Archives of Virology最新文献_第2页

Molecular characterization of porcine reproductive and respiratory syndrome virus in Henan and Shanxi, China, during 2023–2024 2023-2024年中国河南和山西猪繁殖与呼吸综合征病毒的分子特征

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-12 DOI: 10.1007/s00705-025-06380-9

Tao Wang, Xuan-Ang Wang, Jia-Qi Zhang, Lan-Lan Zheng, Ping-Li Wang, Hong-Ying Chen, Shi-Jie Ma

{"title":"Molecular characterization of porcine reproductive and respiratory syndrome virus in Henan and Shanxi, China, during 2023–2024","authors":"Tao Wang, Xuan-Ang Wang, Jia-Qi Zhang, Lan-Lan Zheng, Ping-Li Wang, Hong-Ying Chen, Shi-Jie Ma","doi":"10.1007/s00705-025-06380-9","DOIUrl":"10.1007/s00705-025-06380-9","url":null,"abstract":"<div><p>Porcine reproductive and respiratory syndrome virus (PRRSV) is responsible for one of the most prevalent and economically damaging diseases in the pig industry. Surveillance of its epidemic profile and genetic Characteristics is critical for developing effective control strategies. In this study, 213 clinical samples were collected from pigs displaying typical symptoms of porcine reproductive and respiratory syndrome from swine farms of varying sizes in 13 cities of Henan and Shanxi provinces, China, between 2023 and 2024. All samples were screened via RT-PCR, and the ORF5 and Nsp2 genes of the viruses found in positive samples were sequenced and analyzed. The results revealed a PRRSV positivity rate of 19.72% (42/213). Phylogenetic analysis based on ORF5 showed that all 35 sequenced strains belong to the genotype PRRSV-2, with 27 strains clustering within sublineage 1.8 (NADC30-like), five in sublineage 5.1 (VR2332-like), and the remaining strains distributed among sublineages 8.7 (HP-PRRSV-like), 1.5 (NADC34-like), and 3.5 (QYYZ-like), indicating the dominance of NADC30-like strains in Henan and Shanxi provinces. Additionally, Nsp2 gene analysis of 10 selected strains (sublineages 5.1 and 1.8) revealed a unique 131-amino-acid (aa) deletion in the hypervariable region. Notably, two novel discontinuous deletions were identified: a 7-aa deletion (PVSLGGD) at positions 216-222 and a 3-aa deletion (GVL) at positions 453-455. Additionally, strains HNLY-6, HNLY-11, and HNLY-14 exhibited evidence of possible recombination events between NADC30-like and VR2332-like strains. These findings provide critical insights into the epidemiology of PRRSV in Henan and Shanxi provinces from 2023 to 2024, supporting the development of targeted prevention and control strategies.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting pathogenicity of an epizootic hemorrhagic disease virus serotype 7 isolate: high virulence in IFNAR(-/-) mice versus a negligible role of sheep in EHDV-7 epidemiology 动物流行性出血病病毒血清型7分离物的致病性对比：IFNAR（-/-）小鼠的高毒力与绵羊在EHDV-7流行病学中的可忽略作用

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-11 DOI: 10.1007/s00705-025-06377-4

Junyong Guan, Yinglin Qi, Yingran Huang, Ran Shao, Dong Zhou, Cankun Xi, Xin Yin

引用次数: 0

Small RNAs derived from avocado sunblotch viroid and their association with bleaching symptoms: implications for pathogenesis in avocado sunblotch disease 牛油果斑病类病毒衍生的小rna及其与白化症状的关联：牛油果斑病发病机制的意义

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-09-11 DOI: 10.1007/s00705-025-06360-z

Melissa Joubert, Noëlani van den Berg, Jacques Theron, Velushka Swart

{"title":"Small RNAs derived from avocado sunblotch viroid and their association with bleaching symptoms: implications for pathogenesis in avocado sunblotch disease","authors":"Melissa Joubert, Noëlani van den Berg, Jacques Theron, Velushka Swart","doi":"10.1007/s00705-025-06360-z","DOIUrl":"10.1007/s00705-025-06360-z","url":null,"abstract":"<div>\u0000 \u0000 <p>Avocado sunblotch viroid (ASBVd) is a structured RNA molecule responsible for sunblotch disease of avocado, characterised by distinct chloroses of fruit, leaves, and stems. Despite its impact on avocado, the mechanism by which ASBVd elicits sunblotch symptoms remains unknown. Previous studies on other avsunviroids have shown that viroid-derived small RNAs (vd-sRNAs) with specific sequence mutations can trigger leaf chlorosis via RNA silencing of host genes. Building on this knowledge, we aimed to shed light on the molecular basis of ASBVd pathogenesis by analysing ASBVd sequence variants and ASBVd-sRNAs from bleached and asymptomatic leaf tissues of sunblotch-affected avocado trees. Sequencing of ASBVd clones revealed that variants carrying the pathogenic determinant for bleaching were present in both green and yellow leaf tissues. Next-generation sequencing (NGS) identified ASBVd-sRNAs that varied in abundance between symptomatic and asymptomatic leaf tissues, correlating with viroid titre. We discovered 64 vd-sRNAs spanning the pathogenic region of the ASBVd genome, which were almost exclusively found in yellow tissues. The ASBVd-sRNAs containing the bleaching-associated mutation were predicted to target numerous avocado transcripts for degradation, with 25 of these transcripts significantly downregulated in bleached tissues. Notably, one of these genes, encoding a chloroplastic protein, demonstrated strong evidence of ASBVd-sRNA-guided RNA silencing, presenting a promising candidate for future research into the molecular trigger for ASBVd-induced bleaching symptoms. This study is the first to investigate ASBVd-sRNAs in bleached leaves using NGS. Our findings support the role of RNA silencing in sunblotch symptom development and reveal a unique silencing trigger compared to other avsunviroids.</p>\u0000 </div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-025-06360-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the genomes of Vibrio phages pVco-5 and pVco-7: implications for phage-host interactions 噬菌体弧菌pVco-5和pVco-7基因组的比较：对噬菌体-宿主相互作用的影响

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-08-27 DOI: 10.1007/s00705-025-06386-3

Hyoun Joong Kim, Jun Kwon, Chan Hee Lee, Ji Hyung Kim, Se Chang Park, Sang Guen Kim

引用次数: 0

Genetics of natural recombinant infectious bronchitis viruses isolated in Kagoshima Prefecture of Japan 日本鹿儿岛县分离的天然重组传染性支气管炎病毒的遗传学

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-08-27 DOI: 10.1007/s00705-025-06389-0

Taichiro Tanikawa, Masaji Mase, Kanae Hiramatsu, Satoko Watanabe, Hiroshi Iseki

{"title":"Genetics of natural recombinant infectious bronchitis viruses isolated in Kagoshima Prefecture of Japan","authors":"Taichiro Tanikawa, Masaji Mase, Kanae Hiramatsu, Satoko Watanabe, Hiroshi Iseki","doi":"10.1007/s00705-025-06389-0","DOIUrl":"10.1007/s00705-025-06389-0","url":null,"abstract":"<div><p>Avian infectious bronchitis (IB), caused by IB virus (IBV; family <i>Coronaviridae</i>, genus <i>Gammacoronavirus</i>, species <i>Gammacoronavirus galli</i>), is a highly contagious and acute upper respiratory tract disease in chickens (<i>Gallus gallus</i>) that has a serious economic impact worldwide. Genomic mutations and recombination of IBV strains result in novel pathogenicity phenotypes. This study focused on three IBV strains isolated from a farm in Kagoshima Prefecture in Japan (KS2–4) and two from the surrounding area (KS and KS1). These strains were evaluated using whole-genome sequencing, followed by detection of genomic recombination events and phylogenetic analyses. Genomic recombination analysis using nearly complete genome sequences revealed that the candidate donor strains, defined as those contributing recombinant segments to the detected isolates, for KS2, KS3, and KS4 were identical except in some regions. Furthermore, KS and KS1 were predicted to have different donor strains across their genomic regions despite being classified as belonging to the same genotype based on the <i>S1</i> gene. Phylogenetic analysis revealed that KS and KS1 are a variant of a vaccine strain and a previously unreported recombinant strain, respectively. KS2–4 were found to be multi-recombinant strains that may have experienced several different recombination events with unknown strains, including recombinant strains with complex genetic backgrounds. These results suggest that unidentified IBV strains with various genetic backgrounds may have circulated in Japan and that multiple genetic recombination events may have occurred among these strains.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two cross-neutralizing antibodies isolated from a COVID-19 convalescent via single B cell sorting 通过单B细胞分选从COVID-19恢复期患者中分离到两种交叉中和抗体

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-08-25 DOI: 10.1007/s00705-025-06372-9

Yuanyuan Hu, Caiqin Hu, Junwei Su, Biao Zhu, Hao Liang, Yiming Shao

{"title":"Two cross-neutralizing antibodies isolated from a COVID-19 convalescent via single B cell sorting","authors":"Yuanyuan Hu, Caiqin Hu, Junwei Su, Biao Zhu, Hao Liang, Yiming Shao","doi":"10.1007/s00705-025-06372-9","DOIUrl":"10.1007/s00705-025-06372-9","url":null,"abstract":"<div><p>The ongoing emergence of Omicron subvariants, which exhibit significant resistance to existing therapeutic antibodies, underscores the urgent need to develop new reagents capable of broadly and effectively neutralizing current subvariants. Antigen-specific memory B cells were sorted by flow cytometry. The heavy and light variable region genes of monoclonal antibodies (mAbs) were amplified and cloned into expression vectors. Following protein expression, the binding and neutralizing activities of the mAbs were assessed using ELISA, biolayer interferometry (BLI), and neutralization assays. Additionally, the neutralizing mechanism of mAb C12 was analyzed through protein modeling. We identified two receptor-binding domain (RBD)-targeting cross-neutralizing antibodies (cross-NAbs), B5 and C12. C12 demonstrated potent neutralization against SARS-CoV-2 wild type (WT), BA.1, BA.2, BA.4/5, and BF.7, with IC<sub>50</sub> values below 0.0300 µg/mL, and against XBB and EG.5, with IC<sub>50</sub> values of 0.2340 µg/mL and 0.2762 µg/mL, respectively. B5 exhibited strong neutralizing activity against WT, BA.1, and BA.2, with IC<sub>50</sub> values below 0.0900 µg/mL, and moderate neutralizing ability against BA.4/5, XBB, and EG.5. BLI experiments revealed that both B5 and C12 bind strongly to the RBD of WT and BA.1, with K<sub>D</sub> values below 1.0 × 10<sup>−12</sup> M. Structural modeling of the C12-RBD complex suggested that the full contact of the heavy chain complementarity-determining region 3 (HCDR3) loop with the RBD, together with region between the light chain complementarity-determining region 3 (LCDR3) and the RBD, may contribute to the cross-neutralizing activity of C12. These findings highlight the therapeutic potential of B5 and C12 against evolving Omicron subvariants and provide insights for antibody engineering and vaccine design.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of a novel narnavirus from the plant-pathogenic fungus Colletotrichum gloeosporioides 植物病原菌炭疽菌中一种新型纳纳病毒的分子特性研究

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-08-25 DOI: 10.1007/s00705-025-06391-6

Peiping Luo, Xuetuan Lin, Siyu Zhou, Daipeng Chen, Yinhui Sun, Li Zheng

引用次数: 0

Molecular identification of a new monopartite begomovirus isolated from eggplant (Solanum melongena L.) with yellow mosaic symptoms in Nigeria 尼日利亚一种具有黄色花叶病症状的茄子单株begomavirus的分子鉴定

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-08-25 DOI: 10.1007/s00705-025-06390-7

Olawale Arogundade, John O. Oladokun, P. Lava Kumar, Olufemi J. Alabi

引用次数: 0

The mechanism of action of micafungin against pteropine orthoreovirus infection in the human A549 cell line micafungin抗人A549细胞系翼鸟类正呼肠孤病毒感染的作用机制

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-08-25 DOI: 10.1007/s00705-025-06369-4

Wirayatida Bubphasook, Atsuo Iida, Eiichi Hondo

{"title":"The mechanism of action of micafungin against pteropine orthoreovirus infection in the human A549 cell line","authors":"Wirayatida Bubphasook, Atsuo Iida, Eiichi Hondo","doi":"10.1007/s00705-025-06369-4","DOIUrl":"10.1007/s00705-025-06369-4","url":null,"abstract":"<div><p>Pteropine orthoreovirus (PRV) is a fusogenic virus carried by bats that causes respiratory illnesses in humans. Micafungin (MCFG), an approved drug for treatment of fungal infections, has been shown to inhibit the propagation of PRV, but its precise mechanism of action remains unclear. In this study, we investigated the molecular mechanism of action of MCFG against PRV propagation. A molecular docking simulation showed that the p17 protein of PRV is likely to be the primary target of MCFG. Differential gene expression analysis was performed to compare MCFG-treated PRV-infected host cells with untreated infected cells, and IL-6 was found to be the main regulator induced by MCFG. Silencing of IL-6 using siRNA resulted in markedly increased levels of PRV release and syncytium formation and marginally increased PRV RNA replication. Treatment with an antibody against p17, the presumed target of MCFG, markedly reduced syncytium formation but did not influence viral RNA replication. In contrast, MCFG significantly suppressed syncytium formation and slightly reduced PRV RNA replication, and MCFG and anti-p17 antibody both increased IL-6 mRNA expression. Molecular docking analysis also suggested that MCFG might inhibit other PRV proteins, including the nonstructural replication protein σNS. In conclusion, it is likely that MCFG primarily targets p17 and modulates host immunity through IL-6, which probably interferes directly with syncytium formation.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-025-06369-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Full genome characterization of a novel zetapartitivirus infecting Alternaria tenuissima strain TY-26 associated with muskmelon leaf spot 侵染甜瓜叶斑病的黄斑互花菌TY-26株新病毒的全基因组鉴定

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-08-22 DOI: 10.1007/s00705-025-06388-1

Tongyu Mu, Xiaofan Sun, Wen Huang, Can Zhao, Xuehong Wu

{"title":"Full genome characterization of a novel zetapartitivirus infecting Alternaria tenuissima strain TY-26 associated with muskmelon leaf spot","authors":"Tongyu Mu, Xiaofan Sun, Wen Huang, Can Zhao, Xuehong Wu","doi":"10.1007/s00705-025-06388-1","DOIUrl":"10.1007/s00705-025-06388-1","url":null,"abstract":"<div><p>In China, leaf spot disease poses a significant challenge to muskmelon cultivation, with <i>Alternaria tenuissima</i> being identified as the predominant causal pathogen of this disease. In the current study, we identified and characterized a novel zetapartitivirus from <i>A</i>. <i>tenuissima</i> strain TY-26, which was named \"Alternaria tenuissima partitivirus 3\" (AttPV3). The full genome of AttPV3, the nucleotide sequence of which was determined by metatranscriptome sequencing and RNA-ligase-mediated rapid amplification of cDNA ends (RLM-RACE), comprises two dsRNA segments. The larger dsRNA1 segment is 1,841 nt in length and encodes a putative RNA-dependent RNA polymerase (RdRp), whereas the smaller dsRNA2 segment is 1,644 nt in length and encodes a putative capsid protein (CP). BLASTp searches showed that the RdRp and CP of AttPV3 had the highest amino acid (aa) sequence similarity to the corresponding proteins of Delitschia confertaspora partitivirus 1 (DcPV1), with 90.72% and 76.65% identity, respectively. Phylogenetic analysis of the RdRp aa sequence positioned AttPV3 within a clade alongside DcPV1, indicating its close evolutionary relationship to members of the the proposed genus “<i>Zetapartitivirus</i>” of the family <i>Partitiviridae</i>. This is the first report describing the full genomic characterization of a zetapartitivirus infecting <i>A. tenuissima</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144888124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0