乙型脑炎病毒基因型内嵌合株在小鼠体内的体外生长和致病性评价

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-05-16 DOI:10.1007/s00705-025-06317-2

Shigeru Tajima, Astri Nur Faizah, Daisuke Kobayashi, Kris Cahyo Mulyatno, Sri Subekti, Maria Inge Lusida, Yasuko Mori, Haruhiko Isawa, Kyoko Sawabe, Chang-Kweng Lim

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引用次数: 0

摘要

两株具有越南株VN113和澳大利亚株sw-22-00722-11 5′非编码区和C-prM-E区（5NCME）的重组乙型脑炎病毒（GIV - JEV）基因型内嵌合。Qld （sw-22）以印尼菌株19xba -83- cv为骨干菌株。三株菌斑在Vero细胞中表现出相似的斑块形态和生长速度。与19xba -83- cv相比，携带sw-22 5NCME的病毒对神经的侵袭性较小，但携带VN113 5NCME的病毒的毒力与携带19xba -83- cv的病毒相似。因此，我们的结果表明，sw-22的致病性可能低于其他两个GIV乙脑分离株。

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Evaluation of in vitro growth and pathogenicity in mice of intra-genotype chimeric strains of genotype IV Japanese encephalitis virus

Two recombinant intra-genotype chimeric genotype IV Japanese encephalitis virus (GIV JEV) strains with the 5’ non-coding and C-prM-E regions (5NCME) of the Vietnamese strain VN113 or the Australian strain sw-22-00722-11.Qld (sw-22) were produced using the Indonesian strain 19CxBa-83-Cv as a backbone. The three strains exhibited similar plaque morphology and growth rate in Vero cells. The virus with sw-22 5NCME was less neuroinvasive than 19CxBa-83-Cv, but the virulence of the virus with VN113 5NCME was similar to that of 19CxBa-83-Cv. Thus, our results suggest that the pathogenicity of sw-22 might be lower than that of the other two GIV JEV isolates.

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.