{"title":"Complete genome sequences of two different broad bean stain virus isolates","authors":"Fumino Nito, Yuya Imamura, Yuji Fujiwara, Takayuki Matsuura, Hironobu Yanagisawa","doi":"10.1007/s00705-025-06273-x","DOIUrl":null,"url":null,"abstract":"<div><p>Here, the complete genome sequences of two broad bean stain virus (BBSV; genus <i>Comovirus</i>) strains – Tunisia (RNA1, 5,965 nt; RNA2, 3,467 nt) and Syria (RNA1, 5,976 nt; RNA2, 3,449 nt) – were determined using next-generation sequencing (NGS) and rapid amplification of cDNA ends (RACE). These strains showed 79.2% and 71.6% nucleotide sequence identity to each other in RNA1 and RNA2, respectively. The predicted amino acid sequence identity was 95.2% in the proteinase-polymerase and 93.9% in the coat protein, indicating that these strains belong to the same species in the genus <i>Comovirus</i>. Phylogenetic analysis showed that both strains are closely related to red clover mottle virus (genus <i>Comovirus</i>). This is the first report of the complete genome sequences of two BBSV strains.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 5","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Virology","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s00705-025-06273-x","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Here, the complete genome sequences of two broad bean stain virus (BBSV; genus Comovirus) strains – Tunisia (RNA1, 5,965 nt; RNA2, 3,467 nt) and Syria (RNA1, 5,976 nt; RNA2, 3,449 nt) – were determined using next-generation sequencing (NGS) and rapid amplification of cDNA ends (RACE). These strains showed 79.2% and 71.6% nucleotide sequence identity to each other in RNA1 and RNA2, respectively. The predicted amino acid sequence identity was 95.2% in the proteinase-polymerase and 93.9% in the coat protein, indicating that these strains belong to the same species in the genus Comovirus. Phylogenetic analysis showed that both strains are closely related to red clover mottle virus (genus Comovirus). This is the first report of the complete genome sequences of two BBSV strains.
期刊介绍：
Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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