表达鸭天布苏病毒包膜蛋白的重组鸭肠炎病毒的构建与评价。

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-06-10 DOI:10.1007/s00705-025-06342-1

Lihua Wang, Xiaoyue Yang, Bing Zhang, Siyuan Hao, Yafen Song, Dan Li, Min Zhang, Qianyi Zhang, Yongda Zhao, Chenghuai Yang

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引用次数: 0

摘要

鸭坦布苏病毒（DTMUV）对中国水禽养殖业构成重大威胁。本研究利用同源重组和Cre-Lox系统构建了表达DTMUV E蛋白的重组鸭肠炎病毒（DEV），命名为rDEV-ERT。DTMUV E基因被整合到DEV基因组的UL2区域。在疫苗接种后14天，60 TCID50的rDEV-ERT对致命的DEV攻击提供了完全的保护。28 dpv时，rDEV-ERT诱导的DTMUV抗体阳性率为50%，对DTMUV毒株的保护率为62.5%。这些发现支持开发一种DEV-DTMUV重组活载体疫苗。

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Construction and evaluation of a recombinant duck enteritis virus expressing the envelope protein of duck Tembusu virus in ducks

Duck Tembusu virus (DTMUV) poses a significant threat to the waterfowl breeding industry in China. Here, we constructed a recombinant duck enteritis virus (DEV) expressing the entire E protein of DTMUV, named rDEV-ERT, using homologous recombination and the Cre-Lox system. The DTMUV E gene was integrated into the UL2 region of the DEV genome. At 14 days post-vaccination (dpv), 60 TCID₅₀ of rDEV-ERT provided complete protection against lethal DEV challenge. At 28 dpv, the positive rate of DTMUV antibodies induced by rDEV-ERT was 50%, and the protection rate against a virulent DTMUV strain was 62.5%. These findings support the development of a DEV-DTMUV recombinant live vector vaccine.

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.