Archives of Virology最新文献_第9页

Complete genome sequence of a novel mitovirus isolated from the phytopathogenic fungus Alternaria alternata causing apple leaf blotch 从导致苹果叶斑病的植物病原真菌 Alternaria alternata 中分离出的新型丝裂病毒的完整基因组序列。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-08-06 DOI: 10.1007/s00705-024-06106-3

Jichun Jia, Hanyang Liang, Lihong Cheng, Jinsheng Xia, Xu Chen, Baojun Zhang, Fan Mu

引用次数: 0

Baculovirus expression and purification of virion core and envelope proteins of goatpox virus to evaluate their diagnostic potential 杆状病毒表达和纯化山羊痘病毒的病毒核心蛋白和包膜蛋白，以评估其诊断潜力。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-08-03 DOI: 10.1007/s00705-024-06079-3

Anand Kushwaha, Amit Kumar, S. Chandrasekhar, G. Poulinlu, Karam Chand, D. Muthuchelvan, G. Venkatesan

{"title":"Baculovirus expression and purification of virion core and envelope proteins of goatpox virus to evaluate their diagnostic potential","authors":"Anand Kushwaha, Amit Kumar, S. Chandrasekhar, G. Poulinlu, Karam Chand, D. Muthuchelvan, G. Venkatesan","doi":"10.1007/s00705-024-06079-3","DOIUrl":"10.1007/s00705-024-06079-3","url":null,"abstract":"<div><p>Goatpox and sheeppox are highly contagious and economically important viral diseases of small ruminants. Due to the risk they pose to animal health, livestock production, and international trade, capripoxviruses are a considerable threat to the livestock economy. In this study, we expressed two core proteins (A4L and A12L) and one extracellular enveloped virion protein (A33R) of goatpox virus in a baculovirus expression vector system and evaluated their use as diagnostic antigens in ELISA. Full-length A4L, A12L, and A33R genes of the GTPV Uttarkashi strain were amplified, cloned into the pFastBac HT A donor vector, and introduced into DH10Bac cells containing a baculovirus shuttle vector plasmid to generate recombinant bacmids. The recombinant baculoviruses were produced in Sf-21 cells by transfection, and proteins were expressed in TN5 insect cells. The recombinant proteins were analysed by SDS-PAGE and confirmed by western blot, with expected sizes of ~30 kDa, ~31 kDa, and ~32 kDa for A4L, A12L, and A33R, respectively. The recombinant proteins were purified, and the immunoreactivity of the purified proteins was confirmed by western blot using anti-GTPV serum. The antigenic specificity of the expressed proteins as diagnostic antigens was evaluated by testing their reactivity with infected, vaccinated, and negative GTPV/SPPV serum in indirect ELISA, and the A33R-based indirect ELISA was optimized. The diagnostic sensitivity and specificity of the A33R-based indirect ELISA were found to be of 89% and 94% for goats and 98% and 91%, for sheep, respectively. No cross-reactivity was observed with other related viruses. The recombinant-A33R-based indirect ELISA developed in the present study shows that it has potential for the detection of antibodies in GTPV and SPPV infected/vaccinated animals.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infection with bovine leukemia virus belonging to group A or B-1 contributes more strongly to the development of enzootic bovine leukosis in young cattle than the presence of bovine lymphocyte antigen-DRB3 susceptibility alleles 与存在牛淋巴细胞抗原-DRB3易感等位基因相比，感染属于 A 组或 B-1 组的牛白血病病毒更容易导致幼牛发生牛白血病。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-08-01 DOI: 10.1007/s00705-024-06102-7

Yuki Fujii, Masaki Maezawa, Masataka Akagami, Junko Kawakami, Yuri Fujimoto, Hisashi Inokuma

引用次数: 0

In vitro synergistic antiviral activity of repurposed drugs against enterovirus 71 针对肠道病毒 71 的再利用药物的体外协同抗病毒活性。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-30 DOI: 10.1007/s00705-024-06097-1

Kunlakanya Jitobaom, Chompunuch Boonarkart, Songkran Thongon, Thanyaporn Sirihongthong, Arpakorn Sornwong, Prasert Auewarakul, Ornpreya Suptawiwat

{"title":"In vitro synergistic antiviral activity of repurposed drugs against enterovirus 71","authors":"Kunlakanya Jitobaom, Chompunuch Boonarkart, Songkran Thongon, Thanyaporn Sirihongthong, Arpakorn Sornwong, Prasert Auewarakul, Ornpreya Suptawiwat","doi":"10.1007/s00705-024-06097-1","DOIUrl":"10.1007/s00705-024-06097-1","url":null,"abstract":"<div><p>Enteroviruses cause viral diseases that are harmful to children. Hand, foot, and mouth disease (HFMD) with neurological complications is mainly caused by enterovirus 71 (EV71). Despite its clinical importance, there is no effective antiviral drug against EV71. However, several repurposed drugs have been shown to have antiviral activity against related viruses. Treatments with single drugs and two-drug combinations were performed <i>in vitro</i> to assess anti-EV71 activity. Three repurposed drug candidates with broad-spectrum antiviral activity were found to demonstrate potent anti-EV71 activity: prochlorperazine, niclosamide, and itraconazole. To improve antiviral activity, combinations of two drugs were tested. Niclosamide and itraconazole showed synergistic antiviral activity in Vero cells, whereas combinations of niclosamide-prochlorperazine and itraconazole-prochlorperazine showed only additive effects. Furthermore, the combination of itraconazole and prochlorperazine showed an additive effect in neuroblastoma cells. Itraconazole and prochlorperazine exert their antiviral activities by inhibiting Akt phosphorylation. Repurposing of drugs can provide a treatment solution for HFMD, and our data suggest that combining these drugs can enhance that efficacy.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141791776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the diagnostic sensitivity and specificity of two pen-side tests for detecting African swine fever virus in experimentally infected pigs 评估用于检测实验感染猪体内非洲猪瘟病毒的两种栏边检测方法的诊断灵敏度和特异性。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-30 DOI: 10.1007/s00705-024-06098-0

Hanh D. Vu, Hung Q. Luong, Huong T.L. Lai, Hoa T. Nguyen, Trang H. Pham, Lam Q. Truong, Giap V. Nguyen, Hiep L.X. Vu

{"title":"Evaluation of the diagnostic sensitivity and specificity of two pen-side tests for detecting African swine fever virus in experimentally infected pigs","authors":"Hanh D. Vu, Hung Q. Luong, Huong T.L. Lai, Hoa T. Nguyen, Trang H. Pham, Lam Q. Truong, Giap V. Nguyen, Hiep L.X. Vu","doi":"10.1007/s00705-024-06098-0","DOIUrl":"10.1007/s00705-024-06098-0","url":null,"abstract":"<div><p>African swine fever virus (ASFV) has spread through many countries and regions worldwide, causing significant losses. Timely detection of ASFV-infected pigs is crucial for disease control. In this study, we assessed the performance of two pen-side tests: a portable real-time PCR (qPCR) test for detecting viral genomic DNA and a lateral flow immunoassay (LFIA) for detecting viral antigens. To determine the time from infection to the earliest detection, 10 ASFV-seronegative pigs were inoculated intramuscularly with 10<sup>4.0</sup> hemadsorption dose 50 of a highly virulent ASFV strain. Whole blood and oral swab samples were alternately collected from each group of five pigs daily until all succumbed to the infection. Samples were promptly subjected to the two pen-side tests upon collection, and a subset was transported to a veterinary diagnostic laboratory for analysis using a reference qPCR assay. Viral genomic DNA was consistently detected by the reference qPCR assay in all blood samples from 2 days postinfection (dpi), preceding the onset of clinical signs, and in oral swabs from 4 dpi onwards. The portable qPCR test demonstrated comparable performance to the reference qPCR assay for both whole blood and oral swab samples. The LFIA exhibited 100% specificity when testing with whole blood samples but showed reduced sensitivity, particularly for blood samples collected early or late after infection. The antigen test did not perform well with oral swabs.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11289199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome sequences of three Prunus-infecting nepoviruses: apricot latent ringspot virus, myrobalan latent ringspot virus, and a novel virus from smooth stone peach (Prunus mira Koehne) 三种梅花感染性肾病毒的完整基因组序列：杏潜伏环斑病毒、桃花潜伏环斑病毒和一种来自光滑石桃（Prunus mira Koehne）的新型病毒。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-17 DOI: 10.1007/s00705-024-06091-7

M. Khalili, T. Candresse, C. Faure, Y. Brans, A. Marais

引用次数: 0

GB and gH/gL fusion machinery: a promising target for vaccines to prevent Epstein-Barr virus infection GB 和 gH/gL 融合机制：预防 Epstein-Barr 病毒感染的疫苗的一个有希望的目标。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-17 DOI: 10.1007/s00705-024-06095-3

Changqing Liu, Shan Li, Muchuan Qiao, Chenlu Zeng, Xiaomin Liu, Yunlian Tang

引用次数: 0

Identification of a novel member of the genus Laulavirus (family Phenuiviridae) from the entomopathogenic ascomycete fungus Cordyceps javanica 从昆虫病原真菌冬虫夏草中鉴定出 Laulavirus 属（Phenuiviridae 科）的一个新成员。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-12 DOI: 10.1007/s00705-024-06069-5

Xinran Cao, Bo Liu, Ziqi Wang, Tianxing Pang, Liying Sun, Hideki Kondo, Junmin Li, Ida Bagus Andika, Shengqi Chi

{"title":"Identification of a novel member of the genus Laulavirus (family Phenuiviridae) from the entomopathogenic ascomycete fungus Cordyceps javanica","authors":"Xinran Cao, Bo Liu, Ziqi Wang, Tianxing Pang, Liying Sun, Hideki Kondo, Junmin Li, Ida Bagus Andika, Shengqi Chi","doi":"10.1007/s00705-024-06069-5","DOIUrl":"10.1007/s00705-024-06069-5","url":null,"abstract":"<div><p>The virus family <i>Phenuiviridae</i> (order <i>Hareavirales</i>, comprising segmented negative-sense single stranded RNA viruses) has highly diverse members that are known to infect animals, plants, protozoans, and fungi. In this study, we identified a novel phenuivirus infecting a strain of the entomopathogenic fungus <i>Cordyceps javanica</i> isolated from a small brown plant hopper (<i>Laodelphax striatellus</i>), and this virus was tentatively named \"Cordyceps javanica negative-strand RNA virus 1\" (CjNRSV1). The CjNRSV1 genome consists of three negative-sense single stranded RNA segments (RNA1–3) with lengths of 7252, 2401, and 1117 nt, respectively. The 3′- and 5′-terminal regions of the RNA1, 2, and 3 segments have identical sequences, and the termini of the RNA segments are complementary to each other, reflecting a common characteristic of viruses in the order <i>Hareavirales</i>. RNA1 encodes a large protein (∼274 kDa) containing a conserved domain for the bunyavirus RNA-dependent RNA polymerase (RdRP) superfamily, with 57–80% identity to the RdRP encoded by phenuiviruses in the genus <i>Laulavirus</i>. RNA2 encodes a protein (∼79 kDa) showing sequence similarity (47–63% identity) to the movement protein (MP, a plant viral cell-to-cell movement protein)-like protein (MP-L) encoded by RNA2 of laulaviruses. RNA3 encodes a protein (∼28 kDa) with a conserved domain of the phenuivirid nucleocapsid protein superfamily. Phylogenetic analysis using the RdRPs of various phenuiviruses and other unclassified phenuiviruses showed CjNRSV1 to be grouped with established members of the genus <i>Laulavirus</i>. Our results suggest that CjNRSV1 is a novel fungus-infecting member of the genus <i>Laulavirus</i> in the family <i>Phenuiviridae</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A synthetic TLR4 agonist significantly increases humoral immune responses and the protective ability of an MDCK-cell-derived inactivated H7N9 vaccine in mice 一种合成的 TLR4 激动剂能显著提高小鼠的体液免疫反应和源自 MDCK 细胞的 H7N9 灭活疫苗的保护能力。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-11 DOI: 10.1007/s00705-024-06082-8

Jian Luo, Min Zhang, Qian Ye, Feixia Gao, Wenting Xu, Beibei Li, Qi Wang, Liang Zhao, Wen-Song Tan

{"title":"A synthetic TLR4 agonist significantly increases humoral immune responses and the protective ability of an MDCK-cell-derived inactivated H7N9 vaccine in mice","authors":"Jian Luo, Min Zhang, Qian Ye, Feixia Gao, Wenting Xu, Beibei Li, Qi Wang, Liang Zhao, Wen-Song Tan","doi":"10.1007/s00705-024-06082-8","DOIUrl":"10.1007/s00705-024-06082-8","url":null,"abstract":"<div><p>Antigenically divergent H7N9 viruses pose a potential threat to public health, with the poor immunogenicity of candidate H7N9 vaccines demonstrated in clinical trials underscoring the urgent need for more-effective H7N9 vaccines. In the present study, mice were immunized with various doses of a suspended-MDCK-cell-derived inactivated H7N9 vaccine, which was based on a low-pathogenic H7N9 virus, to assess cross-reactive immunity and cross-protection against antigenically divergent H7N9 viruses. We found that the CRX-527 adjuvant, a synthetic TLR4 agonist, significantly enhanced the humoral immune responses of the suspended-MDCK-cell-derived H7N9 vaccine, with significant antigen-sparing and immune-enhancing effects, including robust virus-specific IgG, hemagglutination-inhibiting (HI), neuraminidase-inhibiting (NI), and virus-neutralizing (VN) antibody responses, which are crucial for protection against influenza virus infection. Moreover, the CRX-527-adjuvanted H7N9 vaccine also elicited cross-protective immunity and cross-protection against a highly pathogenic H7N9 virus with a single vaccination. Notably, NI and VN antibodies might play an important role in cross-protection against lethal influenza virus infections. This study showed that a synthetic TLR4 agonist adjuvant has a potent immunopotentiating effect, which might be considered worth further development as a means of increasing vaccine effectiveness.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 8","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome analysis of a novel hypovirus in the phytopathogenic fungus Monilinia fructicola 植物病原真菌 Monilinia fructicola 中一种新型次病毒的全基因组分析。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-07-11 DOI: 10.1007/s00705-024-06092-6

Kang Zhou, Hui Zhou, Zhimin Tang

引用次数: 0