Archives of Virology最新文献_第3页

ACE2, a therapeutic target of COVID-19, needs to be treated with caution. ACE2是COVID-19的治疗靶点，需要谨慎对待。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-29 DOI: 10.1007/s00705-025-06327-0

Xiang'e Liu

{"title":"ACE2, a therapeutic target of COVID-19, needs to be treated with caution.","authors":"Xiang'e Liu","doi":"10.1007/s00705-025-06327-0","DOIUrl":"https://doi.org/10.1007/s00705-025-06327-0","url":null,"abstract":"<p><p>Angiotensin-converting enzyme 2 (ACE2) has garnered significant attention for its crucial role in infection by both SARS-CoV and SARS-CoV-2. Consequently, it has emerged as a potential therapeutic target for treatment of COVID-19. It is therefore important to understand the mechanisms and modes of action of current and future treatments involving ACE2. Three important strategies have been explored in previous studies: (1) interruption of the interaction between ACE2 and the coronavirus spike protein using compounds or monoclonal antibodies, (2) capturing the extracellular virus by employing soluble ACE2 as a decoy, and (3) reducing the expression or inhibiting the activity of ACE2 through genetic approaches or drug intervention. However, the third strategy of inhibiting ACE2 activity as a means of treating COVID-19 is potentially risky, and the wisdom of pursuing this approach is subject to debate. Here, the advisability of using anti-ACE2 treatment in the context of SARS-CoV and SARS-CoV-2 infections is challenged by reviewing the physiological function of ACE2 and the mechanism of viral entry, emphasizing the pathological impairment of ACE2 that occurs during SARS-CoV and SARS-CoV-2 infection and arguing that the potential hazards associated with ACE2 impairment should be given more attention. Because of the important concerns regarding the potential side effects of ACE2 inhibition, researchers are strongly urged to approach this issue with caution.</p>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"143"},"PeriodicalIF":2.5,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploiting the chikungunya virus capsid protein: a focused target for antiviral therapeutic development 基孔肯雅病毒衣壳蛋白的开发：抗病毒治疗开发的重点靶点

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-27 DOI: 10.1007/s00705-025-06325-2

Kavitha Karunakaran, Abdul Ajees Abdul Salam, Piya Paul Mudgal

{"title":"Exploiting the chikungunya virus capsid protein: a focused target for antiviral therapeutic development","authors":"Kavitha Karunakaran, Abdul Ajees Abdul Salam, Piya Paul Mudgal","doi":"10.1007/s00705-025-06325-2","DOIUrl":"10.1007/s00705-025-06325-2","url":null,"abstract":"<div><p>Chikungunya disease is spread by the bite of infected <i>Aedes</i> mosquitoes. It is considered a neglected tropical disease that has the potential to cause sporadic epidemics in naive populations. Despite the substantial investment in research, there are no approved antiviral treatments for chikungunya. Several screening approaches have been used to identify potential antiviral molecules that target the whole virus, viral proteins, and viral-host interactions, often in conjunction with computational studies. The genome of chikungunya virus (CHIKV) encodes four nonstructural and five structural proteins. The capsid protein (CP) is a small structural protein with enzymatic activity. Owing to its critical role in different stages of the viral life cycle, the CP can be targeted at multiple stages, thereby impeding viral multiplication. There is evidence suggesting that the CP may be a promising target for drug development, and this has led to the discovery of various inhibitors through diverse in vitro and in silico analyses. Both cell-based and cell-free assays have been widely used to identify and evaluate CHIKV CP inhibitors. Computer-based studies targeting CHIKV proteins, including CP, have identified several lead compounds, which are being further evaluated in various in vitro systems. No review has been published on the CHIKV CP, and papers have focused on drug development and the targeting of viral proteins and associated factors. In this review, we summarize the research that has been conducted on the CHIKV CP, including structural studies, antiviral research, and prospects for the use of the CP as an antiviral target.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arboviruses: the hidden danger of the tropics 虫媒病毒：热带地区的潜在危险

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-26 DOI: 10.1007/s00705-025-06314-5

Zhen Yun Siew, Isaac Seow, Xin Rui Lim, Chen Zhe Tang, Fadhilah Moh Djamil, Ghee Khang Ong, Pooi Pooi Leong, Siew Tung Wong, Kenny Voon

引用次数: 0

The biomechanical phenomena observed in the cell invasion pathway of porcine epidemic diarrhea virus: a review 猪流行性腹泻病毒细胞侵袭途径的生物力学现象综述

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-26 DOI: 10.1007/s00705-025-06326-1

Hong Zou, Yi Wang, Gan Luo, Shilei Huang

{"title":"The biomechanical phenomena observed in the cell invasion pathway of porcine epidemic diarrhea virus: a review","authors":"Hong Zou, Yi Wang, Gan Luo, Shilei Huang","doi":"10.1007/s00705-025-06326-1","DOIUrl":"10.1007/s00705-025-06326-1","url":null,"abstract":"<div><p>Porcine epidemic diarrhea virus (PEDV) is the primary pathogen responsible for highly contagious intestinal infections in pigs, which results in significant economic losses to the global animal husbandry industry. PEDV is an enveloped virus that enters cells via endocytosis, a process that is dependent on the binding of the viral surface S protein to a receptor on the host cell membrane. This results in a series of biomechanical alterations that drive the fusion of the viral and host cell membranes. These alterations stabilise the binding of the virus to the receptor and also affect the tension and the curvature of the plasma membrane and the formation of endocytic vesicles. A comprehensive understanding of the mechanism by which PEDV enters cells and the biomechanical changes that accompany this process is of paramount importance for the development of PEDV inhibitors, vaccines, and disease prevention and control strategies. Here, we review the general mechanism of PEDV entry, the biomechanical phenomena that occur during endocytosis, and the potential applications of biomechanics in antiviral therapy. It is anticipated that by gaining insight into these mechanisms, novel approaches to regulating viral entry pathways through mechanical interference, vaccine development, and antiviral drug design can be explored.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a novel phenuivirus with an unusual ambisense genome from the ascomycete fungus Fusarium fujikuroi 从子囊菌真菌fujikuroi镰刀菌中鉴定出一种具有不寻常双义基因组的新型猴病毒

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-26 DOI: 10.1007/s00705-025-06311-8

Tongle Xin, Bo Liu, Hongqian Liu, Ziqi Wang, Junmin Li, Shengqi Chi, Ida Bagus Andika, Xinran Cao

{"title":"Identification of a novel phenuivirus with an unusual ambisense genome from the ascomycete fungus Fusarium fujikuroi","authors":"Tongle Xin, Bo Liu, Hongqian Liu, Ziqi Wang, Junmin Li, Shengqi Chi, Ida Bagus Andika, Xinran Cao","doi":"10.1007/s00705-025-06311-8","DOIUrl":"10.1007/s00705-025-06311-8","url":null,"abstract":"<div><p>A novel virus, tentatively named “Fusarium fujikuroi negative-strand RNA virus 1” (FfNSRV1), was identified in a <i>Fusarium fujikuroi</i> strain isolated from a small brown planthopper. The FfNSRV1 genome consists of three negative-sense, single-stranded RNA segments (RNA1-3) with lengths of 6649, 1609, and 1380 nt, respectively. The viral complementary (vc) strand (positive sense) of RNA1 encodes a large protein (∼252 kDa) containing a conserved domain of the bunyavirus RNA-dependent RNA polymerase (RdRP) superfamily, with the highest sequence similarity (40-44% identity) to the RdRPs encoded by established members of the genus <i>Coguvirus</i> in the family <i>Phenuiviridae</i>. The RNA2 vc strand encodes a protein (∼54 kDa) showing sequence similarity (38-40% identity) to the movement protein-like (MP-L) proteins of coguviruses. The RNA3 vc strand encodes a protein (∼44 kDa) with a conserved domain of the phenuivirid nucleocapsid protein superfamily. Interestingly, the RNA2 and RNA3 segments (negative sense) each also contain an open reading frame (ORF) that overlaps with the ORF in the vc strand. The protein encoded by the RNA2 negative-strand ORF shows a low degree of sequence similarity (23-30% identity) to the MP-L proteins of unassigned phenuiviruses, and the protein encoded by the RNA3 negative-strand ORF shows a low degree of similarity (26-29% identity) to the nucleocapsid proteins of established members of the genus <i>Bocivirus</i> in the family <i>Phenuiviridae</i>. Phylogenetic analysis based on RdRP sequences showed that FfNSRV1 clustered with coguviruses, but in a separate monophyletic clade. Our results suggest that FfNSRV1 should be placed in a new genus within the family <i>Phenuiviridae</i> due to its unusual tripartite ambisense genome organization.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and epidemiological insights into serotypes of foot-and-mouth disease virus in Pakistan 巴基斯坦口蹄疫病毒血清型的分子特征和流行病学见解

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-25 DOI: 10.1007/s00705-025-06319-0

Syeda Anam Masood Gardezi, Masood Rabbani, Muhammad Hassan Mushtaq, Muhammad Zubair Shabbir

{"title":"Molecular characterization and epidemiological insights into serotypes of foot-and-mouth disease virus in Pakistan","authors":"Syeda Anam Masood Gardezi, Masood Rabbani, Muhammad Hassan Mushtaq, Muhammad Zubair Shabbir","doi":"10.1007/s00705-025-06319-0","DOIUrl":"10.1007/s00705-025-06319-0","url":null,"abstract":"<div><p>Foot-and-mouth disease virus (FMDV) causes a highly infectious transboundary disease in cloven-hoofed animals. However, there is limited information on comparative genomic and evolutionary analysis of FMDV strains reported in Pakistan. In the current study, we investigated a few disease outbreaks in Pakistan and determined the complete genome sequences of three isolates belonging to different serotypes: O, A, and Asia-1. Comparative genomic analysis showed a close relationship between the FMDV strains from this study and those reported in neighboring Asian countries. Phylogenetic analysis based on the complete genome and VP1 gene sequences revealed that serotype O clustered within the Pak-98 lineage, serotype A within genotype I, and serotype Asia-1 within group 5, grouping with strains from Pakistan, India, and China. Potential negatively selected sites were identified in the region encoding the structural proteins VP4, VP3, VP2, and VP1, with the most episodic diversifying section observed in the VP1 and VP3 regions. Substantial variation was observed in polymorphic and monomorphic sites, with the highest diversity observed in the VP1 protein of serotype O viruses. No evidence of recombination events was found in the FMDV strains from this study. Our findings indicate the cocirculation of multiple serotypes and sublineages of FMDV in Pakistan, underscoring the potential for the emergence of new variants. This study adds to the existing knowledge on the genetic variation and evolutionary dynamics of FMDV serotypes in Pakistan, providing valuable insights for the development of effective vaccines and improved disease control strategies.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and complete genome sequence of a new carlavirus from Carica papaya in Jordan 约旦番木瓜新卡拉病毒的鉴定及全基因组序列分析

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-22 DOI: 10.1007/s00705-025-06309-2

N. Salem, P. Margaria, M. Abu Muslem, I. Ibrahim, A. Alawidat

引用次数: 0

MF59-like adjuvant containing yeast-derived squalene enhances the humoral immune response to cell-derived influenza vaccine 含有酵母衍生角鲨烯的mf59样佐剂增强了对细胞衍生流感疫苗的体液免疫应答

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-21 DOI: 10.1007/s00705-025-06306-5

Tuanjie Chen, Wenting Xu, Peng Duan, Sijing Jiang, Xue Yang, Hongzhen Cao, Mei Zheng, Jian Luo

{"title":"MF59-like adjuvant containing yeast-derived squalene enhances the humoral immune response to cell-derived influenza vaccine","authors":"Tuanjie Chen, Wenting Xu, Peng Duan, Sijing Jiang, Xue Yang, Hongzhen Cao, Mei Zheng, Jian Luo","doi":"10.1007/s00705-025-06306-5","DOIUrl":"10.1007/s00705-025-06306-5","url":null,"abstract":"<div><p>The aims of this study were to assess the adjuvant properties of an MF59-like adjuvant containing yeast-derived squalene (MF59-like YD) in a cell-based quadrivalent influenza vaccine (QIV) and to investigate the potential mechanisms of action. MF59-like adjuvants containing either yeast-derived or shark-derived squalene were incorporated into QIV formulations. Antigen-specific immune responses in mouse serum were evaluated via enzyme-linked immunosorbent assays (ELISAs), hemagglutination inhibition (HI) assays, and microneutralization (MN) assays. The effects and mechanisms of action of the adjuvants were further analyzed by analyzing mouse spleen germinal center (GC) cell activation via flow cytometry. MF59-like YD significantly increased the humoral immune responses induced by QIVs in mice, in particular, the titers of HI and MN antibodies against homologous and heterologous virus subtypes. Mechanistically, MF59-like YD increased the immune response to influenza vaccines by activating T follicular helper (Tfh) and B cells in the GC. Given the greater availability of yeast-derived squalene and the finding that its adjuvant efficacy was comparable to that of shark-derived squalene, we propose that the MF59-like YD adjuvant is a promising alternative adjuvant for future influenza vaccines.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome sequence and genome organization of Ficus pumila-associated virus, a new putative member of the family Tymoviridae 小叶榕相关病毒的全基因组序列和基因组组织——一种被推测为小叶榕科新成员的病毒

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-21 DOI: 10.1007/s00705-025-06312-7

Mengze Guo, Pingping Sun, Xudong Fan, Jianwei Guo, Lei Zhang, Zhengnan Li

引用次数: 0

Distinct effects of mucin on phage-host interactions in model systems of beneficial and pathogenic bacteria 黏液蛋白对有益菌和致病菌模型系统中噬菌体-宿主相互作用的独特影响

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-05-20 DOI: 10.1007/s00705-025-06322-5

Jaka Jakin Lazar, Katarina Šimunović, Iztok Dogša, Ines Mandić Mulec, Mathias Middelboe, Anna Dragoš

{"title":"Distinct effects of mucin on phage-host interactions in model systems of beneficial and pathogenic bacteria","authors":"Jaka Jakin Lazar, Katarina Šimunović, Iztok Dogša, Ines Mandić Mulec, Mathias Middelboe, Anna Dragoš","doi":"10.1007/s00705-025-06322-5","DOIUrl":"10.1007/s00705-025-06322-5","url":null,"abstract":"<div><p>Phage-host interactions that occur in host-associated microbiomes are influenced by a plethora of environmental factors. Mucins are glycoproteins that represent the main component of mucus, which is found in the animal digestive tract and on the surface of certain organs, serving as the first line of defense against toxins and pathogens. Previous studies have shown that lytic phages have an important influence on the microbial composition in mucosal areas. Our study expands this knowledge to interactions between previously untested lytic phages targeting probiotic and pathogenic bacteria, as well as temperate phages targeting probiotic bacteria. These interactions could be important in shaping microbial communities and affecting the well-being of their host. This study demonstrates that mucins enhance the adherence of <i>Vibrio anguillarum</i> lytic phages and <i>Bacillus subtilis</i> lytic and temperate phages, as well as <i>B. subtilis</i> and <i>V. anguillarum</i> cells, to solid surfaces. Our results also show that mucins positively affect the attachment of <i>B. subtilis</i> cells even in the presence of phages. This positive effect was not observed in the case of <i>V. anguillarum</i>. This suggests that mucin may shield certain bacteria from phage infections. We also found that mucin influenced the metabolic activity of the two tested bacterial species differently, with strong positive effects on <i>V. anguillarum</i> but not on <i>B. subtilis</i>. This work supports previous findings that phages adhere efficiently to mucus and extends these studies to include other beneficial and pathogenic bacterial species. It also reveals that mucins have different effects on phage-host interactions in different phage-host systems, which may have implications for phage therapies or probiotic treatment strategies.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-025-06322-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0