Physiologia plantarum最新文献

Functional Characterisation of Alternative Oxidase Protein Isoproteins in Arabidopsis thaliana. 拟南芥替代氧化酶蛋白同工蛋白的功能特征。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70468

Cunman He, Andreas Hartmann, Minxuan Li, Yanqiao Zhu, Reena Narsai, Kemeng Xiao, Kun Qian, Oliver Berkowitz, Jennifer Selinski, James Whelan

{"title":"Functional Characterisation of Alternative Oxidase Protein Isoproteins in Arabidopsis thaliana.","authors":"Cunman He, Andreas Hartmann, Minxuan Li, Yanqiao Zhu, Reena Narsai, Kemeng Xiao, Kun Qian, Oliver Berkowitz, Jennifer Selinski, James Whelan","doi":"10.1111/ppl.70468","DOIUrl":"https://doi.org/10.1111/ppl.70468","url":null,"abstract":"The Alternative Oxidase (AOX) is encoded by a small gene family in plants. While being one of the most intensively studied plant mitochondrial proteins, it is primarily only one isoform, AOX1a, that is well studied. We investigated the sub-and neo-functionalisation of AOX isoforms in Arabidopsis thaliana by constructing over-expressing lines for all five AOX isoforms in an aox1a knock-out mutant line, where no AOX protein can be detected. In Arabidopsis thaliana, knock-out mutants for aox1a are unable to support germination on antimycin A, despite the presence of four functional AOX genes. Sub-functionalisation was observed for AOX1a in that its 3 kb promoter region supports germination on antimycin A when driving the expression of other AOX isoforms, indicating that it is a promoter-based trait, rather than a specific function of the AOX1a isoprotein. Further evidence of sub-functionalisation was evident as AOX1d and AOX1a tagged with GFP enhanced epidermal expression but not when the other isoforms were used. Arabidopsis AOX1c represents an example of neo-functionalisation as aox1c knock-out had a slightly retarded growth phenotype, while mis-expression of AOX1c, using either the CaMV 35S promoter or the 3 kb of the AOX1c promoter, resulted in enhanced growth. This was even more evident under high-light conditions, where greater tolerance to high light was observed compared to wild type (Col-0) plants. These examples indicate that functional analysis of all AOX isoforms is crucial to gain a full understanding of the role of AOX in plant metabolism and growth.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70468"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12391748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acclimation of Synechocystis sp. PCC 6803 to Alkaline pH Under Ambient Air. 在环境空气条件下，胞囊藻pcc6803对碱性的驯化。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70474

Henna Mustila, Michal Hubáček, Dorota Muth-Pawlak, Yagut Allahverdiyeva

{"title":"Acclimation of Synechocystis sp. PCC 6803 to Alkaline pH Under Ambient Air.","authors":"Henna Mustila, Michal Hubáček, Dorota Muth-Pawlak, Yagut Allahverdiyeva","doi":"10.1111/ppl.70474","DOIUrl":"https://doi.org/10.1111/ppl.70474","url":null,"abstract":"Cyanobacteria can thrive at a wide pH range from neutral up to pH 11 depending on the species. Even though cyanobacteria are alkaliphilic, only limited information on the metabolic acclimation to alkaline pH is available. In this study, we conducted a mass-spectrometry-based comparative proteomic analysis of Synechocystis sp. PCC 6803 grown in BG-11 medium buffered at pH 7.5, pH 8.2, and pH 9.2 under ambient air. When comparing cells grown at pH 8.2 to those at pH 7.5, only minor changes in the proteome were observed, with most of the altered proteins originating from the plasma membrane. However, when comparing cells grown under pH 9.2 to those at pH 7.5, a total of 235 proteins were differentially expressed. Synechocystis cells growing at pH 9.2 seem to display a different composition of bicarbonate transporters. Subunits of the ATP-dependent bicarbonate transporter BCT1 increased in abundance under pH 9.2 compared to pH 7.5 or pH 8.2, while the abundance of the sodium-dependent bicarbonate transporter SbtA was diminished. The increased contribution of the BCT1 complex to total bicarbonate transport at high pH was confirmed by evaluating the activity of different bicarbonate transporters. Furthermore, several changes in the abundance of ion transporters were observed, including upregulation of copper, potassium, and zinc efflux proteins. In contrast, the levels of several proteins involved in nitrogen assimilation and amino acid biosynthesis were reduced at pH 9.2. To conclude, the induction of several transporters indicates the cells' ability to regulate the internal ion and pH homeostasis as well as the carbon: nitrogen ratios under alkaline conditions.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70474"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12382314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SCL15 Regulates the Release of Seed Dormancy in Arabidopsis thaliana by Integrating the Circadian Clock, Hormonal Signals and Cell Wall Remodelling. 通过整合生物钟、激素信号和细胞壁重塑，SCL15调控拟南芥种子休眠释放。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70467

Ming-Jun Gao, Qi Chen, Cathy Coutu, Fuyou Fu, Bianyun Yu, Xiang Li, Z Jeffrey Chen, Dwayne Hegedus

{"title":"SCL15 Regulates the Release of Seed Dormancy in Arabidopsis thaliana by Integrating the Circadian Clock, Hormonal Signals and Cell Wall Remodelling.","authors":"Ming-Jun Gao, Qi Chen, Cathy Coutu, Fuyou Fu, Bianyun Yu, Xiang Li, Z Jeffrey Chen, Dwayne Hegedus","doi":"10.1111/ppl.70467","DOIUrl":"10.1111/ppl.70467","url":null,"abstract":"Dormancy release and germination of the seed are two separate, but continuous phases controlled by both external (e.g., light and temperature) and internal (e.g., circadian clock and hormones) cues. In eudicot seeds, the endosperm tissues play a key role in dormancy release and germination through dynamic modulation of wall components and biomechanics. However, the mode of action by which the circadian oscillator influences dormancy release by modulation of endosperm wall biomechanics remains elusive. SCARECROW-LIKE15 (SCL15) represses embryonic gene expression in seedlings through interaction with HISTONE DEACETYLASE19 (HDA19) in Arabidopsis thaliana. Here, we report that SCL15 plays a positive role in primary dormancy release, which is associated with gene expression changes in circadian, abscisic acid, auxin and cell wall (CW) remodelling pathways, based on studies using SCL15 mutant and Napin promoter-driven SCL15 expression lines. SCL15 was found to affect the expression of genes whose products modify endosperm wall biomechanical features, possibly through regulation of local auxin accumulation and evening-phased clock components. RNA-seq analysis supported the notion that dormancy release is associated with changes in the expression of genes associated with circadian and hormone-mediated pathways, which in turn affect CW structure.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70467"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Speed Breeding of Soybean by Using 22 h Photoperiod Increases Photochemical Efficiency of Pods and Produces Six Generations Per Year. 利用22 h光周期快速育种大豆，可提高豆荚光化学效率，每年可产6代。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70511

Seher Bahar Aciksoz, Shellie Wall, Stuart James Lucas, Mustafa Atilla Yazıcı, Tracy Lawson

{"title":"Speed Breeding of Soybean by Using 22 h Photoperiod Increases Photochemical Efficiency of Pods and Produces Six Generations Per Year.","authors":"Seher Bahar Aciksoz, Shellie Wall, Stuart James Lucas, Mustafa Atilla Yazıcı, Tracy Lawson","doi":"10.1111/ppl.70511","DOIUrl":"https://doi.org/10.1111/ppl.70511","url":null,"abstract":"Fast generation cycling of plants has the potential to overcome the bottleneck of traditional breeding programmes, which often require several years to achieve the desired outcomes. Recent speed breeding methodologies have reduced generation times in both short- and long-day species by optimizing environmental conditions. However, protocols for short-day plants impose a constant short-day photoperiod throughout the entire life cycle, even though plants could benefit from extended light exposure. Here, we report a speed breeding scheme for soybean (Glycine max) based on a long-day photoperiod of 22 h (LD-22 h) applied upon flowering initiation (stage R1) using light-emitting diodes (LEDs) with a cool white (6000 K) and red light (660 nm) spectrum at 550 μmol/(m2s) photosynthetic photon flux at canopy level. We also outline an immature seed germination technique for early harvested green seeds collected from speed-breeding plants that markedly increased the germination rate. Combining these methods allowed our soybean speed breeding system to acquire a 92% germination rate from 58-day-old seeds, enabling six generations y-1 compared to typically only 1-3 using standard approaches. The impact of long photoperiods on soybean leaf and pod photochemical efficiency was examined. Although photosynthetic capacity (Vcmax, Jmax, and Amax) was significantly lower in leaves grown under LD-22 h photoperiod, seed production was unaffected, while PSII operating efficiency (Fq'/Fm') in pods was markedly higher under LD-22 h compared to the SD-10 h photoperiod. Implementing our post-flowering long photoperiod conditions followed by an enhanced germination technique could facilitate rapid breeding for soybeans and be adapted for use with other photoperiod-sensitive short-day crops.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70511"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CO₂-Dependent Promotion of Photosynthesis Drives Metabolic Photoacclimation in Chlamydomonas reinhardtii. 赖因衣藻光合作用的co2依赖性促进代谢性光驯化

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70461

Ana Pfleger, Erwann Arc, Thomas Roach

{"title":"CO2-Dependent Promotion of Photosynthesis Drives Metabolic Photoacclimation in Chlamydomonas reinhardtii.","authors":"Ana Pfleger, Erwann Arc, Thomas Roach","doi":"10.1111/ppl.70461","DOIUrl":"https://doi.org/10.1111/ppl.70461","url":null,"abstract":"Light and inorganic carbon (Ci) drive photosynthesis, which fuels cellular maintenance, energy storage, and growth in photosynthetic organisms. Despite its pivotal role, how primary metabolism adjusts to contrasting light and Ci availability in algae remains elusive. Here, we characterized bioenergetics and profiled primary metabolites of photoautotrophic Chlamydomonas reinhardtii cultures grown under constant low/sub-saturating (LL) or high/saturating (HL) light with 2% (CO2) or ambient 0.04% (Amb) CO2. HL-Amb cells suffered photoinhibition and limitation of photosystem I electron flow at the donor side, but not the acceptor side, indicating use of alternative electron pathways to fuel ATP synthesis. Further, more glycolate was excreted under HL-Amb, indicative of photorespiration. In contrast, HL-CO2 cells upregulated the cytochrome b6f complex, ascorbate metabolism, and PTOX2 for maintaining plastid redox homeostasis. Enhanced glycerol excretion under HL enabled dissipation of excess reducing equivalents to adjust the cellular energy balance. CO2-enhanced photosynthesis promoted respiration and primary metabolite accumulation, driving faster growth while promoting nitrogen (N) metabolism. Hence, Ci-dependent photoacclimation influenced the interplay between the TCA cycle and N assimilation, as supported by proteomic data. Overall, abundant Ci supported growth by promoting electron flow for Ci assimilation, which supplied C skeletons for N assimilation while mitigating photorespiration and photoinhibition.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70461"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological Effect of Thallium in the Facultative Hyperaccumulator Silene latifolia. 铊在兼性超蓄积体麝香中的生理作用。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70469

Gaia Regini, Isabella Bettarini, Ilaria Colzi, Emilio Corti, Alessio Papini, Marco Dainelli, Giorgia Guardigli, Antony van der Ent, Nadia Bazihizina, Cristina Gonnelli

{"title":"Physiological Effect of Thallium in the Facultative Hyperaccumulator Silene latifolia.","authors":"Gaia Regini, Isabella Bettarini, Ilaria Colzi, Emilio Corti, Alessio Papini, Marco Dainelli, Giorgia Guardigli, Antony van der Ent, Nadia Bazihizina, Cristina Gonnelli","doi":"10.1111/ppl.70469","DOIUrl":"https://doi.org/10.1111/ppl.70469","url":null,"abstract":"The metallicolous populations of the facultative Tl hyperaccumulator Silene latifolia are extraordinarily tolerant and capable of accumulating up to 80,000 μg Tl g-1 in nature. A growth stimulatory effect of Tl was observed, and this study set out to determine possible mechanisms. Plants from non-metallicolous and metallicolous populations were subjected to hydroponics dosing experiments at 2.5 and 10 μM Tl. Metal impact on stomatal and non-stomatal photosynthetic constraints, light energy conversion processes and plant anatomy/ultrastructure was assessed over time. Photosynthetic rates improved in 10 μM Tl-treated metallicolous plants by 20% compared to controls, partly due to increased stomatal conductance. The latter was mainly driven by Tl-induced anatomical changes, such as increased central cylinder area and stomatal density, likely to enhance water uptake/translocation and, consequently, leaf metal accumulation. The apparently Tl-favoured CO2 trafficking resulted in ameliorated maximal photosynthetic capacity. The first signs of photosynthetic declines appeared only at very high Tl leaf concentrations (15,000 μg Tl g-1), with limitations involving stomatal and biochemical factors; whereas the photochemical reactions remained functional. The observed Tl-induced stimulatory response in growth and net photosynthetic rate in metallicolous plants shows that Tl improves physiological performance in Silene latifolia, mainly through improved stomatal conductance.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70469"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12382316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drought-Induced Coordination of Photosynthesis, Stomata, and Hydraulics in Maize Leaves Between Sensitive and Tolerant Varieties. 干旱诱导玉米叶片光合、气孔和水力学在敏感和耐旱品种间的协调。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70487

Hao Li, Yanqun Zhang, Kaixuan Du, Xinlong Hu, Yan Mo, Di Xu, Shuji Wang, Baozhong Zhang

{"title":"Drought-Induced Coordination of Photosynthesis, Stomata, and Hydraulics in Maize Leaves Between Sensitive and Tolerant Varieties.","authors":"Hao Li, Yanqun Zhang, Kaixuan Du, Xinlong Hu, Yan Mo, Di Xu, Shuji Wang, Baozhong Zhang","doi":"10.1111/ppl.70487","DOIUrl":"https://doi.org/10.1111/ppl.70487","url":null,"abstract":"Identification of drought-tolerant maize varieties in the context of climate change is critical. Although many studies have reported that the coordination of stomatal and hydraulic conductance of plant leaves ensures the net photosynthetic rate, it is unclear whether the arrangement of these three parameters is consistent among maize varieties differing in drought tolerance. Therefore, in this study, gas exchange parameters, hydraulic properties, and stomatal structure of leaves from eight maize varieties under full and deficit irrigation (DI) were determined. Drought tolerance of varieties was assessed using principal component analysis, and the coordination of photosynthesis, stomatal and hydraulic conductance, as well as stomatal behavior was analyzed between drought-sensitive (DSVs) and drought-tolerant varieties (DTVs). Eight maize varieties were categorized into DSVs and DTVs based on the evaluation of these agronomic and physiological parameters. Significant variety-specific responses of physiological parameters to DI were found, with at least one parameter being significantly affected in each variety. Leaf net photosynthesis rate and stomatal conductance showed a tight coordination with hydraulic conductance among DSVs; however, this coordination was potentially absent among DTVs. Simulations of stomatal behavior based on Ball-Berry and Medlyn models showed that DI significantly reduced the model sensitivity parameters of m and g1 regardless of DSVs and DTVs. The study highlights the importance of physiological trait coordination in drought responses. The coordination between stomatal and hydraulic traits may be absent in DTVs, implying a potentially flexible adaptation strategy that could be exploited to improve maize drought tolerance.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70487"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Lab to Field: Harnessing H₂O₂-Mediated Upregulation of Plant Capacities Under Abiotic Stresses. 从实验室到田间：利用h2o2介导的非生物胁迫下植物能力的上调。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70488

Hassan Iqbal, Chen Yaning, Syed Turab Raza, Sona Karim, Muhammad Shareef, Muhammad Waqas

{"title":"From Lab to Field: Harnessing H2O2-Mediated Upregulation of Plant Capacities Under Abiotic Stresses.","authors":"Hassan Iqbal, Chen Yaning, Syed Turab Raza, Sona Karim, Muhammad Shareef, Muhammad Waqas","doi":"10.1111/ppl.70488","DOIUrl":"https://doi.org/10.1111/ppl.70488","url":null,"abstract":"Climate-driven abiotic stresses, responsible for approximately 50% of global crop yield losses, are putting agriculture under increasing pressure, demanding smarter ways to strengthen plants' natural defenses beyond genetic modification. Hydrogen peroxide (H2O2), long recognized as a key signaling molecule, plays a powerful role in helping plants cope with environmental stress. This review deciphers the mechanistic basis of H2O2-mediated capacity enhancement under diverse stresses (drought, salinity, heavy metals, heat, cold) while also addressing climate-intensified challenges like waterlogging and ultraviolet (UV) radiation. We spotlight its roles in energy partitioning, hormonal signaling, asset optimization, and internal supply chain dynamics, positioning H2O2 as a multifunctional coordinator of stress resilience. Moving beyond the antioxidant narrative, this review highlights the active role of H2O2 in reorganizing plant responses to real-world stress. Can a molecule once known only for causing damage now lead the next wave of environmentally friendly, stress-resilient agriculture? We propose that H2O2-based strategies represent a promising shift toward redox-guided, non-genetic interventions bridging laboratory research with practical field applications and opening new pathways for resilient crop management.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70488"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strigolactone and Hydrogen Sulfide Regulate Carbohydrate Metabolism and Ion Homeostasis Through H⁺-ATPase Activity and K⁺ Retention Under Salt Stress. 盐胁迫下独角麦内酯和硫化氢通过H+- atp酶活性和K+滞留调节碳水化合物代谢和离子稳态。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70475

M Nasir Khan, Manzer H Siddiqui, Francisco J Corpas, Zahid Hameed Siddiqui, Mazen A AlSolami, Khalaf M Alhussaen, Abdulaziz A H Alsumary

{"title":"Strigolactone and Hydrogen Sulfide Regulate Carbohydrate Metabolism and Ion Homeostasis Through H+-ATPase Activity and K+ Retention Under Salt Stress.","authors":"M Nasir Khan, Manzer H Siddiqui, Francisco J Corpas, Zahid Hameed Siddiqui, Mazen A AlSolami, Khalaf M Alhussaen, Abdulaziz A H Alsumary","doi":"10.1111/ppl.70475","DOIUrl":"https://doi.org/10.1111/ppl.70475","url":null,"abstract":"This study investigates the roles of strigolactones (SL) and endogenous hydrogen sulfide (H2S) in regulating physiological processes in tomato seedlings under NaCl-induced stress. Exposure of the seedlings to 100 mM NaCl stress reduced K+ content by 21% while increasing Na+ accumulation by 69%, disrupting the K+/Na+ ratio and impairing H+-ATPase activity. However, the application of SL improved H+-ATPase activity and K+ uptake and reduced Na+ accumulation. However, the application of 1 μM dl-propargylglycine (PAG; an H2S biosynthesis inhibitor) negated these positive effects of SL, suggesting that H2S plays a crucial role in SL-mediated ion homeostasis. NaCl stress also elevated the levels of reactive oxygen species, which were significantly reduced upon SL treatment. On the other hand, the application of PAG reversed these effects, confirming the involvement of H2S in mitigating oxidative stress. Moreover, SL modulated carbohydrate metabolism by promoting starch accumulation and enhancing the activity of key enzymes such as sucrose synthase and soluble acid invertase. This process helps maintain osmoprotection and energy balance under stress conditions. However, these effects were abolished by H2S biosynthesis inhibitor PAG, indicating its critical role in SL-mediated sugar metabolism. Overall, the results indicate that SL mitigates NaCl-induced stress by regulating H+-ATPase activity, maintaining ion homeostasis, reducing oxidative damage, and regulating carbohydrate metabolism via H2S-dependent mechanisms. These findings highlight the potential of SL and H2S to improve plant tolerance to NaCl stress.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70475"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CsMADS Negatively Regulates the Theobromine Content of Leaves in Tea Plants. CsMADS负向调控茶树叶片可可碱含量。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70479

Lingxiao Duan, Hanmo Fang, Jianqiang Ma, Pengcheng Yuan, Jiqiang Jin, Weizhong He, Haoran Liu, Liang Chen

{"title":"CsMADS Negatively Regulates the Theobromine Content of Leaves in Tea Plants.","authors":"Lingxiao Duan, Hanmo Fang, Jianqiang Ma, Pengcheng Yuan, Jiqiang Jin, Weizhong He, Haoran Liu, Liang Chen","doi":"10.1111/ppl.70479","DOIUrl":"https://doi.org/10.1111/ppl.70479","url":null,"abstract":"Theobromine is a significant purine alkaloid found in tea plants, known for its various bioactive functions in humans. While the metabolic pathway for purine alkaloids, particularly involving the methyltransferase CsTCS1, has been largely understood, information regarding the upstream regulatory network of theobromine remains limited. In this study, we collected transcriptome data from tea plants exhibiting different levels of purine alkaloids and applied weighted gene co-expression network analysis (WGCNA) to identify the upstream regulatory network modules associated with each purine alkaloid. The module labeled MEred was discovered as one of the negative regulatory network modules affecting theobromine, with the gene CsMADS identified as a hub gene within this module. To investigate the role of CsMADS in theobromine accumulation, we examined the concentrations of purine alkaloids and the expression patterns of related genes in the CsMADS-silenced tea plants. Results showed that theobromine levels increased in the tender leaves of CsMADS-silenced tea plants, while the expression level of CsTCS1 decreased in these plants. Meanwhile, CsMADS did not influence mechanical harvesting-related traits such as leaf droopiness or leaf tip expansion. In summary, CsMADS negatively regulates the theobromine content in tea leaves without adversely affecting mechanical harvesting capabilities.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70479"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0