Archives of Virology最新文献_第2页

Complete genome sequence of a novel narnavirus with inverted complementary termini from the rice blast fungus Magnaporthe oryzae isolate NJ471. 稻瘟病菌Magnaporthe oryzae分离株NJ471具有反向互补末端的新型narnavvirus全基因组序列。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-08 DOI: 10.1007/s00705-025-06337-y

Cong Li, Yuxin Wu, Xinyi Li, Qingchao Deng, Jiatao Xie

引用次数: 0

Single-particle quantification of SARS-CoV-2 virus-like particles using flow virometry. 用流动病毒学对SARS-CoV-2病毒样颗粒进行单颗粒定量。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-07 DOI: 10.1007/s00705-025-06339-w

Hyeop Jin, Ye Rae Cho, Yong Tae Jung

{"title":"Single-particle quantification of SARS-CoV-2 virus-like particles using flow virometry.","authors":"Hyeop Jin, Ye Rae Cho, Yong Tae Jung","doi":"10.1007/s00705-025-06339-w","DOIUrl":"https://doi.org/10.1007/s00705-025-06339-w","url":null,"abstract":"SARS-CoV-2 virus-like particles (VLPs) were generated by co-transfecting HEK 293T cells with four plasmids, two for expression of the spike (S) or nucleocapsid (N) protein with a 3xHA tag at the C-terminus, one for expression of the membrane (M) protein, and one for expression of the envelope (E) protein. Co-localization of the S and N proteins within the VLPs was confirmed by Western blot analysis using anti-HA antibodies. To enable fluorescent quantification of VLPs, the C-terminus of the S protein was fused to enhanced green fluorescent protein (EGFP), and the N protein was tagged with EGFP at either the N- or C-terminus. Transient transfection of 293T cells with (S-GFP)NME, S(N-GFP)ME, or S(GFP-N)ME plasmids efficiently produced fluorescent VLPs, each demonstrating the ability to enter A549-hACE2 cells. In addition, a two-plasmid system was developed to simplify fluorescent VLP production by incorporating internal ribosomal entry site elements between N3xHA and S-GFP and between the M and E genes. Transfection of 293T cells with these plasmids produced VLPs with a fourfold higher N protein concentration (800 ng/mL) compared to the four-plasmid system (200 ng/mL), as measured by ELISA. Flow virometry analysis confirmed production of VLPs with an average diameter of 80 nm and a concentration of 1.68 × 108 particles/ml. This study demonstrates that production of fluorescent VLPs using a two-plasmid system is more efficient than the traditional four-plasmid approach. We also demonstrate that flow virometry is a robust method for single-particle analysis of fluorescent VLPs for determining their size and concentration.","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"149"},"PeriodicalIF":2.5,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of chenopodium trirhavirus 1, a novel tri-segmented virus of the family Rhabdoviridae. 横纹肌病毒科一种新型三节段病毒chenopodium trirhavirus 1的鉴定。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-07 DOI: 10.1007/s00705-025-06344-z

Xinyu Qin, Zhihong Guo, Zhangyao Nie, Yizhou Yang, Ruiqi Zhang, Xianbing Wang, Zongying Zhang, Chenggui Han, Ying Wang

{"title":"Characterization of chenopodium trirhavirus 1, a novel tri-segmented virus of the family Rhabdoviridae.","authors":"Xinyu Qin, Zhihong Guo, Zhangyao Nie, Yizhou Yang, Ruiqi Zhang, Xianbing Wang, Zongying Zhang, Chenggui Han, Ying Wang","doi":"10.1007/s00705-025-06344-z","DOIUrl":"https://doi.org/10.1007/s00705-025-06344-z","url":null,"abstract":"A novel tri-segmented virus, tentatively named \"Chenopodium trirhavirus 1\" (CheTRV1), was identified in Chenopodium album in China using high-throughput sequencing combined with conventional Sanger sequencing after RT-PCR. The genome of CheTRV1 consists of three negative-sense single-stranded RNAs designated as RNA1, RNA2, and RNA3. RNAs 1-3 of CheTRV1 share 57.93-64.41%, 48.16-56.96%, and 38.12-42.22% nucleotide sequence identity with the corresponding segments of previously reported trirhaviruses. The 3'- and 5'-termini of the three segments are similar and exhibit partial inverse complementarity. The ORFs of CheTRV1 are separated by a conserved gene junction region with the consensus motif 3'-AAUUCUUUUGN(N)nUUC- 5'. RNA1 consists of 6749 nucleotides (nt) and encodes a single putative L protein. RNA2 is 4393 nt in length and contains four genes in the order 3'-N-P2-P3-P4-5'. The smaller RNA3 consists of 3897 nt and has three genes in the order 3'-P6-P7-P8-5'. Phylogenetic analysis and pairwise comparisons suggested that CheTRV1 is most closely related to Medicago trirhavirus 1, with 68.82% amino acid sequence identity in the L protein. This is the first report of the complete genome sequence of a tri-segmented rhabdovirus, and it expands our knowledge of rhabdovirus evolution.","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"151"},"PeriodicalIF":2.5,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurovirulence of Zika virus-encoded proteins. 寨卡病毒编码蛋白的神经毒力。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-07 DOI: 10.1007/s00705-025-06338-x

Zi-Hui Ma, Li Xing

引用次数: 0

Molecular characterization of a novel non-segmented double stranded RNA mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea. 一种感染植物病原真菌葡萄球孢的新型非节段双链RNA分枝病毒的分子特征。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-06 DOI: 10.1007/s00705-025-06324-3

Shunpei Xie, Yanan Zhang, Guoqing Xing, Qinzhou Ma, Yashuang Guo, Rui Zang, Chao Xu, Haiyan Wu, Haiqiang Li, Meng Zhang

{"title":"Molecular characterization of a novel non-segmented double stranded RNA mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea.","authors":"Shunpei Xie, Yanan Zhang, Guoqing Xing, Qinzhou Ma, Yashuang Guo, Rui Zang, Chao Xu, Haiyan Wu, Haiqiang Li, Meng Zhang","doi":"10.1007/s00705-025-06324-3","DOIUrl":"https://doi.org/10.1007/s00705-025-06324-3","url":null,"abstract":"Botryosphaeria dothidea is a prevalent pathogen of woody plants with a global distribution. In this study, we identified a novel mycovirus from the B. dothidea strain ZM200473, which we have tentatively designated \"Botryosphaeria dothidea non-segmented dsRNA virus\" (BdNSRV1). The genome of BdNSRV1 is composed of dsRNA that spans 2,902 base pairs and contains two non-overlapping open reading frames (ORF1 and ORF2). ORF1 encodes a hypothetical protein consisting of 316 amino acids with a molecular weight of 35.0 kDa, and this protein shares similarity to the coat proteins of several mycoviruses. ORF2 encodes a protein of 561 amino acids with a molecular weight of 66.0 kDa, which includes a conserved RNA-dependent RNA polymerase (RdRp). Sequence comparisons and phylogenetic analysis indicated that BdNSRV1 establishes a well-supported independent clade alongside members of the recently established genus Unirnavirus, being most closely related to Lasiodiplodia pseudotheobromae mycovirus 1 (LpMyV1), with 70.74% amino acid sequence identity in the RdRp. Therefore, BdNSRV1 should be classified as a novel non-segmented dsRNA mycovirus of the genus Unirnavirus and is the first characterized non-segmented virus associated with B. dothidea.","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"148"},"PeriodicalIF":2.5,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the mycovirus Aspergillus sulphureus partitivirus 1. 分支病毒硫曲霉局部病毒1的鉴定。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-03 DOI: 10.1007/s00705-025-06333-2

Seiji Buma, Syun-Ichi Urayama, Kenji Tomita, Sayoko Oiki, Shigeru Okada, Akihiro Ninomiya

{"title":"Characterization of the mycovirus Aspergillus sulphureus partitivirus 1.","authors":"Seiji Buma, Syun-Ichi Urayama, Kenji Tomita, Sayoko Oiki, Shigeru Okada, Akihiro Ninomiya","doi":"10.1007/s00705-025-06333-2","DOIUrl":"10.1007/s00705-025-06333-2","url":null,"abstract":"Fungi are frequently infected with viruses called mycoviruses. Some mycoviruses have shown potential as biocontrol agents because they can weaken the virulence of fungal plant pathogens. Intensive research has been conducted on the diversity of viruses infecting plant pathogens and the functions of such viruses. In contrast, the diversity of viruses infecting fungi that are not plant pathogens is less well understood. We previously identified a mycovirus of the family Partitiviridae, Aspergillus sulphureus partitivirus 1 (AsuPV1), from an Aspergillus sulphureus strain isolated from fermented dried bonito. In the present study, we conducted a detailed phylogenetic analysis of AsuPV1 and determined its molecular characteristics. The phylogenetic analysis revealed that AsuPV1 belongs to the proposed subgroup I within the genus Gammapartitivirus. Virus particles of AsuPV1 were isolated by density gradient centrifugation, and molecular analyses indicated that each of its three genome segments is encapsulated in capsids composed of the putative viral coat protein. AsuPV1 is the second virus with a tri-segmented genome to be found in a member of Gammapartitivirus subgroup I, after Penicillium stoloniferum virus F. A phenotypic comparison between the parental A. sulphureus strain and isogenic virus-free isolates indicated that AsuPV1 enhances conidium production in its host. This study provides insight into the diversity and molecular characteristics of partitiviruses with a tri-segmented genome.","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"147"},"PeriodicalIF":2.5,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The complete nucleotide sequence and genome organization of a novel wheat-infecting luteovirus. 一种新型小麦感染鲁特病毒的全核苷酸序列和基因组组织。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-02 DOI: 10.1007/s00705-025-06336-z

Zhiguo Zhou, Xue Feng, Linzhu Liang, Wenya Liu, Zixuan Zhou, Xinyuan Zhang, Zhongtian Xu, Peipei Zhang

引用次数: 0

Isolation and molecular identification of canine bufavirus: a novel enteric pathogen of dogs. 犬兔病毒：一种新型犬肠道病原体的分离与分子鉴定。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-06-02 DOI: 10.1007/s00705-025-06328-z

Gulizar Acar, Hayrunnisa Bostan Yoru, Erdal Polat, Ahsen Nisa Aslan, Hakan Aydin, Mehmet Ozkan Timurkan

{"title":"Isolation and molecular identification of canine bufavirus: a novel enteric pathogen of dogs.","authors":"Gulizar Acar, Hayrunnisa Bostan Yoru, Erdal Polat, Ahsen Nisa Aslan, Hakan Aydin, Mehmet Ozkan Timurkan","doi":"10.1007/s00705-025-06328-z","DOIUrl":"https://doi.org/10.1007/s00705-025-06328-z","url":null,"abstract":"Canine bufavirus (CBuV) was initially detected in dogs with respiratory symptoms and later identified in fecal samples, suggesting that it could be an enteric pathogen. Several studies have shown that CBuV can be present in both healthy and diarrheic dogs, raising questions about whether CBuV is a primary enteric pathogen. In this study, we identified, isolated, and characterized CBuV from swab samples from dogs. A total of 119 samples from diarrheic dogs were analyzed by PCR, targeting a portion of the VP2 gene of CBuV. CBuV was detected in five dogs (4.2%). For virus isolation, CBuV-positive samples were inoculated onto an MDCK cell culture, and CPE was observed for one sample, which was tested for CBuV as well as other frequently detected enteric pathogens (canine distemper virus, canine parvovirus type 2, and canine coronavirus), but CBuV was the only virus detected. Phylogenetic analysis indicated that the CBuV isolates from this study are closely related to strains from China and India. The detection of CBuV in this study suggests that it might play a distinct role in canine diarrhea. Further studies are needed to investigate its genetic characteristics, epidemiology, and biological significance.","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"146"},"PeriodicalIF":2.5,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic analysis of the novel Stenotrophomonas phage vB_Srh_LBjhp91a, isolated from a Karst cave in China. 中国喀斯特溶洞新型窄养单胞菌噬菌体vB_Srh_LBjhp91a的基因组分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-29 DOI: 10.1007/s00705-025-06329-y

Lou Ren, Zheng Fang, Shixia Li, Qingshan Wu, Lan Xiang, Chuangen Lin, Qiuping Liu, Leitao Tan, Qingbei Weng

引用次数: 0

Novel torque teno viruses infecting raccoon dogs (Nyctereutes procyonoides) and red foxes (Vulpes vulpes japonica). 感染貉（原yonoides）和红狐（Vulpes Vulpes japonica）的新型转矩病毒。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-29 DOI: 10.1007/s00705-025-06316-3

Shoko Nishiyama, Yuji Fujii, Keisuke Nakagawa, Tomoya Shichijo, Makoto Asano, Shigeru Tajima, Chang Kweng Lim, Tatsunori Masatani, Naoto Ito

{"title":"Novel torque teno viruses infecting raccoon dogs (Nyctereutes procyonoides) and red foxes (Vulpes vulpes japonica).","authors":"Shoko Nishiyama, Yuji Fujii, Keisuke Nakagawa, Tomoya Shichijo, Makoto Asano, Shigeru Tajima, Chang Kweng Lim, Tatsunori Masatani, Naoto Ito","doi":"10.1007/s00705-025-06316-3","DOIUrl":"https://doi.org/10.1007/s00705-025-06316-3","url":null,"abstract":"Complete genome sequences of four torque teno virus (TTV) (family Anelloviridae) isolates were obtained from the feces of two raccoon dogs (Nyctereutes procyonoides) and two red foxes (Vulpes vulpes) in Gifu Prefecture, Japan. The ORF1 nucleotide sequences of these four viruses, named Raccoon dog_Fe_1, Raccoon dog_Fe_2, Fox_Fe_1, and Fox_Fe_2, were different from those of known TTVs but similar to those of TTVs derived from masked palm civet_Pl-TTV9-1 (59.8 %), masked palm civet_Pl-TTV3 (56.7%), masked palm civet_Pl-TTV9-2 (70.6 %), and crab-eating fox_LV23 strain (64.7 %), respectively, indicating that Raccoon dog_Fe_1, Raccoon dog_Fe_2, and Fox_Fe_2 represent new species. Phylogenetic analysis based on amino acid sequences of the ORF1 protein revealed that Fox_Fe_1 and Fox_Fe_2 clustered together with crab-eating fox_LV23 from Brazil and were distinct from viruses from domestic dogs. Furthermore, Raccoon dog_Fe_2 did not belong to any canine animal TTVs cluster. In contrast, Raccoon dog_Fe_1 clustered together with pampas fox_LV13, and these viruses were distant from other canid animal TTVs. Therefore, wild-canid TTVs formed several distinct clusters even at different geological locations such as Brazil and Japan.","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"144"},"PeriodicalIF":2.5,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0