Archives of Virology最新文献

Molecular characterization of infectious laryngotracheitis virus in China and host transcriptomic responses to immunization with a live attenuated vaccine 中国传染性喉气管炎病毒分子特征及减毒活疫苗免疫后宿主转录组反应

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-30 DOI: 10.1007/s00705-025-06423-1

Xingyang Cui, Shuaiwei Wang, Qingsong Han, Nanying Jin, Huixia Li, Xingya Sun, Yanli Zhao, Houqiang Luo, Yongli Jian, Longchuan Duan

{"title":"Molecular characterization of infectious laryngotracheitis virus in China and host transcriptomic responses to immunization with a live attenuated vaccine","authors":"Xingyang Cui, Shuaiwei Wang, Qingsong Han, Nanying Jin, Huixia Li, Xingya Sun, Yanli Zhao, Houqiang Luo, Yongli Jian, Longchuan Duan","doi":"10.1007/s00705-025-06423-1","DOIUrl":"10.1007/s00705-025-06423-1","url":null,"abstract":"<div><p>Infectious laryngotracheitis (ILT) is a respiratory disorder that poses a global threat to poultry production. It is caused by infectious laryngotracheitis virus (ILTV), a member of the family <i>Orthoherpesviridae.</i> The pathogenicity of different strains of ILTV in intensive poultry operations varies among regions, and the local prevalence of individual strains affects their prioritization in biosafety strategies. Vaccination is the primary method used for controlling ILT. In this study, we sequenced selected genes of ILTV isolates from Zhejiang, China, and analyzed the transcriptome to evaluate the response to immunization of chickens with an attenuated inactivated vaccine. We identified two specific strains of ILTV and compared the sequences of their immune- and virulence-related genes. The results indicated that multiple specific amino acid mutations were located in glycoprotein B, glycoprotein D, and thymidine kinase. The transcriptome of the spleens of chickens that were immunized with a live attenuated vaccine was analyzed, and the immunized group was found to have 33 upregulated and 79 downregulated genes compared with the control group. The differentially expressed genes related to immunity were mainly enriched in the intestinal immune network. The findings of this study provide insights into the genetic diversity of ILTV in Zhejiang and also suggest target genes and pathways that are affected by immunization, providing new information that will facilitate further research on preventing and controlling avian diseases and contribute to our understanding of the molecular mechanisms underlying the immune responses of poultry.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic characterization of rat hepatitis E virus (Rocahepevirus ratti) in urban brown rats (Rattus norvegicus) in Helsinki, Finland 芬兰赫尔辛基城市褐鼠（Rattus norvegicus）中大鼠戊型肝炎病毒（rocahepervirus ratti）的遗传特征

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-29 DOI: 10.1007/s00705-025-06412-4

Mert Erdin, Eda Altan, Nina Suomalainen, Hussein Alburkat, Tuomas Aivelo, Tarja Sironen, Teemu Smura

引用次数: 0

Complete genome sequence of a putative new idaeovirus identified in Cynanchum rostellatum in China 中国一株疑似新冠病毒的全基因组序列分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-27 DOI: 10.1007/s00705-025-06418-y

Xue Bai, Caixia Yang, Jing Wang, Song Zhang, Mengji Cao

{"title":"Complete genome sequence of a putative new idaeovirus identified in Cynanchum rostellatum in China","authors":"Xue Bai, Caixia Yang, Jing Wang, Song Zhang, Mengji Cao","doi":"10.1007/s00705-025-06418-y","DOIUrl":"10.1007/s00705-025-06418-y","url":null,"abstract":"<div><p> A new bipartite idaeovirus tentatively named \"Cynanchum rostellatum idaeovirus 1\" (CRIV-1) was identified in<i>Cynanchum rostellatum</i> exhibiting yellow spots and yellow mosaic symptoms in China. CRIV-1 RNA-1 is 5,418 nucleotides (nt) in length and encodes a polyprotein containing methyltransferase (MTR), helicase (Hel), and RNA-dependent RNA polymerase (RdRp) domains. RNA-2 is 2,224 nt in length and encodes a putative movement protein (MP) and a coat protein (CP). CRIV-1 was found to share the highest nt/amino acid (aa) sequence identity with raspberry bushy dwarf virus (AB948214) in the polyprotein (61.2%/61.8%), with black mulberry idaeovirus (MW017549) in the CP (57.3%/47.4%), and with Actinidia yellowing virus 3 (OL581736) in the MP (59.4%/57.6%). Phylogenetic analysis based on polyprotein aa sequences showed a close relationship of CRIV-1 to other idaeoviruses. CRIV-1 can be transmitted to <i>C. rostellatum </i>and<i>Nicotiana benthamiana</i> plants via mechanical inoculation. Collectively, our data support the classification of CRIV-1 as a new member of the genus <i>Idaeovirus</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145170659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and complete genome sequences of two novel double-stranded RNA viruses infecting the basal fungus Conidiobolus lii 侵染生孢子真菌的两种新型双链RNA病毒的鉴定及全基因组序列分析。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-26 DOI: 10.1007/s00705-025-06413-3

Shaoxiong Zhu, Ping Wang, Ming Yin, Ying Yin, Guogen Yang, Bo Huang

{"title":"Identification and complete genome sequences of two novel double-stranded RNA viruses infecting the basal fungus Conidiobolus lii","authors":"Shaoxiong Zhu, Ping Wang, Ming Yin, Ying Yin, Guogen Yang, Bo Huang","doi":"10.1007/s00705-025-06413-3","DOIUrl":"10.1007/s00705-025-06413-3","url":null,"abstract":"<div><p>Here, we present the identification and complete genome sequences of two novel double-stranded RNA (dsRNA) viruses coinfecting the basal fungus <i>Conidiobolus lii</i> isolate RCEF7535. The viruses, named Conidiobolus lii ootivirus 1 (ClOV1) and Conidiobolus lii totivirus 1 (ClTV1), exhibit distinct genomic characteristics. The genome of ClOV1 is 5,909 nucleotides in length and contains two open reading frames (ORFs). ORF1 encodes a 724-amino-acid capsid protein (CP) with a molecular weight of 80.80 kDa that shows 25.11% sequence identity to the CP of Conidiobolus lamprauges totivirus 1. ORF2 encodes a 743-amino-acid RNA-dependent RNA polymerase (RdRp) with a molecular weight of 83.22 kDa that shares 42.03% sequence identity with the RdRp of Conidiobolus chlamydosporus totivirus 2. The genome of ClTV1 is 4,653 nucleotides in length and contains two ORFs, with ORF1 encoding a 690-amino-acid hypothetical protein (HP, 78.18 kDa) and ORF2 encoding a 799-amino-acid RdRp (90.95 kDa). Both ORFs of ClTV1 display high sequence similarity to hypothetical proteins of Wuhan insect virus 27, with 70.25% identity in the HP and 68.76% identity in the RdRp. Phylogenetic analysis based on RdRp sequences showed that ClOV1 grouped with viruses of the genus <i>Ootivirus</i> within the family <i>Ootiviridae</i>, while ClTV1 clustered with viruses of the genus <i>Totivirus</i> of the family <i>Orthototiviridae</i>. This discovery enhances our understanding of mycovirus diversity, particularly in basal fungi, by identifying novel viruses infecting members of the genus <i>Conidiobolus</i>. </p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome sequence and biological characteristics of a novel duck enteritis virus variant with a deletion in UL2 一种UL2缺失鸭肠炎病毒新变体的全基因组序列和生物学特性

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-24 DOI: 10.1007/s00705-025-06408-0

Huan Yang, Bing Zhang, Xiaoyue Yang, Siyuan Hao, Yafen Song, Xiulei Cai, Xiaofei Song, Chenghuai Yang

{"title":"Complete genome sequence and biological characteristics of a novel duck enteritis virus variant with a deletion in UL2","authors":"Huan Yang, Bing Zhang, Xiaoyue Yang, Siyuan Hao, Yafen Song, Xiulei Cai, Xiaofei Song, Chenghuai Yang","doi":"10.1007/s00705-025-06408-0","DOIUrl":"10.1007/s00705-025-06408-0","url":null,"abstract":"<div><p>A virulent duck enteritis virus (DEV) isolate, designated DEV JS2024, was obtained from diseased ducks on a routinely vaccinated duck farm in Jiangsu Province. The complete genome sequence of this isolate was determined and found to be 161,600 bp in length. The UL2 of DEV JS2024 is 474 bp in length and was found to be completely identical to that of a commercially available attenuated DEV vaccine (GenBank accession number KF487736). However, it was found to have a continuous 528-bp deletion when compared with the Chinese standard virulent strain (GenBank accession number JQ673560). In addition, there were point mutations in UL32, UL20, UL14, UL7, RS1, and US5. Ducks infected with DEV JS2024 showed characteristic DEV pathology, including clinical manifestations and pathological lesions. The titer of DEV JS2024 in ducks was 10<sup>5</sup> LD<sub>50</sub>/mL. Our findings suggest that the 528-bp deletion in the UL2 gene is likely to have been generated through recombination between an attenuated vaccine strain and a wild-type virulent strain. This is the first report of a probable natural DEV recombination event identified in the field. . </p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coordinated role of RIG-I, MDA5, and TLR7 in the innate immune response to duck hepatitis A virus 3 rig - 1、MDA5和TLR7在鸭甲型肝炎病毒先天免疫应答中的协同作用

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-24 DOI: 10.1007/s00705-025-06384-5

Duo Peng, Xiaoyang Hu, Jingjing Huang, Qiong Li, Yinuo Song, Dabing Zhang

引用次数: 0

Feline leukaemia virus subgrouping using a viral interference assay 猫白血病病毒亚群使用病毒干扰试验

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-23 DOI: 10.1007/s00705-025-06407-1

Dimas Arya Abdillah, Takahiro Hiratsuka, Fumiko Matsuyama, Yuki Hattori, Takehisa Soma, Tetsuya Shimoda, Takashi Kato, Hideo Sakai, Masaharu Hisasue, Ariko Miyake, Kazuo Nishigaki

引用次数: 0

Characterization of a neutralizing monoclonal antibody against type I feline coronavirus with post-adsorption blocking activity 具有吸附后阻断活性的I型猫冠状病毒中和性单克隆抗体的鉴定

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-23 DOI: 10.1007/s00705-025-06405-3

Tomoyoshi Doki, Kazuki Kanasaki, Nobuhisa Hasegawa, Okada Daiki, Tomomi Takano

{"title":"Characterization of a neutralizing monoclonal antibody against type I feline coronavirus with post-adsorption blocking activity","authors":"Tomoyoshi Doki, Kazuki Kanasaki, Nobuhisa Hasegawa, Okada Daiki, Tomomi Takano","doi":"10.1007/s00705-025-06405-3","DOIUrl":"10.1007/s00705-025-06405-3","url":null,"abstract":"<div><p>Feline infectious peritonitis (FIP) is a fatal disease of cats that is caused by feline coronavirus (FCoV). FCoV is classified into two serotypes, with type I FCoV being predominant in natural infections. Despite its clinical significance, there is still a lack of effective preventive and therapeutic interventions for FIP. Neutralizing monoclonal antibodies (mAbs) have proven effective against various viral infections; however, no neutralizing mAbs have been developed for type I FCoV, and their potential therapeutic application for treatment of FIP remains to be explored. In this study, we generated Ya-NT-1, the first mAb capable of neutralizing type I FCoV <i>in vitro</i>, using a conventional hybridoma approach. Ya-NT-1 specifically recognized type I FCoV but did not react with type II FCoV, confirming its serotype specificity. However, western blot analysis failed to identify the viral structural protein targeted by Ya-NT-1, suggesting that it may recognize a conformational epitope. To investigate the mechanism of neutralization, we evaluated the ability of mAb Ya-NT-1 to block viral adsorption under different treatment conditions. Type I FCoV was effectively neutralized when the antibody was administered post-adsorption but not pre-adsorption, indicating that it acts at an early post-entry stage rather than preventing viral attachment. This suggests that Ya-NT-1 might interfere with membrane fusion or genome release, similar to previously described post-entry neutralizing antibodies against other coronaviruses. These findings represent a critical first step toward understanding the neutralization mechanisms of type I FCoV and lay the groundwork for developing antibody-based therapies for FIP. Further studies are needed to determine the precise target of Ya-NT-1 and to assess its therapeutic potential, including strategies to mitigate the risk of antibody-dependent enhancement.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal dynamics and genetic diversity of human rhinoviruses in patients with acute respiratory infection in Bangkok in 2024 2024年曼谷急性呼吸道感染患者鼻病毒的季节性动态和遗传多样性

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-18 DOI: 10.1007/s00705-025-06406-2

Jiratchaya Puenpa, Sovida Dara, Preeyaporn Vichaiwattana, Ratchadawan Aeemjinda, Yong Poovorawan

{"title":"Seasonal dynamics and genetic diversity of human rhinoviruses in patients with acute respiratory infection in Bangkok in 2024","authors":"Jiratchaya Puenpa, Sovida Dara, Preeyaporn Vichaiwattana, Ratchadawan Aeemjinda, Yong Poovorawan","doi":"10.1007/s00705-025-06406-2","DOIUrl":"10.1007/s00705-025-06406-2","url":null,"abstract":"<div><p>Human rhinoviruses (HRVs) are significant contributors to respiratory illnesses, particularly acute respiratory infections (ARIs). In this study, we investigated the prevalence and genotypes of HRV circulating in Bangkok in 2024. Nasopharyngeal swabs were collected from 8,097 patients hospitalized with ARIs, resulting in a 14.5% HRV positivity rate (n = 1,320). Among these, children under 12 years accounted for the largest proportion (54.5%), highlighting a substantial disease burden in this age group. Molecular typing was performed on a subset of 360 HRV-positive samples. HRV-A was the most frequently detected type (64.4%), followed by HRV-C (33.1%) and HRV-B (2.5%). Seasonal variation was observed, with HRV-A predominating during the rainy season. Genotyping revealed diverse circulating strains, with HRV-C42, HRV-A73, and HRV-C32 among the most prevalent genotypes, indicating a shift from previously identified strains. Phylogenetic analysis demonstrated that circulating HRV strains in Bangkok share close genetic relationships with isolates from other countries, suggesting ongoing global transmission dynamics. These findings highlight the evolving landscape of HRV infections and underscore the importance of ongoing surveillance and genetic characterization to monitor circulating strains. This information is crucial for informing public-health strategies aimed at controlling respiratory infections, particularly among vulnerable populations such as children and the elderly.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145073685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of PERK-eIF2α-ATF4 signaling enhances the antiviral effects of resveratrol-loaded nanoparticles against enterovirus 71 in hand, foot, and mouth disease 抑制PERK-eIF2α-ATF4信号可增强白藜芦醇负载纳米颗粒对手足口病肠道病毒71的抗病毒作用

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-16 DOI: 10.1007/s00705-025-06392-5

Bin Wang, Lihe Che, Peng Zhang, Luyao Sun, Yue Yu, Na Du

{"title":"Inhibition of PERK-eIF2α-ATF4 signaling enhances the antiviral effects of resveratrol-loaded nanoparticles against enterovirus 71 in hand, foot, and mouth disease","authors":"Bin Wang, Lihe Che, Peng Zhang, Luyao Sun, Yue Yu, Na Du","doi":"10.1007/s00705-025-06392-5","DOIUrl":"10.1007/s00705-025-06392-5","url":null,"abstract":"<p>Resveratrol-loaded nanoparticles (RES-NPs) have been found to reduce enterovirus 71 (EV71) replication in EV71-infected-rhabdosarcoma (RD) cells. However, the specific mechanism by which RES-NPs prevent EV71 infection in RD cells remains largely unclear. The cell viability, inflammatory response, and oxidative stress in EV71-infected RD cells were assessed. Inhibition of protein kinase RNA-like endoplasmic reticulum kinase (PERK) significantly increased the viability of infected RD cells, reduced inflammation and oxidative stress, and led to a significant decrease in EV71 mRNA levels. Furthermore, treatment of infected RD cells with RES-NPs significantly increased cell viability and alleviated inflammation and oxidative stress, and these effects were further enhanced by inhibition of PERK. RES-NP treatment also resulted in a decrease in phosphorylated PERK, phosphorylated eukaryotic translation initiation factor 2α (eIF2α), and activating transcription factor 4 (ATF4) levels in infected RD cells, and the levels of these protein were further reduced by treatment with the PERK inhibitor. RES-NPs were found to inhibit EV71 infection by reducing virus-induced inflammatory responses and oxidative stress in RD cells, possibly through inactivation of the PERK-eIF2α-ATF4 signaling pathway.</p>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0