针对肺炎克雷伯菌胶囊型K2的强毒噬菌体P12L （Drulisvirus属）的鉴定

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-05-16 DOI:10.1007/s00705-025-06320-7

Wendy Dayang Rollon, Tay Darren Dean, Sharifah Ishaziah Mohd Idris, Nurzafirah Mazlan, Afif Pranaya Jati, Tze Young Thung

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引用次数: 0

摘要

研究了一种感染高粘滞肺炎克雷伯菌胶囊型K2的强毒噬菌体P12L。发现该噬菌体具有足病毒样形态，具有二十面体头部和短尾。它表现出高效的吸附，burst size为183 PFU/cell。病毒基因组是一个线性dsDNA分子，长度为42,343 bp，包含62个假定的开放阅读框（orf）。它缺乏与耐药或毒力因子相关的基因，并编码两个与解聚合酶活性相关的预测结构域。由于解聚合酶可以降解多糖胶囊并促进有效的噬菌体-宿主相互作用，噬菌体P12L显示出作为生物防治剂的潜力。

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Characterization of a virulent phage, P12L (genus Drulisvirus), targeting Klebsiella pneumoniae capsule type K2

A virulent bacteriophage, P12L, infecting hypermucoviscous Klebsiella pneumoniae of capsule-type K2 was characterized. The phage was found to have podovirus-like morphology, with an icosahedral head and a short tail. It exhibited efficient adsorption with a burst size of 183 PFU/cell. The viral genome is a linear dsDNA molecule that is 42,343 bp in length and contains 62 putative open reading frames (ORFs). It lacks genes associated with drug resistance or virulence factors and encodes two predicted domains associated with depolymerase activity. Because depolymerase can degrade polysaccharide capsules and promote efficient phage-host interactions, phage P12L shows potential as a biocontrol agent.

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.