Archives of Virology最新文献

Characterization of two Campylobacter jejuni phages and evaluation of their antibacterial efficacy with EDTA 两种空肠弯曲杆菌噬菌体的特征及其与 EDTA 的抗菌效果评估

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-20 DOI: 10.1007/s00705-024-06169-2

Su Zar Chi Lwin, Aye Thida Maung, Khin Zar Linn, Miku Hirono, Cunkuan Shen, Mohamed El-Telbany, Marwa Nabil Sayed Abdelaziz, Tahir Noor Mohammadi, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

{"title":"Characterization of two Campylobacter jejuni phages and evaluation of their antibacterial efficacy with EDTA","authors":"Su Zar Chi Lwin, Aye Thida Maung, Khin Zar Linn, Miku Hirono, Cunkuan Shen, Mohamed El-Telbany, Marwa Nabil Sayed Abdelaziz, Tahir Noor Mohammadi, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto","doi":"10.1007/s00705-024-06169-2","DOIUrl":"10.1007/s00705-024-06169-2","url":null,"abstract":"<div>Campylobacter jejuni is a leading cause of foodborne illness worldwide. The application of bacteriophages offers a promising approach to specifically target and reduce C. jejuni contamination in food products. In this study, two C. jejuni phages were characterized, and their ability to inhibit bacterial growth in combination with ethylenediaminetetraacetic acid (EDTA) was investigated. Both phages exhibited tolerance to a wide range of temperature (4–60 °C) and pH (3-9). Phage vB_CjeM-PC10 and vB_CjeM-PC22 were found to have a latent period of 30 min and 20 min and a burst size of 7 and 35 PFU/cell, respectively. Phage vB_CjeM-PC10 has a linear double-stranded DNA (dsDNA) genome of 51,148 bp with 77 ORFs and 29% GC content. Phage vB_CjeM-PC22 has a circular dsDNA genome of 32,543 bp with 56 ORFs and 28% GC content. At 42 °C, the combination of these phages (MOI = 10) and EDTA decreased the count of viable C. jejuni by 5.2 log10 and inhibited the regrowth of resistant cells for 48 h. At 4 °C, phage vB_CjeM-PC10 alone (MOI = 1000) reduced the count of viable C. jejuni by 3 log10 in brain heart infusion (BHI) broth and 2 log10 on chicken skin after incubation for 48 h. Although these phages were effective against C. jejuni, they cannot be utilized directly for food safety applications because they are lysogenic. Nevertheless, these findings expand the genome library of C. jejuni phages and enrich data resources by highlighting potential strategies for controlling C. jejuni infections.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142672556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can viral proteins be retooled for chimeric toxin development? 病毒蛋白能否重新用于嵌合毒素的开发？

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-20 DOI: 10.1007/s00705-024-06173-6

Caroline Ismeurt-Walmsley, Eric J. Kremer

引用次数: 0

Complete genome sequence of a rare recombinant GII.5[P16] norovirus found in Russian Siberia 俄罗斯西伯利亚发现的罕见重组 GII.5[P16]诺如病毒的完整基因组序列

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-19 DOI: 10.1007/s00705-024-06176-3

E. Zhirakovskaia, A. Tikunov, B. Kravchuk, N. Tikunova

引用次数: 0

Genomic characteristics of a novel non-segmented double-stranded RNA mycovirus from the fungus Nigrospora oryzae 真菌 Nigrospora oryzae 中一种新型非片段双链 RNA 真菌病毒的基因组特征。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-19 DOI: 10.1007/s00705-024-06178-1

Ya Rong Wang, Jie Zhong, Tian Bo Liu, Yan Song Xiao

引用次数: 0

Association of IL1RN VNTR and NKG2A polymorphisms with hepatitis E infection, a case study from western India IL1RN VNTR 和 NKG2A 多态性与戊型肝炎感染的关系，一项来自印度西部的病例研究。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-19 DOI: 10.1007/s00705-024-06179-0

Anuradha S. Tripathy, Priyanka Wagh, Gajendra Shahapure, Atul M. Walimbe, Nalini Kadgi, Leena Nakate

{"title":"Association of IL1RN VNTR and NKG2A polymorphisms with hepatitis E infection, a case study from western India","authors":"Anuradha S. Tripathy, Priyanka Wagh, Gajendra Shahapure, Atul M. Walimbe, Nalini Kadgi, Leena Nakate","doi":"10.1007/s00705-024-06179-0","DOIUrl":"10.1007/s00705-024-06179-0","url":null,"abstract":"<div>Interleukin 1 receptor antagonist (IL1RN) is a competitive inhibitor of interleukin 1 (IL-1). Natural killer cells (NK cells) contribute to the elimination of viruses by their antiviral effector function, which depends on a balance between inhibitory and activating receptor genes such as NKG2D and NKG2A. Using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays, the association of intronic single-nucleotide polymorphisms (SNPs) in these genes with viral infection were assessed in 111 patients with hepatitis E virus (HEV) infection and 222 HEV-naive healthy controls. An SNP in the IL1RN (VNTR) gene revealed allele 2 to be associated with protection against HEV infection (IL1RN *1/*1 vs. IL1RN *2/*2, OR = 0.26, 95% CI = 0.14–0.47, p < 0.001). Similarly, a polymorphism in the intronic region of NKG2A revealed an association with protection in a co-dominant model (A/A vs. A/G: OR = 0.40; 95% CI = 0.24–0.67; A/A vs. G/G: OR = 0.25; 95% CI = 0.10–0.57; p < 0.05) and an association with susceptibility in a dominant model (A/A + A/G vs. G/G: OR = 2.28; 95% CI = 1.06–4.93; p < 0.05) and a recessive model (AA vs. AG + GG: OR = 2.71; 95% CI = 1.66–4.48; p < 0.001). Our data suggest that genetic polymorphisms in host NKG2A and IL1RN have both protective and detrimental roles in HEV infection, although their impact on disease outcome remains unknown.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing a simple and efficient phage biocontainment system using the amber suppressor initiator tRNA 利用琥珀抑制因子启动子 tRNA 设计简单高效的噬菌体生物封闭系统。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-18 DOI: 10.1007/s00705-024-06170-9

Pamela R. Tsoumbris, Russel M. Vincent, Paul R. Jaschke

{"title":"Designing a simple and efficient phage biocontainment system using the amber suppressor initiator tRNA","authors":"Pamela R. Tsoumbris, Russel M. Vincent, Paul R. Jaschke","doi":"10.1007/s00705-024-06170-9","DOIUrl":"10.1007/s00705-024-06170-9","url":null,"abstract":"<div>Multidrug-resistant infections are becoming increasingly prevalent worldwide. One of the fastest-emerging alternative and adjuvant therapies being proposed is phage therapy. Naturally isolated phages are used in the vast majority of phage therapy treatments today. Engineered phages are being developed to enhance the effectiveness of phage therapy, but concerns over their potential escape remain a salient issue. To address this problem, we designed a biocontained phage system based on conditional replication using amber stop codon suppression. This system can be easily installed on any natural phage with a known genome sequence. To test the system, we individually mutated the start codons of three essential capsid genes in phage φX174 to the amber stop codon (UAG). These phages were able to efficiently infect host cells expressing the amber initiator tRNA, which suppresses the amber stop codon and initiates translation at TAG stop codons. The amber phage mutants were also able to successfully infect host cells and reduce their population on solid agar and liquid culture but could not produce infectious particles in the absence of the amber initiator tRNA or complementing capsid gene. We did not detect any growth-inhibiting effects on E. coli strains known to lack a receptor for φX174 and we showed that engineered phages have a limited propensity for reversion. The approach outlined here may be useful to control engineered phage replication in both the lab and clinic.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arachis mottle-associated virus, a new polerovirus infecting Pinto peanut 花生斑驳相关病毒，一种感染平托花生的新型多角体病毒。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-17 DOI: 10.1007/s00705-024-06180-7

Caterynne Melo Kauffmann, Alessandra de Jesus Boari, Bruno Arcanjo Silva, Ivair José de Morais, Stephanny Barreto dos Santos Cárdenas, Amanda Moraes do Vale Batista, Helena Beatriz da Silva Mota, Paloma de Souza Queiroz, Késsia Fátima Cunha Pantoja, Bruno Rossitto De Marchi, Giselle Mariano Lessa Assis, Renate Krause-Sakate, Tatsuya Nagata

{"title":"Arachis mottle-associated virus, a new polerovirus infecting Pinto peanut","authors":"Caterynne Melo Kauffmann, Alessandra de Jesus Boari, Bruno Arcanjo Silva, Ivair José de Morais, Stephanny Barreto dos Santos Cárdenas, Amanda Moraes do Vale Batista, Helena Beatriz da Silva Mota, Paloma de Souza Queiroz, Késsia Fátima Cunha Pantoja, Bruno Rossitto De Marchi, Giselle Mariano Lessa Assis, Renate Krause-Sakate, Tatsuya Nagata","doi":"10.1007/s00705-024-06180-7","DOIUrl":"10.1007/s00705-024-06180-7","url":null,"abstract":"<div>A new polerovirus, named “arachis mottle-associated virus” (ArMoV), was identified by high-throughput sequencing in a Pinto peanut (Arachis pintoi) plant. The genome sequence was confirmed by Sanger sequencing and contains 5775 nucleotides and seven predicted open reading frames (ORFs), showing a typical polerovirus genome structure. All of the proteins encoded by ArMoV showed less than 90% amino acid sequence identity to those of other poleroviruses, the threshold to establish a new species in the genus. Phylogenetic analysis based on P1-P2 fusion protein and coat protein amino acid sequences showed that tobacco polerovirus 1 and chickpea chlorotic stunt virus, respectively, were the most closely related to ArMoV. These data suggest that ArMoV is a member of a new species of the genus Polerovirus, for which the binomial name \"Polerovirus ARMOV \" is proposed.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular epidemiology of herpangina in the subcenter of Beijing, China: a surveillance study during 2021-2022 中国北京城市副中心疱疹病毒的分子流行病学：2021-2022 年期间的监测研究

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-15 DOI: 10.1007/s00705-024-06171-8

Wan-Xue Zhang, Lin Zou, Yan Cui, Yiguo Zhou, Shan-Shan Zhang, Han Yang, Susu Ding, Qin-Yi Ma, Lu Xi, Ran-Ran Zheng, Juan Du, Jianming Zhang, Qing-Bin Lu

引用次数: 0

Complete genome sequence of tsaoko stripe mosaic virus, a novel macluravirus found in Amomum tsaoko tsaoko条纹镶嵌病毒的完整基因组序列，这是一种在Amomum tsaoko中发现的新型巨型病毒

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-15 DOI: 10.1007/s00705-024-06177-2

Xiyu Yu, Xiaodie Zou, Lei Zhang, Lianzhang Wu, Yi Yang, Guodong Li, Jiahong Dong

引用次数: 0

A novel picorna-like virus in the flatworm Stenostomum leucops (Catenulida) 扁形蠕虫 Stenostomum leucops（Catenulida）中的一种新型皮卡病毒。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-11-15 DOI: 10.1007/s00705-024-06175-4

Marcos Trindade da Rosa, Gabriel da Luz Wallau, Elgion Lucio Silva Loreto

引用次数: 0