一种新型小麦感染鲁特病毒的全核苷酸序列和基因组组织。

IF 2.5 4区 医学 Q3 VIROLOGY
Zhiguo Zhou, Xue Feng, Linzhu Liang, Wenya Liu, Zixuan Zhou, Xinyuan Zhang, Zhongtian Xu, Peipei Zhang
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引用次数: 0

摘要

为了确定小麦侵染病毒的流行程度,采用rrna -贫RNA-seq技术对在中国河北省收集的表现典型植物病毒症状的小麦植株进行了病毒组学调查。发现了一种新的黄体病毒,暂命名为小麦黄体病毒1号(WLV1)。RT-PCR和RACE-PCR检测了WLV1的全基因组序列,发现该病毒包含5682个核苷酸(nt),具有6个由aug启动的ORF和1个非aug启动的ORF,具有lutevirus属成员的典型特征。序列比较和系统发育分析表明,WLV1与若干属lutevirus属的大麦黄矮病毒(BYDVs)亲缘关系最为密切,但序列同源性低于种界阈值(90%)。因此,这一发现在与小麦黄矮病相关的病毒列表中增加了一种新的鲁特病毒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The complete nucleotide sequence and genome organization of a novel wheat-infecting luteovirus.

To determine the prevalence of wheat-infecting viruses, a viromic survey of wheat plants exhibiting typical plant virus symptoms collected in Hebei province, China, was conducted using rRNA-depleted RNA-seq. A novel luteovirus was discovered and tentatively named "wheat luteovirus 1" (WLV1). RT-PCR and RACE-PCR were performed to determine the complete genome sequence of WLV1, which was found to consist of 5,682 nucleotides (nt), with six AUG-initiated ORFs and one non-AUG-initiated ORF, and to have typical characteristics of members of the genus Luteovirus. Sequence comparisons and phylogenetic analysis indicated that WLV1 was most closely related to several barley yellow dwarf viruses (BYDVs) belonging to the genus Luteovirus, but the level of sequence identity was below the species demarcation threshold (90%). This finding therefore adds a new luteovirus to the list of viruses associated with wheat yellow dwarf disease.

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来源期刊
Archives of Virology
Archives of Virology 医学-病毒学
CiteScore
5.10
自引率
7.40%
发文量
324
审稿时长
4.5 months
期刊介绍: Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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