Archives of Virology最新文献_第2页

Complete genome sequence and genome organization of Ficus pumila-associated virus, a new putative member of the family Tymoviridae 小叶榕相关病毒的全基因组序列和基因组组织——一种被推测为小叶榕科新成员的病毒

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-21 DOI: 10.1007/s00705-025-06312-7

Mengze Guo, Pingping Sun, Xudong Fan, Jianwei Guo, Lei Zhang, Zhengnan Li

引用次数: 0

Distinct effects of mucin on phage-host interactions in model systems of beneficial and pathogenic bacteria 黏液蛋白对有益菌和致病菌模型系统中噬菌体-宿主相互作用的独特影响

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-20 DOI: 10.1007/s00705-025-06322-5

Jaka Jakin Lazar, Katarina Šimunović, Iztok Dogša, Ines Mandić Mulec, Mathias Middelboe, Anna Dragoš

{"title":"Distinct effects of mucin on phage-host interactions in model systems of beneficial and pathogenic bacteria","authors":"Jaka Jakin Lazar, Katarina Šimunović, Iztok Dogša, Ines Mandić Mulec, Mathias Middelboe, Anna Dragoš","doi":"10.1007/s00705-025-06322-5","DOIUrl":"10.1007/s00705-025-06322-5","url":null,"abstract":"<div>Phage-host interactions that occur in host-associated microbiomes are influenced by a plethora of environmental factors. Mucins are glycoproteins that represent the main component of mucus, which is found in the animal digestive tract and on the surface of certain organs, serving as the first line of defense against toxins and pathogens. Previous studies have shown that lytic phages have an important influence on the microbial composition in mucosal areas. Our study expands this knowledge to interactions between previously untested lytic phages targeting probiotic and pathogenic bacteria, as well as temperate phages targeting probiotic bacteria. These interactions could be important in shaping microbial communities and affecting the well-being of their host. This study demonstrates that mucins enhance the adherence of Vibrio anguillarum lytic phages and Bacillus subtilis lytic and temperate phages, as well as B. subtilis and V. anguillarum cells, to solid surfaces. Our results also show that mucins positively affect the attachment of B. subtilis cells even in the presence of phages. This positive effect was not observed in the case of V. anguillarum. This suggests that mucin may shield certain bacteria from phage infections. We also found that mucin influenced the metabolic activity of the two tested bacterial species differently, with strong positive effects on V. anguillarum but not on B. subtilis. This work supports previous findings that phages adhere efficiently to mucus and extends these studies to include other beneficial and pathogenic bacterial species. It also reveals that mucins have different effects on phage-host interactions in different phage-host systems, which may have implications for phage therapies or probiotic treatment strategies.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-025-06322-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic characterization of the novel bacteriophage PfAn1 from Lake Baikal, infecting Pseudomonas fluorescens 贝加尔湖感染荧光假单胞菌的新型噬菌体PfAn1的基因组特征

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-16 DOI: 10.1007/s00705-025-06315-4

Anna Gorshkova, Olga Belykh, Irina Tikhonova, Li Xi, Maria Siniagina, Valentin Drucker, Sergey Potapov

引用次数: 0

Epidemiological investigation, isolation, and pathogenicity of porcine epidemic diarrhea virus subtype G2c in Sichuan province 猪流行性腹泻病毒G2c亚型在四川省的流行病学调查、分离及致病性

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-16 DOI: 10.1007/s00705-025-06308-3

Min Wang, Mingxiang Li, Guangwen Yan, Hao Li, Jun Zhou, Aiguo Yang

{"title":"Epidemiological investigation, isolation, and pathogenicity of porcine epidemic diarrhea virus subtype G2c in Sichuan province","authors":"Min Wang, Mingxiang Li, Guangwen Yan, Hao Li, Jun Zhou, Aiguo Yang","doi":"10.1007/s00705-025-06308-3","DOIUrl":"10.1007/s00705-025-06308-3","url":null,"abstract":"<div>Continued outbreaks of porcine epidemic diarrhea are causing serious economic losses to the swine industry in China. To monitor the prevalence, genetic mutations, and pathogenicity of porcine epidemic diarrhea virus (PEDV), 172 samples were collected from eight cities in Sichuan Province from 2020 to 2022. RT-PCR analysis revealed that 25.0% (43/172) of samples were positive for PEDV. Phylogenetic analysis of 17 S gene sequences (encoding the spike protein) showed that G2c was the main genotype circulating in Sichuan. One strain, Leshan-s-2020 (G2a), was identified as a recombinant resulting from inter-lineage recombination between the KM609212/LYG/2015 (G2a) and MianYang-s-2020 (G2a) strains in the S2 domain. In addition, the G2c strain YB2201, which was highly virulent in 4-day-old piglets, was successfully isolated. The results of this study enrich our understanding of the epidemiology of PEDV, the genetic characteristics and pathogenicity of the PEDV strains circulating in China, and the role of recombination in their evolution. These findings may contribute to the development of antigen detection reagents and vaccines.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of in vitro growth and pathogenicity in mice of intra-genotype chimeric strains of genotype IV Japanese encephalitis virus 乙型脑炎病毒基因型内嵌合株在小鼠体内的体外生长和致病性评价

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-16 DOI: 10.1007/s00705-025-06317-2

Shigeru Tajima, Astri Nur Faizah, Daisuke Kobayashi, Kris Cahyo Mulyatno, Sri Subekti, Maria Inge Lusida, Yasuko Mori, Haruhiko Isawa, Kyoko Sawabe, Chang-Kweng Lim

引用次数: 0

Complete genome sequence and characterisation of a novel flexivirus infecting the necrotrophic conifer pathogen Diplodia sapinea 一种感染坏死性针叶树病菌的新型弯曲病毒的全基因组序列和特征

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-16 DOI: 10.1007/s00705-025-06313-6

Suvi Sutela, Miloš Trifković, Eeva Terhonen

{"title":"Complete genome sequence and characterisation of a novel flexivirus infecting the necrotrophic conifer pathogen Diplodia sapinea","authors":"Suvi Sutela, Miloš Trifković, Eeva Terhonen","doi":"10.1007/s00705-025-06313-6","DOIUrl":"10.1007/s00705-025-06313-6","url":null,"abstract":"<div>Using total RNA sequencing, we detected a novel RNA virus infecting a Finnish isolate of the fungal conifer pathogen Diplodia sapinea, and its genome sequence was verified by Sanger sequencing. This virus, which we have named \"Diplodia sapinea flexi-like virus 1\" (DsFLV1), has a 7,494-nucleotide-long positive-sense single-stranded RNA genome, excluding the poly(A) tail. The largest open reading frame (ORF1) encodes a replication-associated polyprotein (RP) that contains conserved domains characteristic of members of the order Tymovirales, including viral methyltransferase (Mtr), viral helicase (Hel), and RNA-dependent RNA polymerase (RdRP) domains. Four smaller ORFs were also identified. ORF2 potentially encodes a capsid protein, while the other three ORFs, initiated by alternative start codons, encode putative proteins with unknown functions. Phylogenetic analysis placed DsFLV1 in a distinct clade within the family Deltaflexiviridae, close to Erysiphe necator-associated flexivirus 2. This is the first report of a positive-sense single-stranded RNA virus infecting D. sapinea.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-025-06313-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a virulent phage, P12L (genus Drulisvirus), targeting Klebsiella pneumoniae capsule type K2 针对肺炎克雷伯菌胶囊型K2的强毒噬菌体P12L （Drulisvirus属）的鉴定

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-16 DOI: 10.1007/s00705-025-06320-7

Wendy Dayang Rollon, Tay Darren Dean, Sharifah Ishaziah Mohd Idris, Nurzafirah Mazlan, Afif Pranaya Jati, Tze Young Thung

引用次数: 0

Molecular characterization of a novel mitovirus from the edible fungus Pleurotus pulmonarius 一种新型食用菌肺侧耳有丝分裂病毒的分子特征

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-16 DOI: 10.1007/s00705-025-06310-9

Zhe Wang, Junbo Xiao, Hanzhao Liu, Ziru Zheng, Yifei Zhang, Yingying Liu, Pengfei Li

引用次数: 0

Identification of pattern recognition receptors participating in the innate immune response to Tembusu virus 参与坦布苏病毒先天免疫应答的模式识别受体的鉴定

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-15 DOI: 10.1007/s00705-025-06307-4

Jingjing Huang, Xiaoyang Hu, Duo Peng, Qiong Li, Yinuo Song, Dabing Zhang

引用次数: 0

Association of grapevine geminivirus a isolate QN with upward leaf curling, necrosis, chlorosis, and malformation symptoms in two grapevine cultivars 葡萄双叶病毒a分离QN与两个葡萄品种向上卷曲、坏死、褪绿和畸形症状的关系

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-15 DOI: 10.1007/s00705-025-06318-1

Can Liu, Jinying Wang, Shangzhen Yu, Yinshuai Xie, Hanwei Li, Chenlu Feng, Mengqi Ma, Yixuan Wang, Yuqin Cheng

{"title":"Association of grapevine geminivirus a isolate QN with upward leaf curling, necrosis, chlorosis, and malformation symptoms in two grapevine cultivars","authors":"Can Liu, Jinying Wang, Shangzhen Yu, Yinshuai Xie, Hanwei Li, Chenlu Feng, Mengqi Ma, Yixuan Wang, Yuqin Cheng","doi":"10.1007/s00705-025-06318-1","DOIUrl":"10.1007/s00705-025-06318-1","url":null,"abstract":"<div>Grapevine geminivirus A (GGVA) is a recently identified DNA virus that infects grapevine, but its pathogenicity is not well studied. Previously, we investigated the pathogenicity of the new GGVA isolate QN (GGVAQN) on grapevine ‘Red Globe’ plants. In this study, grapevine ‘Red Globe’ and ‘Summer Black’ plants were inoculated with GGVAQN and grown without artificial control of temperature and light, and all of them exhibited upward leaf curling. This symptom was also found in field-grown grapevine ‘Shine Muscat’ plants infected with multiple grapevine viruses, including GGVA (isolate SM) (2,905 nt, sharing 97.69% genome sequence identity with GGVAQN), and in the upper developed leaves of previously GGVAQN-inoculated ‘Red Globe’ plants one week after they were moved from artificial to natural lighting. The upward leaf curling symptom was similar to that observed previously in Nicotiana benthamiana plants inoculated with GGVAQN, in field-grown grapevine ‘Queen Nina’ plants, and in in vitro-grown grapevine ‘Marselan’ plantlets, from which GGVAQN and GGVAML isolates with identical genome sequences were obtained. In addition to upward leaf curling, malformation, chlorosis, and necrosis symptoms were also observed on all GGVAQN-inoculated ‘Red Globe’ and ‘Summer Black’ plants. The findings presented here suggest that GGVAQN is closely associated with severe foliar symptoms in grapevine ‘Red Globe’ and ‘Summer Black’ cultivars and emphasize the need to control the virus.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0