Physiologia plantarum最新文献

{"title":"Correction to \"Plant Proximity Reduces Seed Yield in Arabidopsis Plants by Decreasing the Number of Ovule Primordia\".","authors":"","doi":"10.1111/ppl.70317","DOIUrl":"https://doi.org/10.1111/ppl.70317","url":null,"abstract":"","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70317"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Overexcitation of Photosystem II on Chlorophyll Fluorescence Quenching Parameters in Arabidopsis thaliana State Transition Mutants.","authors":"Anja Krieger-Liszkay","doi":"10.1111/ppl.70335","DOIUrl":"10.1111/ppl.70335","url":null,"abstract":"<p><p>Photosynthesis is driven by light absorbed by pigments situated in the antenna of the photosystems. Depending on the light quality, there is a difference in the absorption of Photosystems I and II. A balanced light absorption between both photosystems is required for optimal photosynthesis. State transitions are the acclimation response of the photosynthetic apparatus that allows adjusting the antenna size of the two photosystems. The dependency of chlorophyll fluorescence quenching parameters on the light quality was investigated in Arabidopsis wild type and state transition mutants. The parameter qL, indicating the fraction of open Photosystem II reaction centers, showed a large dependency on the quality of the actinic light in state transition mutants incapable of performing the transition to State 2. While the difference between wild type and these mutants was small in blue or red light, the difference was much more pronounced when cyan or green light was used instead. The phosphatase mutant pph, with the mobile part of the light harvesting Complex II locked at Photosystem I, the chlorophyll b-less mutant ch1, and phototropin mutants phot1 and phot2 affected in chloroplast movement did not show such low qL in cyan or green light. This shows that overexcitation of Photosystem II was responsible for the low qL in Arabidopsis state transition mutants stn7, nsi1, and nsi2.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70335"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12172140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rice RING E3 Ligase OsRFP45 Negatively Regulates Salt Tolerance by Modulating Na⁺/K⁺ Transporter Genes.","authors":"Min Seok Choi, Jong Ho Kim, Cheol Seong Jang","doi":"10.1111/ppl.70327","DOIUrl":"10.1111/ppl.70327","url":null,"abstract":"<p><p>Salinity stress is a major environmental challenge affecting global rice production by disrupting ion homeostasis and inducing oxidative damage. We characterized Oryza sativa RING Finger Protein 45 (OsRFP45), a RING-v-type E3 ubiquitin ligase, and investigated its role in the salt stress response in rice. OsRFP45-overexpressing (OE) and CRISPR/Cas9-mediated knockout (KO) rice lines were generated to examine their physiological, biochemical, and molecular responses to salt stress. While no significant differences were observed among genotypes under normal conditions, OsRFP45-OE plants exhibited severe growth retardation, high Na⁺ accumulation, low K⁺ retention, increased oxidative stress, and reduced osmotic adaptation under 100 mM NaCl, demonstrating hypersensitivity to salinity. In contrast, OsRFP45-KO plants displayed enhanced salt tolerance, maintained low Na⁺ content, a balanced Na⁺/K⁺ ratio, reduced reactive oxygen species accumulation, and increased proline and soluble sugar levels. Quantitative RT-PCR analysis revealed that the expression of OsRFP45 negatively modulated the expression of key Na⁺ and K⁺ transporters, including OsHKT1;5, OsHKT2;1, OsNHX1, and OsSOS1. In OsRFP45-KO plants, enhanced Na⁺ exclusion and K⁺ retention contributed to improved ionic homeostasis under salt stress. Additionally, in vitro ubiquitination assays confirmed the E3 ligase activity of OsRFP45, indicating its potential role in protein turnover during adaptation to stress. Taken together, our findings suggest that OsRFP45 functions as a negative regulator of salt tolerance by modulating ion transport and oxidative stress response. Understanding the molecular role of OsRFP45 may provide a promising strategy for developing salt-tolerant rice cultivars.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70327"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal Variations in Sugar Accumulation Patterns of Dendrobium officinale: Integrated Analysis via Machine Learning, Physiology, and Transcriptomics.","authors":"Jing Li, Yuanju Zhang, Yingyue Hou, Rong Zhou, Yang Lu, Guangying Du","doi":"10.1111/ppl.70334","DOIUrl":"10.1111/ppl.70334","url":null,"abstract":"<p><p>The genus Dendrobium, the largest within the Orchidaceae family, includes Dendrobium officinale, a dual-purpose medicinal and edible functional food experiencing substantial market demand in Asia. The accumulation patterns and molecular regulatory networks of sugars in its stems and leaves are influenced by seasonal temperature and humidity variations. Understanding these patterns and mechanisms is essential for ensuring high-quality D. officinale production. Based on dynamic monitoring and machine learning models over 2 years involving 2318 data points, this study discovered seasonal variations in glucose, sucrose, fructose, mannose, galactose, and galacturonic acid in stems and leaves conformed to a polynomial regression model (R² = 0.742). The model revealed sugar content in stems primarily accumulated from October to April of the following year, with a rapid accumulation from October to February of the subsequent year. Combined with transcriptomic profiling at three critical growth stages, the differentially expressed genes related to sugar metabolism and abiotic stress in leaves and stems were mainly enriched in pathways such as plant-pathogen interaction, photosynthesis, and starch and sucrose metabolism. Additionally, 15 hub genes (e.g., RPS5, RPL23A, PSBO, etc.) were screened using weighted gene co-expression correlation network analysis, which may regulate photosynthesis-related pathways by responding to low-temperature and drought stresses, thereby facilitating normal sugar metabolism in D. officinale to adapt to environmental changes. In summary, these findings elucidate the seasonal variation models and molecular regulatory mechanisms of six sugars in D. officinale, offering scientific guidance for its harvesting and molecular breeding.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70334"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Putrescine eases saline stress by regulating biochemicals, antioxidative enzymes, and osmolyte balance in hydroponic strawberries (cv. Albion).","authors":"Ferhad Muradoğlu, Şeyma Batur, Mirmahmud Hasanov, Emrah Güler","doi":"10.1111/ppl.70259","DOIUrl":"https://doi.org/10.1111/ppl.70259","url":null,"abstract":"<p><p>Salinity is a significant abiotic stress factor that causes considerable damage to many plants through various mechanisms. In this study, the ameliorative effect of putrescine (100, 150, and 200 ppm) on salinity stress (1 g L^-1 NaCl) was investigated in strawberry cv. Albion grown in hydroponic culture. Results showed that putrescine provided root development comparable to control under salt stress. Chlorophyll a and b levels were slightly enhanced by putrescine, while the Chlorophyll a/b ratio was significantly improved. The total phenolics, carbohydrates, anthocyanin, and protein were also raised by putrescine treatments under saline conditions. The antioxidant mechanism was also fortified by putrescine treatment, evidenced by the increased DPPH scavenging and the rise in the antioxidant enzymes, SOD and CAT. The oxidative substances, particularly MDA, notably decreased by rising putrescine doses. Moreover, putrescine treatments positively regulated root-to-shoot osmolyte homeostasis with a diverse effect mechanism according to applied doses. Overall, this study provides a comprehensive insight into the effects of putrescine on the saline stress mechanism in strawberries and suggests it as a favourable biogenic agent.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70259"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haematococcus pluvialis bionanoparticles boost maize seedling health, serving as a sustainable seed priming agent and biostimulant for agriculture.","authors":"Nahid Rafiei, Hossein Alishah Aratboni, Leandro Luis Lavandosque, Clíssia Barboza Mastrangelo, Welinton Yoshio Hirai, Lucianne Ferreira Paes de Oliveira, Gabriel Luiz Padoan Gonçalves, José Lavres, Mônica Lanzoni Rossi, Adriana Pinheiro Martinelli, Simone Possedente de Lira, Seyed Abdolreza Kazemeini, Flavia Vischi Winck","doi":"10.1111/ppl.70245","DOIUrl":"https://doi.org/10.1111/ppl.70245","url":null,"abstract":"<p><p>The rising frequency of extreme climate events requires sustainable strategies to secure food production. Environmental stress impacts seed germination and seedling development, posing a significant agricultural challenge. To address this, we developed and applied iron-based nanoparticles (Bio-NPs) synthesized through green biosynthesis from Haematococcus pluvialis, a microalga rich in antioxidants like astaxanthin. These Bio-NPs, approximately 21 nm in diameter and characterized by a negative surface charge, were used as priming agents for maize seeds. Their effects on physiological traits were analyzed with multispectral imaging, showing enhanced normalized difference vegetation index (NDVI) and chlorophyll levels in maize seedlings, highlighting Bio-NPs as effective biostimulants. Among the tested concentrations, 6 mM Bio-NPs yielded the most substantial improvements in seedling health compared to unprimed and hydro-primed groups. Importantly, in vitro studies confirmed that Bio-NPs had no harmful effects on beneficial bacteria and fungi of agronomic importance, underscoring their safety. Although the exact biological pathways responsible for these enhancements are yet to be fully understood, further research into plant responses to Bio-NPs could yield new insights into plant biostimulation. Bio-NPs thus hold promises for strengthening seedling resilience under extreme environmental scenarios, currently observed due to global climate change, offering a safe, sustainable approach to agricultural enhancement. By leveraging microalgae-based biostimulants, this work advances seed priming technology, fostering crop resilience and supporting environmentally friendly agricultural practices.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70245"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoprotective Responses of Cotton Non-Leaf Green Tissues to Short-Term Low-Temperature Stress.","authors":"Pei Yang, Shuhao Lv, Fubin Liang, Jingshan Tian, Yali Zhang, Chuangdao Jiang, Wangfeng Zhang","doi":"10.1111/ppl.70246","DOIUrl":"https://doi.org/10.1111/ppl.70246","url":null,"abstract":"<p><p>Short-term low-temperature stress during the flowering and boll-forming stages significantly inhibits cotton growth and yield. While leaf photosynthesis is a major contributor to cotton yield, non-leaf green tissues (bracts and bolls) also play a crucial role. However, the differential impacts of short-term low-temperature stress on the photosynthetic activity of these tissues and their protective mechanisms remain underexplored. In this study, the cotton cultivar \"Xinluzao 45\" was subjected to three temperature regimes in a controlled climate chamber: Control (30°C/20°C), T1 (16°C/10°C), and T2 (12°C/8°C). After two days of treatment, pigment content, photosynthetic activity, light energy distribution, and cyclic electron flow (CEF) around photosystem I were analyzed in both leaf and non-leaf green tissues. Results showed that short-term low-temperature stress decreased the maximum photochemical efficiency of PSII (Fv/Fm), actual photochemical efficiency [Y(II)], and maximum photo-oxidizable P700 (Pm) in both leaf and non-leaf green tissues. Compared to leaves and bracts, cotton boll shells exhibited greater photosynthetic stability, which was related to their higher carotenoid (Car) content, larger plastoquinone (PQ) pool, and enhanced CEF capacity under stress. Chemical inhibitor experiments (using antimycin A and rotenone) indicated that PGRL1/PGR5-mediated CEF plays a more critical role than the NDH pathway in cotton's response to short-term low-temperature stress. These findings highlight the distinct photoprotective advantages of cotton bolls and provide insights for breeding low-temperature-tolerant cultivars and improving management practices.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70246"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental and safety aspects of nanotechnology in genetically modified crops for sustainable agriculture.","authors":"Muhammad Nadeem, Noman Shakoor, Muhammad Adeel, Imran Azeem, Muhammad Zain, Yuanbo Li, Usama Zaheer, Jazib Javed, Rabia Khalid, Peng Zhang, Iseult Lynch, Yukui Rui","doi":"10.1111/ppl.70239","DOIUrl":"https://doi.org/10.1111/ppl.70239","url":null,"abstract":"<p><p>The rising global demand for food poses a significant threat to environmental health through both biotic (e.g., pests, pathogens) and abiotic (e.g., drought, salinity) stresses. Therefore, the adoption of innovative strategies is essential to ensure the sustainability of agricultural practices and to enhance crop resilience against environmental challenges. This review investigates how the integration of nanotechnology with genetically modified (GM) crops can offer solutions to agricultural challenges by improving crop resilience and productivity. While genetic modification has faced limitations in achieving consistent results due to environmental variability and species-specific differences, nanotechnology has emerged as a transformative tool to enhance GM crop performance. In this study we critically explore the underlying mechanisms of combining nanotechnology with GM crops to enhance plant growth and development and their resilience against biotic and abiotic stresses. Furthermore, nanotechnology also play a crucial role in targeted gene delivery, precise genome editing, and controlled regulation of gene expression in GM plant cells. Overall, the emerging role of nanotechnology in GM crops is paving the way for innovative solutions in agriculture. By leveraging nanotechnology, researchers are exploring novel approaches to enhance productivity, combat plant diseases, and improve plant resilience to environmental stress for sustainable agriculture. Furthermore, in this review we also highlighted the environmental impacts and safety issues associated with using nanotechnology in crops in order to establish more resilient and sustainable farming practices.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70239"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen Peroxide Positively Regulates Phosphate Starvation Responses in Rice.","authors":"Xu Yang, Guangda Ding, Hongmei Cai, Sheliang Wang, Xu Wang, Suren Deng, Chuang Wang","doi":"10.1111/ppl.70264","DOIUrl":"10.1111/ppl.70264","url":null,"abstract":"<p><p>Phosphorus is an essential macronutrient for plant growth and development. Under phosphate (Pi) starvation conditions, plants activate a series of adaptive responses, among which reactive oxygen species (ROS) accumulation in root tissues represents a notable yet poorly characterized phenomenon. This study investigated the regulatory role of hydrogen peroxide (H₂O₂) in rice adaptation to Pi deficiency through pharmacological intervention using potassium iodide (KI), a specific H₂O₂ scavenger. Physiological analysis revealed that root-specific H₂O₂ depletion via KI treatment significantly impaired both Pi uptake and root growth under Pi-deficient conditions. Transcriptomic profiling demonstrated that H₂O₂ elimination substantially modulated the expression of 196 Pi starvation-responsive genes, particularly those involved in SPX-mediated phosphate sensing, extracellular acid phosphatases (APase) biosynthesis, high-affinity phosphate transporters, lipid metabolism enzymes, and redox homeostasis maintenance. Subsequent biochemical validation confirmed that both KI and diphenyleneiodonium (DPI) treatments suppressed Pi-starvation-induced APase activity and compromised Pi uptake ability. Notably, comparative analysis with the phr1/2/3 triple mutant revealed a 24% overlap in differentially expressed genes between H₂O₂ and PHR-deficient plants, with 90% of shared genes exhibiting congruent expression patterns. These findings collectively establish that H₂O₂ serves as a pivotal signaling mediator in the Pi starvation regulatory network, orchestrating metabolic reprogramming and developmental adaptation to Pi stress in rice.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70264"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Pentatricopeptide Repeat Protein Restores Fertility in Tadukan-Type Cytoplasmic Male Sterile Rice via the Cleavage of the Mitochondrial orf312 RNA.","authors":"Ayumu Takatsuka, Yuko Iwai, Hakim Mireau, Tomohiko Kazama, Hiroyuki Ichida, Tomoko Abe, Keisuke Igarashi, Kinya Toriyama","doi":"10.1111/ppl.70308","DOIUrl":"10.1111/ppl.70308","url":null,"abstract":"<p><p>Cytoplasmic male sterility (CMS) is associated with the inhibition of pollen and/or anther development regulated by a CMS-causing gene in the mitochondrial genome; it is a useful trait for preventing self-pollination and producing F₁ hybrids, which can boost crop yields. Pollen and/or anther development can be recovered by the action of the RESTORER OF FERTILITY (Rf) gene, a nuclear-encoded gene. Most reported Rf genes encode pentatricopeptide repeat (PPR) proteins, which bind to RNA and promote RNA processing of the respective CMS-causing gene. In this study, we report the map-based cloning of the Rf gene (Rfta) for Tadukan-type CMS (TA-CMS) in rice (Oryza sativa L.), with anther dehiscence and seed setting inhibited by the mitochondrial gene orf312. The Rfta locus was delimited to a region comprising 10 PPR genes forming a cluster on chromosome 10. The complementation test revealed that the introduction of a PPR gene, PPR796, into the TA-CMS line resulted in the recovery of the anther dehiscence and seed setting. RNA-gel blot analysis and the determination of 3' ends of the orf312 RNA confirmed the PPR796-mediated cleavage of the orf312 RNA in the transgenic TA-CMS line. Furthermore, RNA gel electrophoretic mobility shift assays revealed that the recombinant PPR796 protein bound to the 3' side of the orf312 RNA in vitro. We concluded that RFta/PPR796 binds to orf312 RNA and promotes RNA cleavage to restore fertility in TA-CMS.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70308"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}