Molecular characterization and epidemiological insights into serotypes of foot-and-mouth disease virus in Pakistan

IF 2.5 4区 医学 Q3 VIROLOGY
Syeda Anam Masood Gardezi, Masood Rabbani, Muhammad Hassan Mushtaq, Muhammad Zubair Shabbir
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引用次数: 0

Abstract

Foot-and-mouth disease virus (FMDV) causes a highly infectious transboundary disease in cloven-hoofed animals. However, there is limited information on comparative genomic and evolutionary analysis of FMDV strains reported in Pakistan. In the current study, we investigated a few disease outbreaks in Pakistan and determined the complete genome sequences of three isolates belonging to different serotypes: O, A, and Asia-1. Comparative genomic analysis showed a close relationship between the FMDV strains from this study and those reported in neighboring Asian countries. Phylogenetic analysis based on the complete genome and VP1 gene sequences revealed that serotype O clustered within the Pak-98 lineage, serotype A within genotype I, and serotype Asia-1 within group 5, grouping with strains from Pakistan, India, and China. Potential negatively selected sites were identified in the region encoding the structural proteins VP4, VP3, VP2, and VP1, with the most episodic diversifying section observed in the VP1 and VP3 regions. Substantial variation was observed in polymorphic and monomorphic sites, with the highest diversity observed in the VP1 protein of serotype O viruses. No evidence of recombination events was found in the FMDV strains from this study. Our findings indicate the cocirculation of multiple serotypes and sublineages of FMDV in Pakistan, underscoring the potential for the emergence of new variants. This study adds to the existing knowledge on the genetic variation and evolutionary dynamics of FMDV serotypes in Pakistan, providing valuable insights for the development of effective vaccines and improved disease control strategies.

巴基斯坦口蹄疫病毒血清型的分子特征和流行病学见解
口蹄疫病毒(FMDV)是一种在偶蹄动物中具有高度传染性的跨界疾病。然而,关于巴基斯坦报告的口蹄疫毒株的比较基因组和进化分析的信息有限。在目前的研究中,我们调查了巴基斯坦的一些疾病暴发,并确定了属于不同血清型(O、a和Asia-1)的三种分离株的全基因组序列。比较基因组分析表明,本研究的FMDV毒株与亚洲邻国报道的FMDV毒株关系密切。基于全基因组和VP1基因序列的系统发育分析显示,血清O型聚集在Pak-98谱系中,血清A型聚集在基因I谱系中,血清Asia-1聚集在基因5谱系中,与来自巴基斯坦、印度和中国的菌株分群。在编码结构蛋白VP4、VP3、VP2和VP1的区域发现了潜在的负选择位点,在VP1和VP3区域观察到最幕式多样化的部分。在多态和单态位点上观察到大量的差异,其中在血清O型病毒的VP1蛋白中观察到最高的多样性。本研究未发现FMDV毒株发生重组事件的证据。我们的研究结果表明,FMDV的多种血清型和亚系在巴基斯坦共循环,强调了新变体出现的可能性。这项研究增加了关于巴基斯坦口蹄疫血清型遗传变异和进化动力学的现有知识,为开发有效疫苗和改进疾病控制策略提供了有价值的见解。
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来源期刊
Archives of Virology
Archives of Virology 医学-病毒学
CiteScore
5.10
自引率
7.40%
发文量
324
审稿时长
4.5 months
期刊介绍: Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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