Archives of Virology最新文献_第10页

miR-126a-5p inhibits H1N1-induced inflammation and matrix protease secretion in lung fibroblasts by targeting ADAMTS-4 miR-126a-5p 通过靶向 ADAMTS-4 抑制 H1N1 引起的肺成纤维细胞炎症和基质蛋白酶分泌。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-11 DOI: 10.1007/s00705-024-06086-4

Fang Fang, Borong Wang, Xiang Lu, Li Wang, Xiangjun Chen, Guanghui Wang, Yifan Yang

{"title":"miR-126a-5p inhibits H1N1-induced inflammation and matrix protease secretion in lung fibroblasts by targeting ADAMTS-4","authors":"Fang Fang, Borong Wang, Xiang Lu, Li Wang, Xiangjun Chen, Guanghui Wang, Yifan Yang","doi":"10.1007/s00705-024-06086-4","DOIUrl":"10.1007/s00705-024-06086-4","url":null,"abstract":"<div><p>Upregulation of ADAMTS-4 has been reported to have an important role in lung injury, and ADAMTS-4 expression is regulated by miR-126a-5p in abdominal aortic aneurysms. The aim of this study was to investigate whether miR-126a-5p/ADAMTS-4 plays a role in influenza-virus-induced lung injury. Lung fibroblasts were infected with H1N1 influenza virus to detect changes in miR-126a-5p and ADAMTS-4 expression, and cell viability was measured by CCK-8 assay. Inflammatory factors and matrix protease levels were examined using ELISA kits, and cell apoptosis was assessed by measuring the levels of apoptosis-related proteins. A dual luciferase assay was used to verify the regulatory relationship between miR-126a-5p and ADAMTS-4. H1N1 influenza virus reduced fibroblast viability, inhibited miR-126a-5p expression, and promoted ADAMTS-4 expression. Overexpression of miR-126a-5p attenuated the cellular inflammatory response, apoptosis, matrix protease secretion, and virus replication. Luciferase reporter assays revealed that miR-126a-5p inhibited ADAMTS-4 expression by targeting ADAMTS-4 mRNA. Further experiments showed that overexpression of ADAMTS-4 significantly reversed the inhibitory effects of miR-126a-5p on fibroblast inflammation, apoptosis, matrix protease secretion, and virus replication. Upregulation of miR-126a-5p inhibits H1N1-induced apoptosis, inflammatory factors, and matrix protease secretion, as well as virus replication in lung fibroblasts.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new geminialphasatellite associated with wheat dwarf virus identified in winter barley in France 在法国冬大麦中发现与小麦矮缩病毒有关的一种新的geminialphasatellite。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-10 DOI: 10.1007/s00705-024-06090-8

Aijun Huang, Laurence Svanella-Dumas, C. Vitry, Armelle Marais, Chantal Faure, Thierry Candresse

{"title":"A new geminialphasatellite associated with wheat dwarf virus identified in winter barley in France","authors":"Aijun Huang, Laurence Svanella-Dumas, C. Vitry, Armelle Marais, Chantal Faure, Thierry Candresse","doi":"10.1007/s00705-024-06090-8","DOIUrl":"10.1007/s00705-024-06090-8","url":null,"abstract":"<div><p>Using a high-throughput sequencing (HTS) approach, we report the discovery of a new alphasatellite identified in a winter barley plant collected in France in 2022 that was also infected by wheat dwarf virus (WDV). The presence of the satellite and of WDV was confirmed by several independent PCR assays, and the complete genome sequence was determined. The circular satellite genome is 1424 nt long and shows typical hallmarks of members of the subfamily <i>Geminialphasatellitinae</i>, including a replication-associated hairpin with a CAGTATTAC sequence and a Rep-encoding open reading frame (ORF). It also possesses a second ORF, embedded in a different frame within the Rep ORF, which is also observed in clecrusatellites and a few other members of the family <i>Alphasatellitidae</i>. Pairwise sequence comparisons and phylogenetic analysis showed that this satellite represents a novel species. Its closest relatives are in the genus <i>Colecusatellite</i>, but it likely represents a new genus given its divergence from other genera of the subfamily <i>Geminialphasatellitinae</i>. Given that WDV was the only virus observed in coinfection with the satellite, the name \"wheat dwarf virus-associated alphasatellite\" is proposed for this novel agent.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome sequence of a novel botourmiavirus infecting the fungus Phomopsis asparagi 感染拟南芥真菌的新型植物病毒的完整基因组序列。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-09 DOI: 10.1007/s00705-024-06084-6

Jingyi Zhou, Shuang Liu, Yun Xu, Junhan Yan, Changping Xie, Li Zheng, Daipeng Chen

引用次数: 0

Complete genome sequence of a novel amalgavirus in sponge gourd, Luffa cylindrica 海绵葫芦（Luffa cylindrica）中一种新型汞病毒的完整基因组序列。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-09 DOI: 10.1007/s00705-024-06094-4

Ting Li, Zhuang-Xin Ye, Yao-Liang Huang, Jian-Ping Chen, Chuan-Xi Zhang, Jun-Min Li

引用次数: 0

A novel betapartitivirus isolated from Cordyceps militaris, an edible-medicinal mushroom 从食用药用菌冬虫夏草中分离出一种新型倍他病毒。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-08 DOI: 10.1007/s00705-024-06085-5

Hanwen Lu, Ping Wang, Jing Sun, Ying Yin, Guogen Yang, Bo Huang

引用次数: 0

Human cytomegalovirus microRNAs: strategies for immune evasion and viral latency 人类巨细胞病毒 microRNAs：免疫逃避和病毒潜伏策略。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-06 DOI: 10.1007/s00705-024-06080-w

Mohammad Sabbaghian, Hamidreza Gheitasi, Manouchehr Fadaee, Helia Javadi Henafard, Ahmad Tavakoli, Ali Akbar Shekarchi, Vahdat Poortahmasebi

{"title":"Human cytomegalovirus microRNAs: strategies for immune evasion and viral latency","authors":"Mohammad Sabbaghian, Hamidreza Gheitasi, Manouchehr Fadaee, Helia Javadi Henafard, Ahmad Tavakoli, Ali Akbar Shekarchi, Vahdat Poortahmasebi","doi":"10.1007/s00705-024-06080-w","DOIUrl":"10.1007/s00705-024-06080-w","url":null,"abstract":"<div><p>Viruses use various strategies and mechanisms to deal with cells and proteins of the immune system that form a barrier against infection. One of these mechanisms is the encoding and production of viral microRNAs (miRNAs), whose function is to regulate the gene expression of the host cell and the virus, thus creating a suitable environment for survival and spreading viral infection. miRNAs are short, single-stranded, non-coding RNA molecules that can regulate the expression of host and viral proteins, and due to their non-immunogenic nature, they are not eliminated by the cells of the immune system. More than half of the viral miRNAs are encoded and produced by <i>Orthoherpesviridae</i> family members. Human cytomegalovirus (HCMV) produces miRNAs that mediate various processes in infected cells to contribute to HCMV pathogenicity, including immune escape, viral latency, and cell apoptosis. Here, we discuss which cellular and viral proteins or cellular pathways and processes these mysterious molecules target to evade immunity and support viral latency in infected cells. We also discuss current evidence that their function of bypassing the host’s innate and adaptive immune system is essential for the survival and multiplication of the virus and the spread of HCMV infection.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141537446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotypic characterization of novel S-DEL variants of porcine epidemic diarrhea virus identified in South Korea 韩国发现的猪流行性腹泻病毒新型 S-DEL 变异株的基因型特征。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-06 DOI: 10.1007/s00705-024-06088-2

Duri Lee, Sungrae Kim, Yunhee Gim, Changhee Lee

{"title":"Genotypic characterization of novel S-DEL variants of porcine epidemic diarrhea virus identified in South Korea","authors":"Duri Lee, Sungrae Kim, Yunhee Gim, Changhee Lee","doi":"10.1007/s00705-024-06088-2","DOIUrl":"10.1007/s00705-024-06088-2","url":null,"abstract":"<div><p>The highly pathogenic genotype 2b (HP-G2b) of porcine epidemic diarrhea virus (PEDV), which caused a pandemic in 2013–2014, evolved in South Korea and became endemic, affecting the domestic pig industry. This study describes the genotypic traits of novel HP-G2b PEDV strains identified on affected farms experiencing low disease severity with < 10% neonatal mortality. Nucleotide sequencing revealed common deletion patterns, termed S-DEL2, resulting in a two-amino-acid deletion at positions 60 and 61, 61 and 62, or 63 and 64 in the N-terminal domain of the spike (S) protein of all isolates. The S barcode profiles of S-DEL2 variants differed from each other and shared 96.0–99.4% and 98.5–99.6% nt sequence identity with other South Korean HP-G2b PEDV strains in the S gene and in the complete genome sequence, respectively. Genetic and phylogenetic analysis showed that the S-DEL2 strains belonged to diverse domestic clades: CK, CK.1, CK.2, or NC. The emergence of novel S-DEL2 strains suggests that continuous evolution of PEDV occurs under endemic circumstances, resulting in genetic diversity and distinct clinical presentations. This study advances our knowledge regarding the genetic and pathogenic heterogeneity of PEDV and emphasizes the importance of active monitoring and surveillance to identify novel variants and determine their genotypic and phenotypic characteristics.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141544503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three novel Enterobacter cloacae bacteriophages for therapeutic use from Ghanaian natural waters 来自加纳天然水域的三种可用于治疗的新型泄殖腔肠杆菌噬菌体。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-05 DOI: 10.1007/s00705-024-06081-9

O. L. Lyytinen, C. Dapuliga, D. Wallinger, S. Patpatia, B. J. Audu, S. J. Kiljunen

{"title":"Three novel Enterobacter cloacae bacteriophages for therapeutic use from Ghanaian natural waters","authors":"O. L. Lyytinen, C. Dapuliga, D. Wallinger, S. Patpatia, B. J. Audu, S. J. Kiljunen","doi":"10.1007/s00705-024-06081-9","DOIUrl":"10.1007/s00705-024-06081-9","url":null,"abstract":"<div><p>Infections caused by multidrug-resistant (MDR) bacteria are a growing global concern. <i>Enterobacter cloacae</i> complex (ECC) species are particularly adept at developing antibiotic resistance. Phage therapy is proposed as an alternative treatment for pathogens that no longer respond to antibiotics. Unfortunately, ECC phages are understudied when compared to phages of many other bacterial species. In this Ghanaian-Finnish study, we isolated two ECC strains from ready-to-eat food samples and three novel phages from natural waters against these strains. We sequenced the genomic DNA of the novel <i>Enterobacter</i> phages, fGh-Ecl01, fGh-Ecl02, and fGh-Ecl04, and assessed their therapeutic potential. All of the phages were found to be lytic, easy to propagate, and lacking any toxic, integrase, or antibiotic resistance genes and were thus considered suitable for therapy purposes. They all were found to be related to T4-type viruses: fGh-Ecl01 and fGh-Ecl04 to karamviruses and fGh-Ecl02 to agtreviruses. Testing of Finnish clinical ECC strains showed promising susceptibility to these novel phages. As many as 61.1% of the strains were susceptible to fGh-Ecl01 and fGh-Ecl04, and 7.4% were susceptible to fGh-Ecl02. Finally, we investigated the susceptibility of the newly isolated ECC strains to three antibiotics – meropenem, ciprofloxacin, and cefepime – in combination with the novel phages. The use of phages and antibiotics together had synergistic effects. When using an antibiotic-phage combination, even low concentrations of antibiotics fully inhibited the growth of bacteria.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11226500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141533391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombination of variable and host range regions of glycoprotein gp85 in different avian leukosis virus subgroup K isolates 不同禽白血病病毒 K 亚群分离株中糖蛋白 gp85 的可变区和宿主范围区的重组。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-06-29 DOI: 10.1007/s00705-024-06083-7

Xiyue Li, Yajun Wang, Jiufeng Li, Zuhua Yu, Ying Wei, Songbiao Chen, Lei He, Ke Ding, Jian Chen

引用次数: 0

Correction: Molecular characterization of lymphocystis disease virus in Indian glass fish: first report from the Andaman Islands 更正：印度玻璃鱼淋巴囊肿病病毒的分子特征：安达曼群岛的首次报告。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-06-27 DOI: 10.1007/s00705-024-06078-4

J. Praveenraj, K. Saravanan, R. Srinath, A. Uma, R. Kiruba Sankar

引用次数: 0