两种不同蚕豆染色病毒分离株的全基因组序列

IF 2.5 4区 医学 Q3 VIROLOGY
Fumino Nito, Yuya Imamura, Yuji Fujiwara, Takayuki Matsuura, Hironobu Yanagisawa
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引用次数: 0

摘要

这是两种蚕豆染色病毒(BBSV;Comovirus属)株-突尼斯(RNA1, 5,965 nt;RNA2, 3,467 nt)和叙利亚(RNA1, 5,976 nt;RNA2, 3,449 nt) -采用下一代测序(NGS)和cDNA末端快速扩增(RACE)测定。这些菌株在RNA1和RNA2上的核苷酸序列同源性分别为79.2%和71.6%。预测的氨基酸序列在蛋白酶聚合酶和外壳蛋白上的同源性分别为95.2%和93.9%,表明这些菌株属于科病毒属同一种。系统发育分析表明,这两株毒株与红三叶草斑驳病毒(comvirus属)亲缘关系密切。这是首次报道的两株BBSV的全基因组序列。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Complete genome sequences of two different broad bean stain virus isolates

Here, the complete genome sequences of two broad bean stain virus (BBSV; genus Comovirus) strains – Tunisia (RNA1, 5,965 nt; RNA2, 3,467 nt) and Syria (RNA1, 5,976 nt; RNA2, 3,449 nt) – were determined using next-generation sequencing (NGS) and rapid amplification of cDNA ends (RACE). These strains showed 79.2% and 71.6% nucleotide sequence identity to each other in RNA1 and RNA2, respectively. The predicted amino acid sequence identity was 95.2% in the proteinase-polymerase and 93.9% in the coat protein, indicating that these strains belong to the same species in the genus Comovirus. Phylogenetic analysis showed that both strains are closely related to red clover mottle virus (genus Comovirus). This is the first report of the complete genome sequences of two BBSV strains.

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来源期刊
Archives of Virology
Archives of Virology 医学-病毒学
CiteScore
5.10
自引率
7.40%
发文量
324
审稿时长
4.5 months
期刊介绍: Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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