Physiologia plantarum最新文献

Regulating Plant Architecture to Enhance the Future of Cereal Crop Production. 调节植物结构，提高未来谷类作物生产。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70367

Nitika Sandhu, Hitashi Aggarwal, Aman Kumar, Gaurav Augustine, Ritika Vishnoi, Ajay Kumar Pandey, Harsh Chauhan, Parveen Chhuneja

{"title":"Regulating Plant Architecture to Enhance the Future of Cereal Crop Production.","authors":"Nitika Sandhu, Hitashi Aggarwal, Aman Kumar, Gaurav Augustine, Ritika Vishnoi, Ajay Kumar Pandey, Harsh Chauhan, Parveen Chhuneja","doi":"10.1111/ppl.70367","DOIUrl":"https://doi.org/10.1111/ppl.70367","url":null,"abstract":"Cereal crops such as rice, wheat, maize, and barley are vital sources of food and income for millions of people worldwide. The architecture of cereal plants, encompassing their height, branching patterns, and inflorescence structure, is crucial in determining their yield potential, adaptability to different environments, and resistance to biotic and abiotic stresses. Boosting cereal production is essential to meet the food demands of a growing population, stimulate economic growth, and ensure sustainable agriculture. Cereal plant architecture is shaped by a combination of genetic, environmental, and hormonal factors. While genetic factors are fundamental in determining plant structure, environmental conditions can influence gene expression, leading to different phenotypic outcomes. Hormones like auxin, cytokinin, and gibberellin regulate key aspects of plant architecture, including root and shoot growth, leaf expansion, and branching. Additionally, brassinosteroids and strigolactone signaling pathways are involved in tiller development in cereal crops. This review aims to provide a comprehensive analysis of the latest research on cereal plant architecture, emphasizing the genetic, environmental, and hormonal regulation. Understanding these factors can support the creation of new cereal varieties with enhanced yield and stress tolerance, contributing to global food security and sustainability amid climate change and population growth.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70367"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zinc Finger Transcriptional Repressor ZOS5-09 Regulates Grain Filling and Development in Rice. 锌指转录抑制因子ZOS5-09调控水稻籽粒灌浆发育

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70376

Priya Jaiswal, Falah Qasim, Arunima Mahto, Ankur Vichitra, Upasana Das, Akhilesh K Tyagi, Pinky Agarwal

{"title":"Zinc Finger Transcriptional Repressor ZOS5-09 Regulates Grain Filling and Development in Rice.","authors":"Priya Jaiswal, Falah Qasim, Arunima Mahto, Ankur Vichitra, Upasana Das, Akhilesh K Tyagi, Pinky Agarwal","doi":"10.1111/ppl.70376","DOIUrl":"https://doi.org/10.1111/ppl.70376","url":null,"abstract":"Grain size is one of the key determinants of grain yield. Our study focuses on a novel seed-preferential C2H2 zinc finger transcription factor, ZOS5-09 (LOC_Os05g38600) that plays an important role in regulating rice grain traits. Rice plants with the ZOS5-09 promoter::GUS construct showed high expression of ZOS5-09 in rice endosperm. In planta reporter effector assays and localization studies showed that ZOS5-09 is a nuclear-localized transcriptional repressor. It has two C2H2 zinc finger domains and a C-terminal NoRS (nucleolar retention signal). Ectopic and seed-preferential overexpression of ZOS5-09 resulted in lethality. Seed-preferential overexpression without NoRS was detrimental to grain filling. Rice plants with knock-down or CRISPR-based knock-out of ZOS5-09 displayed reduced grain length and weight but increased grain width. Grain size change was due to lower cell proliferation and increased cell size in the transverse direction because of downregulation of cell cycle-related genes and increased expression of expansins. Decreased expression of ZOS5-09 also resulted in reduced total starch and protein content and higher endosperm chalkiness, thus negatively affecting grain quality. ZOS5-09 directly bound to a zinc finger-binding site and regulated a seed storage protein-encoding gene, GLU6. It acted as a repressor by promoting deacetylation upon interaction with a histone deacetylase. In summary, our results indicate that an optimum expression of ZOS5-09 is essential for proper rice grain development. Our study highlights the role of a transcriptional repressor in regulating rice grain traits and improves our understanding of the transcriptional regulatory networks affecting grain size.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70376"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Photoregulatory Mechanisms of Capsaicinoids Biosynthesis and Accumulation of Capsaicinoids in Capsicum annuum In Vitro Cultures. 辣椒素体外合成及积累的光调控机制研究

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70370

Monisha Arya, Gyanendra Kumar, Parvatam Giridhar

{"title":"Unraveling the Photoregulatory Mechanisms of Capsaicinoids Biosynthesis and Accumulation of Capsaicinoids in Capsicum annuum In Vitro Cultures.","authors":"Monisha Arya, Gyanendra Kumar, Parvatam Giridhar","doi":"10.1111/ppl.70370","DOIUrl":"https://doi.org/10.1111/ppl.70370","url":null,"abstract":"Light is one of the essential environmental factors that significantly influences the biosynthesis of secondary metabolites in plants. To explicate their effect, exposure of monochromatic LED light spectra (yellow, blue, green, red, and white) on the accumulation of capsaicinoids, phenolic compounds, antioxidant capacity, and regulation of transcriptional changes in capsaicinoids biosynthesis genes at 24, 48, and 72 h in chili callus cultures was investigated. Blue light significantly increased capsaicin and dihydrocapsaicin contents by 41.43-fold and 4.36-fold, respectively, compared to white light. Pearson correlation analysis confirmed a strong positive correlation between total phenolics, total flavonoids, and antioxidant capacity (r > 0.85). Gene expression analysis revealed that the biosynthetic genes (pAMT, KAS, ACS, ACL, CL, C4H, and CS) were upregulated, and their associated transcription factors-MYB, ERF, and JERF-showed varied expression, indicating their putative regulatory role in capsaicinoid biosynthesis under different monochromatic LED conditions. Our findings demonstrate that the targeted monochromatic LED lights can enhance bioactive compound production in C. annuum callus cultures, providing a favorable strategy for feasible production of the industrially important compound \"capsaicin\" on a large scale for pharmaceutical and food applications.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70370"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Use of Flocculation as a Preconcentration Step in the Microalgae Harvesting Process. 絮凝预处理在微藻收获过程中的应用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70366

Zivan Gojkovic, Aleksandra Skrobonja, Vuk Radojicic, Benedetta Mattei

{"title":"The Use of Flocculation as a Preconcentration Step in the Microalgae Harvesting Process.","authors":"Zivan Gojkovic, Aleksandra Skrobonja, Vuk Radojicic, Benedetta Mattei","doi":"10.1111/ppl.70366","DOIUrl":"10.1111/ppl.70366","url":null,"abstract":"Flocculation is a widely utilized separation technique in colloid chemistry, the chemical industry, and wastewater treatment. However, its application in microalgal biomass production and downstream processing remains limited. Biomass harvesting represents a significant cost factor in large-scale microalgae production, with the separation of biomass from the liquid medium accounting for up to one-third of total production expenses. The use of flocculants as a pre-treatment step has emerged as a promising approach to reducing these costs. Laboratory-scale flocculation is commonly employed to optimize flocculant dosage and assess strain-specific flocculation efficiencies. Meanwhile, pilot-scale flocculation, when applied before centrifugation, provides critical insights into the applicability of specific flocculant-strain combinations and their impact on yield and biomass quality. Numerous studies have investigated the flocculation behavior of various microalgal strains using a wide range of chemically distinct flocculants at the laboratory scale. This review highlights recent advancements in microalgal flocculation as a pre-centrifugation strategy and outlines future perspectives for achieving cost-effective, large-scale microalgal biomass production as a globally viable resource.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70366"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological, Physiological, and Biochemical Impacts of Drought on Wheat-Pest-Pathogen Interactions. 干旱对小麦-病虫害相互作用的形态、生理和生化影响。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70364

Maroa Mohammed Al-Aisaee, Rethinasamy Velazhahan, Ahmad Nawaz, Muhammad Farooq

{"title":"Morphological, Physiological, and Biochemical Impacts of Drought on Wheat-Pest-Pathogen Interactions.","authors":"Maroa Mohammed Al-Aisaee, Rethinasamy Velazhahan, Ahmad Nawaz, Muhammad Farooq","doi":"10.1111/ppl.70364","DOIUrl":"https://doi.org/10.1111/ppl.70364","url":null,"abstract":"Plants often face a combination of abiotic and biotic stresses, such as drought, disease, and insect infestation. However, the interactions among these stressors remain poorly understood. This study investigates the effects of combined drought and biotic stress, particularly leaf rust (Puccinia triticina) and aphid (Rhopalosiphum maidis) infestations, on the wheat genotype TW1509. Two experiments were conducted under three water regimes: well-watered (WW; 80% WHC), moderate drought (MD; 60% WHC), and severe drought (SD; 40% WHC). MD intensified aphid and rust infestations, likely due to increased plant vulnerability. Aphid stress led to irregular stomatal patterns, reduced chlorophyll, and impaired photosynthesis. Under combined drought-aphid stress, significant increases were observed in stomatal density, electrolyte leakage, flavonoid content, and proline accumulation across all drought levels, along with reduced photosynthetic activity. Similarly, drought-rust stress elevated flavonoids, catalase activity, and proline content, though these were linked to decreased morphological traits. These findings highlight the complexity of wheat responses to concurrent stresses. The findings suggest that drought severity modulates plant susceptibility to pests and pathogens by influencing physiological resilience, nutrient dynamics, and defense responses. This study highlights the necessity of integrating multi-stress considerations into wheat management strategies to ensure sustainable productivity. A deeper understanding of these stress interactions is crucial for developing targeted interventions to mitigate their adverse effects on wheat.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70364"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Functional Analysis of the Fragrance Terpene Synthase Gene PpTPS5 in Pyrethrum parthenium. 除虫菊香精萜合成酶基因PpTPS5的鉴定与功能分析。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70333

Fang Wang, Daihao Li, Sixian Zeng, Xueqian Gao, Guilan Wu, Di Huang, Zihan Cao, Qin Wang, Rangcai Yu, Yanping Fan

{"title":"Identification and Functional Analysis of the Fragrance Terpene Synthase Gene PpTPS5 in Pyrethrum parthenium.","authors":"Fang Wang, Daihao Li, Sixian Zeng, Xueqian Gao, Guilan Wu, Di Huang, Zihan Cao, Qin Wang, Rangcai Yu, Yanping Fan","doi":"10.1111/ppl.70333","DOIUrl":"https://doi.org/10.1111/ppl.70333","url":null,"abstract":"Pyrethrum parthenium, a perennial herb from the genus Pyrethrum within the Asteraceae family, is known for its potent fragrance and significant economic value. Nevertheless, the mechanisms involved in the synthesis and regulation of its floral fragrance compounds remain unclear. This study used gas chromatography-mass spectrometry (GC-MS) to analyze the volatile compounds in five parts of P. parthenium: tubular flowers, ligulate flowers, sepals, receptacles, and leaves. The results showed that the volatile aromatic components include terpenes such as camphor, α-pinene, camphene, d-limonene, β-caryophyllene, and β-farnesene, with camphor being the main volatile compound. Based on the transcriptome data of tubular flowers of P. parthenium, a terpene synthase gene, PpTPS5, was identified. The results of both in vitro and in vivo enzymatic assays demonstrated that PpTPS5 functions as a bifunctional terpene synthase gene. The qRT-PCR results showed that the gene expression pattern of PpTPS5 is correlated with the release pattern of the corresponding terpene aromatic compounds in P. parthenium. Subcellular localization and inhibitor experiments indicated that PpTPS5 functions in the cytosol. Additionally, dual-luciferase and electrophoretic mobility shift assay (EMSA) assays revealed that PpMYB9 can bind to the promoter of PpTPS5 to regulate the biosynthesis of terpenoids. In conclusion, the results of this study provide a theoretical basis for further exploration of the transcriptional regulation of terpene floral fragrance compounds in P. parthenium.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70333"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Belowground Interactions in a Barley Cultivar Mixture: Root Distribution and Arbuscular Mycorrhizal Contributions to Uptake of Heterogeneous Phosphorus. 大麦混合品种地下相互作用：根分布和丛枝菌根对异质磷吸收的贡献。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70363

Lorène Siegwart, Dorette Müller-Stöver, Dorte Bodin Dresbøll, John Larsen, Tino Colombi, Thomas Keller, Frederik van der Bom

{"title":"Belowground Interactions in a Barley Cultivar Mixture: Root Distribution and Arbuscular Mycorrhizal Contributions to Uptake of Heterogeneous Phosphorus.","authors":"Lorène Siegwart, Dorette Müller-Stöver, Dorte Bodin Dresbøll, John Larsen, Tino Colombi, Thomas Keller, Frederik van der Bom","doi":"10.1111/ppl.70363","DOIUrl":"https://doi.org/10.1111/ppl.70363","url":null,"abstract":"Cultivar mixtures have the potential to mitigate abiotic stress and stabilize crop yields, but their belowground dynamics remain poorly understood. We evaluated phosphorus (P) uptake by two contrasting spring barley (Hordeum vulgare L.) cultivars (\"Anneli\" and \"Feedway\"), grown either in 50:50 mixture or as pure stand. The cultivars were grown in mesocosms under four P fertilization treatments: low-P, homogeneous high-P (90 mg P/kg), and localized P hotspots (100 mg P) placed either in the topsoil (5 cm) or subsoil (35 cm). To trace P uptake pathways, the hotspots were labeled with 33P and enclosed in mesh bags allowing only mycorrhizal hyphae (25 μm) or both roots and hyphae (2 mm) to access the hotspot. After 35 days, we measured aboveground biomass, total P content, 33P specific activity, and root biomass, length, diameter, and arbuscular mycorrhiza fungi (AMF) root colonization. In the mixture, reduced P uptake by \"Feedway\" led to lower overall performance compared to pure stand. Root modifications in the mixture did not enhance biomass or P acquisition, potentially due to decreased AMF colonization. Although different P placements altered P uptake patterns, they did not increase total P uptake. Roots accessed the P hotspots and acquired 33P without notable proliferation in the enriched zones. Our findings underscore the complexity of belowground interactions involving root distribution, competition for P, and AMF, and highlight the need for future research to optimize nutrient acquisition and performance in cultivar mixtures.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70363"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alleviating Soybean Salt Stress via Suaeda salsa Intercropping: Roles of Desalinization and Root Interactions. 盐田间作缓解大豆盐胁迫：脱盐和根系互作的作用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70358

Shiqi Wang, Jinbiao Liu, Jiliang Zheng, Yalan Liu, Changyan Tian

{"title":"Alleviating Soybean Salt Stress via Suaeda salsa Intercropping: Roles of Desalinization and Root Interactions.","authors":"Shiqi Wang, Jinbiao Liu, Jiliang Zheng, Yalan Liu, Changyan Tian","doi":"10.1111/ppl.70358","DOIUrl":"https://doi.org/10.1111/ppl.70358","url":null,"abstract":"Halophyte-based intercropping alleviates salt stress in glycophytes by desalinization. However, the role of root interactions, which are key to system sustainability, is often overlooked. This study evaluated soybean (Glycine max) salt tolerance when intercropped with Suaeda salsa, a halophyte with high salt tolerance, under different root interaction modes: plastic sheet separation (PL), nylon mesh separation (NL), and no separation (NS). Soil electrical conductivity did not differ significantly between NL and PL, indicating that soybean salt tolerance differences arose from root interactions, while the differences between NS and PL resulted from both root interactions and desalinization. Results showed that desalinization significantly reduced Na+ and Cl- content in both soil and soybean shoots in saline soils. However, it exacerbated reactive oxygen species (ROS) levels and introduced competition for soluble nutrients, partially counteracting its positive effects on biomass. Root interactions significantly increased soybean biomass by 80% without a significant effect on Na+ and Cl- content, but effectively scavenged salt stress-induced ROS through the upregulation of antioxidant enzymes (glutathione peroxidase and glutathione reductase) and non-enzymatic antioxidants (glutathione, melatonin, flavonoids), and alleviated desalinization-induced oxidative damage by further enhancing guaiacol peroxidase and ascorbate peroxidase activities. These results highlight the positive role of root interactions in alleviating soybean salt stress through enhanced antioxidant capacity. Additionally, root interactions demonstrate the capacity to enhance nutrient uptake in soybean such as Ca and Mg. Our findings suggest that, with water and fertilizer management, Suaeda salsa-soybean intercropping can be sustainably cultivated in saline soils.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70358"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MpANT-Auxin Loop Modulates Marchantia polymorpha Development. mpant -生长素环调控多形地豆发育。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70365

Dipp-Álvarez Melissa, Lorenzo-Manzanarez J Luis, Flores-Sandoval Eduardo, Espinal-Centeno Annie, Méndez-Álvarez Domingo, Fisher J Tom, León-Ruiz Jesús, Olvera-Martínez Fernando, Bowman L John, Arteaga-Vázquez A Mario, Alfredo Cruz-Ramírez

{"title":"The MpANT-Auxin Loop Modulates Marchantia polymorpha Development.","authors":"Dipp-Álvarez Melissa, Lorenzo-Manzanarez J Luis, Flores-Sandoval Eduardo, Espinal-Centeno Annie, Méndez-Álvarez Domingo, Fisher J Tom, León-Ruiz Jesús, Olvera-Martínez Fernando, Bowman L John, Arteaga-Vázquez A Mario, Alfredo Cruz-Ramírez","doi":"10.1111/ppl.70365","DOIUrl":"https://doi.org/10.1111/ppl.70365","url":null,"abstract":"AINTEGUMENTA-LIKE/PLETHORA/BABYBOOM (APB) genes are considered part of the ancestral developmental toolkit in land plants. In Arabidopsis thaliana, these transcription factors are induced by auxin and are primarily expressed in tissues with actively dividing cells, where they play essential roles in organ development. Marchantia polymorpha, a liverwort that diverged from A. thaliana early in embryophyte evolution, possesses a single APB ortholog, MpAINTEGUMENTA (MpANT), encoded in its genome. In this study, we aimed to characterize the function of MpANT. Analysis of a transcriptional fusion line indicates that MpANT is predominantly expressed in the meristematic region. We report that the MpANT promoter region contains several cis-acting Auxin Responsive Elements (AREs) and demonstrate that its expression, which occurs predominantly in meristematic regions, is significantly altered by the addition of exogenous auxin and inhibition of auxin transport. These findings indicate that MpANT acts downstream of Auxin Response Factors (ARFs) and auxin signaling. Analyses of loss- and gain-of-function MpANT alleles highlight the importance of this transcription factor in meristem maintenance and cell proliferation. Additionally, we found that MpANT acts upstream of the auxin transporter MpPIN1 by influencing auxin distribution. Taken together, our findings reveal a feedforward regulatory loop involving auxin, MpANT, and MpPIN1 that is important for Marchantia development.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70365"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Do guard cells have single or multiple defense mechanisms in response to flg22? 保卫细胞对flg22是否有单一或多重防御机制？

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-05-01 DOI: 10.1111/ppl.70249

Zalán Czékus, András Kukri, Atina Martics, Boglárka Pollák, Árpád Molnár, Attila Ördög, Györgyi Váradi, László Galgóczy, Rebeka Papp, Liliána Tóth, Katalin Ágnes Kocsis, Nóra Faragó, Nikolett Bódi, Mária Bagyánszki, Gabriella Szalai, Kamirán Áron Hamow, Péter Poór

{"title":"Do guard cells have single or multiple defense mechanisms in response to flg22?","authors":"Zalán Czékus, András Kukri, Atina Martics, Boglárka Pollák, Árpád Molnár, Attila Ördög, Györgyi Váradi, László Galgóczy, Rebeka Papp, Liliána Tóth, Katalin Ágnes Kocsis, Nóra Faragó, Nikolett Bódi, Mária Bagyánszki, Gabriella Szalai, Kamirán Áron Hamow, Péter Poór","doi":"10.1111/ppl.70249","DOIUrl":"https://doi.org/10.1111/ppl.70249","url":null,"abstract":"Bacterial flagellin (flg22) induces rapid and permanent stomatal closure. However, its local and systemic as well as tissue- and cell-specific effects are less understood. Our results show that flg22 induced local and systemic stomatal closure in intact tomato plants, which was regulated by reactive oxygen- and nitrogen species, and also affected the photosynthetic activity of guard cells but not of mesophyll cells. Interestingly, rapid and extensive local expression of Ethylene response factor 1 was observed after exposure to flg22, whereas the relative transcript levels of Defensin increased only after six hours, especially in systemic leaves. Following local and systemic ethylene emission already after one and six hours, jasmonic acid levels increased in the local leaves after six hours of flg22 treatment. Using immunohistochemical methods, significant defensin accumulation was found in the epidermis and stomata of flg22-treated leaves and above them. Immunogold labelling revealed significant levels of defensins in the cell wall of the mesophyll parenchyma and guard cells. Furthermore, single cell qRT-PCR confirmed that guard cells are able to synthesise defensins. It can be concluded that guard cells are not only involved in the first line of plant defense by regulating stomatal pore size, but can also defend themselves and the plant by producing and accumulating antimicrobial defensins where phytopathogens can penetrate.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70249"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0