Physiologia plantarum最新文献

{"title":"Physiological Effect of Thallium in the Facultative Hyperaccumulator Silene latifolia.","authors":"Gaia Regini, Isabella Bettarini, Ilaria Colzi, Emilio Corti, Alessio Papini, Marco Dainelli, Giorgia Guardigli, Antony van der Ent, Nadia Bazihizina, Cristina Gonnelli","doi":"10.1111/ppl.70469","DOIUrl":"https://doi.org/10.1111/ppl.70469","url":null,"abstract":"<p><p>The metallicolous populations of the facultative Tl hyperaccumulator Silene latifolia are extraordinarily tolerant and capable of accumulating up to 80,000 μg Tl g^-1 in nature. A growth stimulatory effect of Tl was observed, and this study set out to determine possible mechanisms. Plants from non-metallicolous and metallicolous populations were subjected to hydroponics dosing experiments at 2.5 and 10 μM Tl. Metal impact on stomatal and non-stomatal photosynthetic constraints, light energy conversion processes and plant anatomy/ultrastructure was assessed over time. Photosynthetic rates improved in 10 μM Tl-treated metallicolous plants by 20% compared to controls, partly due to increased stomatal conductance. The latter was mainly driven by Tl-induced anatomical changes, such as increased central cylinder area and stomatal density, likely to enhance water uptake/translocation and, consequently, leaf metal accumulation. The apparently Tl-favoured CO₂ trafficking resulted in ameliorated maximal photosynthetic capacity. The first signs of photosynthetic declines appeared only at very high Tl leaf concentrations (15,000 μg Tl g^-1), with limitations involving stomatal and biochemical factors; whereas the photochemical reactions remained functional. The observed Tl-induced stimulatory response in growth and net photosynthetic rate in metallicolous plants shows that Tl improves physiological performance in Silene latifolia, mainly through improved stomatal conductance.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70469"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12382316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and Agronomic Insights Into Water Use Efficiency Differences Among Mid-Season Indica Rice Varieties.","authors":"Wenjiang Jing, Xinping Lv, Yu Yan, Jia Yin, Rumeng Sun, Danping Hou, Ying Zhang, Jianhua Zhang, Hao Zhang","doi":"10.1111/ppl.70546","DOIUrl":"https://doi.org/10.1111/ppl.70546","url":null,"abstract":"<p><p>A major challenge in rice (Oryza sativa L.) production is addressing freshwater scarcity without compromising grain yield. Identifying rice varieties with high yield and water use efficiency (WUE) is critical for sustainable agriculture. However, limited information exists regarding the agronomic and physiological traits associated with varying WUE. In this 2-year field study, six mid-season indica rice varieties with different WUE levels were categorized as low (LWVs), medium (MWVs), and high WUE varieties (HWVs). Agronomic and physiological traits were systematically evaluated. The HWVs exhibited significantly higher grain yield and WUE, accompanied by a greater productive tiller percentage, harvest index, and leaf area index (LAI) at key stages. These varieties also showed an increased grain-leaf ratio, flag leaf length and width, specific leaf weight and nitrogen content at heading, and an enhanced net photosynthetic rate during panicle initiation, heading, and mid-grain filling. Additionally, HWVs had higher non-structural carbohydrate (NSC) levels in stems and sheaths, elevated antioxidant enzyme activities (catalase, peroxidase, superoxide dismutase), and superior root biomass, root oxidation activity, and zeatin + zeatin riboside (Z + ZR) contents. Principal component analysis revealed that productive tiller percentage, effective LAI, leaf photosynthesis, root dry weight, and Z + ZR levels in leaves were strongly associated with yield and WUE. These results suggest that enhanced shoot and root traits contribute to high WUE and yield performance. This study provides clear guidance and data support for screening rice varieties suitable for water-limited conditions, contributing to sustainable rice production and food security.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70546"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"S-Nitrosoproteome Analysis of Rice Leaves Highlights the Possible Roles of Superoxide Dismutase in Resistance Against Magnaporthe Oryzae.","authors":"Adeela Munir, Cheol Woo Min, Yiming Wang, Sun Tae Kim, Ravi Gupta","doi":"10.1111/ppl.70515","DOIUrl":"https://doi.org/10.1111/ppl.70515","url":null,"abstract":"<p><p>Nitric oxide (NO) is a key signaling molecule and is known to modulate diverse physiological processes, including defense responses against pathogens. However, the molecular mechanism underlying NO-induced plant immunity remains largely elusive. Here, we investigated the dynamics of NO biosynthesis and its downstream signaling through S-nitrosoproteome analysis of two rice cultivars, Dongjin (DJ, resistant) and Nipponbare (NIP, susceptible), in response to Magnaporthe oryzae, the causative agent of the rice blast disease. M. oryzae inoculation triggered relatively higher nitric oxide synthase (NOS/NOA1)-mediated NO biosynthesis in DJ than that of NIP. High-throughput, site-specific S-nitrosoproteome analysis using the iodoTMT-based mass spectrometry approach led to the identification of 511 S-nitrosated peptides corresponding to 335 proteins, representing the most comprehensive set of S-nitrosated peptides identified in rice so far. In particular, the S-nitrosated site intensity of superoxide dismutase (SOD) at Cysteine²⁶³ was significantly reduced specifically in DJ in response to pathogen inoculation. We observed that in vitro S-nitrosation of SOD enhances its activity, and its M. oryzae infection-triggered denitrosation was correlated with the S-nitrosoglutathione reductase (GSNOR) activation. This denitrosation-mediated suppression of SOD activity likely leads to the accumulation of superoxide ions during infection, which triggers immune responses. Altogether, our results suggest that NO-mediated S-nitrosation of SOD plays a crucial role in orchestrating redox-dependent defense signaling, which likely contributes to the contrasting resistance responses observed in the two cultivars. These findings provide novel insights into the functional implications of S-nitrosation in plant immunity and highlight redox-regulatory proteins as key targets of NO signaling during pathogen challenge.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70515"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CsMADS Negatively Regulates the Theobromine Content of Leaves in Tea Plants.","authors":"Lingxiao Duan, Hanmo Fang, Jianqiang Ma, Pengcheng Yuan, Jiqiang Jin, Weizhong He, Haoran Liu, Liang Chen","doi":"10.1111/ppl.70479","DOIUrl":"https://doi.org/10.1111/ppl.70479","url":null,"abstract":"<p><p>Theobromine is a significant purine alkaloid found in tea plants, known for its various bioactive functions in humans. While the metabolic pathway for purine alkaloids, particularly involving the methyltransferase CsTCS1, has been largely understood, information regarding the upstream regulatory network of theobromine remains limited. In this study, we collected transcriptome data from tea plants exhibiting different levels of purine alkaloids and applied weighted gene co-expression network analysis (WGCNA) to identify the upstream regulatory network modules associated with each purine alkaloid. The module labeled MEred was discovered as one of the negative regulatory network modules affecting theobromine, with the gene CsMADS identified as a hub gene within this module. To investigate the role of CsMADS in theobromine accumulation, we examined the concentrations of purine alkaloids and the expression patterns of related genes in the CsMADS-silenced tea plants. Results showed that theobromine levels increased in the tender leaves of CsMADS-silenced tea plants, while the expression level of CsTCS1 decreased in these plants. Meanwhile, CsMADS did not influence mechanical harvesting-related traits such as leaf droopiness or leaf tip expansion. In summary, CsMADS negatively regulates the theobromine content in tea leaves without adversely affecting mechanical harvesting capabilities.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70479"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strigolactone and Hydrogen Sulfide Regulate Carbohydrate Metabolism and Ion Homeostasis Through H⁺-ATPase Activity and K⁺ Retention Under Salt Stress.","authors":"M Nasir Khan, Manzer H Siddiqui, Francisco J Corpas, Zahid Hameed Siddiqui, Mazen A AlSolami, Khalaf M Alhussaen, Abdulaziz A H Alsumary","doi":"10.1111/ppl.70475","DOIUrl":"https://doi.org/10.1111/ppl.70475","url":null,"abstract":"<p><p>This study investigates the roles of strigolactones (SL) and endogenous hydrogen sulfide (H₂S) in regulating physiological processes in tomato seedlings under NaCl-induced stress. Exposure of the seedlings to 100 mM NaCl stress reduced K⁺ content by 21% while increasing Na⁺ accumulation by 69%, disrupting the K⁺/Na⁺ ratio and impairing H⁺-ATPase activity. However, the application of SL improved H⁺-ATPase activity and K⁺ uptake and reduced Na⁺ accumulation. However, the application of 1 μM dl-propargylglycine (PAG; an H₂S biosynthesis inhibitor) negated these positive effects of SL, suggesting that H₂S plays a crucial role in SL-mediated ion homeostasis. NaCl stress also elevated the levels of reactive oxygen species, which were significantly reduced upon SL treatment. On the other hand, the application of PAG reversed these effects, confirming the involvement of H₂S in mitigating oxidative stress. Moreover, SL modulated carbohydrate metabolism by promoting starch accumulation and enhancing the activity of key enzymes such as sucrose synthase and soluble acid invertase. This process helps maintain osmoprotection and energy balance under stress conditions. However, these effects were abolished by H₂S biosynthesis inhibitor PAG, indicating its critical role in SL-mediated sugar metabolism. Overall, the results indicate that SL mitigates NaCl-induced stress by regulating H⁺-ATPase activity, maintaining ion homeostasis, reducing oxidative damage, and regulating carbohydrate metabolism via H₂S-dependent mechanisms. These findings highlight the potential of SL and H₂S to improve plant tolerance to NaCl stress.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70475"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defense Responses of Native Plant Communities to Alien Plant Invasion.","authors":"Zhaoqi Zhu, Miaomiao Cui, Haochen Yu, Xue Fan, Xuanwen Wu, Shoujiang Liu, Zhicong Dai, Guangqian Ren, Daolin Du","doi":"10.1111/ppl.70492","DOIUrl":"10.1111/ppl.70492","url":null,"abstract":"<p><p>Understanding the mechanisms that facilitate the successful invasion of invasive plant species is essential for improving invasive species management and ensuring biosecurity. Traditional invasion ecology has primarily focused on the traits of invasive plants or the static characteristics of invaded communities, while comparatively overlooking the potential active responses of native plant communities during the invasion process. Drawing inspiration from the concept of \"herd immunity\" in medicine, this study proposes that native plant communities may develop a \"community defense\" response mechanism through a series of ecological defense processes. This article begins by reviewing the two principal pathways that trigger plant defense: pathogens and environmental changes, along with the mechanisms behind them. We then discuss the impact of plant defense responses on the invasiveness of alien species and the invasibility of native plant communities. Building on this, we explore four key mechanisms through which invasive plants may induce defense responses in native species: pathogens and herbivores, allelopathic substances, interspecific competition, and environmental conditions. Based on these insights, we propose \"Plant community defense hypothesis,\" which posits that plant invasions can trigger defense responses in native plant communities that, in turn, influence community invasibility. Finally, the article provides an experimental design to test this hypothesis. This review highlights the understanding of dynamic responses in plant communities and introduces a novel theoretical pathway to explain invasion heterogeneity at the community level. It enriches invasion ecology and offers theoretical support for more targeted management of alien plants.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70492"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought-Induced Coordination of Photosynthesis, Stomata, and Hydraulics in Maize Leaves Between Sensitive and Tolerant Varieties.","authors":"Hao Li, Yanqun Zhang, Kaixuan Du, Xinlong Hu, Yan Mo, Di Xu, Shuji Wang, Baozhong Zhang","doi":"10.1111/ppl.70487","DOIUrl":"https://doi.org/10.1111/ppl.70487","url":null,"abstract":"<p><p>Identification of drought-tolerant maize varieties in the context of climate change is critical. Although many studies have reported that the coordination of stomatal and hydraulic conductance of plant leaves ensures the net photosynthetic rate, it is unclear whether the arrangement of these three parameters is consistent among maize varieties differing in drought tolerance. Therefore, in this study, gas exchange parameters, hydraulic properties, and stomatal structure of leaves from eight maize varieties under full and deficit irrigation (DI) were determined. Drought tolerance of varieties was assessed using principal component analysis, and the coordination of photosynthesis, stomatal and hydraulic conductance, as well as stomatal behavior was analyzed between drought-sensitive (DSVs) and drought-tolerant varieties (DTVs). Eight maize varieties were categorized into DSVs and DTVs based on the evaluation of these agronomic and physiological parameters. Significant variety-specific responses of physiological parameters to DI were found, with at least one parameter being significantly affected in each variety. Leaf net photosynthesis rate and stomatal conductance showed a tight coordination with hydraulic conductance among DSVs; however, this coordination was potentially absent among DTVs. Simulations of stomatal behavior based on Ball-Berry and Medlyn models showed that DI significantly reduced the model sensitivity parameters of m and g₁ regardless of DSVs and DTVs. The study highlights the importance of physiological trait coordination in drought responses. The coordination between stomatal and hydraulic traits may be absent in DTVs, implying a potentially flexible adaptation strategy that could be exploited to improve maize drought tolerance.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70487"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Modulation of Leaf Cell-Wall Composition in Arabidopsis thaliana by the Drought-Mitigating Rhizobacterium, Bacillus endophyticus J13.","authors":"Raunak Sharma, Lavi Rastogi, Prashant Anupama-Mohan Pawar, P T Nikhil, Sridev Mohapatra","doi":"10.1111/ppl.70498","DOIUrl":"https://doi.org/10.1111/ppl.70498","url":null,"abstract":"<p><p>Plant growth-promoting rhizobacteria (PGPR) are beneficial soil bacteria that reside near plant roots (in the rhizosphere) and support plants in various ways. The specific molecular mechanisms involved in these beneficial interactions are still under scrutiny. In this context, the present study describes the role of Bacillus endophyticus J13, a multiple abiotic-stress-tolerant PGPR, in modulating various components of the leaf cell wall in Arabidopsis thaliana, under well-watered and drought conditions. We have previously reported the positive impact of J13 on drought mitigation in A. thaliana by modulation of soil water content. In the present study, we inoculated A. thaliana roots with J13 under well-watered or water-stressed conditions and analysed the cell wall composition in leaves under the different treatments. We observed an inoculation-specific reduction in lignin content, both under well-watered and water-stressed conditions, while the cellulose content was found to be significantly higher in water-stressed plants inoculated with J13. The expression of genes involved in both the lignin and cellulose biosynthetic pathways was upregulated in water-stressed plants inoculated with J13. J13 inoculation in A. thaliana mutants compromised in cellulose biosynthesis eliminated the beneficial impact of the bacteria, indicating the importance of a functional cellulose biosynthetic pathway in J13-mediated drought tolerance in A. thaliana. J13 inoculation in A. thaliana mutants with altered lignin composition (mutants with higher S lignin) exhibits no beneficial impact by J13, demonstrating the importance of lignin composition towards the plant-growth-promoting activity of J13 in A. thaliana. This study demonstrates that J13 enhances the saccharification efficiency of A. thaliana leaves under dehydration stress by reducing the amount of lignin and elevating the amount of cellulose. Overall, our study suggested that modulation in cell wall composition could be an important mechanism employed by J13 for drought mitigation in A. thaliana, which also positively influences polysaccharide digestibility. Therefore, such a strategy may be used to improve plant immunity and lignocellulosic properties.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70498"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of Transcription Factors, Photosynthesis, and Nitrogen Metabolic Genes Modulates Nitrogen Stress with Abscisic Acid in Rapeseed.","authors":"Atif Ayub, Airish Nayab, Nan Yunyou, Xie Yuyu, Shi Derong, Maqsood Ul Hussan, Sadam Hussain, Muhammad Farooq, Tian Hui, Hui Jing, Gao Yajun","doi":"10.1111/ppl.70486","DOIUrl":"https://doi.org/10.1111/ppl.70486","url":null,"abstract":"<p><p>Nitrogen (N) is essential for plant growth, but excessive fertilizer use decreases nitrogen use efficiency (NUE) and raises environmental concerns. This study investigated the effect of exogenous abscisic acid (ABA; 50 μM) application on rapeseed (Brassica napus L.) plants under hydroponic conditions with high (7.5 mM NO₃ ^-) and low (0.25 mM NO₃ ^-) nitrate. N deficiency significantly reduced growth, chlorophyll, gas exchange, and stomatal size (length and width). Exogenous ABA application under low N conditions increased the N content (23.1%) and the activities of nitrate reductase (NR; 15.7%), glutamine synthetase (GS; 30.3%), and GS/glutamate synthase (GOGAT; 19.3%). It also enhanced activities of antioxidant enzymes: superoxide dismutase (SOD; 18.3%), peroxidase (POD; 30.2%), ascorbate peroxidase (APX; 39%), catalase (CAT; 16.3%), and endogenous hormones: ABA (35.3%), salicylic acid (SA; 20.5%), indole acetic acid (IAA; 49.8%), and jasmonic acid (JA; 90.3%) compared to untreated low N conditions. Histochemical staining (NBT and DAB) confirmed that ABA alleviated oxidative stress under N deficiency. Transcriptomic analysis identified differentially expressed genes related to photosynthesis, antioxidant defense, N metabolism, and ABA signaling (PYL/PYR/RCAR receptors, PP2C phosphatases, SnRK2 kinases). Transcription factors from bZIP, MYB, and AP2/ERF families were significantly regulated. These results highlight the mechanisms of ABA-mediated N stress mitigation and provide a basis for improving NUE in rapeseed through genetic approaches such as overexpression CRISPR/Cas9, supporting sustainable agriculture.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70486"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Lab to Field: Harnessing H₂O₂-Mediated Upregulation of Plant Capacities Under Abiotic Stresses.","authors":"Hassan Iqbal, Chen Yaning, Syed Turab Raza, Sona Karim, Muhammad Shareef, Muhammad Waqas","doi":"10.1111/ppl.70488","DOIUrl":"https://doi.org/10.1111/ppl.70488","url":null,"abstract":"<p><p>Climate-driven abiotic stresses, responsible for approximately 50% of global crop yield losses, are putting agriculture under increasing pressure, demanding smarter ways to strengthen plants' natural defenses beyond genetic modification. Hydrogen peroxide (H₂O₂), long recognized as a key signaling molecule, plays a powerful role in helping plants cope with environmental stress. This review deciphers the mechanistic basis of H₂O₂-mediated capacity enhancement under diverse stresses (drought, salinity, heavy metals, heat, cold) while also addressing climate-intensified challenges like waterlogging and ultraviolet (UV) radiation. We spotlight its roles in energy partitioning, hormonal signaling, asset optimization, and internal supply chain dynamics, positioning H₂O₂ as a multifunctional coordinator of stress resilience. Moving beyond the antioxidant narrative, this review highlights the active role of H₂O₂ in reorganizing plant responses to real-world stress. Can a molecule once known only for causing damage now lead the next wave of environmentally friendly, stress-resilient agriculture? We propose that H₂O₂-based strategies represent a promising shift toward redox-guided, non-genetic interventions bridging laboratory research with practical field applications and opening new pathways for resilient crop management.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70488"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}