Coordinated role of RIG-I, MDA5, and TLR7 in the innate immune response to duck hepatitis A virus 3

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-09-24 DOI:10.1007/s00705-025-06384-5

Duo Peng, Xiaoyang Hu, Jingjing Huang, Qiong Li, Yinuo Song, Dabing Zhang

引用次数: 0

Abstract

The roles of RIG-I, MDA5, and TLR7 in duck hepatitis A virus genotype 3 (DHAV-3) infection were investigated using duck embryo fibroblasts (DEFs). DHAV-3 infection induced significant upregulation of RIG-I, MDA5, and TLR7 and high IFN-β, IL-6, and OASL responses. Overexpression and knockdown of RIG-I, MDA5, and TLR7 exerted significant effects on DHAV-3-induced IFN-β, IL-6, and OASL expression and DHAV-3 replication. Overexpression and inhibition of TLR7 altered DHAV-3-induced RIG-I and MDA5 expression. Together, these findings suggest that TLR7 and RIG-I/MDA5 play a coordinated role in detection and initiation of innate immunity against DHAV-3 infection.

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rig - 1、MDA5和TLR7在鸭甲型肝炎病毒先天免疫应答中的协同作用

采用鸭胚成纤维细胞（DEFs）研究了rig - 1、MDA5和TLR7在鸭甲型肝炎病毒基因3型（DHAV-3）感染中的作用。DHAV-3感染诱导rig - 1、MDA5和TLR7显著上调，IFN-β、IL-6和OASL反应升高。rig - 1、MDA5和TLR7的过表达和下调对DHAV-3诱导的IFN-β、IL-6和OASL的表达和DHAV-3的复制有显著影响。TLR7的过表达和抑制改变了dhav -3诱导的rig - 1和MDA5的表达。总之，这些发现表明TLR7和rig - 1 /MDA5在检测和启动针对DHAV-3感染的先天免疫中发挥协调作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.