{"title":"中国喀斯特溶洞新型窄养单胞菌噬菌体vB_Srh_LBjhp91a的基因组分析","authors":"Lou Ren, Zheng Fang, Shixia Li, Qingshan Wu, Lan Xiang, Chuangen Lin, Qiuping Liu, Leitao Tan, Qingbei Weng","doi":"10.1007/s00705-025-06329-y","DOIUrl":null,"url":null,"abstract":"<p><p>Stenotrophomonas phage vB_Srh_LBjhp91a was isolated from a karst cave, and its genome was sequenced. This phage has a 63,966-bp circular dsDNA genome (60.7% G + C content) containing 83 predicted open reading frames and two tRNAs. A BLASTn search revealed 80.95% nucleotide sequence identity to Stenotrophomonas phage vB_Sm_QDWS359 (genus Xooduovirus, family Mesyanzhinovviridae). Phylogenetic analysis based on the DNA polymerase and terminase large subunit genes grouped vB_Srh_LBjhp91a with members of the genera Bosavirus, Elanorvirus, and Xooduovirus. Proteomic phylogenetic analysis further clustered it with phage vB_Sm_QDWS359. Sequence comparisons revealed 71.7% average nucleotide identity between phage vB_Srh_LBjhp91a and phage vB_Sm_QDWS359. These findings indicate that phage vB_Srh_LBjhp91a should be considered a member of a new species within the genus Xooduovirus.</p>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 7","pages":"142"},"PeriodicalIF":2.5000,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genomic analysis of the novel Stenotrophomonas phage vB_Srh_LBjhp91a, isolated from a Karst cave in China.\",\"authors\":\"Lou Ren, Zheng Fang, Shixia Li, Qingshan Wu, Lan Xiang, Chuangen Lin, Qiuping Liu, Leitao Tan, Qingbei Weng\",\"doi\":\"10.1007/s00705-025-06329-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Stenotrophomonas phage vB_Srh_LBjhp91a was isolated from a karst cave, and its genome was sequenced. This phage has a 63,966-bp circular dsDNA genome (60.7% G + C content) containing 83 predicted open reading frames and two tRNAs. A BLASTn search revealed 80.95% nucleotide sequence identity to Stenotrophomonas phage vB_Sm_QDWS359 (genus Xooduovirus, family Mesyanzhinovviridae). Phylogenetic analysis based on the DNA polymerase and terminase large subunit genes grouped vB_Srh_LBjhp91a with members of the genera Bosavirus, Elanorvirus, and Xooduovirus. Proteomic phylogenetic analysis further clustered it with phage vB_Sm_QDWS359. Sequence comparisons revealed 71.7% average nucleotide identity between phage vB_Srh_LBjhp91a and phage vB_Sm_QDWS359. These findings indicate that phage vB_Srh_LBjhp91a should be considered a member of a new species within the genus Xooduovirus.</p>\",\"PeriodicalId\":8359,\"journal\":{\"name\":\"Archives of Virology\",\"volume\":\"170 7\",\"pages\":\"142\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-05-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Virology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s00705-025-06329-y\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VIROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Virology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00705-025-06329-y","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
Genomic analysis of the novel Stenotrophomonas phage vB_Srh_LBjhp91a, isolated from a Karst cave in China.
Stenotrophomonas phage vB_Srh_LBjhp91a was isolated from a karst cave, and its genome was sequenced. This phage has a 63,966-bp circular dsDNA genome (60.7% G + C content) containing 83 predicted open reading frames and two tRNAs. A BLASTn search revealed 80.95% nucleotide sequence identity to Stenotrophomonas phage vB_Sm_QDWS359 (genus Xooduovirus, family Mesyanzhinovviridae). Phylogenetic analysis based on the DNA polymerase and terminase large subunit genes grouped vB_Srh_LBjhp91a with members of the genera Bosavirus, Elanorvirus, and Xooduovirus. Proteomic phylogenetic analysis further clustered it with phage vB_Sm_QDWS359. Sequence comparisons revealed 71.7% average nucleotide identity between phage vB_Srh_LBjhp91a and phage vB_Sm_QDWS359. These findings indicate that phage vB_Srh_LBjhp91a should be considered a member of a new species within the genus Xooduovirus.
期刊介绍：
Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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