Timothy Martin, Liana Theodoridis, Emma Stent, Julia Lilla, Steve Petrovski
{"title":"肺炎克雷伯菌噬菌体vB_Kpn_Lilla1和vB_Kpn_Lilla2的分离与鉴定","authors":"Timothy Martin, Liana Theodoridis, Emma Stent, Julia Lilla, Steve Petrovski","doi":"10.1007/s00705-025-06374-7","DOIUrl":null,"url":null,"abstract":"<div><p><i>Klebsiella pneumoniae</i> is an opportunistic pathogen that is commonly associated with pulmonary infections. It is of particular concern due to its increasing resistance to antibiotics, particularly carbapenem. Alternative treatments for these bacterial infections are required, and phage application is an attractive option. In this study, we isolated two novel <i>Klebsiella pneumonia</i> bacteriophages from wastewater, vB_Kpn_Lilla1 and vB_Kpn_Lilla2, both of which have lytic activity against <i>Klebsiella oxytoca</i> and <i>Klebsiella michiganensis</i>. Genome sequencing and phylogenetic analysis revealed that both phages belong to the family <i>Straboviridae</i>, with vB_Kpn_Lilla1 belonging to the genus <i>Jiaodavirus</i> and vB_Kpn_Lilla2 belonging to the genus <i>Slopekvirus</i>. Growth kinetics showed an effective reduction of <i>K. pneumoniae</i> populations when infected with either phage.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 8","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12307530/pdf/","citationCount":"0","resultStr":"{\"title\":\"Isolation and characterisation of Klebsiella pneumoniae bacteriophages vB_Kpn_Lilla1 and vB_Kpn_Lilla2\",\"authors\":\"Timothy Martin, Liana Theodoridis, Emma Stent, Julia Lilla, Steve Petrovski\",\"doi\":\"10.1007/s00705-025-06374-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><i>Klebsiella pneumoniae</i> is an opportunistic pathogen that is commonly associated with pulmonary infections. It is of particular concern due to its increasing resistance to antibiotics, particularly carbapenem. Alternative treatments for these bacterial infections are required, and phage application is an attractive option. In this study, we isolated two novel <i>Klebsiella pneumonia</i> bacteriophages from wastewater, vB_Kpn_Lilla1 and vB_Kpn_Lilla2, both of which have lytic activity against <i>Klebsiella oxytoca</i> and <i>Klebsiella michiganensis</i>. Genome sequencing and phylogenetic analysis revealed that both phages belong to the family <i>Straboviridae</i>, with vB_Kpn_Lilla1 belonging to the genus <i>Jiaodavirus</i> and vB_Kpn_Lilla2 belonging to the genus <i>Slopekvirus</i>. Growth kinetics showed an effective reduction of <i>K. pneumoniae</i> populations when infected with either phage.</p></div>\",\"PeriodicalId\":8359,\"journal\":{\"name\":\"Archives of Virology\",\"volume\":\"170 8\",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-07-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12307530/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Virology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00705-025-06374-7\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VIROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Virology","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s00705-025-06374-7","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
Isolation and characterisation of Klebsiella pneumoniae bacteriophages vB_Kpn_Lilla1 and vB_Kpn_Lilla2
Klebsiella pneumoniae is an opportunistic pathogen that is commonly associated with pulmonary infections. It is of particular concern due to its increasing resistance to antibiotics, particularly carbapenem. Alternative treatments for these bacterial infections are required, and phage application is an attractive option. In this study, we isolated two novel Klebsiella pneumonia bacteriophages from wastewater, vB_Kpn_Lilla1 and vB_Kpn_Lilla2, both of which have lytic activity against Klebsiella oxytoca and Klebsiella michiganensis. Genome sequencing and phylogenetic analysis revealed that both phages belong to the family Straboviridae, with vB_Kpn_Lilla1 belonging to the genus Jiaodavirus and vB_Kpn_Lilla2 belonging to the genus Slopekvirus. Growth kinetics showed an effective reduction of K. pneumoniae populations when infected with either phage.
期刊介绍：
Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
