{"title":"一种新型小麦感染鲁特病毒的全核苷酸序列和基因组组织。","authors":"Zhiguo Zhou, Xue Feng, Linzhu Liang, Wenya Liu, Zixuan Zhou, Xinyuan Zhang, Zhongtian Xu, Peipei Zhang","doi":"10.1007/s00705-025-06336-z","DOIUrl":null,"url":null,"abstract":"<p><p>To determine the prevalence of wheat-infecting viruses, a viromic survey of wheat plants exhibiting typical plant virus symptoms collected in Hebei province, China, was conducted using rRNA-depleted RNA-seq. A novel luteovirus was discovered and tentatively named \"wheat luteovirus 1\" (WLV1). RT-PCR and RACE-PCR were performed to determine the complete genome sequence of WLV1, which was found to consist of 5,682 nucleotides (nt), with six AUG-initiated ORFs and one non-AUG-initiated ORF, and to have typical characteristics of members of the genus Luteovirus. Sequence comparisons and phylogenetic analysis indicated that WLV1 was most closely related to several barley yellow dwarf viruses (BYDVs) belonging to the genus Luteovirus, but the level of sequence identity was below the species demarcation threshold (90%). This finding therefore adds a new luteovirus to the list of viruses associated with wheat yellow dwarf disease.</p>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"145"},"PeriodicalIF":2.5000,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The complete nucleotide sequence and genome organization of a novel wheat-infecting luteovirus.\",\"authors\":\"Zhiguo Zhou, Xue Feng, Linzhu Liang, Wenya Liu, Zixuan Zhou, Xinyuan Zhang, Zhongtian Xu, Peipei Zhang\",\"doi\":\"10.1007/s00705-025-06336-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>To determine the prevalence of wheat-infecting viruses, a viromic survey of wheat plants exhibiting typical plant virus symptoms collected in Hebei province, China, was conducted using rRNA-depleted RNA-seq. A novel luteovirus was discovered and tentatively named \\\"wheat luteovirus 1\\\" (WLV1). RT-PCR and RACE-PCR were performed to determine the complete genome sequence of WLV1, which was found to consist of 5,682 nucleotides (nt), with six AUG-initiated ORFs and one non-AUG-initiated ORF, and to have typical characteristics of members of the genus Luteovirus. Sequence comparisons and phylogenetic analysis indicated that WLV1 was most closely related to several barley yellow dwarf viruses (BYDVs) belonging to the genus Luteovirus, but the level of sequence identity was below the species demarcation threshold (90%). This finding therefore adds a new luteovirus to the list of viruses associated with wheat yellow dwarf disease.</p>\",\"PeriodicalId\":8359,\"journal\":{\"name\":\"Archives of Virology\",\"volume\":\"170 7\",\"pages\":\"145\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-06-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Virology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s00705-025-06336-z\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VIROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Virology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00705-025-06336-z","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
The complete nucleotide sequence and genome organization of a novel wheat-infecting luteovirus.
To determine the prevalence of wheat-infecting viruses, a viromic survey of wheat plants exhibiting typical plant virus symptoms collected in Hebei province, China, was conducted using rRNA-depleted RNA-seq. A novel luteovirus was discovered and tentatively named "wheat luteovirus 1" (WLV1). RT-PCR and RACE-PCR were performed to determine the complete genome sequence of WLV1, which was found to consist of 5,682 nucleotides (nt), with six AUG-initiated ORFs and one non-AUG-initiated ORF, and to have typical characteristics of members of the genus Luteovirus. Sequence comparisons and phylogenetic analysis indicated that WLV1 was most closely related to several barley yellow dwarf viruses (BYDVs) belonging to the genus Luteovirus, but the level of sequence identity was below the species demarcation threshold (90%). This finding therefore adds a new luteovirus to the list of viruses associated with wheat yellow dwarf disease.
期刊介绍:
Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.