Physiologia plantarum最新文献_第10页

The C3H gene PtZFP2-like in Pinellia ternata acts as a positive regulator of the resistance to soft rot caused by Pectobacterium carotovorum. 半夏（Pinellia ternata） C3H基因PtZFP2-like对胡萝卜乳杆菌（Pectobacterium carotovorum）引起的软腐病抗性起正向调节作用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70121

Ming Luo, Xinyao Li, Jingyi Zhang, Yuhuan Miao, Dahui Liu

{"title":"The C3H gene PtZFP2-like in Pinellia ternata acts as a positive regulator of the resistance to soft rot caused by Pectobacterium carotovorum.","authors":"Ming Luo, Xinyao Li, Jingyi Zhang, Yuhuan Miao, Dahui Liu","doi":"10.1111/ppl.70121","DOIUrl":"10.1111/ppl.70121","url":null,"abstract":"Pinellia ternata (Thunb.) Breit is a member of the Araceae family and is globally distributed. The dry tuber has been used as a traditional Chinese medicine for over 2,000 years. With agricultural development, the harm of soft rot to P. ternata is an increasing problem. The lack of germplasm resources resistant to soft rot leads to less research on resistance mechanisms. In our study, we screened disease-resistant P. ternata P-1 and disease-susceptible P. ternata P-4 for the first time. Then, the infection of soft rot for 0, 24, and 48 hours was performed, and a de novo transcriptome analysis explored key genes associated with soft rot resistance. A total of 260,169 unigenes were identified and differentially expressed gene analysis was conducted. In total, 33 C3H-type ZFP genes were differentially expressed under Pectobacterium carotovorum infection. Transient expression of ZFP2-like (Cluster-5189.85444) resulted in a twofold increase at 24 hour post infection (hpi) and a threefold increase at 48 hpi in P-1 with soft rot infection, but no significant difference at P-4 enhanced the resistance of Nicotiana benthamiana to soft rot. Stable overexpression in P. ternata with a 2 ~ 11-fold increase in gene expression and reduced the lesion size from 6 mm to 2 ~ 4 mm at 24 hpi, demonstrating increased resistance to P. carotovorum. These findings indicated the ZFP2-like gene plays a pivotal role in soft rot resistance, enriches genetic data on disease resistance in P. ternata, and contributes to breed selection and improvement.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70121"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11837237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of hydrogen sulfide and nitric oxide in enhancing salt stress tolerance in cucumber seedlings. 硫化氢和一氧化氮在提高黄瓜幼苗耐盐性中的协同效应。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70109

Ritu Kumari, M Nasir Khan, Zubair Ahmad Parrey, Preedhi Kapoor, Bilal Ahmad Mir, Tuba Taziun, Parul Parihar, Gurmeen Rakhra

{"title":"Synergistic effects of hydrogen sulfide and nitric oxide in enhancing salt stress tolerance in cucumber seedlings.","authors":"Ritu Kumari, M Nasir Khan, Zubair Ahmad Parrey, Preedhi Kapoor, Bilal Ahmad Mir, Tuba Taziun, Parul Parihar, Gurmeen Rakhra","doi":"10.1111/ppl.70109","DOIUrl":"https://doi.org/10.1111/ppl.70109","url":null,"abstract":"Salinity stress poses a significant threat to plant growth and agricultural productivity, affecting millions of hectares of land worldwide. The adverse effects of salt toxicity, primarily caused by high levels of sodium chloride in soil and water, disrupt essential physiological processes in plants, leading to reduced yields and degraded soil quality. The present study thoroughly investigated the potential involvement of hydrogen sulphide (H2S) and nitric oxide (NO) in facilitating salt stress tolerance in cucumbers. In this investigation, NaHS (sodium hydrogen sulfide), which is the donor of H2S, and SNP (sodium nitroprusside), which is the donor of NO, were used as treatments for cucumber seedlings exposed to salt stress. Additionally, L-NAME (N-nitro-L-arginine: 100 μM) and cPTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide), which are inhibitors and scavengers of NO respectively, were used to verify the involvement of NO in the presence of salinity. NaHS and SNP supplementation significantly boosted fresh weight, dry weight, plant height, and chlorophyll content, promoting growth under salt stress. These treatments raised endogenous H2S and NO levels, upregulating antioxidative enzymes like SOD, CAT, APX, GR, GPX, and GSTs. This response reduced oxidative damages by lowering reactive oxygen species (ROS) and lipid peroxidation. The combined application of NaHS and SNP under salt stress offers a promising and cost-effective strategy to improve plant resilience to salinity, reduce oxidative stress, and ultimately enhance crop productivity. These findings provide important insights into the potential use of H2S and NO donors for sustaining agricultural production in saline environments, addressing a critical global challenge for food security.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70109"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigating Oxidative Browning in Litchi by Regulating Biochemical Markers and Targeted Gene expression via Exogenous Nitric Oxide. 通过外源一氧化氮调节生化标记物和靶向基因表达减轻荔枝氧化褐变。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70107

Khushboo Azam, Hidayatullah Mir, Mohammed Wasim Siddiqui, Tushar Ranjan, Fozia Homa, Nusrat Perveen, Duniya Ram Singh, Manzer H Siddiqui

{"title":"Mitigating Oxidative Browning in Litchi by Regulating Biochemical Markers and Targeted Gene expression via Exogenous Nitric Oxide.","authors":"Khushboo Azam, Hidayatullah Mir, Mohammed Wasim Siddiqui, Tushar Ranjan, Fozia Homa, Nusrat Perveen, Duniya Ram Singh, Manzer H Siddiqui","doi":"10.1111/ppl.70107","DOIUrl":"https://doi.org/10.1111/ppl.70107","url":null,"abstract":"Nitric oxide has been shown to influence oxidative metabolism in plants, enhancing their resilience to various biotic and abiotic stresses. Post-harvest oxidative stress is a key factor leading to quality deterioration in litchi (Litchi chinensis Sonn.) fruit, with visible symptoms that significantly reduce shelf life and consumer acceptability. Therefore, the effect of exogenous sodium nitroprusside (SNP; 1.0 mM and 2.0 mM) on litchi (cv. Purbi) fruit was examined during storage at 7 ± 1°C. Different biochemical changes related to post-harvest quality and pericarp browning of litchi were evaluated. The results suggested that SNP (2.0 mM) was significantly effective in reducing weight loss, the pericarp browning index and decay loss. The fruit subjected to SNP (2.0 mM) treatment retained more total anthocyanins and total phenolic content with reduced peroxidase and polyphenol oxidase enzyme activity. Other quality attributes, such as total soluble solids (TSS), titratable acidity and ascorbic acid, were also recorded to be greater in the SNP (2.0 mM)-treated fruits. These results were consistent with the expression profiles of LcPPO, LcPOD and Laccase genes. The expression levels of these genes were highly suppressed in the nitric oxide-treated fruits compared to those in the control fruits. Therefore, SNP (2.0 mM) treatment could reduce litchi pericarp browning and prolong the post-harvest life of fruit for up to eighteen days during cold storage.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70107"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of microplastics and salt single or combined stresses on growth and physiological responses of maize seedlings. 微塑料和盐单一或复合胁迫对玉米幼苗生长和生理反应的影响

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70106

Xiaodong Liu, Zongshuai Wang, Guiyang Shi, Yingbo Gao, Hui Zhang, Kaichang Liu

{"title":"Effects of microplastics and salt single or combined stresses on growth and physiological responses of maize seedlings.","authors":"Xiaodong Liu, Zongshuai Wang, Guiyang Shi, Yingbo Gao, Hui Zhang, Kaichang Liu","doi":"10.1111/ppl.70106","DOIUrl":"https://doi.org/10.1111/ppl.70106","url":null,"abstract":"Plastic film (mulch film) is widely used in saline and alkaline soils because it can effectively reduce salt stress damage. However, it results in the accumulation of microplastics (MPs) in the soil, which pose a threat to crop growth and production. This study investigates the effects of 50 mg l-1 MPs and 100 mM sodium chloride (NaCl), individually or in combination, on the growth and physiological characteristics of maize (Zea mays) seedlings. The results demonstrated that compared to the control, MPs and NaCl single or combined stress reduced seedling biomass and water content, and the combined stress was more serious. Stress significantly reduced N and K contents in leaves, and Na content under combined stress was lower than under single NaCl stress. Compared to single stress, the combined stress further enhanced oxidative damage by increasing H2O2 and MDA content, a disrupted chloroplast structure, and reduced chlorophyll content, ultimately leading to a decline in chlorophyll fluorescence parameters and photosynthetic efficiency. Single MPs or NaCl stress led to the accumulation of proline, soluble proteins, and soluble sugars, while the combined stresses further increased the content of these osmotic substances in plants. Moreover, single or combined stress increased the activity of CAT, POD, SOD and the content of AsA and GsH. Collectively, NaCl and MPs single or combined stress exert notable toxic effects on maize seedling growth. Although the combined stress inhibited seedling growth more than the single stress, the combined stress of MPs and NaCl showed antagonistic effects. These findings underscore the importance of assessing the ecological risks posed by the combined effects of MPs and salt stresses on maize plants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70106"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic in vivo monitoring of granum structural changes of Ctenanthe setosa (Roscoe) Eichler during drought stress and subsequent recovery.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.14621

Richard Hembrom, Renáta Ünnep, Éva Sárvári, Gergely Nagy, Katalin Solymosi

{"title":"Dynamic in vivo monitoring of granum structural changes of Ctenanthe setosa (Roscoe) Eichler during drought stress and subsequent recovery.","authors":"Richard Hembrom, Renáta Ünnep, Éva Sárvári, Gergely Nagy, Katalin Solymosi","doi":"10.1111/ppl.14621","DOIUrl":"10.1111/ppl.14621","url":null,"abstract":"Investigating the effects of drought stress and subsequent recovery on the structure and function of chloroplasts is essential to understanding how plants adapt to environmental stressors. We investigated Ctenanthe setosa (Roscoe) Eichler, an ornamental plant that can tolerate prolonged drought periods (40 and 49 days of water withdrawal). Conventional biochemical, biophysical, physiological and (ultra)structural methods combined for the first time in a higher plant with in vivo small-angle neutron scattering (SANS) were used to characterize the alterations induced by drought stress and subsequent recovery. Upon drought stress, no significant changes occurred in the chloroplast ultrastructure, chlorophyll content, 77K fluorescence emission spectra and maximal quantum efficiency of PSII (Qy dark), but the actual quantum efficiency of PSII (Qy light) decreased, the amounts of PSI-LHCII complexes and PSII monomers declined, and that of PSII supercomplexes increased. Thickness of the leaf and of the adaxial hypodermis, chloroplast length and granum repeat distance (RD) values decreased upon drought stress, as shown by light microscopy and SANS, respectively. Because of the very slight (nm-range) changes in RD values, the large biological variability (significant differences in RD values among the leaves and studied leaf regions) and the invasive sampling required for this method, transmission electron microscopy (TEM) hardly showed significant differences. On the other side, in situ SANS analyses provided a unique insight in vivo into the fast structural recovery of the granum structure of drought-stressed leaves, which happened already 18 h after re-watering, while functional and biochemical recovery took place on a longer time scale.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e14621"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of GmLFYa and GmLFYb on Compound Leaf Development in Soybean.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70092

Dongfa Wang, Baolin Zhao, Xuan Zhou, Shaoli Zhou, Liling Yang, Yawen Mao, Quanzi Bai, Weiyue Zhao, Mingzhu Sun, Mingli Liu, Zhijia Gu, Liangliang He, Jianghua Chen

{"title":"Synergistic effects of GmLFYa and GmLFYb on Compound Leaf Development in Soybean.","authors":"Dongfa Wang, Baolin Zhao, Xuan Zhou, Shaoli Zhou, Liling Yang, Yawen Mao, Quanzi Bai, Weiyue Zhao, Mingzhu Sun, Mingli Liu, Zhijia Gu, Liangliang He, Jianghua Chen","doi":"10.1111/ppl.70092","DOIUrl":"https://doi.org/10.1111/ppl.70092","url":null,"abstract":"Legume leaves exhibit diverse compound forms, with various regulatory mechanisms underlying the development. The transcription factor-encoding KNOXI genes are required to promote leaflet initiation in most compound-leafed angiosperms. In non-IRLC (inverted repeat-lacking clade) legumes, KNOXI are expressed in compound leaf primordia but not in others (IRLC). Recent studies have highlighted LFY genes' role in regulating leaflet initiation across legumes. The LFY functions in leaf development are well understood in IRLC legumes but remain unclear in non-IRLC legumes. Soybean, a major crop belonging to non-IRLC legumes, has limited research on the trifoliate leaf morphogenesis. Here, we comprehensively analyzed soybean trifoliate leaf development and characterized two GmLFY gene copies, GmLFYa and GmLFYb, in compound leaf morphogenesis. Analyzing the loss-of-function mutants revealed that Gmlfya displayed a low frequency of simple-like leaves, while the Gmlfyb showed no visible phenotype. However, the Gmlfya Gmlfyb double mutant predominantly displayed simple-like leaves. Additionally, mutations in two genes also affect floral development: each single mutant exhibited slightly deformed floral organs, while double mutant produced inflorescence-like structures. The transformation from floral meristems to inflorescence-like structures is similar to lfy mutant in Arabidopsis but quite different from M. truncatula and L. japonicus. These findings suggest that the two GmLFY genes in soybean collaboratively regulate both compound leaf and flower morphogenesis. Our study not only creates foundational mutant materials for future research on leaf and flower development in soybean but also reinforces the role of LFY orthologs as master regulators in compound leaf morphogenesis across a broader range of legume taxa than previously recognized.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70092"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GhRac9 improves cotton resistance to Verticillium dahliae via regulating ROS production and lignin content.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70091

Xincheng Luo, Zongwei Hu, Longyan Chu, Jianping Li, Ziru Tang, Xiangxiang Sun, Hongliu An, Peng Wan, Xiangping Wang, Yazhen Yang, Jianmin Zhang

{"title":"GhRac9 improves cotton resistance to Verticillium dahliae via regulating ROS production and lignin content.","authors":"Xincheng Luo, Zongwei Hu, Longyan Chu, Jianping Li, Ziru Tang, Xiangxiang Sun, Hongliu An, Peng Wan, Xiangping Wang, Yazhen Yang, Jianmin Zhang","doi":"10.1111/ppl.70091","DOIUrl":"https://doi.org/10.1111/ppl.70091","url":null,"abstract":"Rac/Rop proteins, a kind of unique small GTPases in plants, play crucial roles in plant growth and development and in response to abiotic and biotic stresses. However, it is poorly understood whether cotton Rac/Rop protein genes are involved in mediating cotton resistance to Verticillium dahliae. Here, we focused on the function and mechanism of cotton Rac/Rop gene GhRac9 in the defense response to Verticillium dahliae infection. The expression level of GhRac9 peaked at 24 h after V. dahliae infection and remained consistently elevated from 24 to 48 h upon SA treatment. Furthermore, silencing GhRac9 using VIGS (Virus-induced gene silence) method attenuated cotton defense response to V. dahliae by reducing ROS (Reactive Oxygen Species) burst, peroxidase activity and lignin content in cotton plants. On the contrary, heterologous overexpression of GhRac9 enhanced Arabidopsis resistance to V. dahliae and significantly increased ROS production in Arabidopsis plants. Furthemore, transient overexpressing of GhRac9 significantly enhanced ROS burst and POD activity in cotton plants. In addition, GhRac9 positively regulated the expression levels of the genes related to SA signaling pathway in cotton plants. In conclusion, GhRac9 functioned as a positive regulator in the cotton defense response to V. dahliae, which provided important insights for breeding new cotton varieties resistant to V. dahliae.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70091"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boron controls apical dominance in Pea (Pisum sativum) via promoting polar auxin transport. 硼通过促进植物生长素的极性运输来控制豌豆的顶端优势。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70056

Yutong He, Keren He, Jingwen Mai, Meiyin Ou, Laibin Chen, Yuanyuan Li, Tao Wan, Luping Gu, Sergey Shabala, Xuewen Li, Yalin Li, Min Yu

{"title":"Boron controls apical dominance in Pea (Pisum sativum) via promoting polar auxin transport.","authors":"Yutong He, Keren He, Jingwen Mai, Meiyin Ou, Laibin Chen, Yuanyuan Li, Tao Wan, Luping Gu, Sergey Shabala, Xuewen Li, Yalin Li, Min Yu","doi":"10.1111/ppl.70056","DOIUrl":"https://doi.org/10.1111/ppl.70056","url":null,"abstract":"Plant architecture and subsequent productivity are determined by the shoot apical dominance, which is disturbed by the deficiency of boron, one of the essential trace elements for plant growth and reproduction. However, the mechanism by which B controls shoot apical dominance or axillary bud outgrows under B deficiency is still unclear. This work aimed to investigate the mechanistic basis of this process, with focus on the interaction between B and polar auxin transport. Adopting an all-buds phenotyping methodology and employing several complementary approaches, we found that boron deficiency inhibited plant growth and changed the shoot architecture, resulting in the outgrowth of axillary buds at nodes 1-3. This was related to the auxin accumulation in shoot apical parts buds under B deficiency. Applying N-1-naphthylphthalamic acid to inhibit auxin transport from the shoot apex promoted the outgrowth of axillary buds in boron-sufficient (+B) plants. In decapitated plants, the application of exogenous auxin to the shoot apex only inhibited the outgrowth of axillary buds in +B plants. At higher auxin doses, the toxic effect of IAA was observed in the lower part of the shoot, which was more severe in +B plants than in B-deprived (-B) plants. Furthermore, the expression of PsPIN3 was significantly downregulated under -B conditions. These results indicate that B deficiency inhibits PAT from the apical bud through the main stem to the lower parts, leading to an increase of auxin level in the apical bud, which inhibits the growth of apical buds while stimulating the outgrowth of axillary buds.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70056"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep metabolomics revealed trajectories of jasmonate signaling-mediated primary metabolism in Arabidopsis upon Spodoptera litura herbivory. 深层代谢组学揭示了拟南芥在斜纹夜蛾（Spodoptera litura）草食过程中茉莉酸信号介导的初级代谢轨迹。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70035

Anish Kundu, Paramita Bera, Shruti Mishra, Jyothilakshmi Vadassery

{"title":"Deep metabolomics revealed trajectories of jasmonate signaling-mediated primary metabolism in Arabidopsis upon Spodoptera litura herbivory.","authors":"Anish Kundu, Paramita Bera, Shruti Mishra, Jyothilakshmi Vadassery","doi":"10.1111/ppl.70035","DOIUrl":"https://doi.org/10.1111/ppl.70035","url":null,"abstract":"Plants defend against chewing herbivores by up-regulating jasmonic acid (JA) signaling, which activates downstream signaling cascades and produces numerous secondary metabolites that act as defense molecules against the herbivores. Although secondary metabolism always remains a focus of research, primary metabolism is also reported to be realigned upon herbivory. However, JA signaling-mediated modulation of primary metabolites and their metabolic pathways in plants are mostly unexplored. Here, we applied gas chromatography-mass spectrometry-based untargeted metabolomics aided with computational statistical frameworks on wild type Arabidopsis, mutants of active JA receptor (i.e., CORONATINE-INSENSITIVE 1, COI1-1) and downstream transcription factor (i.e., MYC2) to navigate the JA signaling-mediated primary metabolism alterations during herbivory. Pathway and metabolite's chemical class enrichment analysis revealed JA signaling is crucial for constitutive as well as herbivore-induced primary metabolism and topology of their interaction networks. JA signaling majorly modulated alterations of sugars, amino acids and related metabolites. Herbivory-mediated sugar depletion and induction of methionine for aliphatic glucosinolates are also dependent on JA signaling. Taken together, our results demonstrate trails of JA signaling-mediated primary metabolic alterations associated with herbivory.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70035"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upgrading of the genetic engineering toolkit accelerated the discovery process of the virulence effect of PsGH7d on Phytophthora sojae invasion. 基因工程工具箱的升级加速了PsGH7d对大豆疫霉入侵毒力效应的发现过程。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70083

Changqing Liu, Xinwei Tan, Jiayu Wang, Yujing Sun, Qian Xu, Chao Han, Qunqing Wang

{"title":"Upgrading of the genetic engineering toolkit accelerated the discovery process of the virulence effect of PsGH7d on Phytophthora sojae invasion.","authors":"Changqing Liu, Xinwei Tan, Jiayu Wang, Yujing Sun, Qian Xu, Chao Han, Qunqing Wang","doi":"10.1111/ppl.70083","DOIUrl":"https://doi.org/10.1111/ppl.70083","url":null,"abstract":"The genus of Phytophthora includes numerous phytopathogens that have devastating impacts on agricultural production. However, the limited availability of selection markers for numerous pathogenicity pathogens of the genus Phytophthora genetic transformation hinders further research on their pathogenic functional genes. Here we report a gene of NAT I, which serves as a novel selection marker for the Phytophthora sojae transformation. Additionally, we developed a new genetic manipulation toolkit based on vectors containing NAT I, which facilitates gene editing in P. sojae. With the toolkit, the gene PsGH7d of P. sojae, which encodes a glycosyl hydrolase, was edited consecutively via the CRISPR/Cas9 system to obtain gene knockout and enzymatic active site mutation strains. The pathogenicity analysis of these transformants revealed that PsGH7d is a virulence factor dependent on its bifunctional glucanase-xylanase activities. This study develops an updated toolkit for the genus Phytophthora genetic transformation and provides initial insights into the virulence of the bifunctional enzyme PsGH7d.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70083"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0