Identification and complete genome sequence of a new carlavirus from Carica papaya in Jordan

IF 2.5 4区 医学 Q3 VIROLOGY
N. Salem, P. Margaria, M. Abu Muslem, I. Ibrahim, A. Alawidat
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Abstract

A novel carlavirus was discovered in Carica papaya by high-throughput sequencing of a sample collected during field surveys in 2022 in the Jordan Valley, Jordan. The complete genome sequence of 9,169 nt exhibited the typical organization of members of the genus Carlavirus, including a predicted ORF6. Alignment of coat and replication protein aa sequences revealed the closest identity (~94% and ~69%, respectively) to a cucumber vein-clearing virus (CvCV) isolate. According to the ICTV demarcation criteria based on sequence identity values, the virus is a putative new member of the genus Carlavirus, for which the common name “papaya virus B” is proposed. In field surveys, the virus was detected in 20 out of 38 papaya samples with evident virus-like symptoms collected from five fields in the Jordan Valley, showing the occurrence of infections with this novel carlavirus in different cultivated areas.

约旦番木瓜新卡拉病毒的鉴定及全基因组序列分析
通过对2022年在约旦约旦河谷实地调查中收集的样品进行高通量测序,在卡里卡木瓜中发现了一种新的卡拉病毒。9169 nt的全基因组序列显示了卡拉病毒属成员的典型组织,包括预测的ORF6。对外壳和复制蛋白aa序列的比对表明,其与黄瓜清静脉病毒(CvCV)分离物的同源性最接近(分别为~94%和~69%)。根据基于序列同一性值的ICTV划分标准,该病毒推定为卡拉病毒属的新成员,并提出了其共同名称“木瓜病毒B”。在实地调查中,从约旦河谷的5个田地中采集的38个木瓜样品中,有20个样品具有明显的病毒样症状,表明这种新型卡拉病毒感染在不同的栽培地区发生。
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来源期刊
Archives of Virology
Archives of Virology 医学-病毒学
CiteScore
5.10
自引率
7.40%
发文量
324
审稿时长
4.5 months
期刊介绍: Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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