Physiologia plantarum最新文献

{"title":"Genome‐wide association analysis identifies candidates of three bulb traits in garlic","authors":"Fu Li, Yanzhou Wang, Hassan H. A. Mostafa, Taotao Wang, Siyuan Zhu, Meng Yuan, Song Gao, Touming Liu","doi":"10.1111/ppl.14523","DOIUrl":"https://doi.org/10.1111/ppl.14523","url":null,"abstract":"Garlic bulbs generally possess several swelling cloves, and the swelling degree of the bulbs determines its yield and appearance quality. However, the genetic basis underlying bulb traits remains poorly known. To address this issue, we performed a genome‐wide association analysis for three bulb traits: bulb weight, diameter, and height. It resulted in the identification of 51 significant associated signals from 38 genomic regions. Twelve genes from the associated regions, whose transcript abundances in the developmental bulb showed significant correlations with the investigated traits in 81 garlic accessions, were considered the candidates of the corresponding locus. We focused on five of these candidates and their variations and revealed that the promoter variations of fructose‐bisphosphate aldolase‐encoding <jats:italic>Asa8G05696.1</jats:italic> and beta‐fructofuranosidase‐encoding <jats:italic>Asa6G01167.1</jats:italic> are responsible for the functional diversity of these two genes in garlic population. Interestingly, our results revealed that all candidates we focused on experienced a degree of selection during garlic evolutionary history, and different genotypes of them were retained in two China‐cultivated garlic groups. Taken together, these results suggest a potential involvement of those candidates in the parallel evolution of garlic bulb organs in two China‐cultivated garlic groups. This study provides important insights into the genetic basis of garlic bulb traits and their evolution.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Withania somnifera osmotin (WsOsm) confers stress tolerance in tobacco and establishes novel interactions with the defensin protein (WsDF)","authors":"Varinder Singh, Vipin Hallan, Pratap Kumar Pati","doi":"10.1111/ppl.14513","DOIUrl":"https://doi.org/10.1111/ppl.14513","url":null,"abstract":"Pathogenesis‐related proteins (PR), including osmotins, play a vital role in plant defense, being activated in response to diverse biotic and abiotic stresses. Despite their significance, the mechanistic insights into the role of osmotins in plant defense have not been extensively explored. The present study explores the cloning and characterization of the osmotin gene (<jats:italic>WsOsm</jats:italic>) from <jats:italic>Withania somnifera</jats:italic>, aiming to illuminate its role in plant defense mechanisms. Quantitative real‐time PCR analysis revealed significant induction of <jats:italic>WsOsm</jats:italic> in response to various phytohormones e.g. abscisic acid, salicylic acid, methyl jasmonate, brassinosteroids, and ethrel, as well as biotic and abiotic stresses like heat, cold, salt, and drought. To further elucidate <jats:italic>WsOsm</jats:italic>'s functional role, we overexpressed the gene in <jats:italic>Nicotiana tabacum</jats:italic>, resulting in heightened resistance against the <jats:italic>Alternaria solani</jats:italic> pathogen. Additionally, we observed enhancements in shoot length, root length, and root biomass in the transgenic tobacco plants compared to wild plants. Notably, the <jats:italic>WsOsm‐</jats:italic> overexpressing seedlings demonstrated improved salt and drought stress tolerance, particularly at the seedling stage. Confocal histological analysis of H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> and biochemical studies of antioxidant enzyme activities revealed higher levels in the <jats:italic>WsOsm</jats:italic> overexpressing lines, indicating enhanced antioxidant defense. Furthermore, a pull‐down assay and mass spectrometry analysis revealed a potential interaction between WsOsm and defensin, a known antifungal PR protein (WsDF). This suggests a novel role of WsOsm in mediating plant defense responses by interacting with other PR proteins. Overall, these findings pave the way for potential future applications of <jats:italic>WsOsm</jats:italic> in developing stress‐tolerant crops and improving plant defense strategies against pathogens.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in leaf lifespan, nitrogen resorption, and mean residence time of leaf nitrogen along a soil fertility gradient in an evergreen oak tree","authors":"Shimpei Oikawa","doi":"10.1111/ppl.14519","DOIUrl":"https://doi.org/10.1111/ppl.14519","url":null,"abstract":"The ability of plants to retain nitrogen (N) for a long period of time is critical to their N use efficiency, growth, and fitness, particularly in infertile environments. The mean residence time of leaf N (MRT<jats:sub>L</jats:sub>) and its two determinants, leaf lifespan and N resorption efficiency (<jats:italic>r</jats:italic><jats:sub>N</jats:sub>, the fraction of the total leaf N pool that is resorbed during leaf senescence), have been hypothesized to increase plastically with decreasing soil N fertility but this remains to be fully tested. To avoid confusion by random changes in these characteristics in a relatively narrow N fertility range, MRT<jats:sub>L</jats:sub>, leaf lifespan, and N resorption efficiency were measured in <jats:italic>Quercus glauca</jats:italic> over a broad N fertility range. In the high to moderate N fertility range, leaf lifespan and <jats:italic>r</jats:italic><jats:sub>N</jats:sub> increased with decreasing N addition rate, and thus the MRT<jats:sub>L</jats:sub> increased. However, in the moderate to low N fertility range, leaf lifespan increased but <jats:italic>r</jats:italic><jats:sub>N</jats:sub> decreased significantly, so MRT<jats:sub>L</jats:sub> decreased. The decrease in <jats:italic>r</jats:italic><jats:sub>N</jats:sub> occurred because the senesced leaf N concentration was almost constant at the lower limit while the green leaf N concentration decreased in this range. The hump‐shaped quadratic responses of MRT<jats:sub>L</jats:sub> and <jats:italic>r</jats:italic><jats:sub>N</jats:sub> along the N fertility gradient suggest that incorrect conclusions about the response of these traits to N fertility variation may be drawn from experiments that include only a few fertility levels, and N recycling within leaf canopy alone cannot achieve efficient N use in infertile environments.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi‐omics integration analysis reveals the molecular mechanisms of drought adaptation in homologous tetraploid alfalfa(Medicago sativa ‘Xinjiang‐Daye’)","authors":"Jianwei Qi, Yongzhong Luo, Songsong Lu, Hui Liu, Haixia Huang, Yingde Qiu, Xiaotong Zhou, Chao Ma","doi":"10.1111/ppl.14476","DOIUrl":"https://doi.org/10.1111/ppl.14476","url":null,"abstract":"Drought stress is a predominant abiotic factor leading to decreased alfalfa yield. Genomic ploidy differences contribute to varying adaptation mechanisms of different alfalfa cultivars to drought conditions. This study employed a multi‐omics approach to characterize the molecular basis of drought tolerance in a tetraploid variant of alfalfa (<jats:italic>Medicago sativa</jats:italic>, Xinjiang‐Daye). Under drought treatment, a total of 4446 genes, 859 proteins, and 524 metabolites showed significant differences in abundance. Integrative analysis of the multi‐omics data revealed that regulatory modules involved in flavonoid biosynthesis, plant hormone signalling transduction, linoleic acid metabolism, and amino acid biosynthesis play crucial roles in alfalfa adaptation to drought stress. The severity of drought led to the substantial accumulation of flavonoids, plant hormones, free fatty acids, amino acids, and their derivatives in the leaves. Genes such as <jats:italic>PAL</jats:italic>, <jats:italic>4CL</jats:italic>, <jats:italic>CHI</jats:italic>, <jats:italic>CHS</jats:italic>, <jats:italic>PP2C</jats:italic>, <jats:italic>ARF_3</jats:italic>, and <jats:italic>AHP_4</jats:italic> play pivotal regulatory roles in flavonoid biosynthesis and hormone signalling pathways. Differential expression of the <jats:italic>LOX</jats:italic> gene emerged as a key factor in the elevated levels of free fatty acids. Upregulation of <jats:italic>P5CS_1</jats:italic> and <jats:italic>GOT1/2</jats:italic> contributed significantly to the accumulation of Pro and Phe contents. <jats:italic>ERF19</jats:italic> emerged as a principal positive regulator governing the synthesis of the aforementioned compounds. Furthermore, observations suggest that Xinjiang‐Daye alfalfa may exhibit widespread post‐transcriptional regulatory mechanisms in adapting to drought stress. The study findings unveil the critical mechanisms by which Xinjiang‐Daye alfalfa adapts to drought stress, offering novel insights for the improvement of alfalfa germplasm resources.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription factor DcbZIPs regulate secondary metabolism in Dendrobium catenatum during cold stress","authors":"Xiaohui Zhou, Chenfei Lu, Fenfen Zhou, Yanqin Zhu, Wu Jiang, Aicun Zhou, Yanghui Shen, Lanying Pan, Aimin Lv, Qingsong Shao","doi":"10.1111/ppl.14501","DOIUrl":"https://doi.org/10.1111/ppl.14501","url":null,"abstract":"Cold stress seriously affects plant development and secondary metabolism. The basic region/leucine zipper (bZIP) is one of the largest transcription factor (TFs) family and widely involved in plant cold stress response. However, the function of bZIP in <jats:italic>Dendrobium catenatum</jats:italic> has not been well‐documented. Cold inhibited the growth of <jats:italic>D. catenatum</jats:italic> and increased total polysaccharide and alkaloid contents in stems. Here, 62 DcbZIP genes were identified in <jats:italic>D. catenatum</jats:italic>, which were divided into 13 subfamilies. Among them, 58 DcbZIPs responded to cold stress, which were selected based on the transcriptome database produced from cold‐treated <jats:italic>D. catenatum</jats:italic> seedlings. Specifically, the expression of <jats:italic>DcbZIP3/6/28</jats:italic> was highly induced by cold treatment in leaves or stems. Gene sequence analysis indicated that DcbZIP3/6/28 contains the bZIP conserved domain and is localized to the cell nucleus. Co‐expression networks showed that <jats:italic>DcbZIP6</jats:italic> was significantly negatively correlated with <jats:italic>PAL2</jats:italic> (palmitoyl‐CoA), which is involved in flavonoid metabolism. Moreover, <jats:italic>DcbZIP28</jats:italic> has significant negative correlations with various metabolism‐related genes in the polysaccharide metabolic pathway, including <jats:italic>PFKA1</jats:italic> (6‐phosphofructokinase), <jats:italic>ALDO2</jats:italic> (aldose‐6‐phosphate reductase) and <jats:italic>SCRK5</jats:italic> (fructokinase). These results implied that DcbZIP6 or DcbZIP28 are mainly involved in flavonoid or polysaccharide metabolism. Overall, these findings provide new insights into the roles of the DcbZIP gene family in secondary metabolism in <jats:italic>D. catenatum</jats:italic> under cold stress.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of melatonin and glycine betaine on seed germination, seedling growth, and biochemical attributes of maize under salinity stress","authors":"Wennan Su, Jiaoqi Qiu, Walid Soufan, Ayman El Sabagh","doi":"10.1111/ppl.14514","DOIUrl":"https://doi.org/10.1111/ppl.14514","url":null,"abstract":"Salinity stress represents a major threat to crop production by inhibiting seed germination, growth of seedlings, and final yield and, therefore, to the social and economic prosperity of developing countries. Recently, plant growth‐promoting substances have been widely used as a chemical strategy for improving plant resilience towards abiotic stresses. This study aimed to determine whether melatonin (MT) and glycine betaine (GB) alone or in combination could alleviate the salinity‐induced impacts on seed germination and growth of maize seedlings. Increasing NaCl concentration from 100 to 200 mM declined seed germination rate (4.6–37.7%), germination potential (24.5–46.7%), radical length (7.7–40.0%), plumule length (2.2–35.6%), seedling fresh (1.7–41.3%) and dry weight (23.0–56.1%) compared to control (CN) plants. However, MT and GB treatments lessened the adverse effects of 100 and 150 mM NaCl and enhanced germination comparable to control plants. In addition, results from the pot experiments show that 200 mM NaCl stress disrupted the osmotic balance and persuaded oxidative stress, presented by higher electrolyte leakage, hydrogen peroxide, superoxide radicals, and malondialdehyde compared to control plants. However, compared to the NaCl treatment, NaCl+MT+GB treatment decreased the accumulation of malondialdehyde (24.2–42.1%), hydrogen peroxide (36.2–44.0%), and superoxide radicals (20.1–50.9%) by up‐regulating the activity of superoxide dismutase (28.4–51.2%), catalase (82.2–111.5%), ascorbate peroxidase (40.3–59.2%), and peroxidase (62.2–117.9%), and by enhancing osmolytes accumulation, thereby reducing NaCl‐induced oxidative damages. Based on these findings, the application of MT+GB is an efficient chemical strategy for improving seed germination and growth of seedlings by improving the physiological and biochemical attributes of maize under 200 mM NaCl stress.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catalytic selectivity and evolution of cytochrome P450 enzymes involved in monoterpene indole alkaloids biosynthesis","authors":"Zhan Liu, Jing Pang, Yi Li, Daijing Wei, Jing Yang, Xuefei Wang, Yinggang Luo","doi":"10.1111/ppl.14515","DOIUrl":"https://doi.org/10.1111/ppl.14515","url":null,"abstract":"Cytochrome P450 enzyme (CYP)‐catalyzed functional group transformations are pivotal in the biosynthesis of metabolic intermediates and products, as exemplified by the CYP‐catalyzed C7‐hydroxylation and the subsequent C7‐C8 bond cleavage reaction responsible for the biosynthesis of the well‐known antitumor monoterpene indole alkaloid (MIA) camptothecin. To determine the key amino acid residues responsible for the catalytic selectivity of the CYPs involved in MIA biosynthesis, we characterized the enzymes CYP72A728 and CYP72A729 as stereoselective 7‐deoxyloganic acid 7‐hydroxylases (7DLHs). We then conducted a comparative analysis of the amino acid sequences and the predicted structures of the CYP72A homologs involved in camptothecin biosynthesis, as well as those of the CYP72A homologs implicated in the pharmaceutically significant MIAs biosynthesis in <jats:italic>Catharanthus roseus</jats:italic>. The crucial amino acid residues for the catalytic selectivity of the CYP72A‐catalyzed reactions were identified through fragmental and individual residue replacement, catalytic activity assays, molecular docking, and molecular dynamic simulations analysis. The fragments 1 and 3 of CYP72A565 were crucial for its C7‐hydroxylation and C7‐C8 bond cleavage activities. Mutating fragments 1 and 2 of CYP72A565 transformed the bifunctional CYP72A565 into a monofunctional 7DLH. Evolutionary analysis of the CYP72A homologs suggested that the bifunctional CYP72A in MIA‐producing plants may have evolved into a monofunctional CYP72A. The gene pairs <jats:italic>CYP72A728‐CYP72A610</jats:italic> and <jats:italic>CYP72A729‐CYP72A565</jats:italic> may have originated from a whole genome duplication event. This study provides a molecular basis for the CYP72A‐catalyzed hydroxylation and C‐C bond cleavage activities of CYP72A565, as well as evolutionary insights of CYP72A homologs involved in MIAs biosynthesis.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Native arbuscular mycorrhizal fungi drive ecophysiology through phenotypic integration and functional plasticity under the Sonoran desert conditions","authors":"Alberto Jiménez, Aldo Gutiérrez, Antonio Orozco, Georgina Vargas, Idaly Morales, Esteban Sánchez, Ezequiel Muñoz, Francisco Soto, Miguel Ángel Martínez‐Téllez, Martín Esqueda","doi":"10.1111/ppl.14521","DOIUrl":"https://doi.org/10.1111/ppl.14521","url":null,"abstract":"Knowledge is scarce to what extent environmental drivers and native symbiotic fungi in soil induce abrupt (short‐term), systemic (multiple traits), or specific (a subset of traits) shifts in <jats:italic>C</jats:italic><jats:sub><jats:italic>3</jats:italic></jats:sub> plants' ecophysiological/mycorrhizal responses. We cultivated an emblematic native <jats:italic>C</jats:italic><jats:sub><jats:italic>3</jats:italic></jats:sub> species (<jats:italic>Capsicum annuum</jats:italic> var<jats:italic>. glabriusculum</jats:italic>, “Chiltepín”) to look at how the extreme heat of the Sonoran desert, sunlight regimes (low = 2, intermediate = 15, high = 46 mol m<jats:sup>2</jats:sup> d<jats:sup>−1</jats:sup>) and density of native arbuscular mycorrhizal fungi in soil (low AMF = 1% v/v, high AMF = 100% v/v), drive shifts on mycorrhizal responses through multiple functional traits (106 traits). The warming thresholds were relentlessly harsh even under intensive shade (e.g. superheat maximum thresholds reached ranged between 47–63°C), and several pivotal traits were synergistically driven by AMF (e.g. photosynthetic capacity, biomass gain/allometry, and mycorrhizal colonization traits); whereas concurrently, sunlight regimes promoted most (76%) alterations in functional acclimation traits in the short‐term and opposite directions (e.g. survival, phenology, photosynthetic, carbon/nitrogen economy). Multidimensional reduction analysis suggests that the AMF promotes a synergistic impact on plants' phenotypic integration and functional plasticity in response to sunlight regimes; however, complex relationships among traits suggest that phenotypic variation determines the robustness degree of ecophysiological/mycorrhizal phenotypes between/within environments. Photosynthetic canopy surface expansion, Rubisco activity, photosynthetic nitrogen allocation, carbon gain, and differential colonization traits could be central to plants' overall ecophysiological/mycorrhizal fitness strengthening. In conclusion, we found evidence that a strong combined effect among environmental factors in which AMF are key effectors could drive important trade‐offs on plants' ecophysiological/mycorrhizal fitness in the short term.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of gnotobiotic fermentation on global gene expression of germ-free vegetables.","authors":"Yujin Kim, Hojun Sung, Yeon Bee Kim, Hye Seon Song, Mi-Ja Jung, Jisu Lee, Min Ji Lee, Se Hee Lee, Seong Woon Roh, Jin-Woo Bae, Tae Woong Whon","doi":"10.1111/ppl.14502","DOIUrl":"https://doi.org/10.1111/ppl.14502","url":null,"abstract":"<p><p>Existing research has underscored the vital interplay between host organisms and their associated microbiomes, which affects health and function. In both plants and animals, host factors critically shape microbial communities and influence growth, health, and immunity. Post-harvest plants, such as those used in kimchi, a traditional Korean dish, offer a unique avenue for exploring host-microbe dynamics during fermentation. Despite the emphasis on lactic acid bacteria (LAB) in fermentation studies, the roles of host factors remain unclear. This study aimed to investigate the influence of these factors on plant transcriptomes during kimchi fermentation. We individually inoculated nine LAB strains into germ-free kimchi to generate LAB-mono-associated gnotobiotic kimchi and performed RNA-sequencing analysis for the host vegetables during fermentation. The transcriptomes of post-harvest vegetables in kimchi change over time, and microbes affect the transcriptome profiles of vegetables. Differentially expressed gene analyses revealed that microbes affected the temporal expression profiles of several genes in the plant transcriptomes in unique directions depending on the introduced LAB strains. Cluster analysis with other publicly available transcriptomes of post-harvest vegetables and fruits further revealed that the plant transcriptome is more profoundly influenced by the environment harboring the host than by host phylogeny. Our results bridge the gap in understanding the bidirectional relationship between host vegetables and microbes during food fermentation, illuminating the complex interplay between vegetable transcriptomes, fermentative microbes, and the fermentation process in food production. The different transcriptomic responses elicited by specific LAB strains suggest the possibility of microbial manipulation to achieve the desired fermentation outcomes.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intricate microbe-plant-metabolic remodeling mediated by intercropping enhances the quality of Panax quinquefolius L.","authors":"Wanying Duan, Xiaoli Chen, Yu Ding, Xinying Mao, Zhengjian Song, Jie Bao, Lei Fang, Lanping Guo, Jie Zhou","doi":"10.1111/ppl.14499","DOIUrl":"https://doi.org/10.1111/ppl.14499","url":null,"abstract":"<p><p>Improving the cultivation mode and technology for traditional Chinese medicine has become important for its sustainable development. Monoculture enhances plant diseases, which decreases yield and quality. Intercropping is an effective measure to counterbalance that negative effect. In this study, we focused on Panax quinquefolium L. (ginseng) and four treatments were set up: the control without intercropping, P. quinquefolius + ryegrass (Lolium perenne L.), P. quinquefolius + red clover (Trifolium pratense L.), and P. quinquefolius + ryegrass + red clover. An LC-MS/MS system was used to detect the changes in the P. quinquefolius secondary metabolites, and high-throughput sequencing technology was used to determine the changes in the P. quinquefolius' rhizosphere soil microorganisms. Ginsenoside content, soil enzyme activities, and arbuscular mycorrhizal infection rate of P. quinquefolius were also measured using HPLC, ELISA kits, and microscopy, respectively. Co-intertia and Pearson's analysis were performed to explore the relationship between the metabolites and the P. quinquefolius microorganisms. Intercropping significantly increased the content of ginsenoside metabolites and recruited a large number of beneficial bacteria to the P. quinquefolius rhizosphere. The P. quinquefolius secondary metabolites were associated with the rhizosphere microbial community. For example, the dominant microorganisms, such as Acidobacteriota and Chloroflexi, played a key role in promoting the synthesis of ginsenoside Rd and (20R) ginsenoside Rg3 by P. quinquefolius. Intercropping led to changes in the P. quinquefolius secondary metabolites by driving and reshaping the rhizosphere microorganisms. These findings revealed the potential application of intercropping for improving the quality of P. quinquefolius.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142110905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}