Archives of Virology最新文献

{"title":"Molecular characterization of a new emaravirus infecting Clerodendrum thomsoniae plants","authors":"Vinicius Henrique Bello,&nbsp;Pedro Luis Ramos-González,&nbsp;Gianluca L. Michea Gonzalez,&nbsp;Fernanda de Pádua Del Corona,&nbsp;Jorge Alberto Marques Rezende,&nbsp;Elliot Watanabe Kitajima","doi":"10.1007/s00705-026-06734-x","DOIUrl":"10.1007/s00705-026-06734-x","url":null,"abstract":"<div><p>High-throughput sequencing revealed a new emaravirus named clerodendrum yellow blotch virus (ClYBV), infecting <i>Clerodendrum thomsoniae</i> plants in São Paulo State, Brazil. Four genomic RNA segments (RNA1, RNA3, RNA4, and RNA5) were recovered, whereas RNA2 remained undetected. Phylogenetic analysis and pairwise comparisons based on amino acid sequences showed ClYBV is most closely related to redbud yellow ringspot-associated virus (RYRaV, <i>Emaravirus cercidis</i>). No ClYBV particles were observed by transmission electron microscopy in leaf tissues from infected plants. These results support the identification of ClYBV as a new <i>Emaravirus</i> member, though the undetectable RNA2 suggests the isolate may be defective, lacking this segment and unable to produce virus particles. The binomial species name “<i>Emaravirus clerodendri</i>” is proposed.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13546163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148896506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of chikungunya viruses associated with outbreaks in Kenya from 2017 to 2020","authors":"Derrick Amon,&nbsp;Bonventure Juma,&nbsp;Joseph Muriuki,&nbsp;Caroline Ochieng,&nbsp;Naomi Koech,&nbsp;Gilbert Kikwai,&nbsp;Lydia Mwasi,&nbsp;Melvin Ochieng,&nbsp;Caroline Ngugi,&nbsp;Emily H. Davis,&nbsp;Holly R. Hughes,&nbsp;Aaron C. Brault,&nbsp;Naomi Lucchi,&nbsp;Peninah Munyua,&nbsp;Elizabeth Hunsperger","doi":"10.1007/s00705-026-06729-8","DOIUrl":"10.1007/s00705-026-06729-8","url":null,"abstract":"<div><p>Emerging outbreaks of the arbovirus chikungunya virus affect millions of individuals globally, causing debilitating arthralgia and fever. A study on the burden and etiologies of acute febrile illnesses (AFI) in Kenya reported an increase in CHIKV cases in Mombasa between December 2017 and December 2019. In January and February 2020, another outbreak of CHIKV occurred in Dadaab-Hagadera. Using next-generation sequencing on the Illumina MiSeq platform, we established molecular characteristics and phylogenetic differences of the CHIKVs collected from the two epidemiologically distinct settings. Sequenced viruses belonged to the Indian Ocean Lineage and clustered according to geographic region, with E1:K211E and E2:V264A mutations present in both settings. Additional mutations previously reported during Kenyan CHIKV outbreaks and suggested to influence CHIKV adaptation to <i>Aedes albopictus</i>, including E1:T82I and E1:V84D, were also identified. These molecular markers provide useful information on CHIKV spread and can help strengthen outbreak preparedness and response. Clinical features were described only for laboratory-confirmed CHIKV cases from the AFI surveillance cohort and compared with AFI cases testing negative for CHIKV. Sore muscles, headache, and convulsions were more frequently reported among CHIKV-positive participants, whereas diarrhea was less common. No differences were observed in the frequency of cough, skin rash, conjunctivitis, or vomiting. Given the limited number of confirmed CHIKV cases and the predominance of young children in the cohort, the reported clinical symptom associations should only be interpreted as exploratory and warrant confirmation in larger and more representative populations.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148896537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surveillance of tick-borne encephalitis virus and Tribeč Virus in Ixodes ricinus ticks from Slovakia","authors":"Dana Zubriková,&nbsp;Bronislava Víchová,&nbsp;Katarína Loziaková Peňazziová,&nbsp;Yaroslav Syrota,&nbsp;Lucia Vargová,&nbsp;Veronika Blažeková,&nbsp;Michal Stanko,&nbsp;Klaudia Mária Švirlochová,&nbsp;Soňa Pivka,&nbsp;Petra Schusterová,&nbsp;Tomáš Csank","doi":"10.1007/s00705-026-06735-w","DOIUrl":"10.1007/s00705-026-06735-w","url":null,"abstract":"<div><p>Tick-borne viruses represent a significant and growing public health concern in Europe. This study investigated the minimum infection rates (MIRs) of tick-borne encephalitis virus (TBEV) and Tribeč virus (TRBV) in <i>Ixodes ricinus</i> ticks collected in Slovakia during 2016–2017 and 2023–2024. A total of 3,686 ticks (3,262 nymphs in 359 pools and 424 adults in 99 pools) from 13 sampling sites were screened for viral RNA. The overall MIRs were 0.38% for TBEV and 0.22% for TRBV. All positive detections occurred in nymphal pools. TBEV was detected at 8 of 13 sampling sites, with local prevalence ranging from 0 to 1.32%, whereas TRBV was detected at 6 sites, with local prevalence ranging from 0 to 0.83%. Two nymphal pools were positive for both TBEV and TRBV. Regression analysis indicated a statistically significant positive association between TBEV and TRBV positivity at the pool level. Genetic analysis of the nucleotide sequences obtained for segments 2 and 4 revealed a close evolutionary relationship among the Slovak TRBV strains. These findings suggest possible co-circulation of TBEV and TRBV in I. ricinus populations in selected sites in Slovakia and raise the possibility of co-infections, highlighting the importance of continued surveillance of tick-borne viruses.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of aviadenovirus and dependoparvovirus in an Adélie penguin fecal sample from Cape Royds (Ross Island, Antarctica)","authors":"Melanie Regney,&nbsp;Simona Kraberger,&nbsp;Scott Jennings,&nbsp;Elizabeth L. Porzig,&nbsp;Balázs Harrach,&nbsp;Győző L. Kaján,&nbsp;Judit Pénzes,&nbsp;Katie M. Dugger,&nbsp;Grant Ballard,&nbsp;David G. Ainley,&nbsp;Arvind Varsani","doi":"10.1007/s00705-026-06726-x","DOIUrl":"10.1007/s00705-026-06726-x","url":null,"abstract":"<div><p>Adenoviruses (<i>Adenoviridae</i>) and their associated replication-deficient dependoparvoviruses (<i>Parvoviridae</i>) are recognized as significant components of the avian virome. While well-studied in domestic birds, these viruses remain underexplored in wild Antarctic birds. Information is limited on viruses circulating in penguins, particularly outside of the Antarctic Peninsula Region. Here we leverage a fecal sample collected for diet analyses to identify an aviadenovirus (37,731 nt) and two distinct dependoparvoviruses. The Adélie penguin (<i>Pygoscelis adeliae</i>) feces-derived adenovirus exhibits a genome organization consistent with members of the genus <i>Aviadenovirus</i> but shares only ~64% amino acid identity in the DNA polymerase protein with its closest known relative and thus represents a new species of aviadenovirus identified in penguins. Additionally, we identified two new dependoparvoviruses, one in length of 4,869 nt and a second of 4,162 nt. These two dependoparvoviruses are diverse, sharing &lt;61% Rep amino acid identity with those of other dependoparvoviruses. Penguin dependoparvovirus 1 represents a new species. The co-detection of these viruses in a single seasonal sample could suggest a potential helper-satellite relationship in Adélie penguins. This study provides the first evidence of aviadenoviruses in Adélie penguin feces. These findings highlight the importance of surveillance to understand pathogen dynamics in isolated Antarctic populations.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel avian calicivirus genome with type IV internal ribosomal entry site (IRES) in black-headed gull (Chroicocephalus ridibundus) in Hungary","authors":"Gábor Reuter,&nbsp;Benigna Balázs,&nbsp;Róbert Mátics,&nbsp;Ákos Boros,&nbsp;Péter Pankovics","doi":"10.1007/s00705-026-06733-y","DOIUrl":"10.1007/s00705-026-06733-y","url":null,"abstract":"<div><p>In this study, a taxonomically novel avian calicivirus detected and characterized by next generation sequencing, RT-PCR and Sanger sequencing methods in faecal specimen collected from black-headed gull (<i>Chroicocephalus ridibundus</i>) in Hungary. The complete genome length of the calicivirus strain gull/HA15097/HUN/2018 (PZ810127) is remarkably long, 8,845 nucleotides, which had type IV internal ribosomal entry site (IRES) at the 5’, and a stem-loop-II-like (s2m) sequence motif at the 3’ untranslated regions. The VP1 capsid protein had less than 26% aa identity to the members of the known calicivirus genera. Caliciviruses appear to be widespread not only in mammals including humans but also in various bird species.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-026-06733-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148871708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Biological risk assessment related to intracerebral injection of TMEV-DA","authors":"Iskra Djabirska,&nbsp;Laetitia Delaval,&nbsp;Audrey Tromme,&nbsp;Joël Blomet,&nbsp;Daniel Desmecht,&nbsp;Anne-Sophie Van Laere","doi":"10.1007/s00705-026-06712-3","DOIUrl":"10.1007/s00705-026-06712-3","url":null,"abstract":"","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148871705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of a novel partitivirus harboring an additional third dsRNA segment from Trichoderma harzianum","authors":"Jeong Eun Kim,&nbsp;Jeesun Chun,&nbsp;Yo-Han Ko,&nbsp;Dae-Hyuk Kim","doi":"10.1007/s00705-026-06731-0","DOIUrl":"10.1007/s00705-026-06731-0","url":null,"abstract":"<div><p>We report the complete genome sequence of a novel partitivirus identified from <i>Trichoderma harzianum</i> NFCF092 strain, designated Trichoderma harzianum partitivirus 4 (ThPV4). Unlike canonical members of the family <i>Partitiviridae</i>, which possess a bipartite genome consisting of two double-stranded RNA (dsRNA) segments encoding an RNA-dependent RNA polymerase (RdRP) and a capsid protein (CP), ThPV4 harbors a third dsRNA segment encoding a protein of unknown function. The complete genome consists of dsRNA1 (1,950 bp; encoding the RdRP), dsRNA2 (1,772 bp; encoding the CP), and dsRNA3 (1,629 bp; encoding a protein with unknown function). Sequence analysis shows that each segment possesses a single open reading frame (ORF). The deduced amino acid sequence of the RdRP shows the highest similarity (90.5% identity) to that of Trichoderma gamsii alphapartitivirus 1. Phylogenetic analyses based on the RdRP indicate that ThPV4 clusters within the genus <i>Alphapartitivirus</i> of the family <i>Partitiviridae</i>. To our knowledge. ThPV4 is the first member of the genus <i>Alphapartitivirus</i> identified from <i>T. harzianum</i> to possess an additional, conserved third dsRNA segment.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148873015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analysis of stem-loop structures within the SARS-CoV-2 5′ untranslated region using a plasmid-based reporter system","authors":"Sylvia Yahumin,&nbsp;Ping Chin Lee,&nbsp;Caroline Sunggip,&nbsp;Kenneth Francis Rodrigues","doi":"10.1007/s00705-026-06732-z","DOIUrl":"10.1007/s00705-026-06732-z","url":null,"abstract":"<div><p>The 5′ untranslated region (5′UTR) of <i>severe acute respiratory syndrome coronavirus 2</i> (SARS-CoV-2) contains highly conserved stem-loop structures that regulate viral gene expression. This study investigated the functional contributions of selected 5′UTR stem-loop elements to reporter gene expression using a plasmid-based mammalian expression system. Five constructs were tested using a non-integrating plasmid: the wild-type (WT) 5′UTR fused to GFP under the CMV promoter, and four deletion variants (ΔB, ΔC, ΔD, and ΔE) corresponding to deletions of stem-loop 4 (SL4), SL4.5, SL5, and SL5a, respectively. Following transfection into HEK293 cells, GFP fluorescence was quantified using a fluorescence microplate reader, and relative GFP transcript abundance was assessed by RT-qPCR. Deletion of SL4 (ΔB) resulted in marked reduction in both fluorescence and relative transcript abundance compared to WT construct, indicating substantially reduced reporter gene expression. In contrast, deletion of SL4.5, SL5, or SL5a did not produce the pronounced reduction observed for ΔB, although descriptive RT-qPCR analysis indicated differences in relative transcript abundance among these variants. Statistical analysis of fluorescence data demonstrated significant differences among constructs (one-way ANOVA, <i>p</i> &lt; 0.05). Because the reporter assay was based on plasmid expression, the observed differences likely reflect combined contributions from transcription, transcript abundance, RNA stability, and translation rather than translation alone. These findings demonstrate that the SL4 region contributes substantially to reporter gene expression in this experimental system, whereas the remaining stem-loop regions examined exert comparatively modest effects. This study provides additional insight into the functional organization of the SARS-CoV-2 5′UTR and establishes a framework for future investigations aimed at distinguished the transcriptional, post-transcriptional, and translational contributions of individual RNA structural elements.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a novel papillomavirus in the migratory black-headed gull (Chroicocephalus ridibundus) from southwestern China","authors":"Xiaoliang Hu,&nbsp;Xixi Chen,&nbsp;Ye Tang,&nbsp;Min Zeng,&nbsp;Bangyuan Wu,&nbsp;Dongwei Wang,&nbsp;Zhige Tian","doi":"10.1007/s00705-026-06728-9","DOIUrl":"10.1007/s00705-026-06728-9","url":null,"abstract":"<div><p>In the present study, a papillomavirus, designated CrPV1, was identified for the first time in fecal samples collected from black-headed gulls (<i>Chroicocephalus ridibundus</i>) in southwestern China using virus-specific polymerase chain reaction analysis. The complete CrPV1 genome was 7 794 bp in length. Phylogenetic analysis based on the complete genome showed that CrPV1 clustered closely with <i>Columba livia</i> papillomavirus 1 and was clearly distinct from previously reported papillomaviruses, including <i>Psittacus erithacus papillomavirus</i> (PePV1), <i>Francolinus leucoscepus papillomavirus 1</i> (FlPV1), and <i>Fringilla coelebs papillomavirus</i> (FcPV1). These findings expand current knowledge of papillomavirus diversity in gulls and provide new insight into the evolutionary origin of papillomaviruses in this host group.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of a Nervous Necrosis Virus (NNV) isolated from tilapia (Oreochromis niloticus) in China","authors":"Dapeng Yu,&nbsp;Tingting Weng,&nbsp;Hongli Xia,&nbsp;Liqun Xia,&nbsp;Yishan Lu,&nbsp;Zhiwen Wang,&nbsp;Jia Cai","doi":"10.1007/s00705-026-06716-z","DOIUrl":"10.1007/s00705-026-06716-z","url":null,"abstract":"<div><p>Nervous necrosis virus (NNV) is a viral pathogen that infected variety of cultured and farmed fish around the world. In this study, we isolated a novel NNV from diseased cultured tilapia (<i>Oreochromis niloticus</i>). The typical vacuolates were discovered in the brain of diseased tilapia through histological analysis. The virus was identified as NNV with RT-PCR tested of brain tissues and named as ONNV-ZS. In addition, the virus was purified by incubating GS cells with tissue suspension of brain from diseased <i>O. niloticus.</i> The obvious CPE (cytopathic effect) was observed in infected cells and the virus was confirmed by RT-PCR. Electron microscopy of the infected cells revealed the virus particles with non-enveloped spherical shape. The pathogenicity of ONNV-ZS was explored in challenge experiments using <i>O. niloticus</i> juveniles. The cumulative mortalities reached 100% at day 10 post-infection. Together, this is the first report for isolation and identification of a NNV isolate from <i>O. niloticus</i> in China.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148838168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}