Archives of Virology最新文献_第4页

Complete genome analysis of a novel phenui-like virus in Morus alba 一种新的白桑猴样病毒的全基因组分析。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-07-29 DOI: 10.1007/s00705-025-06378-3

Hanze Zheng, Yangwei Gao, Xiaodi Hu, Zhongtian Xu, Jian-Ping Chen, Yiyuan Li

{"title":"Complete genome analysis of a novel phenui-like virus in Morus alba","authors":"Hanze Zheng, Yangwei Gao, Xiaodi Hu, Zhongtian Xu, Jian-Ping Chen, Yiyuan Li","doi":"10.1007/s00705-025-06378-3","DOIUrl":"10.1007/s00705-025-06378-3","url":null,"abstract":"<div><p>Mulberries (genus <i>Morus</i>) are important to sericulture. Due to interspecific hybridization and the existence of various cultivars, the virome of many <i>Morus</i> species is still unknown. In this study, we identified a novel negative-sense phenui-like virus in <i>Morus alba</i> and determined its genome sequence. The virus possesses three linear single-stranded negative-sense RNA segments: RNA1 (7,329 nt), RNA2 (1,276 nt), and RNA3 (1,222 nt). Each of the three segments contains an open reading frame (ORF), encoding the RNA-dependent RNA polymerase (RdRP), the movement protein (MP), and the nucleocapsid protein (N), respectively. Phylogenetic analysis placed this virus within a clade including members of the genus <i>Rubodvirus</i> (family <i>Phenuiviridae</i>, order <i>Hareavirales</i>, class <i>Bunyaviricetes</i>) with raspberry rubodvirus 1, grapevine Muscat rose virus, and apple rubbery wood virus 2 being the closest relatives. Small interfering RNA (siRNA) sequencing data suggested that the virus replicates in <i>Morus alba</i>. Our study provides the first report of a rubodvirus in <i>Morus alba</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological characterization and genomic profiling of a novel Providencia phage isolated from farm effluents 从农场废水中分离的一种新型普罗维登斯噬菌体的生物学特性和基因组图谱。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-07-29 DOI: 10.1007/s00705-025-06373-8

Mantong Zhu, Chang Liu, Guangfeng Liu, Hongsai Zhang, Xing Wang, Ying Wang, Yongqi Luo, Jingzhe Jiang

{"title":"Biological characterization and genomic profiling of a novel Providencia phage isolated from farm effluents","authors":"Mantong Zhu, Chang Liu, Guangfeng Liu, Hongsai Zhang, Xing Wang, Ying Wang, Yongqi Luo, Jingzhe Jiang","doi":"10.1007/s00705-025-06373-8","DOIUrl":"10.1007/s00705-025-06373-8","url":null,"abstract":"<div><p>A <i>Providencia</i> strain was isolated from farm effluents and used as a host to isolate a bacteriophage named A06. A double-layer agar plaque assay was used to determine the optimal multiplicity of infection (MOI), construct a one-step growth curve, evaluate the temperature and pH stability of the phage, and assess its <i>in vitro</i> antibacterial activity and lysis efficiency. Whole-genome sequencing, functional annotation, and comparative genomics analysis were used to analyze its biological features and determine its taxonomic position. Bacteriophage A06 has a circular DNA genome with 58 open reading frames (ORFs). The optimal MOI was determined to be 100, and a one-step growth curve showed the latent period to be 40 minutes and the lysis period to range from 40 to 90 minutes. Bacteriophage A06 exhibited a high degree of thermal stability and pH tolerance, and genomic analysis indicated that it represents a new species within the genus <i>Kakivirus</i> of the family <i>Autoscriptoviridae</i>.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of phage CSF, a novel generalist bacteriophage targeting multidrug-resistant and potentially pathogenic Staphylococcus spp. and Mammaliicoccus spp. 噬菌体CSF的发现，一种针对多重耐药和潜在致病性葡萄球菌和哺乳球菌的新型多能噬菌体。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-07-29 DOI: 10.1007/s00705-025-06370-x

Faizan Ahmad, Vitor Emanuel Lanes Viana, Rafael Reis de Rezende, Samuel Sathler Martuchelle, Anderson Souza Cabral, Ana Luisa Andrade-Oliveira, Isabella Monteiro Carvalho, Sandy de Almada Estanislau, Nohman Rasheed, Poliane Alfenas Zerbini, Monalessa Fábia Pereira, Marcia Giambiagi-deMarval, Ciro César Rossi

{"title":"Discovery of phage CSF, a novel generalist bacteriophage targeting multidrug-resistant and potentially pathogenic Staphylococcus spp. and Mammaliicoccus spp.","authors":"Faizan Ahmad, Vitor Emanuel Lanes Viana, Rafael Reis de Rezende, Samuel Sathler Martuchelle, Anderson Souza Cabral, Ana Luisa Andrade-Oliveira, Isabella Monteiro Carvalho, Sandy de Almada Estanislau, Nohman Rasheed, Poliane Alfenas Zerbini, Monalessa Fábia Pereira, Marcia Giambiagi-deMarval, Ciro César Rossi","doi":"10.1007/s00705-025-06370-x","DOIUrl":"10.1007/s00705-025-06370-x","url":null,"abstract":"<div><p>Antimicrobial resistance is escalating among <i>Staphylococcus</i> species, which are major pathogens affecting both humans and animals. As therapeutic options continue to narrow, phage therapy is re-emerging as a promising strategy to combat resistant strains. In this study, we isolated from pig farm effluents a bacteriophage named CSF, infecting a <i>Staphylococcus xylosus</i> strain. The CSF phage demonstrated a broad host range, lysing <i>mecA</i>-positive, multidrug-resistant strains across nine species of the genera <i>Staphylococcus</i> and <i>Mammaliicoccus</i>. Notably, it also inhibited biofilm formation in strains that it could not infect directly, and this activity persisted at low virus concentrations and in combination with antibiotics. The phage maintained its lytic activity under extreme conditions of temperature, pH, and UV exposure, underscoring its resilience for practical use. Sequencing of the viral genome revealed that it is 140 kb in length and has a genetic composition consistent with a lytic lifestyle. Phylogenetic analysis showed that phage CSF is related to members of the family <i>Herelleviridae</i> that are found globally, suggesting a widespread distribution of related viral lineages. Importantly, the phage genome was found to lack bacterial virulence or antimicrobial resistance genes, suggesting its safety for biotechnological applications. Intergenomic comparisons indicated that CSF should be classified as a member of a new genus, expanding the known diversity of bacteriophages. These findings demonstrate potential of CSF for phage therapy and other biotechnological applications.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coinfections with monkeypox virus and varicella-zoster virus: a systematic review of case reports and case series 猴痘病毒和水痘带状疱疹病毒共感染：病例报告和病例系列的系统回顾。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-07-18 DOI: 10.1007/s00705-025-06359-6

Reem Sayad, Ahmed Saad Elsaeidy, Eslam A. Hawash, Ahmed M. Ali, Alzahraa Mohamed, Ibrahim Khalil

{"title":"Coinfections with monkeypox virus and varicella-zoster virus: a systematic review of case reports and case series","authors":"Reem Sayad, Ahmed Saad Elsaeidy, Eslam A. Hawash, Ahmed M. Ali, Alzahraa Mohamed, Ibrahim Khalil","doi":"10.1007/s00705-025-06359-6","DOIUrl":"10.1007/s00705-025-06359-6","url":null,"abstract":"<div><p>Background: Many studies have shown that, during mpox outbreaks, concomitant infections with monkeypox virus and varicella-zoster virus (VZV) are not uncommon. Infection with either monkeypox virus or VZV may increase the host's susceptibility to contracting a subsequent infection. Here, we summarize the clinical presentation, diagnosis, and management of cases of reported monkeypox virus and VZV coinfection. Methods: We conducted a thorough search of four databases: PubMed, Scopus, Medline, and Web of Science, covering the period up to 10 March 2025, and identified studies reporting coinfections with monkeypox virus and VZV. Data were extracted using a standardized sheet, and case reports and the quality of case series were assessed using the JBI critical appraisal tool. Cross-sectional and cohort quality was assessed using the NIH tool. Results: Our systematic review included 18 studies with 448 cases of coinfection with monkeypox virus and VZV. The majority of cases were reported in African countries, particularly Nigeria and the DRC. Most of the patients were children or young adults, and 41.51% were male. Common predisposing factors included contact with animals, confirmed exposure to infected individuals, and immunocompromised status. Treatment approaches included antivirals (primarily acyclovir or tecovirimat) and supportive care. While most cases were managed successfully, four deaths (0.89%) were reported. Conclusion: This review highlights the complex interplay between monkeypox virus and VZV in coinfections, emphasizing the need for heightened awareness, standardized diagnostic protocols, and tailored management strategies. It is important to consider coinfections when making diagnoses, especially in places where mpox is common or in cases in which the patient is at high risk.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome analysis of a novel mitovirus in the phytopathogenic fungus Penicillium digitatum 植物病原真菌指状青霉中一种新型有丝分裂病毒的全基因组分析。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-07-17 DOI: 10.1007/s00705-025-06364-9

Zhimin Tang, Yunhui He, Ya Yang, Xiaoping Zhang, Rui Zhou, Kang Zhou

引用次数: 0

Growth properties of recombinant equine influenza viruses with different backbones generated by reverse genetics in embryonated chicken eggs 反向遗传不同主干重组马流感病毒在鸡胚蛋中的生长特性。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-07-12 DOI: 10.1007/s00705-025-06368-5

Manabu Nemoto, Nanako Kawanishi, Yoshinori Kambayashi, Hiroshi Bannai, Takashi Yamanaka, Marie Garvey, Ann Cullinane, Seiya Yamayoshi, Yoshihiro Kawaoka, Koji Tsujimura

引用次数: 0

Molecular characterization of a novel mitovirus infecting the phytopathogenic fungus Colletotrichum camelliae 一种感染茶花炭疽菌的新型有丝分裂病毒的分子特征。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-07-11 DOI: 10.1007/s00705-025-06361-y

Hao Zeng, Bin Tian, Liangxuan Qu, Fuyin He, Wei Huang, Shuanglong Wang, Hong Liu, Lihong Cui

引用次数: 0

First detection and tracing of grapevine red blotch virus (GRBV) in Australia using tiled amplicon sequencing 利用平铺扩增子测序在澳大利亚首次检测和追踪葡萄红斑病毒（GRBV）。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-07-10 DOI: 10.1007/s00705-025-06366-7

Tonny Kinene, Andrew S. Taylor, Richard Fennessy, Brenda A. Coutts, David Lovelock, Cuiping Wang, Kamalpreet Kaur, Thao Tran, Brendan Rodoni, Fiona E. Constable, Monica A. Kehoe

{"title":"First detection and tracing of grapevine red blotch virus (GRBV) in Australia using tiled amplicon sequencing","authors":"Tonny Kinene, Andrew S. Taylor, Richard Fennessy, Brenda A. Coutts, David Lovelock, Cuiping Wang, Kamalpreet Kaur, Thao Tran, Brendan Rodoni, Fiona E. Constable, Monica A. Kehoe","doi":"10.1007/s00705-025-06366-7","DOIUrl":"10.1007/s00705-025-06366-7","url":null,"abstract":"<div><p>Grapevine red blotch virus (GRBV; species <i>Grablovirus vitis</i>, genus <i>Grablovirus</i>, family <i>Geminiviridae</i>) poses a significant threat to viticulture worldwide, impacting grapevine health and wine quality. Here, we report the first detection and tracing of GRBV in Australia and provide a summary of a subsequent survey to determine the extent of GRBV distribution in Western Australia. Additionally, the study introduces a tiled amplicon sequencing method, which, when combined with long-read nanopore sequencing, enables rapid GRBV genome sequencing. Our analysis suggests a single introduction of GRBV from North America to Australia through the state of Victoria and subsequently to Western Australia. Finally, this study provides insight into the epidemiology of GRBV, based on strain variation and distribution, which is a crucial step in supporting an emergency biosecurity response and implementing effective control measures to safeguard the sustainability of the wine industry in Australia.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12245990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome sequencing of Pasteurella multocida phage PMP1 elucidates a possible host resistance mechanism and suggests that it belongs to a new species 多杀性巴氏杆菌噬菌体PMP1的基因组测序揭示了一种可能的宿主抗性机制，并表明它属于一个新种。

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-07-10 DOI: 10.1007/s00705-025-06357-8

Gayeong Kim, Beomseok Park, Seunghyeon Ji, Mokhammad Khoiron Ferdiansyah, Yonghwi Kwon, Myeongseong Cha, Manasa Gaddapara, Hwagyun Oh, Kwangpyo Kim

{"title":"Genome sequencing of Pasteurella multocida phage PMP1 elucidates a possible host resistance mechanism and suggests that it belongs to a new species","authors":"Gayeong Kim, Beomseok Park, Seunghyeon Ji, Mokhammad Khoiron Ferdiansyah, Yonghwi Kwon, Myeongseong Cha, Manasa Gaddapara, Hwagyun Oh, Kwangpyo Kim","doi":"10.1007/s00705-025-06357-8","DOIUrl":"10.1007/s00705-025-06357-8","url":null,"abstract":"<div><p><i>Pasteurella multocida</i>, especially capsular serotype A, is a zoonotic pathogen that is found mainly in livestock. While antibiotics are commonly used to treat bacterial infections, bacteriophage therapy has become an important alternative due to the issue of antibiotic resistance. Although four virulent <i>P. multocida</i>-infecting phages have been described, their interactions with their hosts have not been extensively studied. In this study, a novel virulent phage, PMP1, was isolated from a livestock waste treatment facility in Korea. This phage has a short, non-contractile tail with an isometric head and specifically lyses <i>toxA</i>-negative serotype A strains but can adsorb to <i>toxA</i>-positive strains. PMP1 has a genome of 38,049 bp in length with no genes related to lysogeny. Bioinformatic analysis and transmission electron microscopy suggest that a <i>toxA</i>-containing prophage could be involved in the restriction-modification system; however, the correlationof the presence of <i>toxA</i> with phage resistance was not clearly established. The structural proteins were identified for the first time in a <i>P. multocida</i>-infecting phage by LC-MS/MS analysis, and phylogenetic analysis revealed that PMP1 could be a member of new species in the genus <i>Wuhanvirus</i>. These findings suggest that this phage should be investigated further as a potential anti-<i>P. multocida</i> agent.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of the virulent porcine epidemic diarrhea virus strain CHGS20 in China 中国猪流行性腹泻强毒株CHGS20的分离与鉴定

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-07-10 DOI: 10.1007/s00705-025-06334-1

Jingyi Wang, Shuanghui Yin, Sumin Pan, Xiaoqing Zhang, Yupeng Fang, Tian Zhao, Li Li, Jie Zhang, Kazimierz Tarasiuk, Yongsheng Liu, Zygmunt Pejsak, Shunli Yang

{"title":"Isolation and characterization of the virulent porcine epidemic diarrhea virus strain CHGS20 in China","authors":"Jingyi Wang, Shuanghui Yin, Sumin Pan, Xiaoqing Zhang, Yupeng Fang, Tian Zhao, Li Li, Jie Zhang, Kazimierz Tarasiuk, Yongsheng Liu, Zygmunt Pejsak, Shunli Yang","doi":"10.1007/s00705-025-06334-1","DOIUrl":"10.1007/s00705-025-06334-1","url":null,"abstract":"<div><p>Porcine epidemic diarrhea virus (PEDV) is the etiological agent of porcine epidemic diarrhea (PED), which threatens the swine industry worldwide. Although commercial PEDV vaccines are available, the disease continues to plague the swine industry in China. Therefore, isolating PEDV from clinical samples is beneficial for conducting molecular epidemiological investigations, developing effective vaccines and diagnostic methods, and investigating pathogenic mechanisms. In this study, a genotype II PEDV strain, CHGS20, was isolated from a small-intestine sample collected in 2020 from a PEDV-infected piglet and propagated in Vero cells. Virus production in cell culture was confirmed by cytopathology, immunofluorescence assay, and transmission electron microscopy. The infectious virus titers during the first 10 passages ranged from 10<sup>3.3</sup> to 10<sup>7.8</sup> TCID<sub>50</sub> per mL. The inactivated CHGS20 virus was found to induce a potent neutralizing antibody response in immunized mice, with a mean neutralizing antibody titer of 1:136 against the homologous strain and 1:46 against the genotype I strain JX18. The dynamics of the cytopathic effect (CPE) induced by CHGS20 and JX18 were analyzed using an electric cell-substrate impedance sensing system. The results indicated that this CPE assay has the potential to be used to assess the neutralizing activity of antibodies and facilitate the development of antiviral agents. These findings will be helpful for future understanding, control, and prevention of PED.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0