Physiologia plantarum最新文献_第2页

Reactive carbonyl species function downstream of reactive oxygen species in chitosan-induced stomatal closure.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70094

Israt Jahan, Md Moshiul Islam, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Jun'ichi Mano, Yoshiyuki Murata

{"title":"Reactive carbonyl species function downstream of reactive oxygen species in chitosan-induced stomatal closure.","authors":"Israt Jahan, Md Moshiul Islam, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Jun'ichi Mano, Yoshiyuki Murata","doi":"10.1111/ppl.70094","DOIUrl":"10.1111/ppl.70094","url":null,"abstract":"An elicitor, chitosan (CHT), induces stomatal closure in plants, which is accompanied by salicylhydroxamic acid (SHAM)-sensitive peroxidases-mediated reactive oxygen species (ROS) production in guard cells. Reactive carbonyl species (RCS) function downstream of ROS in abscisic acid (ABA) and methyl jasmonate (MeJA) signalling in guard cells. However, the involvement of RCS in CHT-induced stomatal closure is still unknown. In this study, we used transgenic tobacco (Nicotiana tabacum) plants overexpressing Arabidopsis thaliana 2-alkenal reductase (AER-OE tobacco) and Arabidopsis wild-type (WT) plants to investigate whether RCS is involved in CHT-induced stomatal closure. Chitosan-induced stomatal closure was inhibited in the tobacco AER-OE plants. In the WT tobacco and Arabidopsis plants, CHT-induced stomatal closure was inhibited by RCS scavengers, carnosine and pyridoxamine. Chitosan significantly increased RCS production in the WT tobacco and Arabidopsis, but in the tobacco AER-OE plants, chitosan did not increase significantly RCS accumulation. Moreover, neither the application of RCS scavengers to both WT plants nor scavenging RCS by AER-OE affected the CHT-induced ROS accumulation. However, treatment with a peroxidase inhibitor, SHAM, significantly inhibited CHT-induced RCS accumulation in WT tobacco and Arabidopsis plants. Taken together, these results suggest that RCS acts downstream of ROS production in CHT signalling in guard cells of A. thaliana and N. tabacum.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70094"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substitutability evaluation and resource utilization of non-medicinal parts of Ardisia crenata Sims, a traditional ethnic medicine.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70068

Jie Peng, Sha Liu, Fusong Liu, Lin Qin, Qian Wang, Yuqi He, Delin Zhang, Wenping He, Xinyue Ke, Faming Wu, Xiaoshuang Wang

{"title":"Substitutability evaluation and resource utilization of non-medicinal parts of Ardisia crenata Sims, a traditional ethnic medicine.","authors":"Jie Peng, Sha Liu, Fusong Liu, Lin Qin, Qian Wang, Yuqi He, Delin Zhang, Wenping He, Xinyue Ke, Faming Wu, Xiaoshuang Wang","doi":"10.1111/ppl.70068","DOIUrl":"https://doi.org/10.1111/ppl.70068","url":null,"abstract":"The severe scarcity of wild resources of Ardisia crenata Sims (AS) has greatly limited its current clinical and pharmaceutical applications. We aimed to demonstrate the substitutability of stems and leaves for AS roots. In this study, 28 local samples from the Guizhou Province of China were selected for a comprehensive comparison of quality markers, chemical composition and efficacy. HPLC analysis showed that both stems and leaves contained bergenin, the main active ingredient of AS. Metabolomics studies showed that the chemical composition of the three parts was 99% similar. Network pharmacology analysis showed that similar signalling targets including STAT3 and MAPK1, AKT1 and EP300, SRC and TP53, were targeted by three parts. Animal experiments further demonstrated that extracts from the three different parts of the plant relieved the pathological symptoms of acute pharyngitis, and decreased serum inflammatory factors, showing similar efficacy. In sum, these findings strongly proved that stems and leaves can be used as supplements or substitutes for roots, providing a powerful strategy to solve the problem of AS resource shortage and greatly promoting the optimal utilization of AS. The study also anticipated some additional benefits, including the expansion of natural sources of bergenin and the discovery of potential cancer-related targets of AS.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70068"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BBX22 enhances the accumulation of antioxidants to inhibit DNA damage and promotes DNA repair under high UV-B. BBX22增强抗氧化剂的积累，抑制DNA损伤，促进高UV-B下的DNA修复。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70038

Nikhil Job, Shubhi Dwivedi, Maneesh Lingwan, Sourav Datta

{"title":"BBX22 enhances the accumulation of antioxidants to inhibit DNA damage and promotes DNA repair under high UV-B.","authors":"Nikhil Job, Shubhi Dwivedi, Maneesh Lingwan, Sourav Datta","doi":"10.1111/ppl.70038","DOIUrl":"https://doi.org/10.1111/ppl.70038","url":null,"abstract":"Under changing climatic conditions, plant exposure to high-intensity UV-B can be a potential threat to plant health and all plant-derived human requirements, including food. It's crucial to understand how plants respond to high UV-B radiation so that proper measures can be taken to enhance tolerance towards high UV-B stress. We found that BBX22, a B-box protein-coding gene, is strongly induced within one hour of exposure to high-intensity UV-B. Our metabolomics data indicated that BBX22 promotes the accumulation of antioxidants like ascorbic acid and proline. These antioxidants play a vital role in shielding plants exposed to high UV-B from the detrimental effects of Reactive Oxygen Species (ROS), including DNA damage. Additionally, BBX22 promotes DNA damage repair by inducing the expression of DNA repair genes like UVR1 and UVR3. BBX22 directly binds to the promoter of UVR1 to regulate its expression. Furthermore, BBX22 indirectly induces the expression of UVR1 and UVR3 by enhancing the binding of HY5 to their promoters. Together, these results suggest a multi-pronged role of BBX22 in protection against high-intensity UV-B. Enhancing BBX22 levels or its orthologs in different plant species can potentially offer DNA damage protection and tolerance against intense UV radiation.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70038"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell size has pervasive effects on the functional composition and morphology of leaves: a case study in Rhododendron (Ericaceae). 细胞大小对叶片的功能组成和形态具有普遍影响：杜鹃花（杜鹃花科）案例研究。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70054

Arezoo Dastpak, Monica Williams, Sally Perkins, John A Perkins, Charles Horn, Patrick Thompson, Connor Ryan, Juliana Medeiros, Yi-Dong An, Guo-Feng Jiang, Kevin A Simonin, Adam B Roddy

{"title":"Cell size has pervasive effects on the functional composition and morphology of leaves: a case study in Rhododendron (Ericaceae).","authors":"Arezoo Dastpak, Monica Williams, Sally Perkins, John A Perkins, Charles Horn, Patrick Thompson, Connor Ryan, Juliana Medeiros, Yi-Dong An, Guo-Feng Jiang, Kevin A Simonin, Adam B Roddy","doi":"10.1111/ppl.70054","DOIUrl":"https://doi.org/10.1111/ppl.70054","url":null,"abstract":"The leaf economics spectrum (LES) characterizes a tradeoff between building a leaf for durability versus for energy capture and gas exchange, with allocation to leaf dry mass per projected surface area (LMA) being a key trait underlying this tradeoff. However, regardless of the biomass supporting the leaf, high rates of gas exchange are typically accomplished by small, densely packed stomata on the leaf surface, which is enabled by smaller genome sizes. Here, we investigate how variation in genome size-cell size allometry interacts with variation in biomass allocation (i.e. LMA) to influence the maximum surface conductance to CO2 and the rate of resource turnover as measured by leaf water residence time. We sampled both evergreen and deciduous Rhododendron (Ericaceae) taxa from wild populations and botanical gardens, including naturally occurring putative hybrids and artificially generated hybrids. We measured genome size, anatomical traits related to cell sizes, and morphological traits related to water content and dry mass allocation. Consistent with the LES, higher LMA was associated with slower water residence times, and LMA was strongly associated with leaf thickness. Although anatomical and morphological traits varied orthogonally to each other, cell size had a pervasive impact on leaf functional anatomy: for a given leaf thickness, reducing cell size elevated the leaf surface conductance and shortened the mean water residence time. These analyses clarify how anatomical traits related to genome size-cell size allometry can influence leaf function independently of morphological traits related to leaf longevity and durability.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70054"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterisation of WRKY transcription factor CrWRKY48 involved in regulating seed abortion of Ponkan (Citrus reticulata). 参与柑桔种子败育调控的WRKY转录因子CrWRKY48的功能特征

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70048

Liqing Lu, Xiong Gao, Yongjie Qi, Zixian Zha, Zhenghui Gao, Na Ma, Juxun Wu, Haijian Yang, Hualin Yi

{"title":"Functional characterisation of WRKY transcription factor CrWRKY48 involved in regulating seed abortion of Ponkan (Citrus reticulata).","authors":"Liqing Lu, Xiong Gao, Yongjie Qi, Zixian Zha, Zhenghui Gao, Na Ma, Juxun Wu, Haijian Yang, Hualin Yi","doi":"10.1111/ppl.70048","DOIUrl":"https://doi.org/10.1111/ppl.70048","url":null,"abstract":"Citrus fruits are one of the most important fruits in the world, and their seedless character is favored by consumers. WRKY is a plant-specific transcription factor family involved in all aspects of plant growth and development. However, the molecular mechanism of seedless fruit formation in citrus and the role of the WRKY gene family in seed abortion are still poorly understood. In this study, we identified 47 WRKY family genes in the citrus fruit Citrus reticulata and comprehensively characterized the WRKY gene family through gene structure and evolutionary relationships. The expression patterns and protein interaction networks of the WRKY gene family were analyzed based on citrus seed abortion transcriptome data, and several WRKY genes that may be involved in the seed abortion regulation were excavated. Furthermore, CrWRKY48 was verified to regulate seed abortion positively in Arabidopsis thaliana, and the rate of seed abortion caused by overexpression of CrWRKY48 reached 45.48%. Using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling assays, DNA affinity purification sequencing and yeast-one-hybrid assays, we found that CrWRKY48 activated excessive programmed cell death by regulating the expression of programmed cell death-related genes such as SOBIR1. Our results show the potential regulation of the WRKY gene family for citrus seed abortion and provide novel insights into the role of CrWRKY48 in mediating citrus seed abortion by activating programmed cell death.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70048"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the MAP4K gene family reveals GhMAP4K13 regulates drought and salt stress tolerance in cotton. MAP4K基因家族的鉴定揭示了GhMAP4K13调控棉花的抗旱性和耐盐性。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70031

Qing Zeng, Fanjia Peng, Junjuan Wang, Shuai Wang, Xuke Lu, Allah Bakhsh, Yan Li, Bobokhonova Zebinisso Qaraevna, Wuwei Ye, Zujun Yin

{"title":"Identification of the MAP4K gene family reveals GhMAP4K13 regulates drought and salt stress tolerance in cotton.","authors":"Qing Zeng, Fanjia Peng, Junjuan Wang, Shuai Wang, Xuke Lu, Allah Bakhsh, Yan Li, Bobokhonova Zebinisso Qaraevna, Wuwei Ye, Zujun Yin","doi":"10.1111/ppl.70031","DOIUrl":"https://doi.org/10.1111/ppl.70031","url":null,"abstract":"Mitogen-activated protein kinase kinase kinase kinases (MAP4Ks) are a class of highly conserved serine/threonine-protein kinases in eukaryotes. They participate in the typical MAPK cascade system and various signal transduction pathways regulating biological processes in plants, during stressful conditions. To date, genome-wide identification of MAP4Ks in cotton has not been reported. In this study, 77 MAP4K genes were identified in four Gossypium species. Protein characteristics, gene structures, conserved motifs and gene expression analysis were carried out. Genome-wide or fragment duplication has played an important role in the expansion of the GhMAP4K. Promoter cis-acting elements and expression patterns indicated that GhMAP4Ks are related to plant hormones (ABA, MeJA, GA, IAA, SA) and various stresses (drought, hypothermia and wound). Overexpressing GhMAP4K13 in Arabidopsis showed higher stem length in response to drought and salt stress. The wilting degree in virus-induced GhMAP4K13 gene silenced plants was substantially greater than wild type plants under drought and salt stress. Transcriptomic analysis showed that most differentially expressed genes were involved in the MAPK signaling pathway, carbon metabolism and porphyrin metabolism. Additionally, transgenic Arabidopsis and VIGS cotton showed that GhMAP4K13 was positively responsive to drought and salt stresses. This study will play an important role in understanding the function of the MAP4K gene family in response to abiotic stress in cotton.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70031"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated physiological, transcriptomic and metabolomic analyses reveal potential mechanisms of potato tuber dormancy release.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70081

Hao Liu, Hongyang Wang, Youhong Feng, Yan Yang, Cai Feng, Junhua Li, Qamar Ur Zaman, Yunxin Kong, Shah Fahad, Gang Deng

{"title":"Integrated physiological, transcriptomic and metabolomic analyses reveal potential mechanisms of potato tuber dormancy release.","authors":"Hao Liu, Hongyang Wang, Youhong Feng, Yan Yang, Cai Feng, Junhua Li, Qamar Ur Zaman, Yunxin Kong, Shah Fahad, Gang Deng","doi":"10.1111/ppl.70081","DOIUrl":"https://doi.org/10.1111/ppl.70081","url":null,"abstract":"Regulating potato tuber dormancy is crucial for crop productivity and food security. We conducted the first comprehensive physiological, transcriptomic, and metabolomic investigations of two varieties of long and short dormant potato tubers in order to clarify the mechanisms of dormancy release. In the current study, three different dormant stages of UGT (ungerminated tubers), MGT (minimally germinated tubers), and GT (germinated tubers) were obtained by treatment with the germination promoter gibberellin A3 and the germination inhibitor chlorpropham. The results revealed that the contents of reducing reducing sugar, sucrase, glutamine synthetase, and nitrate reductase were increased in the dormancy release stages, whereas the contents of sucrose and starch were decreased, leading to a change in the phenotype of the potato tuber bud eyes. According to transcriptomic and metabolomic investigations, four metabolomic pathways were impacted by the dormancy release process. Zeatin biosynthesis was identified in both potato varieties in the dormant release stage (trans-zeatin riboside, isopentenyl adenosine, 5'-methylthioadenosine, IPT, CYP735A, CKX, and UGT73C); glutathione metabolism was identified in short-dormant potato varieties ((5-L-Glutamyl)-L-amino acid, oxidized glutathione, GPX, IDH1, GGT1_5, and GST); and the pentose phosphate pathway (D-Xylulose 5-phosphate, ribose 1-phosphate, PGD, and RPIA) and the phenylpropanoid biosynthesis (caffeic acid, sinapine, CYP98A, and CSE) were identified in long-dormant potato varieties. In conclusion, the four pathways mentioned above involve DEGs and DEMs that are crucial to the control of tuber dormancy release. This work offers a theoretical foundation and useful recommendations for potato tuber quality improvement and molecular breeding.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70081"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectral unmixing of hyperspectral images revealed pine wilt disease sensitive endmembers.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70090

Seok Won Jeong, Il Hwan Lee, Yang-Gil Kim, Kyu-Suk Kang, Donghwan Shim, Vaughan Hurry, Alexander G Ivanov, Youn-Il Park

{"title":"Spectral unmixing of hyperspectral images revealed pine wilt disease sensitive endmembers.","authors":"Seok Won Jeong, Il Hwan Lee, Yang-Gil Kim, Kyu-Suk Kang, Donghwan Shim, Vaughan Hurry, Alexander G Ivanov, Youn-Il Park","doi":"10.1111/ppl.70090","DOIUrl":"https://doi.org/10.1111/ppl.70090","url":null,"abstract":"Throughout the entire cycle of leaf phenological events, leaf colour undergoes changes that are influenced by either abiotic stress or biotic infection. These changes in colouration are closely linked to the quantity and quality of photosynthetic pigments, which directly impact the primary productivity of plants. Therefore, monitoring and quantifying leaf colouration changes are crucial for distinguishing damage caused by pine wilt nematodes from natural tree senescence. In this study, a hyperspectral camera sensor was employed for the non-invasive and non-destructive evaluation of needle colour changes in coniferous trees grown in field tests. Three distinct needle colour variations of six coniferous tree species were selected and monitored using a hyperspectral sensor: those displaying seasonal autumn colours, undergoing nematode-infected necrosis processes, and experiencing natural death. To mitigate the inherently mixed spectral properties of hyperspectral data, endmembers were extracted from individual images using the Purity Pixel Index algorithm under the assumption of linear mixing of endmembers. From a total of 1,321 endmembers extracted from 378 hyperspectral images of six pine species, eight endmembers were ultimately chosen to reconstruct hyperspectral images and generate abundance maps. Among these eight endmembers, four represent varying levels of photosynthetic pigment contents-ranging from very low to high. Consequently, these coniferous endmembers hold promise for assessing seasonal leaf phenology and the extent of damage in pine trees infected by pine wilt nematodes. This comprehensive approach underscores the effectiveness of spectral unmixing of hyperspectral images in advancing precision forestry through meticulous coniferous needle trait analysis.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70090"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bicarbonate use reduces the photorespiration in Ottelia alismoides adapting to the CO₂-fluctuated aquatic systems.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70085

Pengpeng Li, Zuying Liao, Bo Zhang, Liyan Yin, Wei Li, Hong Sheng Jiang

{"title":"Bicarbonate use reduces the photorespiration in Ottelia alismoides adapting to the CO2-fluctuated aquatic systems.","authors":"Pengpeng Li, Zuying Liao, Bo Zhang, Liyan Yin, Wei Li, Hong Sheng Jiang","doi":"10.1111/ppl.70085","DOIUrl":"https://doi.org/10.1111/ppl.70085","url":null,"abstract":"Underwater CO2 concentration fluctuates extremely in natural water bodies. Under low CO2, the unique CO2 concentrating mechanism in aquatic plants, bicarbonate use, can suppress photorespiration. However, it remains unknown (1) to what extent bicarbonate use reduces photorespiration, (2) how exactly photorespiration varies between bicarbonate-users and CO2-obligate users under CO2-fluctuated environments, and (3) what are differences in Rubisco characteristics between these two types of aquatic plants. In the present study, the bicarbonate user Ottelia alismoides and its phylogenetically close CO2-obligate user Blyxa japonica were chosen to answer these questions. The results showed that bicarbonate use saved ~13% carbon loss under low CO2 via decreasing photorespiration in O. alismoides. Through bicarbonate use, the photorespiration of O. alismoides was kept stable both under high and low underwater CO2 concentrations, while the photorespiration significantly increased in the CO2-obligate user B. japonica under low CO2. However, B. japonica showed a significantly higher photosynthesis rate than O. alsimoides when CO2 was sufficient. These differences could be related to the kinetic characteristics of Rubisco showing a higher carboxylation turnover rate (Kcat) in B. japonica, and the similar affinity to CO2 (Kc) and specificity factor (Sc/o) in these two species that might be determined by the variation of six amino acid residuals in Rubisco large subunit sequences, especially the site 281 (A vs. S) and 282 (H vs. F). All these differences in photorespiration and kinetic characteristics of Rubisco could explain the distribution patterns of bicarbonate users and CO2-obligate users in the field.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70085"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The promotive and repressive effects of exogenous H₂O₂ on Arabidopsis seed germination and seedling establishment depend on application dose.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70098

Yakong Wang, Xiaohong Liu, Xiangyang Sun, Xiaonan Mao, Zhaoye Wang, Jun Peng, Zuoren Yang, Faiza Ali, Zhi Wang, Fuguang Li

{"title":"The promotive and repressive effects of exogenous H2O2 on Arabidopsis seed germination and seedling establishment depend on application dose.","authors":"Yakong Wang, Xiaohong Liu, Xiangyang Sun, Xiaonan Mao, Zhaoye Wang, Jun Peng, Zuoren Yang, Faiza Ali, Zhi Wang, Fuguang Li","doi":"10.1111/ppl.70098","DOIUrl":"https://doi.org/10.1111/ppl.70098","url":null,"abstract":"Hydrogen peroxide (H2O2) displays significant and dual effects on seed germination and seedling development, depending on the application dosage. However, the definition of H2O2 thresholds and the mechanisms underlying the dual actions in Arabidopsis seed germination and seedling development are not yet clear. Here, we analyzed the Arabidopsis seed germination profiles in response to different concentrations of exogenous H2O2 and found that 2 mM functions as the key threshold, above this threshold, both seed germination and seedling establishment were gradually inhibited. By RNA-seq analysis and function verification, we identified pathways of abscisic acid (ABA) signalling, seed post-ripening, energy metabolism, ROS homeostasis, and cell wall loosening play positive roles in seed germination and seedling establishment downstream of the H2O2 signalling. Further physio-chemical approaches revealed that exogenous H2O2 affected the accumulation and distribution of O2 •- and H2O2 in embryonic tissues by regulating the tissue-specific expression of SDH2-3, RHD2, and PRXs. Collectively, we found that germination rate and aerial growth were positively correlated with endogenous H2O2 content and root length was positively correlated with O2 •- accumulation, demonstrating that different ROS signals played specific functions in different tissues and development processes. On the other hand, excessive H2O2 (10 mM) represses these two processes for radicle cell damage caused by oxidation stress. Finally, we put forward the mechanism model of the dual effects of exogenous H2O2 on seed germination and seedling establishment.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70098"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0