Archives of Virology最新文献

{"title":"Seroepidemiology (2018–2024) and epidemic spread of an emerging human parvovirus B19 genotype 1 (subtype 1a2) variant in Hungary, 2023/2024","authors":"Fruzsina Tóth,&nbsp;Tímea Moser,&nbsp;Ákos Boros,&nbsp;Benigna Balázs,&nbsp;Károly Takáts,&nbsp;Péter Pankovics,&nbsp;Gábor Reuter","doi":"10.1007/s00705-025-06228-2","DOIUrl":"10.1007/s00705-025-06228-2","url":null,"abstract":"<div><p>An unusually large number of human parvovirus B19 (B19V) infections were reported in European countries in 2023/2024, but the genetic background of this B19V epidemic strain is unknown. In this study, there was a larger number of confirmed B19V infections (five in 2021, eight in 2023, and 59 in 2024) and higher IgG seroprevalence (41.4% in 2022 and 54.3% in 2024) in Transdanubia, Hungary, in 2024 compared to 2018-2023. A B19V genotype 1a2 variant (prototype, 1338/HUN/2024, PQ155933) with common and unique nucleotide insertions in the untranslated regions of the genome and nonsynonymous and synonymous mutations in the coding region (NS1 and VP1) could be responsible to the ongoing B19V epidemic in Europe.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-025-06228-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of a natural reassortant between Shamonda and Sathuperi viruses of the species Orthobunyavirus schmallenbergense in Japan","authors":"Hisayuki Tomochi,&nbsp;Yuji Mekaru,&nbsp;Katsunori Murota,&nbsp;Misako Konishi,&nbsp;Rina Ikeda,&nbsp;Tohru Yanase","doi":"10.1007/s00705-025-06233-5","DOIUrl":"10.1007/s00705-025-06233-5","url":null,"abstract":"<div><p>The whole-genome sequences of orthobunyaviruses isolated from cattle reared on Yonaguni Island (the western-most point of Japan's territory) were determined. The sequences of their S and L RNA segments were observed to be almost identical to those of Shamonda virus (SHAV) isolates identified in Japan, whereas the sequences of their M RNA segments were very similar to those of Japanese isolates of Sathuperi virus (SATV). Our findings indicate that the two novel isolates are natural reassortants between SHAV and SATV, which share a genome segment organization similar to that of Schmallenberg virus. The nucleotide sequence of the 5' non-coding region of the novel isolates differs from those of previously sequenced SATV isolates, suggesting that the M RNA segments of the reassortants were not derived from SATV strains that were detected recently in Japan.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic infection of cowpea aphid-borne mosaic virus in Passiflora spp. occurs at the initial stage regardless of the species’ resistance","authors":"Zanon Santana Gonçalves,&nbsp;Onildo Nunes de Jesus,&nbsp;Carlos Bernard Moreno Cerqueira-Silva,&nbsp;Ronan Xavier Correa","doi":"10.1007/s00705-025-06230-8","DOIUrl":"10.1007/s00705-025-06230-8","url":null,"abstract":"<div><p>Passion fruit woodiness disease (PWD), caused by cowpea aphid-borne mosaic virus (CABMV), severely damages leaves and fruits, compromising passion fruit production. The dynamics of this infection in <i>Passiflora</i> spp. are still poorly understood. The objective of this study was to determine the systemic infection time of CABMV in <i>Passiflora</i> spp. and to quantify the viral titer throughout the infection. Plants of <i>Passiflora edulis</i> Sims. (BGP418, susceptible), <i>P. cincinnata</i> Mast. (BGP243, moderately resistant), <i>P. setacea</i> DC. (BRS Pérola do Cerrado, resistant), and <i>P. suberosa</i> L. (BGP152, resistant) were used. The study was conducted in a climate chamber, and mechanical inoculations were carried out on the first pair of basal leaves of the seedlings. Symptoms were assessed using a scale whose scores were converted into a disease index (DI%), and the viral titer was determined at different time points by real-time quantitative RT-PCR (RT-qPCR). The first symptoms of the virus were observed at seven days after inoculation (Dai) in <i>P. edulis</i> (DI = 5.15%) and at 10 Dai in <i>P. cincinnata</i> (DI = 8.86%). On the other hand, <i>P. setacea</i> and <i>P. suberosa</i> did not show typical symptoms of the disease (DI = 0.00%). Systemic CABMV infection was detected at 30 minutes after inoculation regardless of the level of resistance of the <i>Passiflora</i> species. There was an increase in viral titer with infection time with <i>P. edulis</i> and <i>P. cincinnata</i>, although in the case of <i>P. edulis</i>, the increase in CABMV titer occurred earlier, at 2 Dai, and in <i>P. cincinnata</i> at 8 Dai. In the asymptomatic species (<i>P. setacea</i> and <i>P. suberosa</i>), there was no variation in the viral titer over the time periods evaluated. This pioneering study provides information for the selection of time intervals for future molecular research into the interaction between <i>Passiflora</i> spp. and CABMV.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology of chicken and turkey parvovirus in Turkiye: high risk, genetic recombination signals, and first complete genome analysis","authors":"Hasan Abayli,&nbsp;Ahsen Nisa Aslan,&nbsp;Sukru Tonbak,&nbsp;Hasan Ongor,&nbsp;Mehmet Akan","doi":"10.1007/s00705-025-06229-1","DOIUrl":"10.1007/s00705-025-06229-1","url":null,"abstract":"<div><p>The aim of this study was to detect chicken parvovirus (ChPV) and turkey parvovirus (TuPV) on Turkish poultry farms and examine the molecular epidemiology of these viruses. In 2023, a total of 1,060 fecal samples were collected from 76 broiler farms and 30 turkey farms across various regions of Turkiye. The overall positivity rate was 72.3% (55/76) in broiler flocks and 70% (21/30) in turkey flocks. Among these, the positivity rate was 35% (7/20) in birds showing no signs of enteritis. The findings of this study suggest that, depending on the genetic variability of regional strains or the viral load, nested-PCR-based methods may prove more effective than single-step PCR for detecting ChPV and TuPV cases. Therefore, it is recommended to incorporate nested PCR in molecular epidemiological studies to minimize the risk of overlooking positive cases. Phylogenetic analysis of the VP1, VP2, and complete genome sequences of TUR/2023/ChPV and TR/2023/TuPV revealed that both strains clustered with American strains in distinct lineages. Analysis using the recombination detection programs RDP4.0 and SimPlot 3.5.1 strongly suggested that TUR/2023/ChPV may be a recombinant of strains from China and Brazil. Despite the observed genetic variability in the VP2 protein, an examination of B-cell epitopes showed a high degree of similarity between ChPV and TuPV strains. This study represents the first documentation of the emergence and prevalence of TuPVs in Turkiye, providing complete genome sequences for both ChPV and TuPV. Given the high prevalence and genetic diversity of these viruses and their presence in clinically asymptomatic birds, it is crucial for stakeholders in the poultry industry to implement regular flock screenings for these and similar viral pathogens. Proactive measures like these are essential for mitigating the economic impact of these important pathogens and developing effective preventive strategies.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral metagenomics in wild ducks reveals the presence of seadornaviruses in Siberia","authors":"Nikita Dubovitskiy,&nbsp;Anastasiya Derko,&nbsp;Arina Loginova,&nbsp;Anna Khozyainova,&nbsp;Evgeny Denisov,&nbsp;Maxim Apanasevich,&nbsp;Alina Kokhanenko,&nbsp;Alexey Druzyaka,&nbsp;Alexander Shestopalov,&nbsp;Kirill Sharshov","doi":"10.1007/s00705-025-06226-4","DOIUrl":"10.1007/s00705-025-06226-4","url":null,"abstract":"<div><p><i>Seadornavirus</i> is a genus of mosquito-borne viruses that includes Banna virus, which was first discovered in human patients with encephalitis and fever, as well as Kadipiro virus and Liao ning virus. In this study, we used metagenomics to investigate the diversity of viruses in wild ducks and detected both Banna virus and Kadipiro virus in wild birds in Siberia. These data suggest that seadornaviruses, which were previously only found in South East Asia, are also circulating in Northern Eurasia.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic analysis and molecular structure of NS1 proteins of porcine parvovirus 5 isolates from Mexico","authors":"Alejandro Vargas-Ruiz,&nbsp;Diana Michele Araiza-Hernández,&nbsp;Francisco Rodolfo González-Díaz,&nbsp;Ernesto Marín-Flamand,&nbsp;José Ivan Sánchez Betancourt,&nbsp;Ana Elvia Sánchez-Mendoza,&nbsp;Lucia Angélica García-Camacho","doi":"10.1007/s00705-024-06182-5","DOIUrl":"10.1007/s00705-024-06182-5","url":null,"abstract":"<div><p>Porcine parvovirus 5 (PPV5) is an unclassified member of the family <i>Parvoviridae</i> with no reported pathogenicity, although it is associated with multisystemic, reproductive, and respiratory diseases. Its open reading frame 1 (ORF1) encodes non-structural protein 1 (NS1), which is predicted to have helicase activity that is essential for viral replication. This protein contains a C-motif with an invariant asparagine residue that forms the core of the enzyme's active site, in conjunction with the Walker A and B motifs. The aim of this study was the phylogenetic and molecular characterization of the NS1 of PPV5 through nested PCR and sequencing of three Mexican PPV5-positive samples. Subsequently, a phylogenetic tree, identity matrices of nucleotide and amino acid sequences, and a three-dimensional model of NS1 were constructed. The amplified sequences, which represented 96.9% of the PPV5 ORF1, occupied the same branch in the phylogenetic tree and exhibited the most nucleotide sequence similarity to the corresponding region of PPV4 and the most amino acid sequence similarity to the NS1 proteins of PPV4 and PPV6. A three-dimensional model of NS1 displayed a C-motif characteristic of superfamily 3 (SF3) helicases. The phylogenetic proximity of PPV5 to PPV4 and PPV6 suggests that it may belong to the genus <i>Copiparvovirus</i>. Further studies on helicases from viruses infecting domestic animals may be useful in developing antiviral drugs for both human and veterinary medicine.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequence analysis and genome organization of a new marafivirus from Leptochloa chinensis","authors":"Muqmirah,&nbsp;Guangming Xiao,&nbsp;Pengpeng Jie,&nbsp;Zhongtian Xu,&nbsp;Taiyun Wei,&nbsp;Hui Wang","doi":"10.1007/s00705-025-06220-w","DOIUrl":"10.1007/s00705-025-06220-w","url":null,"abstract":"<div><p>High-throughput sequencing was used to identify and characterize a novel marafivirus from the weed <i>Leptochloa chinensis</i>, which was tentatively named \"Leptochloa chinensis marafivirus\" (LcMV). The complete genome of the virus consists of 6,178 base pairs, and its nucleotide sequence is 73.82% identical to that of Sorghum almum marafivirus, which is a member of the genus <i>Marafivirus</i> within the family <i>Tymoviridae</i>. The LcMV genome contains a relatively large open reading frame (ORF) encoding a single polyprotein (220.6 kDa) with five functional domains (methyltransferase, papain-like protease, helicase, RNA-dependent RNA polymerase, and coat proteins), which is a characteristic of members of this genus. Furthermore, a 16-nucleotide conserved marafibox sequence was identified at nucleotide positions 5341–5356. The coat protein of LcMV is 68.02% identical to that of Sorghum almum marafivirus. Phylogenetic analysis based on nucleotide and polyprotein sequences showed that LcMV is closely related to members of the genus <i>Marafivirus</i>. Our findings support the classification of LcMV as a member of a new species within this genus. This is the first report of a marafivirus infecting <i>Leptochloa chinensis</i>, a very important weed of rice.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First complete genome sequence of tulip mild mottle mosaic virus (Ophiovirus tulipae)","authors":"Yutaro Neriya,&nbsp;Kakeru Hamamoto,&nbsp;Tominari Kobayashi,&nbsp;Shunsuke Nakase,&nbsp;Rena Kurosawa,&nbsp;Tomohiro Suzuki,&nbsp;Hisashi Nishigawa,&nbsp;Toshiyuki Morikawa,&nbsp;Tomohide Natsuaki","doi":"10.1007/s00705-025-06224-6","DOIUrl":"10.1007/s00705-025-06224-6","url":null,"abstract":"<div><p>Tulip mild mottle mosaic disease, caused by tulip mild mottle mosaic virus (TMMMV, species <i>Ophiovirus tulipae</i>), was first reported in Japan in 1979. TMMMV has a negative-sense ssRNA genome and is closely related to ophioviruses such as Mirafiori lettuce big vein virus (MLBVV, <i>Ophiovirus mirafioriense</i>). However, its complete nucleotide sequence has not yet been reported. Therefore, in this study, we determined the complete nucleotide sequences of all four TMMMV segments. The 5′-terminal tetranucleotide sequences were highly conserved, and the five bases at the 5′ end were identical in all segments. Nucleotide sequence comparisons of the four segments showed the highest sequence identity to those of MLBVV (70–76% for the genomic RNA sequences and 81–87% for the amino acid sequences of each ORF, except for ORF2). The amino acid sequences of the coat proteins of TMMMV and MLBVV were found to be 83.1% identical, which is below the 85% threshold for assignment to the same species, indicating that these viruses belong to different species in the same genus.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-025-06224-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Genome sequencing and characterization of three Bacillus cereus-specific phages, DK1, DK2, and DK3","authors":"Li Kong,&nbsp;Yu Ding,&nbsp;Qingping Wu,&nbsp;Juan Wang,&nbsp;Jumei Zhang,&nbsp;Hongye Li,&nbsp;Shubo Yu,&nbsp;Pengfei Yu,&nbsp;Tiantian Gao,&nbsp;Haiyan Zeng,&nbsp;Meiyan Yang,&nbsp;Yongjian Liang,&nbsp;Zhi Wang,&nbsp;Zhiqing Xie,&nbsp;Qianwen Wang","doi":"10.1007/s00705-024-06216-y","DOIUrl":"10.1007/s00705-024-06216-y","url":null,"abstract":"","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human bocavirus infections in paediatric patients in a tertiary care hospital in Kerala, India","authors":"B. Aneesh,&nbsp;Swapna K. Pillai,&nbsp;P. S. Chippy,&nbsp;Megha Chandran,&nbsp;Arun V. Jose,&nbsp;Lalitha Kailas,&nbsp;M. Neziya,&nbsp;S. Aswathyraj,&nbsp;E. Sreekumar","doi":"10.1007/s00705-024-06218-w","DOIUrl":"10.1007/s00705-024-06218-w","url":null,"abstract":"<div><p>Human bocaviruses (HBoVs) can cause respiratory illness in young children. Although the first HBoV infection in India was reported in 2010, very little information is available about its prevalence, clinical features, or geographic distribution in this country. This study was conducted using 136 respiratory samples from paediatric patients in a tertiary care hospital in Kerala, 21 of which tested positive for HBoV1 and were further characterized through VP1/VP2 gene sequencing. We found that different strains of HBoV1 are co-circulating in the region and that HBoV1 can be detected in children with severe acute respiratory infections, either alone or coinfections with other pathogens, without any significant differences in their clinical characteristics.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}