Archives of Virology最新文献_第2页

Avian influenza A(H5N6) virus detected during live-poultry market surveillance linked to a human infection in Changsha, China, from 2020 to 2023

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-04-09 DOI: 10.1007/s00705-025-06280-y

Zheng Huang, Rusheng Zhang, Dong Yao, Huiyuan Fu, Lingzhi Li, Shan Xiao, Xinhua Ou, Mingzhong Xu, Qing-Bin Lu, Xuewen Yang

{"title":"Avian influenza A(H5N6) virus detected during live-poultry market surveillance linked to a human infection in Changsha, China, from 2020 to 2023","authors":"Zheng Huang, Rusheng Zhang, Dong Yao, Huiyuan Fu, Lingzhi Li, Shan Xiao, Xinhua Ou, Mingzhong Xu, Qing-Bin Lu, Xuewen Yang","doi":"10.1007/s00705-025-06280-y","DOIUrl":"10.1007/s00705-025-06280-y","url":null,"abstract":"<div><p>In November 2022, we reported a fatal case of human infection caused by a highly pathogenic avian influenza A(H5N6) virus bearing a clade 2.3.4.4b HA gene in Changsha City. We investigated the transmission route and distribution of the H5N6 virus in the largest live-poultry market (LPM), which is linked to the human infection. A total of 1357 samples from the LPM were collected for avian influenza A virus detection from 2020 to 2023. The proportion of LPM samples positive for H5 subtype avian influenza virus was 14.30% (194/1357). Sequences of H5N6 (n = 10) and H5N1 (n = 4) avian influenza viruses were obtained from the LPM samples using next-generation sequencing. The complete genome sequence of the H5N6 virus from the human infection case, A/Changsha/1/2022(EPI_ISL_16466440), was determined and analyzed. The PB1 and PB2 segments shared 99.65% and 99.23% sequence identity with A/duck/Hunan/S40199/2021(H5N6) and A/Whooper swan/Sanmenxia/H615/2020(H5N8), respectively. The other segments showed the highest sequence similarity to strain A/Guangdong/1/2021(H5N6), which was isolated in Guangzhou. L89V and I292V substitutions in the PB2 protein were predicted from the A/Changsha/1/2022 genome sequence. Phylogenetic analysis based on the HA gene showed that A/Changsha/1/2022 and other H5 subtype isolates obtained from the LPM grouped together in the 2.3.4.4b branch. Bayesian evolutionary analysis of the HA gene showed that clade 2.3.4.4b of the H5N6 virus is likely to have been prevalent in Hunan Province around October 2021. In conclusion, we confirmed that the clade 2.3.4.4b HA gene of A/Changsha/1/2022 virus recombined with those of local strains. These results demonstrate the importance of continuous surveillance of H5N6 influenza viruses.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porcine circovirus type 2 (PCV2) in Brazil in the past 55 years (1967-2022): evolutionary rates and patterns

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-04-09 DOI: 10.1007/s00705-025-06276-8

I. L. F. Rodrigues, A. C. M. Cruz, A. E. Souza, F. B. Knackfuss, C. H. C. Costa, B. V. Lago, R. L. Silveira, T. X. Castro

{"title":"Porcine circovirus type 2 (PCV2) in Brazil in the past 55 years (1967-2022): evolutionary rates and patterns","authors":"I. L. F. Rodrigues, A. C. M. Cruz, A. E. Souza, F. B. Knackfuss, C. H. C. Costa, B. V. Lago, R. L. Silveira, T. X. Castro","doi":"10.1007/s00705-025-06276-8","DOIUrl":"10.1007/s00705-025-06276-8","url":null,"abstract":"<div><p>Porcine circovirus type 2 (PCV2) is a common virus that infects pigs. It exhibits marked genetic variability, which is affected by factors such as vaccination and commercial trade. In this study, a retrospective analysis of PCV2 was performed based on molecular characterization, Bayesian inference, and haplotype networks in tissue samples obtained from Brazilian pig herds from 1967 to 2018. A nested PCR targeting a 225-nucleotide fragment of the PCV2 capsid gene was performed, followed by bidirectional sequencing. Out of 178 samples tested, 39 (21.9%) were positive for PCV2. Phylogenetic analysis showed that strains from this study collected before the introduction of a PCV2 vaccine clustered with genotype 2d reference strains. The mean substitution rate was 1.54 × 10<sup>-3</sup> substitutions per site per year (s/s/y). The haplotype diversity of PCV2 was 0.98, which shows a high mutation rate compatible with the topology found in the haplotype network. Our study shows that most of the Brazilian isolates, including those obtained before the introduction of the PCV2 vaccine, were similar to PCV2d reference strains, suggesting that this genotype was already circulating before the introduction of the vaccine in Brazil. One of the PCV2 sequences was from an isolate from 1967, making it the oldest sequenced isolate from the Americas, and the second oldest worldwide. Phylogenetic analysis and haplotype network analysis indicated a high degree of diversity of PCV2 as well as a high rate of evolution. These results increase our knowledge about the genetic diversity, evolution, and dispersal patterns of PCV2 in Brazil.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a recombinant GII.13[P21] norovirus strain: molecular dynamic simulations indicate that gene mutations shifted its spectrum of binding to host receptor glycans

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-04-01 DOI: 10.1007/s00705-025-06277-7

Yunfei Chen, Zexian Zhou, Lei Dong, Miao Jin, Yongjie Wang, Yongxin Yu

引用次数: 0

Complete genome sequences of two different broad bean stain virus isolates

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-04-01 DOI: 10.1007/s00705-025-06273-x

Fumino Nito, Yuya Imamura, Yuji Fujiwara, Takayuki Matsuura, Hironobu Yanagisawa

引用次数: 0

Evolutionary analysis and population dynamics in the global transmission of Kaposi’s sarcoma-associated herpesvirus

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-27 DOI: 10.1007/s00705-025-06259-9

Tianye Wang, Jiaqi Wu, So Nakagawa, Takahiro Yonezawa, Zhenqiu Liu, Xin Zhang, Haili Wang, Yi Li, Tiejun Zhang

{"title":"Evolutionary analysis and population dynamics in the global transmission of Kaposi’s sarcoma-associated herpesvirus","authors":"Tianye Wang, Jiaqi Wu, So Nakagawa, Takahiro Yonezawa, Zhenqiu Liu, Xin Zhang, Haili Wang, Yi Li, Tiejun Zhang","doi":"10.1007/s00705-025-06259-9","DOIUrl":"10.1007/s00705-025-06259-9","url":null,"abstract":"<div><p>With the widespread prevalence of Kaposi’s sarcoma-associated herpesvirus (KSHV) globally, particularly in sub-Saharan Africa and the Mediterranean region, the open reading frame (ORF) K1 gene, a key gene for distinguishing different subtypes of KSHV, has been extensively studied for its diversity and sequence variations. In this study, we collected K1 gene sequences representing subtypes of KSHV worldwide in order to assess the global distribution of KSHV subtypes and to investigate the recombination and selection history of KSHV. Recombination and gene flow analysis indicated a minimum average recombination rate of 0.41 per site for the K1 gene. Recombination analysis indicated that 11 major recombination events had occurred, predominantly in subtypes A and C, while subtype B showed minimal involvement in recombination processes, consistent with the gene flow analysis. Using tip-dating methods, we estimated that the most recent common ancestor of KSHV emerged in the 12th century, while the currently globally prevalent subtypes appeared within the past three centuries. Its recent origin and rapid evolution indicate that KSHV is now undergoing strong selection and is in the process of adaptation.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-025-06259-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, identification, and genome analysis of the novel Escherichia coli phage XH12 and enhancement of the antibacterial activity of its lysozyme by chimeric cationic peptides

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-27 DOI: 10.1007/s00705-025-06274-w

Xuhao Hou, Jiaqi Pu, Yu Li, Wenhai Xie, Limei Zhang, Hongkuan Deng

{"title":"Isolation, identification, and genome analysis of the novel Escherichia coli phage XH12 and enhancement of the antibacterial activity of its lysozyme by chimeric cationic peptides","authors":"Xuhao Hou, Jiaqi Pu, Yu Li, Wenhai Xie, Limei Zhang, Hongkuan Deng","doi":"10.1007/s00705-025-06274-w","DOIUrl":"10.1007/s00705-025-06274-w","url":null,"abstract":"<div><p>Antibiotics are no longer adequate to address the threat of antibiotic resistance, especially in <i>Pseudomonas aeruginosa</i>, <i>Acinetobacter baumannii</i>, <i>Escherichia coli</i>, and other Gram-negative pathogens that pose a serious threat to human health worldwide. The antibiotic resistance pandemic has brought about a need to search for new antimicrobials as alternatives that are effective and less prone to resistance. Phages and their lysozymes have become an attractive alternative to currently available antibiotics. However, Gram-negative bacteria have an outer membrane that acts as a strong barrier, so lysozymes are often used in combination with an outer membrane permeator or are modified to overcome the outer membrane barrier. To combat drug-resistant <i>E. coli</i>, in this study, we used the multidrug-resistant <i>E. coli</i> isolate Eco-3 as a host to isolate a lytic phage, XH12, from sewage. Phage XH12 was found to lyse 81% (30/37) of the <i>E. coli</i> isolates tested. The biological characteristics and genome sequence of phage XH12 were analyzed, and we found that lysozyme lys12 encoded by phage XH12, when combined with ethylenediaminetetraacetic acid (EDTA), exhibited antibacterial activity against <i>E. coli</i>. Two modified lysozymes were obtained by fusing cationic amino acid polypeptides to the C-terminus of lys12. The fusion lysozymes increased the antibacterial activity against <i>E. coli</i> in the extracellular space. This study of phage XH12 and its lysozyme provides basic information for further study of the treatment of multidrug-resistant <i>E. coli</i> infections.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome sequence of a novel mitovirus identified in the phytopathogenic fungus Puccinia triticina

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-27 DOI: 10.1007/s00705-025-06272-y

Yanhui Zhang, Nuoheng Wang, Jinyang Li, Bingtao Chen, Zhensheng Kang, Pengyu Song, Wenming Zheng

引用次数: 0

Exploring evidence from cells to clinics: is human bocavirus a gastrointestinal pathogen or just a risk factor?

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-24 DOI: 10.1007/s00705-025-06265-x

Mehrdad Mohammadi

{"title":"Exploring evidence from cells to clinics: is human bocavirus a gastrointestinal pathogen or just a risk factor?","authors":"Mehrdad Mohammadi","doi":"10.1007/s00705-025-06265-x","DOIUrl":"10.1007/s00705-025-06265-x","url":null,"abstract":"<div><p>Human bocaviruses (HBoVs), first identified in 2005 and composed of genotypes 1–4, have been increasingly detected worldwide in pediatric patients with acute gastroenteritis. HBoV-1 has been primarily associated with respiratory symptoms, while HBoV2-4 are mostly found in gastrointestinal (GI) samples. Results from case-control studies are still controversial; however, epidemiological evidence has shown a significant association between HBoV-2 and gastroenteritis. This review will primarily focus on this association, with a brief discussion of evidence related to other HBoV genotypes. Pathological and molecular studies on the pathogenesis of HBoV, particularly in GI cells, are very scarce, possibly due to the difficulties of <i>in vitro</i> HBoV culture. Nonetheless, some relevant findings from colorectal cancer samples have yielded valuable insights regarding the behavior of HBoV in the GI system. In the present review, we provide an updated overview of the epidemiological evidence for an association of HBoV infection with acute gastroenteritis and focus on the cellular and molecular perspectives of HBoV pathogenicity. Finally, we look at the knowledge gaps about how HBoV affects the GI system and explore future directions.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and biological characterization of African swine fever virus clones selected at the early stages of adaptation in Vero cells

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-24 DOI: 10.1007/s00705-025-06269-7

Tae-Young Suh, Ji-Hoon Park, Chae-Rin Park, Ji-Eun Kim, Jun Young Park, Seong Yun Hwang, Yong-Joo Kim, Hae-Eun Kang, Dae-Yong Kim, Jun-Gu Choi

{"title":"Genetic and biological characterization of African swine fever virus clones selected at the early stages of adaptation in Vero cells","authors":"Tae-Young Suh, Ji-Hoon Park, Chae-Rin Park, Ji-Eun Kim, Jun Young Park, Seong Yun Hwang, Yong-Joo Kim, Hae-Eun Kang, Dae-Yong Kim, Jun-Gu Choi","doi":"10.1007/s00705-025-06269-7","DOIUrl":"10.1007/s00705-025-06269-7","url":null,"abstract":"<div><p>African swine fever is a highly contagious viral disease affecting domestic pigs and wild boars, caused by African swine fever virus (ASFV). ASFV exhibits highly restricted cell tropism, primarily targeting porcine macrophages for efficient and stable replication. This strict requirement for a specific cell type has posed significant challenges in ASFV research and remains a critical obstacle to vaccine development. Although several cell lines have been reported to support ASFV replication, significant genetic changes occur during passages in cell lines in unpredictable ways. In this study, we adapted a highly virulent Korean ASFV isolate to Vero cells, and plaque purification was employed during the early stages of cell adaptation to eliminate the competitive pressure between viral subpopulations. Three viral clones with distinct genetic properties were obtained and passaged independently up to 30 times. The three clones replicated efficiently in porcine alveolar macrophages (PAMs) and Vero cells even after 30 passages, while the parent virus did not grow in Vero cells. Genetic analysis revealed that the cell-adapted viral clones shared multiple single-nucleotide polymorphisms (SNPs) but also had unique SNPs and large deletions in their genome. This diversity of mutations in these clones could provide a valuable model for exploring gene functions and their effects on phenotypic properties. Future studies will focus on evaluating the phenotypic effects of these SNPs <i>in vivo</i> to support vaccine development.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of two novel bacteriophages of the class Caudoviricetes that infect Ralstonia solanacearum and Ralstonia pseudosolanacearum

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-24 DOI: 10.1007/s00705-025-06271-z

Beatriz Dias Januário, Rafael Reis de Rezende, Tulio Morgan, Poliane Alfenas-Zerbini

引用次数: 0