Epidemiological investigation, isolation, and pathogenicity of porcine epidemic diarrhea virus subtype G2c in Sichuan province

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-05-16 DOI:10.1007/s00705-025-06308-3

Min Wang, Mingxiang Li, Guangwen Yan, Hao Li, Jun Zhou, Aiguo Yang

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引用次数: 0

Abstract

Continued outbreaks of porcine epidemic diarrhea are causing serious economic losses to the swine industry in China. To monitor the prevalence, genetic mutations, and pathogenicity of porcine epidemic diarrhea virus (PEDV), 172 samples were collected from eight cities in Sichuan Province from 2020 to 2022. RT-PCR analysis revealed that 25.0% (43/172) of samples were positive for PEDV. Phylogenetic analysis of 17 S gene sequences (encoding the spike protein) showed that G2c was the main genotype circulating in Sichuan. One strain, Leshan-s-2020 (G2a), was identified as a recombinant resulting from inter-lineage recombination between the KM609212/LYG/2015 (G2a) and MianYang-s-2020 (G2a) strains in the S2 domain. In addition, the G2c strain YB2201, which was highly virulent in 4-day-old piglets, was successfully isolated. The results of this study enrich our understanding of the epidemiology of PEDV, the genetic characteristics and pathogenicity of the PEDV strains circulating in China, and the role of recombination in their evolution. These findings may contribute to the development of antigen detection reagents and vaccines.

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猪流行性腹泻病毒G2c亚型在四川省的流行病学调查、分离及致病性

猪流行性腹泻的持续爆发给中国养猪业造成了严重的经济损失。为监测猪流行性腹泻病毒（PEDV）的流行、基因突变和致病性，于2020 - 2022年在四川省8个城市采集172份样本。RT-PCR检测结果显示，25.0%（43/172）的样本PEDV阳性。编码穗蛋白的17个S基因序列的系统发育分析表明，G2c是四川流行的主要基因型。其中乐山-s-2020 （G2a）是由KM609212/LYG/2015 （G2a）与绵阳-s-2020 （G2a）在S2结构域进行系间重组而成的重组菌株。成功分离出对4日龄仔猪具有高毒力的G2c菌株YB2201。本研究结果丰富了我们对PEDV流行病学、中国流行PEDV毒株遗传特征和致病性以及重组在其进化中的作用的认识。这些发现可能有助于抗原检测试剂和疫苗的开发。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.