Physiologia plantarum最新文献_第2页

The Rootstock's Cotyledon-Regulated Fructokinase ClFRK1 Contributes to Graft Union Formation in Watermelon. 砧木子叶调节果糖激酶ClFRK1对西瓜嫁接结合的影响

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70144

Akebaierjiang Kadeer, Mu Xiong, Chen-le Hou, Bei-Ping Zheng, Hong-Liang Zhang, Xiang-Shuai Wu, Yehia Abouseif, Zu-Hua Zheng, Zhi-Long Bie, Michitaka Notaguchi, Yuan Huang

{"title":"The Rootstock's Cotyledon-Regulated Fructokinase ClFRK1 Contributes to Graft Union Formation in Watermelon.","authors":"Akebaierjiang Kadeer, Mu Xiong, Chen-le Hou, Bei-Ping Zheng, Hong-Liang Zhang, Xiang-Shuai Wu, Yehia Abouseif, Zu-Hua Zheng, Zhi-Long Bie, Michitaka Notaguchi, Yuan Huang","doi":"10.1111/ppl.70144","DOIUrl":"https://doi.org/10.1111/ppl.70144","url":null,"abstract":"Grafting is a traditional horticultural practice that enhances plant resilience against biotic and abiotic stresses. However, the influence of specific tissues, such as rootstock cotyledons, on graft union formation is not well understood. This study investigates the impact of rootstock cotyledon removal on graft healing in watermelon and its underlying mechanisms. Our results indicate that grafting with rootstock cotyledons (+C) consistently resulted in higher survival rates and better growth outcomes compared to grafting without rootstock cotyledons (-C). This effect was more pronounced in cultivated watermelon rootstocks, which have lower hypocotyl sugar content than wild watermelon rootstocks. Transcriptomic analysis revealed that cotyledon removal disrupted sugar metabolism and affected gene expression related to cell division and tissue development. A fructokinase, ClFRK1, was identified among the candidate genes positively correlated with graft survival rate and healing degree. Silencing ClFRK1 reduced callus proliferation, delayed graft healing and reduced survival rate. Conversely, fructose treatment increased ClFRK1 expression levels at the graft junction, which promoted callus proliferation and vascular reconnection. We propose a novel regulatory model for how ClFRK1 regulates graft union formation. These findings underscore new insights into the interactions and synergistic processes between the graft interface and non-grafted organs during graft union formation and also enrich our understanding of fructokinase.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70144"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silicon modulates nitrogen and secondary metabolism in Glycyrrhiza uralensis under drought and salt stress conditions. 干旱和盐胁迫下硅对甘草氮和次生代谢的调节作用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70138

Guohui Chen, Xiaojia Zhang, Gaochang Cui, Wenjin Zhang, Qiuxian Bai, Xinhui Zhang

{"title":"Silicon modulates nitrogen and secondary metabolism in Glycyrrhiza uralensis under drought and salt stress conditions.","authors":"Guohui Chen, Xiaojia Zhang, Gaochang Cui, Wenjin Zhang, Qiuxian Bai, Xinhui Zhang","doi":"10.1111/ppl.70138","DOIUrl":"https://doi.org/10.1111/ppl.70138","url":null,"abstract":"Glycyrrhiza uralensis Fisch (G. uralensis) is a key species for windbreak and sand fixation, possessing notable pharmacological and economic value. However, the yield of G. uralensis is considerably impacted due to its cultivation in arid, semi-arid, and salt-affected regions. Silicon (Si) has been reported to improve plant tolerance to drought and salt stress by regulating nitrogen and secondary metabolism. Herein, the effects of Si treatment on nitrogen and secondary metabolism of G. uralensis seedlings under drought (D), salt (S), and drought-salt (SD) stresses were investigated in combination with physiological and transcriptomic analyses. The results indicated that stress conditions significantly inhibited the growth of G. uralensis seedlings by suppressing nitrogen and secondary metabolism. Si treatment counteracted these inhibitions to some extent. Specifically, Si treatment increased soluble protein content by approximately 15% by regulating the nitrogen metabolism of G. uralensis under D stress. Furthermore, Si treatment elevated the content of glycyrrhetinic acid by about 89% under SD stress by increasing the content of primary metabolites and regulating the expression of enzymes involved in the biosynthesis of glycyrrhizic acid and liquiritin, including 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), squalene synthase (SQS), and β-amyrin synthase (β-AS). In summary, our findings suggest that Si could alleviate the adverse effects induced by drought and/or salt stresses on the growth of G. uralensis seedlings by regulating nitrogen metabolisms, which further triggered the accumulation of secondary metabolites, ultimately improving the stress resistance of cultivated G. uralensis seedlings. This work provides direction for Si to improve stress resistance.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70138"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive analytical method 'Regulatome' revealed a novel pathway for aerenchyma formation under waterlogging in wheat. 一种综合分析方法“调节组”揭示了小麦涝渍条件下通气组织形成的新途径。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70157

Hao Gao, Mingjiong Chen, Nanfei Jin, Lingzhen Ye, Guoping Zhang, Qiufang Shen, Zhengyuan Xu

{"title":"A comprehensive analytical method 'Regulatome' revealed a novel pathway for aerenchyma formation under waterlogging in wheat.","authors":"Hao Gao, Mingjiong Chen, Nanfei Jin, Lingzhen Ye, Guoping Zhang, Qiufang Shen, Zhengyuan Xu","doi":"10.1111/ppl.70157","DOIUrl":"https://doi.org/10.1111/ppl.70157","url":null,"abstract":"Waterlogging is a major abiotic stress restricting crop yield globally, and aerenchyma formation is one of the most important adaptive strategies in waterlogging-tolerant plants. However, the conservation of this process remains poorly understood, and additional pathways are yet to be identified. Here, physiological, anatomical, transcriptomic, and metabolomic analyses were conducted on wheat seedlings under normal and waterlogging conditions. Waterlogging caused growth inhibition and physiological damage, as well as induced aerenchyma formation in roots. A total of 10,346 differentially expressed genes and 3,419 differential metabolites were identified in roots. In addition to the AP2/ERF (APETALA2/ETHYLENE RESPONSIVE FACTOR) gene family, integrating analyses also revealed the role of LOB/AS2 (LATERAL ORGAN BOUNDARIES/ASYMMETRIC LEAVES2) in aerenchyma formation under waterlogging. It was revealed that the classical pathway of aerenchyma formation mediated by ethylene response, as well as synergy of calcium ion and reactive oxygen species, was deeply conserved in both monocots and eudicots during 160 million years of evolution through gene co-expression networks of cross-species. The newly introduced concept 'Regulatome' supported the classical pathway of aerenchyma formation, with a proposed model of the jasmonic acid signalling pathway involved in waterlogging, suggesting its usefulness in gene identification and function exploration. These findings provide a novel insight into the regulatory mechanisms of aerenchyma formation and breeding approaches for developing wheat cultivars with high waterlogging tolerance.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70157"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UV-B orchestration of growth, yield and grain quality traits highlights modifications of source-to-sink relationship in pearl millet cultivars. UV-B对珍珠粟品种生长、产量和品质性状的调控，凸显了源库关系的改变。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70141

Pooja Singh, Krishna Kumar Choudhary

{"title":"UV-B orchestration of growth, yield and grain quality traits highlights modifications of source-to-sink relationship in pearl millet cultivars.","authors":"Pooja Singh, Krishna Kumar Choudhary","doi":"10.1111/ppl.70141","DOIUrl":"https://doi.org/10.1111/ppl.70141","url":null,"abstract":"Climate change and stratospheric ozone layer dynamics have altered the intensity of ultraviolet B (UV-B) radiation, affecting the growth, yield, and metabolic responses of major cereal crops. As a result, to meet the future demand scenario for growing population and health concerns, millets have been recognized as important substitutes. Among them, pearl millet has shown resilience against various abiotic stresses, but its response to UV-B radiation has not yet been explored. Recognizing its importance in present global food systems, the present investigation aimed to analyse the effect of elevated UV-B (eUV-B; ambient+7.2 kJm-2d-1) on four cultivars (HHB-272, HHB-67, MPMH-21, and MPMH-17) of pearl millet during panicle development stage and grain filling stage under natural field conditions. The results indicated that UV-B stress altered growth morphology (plant height, number of leaves, leaf area, and panicle length) at both stages, with less pronounced effects on cultivars HHB-272 and HHB-67. Declined growth indices [relative growth rate (RGR), absolute growth rate (AGR), net assimilation rate (NAR), and leaf area ratio (LAR)] at panicle stage revealed predominance of UV-B stress. Grain yield was positively affected in all the cultivars, indicating better resource allocation to different important needs, thereby altering the trade-offs between growth and development as reported in our present study. The grain quality (total soluble sugars, reducing sugars, total free amino acids, starch content, soluble protein) of harvested seeds exhibited a decreased quality response index, and metabolic pathway analysis of the metabolites identified through UHPLC-HRMS indicated a shift in photoassimilates towards fatty acid biosynthesis. These findings help to understand various plant metabolic pathways, potentially revealing the resilience mechanism involved in pearl millet under eUV-B exposure.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70141"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of nanobodies in Arabidopsis thaliana strengthens the absorption capacity of triclosan from growth media. 纳米体在拟南芥中的表达增强了生长介质对三氯生的吸收能力。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70163

Weixia Liu, Mengya Li, Xiangyu Zhang, Yongqiang Ma, Qing X Li, Christophe Morisseau, Bruce D Hammock, Ting Xu

{"title":"Expression of nanobodies in Arabidopsis thaliana strengthens the absorption capacity of triclosan from growth media.","authors":"Weixia Liu, Mengya Li, Xiangyu Zhang, Yongqiang Ma, Qing X Li, Christophe Morisseau, Bruce D Hammock, Ting Xu","doi":"10.1111/ppl.70163","DOIUrl":"https://doi.org/10.1111/ppl.70163","url":null,"abstract":"Triclosan (TCS) is a broad-spectrum antimicrobial disinfectant widely used in pharmaceuticals and personal care products (PPCPs). Due to the extensive usage of PPCPs, TCS inevitably entered the environment and pose harmful effects on the ecosystem. Phytoremediation is an attractive approach to remove TCS from the environment. In this study, a gene encoding the anti-TCS nanobody was transformed into Arabidopsis thaliana (A. thaliana) to enhance the absorption capacity of TCS. Nanobodies are small antibody fragments (ca. 15 kDa) derived from the variable domain of camelid heavy-chain-only antibodies. We constructed two transgenic lines, the T-S-C line with nanobody expression throughout the plant and the T-S-P line with nanobody expression dominant in the roots, were constructed. The expression of nanobodies in A. thaliana alleviated the phytotoxicity of TCS. T-S-C and T-S-P exhibited significantly stronger tolerance to TCS toxicity than the wild type (WT), in either a solid medium system or a hydroponics system. Under the stress of TCS, the seedlings of both transgenic plants exhibited an increase in root length and fresh weight compared to those of WT. Moreover, in the presence of TCS, the activities of superoxide dismutase, peroxidase, catalase, and glutathione in transgenic plants were higher than those in WT. The concentration of TCS absorbed into the T-S-C and T-S-P plants from the solid medium increased by 50.0% and 24.1%, and from the hydroponics system increased by 55.6% and 38.0%, respectively, compared to those absorbed by WT. This study provides a proof of principle that transforming nanobodies into plants represents a novel technology to improve the efficiency of phytoremediation for environmental pollutants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70163"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LvWRKY75 enhances the transcription of LvMYB5 and promotes anthocyanin biosynthesis in lily petals during the blooming phase. LvWRKY75可增强LvMYB5的转录，促进花青素在百合花瓣开花期的生物合成。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70143

Yibo Sun, Xuankun Zhang, Hao Zhang, Minghui Zhang, Shaokun Sun, Wangzhen Han, Xiaojia Zhang, Muhammad Irfan, Lijing Chen, Li Zhang

{"title":"LvWRKY75 enhances the transcription of LvMYB5 and promotes anthocyanin biosynthesis in lily petals during the blooming phase.","authors":"Yibo Sun, Xuankun Zhang, Hao Zhang, Minghui Zhang, Shaokun Sun, Wangzhen Han, Xiaojia Zhang, Muhammad Irfan, Lijing Chen, Li Zhang","doi":"10.1111/ppl.70143","DOIUrl":"https://doi.org/10.1111/ppl.70143","url":null,"abstract":"Anthocyanin accumulation plays a crucial role in enhancing Lilium petal colouration; however, breeding efforts are hindered by our lack of understanding of the complex molecular mechanism behind the pigment's synthesis. This study explores the potential role of the WRKY family gene WRKY75 in anthocyanin synthesis in lilies. Contrary to the inhibitory effect observed in Arabidopsis thaliana, both transient silencing and overexpression analyses of LvWRKY75 indicate that the gene positively regulates anthocyanin synthesis in lilies. The overexpression of LvWRKY75 was found to cause a significant upregulation of structural genes pivotal for anthocyanin biosynthesis in lilies, including Lv3GT, LvDFR and LvANS, as well as the anthocyanin synthesis regulatory gene LvMYB5. Further in-depth analyses, including yeast one-hybrid, electrophoretic mobility shift assay, and dual-luciferase assays, demonstrated that LvWRKY75 binds to the promoter of LvMYB5, enhancing its transcriptional activity. In turn, the increased expression of LvMYB5 upregulates the transcription of downstream genes such as LvDFR and LvANS. In summary, this study provides a deeper understanding of the mechanisms behind anthocyanin synthesis in lilies, contributing to improving molecular breeding strategies for enhancing the flowers' ornamental value and commercial appeal.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70143"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusion of transgene and interspecies hybridization enhances seed yield and root rot disease resistance in Jatropha curcas. 转基因融合和种间杂交提高麻疯树种子产量和根腐病抗性。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70183

Xue Bai, Yiqing Su, Zhonghong Huang, Tong Cheng, Ping Huang, Mingyong Tang

{"title":"Fusion of transgene and interspecies hybridization enhances seed yield and root rot disease resistance in Jatropha curcas.","authors":"Xue Bai, Yiqing Su, Zhonghong Huang, Tong Cheng, Ping Huang, Mingyong Tang","doi":"10.1111/ppl.70183","DOIUrl":"https://doi.org/10.1111/ppl.70183","url":null,"abstract":"Perennial woody plants play an indispensable role in sustainable production. To shorten their juvenile phase and improve health, Jatropha curcas, a classic bioenergy crop, serves as a model for a breeding strategy integrating transgenes with interspecific hybridization. Specifically, overexpressing JcFT (JcFT-OE) J. curcas exhibiting early flowering were crossed with Jatropha integerrima exhibiting bright flowers and robust stems. The resulting hybrids were then backcrossed (BC) with JcFT-OE plants to refine the desired traits. The F1 generation displayed early flowering and an intermediary type of parents, with hard stems, more xylem, lower seed yields, and higher C18:2 content in the seed oil compared to those of wildtype and JcFT-OE transgenic plants. The BC1 generation showed early flowering, diverse shapes of fruit and seeds, and higher seed yield than the F1 generation. Among these lines, BC1-3 produced the highest yield, while the seed yields of J. integerrima, F1 and BC1-1 had notably low yields attributed to fruit dropping. Notably, F1 and BC1 plants demonstrated enhanced resistance to root rot caused by Lasiodiplodia theobromae and lignin contents of stems compared to JcFT-OE, a trait inherited from J. integerrima. Overall, the hybrid plants inherited desirable traits such as precocity and root rot resistance from their parents, resulting in higher seed yields in BC1 individuals. Blending transgenes with hybridization in Jatropha curcas enriches traits, boosting yields and disease resistance in woody plants. Furthermore, FT overexpression has substantial superiority in accelerating the breeding process in woody trees.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70183"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutations of the brassinosteroid biosynthesis gene HvDWARF5 enable balance between semi-dwarfism and maintenance of grain size in barley. 油菜素内酯生物合成基因HvDWARF5的突变使大麦在半矮化和维持籽粒大小之间取得平衡。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70179

Karolina Zolkiewicz, Jana Oklestkova, Beata Chmielewska, Damian Gruszka

{"title":"Mutations of the brassinosteroid biosynthesis gene HvDWARF5 enable balance between semi-dwarfism and maintenance of grain size in barley.","authors":"Karolina Zolkiewicz, Jana Oklestkova, Beata Chmielewska, Damian Gruszka","doi":"10.1111/ppl.70179","DOIUrl":"10.1111/ppl.70179","url":null,"abstract":"Brassinosteroids (BRs) are phytohormones which regulate various developmental processes in plants. They are exceptional phytohormones, as they do not undergo long-distance transport between plant organs. However, knowledge about the function of the enzymes that catalyse BR biosynthesis (particularly its early stages) in cereal crops remains limited. Therefore, this study identifies and analyses the function of the HvDWARF5 (HvDWF5) gene, involved in the early stage of BR biosynthesis in barley (Hordeum vulgare), an important cereal crop, using the TILLING (Targeting Induced Local Lesions IN Genomes) approach. The detailed functional analysis allowed for the identification of various mutations in different gene fragments. The influence of these mutations on plant architecture, reproduction, and yield was characterised. Moreover, effects of the missense and intron retention mutations on sequence and splicing of the HvDWF5 transcript, sequence and predicted structure of the encoded HvDWF5 enzyme, and accumulation of endogenous BR were determined. Some of the barley mutants identified in this study showed semi-dwarfism, a trait of particular importance for cereal breeding and yield. However, unlike other BR mutants in cereals, this did not negatively affect grain size or weight. It indicated that mutations in this gene allow for a balance between plant height reduction and maintenance of grain size. Thus, the results of this study provide a novel insight into the role of the HvDWF5 gene in the BR biosynthesis-dependent regulation of architecture and reproduction of the important cereal crop - barley.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70179"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective bioprocess engineering to enhance omega-6 polyunsaturated fatty acid production from Arthrospira platensis. 有效的生物工艺工程提高了platarthrospira的omega-6多不饱和脂肪酸的产量。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70186

Siddhant Dubey, Reeta Rani Singhania, Praveen Kumar Ramanujam, Chiu-Wen Chen, Cheng-Di Dong, Anil Kumar Patel

{"title":"Effective bioprocess engineering to enhance omega-6 polyunsaturated fatty acid production from Arthrospira platensis.","authors":"Siddhant Dubey, Reeta Rani Singhania, Praveen Kumar Ramanujam, Chiu-Wen Chen, Cheng-Di Dong, Anil Kumar Patel","doi":"10.1111/ppl.70186","DOIUrl":"https://doi.org/10.1111/ppl.70186","url":null,"abstract":"Contrary to the robustness of microbial ω-3 polyunsaturated fatty acid (PUFA) production, the microbial synthesis of ω-6 PUFAs remains challenging. The rising demand for ω-6 PUFAs, especially for pregnancy and infant formulas, calls for scalable and sustainable production methods. Arthrospira platensis, a rarely explored microalgae, shows promise as a platform for producing gamma linoleic acid (GLA) and linolenic acid (LA), key components of ω-6 PUFAs. This study employs a two-phase cultivation approach to enhance ω-6 PUFA production in A. platensis. The initial growth phase was optimized to maximize biomass, followed by a stress-induced phase to boost lipid and ω-6 PUFA accumulation. Notably, ω-6 producing strains like A. platensis are protein-rich and not a high oleaginous species, achieving over 15% total lipid content particularly is significant. Under optimized conditions, a maximum biomass of 4.9 g/L with a productivity rate of (0.233 g/L/day) was obtained at 8 K Lux light irradiance, with 2X nitrogen concentration and 4 mg/L phytohormones. The subsequent stress phase, involving 20 K Lux light, 10 mg/L FeSO4, and 1% glycerol, resulted in a lipid content of 22.8%. This approach led to a 2.4-fold and 1.5-fold increase in microalgal biomass and lipid content respectively. Moreover, C18:2 and C18:3 PUFAs reaching approx. 17.1 ± 0.06% and 24.1 ± 0.07%, respectively. This research promotes microalgae cultivation to meet rising ω-6 PUFA demand, aligning with sustainable development goal 3: Good health and well-being.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70186"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SEEDSTICK Affects Seed Development by Mediating Cytokinin Levels in Cotton. SEEDSTICK 通过调节棉花中的细胞分裂素水平影响种子发育。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70161

Xiaohong Zhang, Yifan Li, Yunjie Ge, Yuhao Mao, Genhai Hu, Qifeng Ma, Eryong Chen

{"title":"SEEDSTICK Affects Seed Development by Mediating Cytokinin Levels in Cotton.","authors":"Xiaohong Zhang, Yifan Li, Yunjie Ge, Yuhao Mao, Genhai Hu, Qifeng Ma, Eryong Chen","doi":"10.1111/ppl.70161","DOIUrl":"https://doi.org/10.1111/ppl.70161","url":null,"abstract":"The SEEDSTICK transcription factor is important for flower and seed development, but the underlying molecular mechanisms remain unclear in cotton. In this study, we identified and cloned two STK homolog genes in upland cotton, an economically valuable cultivated crop. Phylogenetic and sequence analyses showed that the C-terminus of both GhSTKs had a conserved -DJJILHLG amino acid sequence and that GhSTK1 and GhSTK2 were very similar to GaAGL11 and GrAGL11, respectively. Quantitative real-time PCR analysis revealed that both GhSTKs were highly expressed in the ovules, and GUS activity was detected in the style and stigma. Subcellular localization experiments showed that GhSTK1 and GhSTK2 were localized to the nucleus. In Arabidopsis, the overexpression of GhSTK1 or GhSTK2 affected floral organ development and seed formation by increasing the transcript levels of the CKX genes and other genes related to floral development. Silencing both GhSTK1 and GhSTK2 increased the expression of GhFT and GhSHP and led to the earlier appearance of cotton buds. Yeast two-hybrid and bimolecular fluorescence complementation assays indicated that the two GhSTK proteins could interact with the GhSEP3 and GhSEP4 proteins. The present results suggest that GhSTK1 and GhSTK2, which have different sequences and expression patterns, might be functionally redundant and influence the regulation of cotton bud and seed development.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70161"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0