N. Salem, P. Margaria, M. Abu Muslem, I. Ibrahim, A. Alawidat
{"title":"Identification and complete genome sequence of a new carlavirus from Carica papaya in Jordan","authors":"N. Salem, P. Margaria, M. Abu Muslem, I. Ibrahim, A. Alawidat","doi":"10.1007/s00705-025-06309-2","DOIUrl":null,"url":null,"abstract":"<div><p>A novel carlavirus was discovered in <i>Carica papaya</i> by high-throughput sequencing of a sample collected during field surveys in 2022 in the Jordan Valley, Jordan. The complete genome sequence of 9,169 nt exhibited the typical organization of members of the genus <i>Carlavirus</i>, including a predicted ORF6. Alignment of coat and replication protein aa sequences revealed the closest identity (~94% and ~69%, respectively) to a cucumber vein-clearing virus (CvCV) isolate. According to the ICTV demarcation criteria based on sequence identity values, the virus is a putative new member of the genus <i>Carlavirus</i>, for which the common name “papaya virus B” is proposed. In field surveys, the virus was detected in 20 out of 38 papaya samples with evident virus-like symptoms collected from five fields in the Jordan Valley, showing the occurrence of infections with this novel carlavirus in different cultivated areas.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Virology","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s00705-025-06309-2","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
A novel carlavirus was discovered in Carica papaya by high-throughput sequencing of a sample collected during field surveys in 2022 in the Jordan Valley, Jordan. The complete genome sequence of 9,169 nt exhibited the typical organization of members of the genus Carlavirus, including a predicted ORF6. Alignment of coat and replication protein aa sequences revealed the closest identity (~94% and ~69%, respectively) to a cucumber vein-clearing virus (CvCV) isolate. According to the ICTV demarcation criteria based on sequence identity values, the virus is a putative new member of the genus Carlavirus, for which the common name “papaya virus B” is proposed. In field surveys, the virus was detected in 20 out of 38 papaya samples with evident virus-like symptoms collected from five fields in the Jordan Valley, showing the occurrence of infections with this novel carlavirus in different cultivated areas.
期刊介绍:
Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.