Archives of Virology最新文献_第7页

Characterization and genetic analysis of two sugarlandviruses: Klebsiella variicola lytic phages vB_PZK-KV7 and vB_PZK-KV23 两种糖地病毒：水痘克雷伯菌裂解噬菌体vB_PZK-KV7和vB_PZK-KV23的鉴定与遗传分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-12 DOI: 10.1007/s00705-025-06331-4

Le Zhong, Yunqian Sun, Yunhao Sun, Guohui Yu, Ping Cheng, Jinshui Zheng, Donghai Peng, Ming Sun

引用次数: 0

Construction of a shortened autographa californica multiple nucleopolyhedrovirus genome as protein expression vector 加州签名多核多角体病毒短基因组蛋白表达载体的构建。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-12 DOI: 10.1007/s00705-025-06349-8

Ziqian Cao, Xinyu Liu, Jianchao Li, Yu Zheng, Jun Yin, Hongyu Wang, Xiaoyue Zhang, Hongying Chen

{"title":"Construction of a shortened autographa californica multiple nucleopolyhedrovirus genome as protein expression vector","authors":"Ziqian Cao, Xinyu Liu, Jianchao Li, Yu Zheng, Jun Yin, Hongyu Wang, Xiaoyue Zhang, Hongying Chen","doi":"10.1007/s00705-025-06349-8","DOIUrl":"10.1007/s00705-025-06349-8","url":null,"abstract":"<div>The baculovirus expression system (BEVS) is a powerful tool for protein production in insect cells, and the most widely used virus vector in the BEVS is Autographa californica multiple nucleopolyhedrovirus (AcMNPV), whose genome contains 155 open reading frames (ORFs), nearly half of which are nonessential for viral replication in vitro. Our previous studies have shown that deletion of some large nonessential fragments from the AcMNPV genome can significantly improve the efficiency of production of foreign proteins and that the viral vector can be shortened by more than 10 kb. Using a previously described shortened vector, four additional fragments containing multiple nonessential genes (ac58-61, ac110-114, ac116-119, and ac121-124) were removed from the AcMNPV genome, and the effects on baculovirus replication and foreign protein expression were examined. The results showed that deletion of one of the nonessential fragments did not affect virus replication. For the expression of foreign proteins, removal of ac110-114 and ac116-119 was beneficial for protein production, deletion of ac121-124 apparently did not affect protein production, and deletion of ac58-61 severely impaired protein expression. Simultaneous deletion of two fragments from the AcMNPV genome significantly influenced exogenous protein expression and/or virus replication. Finally, by injection into Spodoptera frugiperda larvae, it was shown that the three shortened AcMNPVs that had maintained their infectivity and ability to express foreign proteins in cultured cells also retained their infectivity in vivo. The three shortened baculovirus expression vectors obtained in this study were 127-129 kb in length, representing a further reduction of 1.4-2.3% in genome size compared to the previously shortened vector, further optimizing it for protein production in the BEVS.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insertion of the P gene of spring viremia of carp virus into the snakehead rhabdovirus genome increases virulence 将鲤鱼病毒春季毒血症P基因插入到蛇头横纹肌病毒基因组中，毒力增强。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-12 DOI: 10.1007/s00705-025-06347-w

Mariem Bessaid, Ki Hong Kim

{"title":"Insertion of the P gene of spring viremia of carp virus into the snakehead rhabdovirus genome increases virulence","authors":"Mariem Bessaid, Ki Hong Kim","doi":"10.1007/s00705-025-06347-w","DOIUrl":"10.1007/s00705-025-06347-w","url":null,"abstract":"<div>Spring viremia of carp virus (SVCV) is highly lethal in common carp (Cyprinus carpio) and other cyprinid fish species. In this study, we evaluated the effect of the SVCV phosphoprotein (P protein) on the virulence of snakehead rhabdovirus (SHRV) by generating a recombinant SHRV expressing the SVCV P protein. The SVCV P gene was inserted between the N and P genes of the SHRV genome, resulting in the generation of rSHRV-A-Psvcv, which contains not only its own P gene but also the P gene of SVCV. In preliminary experiments, we determined the minimum titer of SHRV that was lethal in zebrafish (Danio rerio). We then conducted experiments using titers below this threshold to assess the in vivo virulence of rSHRV-A-Psvcv in zebrafish. Under these conditions, no mortality was observed in fish infected with rSHRV-A-eGFP, a modified SHRV containing the enhanced green fluorescent protein (eGFP) gene between the N and P genes, which was used as a control. However, fish infected with rSHRV-A-Psvcv exhibited mortality accompanied by hemorrhage and ophthalmia. In a minigenome assay, the SVCV P protein failed to induce reporter protein expression when co-expressed with the SHRV N and L proteins, suggesting that the SVCV P protein is unable to form a ribonucleoprotein complex with SHRV N and L proteins. Therefore, the enhanced virulence of rSHRV-A-Psvcv resulting from the addition of the SVCV P gene can probably be attributed to the intrinsic properties of the SVCV P protein itself. Epithelioma papulosum cyprini (EPC) cells infected with rSHRV-A-Psvcv exhibited significantly lower type I interferon (IFN) responses compared to those infected with rSHRV-A-eGFP, suggesting that the SVCV P protein expressed by the recombinant SHRV inhibits type I IFN responses. Thus, the immune-suppressive activity of the SVCV P protein might be one of the causes of the enhanced virulence of rSHRV-A-Psvcv.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and evaluation of a recombinant duck enteritis virus expressing the envelope protein of duck Tembusu virus in ducks 表达鸭天布苏病毒包膜蛋白的重组鸭肠炎病毒的构建与评价。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-10 DOI: 10.1007/s00705-025-06342-1

Lihua Wang, Xiaoyue Yang, Bing Zhang, Siyuan Hao, Yafen Song, Dan Li, Min Zhang, Qianyi Zhang, Yongda Zhao, Chenghuai Yang

引用次数: 0

Complete genome sequence of a novel narnavirus with inverted complementary termini from the rice blast fungus Magnaporthe oryzae isolate NJ471 稻瘟病菌Magnaporthe oryzae分离株NJ471具有反向互补末端的新型narnavvirus全基因组序列。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-08 DOI: 10.1007/s00705-025-06337-y

Cong Li, Yuxin Wu, Xinyi Li, Qingchao Deng, Jiatao Xie

引用次数: 0

Single-particle quantification of SARS-CoV-2 virus-like particles using flow virometry 用流动病毒学对SARS-CoV-2病毒样颗粒进行单颗粒定量。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-07 DOI: 10.1007/s00705-025-06339-w

Hyeop Jin, Ye Rae Cho, Yong Tae Jung

{"title":"Single-particle quantification of SARS-CoV-2 virus-like particles using flow virometry","authors":"Hyeop Jin, Ye Rae Cho, Yong Tae Jung","doi":"10.1007/s00705-025-06339-w","DOIUrl":"10.1007/s00705-025-06339-w","url":null,"abstract":"<div>SARS-CoV-2 virus-like particles (VLPs) were generated by co-transfecting HEK 293T cells with four plasmids, two for expression of the spike (S) or nucleocapsid (N) protein with a 3xHA tag at the C-terminus, one for expression of the membrane (M) protein, and one for expression of the envelope (E) protein. Co-localization of the S and N proteins within the VLPs was confirmed by Western blot analysis using anti-HA antibodies. To enable fluorescent quantification of VLPs, the C-terminus of the S protein was fused to enhanced green fluorescent protein (EGFP), and the N protein was tagged with EGFP at either the N- or C-terminus. Transient transfection of 293T cells with (S-GFP)NME, S(N-GFP)ME, or S(GFP-N)ME plasmids efficiently produced fluorescent VLPs, each demonstrating the ability to enter A549-hACE2 cells. In addition, a two-plasmid system was developed to simplify fluorescent VLP production by incorporating internal ribosomal entry site elements between N3xHA and S-GFP and between the M and E genes. Transfection of 293T cells with these plasmids produced VLPs with a fourfold higher N protein concentration (800 ng/mL) compared to the four-plasmid system (200 ng/mL), as measured by ELISA. Flow virometry analysis confirmed production of VLPs with an average diameter of 80 nm and a concentration of 1.68 × 108 particles/ml. This study demonstrates that production of fluorescent VLPs using a two-plasmid system is more efficient than the traditional four-plasmid approach. We also demonstrate that flow virometry is a robust method for single-particle analysis of fluorescent VLPs for determining their size and concentration.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of chenopodium trirhavirus 1, a novel tri-segmented virus of the family Rhabdoviridae 横纹肌病毒科一种新型三节段病毒chenopodium trirhavirus 1的鉴定。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-07 DOI: 10.1007/s00705-025-06344-z

Xinyu Qin, Zhihong Guo, Zhangyao Nie, Yizhou Yang, Ruiqi Zhang, Xianbing Wang, Zongying Zhang, Chenggui Han, Ying Wang

{"title":"Characterization of chenopodium trirhavirus 1, a novel tri-segmented virus of the family Rhabdoviridae","authors":"Xinyu Qin, Zhihong Guo, Zhangyao Nie, Yizhou Yang, Ruiqi Zhang, Xianbing Wang, Zongying Zhang, Chenggui Han, Ying Wang","doi":"10.1007/s00705-025-06344-z","DOIUrl":"10.1007/s00705-025-06344-z","url":null,"abstract":"<div>A novel tri-segmented virus, tentatively named \"Chenopodium trirhavirus 1\" (CheTRV1), was identified in Chenopodium album in China using high-throughput sequencing combined with conventional Sanger sequencing after RT-PCR. The genome of CheTRV1 consists of three negative-sense single-stranded RNAs designated as RNA1, RNA2, and RNA3. RNAs 1–3 of CheTRV1 share 57.93–64.41%, 48.16–56.96%, and 38.12–42.22% nucleotide sequence identity with the corresponding segments of previously reported trirhaviruses. The 3′- and 5′-termini of the three segments are similar and exhibit partial inverse complementarity. The ORFs of CheTRV1 are separated by a conserved gene junction region with the consensus motif 3′-AAUUCUUUUGN(N)nUUC- 5′. RNA1 consists of 6749 nucleotides (nt) and encodes a single putative L protein. RNA2 is 4393 nt in length and contains four genes in the order 3′-N-P2-P3-P4-5′. The smaller RNA3 consists of 3897 nt and has three genes in the order 3′-P6-P7-P8-5′. Phylogenetic analysis and pairwise comparisons suggested that CheTRV1 is most closely related to Medicago trirhavirus 1, with 68.82% amino acid sequence identity in the L protein. This is the first report of the complete genome sequence of a tri-segmented rhabdovirus, and it expands our knowledge of rhabdovirus evolution.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurovirulence of Zika virus–encoded proteins 寨卡病毒编码蛋白的神经毒力。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-07 DOI: 10.1007/s00705-025-06338-x

Zi-Hui Ma, Li Xing

引用次数: 0

Molecular characterization of a novel non-segmented double stranded RNA mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea 一种感染植物病原真菌葡萄球孢的新型非节段双链RNA分枝病毒的分子特征。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-06 DOI: 10.1007/s00705-025-06324-3

Shunpei Xie, Yanan Zhang, Guoqing Xing, Qinzhou Ma, Yashuang Guo, Rui Zang, Chao Xu, Haiyan Wu, Haiqiang Li, Meng Zhang

{"title":"Molecular characterization of a novel non-segmented double stranded RNA mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea","authors":"Shunpei Xie, Yanan Zhang, Guoqing Xing, Qinzhou Ma, Yashuang Guo, Rui Zang, Chao Xu, Haiyan Wu, Haiqiang Li, Meng Zhang","doi":"10.1007/s00705-025-06324-3","DOIUrl":"10.1007/s00705-025-06324-3","url":null,"abstract":"<div>Botryosphaeria dothidea is a prevalent pathogen of woody plants with a global distribution. In this study, we identified a novel mycovirus from the B. dothidea strain ZM200473, which we have tentatively designated “Botryosphaeria dothidea non-segmented dsRNA virus” (BdNSRV1). The genome of BdNSRV1 is composed of dsRNA that spans 2,902 base pairs and contains two non-overlapping open reading frames (ORF1 and ORF2). ORF1 encodes a hypothetical protein consisting of 316 amino acids with a molecular weight of 35.0 kDa, and this protein shares similarity to the coat proteins of several mycoviruses. ORF2 encodes a protein of 561 amino acids with a molecular weight of 66.0 kDa, which includes a conserved RNA-dependent RNA polymerase (RdRp). Sequence comparisons and phylogenetic analysis indicated that BdNSRV1 establishes a well-supported independent clade alongside members of the recently established genus Unirnavirus, being most closely related to Lasiodiplodia pseudotheobromae mycovirus 1 (LpMyV1), with 70.74% amino acid sequence identity in the RdRp. Therefore, BdNSRV1 should be classified as a novel non-segmented dsRNA mycovirus of the genus Unirnavirus and is the first characterized non-segmented virus associated with B. dothidea.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the mycovirus Aspergillus sulphureus partitivirus 1 分支病毒硫曲霉局部病毒1的鉴定。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-03 DOI: 10.1007/s00705-025-06333-2

Seiji Buma, Syun-ichi Urayama, Kenji Tomita, Sayoko Oiki, Shigeru Okada, Akihiro Ninomiya

{"title":"Characterization of the mycovirus Aspergillus sulphureus partitivirus 1","authors":"Seiji Buma, Syun-ichi Urayama, Kenji Tomita, Sayoko Oiki, Shigeru Okada, Akihiro Ninomiya","doi":"10.1007/s00705-025-06333-2","DOIUrl":"10.1007/s00705-025-06333-2","url":null,"abstract":"<div>Fungi are frequently infected with viruses called mycoviruses. Some mycoviruses have shown potential as biocontrol agents because they can weaken the virulence of fungal plant pathogens. Intensive research has been conducted on the diversity of viruses infecting plant pathogens and the functions of such viruses. In contrast, the diversity of viruses infecting fungi that are not plant pathogens is less well understood. We previously identified a mycovirus of the family Partitiviridae, Aspergillus sulphureus partitivirus 1 (AsuPV1), from an Aspergillus sulphureus strain isolated from fermented dried bonito. In the present study, we conducted a detailed phylogenetic analysis of AsuPV1 and determined its molecular characteristics. The phylogenetic analysis revealed that AsuPV1 belongs to the proposed subgroup I within the genus Gammapartitivirus. Virus particles of AsuPV1 were isolated by density gradient centrifugation, and molecular analyses indicated that each of its three genome segments is encapsulated in capsids composed of the putative viral coat protein. AsuPV1 is the second virus with a tri-segmented genome to be found in a member of Gammapartitivirus subgroup I, after Penicillium stoloniferum virus F. A phenotypic comparison between the parental A. sulphureus strain and isogenic virus-free isolates indicated that AsuPV1 enhances conidium production in its host. This study provides insight into the diversity and molecular characteristics of partitiviruses with a tri-segmented genome.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0