Archives of Virology最新文献_第7页

Characterization of a virulent phage, P12L (genus Drulisvirus), targeting Klebsiella pneumoniae capsule type K2 针对肺炎克雷伯菌胶囊型K2的强毒噬菌体P12L （Drulisvirus属）的鉴定

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-16 DOI: 10.1007/s00705-025-06320-7

Wendy Dayang Rollon, Tay Darren Dean, Sharifah Ishaziah Mohd Idris, Nurzafirah Mazlan, Afif Pranaya Jati, Tze Young Thung

引用次数: 0

Molecular characterization of a novel mitovirus from the edible fungus Pleurotus pulmonarius 一种新型食用菌肺侧耳有丝分裂病毒的分子特征

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-16 DOI: 10.1007/s00705-025-06310-9

Zhe Wang, Junbo Xiao, Hanzhao Liu, Ziru Zheng, Yifei Zhang, Yingying Liu, Pengfei Li

引用次数: 0

Identification of pattern recognition receptors participating in the innate immune response to Tembusu virus 参与坦布苏病毒先天免疫应答的模式识别受体的鉴定

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-15 DOI: 10.1007/s00705-025-06307-4

Jingjing Huang, Xiaoyang Hu, Duo Peng, Qiong Li, Yinuo Song, Dabing Zhang

引用次数: 0

Association of grapevine geminivirus a isolate QN with upward leaf curling, necrosis, chlorosis, and malformation symptoms in two grapevine cultivars 葡萄双叶病毒a分离QN与两个葡萄品种向上卷曲、坏死、褪绿和畸形症状的关系

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-15 DOI: 10.1007/s00705-025-06318-1

Can Liu, Jinying Wang, Shangzhen Yu, Yinshuai Xie, Hanwei Li, Chenlu Feng, Mengqi Ma, Yixuan Wang, Yuqin Cheng

{"title":"Association of grapevine geminivirus a isolate QN with upward leaf curling, necrosis, chlorosis, and malformation symptoms in two grapevine cultivars","authors":"Can Liu, Jinying Wang, Shangzhen Yu, Yinshuai Xie, Hanwei Li, Chenlu Feng, Mengqi Ma, Yixuan Wang, Yuqin Cheng","doi":"10.1007/s00705-025-06318-1","DOIUrl":"10.1007/s00705-025-06318-1","url":null,"abstract":"<div><p>Grapevine geminivirus A (GGVA) is a recently identified DNA virus that infects grapevine, but its pathogenicity is not well studied. Previously, we investigated the pathogenicity of the new GGVA isolate QN (GGVA<sup>QN</sup>) on grapevine ‘Red Globe’ plants. In this study, grapevine ‘Red Globe’ and ‘Summer Black’ plants were inoculated with GGVA<sup>QN</sup> and grown without artificial control of temperature and light, and all of them exhibited upward leaf curling. This symptom was also found in field-grown grapevine ‘Shine Muscat’ plants infected with multiple grapevine viruses, including GGVA (isolate SM) (2,905 nt, sharing 97.69% genome sequence identity with GGVA<sup>QN</sup>), and in the upper developed leaves of previously GGVA<sup>QN</sup>-inoculated ‘Red Globe’ plants one week after they were moved from artificial to natural lighting. The upward leaf curling symptom was similar to that observed previously in <i>Nicotiana benthamiana</i> plants inoculated with GGVA<sup>QN</sup>, in field-grown grapevine ‘Queen Nina’ plants, and in <i>in vitro</i>-grown grapevine ‘Marselan’ plantlets, from which GGVA<sup>QN</sup> and GGVA<sup>ML</sup> isolates with identical genome sequences were obtained. In addition to upward leaf curling, malformation, chlorosis, and necrosis symptoms were also observed on all GGVA<sup>QN</sup>-inoculated ‘Red Globe’ and ‘Summer Black’ plants. The findings presented here suggest that GGVA<sup>QN</sup> is closely associated with severe foliar symptoms in grapevine ‘Red Globe’ and ‘Summer Black’ cultivars and emphasize the need to control the virus.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological status and genome characteristics of canine parvovirus in China from 2022 to 2024 2022 - 2024年中国犬细小病毒流行病学现状及基因组特征

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-11 DOI: 10.1007/s00705-025-06301-w

Haoqi Li, Hu Zhou, Linfeng Li, Xiaohu Han, Qinghua Shang, Chengguang Zhang, Ming Zhou, Zhen F. Fu, Jianqing Zhao, Ling Zhao

{"title":"Epidemiological status and genome characteristics of canine parvovirus in China from 2022 to 2024","authors":"Haoqi Li, Hu Zhou, Linfeng Li, Xiaohu Han, Qinghua Shang, Chengguang Zhang, Ming Zhou, Zhen F. Fu, Jianqing Zhao, Ling Zhao","doi":"10.1007/s00705-025-06301-w","DOIUrl":"10.1007/s00705-025-06301-w","url":null,"abstract":"<div><p>Canine parvovirus (CPV) is a highly contagious and lethal virus that primarily affects dogs. The virulence and transmissibility of this virus have intensified over time, posing significant challenges to the prevention and control of this pathogen. In this study, to investigate the epidemiology of CPV in China, 2,654 fecal samples were collected from seven provinces, and the positive rate was found to be 50.53%. The positive rates in Liaoning, Shandong, Hubei, Hunan, Guangxi, Jiangxi, and Fujian provinces were 78.53%, 9.25%, 58.37%, 3.00%, 42.68%, 78.26%, and 41.47%, respectively. Among these samples, four genotypes were identified: CPV-2 (4.42%), CPV-2c (69.04%), new CPV-2a (23.81%), and new CPV-2b (2.7%). Notably, CPV-2c emerged as the most prevalent genotype. Amino acid sequence alignments indicated that the main substitutions in VP2 were M87L, S297A, A300G, Y324I, N375D, and N426D/E, and the main substitutions in NS1 were I60V, Y544F, E545V, and L630P. Moreover, the mutations A5G, Q370R, and N426E were observed to be nearly universal among all CPV-2c strains, and I60V, Y544F, E545V were mainly found in CPV-2c and new CPV-2b strains. Phylogenetic analysis based on VP2 sequences revealed that CPV and FPV formed two distinct clusters. Furthermore, the CPV cluster was subdivided into four groups: CPV-2, CPV-2c, new CPV-2a, and new CPV-2b. However, the sub-branch of the NS1 evolutionary tree did not correspond strictly to the CPV genotype. In summary, our findings revealed significant variation in the prevalence of CPV across different regions in China. These findings carry significant implications for our understanding of CPV transmission dynamics and will play an important role in informing vaccine development strategies. </p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenomic analysis of African swine fever virus from the Philippines 菲律宾非洲猪瘟病毒的系统基因组分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-10 DOI: 10.1007/s00705-025-06300-x

Szarina Krisha K. Ko, Rohani Cena-Navarro, Ma Jowina H. Galarion, Gabriela Ilona B. Janairo, Adrian B. Abarientos, Rachel R. Azul, Cristina F. Legaspi, Jennifer L. Maravilla, Brian E. Schwem

引用次数: 0

Trirhavirus: the first genus of tripartite viruses in the family Rhabdoviridae 三叉病毒：横纹肌病毒科三叉病毒的第一属

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-09 DOI: 10.1007/s00705-025-06321-6

Nicolas Bejerman, Humberto Debat

引用次数: 0

A novel varicosavirus associated with Orychophragmus violaceus in China 中国一种与紫斑田鼠相关的新型静脉曲张病毒

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-07 DOI: 10.1007/s00705-025-06283-9

Yuanling Chen, Qian Li, Jiaping Yu, Yali Zhou, Shifang Fei, Jianxiang Wu, Shuai Fu

{"title":"A novel varicosavirus associated with Orychophragmus violaceus in China","authors":"Yuanling Chen, Qian Li, Jiaping Yu, Yali Zhou, Shifang Fei, Jianxiang Wu, Shuai Fu","doi":"10.1007/s00705-025-06283-9","DOIUrl":"10.1007/s00705-025-06283-9","url":null,"abstract":"<div><p>Plant rhabdoviruses have garnered considerable attention due to their broad host range and significant agricultural threats. Despite their prevalence in various ecosystems, rhabdoviruses in non-crop species, which often serve as reservoirs for novel viral strains, remain understudied. Here, a novel plant rhabdovirus, Orychophragmus violaceus varicosavirus (OVVV), was identified in the ornamental plant <i>Orychophragmus violaceus</i> and characterized by high-through sequencing. OVVV has a bisegmented genome, with RNA1 consisting of 6,795 nucleotides (nt) and RNA2 consisting of 5,895 nt. The L protein encoded by RNA1 shares 36.4–67.2% amino acid sequence identity with those of known varicosaviruses. The four proteins encoded by RNA2 share 19.5–53.4% amino acid identity with the corresponding proteins of known varicosaviruses. Sequence comparisons and phylogenetic analysis indicated that OVVV is a novel RNA virus representing a new species within the genus <i>Varicosavirus</i>. This discovery not only expands the known host range of varicosaviruses but also offers new insights into the molecular evolution of this group of viruses.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143913944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of hepatitis C virus replication in HepG2 cells via modulation of the Raf-1 and interferon-alpha signaling pathways by thymoquinone 百里醌通过调节Raf-1和干扰素- α信号通路抑制丙型肝炎病毒在HepG2细胞中的复制

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-03 DOI: 10.1007/s00705-025-06294-6

Rasha Sleem, Ahmed Salah, Amal Abd Alziz, Ahmed A. Daif, Ahmed A. Abdel Megeed, Hany Khalil

{"title":"Inhibition of hepatitis C virus replication in HepG2 cells via modulation of the Raf-1 and interferon-alpha signaling pathways by thymoquinone","authors":"Rasha Sleem, Ahmed Salah, Amal Abd Alziz, Ahmed A. Daif, Ahmed A. Abdel Megeed, Hany Khalil","doi":"10.1007/s00705-025-06294-6","DOIUrl":"10.1007/s00705-025-06294-6","url":null,"abstract":"<div><p>Hepatitis C virus (HCV) infection is a significant global health concern, as both acute and chronic hepatitis caused by HCV can lead to liver cancer and long-term liver damage. Thymoquinone (TQ), the active compound found in the remarkable herb <i>Nigella sativa</i>, has various anti-inflammatory and antiproliferative effects. In this study, we investigated the effect of TQ on the interferon-alpha (IFN-α) pathway and its ability to prevent HCV replication in the HepG2 cell line. Our findings showed no significant alterations in cell viability or lactate dehydrogenase (LDH) production in TQ-treated cells, while significant alteration in both factors was detected in cells treated with Sovaldi, the most commonly used drug for treatment of HCV infection. Interestingly, the level of the HCV NS5A protein was significantly reduced in infected cells treated with either TQ or Sovaldi in a dose-dependent manner. The expression of phosphorylated Raf-1 (phospho-Raf-1) and phospho-Mek-1 in infected cells was inhibited by TQ treatment, and the potential interaction between TQ and Ref-1 was confirmed by a molecular docking simulation. Unlike autophagy-related 12 (Atg12), the expression of LC3B in infected cells was also inhibited in a dose-dependent manner by TQ treatment. Conversely, the levels of interleukin-27 (IL-27) and interferon-alpha (IFN-α) in infected cells were significantly increased in a time- and dose-dependent manner by TQ treatment. These data suggest that TQ exerts antiviral effects in HepG2 cells by disrupting HCV replication through targeting of the Raf-1 signaling pathway and promoting the overproduction of IL-27 and IFN-α in infected cells.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotypes, subtypes, and genetic variability of hepatitis B virus from blood donors in Timor-Leste 东帝汶献血者乙型肝炎病毒的基因型、亚型和遗传变异

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-01 DOI: 10.1007/s00705-025-06305-6

Hendriketa da Silva, Juniastuti, Mochamad Amin, Januari Soares, Miguel Soares, Hitler Malik, Antonio Ximenes, Maria Bela, Bernadino Fernandes

{"title":"Genotypes, subtypes, and genetic variability of hepatitis B virus from blood donors in Timor-Leste","authors":"Hendriketa da Silva,  Juniastuti, Mochamad Amin, Januari Soares, Miguel Soares, Hitler Malik, Antonio Ximenes, Maria Bela, Bernadino Fernandes","doi":"10.1007/s00705-025-06305-6","DOIUrl":"10.1007/s00705-025-06305-6","url":null,"abstract":"<div><p>Timor-Leste experiences high hepatitis B endemicity; however, information about hepatitis B virus (HBV) variants in Timor-Leste is still limited. In this study, we determined genotypes and subtypes and identified mutations in the surface (S), polymerase (P), basal core promoter (BCP), precore (PC), and core (C) genes of HBV isolates from blood donors in Timor-Leste. Sera were examined using serological tests and PCR sequencing. Out of 127 sera tested, 38 (30%) were positive for the hepatitis B S antigen (HBsAg). Thirty-eight sequences of the S and P genes, 22 sequences of the BCP and PC regions, and 23 sequences of C genes were determined and analyzed. The most common genotype/subtype was C/<i>adrq+</i>, followed by B/<i>ayw1</i>, B/<i>adw2</i>, and C/<i>adw2</i>. Several mutations in the S protein that are associated with vaccine escape were identified in samples of genotype C (I110L, S113T, T126I, T143S, Y161F) and B (K122R), some of which might have been from vaccinated individuals. None of the healthy carriers had taken anti-HBV drugs, but one was infected with a virus with a mutation in the P gene associated with anti-HBV drug resistance (Y141F). The mutations A1762T and G1764A in BCP were detected in 18.1-22.7% of the samples. In the PC region, the mutation C1858T was the most frequent, followed by G1896A and G1899A. In the C gene, 13 mutations (P5T, T67N, E77Q, P79Q/A, E83D, V91T, I97L/F, L116I, and P130I/P/T) associated with severe liver disease were identified. Viruses obtained from four healthy carriers who were later found to have died of hepatocellular carcinoma also showed those mutations. In conclusion, among blood donors in Timor-Leste, HBV genotype/subtype C/<i>adrq+</i> and several mutations in the S and C genes were prevalent. Routine implementation of a national immunization program and monitoring of disease progression in healthy carriers should be considered.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0