含有酵母衍生角鲨烯的mf59样佐剂增强了对细胞衍生流感疫苗的体液免疫应答

IF 2.5 4区 医学 Q3 VIROLOGY
Tuanjie Chen, Wenting Xu, Peng Duan, Sijing Jiang, Xue Yang, Hongzhen Cao, Mei Zheng, Jian Luo
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引用次数: 0

摘要

本研究的目的是评估含有酵母衍生角鲨烯的mf59样佐剂(mf59样YD)在基于细胞的四价流感疫苗(QIV)中的佐剂特性,并探讨其潜在的作用机制。含有酵母衍生或鲨鱼衍生角鲨烯的mf59样佐剂被纳入QIV配方。通过酶联免疫吸附试验(elisa)、血凝抑制试验(HI)和微量中和试验(MN)评估小鼠血清中的抗原特异性免疫反应。通过流式细胞术分析小鼠脾生发中心(GC)细胞活化情况,进一步分析佐剂的作用及作用机制。mf59样YD显著提高了小鼠由QIVs诱导的体液免疫应答,特别是对同源和异源病毒亚型的HI和MN抗体滴度。机制上,mf59样YD通过激活GC中的T滤泡辅助细胞(Tfh)和B细胞来增强对流感疫苗的免疫应答。鉴于酵母衍生角鲨烯的可获得性更高,并且发现其佐剂功效与鲨鱼衍生角鲨烯相当,我们建议mf59样YD佐剂是未来流感疫苗的一种有希望的替代佐剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MF59-like adjuvant containing yeast-derived squalene enhances the humoral immune response to cell-derived influenza vaccine

The aims of this study were to assess the adjuvant properties of an MF59-like adjuvant containing yeast-derived squalene (MF59-like YD) in a cell-based quadrivalent influenza vaccine (QIV) and to investigate the potential mechanisms of action. MF59-like adjuvants containing either yeast-derived or shark-derived squalene were incorporated into QIV formulations. Antigen-specific immune responses in mouse serum were evaluated via enzyme-linked immunosorbent assays (ELISAs), hemagglutination inhibition (HI) assays, and microneutralization (MN) assays. The effects and mechanisms of action of the adjuvants were further analyzed by analyzing mouse spleen germinal center (GC) cell activation via flow cytometry. MF59-like YD significantly increased the humoral immune responses induced by QIVs in mice, in particular, the titers of HI and MN antibodies against homologous and heterologous virus subtypes. Mechanistically, MF59-like YD increased the immune response to influenza vaccines by activating T follicular helper (Tfh) and B cells in the GC. Given the greater availability of yeast-derived squalene and the finding that its adjuvant efficacy was comparable to that of shark-derived squalene, we propose that the MF59-like YD adjuvant is a promising alternative adjuvant for future influenza vaccines.

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来源期刊
Archives of Virology
Archives of Virology 医学-病毒学
CiteScore
5.10
自引率
7.40%
发文量
324
审稿时长
4.5 months
期刊介绍: Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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