Archives of Virology最新文献

Environmental variability and pathogen synergy influencing tilapia lake virus outbreaks on tilapia farms in Tamil Nadu, India.

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-24 DOI: 10.1007/s00705-025-06355-w

Alagukanthasami Ponsrinivasan, Arumugam Uma, P Chidambaram, Cheryl Antony, Arun Sudhagar, Sethu Selvaraj, Subash Palaniappan, Manimozhi Ethirajan

引用次数: 0

Identification and complete genome sequence of a new cheravirus discovered in grapevine. 葡萄中一种新冠状病毒的鉴定及全基因组序列分析。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-21 DOI: 10.1007/s00705-025-06340-3

Xudong Fan, Yuxin Hao, Fang Ren, Guojun Hu, Ying Zhang, Qingyun Yuan, Tingting Du, Jie Gao, Meilong Xu, Yafeng Dong

{"title":"Identification and complete genome sequence of a new cheravirus discovered in grapevine.","authors":"Xudong Fan, Yuxin Hao, Fang Ren, Guojun Hu, Ying Zhang, Qingyun Yuan, Tingting Du, Jie Gao, Meilong Xu, Yafeng Dong","doi":"10.1007/s00705-025-06340-3","DOIUrl":"10.1007/s00705-025-06340-3","url":null,"abstract":"High-throughput sequencing (HTS) was applied to identify viruses present in grapevine samples collected in Ningxia, China, that exhibited obvious symptoms of viral disease. A putative novel cheravirus was found through the analysis of sequencing data, and its presence was confirmed by RT-PCR in a Cabernet Sauvignon sample with vein yellowing. The complete genome sequence of this virus was determined by Sanger sequencing of overlapping RT-PCR products and the rapid amplification of cDNA ends (RACE) method. The genome of the putative virus comprises two RNA segments 7,395 and 3,645 nucleotides in length, excluding their poly-A tails. The new virus was tentatively named \"grapevine cheravirus\" (GCheV). The Pro-Pol region of RNA1 and the CP region of RNA2 of GCheV shared the highest amino acid sequence identity, reaching 72.0% and 48.9%, respectively, with the corresponding sequences of Corymbium villosum cheravirus (CvCV). Phylogenetic analysis revealed that GCheV clustered with CvCV in the genus Cheravirus. These results suggest that GCheV is a new member of that genus. To the best of our knowledge, this is the first report of a virus belonging to the genus Cheravirus in grapevines.","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"160"},"PeriodicalIF":2.5,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of a novel negative-sense RNA virus infecting Fusarium commune, a fungus causing tobacco root rot. 一种侵染烟草根腐病镰刀菌的新型负义RNA病毒的鉴定与鉴定。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-21 DOI: 10.1007/s00705-025-06341-2

Huagang Xiao, Yonghui Peng, Kai Teng, Xiangping Zhou, Yansong Xiao, Zhenyu Deng, Qi Huang, Can Wang, Lu Zheng, Jiatao Xie, Tianbo Liu

引用次数: 0

The full‑length genome sequence of a novel polymycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea. 一种感染植物病原真菌马铃薯球孢菌的新型多分枝病毒的全基因组序列。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-17 DOI: 10.1007/s00705-025-06330-5

Shunpei Xie, Yanfen Wang, Fengyue Gong, Haiyan Wu, Haiqiang Li, Qinzhou Ma, Yashuang Guo, Yuehua Geng, Bingshan Liu, Meng Zhang

{"title":"The full‑length genome sequence of a novel polymycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea.","authors":"Shunpei Xie, Yanfen Wang, Fengyue Gong, Haiyan Wu, Haiqiang Li, Qinzhou Ma, Yashuang Guo, Yuehua Geng, Bingshan Liu, Meng Zhang","doi":"10.1007/s00705-025-06330-5","DOIUrl":"https://doi.org/10.1007/s00705-025-06330-5","url":null,"abstract":"Here, a novel dsRNA virus belonging to the family Polymycoviridae was identified in the phytopathogenic fungal strain B. dothidea ZM200473 and tentatively named \"Botryosphaeria dothidea polymycovirus 2\" (BdPmV2). The genome of BdPmV2 consists of five genomic dsRNA segments, ranging in size from 1224 to 2407 bp, each containing an open reading frame (ORF). ORF1 encodes a conserved RNA-dependent RNA polymerase (RdRP) consisting of 763 amino acids (aa) with a molecular mass (Mr) of 84.03 kDa, ORF3 encodes a putative methyltransferase (Met) consisting of 627 amino acids (aa) with an Mr of 68.41 kDa, ORF4 encodes a P-A-S-rich protein that serves as a coat protein (CP) consisting of 261 amino acids (aa) with an Mr of 27.60 kDa. ORF2 and ORF5 encode putative proteins with unknown functions, consisting of 697 amino acids (aa) with an Mr of 76.49 kDa and 323 amino acids (aa) with an Mr of 33.92 kDa, respectively. BLASTp analysis revealed that the RdRP protein of BdPmV2 had the highest similarity (56.52% identity) to that of a virus previously identified as \"Aspergillus fumigatus polymycovirus 1\". Phylogenetic analysis based on RdRP aa sequences indicated that BdPmV2 is a new member of the family Polymycoviridae.","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"159"},"PeriodicalIF":2.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplex quantitative PCR assay for porcine circoviruses 2 and 3 and its application for measuring infection rates at different stages of pig production. 猪圆环病毒2型和3型多重定量PCR检测及其在猪生产不同阶段感染率测定中的应用

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-16 DOI: 10.1007/s00705-025-06351-0

Zhen Li, Shuaiyin Guan, Jiaying Zhao, Yuhuan Chen, Yang Han, Ang Tian, Saisai Zhou, Huanchun Chen, Yunfeng Song

{"title":"Multiplex quantitative PCR assay for porcine circoviruses 2 and 3 and its application for measuring infection rates at different stages of pig production.","authors":"Zhen Li, Shuaiyin Guan, Jiaying Zhao, Yuhuan Chen, Yang Han, Ang Tian, Saisai Zhou, Huanchun Chen, Yunfeng Song","doi":"10.1007/s00705-025-06351-0","DOIUrl":"https://doi.org/10.1007/s00705-025-06351-0","url":null,"abstract":"Porcine circovirus 2 (PCV2) and porcine circovirus 3 (PCV3) are considered a threat to the pig industry due to their association with growth retardation and reproductive disorders. In this study, we developed a multiplex real-time PCR (qPCR) assay for simultaneous detection of PCV2 and PCV3 and used it to investigate the epidemiology of PCV2 and PCV3 at different stages of pig production. The sensitivity of the multiplex qPCR was 4.32 × 101copies/µL for PCV2 and 1.01 × 101copies/µL for PCV3, and the coefficient of variation was less than 1%. The correlation coefficients (R2) of the standard curves were all greater than 0.990. Out of 1241 samples tested, nine (0.73%) were positive for PCV2, 209 (16.84%) for PCV3, and three (0.24%) for both. PCV2 was detected in saliva from asymptomatic gilts on one farm, and PCV3 was detected in all sample types except semen at all production stages on all of the farms where samples were collected. The main sample types that tested positive were saliva (23.19%, 77/322), blood (17.80%, 102/573), saliva/blood mixture (18.75%, 9/48), and pigpen swabs (32.14%, 9/28). Viral loads ranged from 103.9 to 108.2 copies/mL. Gilts (37.85%, 81/214) and grow-finish pigs (42.25%, 30/71) were the main asymptomatic PCV3 carriers. Piglets (16.67%, 3/18) and nursery pigs (22.73%, 5/22) were the main symptomatic PCV3 carriers. The assay described here is a practical and sensitive diagnostic technique for identification and monitoring of PCV2 and PCV3 infections and can provide new information on the epidemiology of PCV2 and PCV3 that can be applied for developing effective prevention and control strategies.","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"158"},"PeriodicalIF":2.5,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological characteristics of human parainfluenza virus infections and phylogenetic analysis of human parainfluenza virus type 3 isolated from children with respiratory tract infections from 2020 to 2022 in Zhejiang, China. 2020 - 2022年浙江省人副流感病毒感染流行病学特征及3型人副流感病毒分离株系统发育分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-13 DOI: 10.1007/s00705-025-06343-0

Yun Lu, Jili Ni, Shuangshuang Huang, Yajun Guo, Yingying Chen, Shufa Zheng, Yingshuo Wang, Wei Li

{"title":"Epidemiological characteristics of human parainfluenza virus infections and phylogenetic analysis of human parainfluenza virus type 3 isolated from children with respiratory tract infections from 2020 to 2022 in Zhejiang, China.","authors":"Yun Lu, Jili Ni, Shuangshuang Huang, Yajun Guo, Yingying Chen, Shufa Zheng, Yingshuo Wang, Wei Li","doi":"10.1007/s00705-025-06343-0","DOIUrl":"https://doi.org/10.1007/s00705-025-06343-0","url":null,"abstract":"Human parainfluenza viruses (HPIVs) are a major cause of pediatric respiratory hospitalizations worldwide. HPIV-3 is one of the most common subtypes of HPIV. In this study, we retrospectively analyzed the respiratory pathogen detection results for 23,775 children with ARIs who were treated in Zhejiang Province from January 2020 to December 2022, 610 HPIVs-positive specimens were selected for parainfluenza virus typing, and 35 HPIV-3-positive samples were selected for HN gene sequencing and analysis. In total, 1383 of the specimens were positive for HPIV, resulting in a detection rate of 5.82%. During this period, the detection rate was the highest in 2020, at 7.77%. The detection rate in autumn was 9.17%, surpassing those in other seasons. Among the different age groups, schoolchildren had the lowest detection rate of 1.75%. HPIV-3 was the predominant subtype, accounting for 76% of HPIV-positive cases. The nucleotide and amino acid sequence identity of the 35 HPIV-3 HN genes that were examined ranged from 97.0-100.0% and 98.6-100.0%, respectively. Phylogenetic analysis revealed that 27 isolates belonged to the C3a subtype and that eight belonged to the C3b subtype. A total of 171 nucleotide variations and 30 amino acid variations were identified among the 35 isolates. These findings confirm that HPIV, especially HPIV-3, was a significant pathogen in acute respiratory infection among pediatric patients in Zhejiang from 2020 to 2022. The observation of high sequence similarity and relatively stable genetic evolution of HPIV-3 in this region provides valuable insights into the molecular and epidemiological characteristics of this virus.","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"157"},"PeriodicalIF":2.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and genetic analysis of two sugarlandviruses: Klebsiella variicola lytic phages vB_PZK-KV7 and vB_PZK-KV23. 两种糖地病毒：水痘克雷伯菌裂解噬菌体vB_PZK-KV7和vB_PZK-KV23的鉴定与遗传分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-12 DOI: 10.1007/s00705-025-06331-4

Le Zhong, Yunqian Sun, Yunhao Sun, Guohui Yu, Ping Cheng, Jinshui Zheng, Donghai Peng, Ming Sun

引用次数: 0

Construction of a shortened autographa californica multiple nucleopolyhedrovirus genome as protein expression vector. 加州签名多核多角体病毒短基因组蛋白表达载体的构建。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-12 DOI: 10.1007/s00705-025-06349-8

Ziqian Cao, Xinyu Liu, Jianchao Li, Yu Zheng, Jun Yin, Hongyu Wang, Xiaoyue Zhang, Hongying Chen

{"title":"Construction of a shortened autographa californica multiple nucleopolyhedrovirus genome as protein expression vector.","authors":"Ziqian Cao, Xinyu Liu, Jianchao Li, Yu Zheng, Jun Yin, Hongyu Wang, Xiaoyue Zhang, Hongying Chen","doi":"10.1007/s00705-025-06349-8","DOIUrl":"https://doi.org/10.1007/s00705-025-06349-8","url":null,"abstract":"The baculovirus expression system (BEVS) is a powerful tool for protein production in insect cells, and the most widely used virus vector in the BEVS is Autographa californica multiple nucleopolyhedrovirus (AcMNPV), whose genome contains 155 open reading frames (ORFs), nearly half of which are nonessential for viral replication in vitro. Our previous studies have shown that deletion of some large nonessential fragments from the AcMNPV genome can significantly improve the efficiency of production of foreign proteins and that the viral vector can be shortened by more than 10 kb. Using a previously described shortened vector, four additional fragments containing multiple nonessential genes (ac58-61, ac110-114, ac116-119, and ac121-124) were removed from the AcMNPV genome, and the effects on baculovirus replication and foreign protein expression were examined. The results showed that deletion of one of the nonessential fragments did not affect virus replication. For the expression of foreign proteins, removal of ac110-114 and ac116-119 was beneficial for protein production, deletion of ac121-124 apparently did not affect protein production, and deletion of ac58-61 severely impaired protein expression. Simultaneous deletion of two fragments from the AcMNPV genome significantly influenced exogenous protein expression and/or virus replication. Finally, by injection into Spodoptera frugiperda larvae, it was shown that the three shortened AcMNPVs that had maintained their infectivity and ability to express foreign proteins in cultured cells also retained their infectivity in vivo. The three shortened baculovirus expression vectors obtained in this study were 127-129 kb in length, representing a further reduction of 1.4-2.3% in genome size compared to the previously shortened vector, further optimizing it for protein production in the BEVS.","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"155"},"PeriodicalIF":2.5,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insertion of the P gene of spring viremia of carp virus into the snakehead rhabdovirus genome increases virulence. 将鲤鱼病毒春季毒血症P基因插入到蛇头横纹肌病毒基因组中，毒力增强。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-12 DOI: 10.1007/s00705-025-06347-w

Mariem Bessaid, Ki Hong Kim

{"title":"Insertion of the P gene of spring viremia of carp virus into the snakehead rhabdovirus genome increases virulence.","authors":"Mariem Bessaid, Ki Hong Kim","doi":"10.1007/s00705-025-06347-w","DOIUrl":"https://doi.org/10.1007/s00705-025-06347-w","url":null,"abstract":"Spring viremia of carp virus (SVCV) is highly lethal in common carp (Cyprinus carpio) and other cyprinid fish species. In this study, we evaluated the effect of the SVCV phosphoprotein (P protein) on the virulence of snakehead rhabdovirus (SHRV) by generating a recombinant SHRV expressing the SVCV P protein. The SVCV P gene was inserted between the N and P genes of the SHRV genome, resulting in the generation of rSHRV-A-Psvcv, which contains not only its own P gene but also the P gene of SVCV. In preliminary experiments, we determined the minimum titer of SHRV that was lethal in zebrafish (Danio rerio). We then conducted experiments using titers below this threshold to assess the in vivo virulence of rSHRV-A-Psvcv in zebrafish. Under these conditions, no mortality was observed in fish infected with rSHRV-A-eGFP, a modified SHRV containing the enhanced green fluorescent protein (eGFP) gene between the N and P genes, which was used as a control. However, fish infected with rSHRV-A-Psvcv exhibited mortality accompanied by hemorrhage and ophthalmia. In a minigenome assay, the SVCV P protein failed to induce reporter protein expression when co-expressed with the SHRV N and L proteins, suggesting that the SVCV P protein is unable to form a ribonucleoprotein complex with SHRV N and L proteins. Therefore, the enhanced virulence of rSHRV-A-Psvcv resulting from the addition of the SVCV P gene can probably be attributed to the intrinsic properties of the SVCV P protein itself. Epithelioma papulosum cyprini (EPC) cells infected with rSHRV-A-Psvcv exhibited significantly lower type I interferon (IFN) responses compared to those infected with rSHRV-A-eGFP, suggesting that the SVCV P protein expressed by the recombinant SHRV inhibits type I IFN responses. Thus, the immune-suppressive activity of the SVCV P protein might be one of the causes of the enhanced virulence of rSHRV-A-Psvcv.","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"154"},"PeriodicalIF":2.5,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and evaluation of a recombinant duck enteritis virus expressing the envelope protein of duck Tembusu virus in ducks. 表达鸭天布苏病毒包膜蛋白的重组鸭肠炎病毒的构建与评价。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-10 DOI: 10.1007/s00705-025-06342-1

Lihua Wang, Xiaoyue Yang, Bing Zhang, Siyuan Hao, Yafen Song, Dan Li, Min Zhang, Qianyi Zhang, Yongda Zhao, Chenghuai Yang

引用次数: 0