Archives of Virology最新文献_第7页

Identification and complete genome sequence of honeysuckle-associated cytorhabdovirus in China 中国金银花相关细胞habdovirus的鉴定及全基因组序列分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-20 DOI: 10.1007/s00705-025-06238-0

Jing Yang, Liming Liu, Junhui Li, Zikang Chen, Bin Peng, Yongchao Li

{"title":"Identification and complete genome sequence of honeysuckle-associated cytorhabdovirus in China","authors":"Jing Yang, Liming Liu, Junhui Li, Zikang Chen, Bin Peng, Yongchao Li","doi":"10.1007/s00705-025-06238-0","DOIUrl":"10.1007/s00705-025-06238-0","url":null,"abstract":"<div><p>A putatively novel cytorhabdovirus, tentatively named \"honeysuckle-associated cytorhabdovirus\" (HaCV), was identified by high-throughput sequencing in honeysuckle plants exhibiting chlorosis and mottling symptoms. Five isolates were obtained in this study, all of which were found to have a similar genome structure, ranging in length from 13,891 to 13,930 nucleotides and containing eight open reading frames (ORFs). In addition to ORFs encoding the five canonical proteins N, P, M, G, and L, these isolates had an ORF within the <i>P</i> gene, one between the <i>P</i> and <i>M</i> genes, and one between the <i>G</i> and <i>L</i> genes, encoding the putative proteins P′, P3, and P6, respectively. Although phylogenetic analysis showed that all five isolates were most closely related to triticum alphacytorhabdovirus 1 (TriACRV1), genome sequence comparisons showed that the level of sequence identity of isolate FQ3 to TriACRV1 and the other isolates was below the threshold for species demarcation in the genus <i>Cytorhabdovirus</i> (< 75%), whereas the other four isolates– FQ1, FQ2, FQ4, and FQ5– exhibited greater than 75% sequence identity to TriACRV1. Thus, according to the current criteria, the isolates FQ1, FQ2, FQ4, and FQ5 should be considered isolates of TriACRV1, while isolate FQ3 should be classified as a member of a new species.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-025-06238-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic characterization of a novel sakobuvirus (family Picornaviridae) from a European badger (Meles meles) in Hungary 来自匈牙利欧洲獾（Meles Meles）的一种新sakobuvirus （Picornaviridae科）的基因组特征

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-20 DOI: 10.1007/s00705-025-06234-4

Péter Pankovics, Benigna Balázs, Ákos Boros, Gábor Nagy, Sándor Szekeres, Gábor Reuter

引用次数: 0

Structural basis for broad-spectrum binding of AT-9010 to flaviviral methyltransferases AT-9010与黄病毒甲基转移酶广谱结合的结构基础

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-20 DOI: 10.1007/s00705-025-06227-3

Katerina Krejcova, Evzen Boura

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Complete genome sequence analysis of the novel bacteriophage PA-1 infecting phytopathogenic Pantoea ananatis in Malaysia 马来西亚植物病原菌Pantoea ananatis新型噬菌体PA-1的全基因组序列分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-17 DOI: 10.1007/s00705-025-06239-z

Pritpal Singh Sarmak Singh, Wai Keat Toh, Boon Hoe Lim, Hann Ling Wong, Pek Chin Loh

引用次数: 0

Complete genome sequence of a new virga-like virus identified in Viburnum opulus in China 中国圆荚豆科新发现的一种处女样病毒的全基因组序列

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-15 DOI: 10.1007/s00705-025-06244-2

Caixia Yang, Xue Bai, Lei Yang, Song Zhang, Mengji Cao

{"title":"Complete genome sequence of a new virga-like virus identified in Viburnum opulus in China","authors":"Caixia Yang, Xue Bai, Lei Yang, Song Zhang, Mengji Cao","doi":"10.1007/s00705-025-06244-2","DOIUrl":"10.1007/s00705-025-06244-2","url":null,"abstract":"<div><p>A virga-like virus, provisionally named “Viburnum opulus virus 1” (VoV1), was identified by RNA-seq in a <i>Viburnum opulus</i> plant. The monopartite genome of VoV1 is 12,538 nucleotides (nt) in length, excluding the poly(A) tail, and contains seven open reading frames (ORF1–7). The genome organization most closely resembles those of two unclassified plant-infecting virga-like viruses (rubber tree latent virus 1 and 2), which also have seven ORFs but show sequence similarity to VoV1 in only three ORFs. ORF1 is the largest ORF and is predicted to encode a large protein containing six conserved structural domains, including one for the viral replicase. Putative proteins of ORF3 and ORF4 both contain CP-like motifs, while putative proteins encoded by the other ORFs were not identified. Sequence comparisons showed that VoV1 shared the highest nucleotide and amino acid (aa) sequence similarity in ORF1 with rose latent virus 1 (45.0/28.7 identity). Phylogenetic analysis based on the amino acid sequence of the protein encoded by ORF1 showed that VoV1 grouped with some other virga-like viruses in a distinct clade. Analysis of the sequence of VoV1 suggested that it should be classified as a member of a new species within the family <i>Virgaviridae</i>, for which we propose the name “<i>Virgavirus viburnii</i>”.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a novel polymycovirus infecting the entomopathogenic fungus Metarhizium robertsii 一种感染罗伯特绿僵菌的新型多分枝病毒的鉴定

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-15 DOI: 10.1007/s00705-025-06240-6

Cauê Neves Oliveira, Yam de Sousa Santos, Rafael Reis de Rezende, Poliane Alfenas-Zerbini

引用次数: 0

Molecular evidence for goose astrovirus 2 and goose astrovirus 3 coinfection in goslings with gout 鹅星状病毒2型和鹅星状病毒3型在痛风雏鹅中共同感染的分子证据

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-14 DOI: 10.1007/s00705-025-06232-6

Chen Guo, Jingjing Huang, Xiaoyang Hu, Qiong Li, Yinuo Song, Duo Peng, Kang Ning, Xiaoyan Wang, Dabing Zhang

引用次数: 0

Complete genome analysis of a novel nyamivirus from the leaf beetle Aulacophora lewisii 一种新型叶甲虫纳米病毒的全基因组分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-13 DOI: 10.1007/s00705-025-06246-0

Meng-Nan Chen, Xin-Yi Cai, Zhuang-Xin Ye, Ke-Hui Feng, Peng-Peng Ren, Chuan-Xi Zhang, Jian-Ping Chen, Jun-Min Li, Qianzhuo Mao

{"title":"Complete genome analysis of a novel nyamivirus from the leaf beetle Aulacophora lewisii","authors":"Meng-Nan Chen, Xin-Yi Cai, Zhuang-Xin Ye, Ke-Hui Feng, Peng-Peng Ren, Chuan-Xi Zhang, Jian-Ping Chen, Jun-Min Li, Qianzhuo Mao","doi":"10.1007/s00705-025-06246-0","DOIUrl":"10.1007/s00705-025-06246-0","url":null,"abstract":"<div><p>Leaf beetles of the subfamily Chrysomelinae are not only herbivorous pests but also potential vectors of plant pathogens. Here, we describe a novel negative-sense virus named \"Aulacophora lewisii nyamivirus 1\" (ALNyV1), discovered in a leaf beetle (<i>Aulacophora lewisii</i>), a prevalent pest of cucurbitaceous vegetables. Using transcriptome sequencing and rapid amplification of cDNA ends (RACE) techniques, the complete genome sequence of ALNyV1, spanning 10,339 nucleotides (excluding the polyA tail), was determined. A homology search and phylogenetic analysis indicated that ALNyV1 belongs to an unclassified clade in the family <i>Nyamiviridae</i>. This virus contains five open reading frames (ORFs) with typical conserved domains of nyamiviruses. The mean coverage of the ALNyV1 genome was 249, suggesting that active replication had occurred within the leaf beetle host. Furthermore, the virus was found to trigger a small-RNA interference response, with virus-derived small RNAs (vsiRNA) of ALNyV1 displaying a distinct pattern with 21 nucleotides being the most frequent length and exhibiting an A/U bias at the 5' end. This is the first detection of a negative-sense RNA virus in this leaf beetle species, providing important new information about the characteristics of viruses found in leaf beetles.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of a novel narnavirus from the plant-pathogenic fungus Ustilaginoidea virens 植物病原真菌Ustilaginoidea virens中一种新型纳纳病毒的分子特征

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-13 DOI: 10.1007/s00705-025-06241-5

Wenhua Zhao, Zhenrui He, Sisi Liao, Mei Yang, Yingqing Yang, Baoping Cheng, Erxun Zhou

{"title":"Molecular characterization of a novel narnavirus from the plant-pathogenic fungus Ustilaginoidea virens","authors":"Wenhua Zhao, Zhenrui He, Sisi Liao, Mei Yang, Yingqing Yang, Baoping Cheng, Erxun Zhou","doi":"10.1007/s00705-025-06241-5","DOIUrl":"10.1007/s00705-025-06241-5","url":null,"abstract":"<div><p>Mycoviruses are viruses that infect fungi, yeasts, and oomycetes and can replicate and multiply within them. They are widely distributed in plant- and animal-pathogenic fungi. In this study, we identified a novel positive-sense single-stranded RNA (+ ssRNA) mycovirus from <i>Ustilaginoidea virens</i> strain Uv339, the causal agent of rice false smut (RSF), and this virus was named \"Ustilaginoidea virens narnavirus 5\" (UvNV5). Sequence analysis revealed that UvNV5 has a complete genome length of 2091 nt and contains a single open reading frame (ORF) (nt 131–2038) encoding a 635-amino-acid (aa) RNA-dependent RNA polymerase (RdRp) with a molecular mass of 71.8 kDa. BLASTp analysis revealed that the RdRp of UvNV5 shares only 38.22% amino acid sequence identity with that of Ustilaginoidea virens narnavirus virus 13, its closest relative. Phylogenetic analysis indicated that UvNV5 clustered within a branch alongside members of the class <i>Amabiliviricetes</i>. In summary, UvNV5 is a novel mycovirus of the class <i>Amabiliviricetes</i> that infects <i>U. virens</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel SIV-infection animal model based on nude mice inoculated with MT-2 cells 以裸鼠接种MT-2细胞为基础建立新型siv感染动物模型

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-02-13 DOI: 10.1007/s00705-025-06247-z

Lei Xie, Junlin Li, Xiuyun Chen, Mingyu Yin, Wenguan Huang, Minyi Luo, Yatian Liu, Haolan He, Xinyu Zhang, Jinyang He

{"title":"A novel SIV-infection animal model based on nude mice inoculated with MT-2 cells","authors":"Lei Xie, Junlin Li, Xiuyun Chen, Mingyu Yin, Wenguan Huang, Minyi Luo, Yatian Liu, Haolan He, Xinyu Zhang, Jinyang He","doi":"10.1007/s00705-025-06247-z","DOIUrl":"10.1007/s00705-025-06247-z","url":null,"abstract":"<div><p>To establish a novel murine model conducive to simian immunodeficiency virus (SIV) replication <i>in vivo</i>, male BALB/c nude mice, aged three weeks, were administered a single intraperitoneal inoculation of SIV-infected MT-2 cells (derived from human T-cell leukemia). Subsequently, alterations in plasma viral load and the engraftment of MT-2 cells <i>in vivo</i> were examined, while immunological changes were assessed via ELISA and flow cytometry analysis. All of the mice in the experimental group exhibited detectable plasma viral loads in their peripheral circulation, largely due to the proliferation of SIV-infected MT-2 cells within the T-cell-deficient environment of nude mice, resulting in colonization of the abdominal cavity and lymph nodes and the subsequent release of free virions to perpetuate the infection. Mice that received highly active anti-retroviral therapy (HAART) treatment demonstrated a statistically significant reduction in plasma viral loads, with HAART administration partially reversing the course of SIV-induced immune dysfunction. Therefore, the model presented in this study offers substantial potential as a robust tool for evaluating antiviral efficacy and immune modulation in an <i>in vivo</i> setting.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0