Physiologia plantarum最新文献_第2页

Functional characterisation of WRKY transcription factor CrWRKY48 involved in regulating seed abortion of Ponkan (Citrus reticulata). 参与柑桔种子败育调控的WRKY转录因子CrWRKY48的功能特征

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70048

Liqing Lu, Xiong Gao, Yongjie Qi, Zixian Zha, Zhenghui Gao, Na Ma, Juxun Wu, Haijian Yang, Hualin Yi

{"title":"Functional characterisation of WRKY transcription factor CrWRKY48 involved in regulating seed abortion of Ponkan (Citrus reticulata).","authors":"Liqing Lu, Xiong Gao, Yongjie Qi, Zixian Zha, Zhenghui Gao, Na Ma, Juxun Wu, Haijian Yang, Hualin Yi","doi":"10.1111/ppl.70048","DOIUrl":"https://doi.org/10.1111/ppl.70048","url":null,"abstract":"Citrus fruits are one of the most important fruits in the world, and their seedless character is favored by consumers. WRKY is a plant-specific transcription factor family involved in all aspects of plant growth and development. However, the molecular mechanism of seedless fruit formation in citrus and the role of the WRKY gene family in seed abortion are still poorly understood. In this study, we identified 47 WRKY family genes in the citrus fruit Citrus reticulata and comprehensively characterized the WRKY gene family through gene structure and evolutionary relationships. The expression patterns and protein interaction networks of the WRKY gene family were analyzed based on citrus seed abortion transcriptome data, and several WRKY genes that may be involved in the seed abortion regulation were excavated. Furthermore, CrWRKY48 was verified to regulate seed abortion positively in Arabidopsis thaliana, and the rate of seed abortion caused by overexpression of CrWRKY48 reached 45.48%. Using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling assays, DNA affinity purification sequencing and yeast-one-hybrid assays, we found that CrWRKY48 activated excessive programmed cell death by regulating the expression of programmed cell death-related genes such as SOBIR1. Our results show the potential regulation of the WRKY gene family for citrus seed abortion and provide novel insights into the role of CrWRKY48 in mediating citrus seed abortion by activating programmed cell death.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70048"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the MAP4K gene family reveals GhMAP4K13 regulates drought and salt stress tolerance in cotton. MAP4K基因家族的鉴定揭示了GhMAP4K13调控棉花的抗旱性和耐盐性。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70031

Qing Zeng, Fanjia Peng, Junjuan Wang, Shuai Wang, Xuke Lu, Allah Bakhsh, Yan Li, Bobokhonova Zebinisso Qaraevna, Wuwei Ye, Zujun Yin

{"title":"Identification of the MAP4K gene family reveals GhMAP4K13 regulates drought and salt stress tolerance in cotton.","authors":"Qing Zeng, Fanjia Peng, Junjuan Wang, Shuai Wang, Xuke Lu, Allah Bakhsh, Yan Li, Bobokhonova Zebinisso Qaraevna, Wuwei Ye, Zujun Yin","doi":"10.1111/ppl.70031","DOIUrl":"https://doi.org/10.1111/ppl.70031","url":null,"abstract":"Mitogen-activated protein kinase kinase kinase kinases (MAP4Ks) are a class of highly conserved serine/threonine-protein kinases in eukaryotes. They participate in the typical MAPK cascade system and various signal transduction pathways regulating biological processes in plants, during stressful conditions. To date, genome-wide identification of MAP4Ks in cotton has not been reported. In this study, 77 MAP4K genes were identified in four Gossypium species. Protein characteristics, gene structures, conserved motifs and gene expression analysis were carried out. Genome-wide or fragment duplication has played an important role in the expansion of the GhMAP4K. Promoter cis-acting elements and expression patterns indicated that GhMAP4Ks are related to plant hormones (ABA, MeJA, GA, IAA, SA) and various stresses (drought, hypothermia and wound). Overexpressing GhMAP4K13 in Arabidopsis showed higher stem length in response to drought and salt stress. The wilting degree in virus-induced GhMAP4K13 gene silenced plants was substantially greater than wild type plants under drought and salt stress. Transcriptomic analysis showed that most differentially expressed genes were involved in the MAPK signaling pathway, carbon metabolism and porphyrin metabolism. Additionally, transgenic Arabidopsis and VIGS cotton showed that GhMAP4K13 was positively responsive to drought and salt stresses. This study will play an important role in understanding the function of the MAP4K gene family in response to abiotic stress in cotton.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70031"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated physiological, transcriptomic and metabolomic analyses reveal potential mechanisms of potato tuber dormancy release.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70081

Hao Liu, Hongyang Wang, Youhong Feng, Yan Yang, Cai Feng, Junhua Li, Qamar Ur Zaman, Yunxin Kong, Shah Fahad, Gang Deng

{"title":"Integrated physiological, transcriptomic and metabolomic analyses reveal potential mechanisms of potato tuber dormancy release.","authors":"Hao Liu, Hongyang Wang, Youhong Feng, Yan Yang, Cai Feng, Junhua Li, Qamar Ur Zaman, Yunxin Kong, Shah Fahad, Gang Deng","doi":"10.1111/ppl.70081","DOIUrl":"https://doi.org/10.1111/ppl.70081","url":null,"abstract":"Regulating potato tuber dormancy is crucial for crop productivity and food security. We conducted the first comprehensive physiological, transcriptomic, and metabolomic investigations of two varieties of long and short dormant potato tubers in order to clarify the mechanisms of dormancy release. In the current study, three different dormant stages of UGT (ungerminated tubers), MGT (minimally germinated tubers), and GT (germinated tubers) were obtained by treatment with the germination promoter gibberellin A3 and the germination inhibitor chlorpropham. The results revealed that the contents of reducing reducing sugar, sucrase, glutamine synthetase, and nitrate reductase were increased in the dormancy release stages, whereas the contents of sucrose and starch were decreased, leading to a change in the phenotype of the potato tuber bud eyes. According to transcriptomic and metabolomic investigations, four metabolomic pathways were impacted by the dormancy release process. Zeatin biosynthesis was identified in both potato varieties in the dormant release stage (trans-zeatin riboside, isopentenyl adenosine, 5'-methylthioadenosine, IPT, CYP735A, CKX, and UGT73C); glutathione metabolism was identified in short-dormant potato varieties ((5-L-Glutamyl)-L-amino acid, oxidized glutathione, GPX, IDH1, GGT1_5, and GST); and the pentose phosphate pathway (D-Xylulose 5-phosphate, ribose 1-phosphate, PGD, and RPIA) and the phenylpropanoid biosynthesis (caffeic acid, sinapine, CYP98A, and CSE) were identified in long-dormant potato varieties. In conclusion, the four pathways mentioned above involve DEGs and DEMs that are crucial to the control of tuber dormancy release. This work offers a theoretical foundation and useful recommendations for potato tuber quality improvement and molecular breeding.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70081"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boron controls apical dominance in Pea (Pisum sativum) via promoting polar auxin transport. 硼通过促进植物生长素的极性运输来控制豌豆的顶端优势。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70056

Yutong He, Keren He, Jingwen Mai, Meiyin Ou, Laibin Chen, Yuanyuan Li, Tao Wan, Luping Gu, Sergey Shabala, Xuewen Li, Yalin Li, Min Yu

{"title":"Boron controls apical dominance in Pea (Pisum sativum) via promoting polar auxin transport.","authors":"Yutong He, Keren He, Jingwen Mai, Meiyin Ou, Laibin Chen, Yuanyuan Li, Tao Wan, Luping Gu, Sergey Shabala, Xuewen Li, Yalin Li, Min Yu","doi":"10.1111/ppl.70056","DOIUrl":"https://doi.org/10.1111/ppl.70056","url":null,"abstract":"Plant architecture and subsequent productivity are determined by the shoot apical dominance, which is disturbed by the deficiency of boron, one of the essential trace elements for plant growth and reproduction. However, the mechanism by which B controls shoot apical dominance or axillary bud outgrows under B deficiency is still unclear. This work aimed to investigate the mechanistic basis of this process, with focus on the interaction between B and polar auxin transport. Adopting an all-buds phenotyping methodology and employing several complementary approaches, we found that boron deficiency inhibited plant growth and changed the shoot architecture, resulting in the outgrowth of axillary buds at nodes 1-3. This was related to the auxin accumulation in shoot apical parts buds under B deficiency. Applying N-1-naphthylphthalamic acid to inhibit auxin transport from the shoot apex promoted the outgrowth of axillary buds in boron-sufficient (+B) plants. In decapitated plants, the application of exogenous auxin to the shoot apex only inhibited the outgrowth of axillary buds in +B plants. At higher auxin doses, the toxic effect of IAA was observed in the lower part of the shoot, which was more severe in +B plants than in B-deprived (-B) plants. Furthermore, the expression of PsPIN3 was significantly downregulated under -B conditions. These results indicate that B deficiency inhibits PAT from the apical bud through the main stem to the lower parts, leading to an increase of auxin level in the apical bud, which inhibits the growth of apical buds while stimulating the outgrowth of axillary buds.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70056"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep metabolomics revealed trajectories of jasmonate signaling-mediated primary metabolism in Arabidopsis upon Spodoptera litura herbivory. 深层代谢组学揭示了拟南芥在斜纹夜蛾（Spodoptera litura）草食过程中茉莉酸信号介导的初级代谢轨迹。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70035

Anish Kundu, Paramita Bera, Shruti Mishra, Jyothilakshmi Vadassery

{"title":"Deep metabolomics revealed trajectories of jasmonate signaling-mediated primary metabolism in Arabidopsis upon Spodoptera litura herbivory.","authors":"Anish Kundu, Paramita Bera, Shruti Mishra, Jyothilakshmi Vadassery","doi":"10.1111/ppl.70035","DOIUrl":"https://doi.org/10.1111/ppl.70035","url":null,"abstract":"Plants defend against chewing herbivores by up-regulating jasmonic acid (JA) signaling, which activates downstream signaling cascades and produces numerous secondary metabolites that act as defense molecules against the herbivores. Although secondary metabolism always remains a focus of research, primary metabolism is also reported to be realigned upon herbivory. However, JA signaling-mediated modulation of primary metabolites and their metabolic pathways in plants are mostly unexplored. Here, we applied gas chromatography-mass spectrometry-based untargeted metabolomics aided with computational statistical frameworks on wild type Arabidopsis, mutants of active JA receptor (i.e., CORONATINE-INSENSITIVE 1, COI1-1) and downstream transcription factor (i.e., MYC2) to navigate the JA signaling-mediated primary metabolism alterations during herbivory. Pathway and metabolite's chemical class enrichment analysis revealed JA signaling is crucial for constitutive as well as herbivore-induced primary metabolism and topology of their interaction networks. JA signaling majorly modulated alterations of sugars, amino acids and related metabolites. Herbivory-mediated sugar depletion and induction of methionine for aliphatic glucosinolates are also dependent on JA signaling. Taken together, our results demonstrate trails of JA signaling-mediated primary metabolic alterations associated with herbivory.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70035"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic in vivo monitoring of granum structural changes of Ctenanthe setosa (Roscoe) Eichler during drought stress and subsequent recovery.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.14621

Richard Hembrom, Renáta Ünnep, Éva Sárvári, Gergely Nagy, Katalin Solymosi

{"title":"Dynamic in vivo monitoring of granum structural changes of Ctenanthe setosa (Roscoe) Eichler during drought stress and subsequent recovery.","authors":"Richard Hembrom, Renáta Ünnep, Éva Sárvári, Gergely Nagy, Katalin Solymosi","doi":"10.1111/ppl.14621","DOIUrl":"10.1111/ppl.14621","url":null,"abstract":"Investigating the effects of drought stress and subsequent recovery on the structure and function of chloroplasts is essential to understanding how plants adapt to environmental stressors. We investigated Ctenanthe setosa (Roscoe) Eichler, an ornamental plant that can tolerate prolonged drought periods (40 and 49 days of water withdrawal). Conventional biochemical, biophysical, physiological and (ultra)structural methods combined for the first time in a higher plant with in vivo small-angle neutron scattering (SANS) were used to characterize the alterations induced by drought stress and subsequent recovery. Upon drought stress, no significant changes occurred in the chloroplast ultrastructure, chlorophyll content, 77K fluorescence emission spectra and maximal quantum efficiency of PSII (Qy dark), but the actual quantum efficiency of PSII (Qy light) decreased, the amounts of PSI-LHCII complexes and PSII monomers declined, and that of PSII supercomplexes increased. Thickness of the leaf and of the adaxial hypodermis, chloroplast length and granum repeat distance (RD) values decreased upon drought stress, as shown by light microscopy and SANS, respectively. Because of the very slight (nm-range) changes in RD values, the large biological variability (significant differences in RD values among the leaves and studied leaf regions) and the invasive sampling required for this method, transmission electron microscopy (TEM) hardly showed significant differences. On the other side, in situ SANS analyses provided a unique insight in vivo into the fast structural recovery of the granum structure of drought-stressed leaves, which happened already 18 h after re-watering, while functional and biochemical recovery took place on a longer time scale.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e14621"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of GmLFYa and GmLFYb on Compound Leaf Development in Soybean.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70092

Dongfa Wang, Baolin Zhao, Xuan Zhou, Shaoli Zhou, Liling Yang, Yawen Mao, Quanzi Bai, Weiyue Zhao, Mingzhu Sun, Mingli Liu, Zhijia Gu, Liangliang He, Jianghua Chen

{"title":"Synergistic effects of GmLFYa and GmLFYb on Compound Leaf Development in Soybean.","authors":"Dongfa Wang, Baolin Zhao, Xuan Zhou, Shaoli Zhou, Liling Yang, Yawen Mao, Quanzi Bai, Weiyue Zhao, Mingzhu Sun, Mingli Liu, Zhijia Gu, Liangliang He, Jianghua Chen","doi":"10.1111/ppl.70092","DOIUrl":"https://doi.org/10.1111/ppl.70092","url":null,"abstract":"Legume leaves exhibit diverse compound forms, with various regulatory mechanisms underlying the development. The transcription factor-encoding KNOXI genes are required to promote leaflet initiation in most compound-leafed angiosperms. In non-IRLC (inverted repeat-lacking clade) legumes, KNOXI are expressed in compound leaf primordia but not in others (IRLC). Recent studies have highlighted LFY genes' role in regulating leaflet initiation across legumes. The LFY functions in leaf development are well understood in IRLC legumes but remain unclear in non-IRLC legumes. Soybean, a major crop belonging to non-IRLC legumes, has limited research on the trifoliate leaf morphogenesis. Here, we comprehensively analyzed soybean trifoliate leaf development and characterized two GmLFY gene copies, GmLFYa and GmLFYb, in compound leaf morphogenesis. Analyzing the loss-of-function mutants revealed that Gmlfya displayed a low frequency of simple-like leaves, while the Gmlfyb showed no visible phenotype. However, the Gmlfya Gmlfyb double mutant predominantly displayed simple-like leaves. Additionally, mutations in two genes also affect floral development: each single mutant exhibited slightly deformed floral organs, while double mutant produced inflorescence-like structures. The transformation from floral meristems to inflorescence-like structures is similar to lfy mutant in Arabidopsis but quite different from M. truncatula and L. japonicus. These findings suggest that the two GmLFY genes in soybean collaboratively regulate both compound leaf and flower morphogenesis. Our study not only creates foundational mutant materials for future research on leaf and flower development in soybean but also reinforces the role of LFY orthologs as master regulators in compound leaf morphogenesis across a broader range of legume taxa than previously recognized.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70092"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of an Agrobacterium-mediated transformation system for the genetic engineering of Linum grandiflorum Desf. 农杆菌介导的桔梗基因工程转化体系的建立。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70059

Karol Gad, Hanna Levchuk, Christian Kappel, Michael Lenhard

{"title":"Establishment of an Agrobacterium-mediated transformation system for the genetic engineering of Linum grandiflorum Desf.","authors":"Karol Gad, Hanna Levchuk, Christian Kappel, Michael Lenhard","doi":"10.1111/ppl.70059","DOIUrl":"https://doi.org/10.1111/ppl.70059","url":null,"abstract":"Genetic transformation is a powerful tool in plant biotechnology. However, its application is limited to species that are well-studied and easy to transform. There is a critical need to establish transformation protocols for non-model species. A stable transformation method using Agrobacterium rhizogenes for hairy root transformation and regeneration of transgenic Linum grandiflorum was established. This protocol shows the successful co-transformation of different T-DNA fragments from both the native Ri plasmid and the binary vector with the reporter gene. Hairy roots were produced after inoculation with Agrobacterium rhizogenes from which later shoots were formed from the callus, and subsequently, whole plants were regenerated. This protocol significantly facilitates genomic studies in Linum grandiflorum, particularly in investigating genes at the S-locus supergene, which are crucial for understanding self-incompatibility. Moreover, the established transformation method enables the production of hairy root lines, which can be utilized for the biosynthesis of medically useful and commercially valuable plant metabolites.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70059"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-function relation of cytokinins determines their differential efficiency in mediating tobacco resistance against Pseudomonas syringae. 细胞分裂素的结构-功能关系决定了它们介导烟草对丁香假单胞菌抗性的差异效率。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70028

Dominik K Großkinsky, Eva M Molin, Federico Bosetto, Kerstin Edelsbrunner, Michal Oravec, Kristýna Večeřová, Jan Tříska, Thomas Roitsch

{"title":"Structure-function relation of cytokinins determines their differential efficiency in mediating tobacco resistance against Pseudomonas syringae.","authors":"Dominik K Großkinsky, Eva M Molin, Federico Bosetto, Kerstin Edelsbrunner, Michal Oravec, Kristýna Večeřová, Jan Tříska, Thomas Roitsch","doi":"10.1111/ppl.70028","DOIUrl":"10.1111/ppl.70028","url":null,"abstract":"The classic plant growth-promoting phytohormone cytokinin has been identified and established as a mediator of pathogen resistance in different plant species. However, the resistance effect of structurally different cytokinins appears to vary and may regulate diverse mechanisms to establish resistance. Hence, we comparatively analysed the impact of six different adenine- and phenylurea-type cytokinins on the well-established pathosystem Nicotiana tabacum-Pseudomonas syringae. The efficiency of resistance effects was evaluated based on impacts on the host plant defence response by scoring infection symptoms and the direct impact on the pathogen by assessment of proliferation in planta. To identify common and cytokinin-specific components involved in resistance effects, transcriptome profiling and targeted metabolomics were conducted in leaves treated with the different cytokinins. We observed clearly different potentials of the tested cytokinins in either suppressing infection symptoms or pathogen proliferation. Gene regulation and metabolite analyses revealed cytokinin-type specific impacts on defence components, such as salicylic acid and related signalling, expression of PR proteins, and regulation of specialised metabolism. Cytokinins also strongly affected plant cell physiological parameters, such as a remarkable decrease in amino acid pools. Hence, this study provides comparative information on the efficiency of diverse cytokinins in mediating resistance in one well-studied pathosystem and insights into the specific regulation of resistance effects mediated by different cytokinin molecules. This is particularly relevant for studies on the function of cytokinins or other phytohormones and compounds interacting with cytokinin activities in the context of pathogen infections and other stress scenarios, considering the diverse cytokinins present in plants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70028"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving sulforaphane content in broccoli sprouts by applying Se: transcriptome profiling and coexpression network analysis provide insights into the mechanistic response. 利用Se转录组分析和共表达网络分析提高西兰花芽中萝卜硫素的含量，为其机制响应提供了新的见解。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70037

Shuxiang Mao, Junwei Wang, Zhijun Guo, Huiping Huang, Shengze Wang, Dandan Fei, Juan Liu, Qi Wu, Jin Nie, Qiuyun Wu, Ke Huang

{"title":"Improving sulforaphane content in broccoli sprouts by applying Se: transcriptome profiling and coexpression network analysis provide insights into the mechanistic response.","authors":"Shuxiang Mao, Junwei Wang, Zhijun Guo, Huiping Huang, Shengze Wang, Dandan Fei, Juan Liu, Qi Wu, Jin Nie, Qiuyun Wu, Ke Huang","doi":"10.1111/ppl.70037","DOIUrl":"https://doi.org/10.1111/ppl.70037","url":null,"abstract":"Sulforaphane (SF) is a sulfur (S)-containing isothiocyanate found in cruciferous vegetables and is known for its potent anticancer properties. Broccoli sprouts, in particular, are considered safe and healthy dietary choices due to their high SF content and other beneficial biological activities, such as enhanced metabolite ingestion. The application of selenium (Se) is an excellent approach to enhance the abundance of SF. Previous studies have often focused on gene expression and changes in the synthetic substrates of glucoraphanin (RAA) to explain SF variation in response to Se application. However, the regulatory network and other physiological and biochemical reactions involved in the regulation of SF biosynthesis are poorly understood. In this study, Se-treated broccoli sprouts had higher SF and RAA contents; they increased with increasing Se application. Using RNA-seq in combination with KEGG, GO, phenotypic, and WGCNA analyses, it was observed that not only gene expression was induced but also that glutathione serves as an S donor for SF biosynthesis and acts as an oxidative stress reliever as a result of Se treatment. Additionally, a module related to glucosinolate biosynthesis was identified. Yeast one-hybrid system and dual luciferase reporter assay were utilized. These assays demonstrated the hub transcription factors GATA22, ERF12-like, and MYB108 would directly bind to SUR1 promoter and positively regulate its expression. Our study presents the first global overview of the role of GSH metabolism in response to Se for SF biosynthesis, and provides a novel and valuable gene resource for the molecular breeding of high-SF broccoli.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70037"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0