Physiologia plantarum最新文献_第2页

Transcriptome Analysis Reveals Far-Red Light's Effect on the Seedling Leaf Angle of Rice (Oryza sativa L.). 远红光对水稻幼苗叶片角度的影响。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2026-05-01 DOI: 10.1111/ppl.70906

Xiaojuan Zhang, Chengzhu Tang, Sha Ouyang, Yanan Li, Zhe Yang, Xiyu Zhou, Arcel Mulowayi Mutombo, Xionglun Liu, Zhi Zhou, Xiangyang Lu, Mao Xia

{"title":"Transcriptome Analysis Reveals Far-Red Light's Effect on the Seedling Leaf Angle of Rice (Oryza sativa L.).","authors":"Xiaojuan Zhang, Chengzhu Tang, Sha Ouyang, Yanan Li, Zhe Yang, Xiyu Zhou, Arcel Mulowayi Mutombo, Xionglun Liu, Zhi Zhou, Xiangyang Lu, Mao Xia","doi":"10.1111/ppl.70906","DOIUrl":"https://doi.org/10.1111/ppl.70906","url":null,"abstract":"Far-red light significantly affects plant growth, including seedling development, root growth, and leaf angle, but the regulatory mechanisms underlying the positioning of the rice leaf angle remain unclear. In this study, eight light quality treatments were applied: white light (W), white light with far-red (W + Fr), red light (R), red light with far-red (R + Fr), blue light (B), blue light with far-red (B + Fr), red-blue light (RB), and red-blue light with far-red (RB + Fr). Additionally, six far-red light ratios (0%, 7.5%, 15%, 30%, 60%, 100%) were used to investigate the molecular mechanisms by which far-red light modulates leaf angles in rice seedlings. Compared to treatments without far-red light, supplemental far-red light significantly increased leaf angle and markedly decreased plant height, enhanced seedling quality, increased specific leaf weight, promoted root growth and photosynthetic capacity, and overall improved seedling performance. Moreover, far-red light markedly increased the leaf angle of rice seedlings. As the far-red proportion increased, leaf angle first increased and then decreased, a pattern consistent with the anatomical observations. Transcriptome analysis and WGCNA identified RPL5 as a key candidate gene involved in leaf angle regulation. However, the functional role of RPL5 and its regulatory mechanisms on leaf angles require further experimental validation. This study provides a theoretical basis for understanding how light and auxin signals coordinate to regulate rice leaf angles in response to far-red light.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 3","pages":"e70906"},"PeriodicalIF":3.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147778571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Characterization of MeJA-Induced OjTIFY2 in Mediating Salt Stress Tolerance in Oenanthe javanica. meja诱导的OjTIFY2介导水芹耐盐胁迫的功能研究

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2026-05-01 DOI: 10.1111/ppl.70926

Shuping Zhao, Xiaojing Fan, Fenghua Li, Shangjie Chen, Qianru Niu, Peng Wu, Kai Feng, Liangjun Li

{"title":"Functional Characterization of MeJA-Induced OjTIFY2 in Mediating Salt Stress Tolerance in Oenanthe javanica.","authors":"Shuping Zhao, Xiaojing Fan, Fenghua Li, Shangjie Chen, Qianru Niu, Peng Wu, Kai Feng, Liangjun Li","doi":"10.1111/ppl.70926","DOIUrl":"https://doi.org/10.1111/ppl.70926","url":null,"abstract":"The plant-specific transcription factor TIFY plays key roles in plant growth and development, including seed germination, signal transduction, and responses to environmental stimuli and plant hormones. However, few studies have investigated the function of the TIFY family in responding to salinity stress in water dropwort (Oenanthe javanica (Blume) D.C). Here, a total of 24 OjTIFY genes, named OjTIFY1-OjTIFY24, were identified in water dropwort. And their phylogenetic relationships, gene structure, chromosomal distribution, promoter regions, and collinearity patterns were comprehensively analyzed. We further identified a member, OjTIFY2, located in the nucleus and positively upregulated by exogenous MeJA. Meanwhile, we found that exogenous MeJA could alleviate the sensitivity of water dropwort to salt stress by regulating the activity of antioxidant enzymes and the content of osmotic regulatory substances. A transient overexpression experiment in water dropwort showed that OjTIFY2 enhanced its tolerance to salt stress. The qRT-PCR analysis showed that OjSOS1, OjSOS3, OjKAT1, OjP5CS, and OjHKT1 were significantly upregulated when OjTIFY2-overexpressing lines were subjected to salt stress. Then, the transgenic Arabidopsis overexpressing OjTIFY2 also exhibited tolerance to salt stress by increasing the activities of SOD, POD, and CAT, and the contents of proline and chlorophyll, and by reducing the contents of MDA and H2O2. We also found that OjTIFY2 positively regulates the expression of salt-stress-related genes AtSOS1, AtSOS3, AtKAT1, AtP5CS, and AtHKT1. These results suggest that OjTIFY2 acts as a positive regulator of salt tolerance in water dropwort by modulating ROS scavenging and the expression of salt-related genes.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 3","pages":"e70926"},"PeriodicalIF":3.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clearing the Noise: Seasonal Dynamics of Endophytic Bacteria in Fagus sylvatica Leaves Revealed by Application of PNA Clamps. 清除噪音：应用PNA夹揭示的山毛榉叶片内生细菌的季节动态。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2026-05-01 DOI: 10.1111/ppl.70897

Irene Giubilei, Silvia Turco, Antonella Cardacino, Lovely Mahawar, Benedicte Riber Albrectsen, Angelo Mazzaglia

{"title":"Clearing the Noise: Seasonal Dynamics of Endophytic Bacteria in Fagus sylvatica Leaves Revealed by Application of PNA Clamps.","authors":"Irene Giubilei, Silvia Turco, Antonella Cardacino, Lovely Mahawar, Benedicte Riber Albrectsen, Angelo Mazzaglia","doi":"10.1111/ppl.70897","DOIUrl":"https://doi.org/10.1111/ppl.70897","url":null,"abstract":"The characterization of the seasonal dynamics of endophytic bacteria in beech leaves can be hindered by co-amplification of chloroplast and mitochondrial plant DNA. This study applies established peptide nucleic acid (PNA) clamps to suppress host-derived amplification while resolving bacterial succession across the vegetative season. Chloroplast- and mitochondrion-specific PNAs inverted the proportion of host to bacterial reads, enabled the recovery of bacterial sequence variants, and increased alpha diversity accordingly. Beta-diversity analyses showed that, once host contamination was removed, samples displayed a clear seasonal trajectory. Early-season leaves contained high abundances of Pseudomonas together with taxa likely introduced through plant-insect-microbe interactions. As leaves matured, the microbiome shifted toward a more stable composition dominated by well-established genera. The transition from early transient taxa to the later enrichment of phyllosphere-adapted and nutrient-cycling genera demonstrates that beech leaves host a temporally structured microbiome shaped by leaf development and seasonal environmental stress.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 3","pages":"e70897"},"PeriodicalIF":3.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147778516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Weighted Gene Co-Expression Network Analysis Reveals Hub Genes Associated With Response to Low-Phosphorus Stress in Pueraria lobata. 加权基因共表达网络分析揭示葛根对低磷胁迫的响应相关枢纽基因。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2026-05-01 DOI: 10.1111/ppl.70912

Yanhua Tang, Shuwei Zhang, Yi Xi, Lili Zhao, Chao Chen

{"title":"Weighted Gene Co-Expression Network Analysis Reveals Hub Genes Associated With Response to Low-Phosphorus Stress in Pueraria lobata.","authors":"Yanhua Tang, Shuwei Zhang, Yi Xi, Lili Zhao, Chao Chen","doi":"10.1111/ppl.70912","DOIUrl":"https://doi.org/10.1111/ppl.70912","url":null,"abstract":"Phosphorus is an essential element for plant growth, and its deficiency severely limits crop productivity. To explore genetic resources for improving phosphorus use efficiency, this study investigated the differential low-phosphorus tolerance mechanisms of two kudzu (Pueraria lobata) germplasms from Australia (tolerant) and Jiangsu, China (sensitive) using hydroponics, RNA-seq, and WGCNA. The results showed that the Australian germplasm exhibited superior low-phosphorus tolerance through root morphological plasticity, which was characterized by increased root length and tip number under low phosphorus (0.05 mmol L-1 KH2PO4); enhanced reactive oxygen species scavenging, with higher peroxidase and catalase activities under extremely low phosphorus (0.005 mmol L-1 KH2PO4), and extensive transcriptome reprogramming, including 8896 upregulated genes in response to phosphorus deficiency. In contrast, the Jiangsu germplasm showed limited adaptive responses, with reduced root hairs and biomass under stress. WGCNA partitioned 21,734 expressed genes into 20 co-expression modules, among which the turquoise and light green modules showed significant correlations with phosphorus treatments and phenotypic traits. Genes in the turquoise module were primarily enriched in oxidative phosphorylation and phenylpropanoid biosynthesis pathways, whereas the light green module was significantly enriched in ribosome-related pathways. Five hub genes, ABCG5, TALDO, VAMP7B, EEF1AS, and RPLP0, were identified as core components of these modules. Collectively, these findings establish the Australian kudzu as a valuable germplasm resource for improving phosphorus use efficiency in crops and provide key molecular targets for precision breeding.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 3","pages":"e70912"},"PeriodicalIF":3.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Multivariate and Univariate Analysis of Circadian Metabolomic Signatures in Yerba Mate Clones in a Semi-Hydroponic System. 半水培系统中马黛茶无性系昼夜代谢组学特征的多因素和单因素综合分析。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2026-05-01 DOI: 10.1111/ppl.70917

Tamires O Melo, Joachim Kopka, Ivar Wendling, Alexander Erban, Francisco A Marques, Fabricio A Hansel

{"title":"Integrated Multivariate and Univariate Analysis of Circadian Metabolomic Signatures in Yerba Mate Clones in a Semi-Hydroponic System.","authors":"Tamires O Melo, Joachim Kopka, Ivar Wendling, Alexander Erban, Francisco A Marques, Fabricio A Hansel","doi":"10.1111/ppl.70917","DOIUrl":"https://doi.org/10.1111/ppl.70917","url":null,"abstract":"This study presents an integrated multivariate and univariate analysis of circadian metabolomic signatures in Ilex paraguariensis (yerba mate) clones cultivated under semi-hydroponic conditions. Using repeated measures ANOVA-Simultaneous Component Analysis (RM-ASCA+) and hierarchical clustering on principal components (HCPC), we explored clone-specific and photoperiod-dependent metabolic responses across light and dark phases, complemented by high-resolution timepoint sampling (HRS) and targeted screening. Clone EC21 exhibited elevated levels of sugars, amino acids, and organic acids, suggesting a metabolic strategy adapted to saline stress and nocturnal energy demands. Photoperiod effects revealed circadian regulation of central carbon metabolites (e.g., glucose, fructose, maltose) and phenylpropanoid intermediates linked to bioactive compounds such as caffeoyl-quinic acids. Interaction effects highlighted metabolic plasticity, particularly in nitrogen assimilation, with compounds like 2-oxo-glutaric acid, glutamine, and ornithine showing clone-specific temporal patterns. Caffeine, a heritable and physiologically relevant metabolite, displayed distinct circadian profiles. EC24 accumulated caffeine during the day, while EC21 peaked at night. This dynamic distribution, supported by allantoin patterns in caffeine catabolism, suggests divergent nitrogen turnover strategies between clones. These findings underscore the importance of genotype selection and temporal regulation in optimizing yerba mate performance under semi-hydroponic systems. The combined use of RM-ASCA+ and univariate analysis proved to be a powerful approach for profiling metabolomic rhythms, offering valuable insights for breeding programs targeting bioactive compound enhancement, stress resilience, and metabolic efficiency.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 3","pages":"e70917"},"PeriodicalIF":3.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Genome Identification Analysis of WOX Gene Family and Its Expression in Protopanaxatriol Ginsenosides Under MeJA Treatment in Ginseng. MeJA处理下人参原人参皂苷WOX基因家族的基因组鉴定及表达分析

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2026-05-01 DOI: 10.1111/ppl.70920

Yu Zhang, Aimin Wang, Jun Wu, Yuci Gui, Jiaqing Liu, Kexin Zhang, Mingzhu Zhao, Meiping Zhang, Yi Wang, Zhuo Tian, Kangyu Wang

{"title":"Comprehensive Genome Identification Analysis of WOX Gene Family and Its Expression in Protopanaxatriol Ginsenosides Under MeJA Treatment in Ginseng.","authors":"Yu Zhang, Aimin Wang, Jun Wu, Yuci Gui, Jiaqing Liu, Kexin Zhang, Mingzhu Zhao, Meiping Zhang, Yi Wang, Zhuo Tian, Kangyu Wang","doi":"10.1111/ppl.70920","DOIUrl":"https://doi.org/10.1111/ppl.70920","url":null,"abstract":"Panax ginseng is an important medicinal plant, and its major bioactive components are ginsenosides. The WOX gene family is a plant-specific transcription factor family involved in the regulation of multiple biological processes, including stem cell maintenance, embryonic development, lateral organ development, and organogenesis. In this study, we screened and identified members of the PgWOX gene family using an established ginseng transcriptome and genome database. Subsequently, a comprehensive analysis was performed, including phylogenetic analysis, gene structure characterization, conserved motif identification, cis-acting element prediction, expression pattern analysis, and chromosomal localization analysis. In addition, gene ontology (GO) functional annotation and co-expression network analysis were conducted for PgWOX genes, in which the co-expression correlations between PgWOX genes and the previously published key enzyme genes involved in ginsenoside biosynthesis were investigated. Furthermore, ginseng adventitious roots were treated with methyl jasmonate (MeJA) to study the expression responses of three PgWOX genes that clustered with the known key ginsenoside biosynthesis-related enzyme genes in the co-expression network. The results showed that all three PgWOX genes exhibited distinct expression responses to MeJA. Finally, the contents of five protopanaxatriol-type ginsenosides (Re, Rf, Rg1, Rg2, and Rh1) were determined in ginseng adventitious roots treated with MeJA at various time points, focusing on three PgWOX genes (PgWOX21, PgWOX35, and PgWOX36). The results demonstrated that Re content showed the most significant variation among all detected ginsenosides. This study provides valuable data for subsequent research on members of the WOX gene family in P. ginseng and offers an experimental reference for verifying the functions of these family members in the MeJA signaling pathway.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 3","pages":"e70920"},"PeriodicalIF":3.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coordinated Regulation of Iron Homeostasis, Antioxidant Defenses and Hormone Signaling Underlies Pepper Responses to Iron Deficiency and Excess. 铁稳态、抗氧化防御和激素信号的协调调节是辣椒对铁缺乏和过量反应的基础。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2026-05-01 DOI: 10.1111/ppl.70890

Mingrui Xu, Yuefei Ma, Xiaoxia Zhou, Yali Yang, Fengfeng Dang, Ying Sun

{"title":"Coordinated Regulation of Iron Homeostasis, Antioxidant Defenses and Hormone Signaling Underlies Pepper Responses to Iron Deficiency and Excess.","authors":"Mingrui Xu, Yuefei Ma, Xiaoxia Zhou, Yali Yang, Fengfeng Dang, Ying Sun","doi":"10.1111/ppl.70890","DOIUrl":"https://doi.org/10.1111/ppl.70890","url":null,"abstract":"Iron availability strongly influences crop performance, yet the mechanisms in non-model horticultural species remain unclear. We combined morphological, physiological, and root transcriptome analyses to dissect the response of Capsicum annuum L. cultivar CA#8 to iron deficiency, low iron, and excess iron. Seedlings grown hydroponically under four iron supplies (0, 5, 25, and 150 μM EDTA-Fe) showed distinct shoot and root phenotypes: iron deficiency caused interveinal chlorosis and strong inhibition of root growth, excess iron induced leaf wrinkling with white spots and reduced root development, whereas low iron had milder effects. Under deficiency, rhizosphere pH decreased and ferric chelate reductase activity increased sharply, supporting activation of a Strategy I-type acquisition system. Shoot and root iron contents declined in deficient and low-iron plants but increased under excess iron, while manganese accumulated mainly in deficient shoots and zinc remained largely unchanged. Iron stress altered multiple hormones and triggered transient increases in superoxide dismutase, peroxidase, and catalase activities, consistent with regulated adjustments of the reactive oxygen species homeostasis. Root RNA-seq at two days identified 1822, 436, and 1211 differentially expressed genes under deficiency, low iron, and excess iron, respectively. Enrichment and co-expression analyses highlighted a coordinated induction or repression of iron transporters and regulators, including IRT- and NRAMP-like uptake components, YSL- and VTL-like transporters, and transcription factors and hormone-related genes linked to iron and oxidative signaling. Research indicates that pepper plants maintain iron homeostasis by integrating proton extrusion, reduction capacity, metal transport, vacuolar sequestration, and antioxidant defense mechanisms to limit damage under both iron deficiency and excess.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 3","pages":"e70890"},"PeriodicalIF":3.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147778464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Self-Cleavage-Dependent Activation Mechanism of the Effector RipBH Associated With Bacterial Wilt and Tuber Rot in Potato. 马铃薯枯萎病和块茎腐病相关效应物RipBH的自裂解激活机制。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2026-05-01 DOI: 10.1111/ppl.70887

Xueao Zheng, Huishan Qiu, Mengshu Huang, Xiaodan Tan, Yanping Li, Hao Xue, Dong Cheng, Wenhao Li, Qian Li, Botao Song, Huilan Chen, Juan Du

{"title":"A Self-Cleavage-Dependent Activation Mechanism of the Effector RipBH Associated With Bacterial Wilt and Tuber Rot in Potato.","authors":"Xueao Zheng, Huishan Qiu, Mengshu Huang, Xiaodan Tan, Yanping Li, Hao Xue, Dong Cheng, Wenhao Li, Qian Li, Botao Song, Huilan Chen, Juan Du","doi":"10.1111/ppl.70887","DOIUrl":"https://doi.org/10.1111/ppl.70887","url":null,"abstract":"Homeostatic regulation of proteolytic activity is fundamental to plant cellular physiology, and dysregulated protease-like activities are frequently associated with cytotoxic or stress-induced cell death. Here, we identify RipBH, a previously uncharacterized type III-secreted protein from Ralstonia solanacearum, as an intracellular self-cleaving protease-like effector with the capacity to perturb host physiological balance. RipBH harbors a papain-like catalytic core and multiple ankyrin repeats; structural mutagenesis showed that conserved catalytic residues (C135, H244, D268, and N117) are indispensable for self-processing and cell death-inducing activity. RipBH undergoes auto-cleavage inside plant cells, producing smaller fragments that are detectable in both the cytoplasm and nucleus. Truncation of ankyrin repeats altered cleavage behavior and abolished cell-death induction, supporting the idea that ankyrin-mediated structural constraints function as a regulatory module required for activation. Importantly, RipBH-induced necrosis occurred largely independently of the tested canonical ETI-related signaling components, suggesting a physiology-centered disruption pathway rather than immune receptor-mediated recognition. We propose that RipBH operates as a pathogen-encoded proteolytic switch that destabilizes intracellular homeostasis, providing a potential mechanistic link between effector auto-processing and necrosis-like physiological collapse under biotic stress. Our findings contribute to the conceptual framework of proteolysis-associated plant cell dysfunction and highlight pathogen-driven interference with core physiological processes.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 3","pages":"e70887"},"PeriodicalIF":3.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147778514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The bZIP Transcription Factor PjbZIP1 Enhances Resistance to Powdery Mildew in Paeonia jishanensis by Directly Activating PjICS Involved in the SA Pathway. bZIP转录因子PjbZIP1通过直接激活参与SA通路的PjICS增强白粉病抗性

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2026-05-01 DOI: 10.1111/ppl.70893

Xiao-Chun Zhao, Min Li, Jia-Yin Niu, Yi-Han Zhang, Yan Ren, Qian Yu, Yi-He Yu

{"title":"The bZIP Transcription Factor PjbZIP1 Enhances Resistance to Powdery Mildew in Paeonia jishanensis by Directly Activating PjICS Involved in the SA Pathway.","authors":"Xiao-Chun Zhao, Min Li, Jia-Yin Niu, Yi-Han Zhang, Yan Ren, Qian Yu, Yi-He Yu","doi":"10.1111/ppl.70893","DOIUrl":"https://doi.org/10.1111/ppl.70893","url":null,"abstract":"Paeonia jishanensis is a wild tree peony germplasm resource in China that has strong resistance to powdery mildew. Domesticated peony varieties exhibit compromised immunity to powdery mildew pathogens. The present study investigated the function of PjbZIP1 through expression analysis, virus-induced gene silencing (VIGS), and overexpression in Arabidopsis. Structural analysis identified a canonical basic region/leucine zipper (bZIP) motif at the C-terminal region of PjbZIP1. In P. jishanensis plants, PjbZIP1 expression was upregulated under powdery mildew infection, exogenous salicylic acid (SA) treatment, and methyl jasmonate exposure. Gene-silenced PjbZIP1 plants exhibited increased susceptibility to powdery mildew, whereas transgenic PjbZIP1 overexpression enhanced resistance. In addition, VIGS and overexpression of PjbZIP1 affected SA synthesis and the induction of genes associated with the plant defense signaling pathway. Among the candidate genes involved in SA-regulated defense responses, PjbZIP1 significantly upregulated the expression of PjICS, which encodes a key enzyme for SA biosynthesis. Yeast one-hybrid, firefly luciferase complementation imaging, and electrophoretic mobility shift assays demonstrated in vitro and in vivo that PjbZIP1 interacts with PjICS. Transcription of PjICS was induced in P. jishanensis infected with powdery mildew and was significantly enhanced in overexpressed PjbZIP1 plants. These findings establish that PjbZIP1 enhances powdery mildew resistance in tree peony by regulating the PjICS-mediated SA signaling defense pathway. These findings establish PjbZIP1 as a critical regulator of SA-mediated resistance to powdery mildew in P. jishanensis, providing genetic tools for breeding disease-resistant cultivars.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 3","pages":"e70893"},"PeriodicalIF":3.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147778547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal Pathogen Activity and Stress-Dependent Responses of Grapevine Wood to Esca and Drought. 葡萄木真菌病原菌活性及对Esca和干旱的胁迫响应。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2026-05-01 DOI: 10.1111/ppl.70914

Marie Chambard, Dario Cantù, Giovanni Bortolami, Ninon Dell'Acqua, Nathalie Ferrer, Gregory A Gambetta, Jadran F Garcia, Pierre Gastou, Mélanie Massonnet, Samuele Moretti, Adam Rochepeau, Pierre Pétriacq, Marie Foulongne-Oriol, Chloé E L Delmas

{"title":"Fungal Pathogen Activity and Stress-Dependent Responses of Grapevine Wood to Esca and Drought.","authors":"Marie Chambard, Dario Cantù, Giovanni Bortolami, Ninon Dell'Acqua, Nathalie Ferrer, Gregory A Gambetta, Jadran F Garcia, Pierre Gastou, Mélanie Massonnet, Samuele Moretti, Adam Rochepeau, Pierre Pétriacq, Marie Foulongne-Oriol, Chloé E L Delmas","doi":"10.1111/ppl.70914","DOIUrl":"https://doi.org/10.1111/ppl.70914","url":null,"abstract":"Biotic and abiotic stresses alter the physiology of perennial plants, with consequences for fungal endophytes and disease expression. In grapevine, drought inhibits esca disease expression, but the underlying molecular interactions between the plant and fungi are unknown. We combined wood metatranscriptomics, metabolomics, and metabarcoding to investigate these interactions in 30-year-old grapevines and eight wood-pathogenic fungi under conditions of drought or esca leaf symptom expression. Both esca and drought decreased grapevine transpiration, but with different underlying mechanisms that induced specific transcriptomic and metabolic signatures. Similar pathways were also activated, including the phenylpropanoid and stilbenoid synthesis pathways. These stress responses could potentially confer cross-tolerance and elicit different fungal molecular responses. Across all fungi, the total level of putative virulence factors increased significantly under both stresses. Under drought, only the relative abundance of Phaeomoniella chlamydospora and gene expression involved in anti-oxidative mechanisms, growth, and reproduction increased. Under esca expression conditions, only the relative abundance of Fomitiporia mediterranea and gene expression involved in wood degradation, competition, detoxification, and growth increased. Under drought, induced grapevine defenses and reduced transpiration, together with the low abundance and putatively weak virulence of F. mediterranea may account for the inhibition of esca leaf symptom.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 3","pages":"e70914"},"PeriodicalIF":3.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13133591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0