Archives of Virology最新文献_第8页

Genomic characteristics of a novel non-segmented double-stranded RNA mycovirus from the fungus Nigrospora oryzae 真菌 Nigrospora oryzae 中一种新型非片段双链 RNA 真菌病毒的基因组特征。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-19 DOI: 10.1007/s00705-024-06178-1

Ya Rong Wang, Jie Zhong, Tian Bo Liu, Yan Song Xiao

引用次数: 0

Association of IL1RN VNTR and NKG2A polymorphisms with hepatitis E infection, a case study from western India IL1RN VNTR 和 NKG2A 多态性与戊型肝炎感染的关系，一项来自印度西部的病例研究。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-19 DOI: 10.1007/s00705-024-06179-0

Anuradha S. Tripathy, Priyanka Wagh, Gajendra Shahapure, Atul M. Walimbe, Nalini Kadgi, Leena Nakate

{"title":"Association of IL1RN VNTR and NKG2A polymorphisms with hepatitis E infection, a case study from western India","authors":"Anuradha S. Tripathy, Priyanka Wagh, Gajendra Shahapure, Atul M. Walimbe, Nalini Kadgi, Leena Nakate","doi":"10.1007/s00705-024-06179-0","DOIUrl":"10.1007/s00705-024-06179-0","url":null,"abstract":"<div><p>Interleukin 1 receptor antagonist (IL1RN) is a competitive inhibitor of interleukin 1 (IL-1). Natural killer cells (NK cells) contribute to the elimination of viruses by their antiviral effector function, which depends on a balance between inhibitory and activating receptor genes such as NKG2D and NKG2A. Using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays, the association of intronic single-nucleotide polymorphisms (SNPs) in these genes with viral infection were assessed in 111 patients with hepatitis E virus (HEV) infection and 222 HEV-naive healthy controls. An SNP in the IL1RN (VNTR) gene revealed allele 2 to be associated with protection against HEV infection (IL1RN *1/*1 vs. IL1RN *2/*2, OR = 0.26, 95% CI = 0.14–0.47, <i>p</i> < 0.001). Similarly, a polymorphism in the intronic region of NKG2A revealed an association with protection in a co-dominant model (A/A vs. A/G: OR = 0.40; 95% CI = 0.24–0.67; A/A vs. G/G: OR = 0.25; 95% CI = 0.10–0.57; <i>p</i> < 0.05) and an association with susceptibility in a dominant model (A/A + A/G vs. G/G: OR = 2.28; 95% CI = 1.06–4.93; <i>p</i> < 0.05) and a recessive model (AA vs. AG + GG: OR = 2.71; 95% CI = 1.66–4.48; <i>p</i> < 0.001). Our data suggest that genetic polymorphisms in host NKG2A and IL1RN have both protective and detrimental roles in HEV infection, although their impact on disease outcome remains unknown.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing a simple and efficient phage biocontainment system using the amber suppressor initiator tRNA 利用琥珀抑制因子启动子 tRNA 设计简单高效的噬菌体生物封闭系统。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-18 DOI: 10.1007/s00705-024-06170-9

Pamela R. Tsoumbris, Russel M. Vincent, Paul R. Jaschke

{"title":"Designing a simple and efficient phage biocontainment system using the amber suppressor initiator tRNA","authors":"Pamela R. Tsoumbris, Russel M. Vincent, Paul R. Jaschke","doi":"10.1007/s00705-024-06170-9","DOIUrl":"10.1007/s00705-024-06170-9","url":null,"abstract":"<div><p>Multidrug-resistant infections are becoming increasingly prevalent worldwide. One of the fastest-emerging alternative and adjuvant therapies being proposed is phage therapy. Naturally isolated phages are used in the vast majority of phage therapy treatments today. Engineered phages are being developed to enhance the effectiveness of phage therapy, but concerns over their potential escape remain a salient issue. To address this problem, we designed a biocontained phage system based on conditional replication using amber stop codon suppression. This system can be easily installed on any natural phage with a known genome sequence. To test the system, we individually mutated the start codons of three essential capsid genes in phage φX174 to the amber stop codon (UAG). These phages were able to efficiently infect host cells expressing the amber initiator tRNA, which suppresses the amber stop codon and initiates translation at TAG stop codons. The amber phage mutants were also able to successfully infect host cells and reduce their population on solid agar and liquid culture but could not produce infectious particles in the absence of the amber initiator tRNA or complementing capsid gene. We did not detect any growth-inhibiting effects on <i>E. coli</i> strains known to lack a receptor for φX174 and we showed that engineered phages have a limited propensity for reversion. The approach outlined here may be useful to control engineered phage replication in both the lab and clinic.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arachis mottle-associated virus, a new polerovirus infecting Pinto peanut 花生斑驳相关病毒，一种感染平托花生的新型多角体病毒。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-17 DOI: 10.1007/s00705-024-06180-7

Caterynne Melo Kauffmann, Alessandra de Jesus Boari, Bruno Arcanjo Silva, Ivair José de Morais, Stephanny Barreto dos Santos Cárdenas, Amanda Moraes do Vale Batista, Helena Beatriz da Silva Mota, Paloma de Souza Queiroz, Késsia Fátima Cunha Pantoja, Bruno Rossitto De Marchi, Giselle Mariano Lessa Assis, Renate Krause-Sakate, Tatsuya Nagata

{"title":"Arachis mottle-associated virus, a new polerovirus infecting Pinto peanut","authors":"Caterynne Melo Kauffmann, Alessandra de Jesus Boari, Bruno Arcanjo Silva, Ivair José de Morais, Stephanny Barreto dos Santos Cárdenas, Amanda Moraes do Vale Batista, Helena Beatriz da Silva Mota, Paloma de Souza Queiroz, Késsia Fátima Cunha Pantoja, Bruno Rossitto De Marchi, Giselle Mariano Lessa Assis, Renate Krause-Sakate, Tatsuya Nagata","doi":"10.1007/s00705-024-06180-7","DOIUrl":"10.1007/s00705-024-06180-7","url":null,"abstract":"<div><p>A new polerovirus, named “arachis mottle-associated virus” (ArMoV), was identified by high-throughput sequencing in a Pinto peanut (<i>Arachis pintoi</i>) plant. The genome sequence was confirmed by Sanger sequencing and contains 5775 nucleotides and seven predicted open reading frames (ORFs), showing a typical polerovirus genome structure. All of the proteins encoded by ArMoV showed less than 90% amino acid sequence identity to those of other poleroviruses, the threshold to establish a new species in the genus. Phylogenetic analysis based on P1-P2 fusion protein and coat protein amino acid sequences showed that tobacco polerovirus 1 and chickpea chlorotic stunt virus, respectively, were the most closely related to ArMoV. These data suggest that ArMoV is a member of a new species of the genus <i>Polerovirus</i>, for which the binomial name \"<i>Polerovirus ARMOV</i> \" is proposed.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular epidemiology of herpangina in the subcenter of Beijing, China: a surveillance study during 2021-2022 中国北京城市副中心疱疹病毒的分子流行病学：2021-2022 年期间的监测研究

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-15 DOI: 10.1007/s00705-024-06171-8

Wan-Xue Zhang, Lin Zou, Yan Cui, Yiguo Zhou, Shan-Shan Zhang, Han Yang, Susu Ding, Qin-Yi Ma, Lu Xi, Ran-Ran Zheng, Juan Du, Jianming Zhang, Qing-Bin Lu

引用次数: 0

Complete genome sequence of tsaoko stripe mosaic virus, a novel macluravirus found in Amomum tsaoko tsaoko条纹镶嵌病毒的完整基因组序列，这是一种在Amomum tsaoko中发现的新型巨型病毒

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-15 DOI: 10.1007/s00705-024-06177-2

Xiyu Yu, Xiaodie Zou, Lei Zhang, Lianzhang Wu, Yi Yang, Guodong Li, Jiahong Dong

引用次数: 0

A novel picorna-like virus in the flatworm Stenostomum leucops (Catenulida) 扁形蠕虫 Stenostomum leucops（Catenulida）中的一种新型皮卡病毒。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-15 DOI: 10.1007/s00705-024-06175-4

Marcos Trindade da Rosa, Gabriel da Luz Wallau, Elgion Lucio Silva Loreto

引用次数: 0

Virus susceptibility of a new cell line derived from the muscle of koi (Cyprinus carpio koi) 从锦鲤（Cyprinus carpio koi）肌肉中提取的新细胞系对病毒的敏感性。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-14 DOI: 10.1007/s00705-024-06168-3

Hongli Jing, Lin Mei, Jizhou Lv, Min Zhang, Na Wang, Lipu Xu, Shaoqiang Wu

{"title":"Virus susceptibility of a new cell line derived from the muscle of koi (Cyprinus carpio koi)","authors":"Hongli Jing, Lin Mei, Jizhou Lv, Min Zhang, Na Wang, Lipu Xu, Shaoqiang Wu","doi":"10.1007/s00705-024-06168-3","DOIUrl":"10.1007/s00705-024-06168-3","url":null,"abstract":"<div><p>In this study, a continuous cell line (KM cells) derived from koi (<i>Cyprinus carpio koi</i>) muscle was established and characterized. The KM cells were subcultured for more than 70 passages and showed high viability after long-term cryopreservation. The KM cell line was optimally cultured in medium 199 containing 10% foetal bovine serum at 25°C. A chromosome analysis indicated that the cell line remained diploid, with a mean chromosome count of 100. DNA sequencing and comparative analysis of the 16S rRNA and cytochrome oxidase I gene sequences showed that the KM cell line originated from koi. In transfection experiments using the plasmid pEGFP, KM cells demonstrated a high level of transfection efficiency, suggesting their potential for use in foreign gene expression studies. Inoculation with spring viraemia of carp virus (SVCV) resulted in a substantial cytopathic effect, and the level of production of SVCV in KM cells was higher than that in the epithelioma papulosum cyprinid (EPC) cell line that is normally used to produce the virus. However, no cytopathic effect was observed when these cells were inoculated with koi herpesvirus, carp oedema virus, or grass carp reovirus. These observations suggest that the newly established KM cell line will be a valuable tool for investigating the pathogenesis of infection with spring viraemia of carp virus.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical detection of four emerging canine diarrhea-associated viruses and evolutionary analysis of canine kobuvirus 四种新出现的犬腹泻相关病毒的临床检测和犬科布瓦病毒的进化分析。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-13 DOI: 10.1007/s00705-024-06161-w

Yongle Yu, Yanzhu Yao, Hu Shan, Xianjie Han

{"title":"Clinical detection of four emerging canine diarrhea-associated viruses and evolutionary analysis of canine kobuvirus","authors":"Yongle Yu, Yanzhu Yao, Hu Shan,  Xianjie Han","doi":"10.1007/s00705-024-06161-w","DOIUrl":"10.1007/s00705-024-06161-w","url":null,"abstract":"<div><p>In this study, a multiplex PCR method was developed for the detection of four diarrhea-associated viruses of canines, including canine bocavirus (CBoV), canine circovirus (CCV), torque teno canis virus (TTCV), and canine kobuvirus (CKV). Four pairs of compatible primers, one specific for each virus, were designed based on conserved sequences. After optimization of parameters such as primer concentration and annealing temperature in single and multiple amplifications, four specific fragments were amplified simultaneously with high sensitivity and specificity in one PCR reaction. The fragments amplified were 165 bp (CBoV), 345 bp (CCV), 506 bp (TTCV), and 666 bp (CKV) in length. The sensitivity of this one-step multiplex PCR is about 10 times lower than that of regular singleplex PCR. There was no cross-reaction with the canine pathogens canine parvovirus (CPV), canine distemper virus (CDV), or canine coronavirus (CCoV). Testing of canine fecal samples from China using the multiplex PCR assay revealed the presence of CBoV, CCV, TTCV, and CKV in 10.1%, 6.2%, 2.8%, and 1.7% of the samples, respectively. The results of multiplex PCR agreed with the singleplex PCR results with a coincidence rate of 100%. In addition, the complete genome sequences of the viruses in three CKV-positive samples were determined and found to be 95.7 − 96.6% identical to the reference strain US-PC0082 and genetically more distant from other animal kobuvirus. The multiplex PCR method established in this study is convenient, with high specificity and sensitivity, which will be helpful for the rapid differential diagnosis of CBoV, CCV, TTCV, and CKV infections.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of antibodies against Epstein–Barr virus nuclear antigen 1 for detection of cellular proteins with monomethylated arginine residues that are potentially involved in viral transformation 使用针对 Epstein-Barr 病毒核抗原 1 的抗体检测可能参与病毒转化的具有单甲基化精氨酸残基的细胞蛋白质

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-08 DOI: 10.1007/s00705-024-06172-7

Christian Graesser, Ruth Nord, Heinrich Flaswinkel, Elisabeth Kremmer, Eckart Meese, Karolina Magdalena Caban, Thomas Fröhlich, Friedrich A. Grässer, Martin Hart

{"title":"Use of antibodies against Epstein–Barr virus nuclear antigen 1 for detection of cellular proteins with monomethylated arginine residues that are potentially involved in viral transformation","authors":"Christian Graesser, Ruth Nord, Heinrich Flaswinkel, Elisabeth Kremmer, Eckart Meese, Karolina Magdalena Caban, Thomas Fröhlich, Friedrich A. Grässer, Martin Hart","doi":"10.1007/s00705-024-06172-7","DOIUrl":"10.1007/s00705-024-06172-7","url":null,"abstract":"<div><p>Epstein–Barr virus nuclear antigen 1 (EBNA1) contains two arginine-glycine (RG) repeats that contain symmetric/asymmetric dimethylarginine (SDMA/ADMA) and monomethylarginine (MMA) residues. We generated mouse monoclonal antibodies directed against a monomethylated GRGRGG-containing repeat located between amino acids 328 and 377 of EBNA1. In addition to detecting MMA-modified EBNA1, we also had the goal of identifying cellular proteins that bind to MMA-modified EBNA1 in EBV-positive Raji cells. Furthermore, we hypothesized that antibodies against MMA-modified EBNA1 might also recognize cell factors that use an MMA-modified surface structure similar to that of EBNA1 to bind to their common targets. Using a combination of immunoprecipitation and mass spectrometry, we identified a number of such cellular proteins, including SNRPD1-3, ALY/REF, RPS15, DIDO1, LSM12, LSM14A, DAP3, and CPSF1. An NACA complex protein that was shown previously to bind to the glycine-alanine repeat of EBNA1 was also identified. The proteins identified in this study are involved in splicing, tumorigenesis, transcriptional activation, DNA stability, and RNA processing or export.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-024-06172-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142596050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0