Physiologia plantarum最新文献

Transgenic Cynodon dactylon overexpressing CdPIF4 alters plant development and cold stress tolerance. 过表达CdPIF4的转基因Cynodon dactylon改变了植物的发育和对冷胁迫的耐受性。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70025

Xiao Xu, Xiaoyan Liu, Yanling Yin, Shugao Fan, Yunjie Qi, Yiquan Xing, Jinmin Fu

{"title":"Transgenic Cynodon dactylon overexpressing CdPIF4 alters plant development and cold stress tolerance.","authors":"Xiao Xu, Xiaoyan Liu, Yanling Yin, Shugao Fan, Yunjie Qi, Yiquan Xing, Jinmin Fu","doi":"10.1111/ppl.70025","DOIUrl":"https://doi.org/10.1111/ppl.70025","url":null,"abstract":"Bermudagrass [Cynodon dactylon (L.) Pers.] is widely used for soil remediation, livestock forage, and as turfgrass for sports fields, parks, and gardens due to its resilience and adaptability. However, low temperatures are critical factors limiting its geographical distribution and ornamental season, even preventing its safe overwintering. PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) acts as a hub transcription factor, not only regulating various light responses but also integrating multiple external stimuli to improve plant productivity and architectural adaptation under adverse stress conditions, which makes it potential as a target gene. In this study, we cloned and characterized the CdPIF4 genes in bermudagrass. Expression analysis revealed that it is predominantly expressed in leaves and is regulated by photoperiod and cold stress. Using Agrobacterium-mediated genetic modification, we successfully generated CdPIF4a-overexpressing bermudagrass lines. Under cold stress at 4°C, these transgenic plants demonstrated enhanced cold tolerance, as indicated by higher relative water content, reduced membrane damage, and lower levels of lipid peroxidation levels. Photosynthetic analysis revealed that CdPIF4a-overexpressing plants exhibited higher light energy capture and transfer efficiency at this low temperature, with less energy loss. Additionally, they showed higher antioxidant enzyme activity and lower levels of reactive oxygen species levels. The responsive regulation of cold stress-related genes further validated the role of the CdPIF4a gene in enhancing cold tolerance. This study elucidates that CdPIF4 enhances cold tolerance in bermudagrass through physiological and molecular mechanisms, offering new insights and valuable genetic resources for advancing cold resistance research in bermudagrass and other grass species.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70025"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The molecular mechanism of transforming red light signal to (E)-β-caryophyllene biosynthesis in Arabidopsis. 拟南芥将红光信号转化为(E)-β-石竹烯生物合成的分子机制

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70065

Chuanjia Xu, Xin Wang, Malakkhanim Mehraliyeva, Jia Sun, Fangfang Chen, Changfu Li, Zhengqin Xu, Nan Tang, Yansheng Zhang

引用次数: 0

A NAC transcription factor NAC50 regulates Fe reutilization in Arabidopsis under Fe-deficient condition.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70047

Jing Huang, Chun Yan Tu, Hao Yu Wang, Qiang Zhang, Ren Fang Shen, Lu Zheng, Xiao Fang Zhu

{"title":"A NAC transcription factor NAC50 regulates Fe reutilization in Arabidopsis under Fe-deficient condition.","authors":"Jing Huang, Chun Yan Tu, Hao Yu Wang, Qiang Zhang, Ren Fang Shen, Lu Zheng, Xiao Fang Zhu","doi":"10.1111/ppl.70047","DOIUrl":"https://doi.org/10.1111/ppl.70047","url":null,"abstract":"A lack of iron (Fe) inhibits the growth and development of plants, leading to reduced agricultural yields and quality. In the last ten years, numerous studies have focused on the induction of Fe uptake and translocation under Fe deficiency, but the regulatory mechanisms governing Fe reutilization within plants are still not well understood. Here, we demonstrated the involvement of the NAM/ATAF1/2/CUC2 (NAC) transcription factor NAC50 in response to Fe shortage. The content of soluble Fe was greatly reduced in nac50 mutants, leading to increased chlorosis in the newly emerging leaves under the Fe-deficient condition. Subsequent investigation revealed that the cell wall of the nac50 mutants' roots accumulated more Fe, along with an increment in hemicellulose content, indicating that a cell wall-associated Fe reutilization pathway was involved in the NAC50-regulated Fe insufficiency response. Interestingly, the expression of NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3), a key enzyme in the abscisic acid (ABA) biosynthetic pathway, was down-regulated in the Fe-deficient nac50 mutants, resulting in decreased endogenous ABA level and Fe-deficient sensitive phenotype. Since no direct relationship was observed between NAC50 and NCED3, this suggests a potential role of NAC50 in mediating the ABA accumulation. Moreover, exogenous ABA application in the nac50 mutant restored Fe deficiency resistance to the level observed in wild-type plants (WT), indicating that NAC50 induced the cell wall Fe reutilization potentially through the regulation of ABA accumulation.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70047"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plants as biofactories for production of the aphid sex pheromone nepetalactone.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70110

Abraham Ontiveros-Cisneros, Jule Salfeld, Bao-Jian Ding, Hong-Lei Wang, Oliver Moss, Magne Friberg, Alex Van Moerkercke, Christer Löfstedt, Olivier Van Aken

{"title":"Plants as biofactories for production of the aphid sex pheromone nepetalactone.","authors":"Abraham Ontiveros-Cisneros, Jule Salfeld, Bao-Jian Ding, Hong-Lei Wang, Oliver Moss, Magne Friberg, Alex Van Moerkercke, Christer Löfstedt, Olivier Van Aken","doi":"10.1111/ppl.70110","DOIUrl":"10.1111/ppl.70110","url":null,"abstract":"Aphids cause massive agricultural losses through direct damage or as pathogen vectors. Control often relies on insecticides, which are expensive and not selective. An interesting alternative is to use aphid sex pheromones nepetalactone (NON) and nepetalactol (NOL) to interfere with aphid mating or attract aphid predators. Here, we explore production of these compounds in plants, as their precursors can be derived from mevalonate (MVA) and methylerythritol phosphate (MEP) pathways. By introducing six genes, including a major latex protein-like (MLPL) enzyme, we engineered a functional nepetalactol biosynthetic pathway into plants. Transient expression of these enzymes in N. benthamiana caused production of nepetalactone, without the need for external supplementation with substrates. Targeting all six enzymes into the chloroplast appeared to result in higher NON yields than just chloroplast-targeting the first two enzymes. We could not detect NOL, suggesting it is rapidly oxidised to NON. In addition, we produced NON in stably transformed Camelina sativa (Camelina) lines. Interestingly, the specific NON enantiomer was different in N. benthamiana compared to in Camelina, indicating the value of different platforms for producing specific isoforms. This opens possibilities for using plants as green factories of pheromones for baits or as pheromone dispensers that interfere with insect behaviour.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70110"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytohormones enhance resistance to Tenebrio molitor by regulating reactive oxygen species and phenolic metabolism in pigeon pea. 植物激素通过调节鸽子豆中的活性氧和酚代谢增强其对 Tenebrio molitor 的抗性。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70111

Jie Yang, Hongquan Li, Su Zhang, Yuexin Zhang, Jianbo Xie, Michael Wink, Yujie Fu

{"title":"Phytohormones enhance resistance to Tenebrio molitor by regulating reactive oxygen species and phenolic metabolism in pigeon pea.","authors":"Jie Yang, Hongquan Li, Su Zhang, Yuexin Zhang, Jianbo Xie, Michael Wink, Yujie Fu","doi":"10.1111/ppl.70111","DOIUrl":"https://doi.org/10.1111/ppl.70111","url":null,"abstract":"Pigeon pea is an important economic crop with medicinal and nutritional value. Unfortunately, pest infestation of leaves during postharvest storage seriously affects the quality of pigeon pea. Phytohormones play a crucial role in disease and pest defence by regulating the accumulation of specialized metabolites. Still, their impact on the postharvest storage of pigeon pea has not been reported. In this study, the physiological parameters and main phenotypes of pigeon pea leaves treated with MeJA, ABA, and GA were investigated for the first time. The activity of the antioxidant enzyme system, which eliminates reactive oxygen species, was enhanced by applying MeJA, GA, and ABA. MeJA, GA, and ABA significantly affected crown width, plant height, and relative water content in pigeon pea, respectively. Metabolomic profiling analysis identified phenolic compounds as the main differentially accumulated metabolites (DAMs). UPLC-QqQ-MS/MS identified stilbenes, flavanones, flavones, isoflavones and anthocyanins as major phenolic compounds responsive to MeJA, GA, and ABA induction. By feeding insects, it was found that the insects fed on MeJA-, ABA-, and GA-treated leaves less than on control leaves. Correlation analysis confirmed that isoflavones play an important role in this process. Moreover, the expression of key genes involved in flavonoid biosynthetic pathways and anti-insect-related genes was regulated by MeJA, GA, and ABA. Overall, this work provides a new strategy for the cultivation and storage of pigeon pea or other commercial crops and preliminarily clarifies that flavonoid metabolites under plant hormone treatment can promote plant growth and defence against insects by regulating reactive oxygen species.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70111"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity and population structure analysis of short-day onions using molecular markers in association with resistance to Fusarium basal rot. 短日洋葱抗枯萎病遗传多样性及群体结构分析。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70042

SaeidReza Poursakhi, Hossein Ali Asadi-Gharneh, Mehdi Nasr-Esfahani, Zahra Abbasi, Hamed Hassanzadeh Khankahdani

{"title":"Genetic diversity and population structure analysis of short-day onions using molecular markers in association with resistance to Fusarium basal rot.","authors":"SaeidReza Poursakhi, Hossein Ali Asadi-Gharneh, Mehdi Nasr-Esfahani, Zahra Abbasi, Hamed Hassanzadeh Khankahdani","doi":"10.1111/ppl.70042","DOIUrl":"https://doi.org/10.1111/ppl.70042","url":null,"abstract":"In this research, we analyzed Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Sequence-related amplified polymorphism (SRAP) markers to evaluate the genetic diversity of eighteen different onion genotypes with various resistant levels to FOC. The results showed that the polymorphism means between RAPD primers was 61.11 to 81.81%; ISSR primers, 62.50 to 81.81%; and SRAP primers, 56.25 to 76.25%. Overall, by assessing MI, PIC, I and H indices, indicating the best thrive in evaluating the genetic diversity of the related onion populations. There is a significant correlation between the generated dendrograms based on similarity matrices. The classification pattern in dendrograms shows a corresponding correlation with the FOC disease severity bunches. So in all three markers studied, 'Saba' and 'Saba - HS', the most resistant ones to FOC disease, were grouped in a branch, and the 'Sahar - HS' and 'Golden Eye', the most susceptible ones were also grouped in another branch separately. This finding indicates that predominant primers act as markers linked to resistance gene(s) against FOC, which can be used to select onions resistant to FOC disease in any breeding scheme.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70042"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary dynamics of nitrate uptake, assimilation, and signalling in plants: adapting to a changing environment. 植物对硝酸盐吸收、同化和信号传导的进化动力学：适应不断变化的环境。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70069

Xiaojia Zhang, Shufeng Bai, Hui Min, Yuxuan Cui, Yibo Sun, Yulong Feng

{"title":"Evolutionary dynamics of nitrate uptake, assimilation, and signalling in plants: adapting to a changing environment.","authors":"Xiaojia Zhang, Shufeng Bai, Hui Min, Yuxuan Cui, Yibo Sun, Yulong Feng","doi":"10.1111/ppl.70069","DOIUrl":"https://doi.org/10.1111/ppl.70069","url":null,"abstract":"Nitrogen (N) is a crucial macronutrient for plant growth, with nitrate as a primary inorganic N source for most plants. Beyond its role as a nutrient, nitrate also functions as a signalling molecule, influencing plant morphogenetic development. While nitrate utilization and signalling mechanisms have been extensively studied in model plants, the origin, evolution, and diversification of core components in nitrate uptake, assimilation, and signalling remain largely unexplored. In our investigation, we discovered that deep sea algae living in low nitrate conditions developed a high-affinity transport system (HATS) for nitrate uptake and a pathway of nitrate primary assimilation (NR-NiR-GS-GOGAT). In contrast, low-affinity transport systems (LATS) and the plastid GS originated from the ancestors of land and seed plants, respectively. These adaptations facilitated amino acid acquisition as plants conquered terrestrial environments. Furthermore, the intricate nitrate signalling, relying on NRT1.1 and NLP7, evolved stepwise, potentially establishing systematic regulation in bryophytes for self-regulation under complex terrestrial nitrate environments. As plants underwent terrestrialization, they underwent adaptive changes to thrive in dynamic nitrate environments, continually enhancing their nitrate uptake, assimilation, and signal transduction abilities.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70069"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of stored carbon for new organ development in apple saplings in early spring for two consecutive years after ¹³C labelling. 13C标记后连续两年苹果幼树早春贮藏碳对新器官发育的影响。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70077

Shogo Imada, Yasuhiro Tako

{"title":"Use of stored carbon for new organ development in apple saplings in early spring for two consecutive years after 13C labelling.","authors":"Shogo Imada, Yasuhiro Tako","doi":"10.1111/ppl.70077","DOIUrl":"https://doi.org/10.1111/ppl.70077","url":null,"abstract":"The use of stored carbon is essential for new organ development in deciduous trees during early spring. However, the contribution of carbon to the development of new organs in early spring of subsequent years is not well understood. Using a 13C labelling approach, we investigated the reallocation of assimilated carbon into new aboveground organs on apple (Malus domestica) saplings in the following two years. Eight three-year-old potted saplings were exposed to 13CO2 in an exposure chamber on each of eight different dates during the growth season. Some of the trees were harvested in the late autumn of the same year. The remaining trees were transferred to a field and cultivated during the two following growing seasons. We directly showed that the assimilated 13C was used to develop terminal and flower buds for two consecutive years after labelling. The proportions of the concentration of 13C remobilized to the terminal and flower buds in the second year were 5 and 24% of those in the first year after labelling, respectively. The concentration of assimilated 13C was higher in the terminal buds than in the flower buds in the first year after the labelling, while opposite results were found in the second year. This study demonstrates that the stored carbon used for the development of new organs was a mixture of recent- and old-stored carbon and indicates that recently-stored carbon was preferentially used to develop new organs. We also indicated that the stored carbon was remobilized to flower buds during development.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70077"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The spatiotemporal changes of metabolites in Pinellia ternata at different development stages by MALDI-MSI. 利用MALDI-MSI研究半夏不同发育阶段代谢物的时空变化。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70049

Yifei Pei, Xue Feng, Ziyi Liu, Jialei Chen, Jialu Wang, Xiangyu Zhang, Haitao Liu, Xiwen Li

{"title":"The spatiotemporal changes of metabolites in Pinellia ternata at different development stages by MALDI-MSI.","authors":"Yifei Pei, Xue Feng, Ziyi Liu, Jialei Chen, Jialu Wang, Xiangyu Zhang, Haitao Liu, Xiwen Li","doi":"10.1111/ppl.70049","DOIUrl":"https://doi.org/10.1111/ppl.70049","url":null,"abstract":"Pinellia ternata is an herb species in the Pinellia genus with significant economic value due to its medicinal properties. Understanding the accumulation and spatial distribution characteristics of metabolites during the development of the medicinal part, the rhizome of P. ternata (PR), provides a basis for targeted metabolic regulation and quality evaluation. In this study, we used matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI) and MS/MS to analyse metabolites at 5 representative stages (S1 to S5) of rhizome development in cross and longitudinal sections of the rhizome. A total of 168 metabolites were detected, with 13 being metabolites previously reported in PR. Additionally, Venn analysis revealed 12 bioactive differential metabolites during the growth process. Their spatial distribution and composition were analyzed, showing that alkaloids and amino acids were significantly distributed throughout the entire region and had higher relative contents compared to other metabolites. Flavonoids were more distributed in the outer regions of PR, potentially playing a greater role in combating biotic or abiotic stresses. Specifically, in cross-sections, arginine, nicotinamide, and 2-pentylpyridine showed a clear trend of accumulation from the outer to the inner from S1 to S5, while trigonelline, adenosine, cytidine, 3,4-dihydroxycinnamyl alcohol, raffinose, choline alfoscerate, liquiritin, and apii exhibited the opposite trend. For longitudinal sections, trigonelline, 2-pentylpyridine, choline alfoscerate and baicalein showed a trend of accumulation from the area of bud end to the far region during S1 to S5, while arginine showed opposite distribution trends. These findings deepen our understanding of the metabolic processes involved in the development of PR and have potential implications for variety improvement and quality control.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70049"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AsGAD1 cloned from creeping bentgrass modulates cadmium tolerance of Arabidopsis thaliana by remodelling membrane lipids and cadmium uptake, transport and chelation.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70063

Yilin Di, Yiqin Cao, Dandan Peng, Ying Liu, Zhou Li

{"title":"AsGAD1 cloned from creeping bentgrass modulates cadmium tolerance of Arabidopsis thaliana by remodelling membrane lipids and cadmium uptake, transport and chelation.","authors":"Yilin Di, Yiqin Cao, Dandan Peng, Ying Liu, Zhou Li","doi":"10.1111/ppl.70063","DOIUrl":"https://doi.org/10.1111/ppl.70063","url":null,"abstract":"The gene GAD1 encodes a glutamate decarboxylase, which is a rate-limiting enzyme for the biosynthesis of endogenous γ-aminobutyrate acid (GABA), but a potential role of GAD1 in regulating cadmium (Cd) tolerance needs to be further elucidated in plants. The objective of this study was to investigate Cd tolerance of creeping bentgrass (Agrostis stolonifera) and transgenic yeast (Saccharomyces cerevisiae) or Arabidopsis thaliana overexpressing AsGAD1. The Cd-tolerant creeping bentgrass cultivar LOFTSL-93 accumulated more endogenous GABA in relation to a significant upregulation of AsGAD1 in leaf and root than the Cd-sensitive W66569 in response to Cd stress. The overexpression of AsGAD1 significantly enhanced Cd tolerance of yeast or A. thaliana associated with improved endogenous GABA content, low oxidative damage, and high cell membrane stability and photochemical efficiency. Compared with wild type, AsGAD1-overexpressing plants or the atgad1 mutant maintained significantly lower or higher Cd content in leaf and root by down-regulating or up-regulating transcript levels of AtNRAMP1/2/3/4/5 and AtZIP1/2, respectively. Moreover, overexpression of AsGAD1 significantly up-regulated transcript levels of AtHMA1/3, contributing to better Cd compartmentalization from chloroplast into cytoplasm and then into vacuoles. AsGAD1 overexpression also induced expressions of AsMT1A/1B/1C/2/3, AsGSH1/2, and AsPCS1/2, indicating better capacity of Cd chelation in cytosol and vacuoles for Cd detoxification. Hence, AsGAD1-regulated detoxification mechanism of Cd could be related to Cd uptake, transport, and chelation. In addition, lipid contents (PC, PG, and DGDG) and the DGDG/MGDG and PC/PG ratios were improved by the AsGAD1 overexpression, which favors membrane stability and functionality under Cd stress. These findings provide new insight into the regulatory role of GAD1 in Cd tolerance in plants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70063"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0