犬兔病毒:一种新型犬肠道病原体的分离与分子鉴定。

IF 2.5 4区 医学 Q3 VIROLOGY
Gulizar Acar, Hayrunnisa Bostan Yoru, Erdal Polat, Ahsen Nisa Aslan, Hakan Aydin, Mehmet Ozkan Timurkan
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引用次数: 0

摘要

犬bufavv (CBuV)最初是在有呼吸道症状的狗身上发现的,后来在粪便样本中发现,这表明它可能是一种肠道病原体。几项研究表明,CBuV可以存在于健康和腹泻的狗身上,这就提出了CBuV是否是主要肠道病原体的问题。在这项研究中,我们从狗的拭子样本中鉴定、分离和表征了CBuV。采用PCR方法对119份腹泻犬样本进行了分析,目标是CBuV的一部分VP2基因。5只犬检出CBuV(4.2%)。将CBuV阳性样本接种于MDCK细胞培养物上,观察CPE,检测CBuV以及其他常见的肠道病原体(犬瘟热病毒、犬细小病毒2型和犬冠状病毒),但CBuV是唯一检测到的病毒。系统发育分析表明,本研究分离的CBuV株与中国和印度的菌株亲缘关系密切。本研究中CBuV的检测表明它可能在犬腹泻中起着独特的作用。其遗传特征、流行病学及生物学意义有待进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and molecular identification of canine bufavirus: a novel enteric pathogen of dogs.

Canine bufavirus (CBuV) was initially detected in dogs with respiratory symptoms and later identified in fecal samples, suggesting that it could be an enteric pathogen. Several studies have shown that CBuV can be present in both healthy and diarrheic dogs, raising questions about whether CBuV is a primary enteric pathogen. In this study, we identified, isolated, and characterized CBuV from swab samples from dogs. A total of 119 samples from diarrheic dogs were analyzed by PCR, targeting a portion of the VP2 gene of CBuV. CBuV was detected in five dogs (4.2%). For virus isolation, CBuV-positive samples were inoculated onto an MDCK cell culture, and CPE was observed for one sample, which was tested for CBuV as well as other frequently detected enteric pathogens (canine distemper virus, canine parvovirus type 2, and canine coronavirus), but CBuV was the only virus detected. Phylogenetic analysis indicated that the CBuV isolates from this study are closely related to strains from China and India. The detection of CBuV in this study suggests that it might play a distinct role in canine diarrhea. Further studies are needed to investigate its genetic characteristics, epidemiology, and biological significance.

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来源期刊
Archives of Virology
Archives of Virology 医学-病毒学
CiteScore
5.10
自引率
7.40%
发文量
324
审稿时长
4.5 months
期刊介绍: Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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