Physiologia plantarum最新文献_第8页

Genetic diversity and population structure analysis of short-day onions using molecular markers in association with resistance to Fusarium basal rot. 短日洋葱抗枯萎病遗传多样性及群体结构分析。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70042

SaeidReza Poursakhi, Hossein Ali Asadi-Gharneh, Mehdi Nasr-Esfahani, Zahra Abbasi, Hamed Hassanzadeh Khankahdani

{"title":"Genetic diversity and population structure analysis of short-day onions using molecular markers in association with resistance to Fusarium basal rot.","authors":"SaeidReza Poursakhi, Hossein Ali Asadi-Gharneh, Mehdi Nasr-Esfahani, Zahra Abbasi, Hamed Hassanzadeh Khankahdani","doi":"10.1111/ppl.70042","DOIUrl":"https://doi.org/10.1111/ppl.70042","url":null,"abstract":"In this research, we analyzed Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Sequence-related amplified polymorphism (SRAP) markers to evaluate the genetic diversity of eighteen different onion genotypes with various resistant levels to FOC. The results showed that the polymorphism means between RAPD primers was 61.11 to 81.81%; ISSR primers, 62.50 to 81.81%; and SRAP primers, 56.25 to 76.25%. Overall, by assessing MI, PIC, I and H indices, indicating the best thrive in evaluating the genetic diversity of the related onion populations. There is a significant correlation between the generated dendrograms based on similarity matrices. The classification pattern in dendrograms shows a corresponding correlation with the FOC disease severity bunches. So in all three markers studied, 'Saba' and 'Saba - HS', the most resistant ones to FOC disease, were grouped in a branch, and the 'Sahar - HS' and 'Golden Eye', the most susceptible ones were also grouped in another branch separately. This finding indicates that predominant primers act as markers linked to resistance gene(s) against FOC, which can be used to select onions resistant to FOC disease in any breeding scheme.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70042"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary dynamics of nitrate uptake, assimilation, and signalling in plants: adapting to a changing environment. 植物对硝酸盐吸收、同化和信号传导的进化动力学：适应不断变化的环境。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70069

Xiaojia Zhang, Shufeng Bai, Hui Min, Yuxuan Cui, Yibo Sun, Yulong Feng

{"title":"Evolutionary dynamics of nitrate uptake, assimilation, and signalling in plants: adapting to a changing environment.","authors":"Xiaojia Zhang, Shufeng Bai, Hui Min, Yuxuan Cui, Yibo Sun, Yulong Feng","doi":"10.1111/ppl.70069","DOIUrl":"https://doi.org/10.1111/ppl.70069","url":null,"abstract":"Nitrogen (N) is a crucial macronutrient for plant growth, with nitrate as a primary inorganic N source for most plants. Beyond its role as a nutrient, nitrate also functions as a signalling molecule, influencing plant morphogenetic development. While nitrate utilization and signalling mechanisms have been extensively studied in model plants, the origin, evolution, and diversification of core components in nitrate uptake, assimilation, and signalling remain largely unexplored. In our investigation, we discovered that deep sea algae living in low nitrate conditions developed a high-affinity transport system (HATS) for nitrate uptake and a pathway of nitrate primary assimilation (NR-NiR-GS-GOGAT). In contrast, low-affinity transport systems (LATS) and the plastid GS originated from the ancestors of land and seed plants, respectively. These adaptations facilitated amino acid acquisition as plants conquered terrestrial environments. Furthermore, the intricate nitrate signalling, relying on NRT1.1 and NLP7, evolved stepwise, potentially establishing systematic regulation in bryophytes for self-regulation under complex terrestrial nitrate environments. As plants underwent terrestrialization, they underwent adaptive changes to thrive in dynamic nitrate environments, continually enhancing their nitrate uptake, assimilation, and signal transduction abilities.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70069"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of stored carbon for new organ development in apple saplings in early spring for two consecutive years after ¹³C labelling. 13C标记后连续两年苹果幼树早春贮藏碳对新器官发育的影响。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70077

Shogo Imada, Yasuhiro Tako

{"title":"Use of stored carbon for new organ development in apple saplings in early spring for two consecutive years after 13C labelling.","authors":"Shogo Imada, Yasuhiro Tako","doi":"10.1111/ppl.70077","DOIUrl":"https://doi.org/10.1111/ppl.70077","url":null,"abstract":"The use of stored carbon is essential for new organ development in deciduous trees during early spring. However, the contribution of carbon to the development of new organs in early spring of subsequent years is not well understood. Using a 13C labelling approach, we investigated the reallocation of assimilated carbon into new aboveground organs on apple (Malus domestica) saplings in the following two years. Eight three-year-old potted saplings were exposed to 13CO2 in an exposure chamber on each of eight different dates during the growth season. Some of the trees were harvested in the late autumn of the same year. The remaining trees were transferred to a field and cultivated during the two following growing seasons. We directly showed that the assimilated 13C was used to develop terminal and flower buds for two consecutive years after labelling. The proportions of the concentration of 13C remobilized to the terminal and flower buds in the second year were 5 and 24% of those in the first year after labelling, respectively. The concentration of assimilated 13C was higher in the terminal buds than in the flower buds in the first year after the labelling, while opposite results were found in the second year. This study demonstrates that the stored carbon used for the development of new organs was a mixture of recent- and old-stored carbon and indicates that recently-stored carbon was preferentially used to develop new organs. We also indicated that the stored carbon was remobilized to flower buds during development.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70077"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The spatiotemporal changes of metabolites in Pinellia ternata at different development stages by MALDI-MSI. 利用MALDI-MSI研究半夏不同发育阶段代谢物的时空变化。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70049

Yifei Pei, Xue Feng, Ziyi Liu, Jialei Chen, Jialu Wang, Xiangyu Zhang, Haitao Liu, Xiwen Li

{"title":"The spatiotemporal changes of metabolites in Pinellia ternata at different development stages by MALDI-MSI.","authors":"Yifei Pei, Xue Feng, Ziyi Liu, Jialei Chen, Jialu Wang, Xiangyu Zhang, Haitao Liu, Xiwen Li","doi":"10.1111/ppl.70049","DOIUrl":"https://doi.org/10.1111/ppl.70049","url":null,"abstract":"Pinellia ternata is an herb species in the Pinellia genus with significant economic value due to its medicinal properties. Understanding the accumulation and spatial distribution characteristics of metabolites during the development of the medicinal part, the rhizome of P. ternata (PR), provides a basis for targeted metabolic regulation and quality evaluation. In this study, we used matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI) and MS/MS to analyse metabolites at 5 representative stages (S1 to S5) of rhizome development in cross and longitudinal sections of the rhizome. A total of 168 metabolites were detected, with 13 being metabolites previously reported in PR. Additionally, Venn analysis revealed 12 bioactive differential metabolites during the growth process. Their spatial distribution and composition were analyzed, showing that alkaloids and amino acids were significantly distributed throughout the entire region and had higher relative contents compared to other metabolites. Flavonoids were more distributed in the outer regions of PR, potentially playing a greater role in combating biotic or abiotic stresses. Specifically, in cross-sections, arginine, nicotinamide, and 2-pentylpyridine showed a clear trend of accumulation from the outer to the inner from S1 to S5, while trigonelline, adenosine, cytidine, 3,4-dihydroxycinnamyl alcohol, raffinose, choline alfoscerate, liquiritin, and apii exhibited the opposite trend. For longitudinal sections, trigonelline, 2-pentylpyridine, choline alfoscerate and baicalein showed a trend of accumulation from the area of bud end to the far region during S1 to S5, while arginine showed opposite distribution trends. These findings deepen our understanding of the metabolic processes involved in the development of PR and have potential implications for variety improvement and quality control.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70049"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AsGAD1 cloned from creeping bentgrass modulates cadmium tolerance of Arabidopsis thaliana by remodelling membrane lipids and cadmium uptake, transport and chelation. 从匍匐弯草中克隆的AsGAD1通过重塑膜脂和镉的吸收、转运和螯合来调节拟南芥的镉耐受性。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70063

Yilin Di, Yiqin Cao, Dandan Peng, Ying Liu, Zhou Li

{"title":"AsGAD1 cloned from creeping bentgrass modulates cadmium tolerance of Arabidopsis thaliana by remodelling membrane lipids and cadmium uptake, transport and chelation.","authors":"Yilin Di, Yiqin Cao, Dandan Peng, Ying Liu, Zhou Li","doi":"10.1111/ppl.70063","DOIUrl":"https://doi.org/10.1111/ppl.70063","url":null,"abstract":"The gene GAD1 encodes a glutamate decarboxylase, which is a rate-limiting enzyme for the biosynthesis of endogenous γ-aminobutyrate acid (GABA), but a potential role of GAD1 in regulating cadmium (Cd) tolerance needs to be further elucidated in plants. The objective of this study was to investigate Cd tolerance of creeping bentgrass (Agrostis stolonifera) and transgenic yeast (Saccharomyces cerevisiae) or Arabidopsis thaliana overexpressing AsGAD1. The Cd-tolerant creeping bentgrass cultivar LOFTSL-93 accumulated more endogenous GABA in relation to a significant upregulation of AsGAD1 in leaf and root than the Cd-sensitive W66569 in response to Cd stress. The overexpression of AsGAD1 significantly enhanced Cd tolerance of yeast or A. thaliana associated with improved endogenous GABA content, low oxidative damage, and high cell membrane stability and photochemical efficiency. Compared with wild type, AsGAD1-overexpressing plants or the atgad1 mutant maintained significantly lower or higher Cd content in leaf and root by down-regulating or up-regulating transcript levels of AtNRAMP1/2/3/4/5 and AtZIP1/2, respectively. Moreover, overexpression of AsGAD1 significantly up-regulated transcript levels of AtHMA1/3, contributing to better Cd compartmentalization from chloroplast into cytoplasm and then into vacuoles. AsGAD1 overexpression also induced expressions of AsMT1A/1B/1C/2/3, AsGSH1/2, and AsPCS1/2, indicating better capacity of Cd chelation in cytosol and vacuoles for Cd detoxification. Hence, AsGAD1-regulated detoxification mechanism of Cd could be related to Cd uptake, transport, and chelation. In addition, lipid contents (PC, PG, and DGDG) and the DGDG/MGDG and PC/PG ratios were improved by the AsGAD1 overexpression, which favors membrane stability and functionality under Cd stress. These findings provide new insight into the regulatory role of GAD1 in Cd tolerance in plants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70063"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inositol polyphosphates regulate resilient mechanisms in the green alga Chlamydomonas reinhardtii to adapt to extreme nutrient conditions. 肌醇多磷酸调节绿藻莱茵衣藻适应极端营养条件的弹性机制。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70089

Rodrigo Bedera-García, María Elena García-Gómez, José María Personat, Inmaculada Couso

{"title":"Inositol polyphosphates regulate resilient mechanisms in the green alga Chlamydomonas reinhardtii to adapt to extreme nutrient conditions.","authors":"Rodrigo Bedera-García, María Elena García-Gómez, José María Personat, Inmaculada Couso","doi":"10.1111/ppl.70089","DOIUrl":"https://doi.org/10.1111/ppl.70089","url":null,"abstract":"In the context of climate changing environments, microalgae can be excellent organisms to understand molecular mechanisms that activate survival strategies under stress. Chlamydomonas reinhardtii signalling mutants are extremely useful to decipher which strategies photosynthetic organisms use to cope with changeable environments. The mutant vip1-1 has an altered profile of pyroinositol polyphosphates (PP-InsPs), which are signalling molecules present in all eukaryotes and have been connected to P signalling in other organisms including plants, but their implications in other nutrient signalling are still under evaluation. In this study, we conducted prolonged starvation in WT and vip1-1 Chlamydomonas cells. After N and P had been consumed, they showed important differences in the levels of chlorophyll, photosystem II (PSII) activity and ultrastructural morphology, including differences in the cell size and cell division. Metabolomic analysis under these conditions revealed an overall decrease in different organic compounds such as amino acids, including arginine and its precursors and tryptophan, which is considered a signalling molecule itself in plants. In addition, we observed significant differences in RNA levels of genes related to N assimilation that are under the control of the NIT2 transcription factor. These data are of important relevance in understanding the signalling role of PP-InsPs in nutrient sensing, especially regarding N, which has not directly been connected to these molecules in green organisms before. Additionally, the PP-InsPs regulation over cell size and photosynthesis supports novel strategies for the generation of resilient strains, expanding the biotechnological applications of green microalgae.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70089"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New insights into rice phenology: discovering the effect of insolation on heading response. 水稻物候学的新认识：日晒对抽穗反应的影响。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70132

Ju-Hee Kim, So-Hye Jo, Ji-Hyeon Moon, Seo-Yeong Yang, Jae-Kyeong Baek, Yeong-Seo Song, Ji-Young Shon, Hyeon-Seok Lee

{"title":"New insights into rice phenology: discovering the effect of insolation on heading response.","authors":"Ju-Hee Kim, So-Hye Jo, Ji-Hyeon Moon, Seo-Yeong Yang, Jae-Kyeong Baek, Yeong-Seo Song, Ji-Young Shon, Hyeon-Seok Lee","doi":"10.1111/ppl.70132","DOIUrl":"10.1111/ppl.70132","url":null,"abstract":"Precise growth management is required for climate-smart and sustainable crop production in response to climate change, with the heading stage being the most important. Research on the control of heading in rice (Oryza sativa) has mainly focused on day length and temperature; however, research on the effects of insolation is limited. Therefore, this study analyzed the differences in rice growth and heading responses under different light intensity and temperature conditions. Five early-maturing and seven medium-late-maturing rice varieties were used for each japonica heading ecology type. Our results showed that leaf age development, an indirect measure of rice phenological development, was inhibited under low light intensity and low-temperature conditions. Accordingly, the heading date was also delayed by approximately 18 days at low temperatures and 21 days at low light intensity, with no difference among ecotypes. We also found an interaction between temperature and light intensity, with the light intensity-mediated delay in heading date being affected more by high temperatures. This study demonstrated that light intensity and temperature have a major effect on heading date variation, suggesting that the impact of insolation must be considered for the accurate prediction of heading stage variation. These results could shed new light on rice phenology research and contribute to the implementation of precision agriculture.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70132"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11836917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data-driven resources and computational tools in non-model plant species. 非模式植物物种的数据驱动资源和计算工具。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70133

Nathaniel R Street

引用次数: 0

Substitutability evaluation and resource utilization of non-medicinal parts of Ardisia crenata Sims, a traditional ethnic medicine. 民族传统药材白荆非药用部分的可替代性评价及资源利用

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70068

Jie Peng, Sha Liu, Fusong Liu, Lin Qin, Qian Wang, Yuqi He, Delin Zhang, Wenping He, Xinyue Ke, Faming Wu, Xiaoshuang Wang

{"title":"Substitutability evaluation and resource utilization of non-medicinal parts of Ardisia crenata Sims, a traditional ethnic medicine.","authors":"Jie Peng, Sha Liu, Fusong Liu, Lin Qin, Qian Wang, Yuqi He, Delin Zhang, Wenping He, Xinyue Ke, Faming Wu, Xiaoshuang Wang","doi":"10.1111/ppl.70068","DOIUrl":"https://doi.org/10.1111/ppl.70068","url":null,"abstract":"The severe scarcity of wild resources of Ardisia crenata Sims (AS) has greatly limited its current clinical and pharmaceutical applications. We aimed to demonstrate the substitutability of stems and leaves for AS roots. In this study, 28 local samples from the Guizhou Province of China were selected for a comprehensive comparison of quality markers, chemical composition and efficacy. HPLC analysis showed that both stems and leaves contained bergenin, the main active ingredient of AS. Metabolomics studies showed that the chemical composition of the three parts was 99% similar. Network pharmacology analysis showed that similar signalling targets including STAT3 and MAPK1, AKT1 and EP300, SRC and TP53, were targeted by three parts. Animal experiments further demonstrated that extracts from the three different parts of the plant relieved the pathological symptoms of acute pharyngitis, and decreased serum inflammatory factors, showing similar efficacy. In sum, these findings strongly proved that stems and leaves can be used as supplements or substitutes for roots, providing a powerful strategy to solve the problem of AS resource shortage and greatly promoting the optimal utilization of AS. The study also anticipated some additional benefits, including the expansion of natural sources of bergenin and the discovery of potential cancer-related targets of AS.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70068"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactive carbonyl species function downstream of reactive oxygen species in chitosan-induced stomatal closure. 在壳聚糖诱导的气孔关闭过程中，活性羰基在活性氧的下游发挥作用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70094

Israt Jahan, Md Moshiul Islam, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Jun'ichi Mano, Yoshiyuki Murata

{"title":"Reactive carbonyl species function downstream of reactive oxygen species in chitosan-induced stomatal closure.","authors":"Israt Jahan, Md Moshiul Islam, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Jun'ichi Mano, Yoshiyuki Murata","doi":"10.1111/ppl.70094","DOIUrl":"10.1111/ppl.70094","url":null,"abstract":"An elicitor, chitosan (CHT), induces stomatal closure in plants, which is accompanied by salicylhydroxamic acid (SHAM)-sensitive peroxidases-mediated reactive oxygen species (ROS) production in guard cells. Reactive carbonyl species (RCS) function downstream of ROS in abscisic acid (ABA) and methyl jasmonate (MeJA) signalling in guard cells. However, the involvement of RCS in CHT-induced stomatal closure is still unknown. In this study, we used transgenic tobacco (Nicotiana tabacum) plants overexpressing Arabidopsis thaliana 2-alkenal reductase (AER-OE tobacco) and Arabidopsis wild-type (WT) plants to investigate whether RCS is involved in CHT-induced stomatal closure. Chitosan-induced stomatal closure was inhibited in the tobacco AER-OE plants. In the WT tobacco and Arabidopsis plants, CHT-induced stomatal closure was inhibited by RCS scavengers, carnosine and pyridoxamine. Chitosan significantly increased RCS production in the WT tobacco and Arabidopsis, but in the tobacco AER-OE plants, chitosan did not increase significantly RCS accumulation. Moreover, neither the application of RCS scavengers to both WT plants nor scavenging RCS by AER-OE affected the CHT-induced ROS accumulation. However, treatment with a peroxidase inhibitor, SHAM, significantly inhibited CHT-induced RCS accumulation in WT tobacco and Arabidopsis plants. Taken together, these results suggest that RCS acts downstream of ROS production in CHT signalling in guard cells of A. thaliana and N. tabacum.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70094"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0