshi Hayakawa,&nbsp;Shihoko Komine-Aizawa,&nbsp;Kazuaki Yoshimune,&nbsp;Hiroshi Ushijima","doi":"10.1007/s00705-026-06688-0","DOIUrl":"10.1007/s00705-026-06688-0","url":null,"abstract":"","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148695598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of feline kobuvirus in filtered fecal inocula and molecular epidemiological characterization of feline kobuvirus in Japan","authors":"Tomomi Takano,&nbsp;Yuki Tanimoto,&nbsp;Hajime Kusuhara,&nbsp;Tomoyoshi Doki","doi":"10.1007/s00705-026-06708-z","DOIUrl":"10.1007/s00705-026-06708-z","url":null,"abstract":"<div><p>Feline kobuvirus (FKoV) is an enteric virus detected in cats in several countries, but its prevalence and genomic characteristics in Japan remain unclear. Here, we report the detection of FKoV in specific-pathogen-free (SPF) cats inoculated with filtered fecal material that had tested negative for FKoV by an initial RT-PCR assay. The inoculum was derived from a shelter-housed cat positive for a feline norovirus (FNoV) strain (KU22; GVI.1; GenBank accession no. LC935948), which shared 99% VP1 nucleotide identity with the Japanese strain M49-1. FKoV RNA was undetectable in all four inoculated cats at day 0 using a degenerate universal kobuvirus primer set, but was detected in all four cats at days 5, 20, and 30 post inoculation. FKoV RNA in the inoculum was negative by the initial RT-PCR assay but positive by the degenerate universal kobuvirus primer set, indicating that FKoV was present in the inoculum but was missed by the initial assay; FKoV RNA was subsequently detected in the inoculated cats, consistent with inoculum-associated introduction of FKoV. Near-complete genome sequencing confirmed that the Japanese strain belonged to the feline kobuvirus lineage, representing the first near-complete FKoV genome sequence from Japan. In a field survey of 83 domestic cats, FKoV RNA was detected in 10 samples (12.0%), with higher prevalence in cats with gastroenteritis than in apparently healthy cats (40.0% vs. 8.2%; Bonferroni-adjusted <i>p</i> = 0.049), and exclusively in cats younger than 1 year. These findings provide genomic and epidemiological data on FKoV in Japan and highlight the need for broad-spectrum screening of biological inocula used in experimental infection studies.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148683209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visual typing detection of bovine leukemia virus with a lateral flow immunoassay based on latex microspheres","authors":"Jiashu Hu,&nbsp;Haiyan He,&nbsp;Kun Chen,&nbsp;Zhijun Li,&nbsp;Aoxue Wang,&nbsp;Lulu Zhang,&nbsp;Fanhua Wei","doi":"10.1007/s00705-026-06710-5","DOIUrl":"10.1007/s00705-026-06710-5","url":null,"abstract":"<div><p>Bovine Leukemia Virus (BLV), a member of the genus <i>Deltaretrovirus</i>, cause chronic tumor-related diseases in bovine and posing a significant threat to bovine industry. Currently, the detection of BLV mainly relies on agar gel immunodiffusion (AGID) and enzyme-linked immunosorbent assay (ELISA). However, these methods are limited by their complex procedures, long processing times, and high costs, making them unsuitable for rapid onsite diagnosis. In this study, a lateral flow immunoassay (LFIA) was established based on the BLV gp51 protein employing latex microsphere (LM) as signal labels was developed for the rapid and sensitive detection of bovine derived BLV. This method can detect the positive standard serum at a dilution of 1:256. A parallel analysis in 138 bovine serum samples were confirmed by LFIA and commercial ELISA kits, showed strong correlation with reference methods and no cross-reactivity with other bovine pathogens. The LFIA exhibited high relative sensitivity (87.09%) and specificity (99.06%), and concordance rate of the LFIA and ELISA was 96.37% (kappa = 0.89), indicating the practical reliability of the LFIA. The LFIA remained consistent accuracy and functionality at least 90-day at 4 ℃ or 70-day at 37 ℃. Our proposed approach provided an efficient solution for rapid on-site and visual screening in resource limited environment Therefore, the LFIA has a great potential for BL diagnosis and prevention.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148683226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of endogenous pararetroviruses in yam (Dioscorea spp.) genomes revealed four pararetrovirus groups, including a dioscovirus-like lineage: implications for diagnostics and yam germplasm exchange","authors":"B. O. Osundahunsi,&nbsp;L. Moshood,&nbsp;O. A. Kolade,&nbsp;B. O. Odu,&nbsp;P. Lava Kumar","doi":"10.1007/s00705-026-06703-4","DOIUrl":"10.1007/s00705-026-06703-4","url":null,"abstract":"<div><p>Yams (<i>Dioscorea</i> spp.) are an important vegetatively propagated food security crop grown for their starchy tubers. Yams are susceptible to several viruses, and their genomes harbor a diverse array of endogenous pararetroviral sequences (EPRVs), which complicate diagnostics and germplasm exchange because of their similarity to episomal viruses. To better characterize EPRV diversity, we analyzed 86 publicly available whole-genome sequences from five <i>Dioscorea</i> species (<i>D. rotundata</i>,<i> D. alata</i>,<i> D. praehensilis</i>,<i> D. abyssinica</i>, and <i>D. dumetorum</i>). Assembled genomes were screened for endogenous pararetrovirus sequences (EPRVs) using the CAULIFINDER pipeline, targeting conserved RT/RNase H domains of the <i>Caulimoviridae</i> family. Our analyses revealed four major EPRV groups in <i>D. rotundata</i>: Yendovirus, Badnavirus, Yam Endovirus 1, and a Dioscovirus-like lineage. While most insertions were fragmented, we found full-length putative viral genomes corresponding to the Yam Badnavirus and Dioscovirus-like clades. These findings expand knowledge of yam EPRV diversity, contribute to the development of improved diagnostic tools to differentiate endogenous and episomal forms, and promote a science-based, risk-proportionate approach to yam phytosanitation that facilitates global germplasm exchange while maintaining biosecurity.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-026-06703-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148683180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiviral efficacy of Trappin-2/Elafin against Zika virus in the human keratinocyte HaCaT cell line","authors":"Víctor Javier Cruz-Holguín,&nbsp;José Manuel Ulloa-Aguilar,&nbsp;Rocío Yazmin Benítez-Zeferino,&nbsp;Luis Herrera- Moro Hiutron,&nbsp;Addy Cecilia Helguera-Repetto,&nbsp;Luis Adrián de Jesús-González,&nbsp;Guadalupe León-Reyes,&nbsp;Julio Garcia-Cordero,&nbsp;Sergio Aguilar-Ruiz,&nbsp;Nicolas Villegas-Sepulveda,&nbsp;Leticia Cedillo-Barrón,&nbsp;Moisés León-Juárez","doi":"10.1007/s00705-026-06706-1","DOIUrl":"10.1007/s00705-026-06706-1","url":null,"abstract":"<div><p>Zika virus (ZIKV) is primarily transmitted through mosquito bites, and the skin acts as the initial site of viral entry into the host. Consequently, resident skin cells are among the first targets of infection. The epidermis, mainly composed of keratinocytes, can mount an antiviral response against arboviruses through the production of interferons, interferon-stimulated genes, cytokines, and antimicrobial peptides (AMPs), including the Trappin-2/Elafin (Tr2/E) peptide. However, the antiviral activity of Tr2/E during ZIKV infection remains poorly understood, therefore, this study aimed to investigate the antiviral activity of Tr2/E in human keratinocytes during ZIKV infection. In this study, we evaluated the permissiveness of the human keratinocyte cell line HaCaT to infection with a Mexican isolate of ZIKV and observed that these cells support productive viral infection. We then assessed whether ZIKV infection induces endogenous expression of Tr2/E. Tr2/E transcripts were detected in infected cells and showed increased expression over time post-infection, which correlated with the presence of its corresponding protein. Furthermore, we evaluated the antiviral potential of this peptide through exogenous treatment of infected keratinocytes. A significant reduction in ZIKV infection following Tr2/E treatment was observed. Collectively, these findings provide additional insight into the involvement of AMPs in the antiviral response to ZIKV infection and highlight Tr2/E as a potential antiviral factor.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148683146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of KL29 type acinetobacter baumannii phage of obolenskvirus subfamily and tail-associated depolymerase activity","authors":"Jinpan Wei,&nbsp;Tao Yu,&nbsp;Qiang Wang,&nbsp;Can Wang,&nbsp;Xuefeng Li,&nbsp;Guoping Lu,&nbsp;Lijuan Li,&nbsp;Aihui Xu,&nbsp;Lei Zhao","doi":"10.1007/s00705-026-06664-8","DOIUrl":"10.1007/s00705-026-06664-8","url":null,"abstract":"<div><p>The emergence of multidrug-resistant <i>Acinetobacter baumannii</i> (MDR-AB) as a critical nosocomial pathogen, driven by its remarkable capacity for acquired antibiotic resistance and clonal dissemination, underscores an urgent need for novel therapeutic interventions. This study characterizes a novel depolymerase derived from bacteriophage vB_AbaM_IME284, previously isolated against Ab333. Genomic analysis revealed taxonomic classification within the <i>Caudoviricetes</i> class and <i>Obolenskvirus</i> subfamily. Notably, plaque halo formation indicated polysaccharide depolymerase activity, prompting functional investigation of ORF49-encoded protein (designated Dp49). Recombinant Dp49 exhibited both depolymerase and antibacterial activities, demonstrating efficacy in biofilm inhibition and eradication. Furthermore, synergistic bactericidal effects were observed when combining Dp49 with human serum components (6-log reduction in viable counts). These findings position Dp49 as a promising therapeutic candidate for combating biofilm-associated MDR-AB infections. As a priority pathogen listed by the WHO, A. baumannii poses formidable challenges in clinical management through its rapid acquisition of multidrug resistance mechanisms and notorious capacity for forming recalcitrant biofilms that severely compromise conventional antimicrobial interventions. Our study characterizes Dp49, a newly discovered bacteriophage-derived depolymerase exhibiting potent enzymatic activity against bacterial exopolysaccharides. This enzyme demonstrates therapeutic potential by enhancing serum-mediated bactericidal activity and anti-biofilm activity. These findings not only expand the therapeutic arsenal against pan-drug resistant Gram-negative pathogens but also provide crucial mechanistic insights into phage enzyme applications. The demonstrated biofilm-disrupting capacity of Dp49 underscores the evolving paradigm of phage-derived biologics as precision anti-infectives, representing a strategic imperative in antimicrobial research given the critical void in effective therapies for extensively drug-resistant infections.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148668267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Phylogenetic analysis, genetic diversity, and epidemiology of pigeon paramyxovirus type 1 in China","authors":"Chuqi Huang,&nbsp;Shaoyu Tu,&nbsp;Wei Sheng,&nbsp;Zhihao Wang,&nbsp;Zhipeng Lin,&nbsp;Jing Qian,&nbsp;Jiahui Zou,&nbsp;Hongbo Zhou","doi":"10.1007/s00705-026-06699-x","DOIUrl":"10.1007/s00705-026-06699-x","url":null,"abstract":"","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148628699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}