Physiologia plantarum最新文献_第10页

Identification, Evolution, and Expression Analysis of Ten Gene Families of General Phenylpropanoid and Monolignol-Specific Metabolism in Cucumber. 黄瓜一般苯丙素和单木质素特异性代谢10个基因家族的鉴定、进化及表达分析

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-05-01 DOI: 10.1111/ppl.70300

Menglin Zhang, Ziyue Zeng, Caiyun Sun, Shenglin Wang, Rui Hu, Yujian Liu, Chao Yu, Shengjun Zhou, Jingtao Nie

{"title":"Identification, Evolution, and Expression Analysis of Ten Gene Families of General Phenylpropanoid and Monolignol-Specific Metabolism in Cucumber.","authors":"Menglin Zhang, Ziyue Zeng, Caiyun Sun, Shenglin Wang, Rui Hu, Yujian Liu, Chao Yu, Shengjun Zhou, Jingtao Nie","doi":"10.1111/ppl.70300","DOIUrl":"https://doi.org/10.1111/ppl.70300","url":null,"abstract":"As the main component of secondary thickened plant cell walls, lignin plays a crucial role in plant development and protection from biotic and abiotic stressors. The lignin biosynthesis pathway has not been extensively studied in cucumber, an important vegetable cultivated worldwide. Therefore, this study identified and characterised the family members of general phenylpropanoid and monolignol-specific metabolism in cucumber. We identified 10 gene families comprising 84 enzyme genes distributed across 7 chromosomes. Phylogenetic, collinearity, and motif analyses revealed significant conservation of family members among different species. Furthermore, expression analysis was performed using transcriptome data and qRT-PCR. These genes exhibited organ-specific expression patterns, and their expression occurred in response to biotic and abiotic stress. Subcellular localisation experiments indicated that CsHCT12 and CsCAD2 were localised in the cytoplasm and the plasma membrane. Furthermore, potential miRNA-targeted analysis identified 84 family genes in cucumber that could be regulated by 403 miRNAs. The transcription factor (TF) regulatory network revealed 118 TFs that may regulate 78 family genes. Luciferase assays have indicated that CsWRKY50 can activate the expression of CsPAL12 and Cs4CL10. These findings lay the foundation for further exploration of the functional roles of gene family members of the general phenylpropanoid and monolignol-specific metabolism in cucumber.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70300"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved photorespiration has a major impact on the root metabolome of Arabidopsis. 光呼吸改善对拟南芥根系代谢组有重要影响。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70142

Stefan Timm, Alexandra Florian, Saleh Alseekh, Kathrin Jahnke, Martin Hagemann, Alisdair R Fernie, Hermann Bauwe

{"title":"Improved photorespiration has a major impact on the root metabolome of Arabidopsis.","authors":"Stefan Timm, Alexandra Florian, Saleh Alseekh, Kathrin Jahnke, Martin Hagemann, Alisdair R Fernie, Hermann Bauwe","doi":"10.1111/ppl.70142","DOIUrl":"10.1111/ppl.70142","url":null,"abstract":"Photorespiration is an essential metabolic repair process in oxygenic photosynthesis, as it detoxifies Rubisco's inhibitory oxygenase byproduct, 2-phosphoglycolate (2-PG). It has been demonstrated that improving endogenous photorespiration in C3 plants through enzyme overexpression can enhance photosynthesis and promote plant growth. However, the potential impact of improved photorespiration in leaves on heterotrophic roots remained unexplored. To address this, we conducted a metabolome analysis of Arabidopsis leaves and roots using transgenic lines with enhanced glycine decarboxylase (GDC) activity, achieved by overexpressing the mitochondrial lipoamide dehydrogenase (mtLPD1) subunit. In the leaves, mtLPD1 overexpression primarily resulted in reduced steady-state levels of intermediates associated with photorespiration, the tricarboxylic acid (TCA) cycle, and soluble sugars, while intermediates related to nitrogen metabolism were elevated. In roots, where mtLPD1 expression was unchanged, we observed contrasting accumulation patterns in the transgenic lines compared to the wildtype, including increased levels of photorespiratory and TCA-cycle intermediates. Notably, we also detected elevated amounts of soluble sugars, nitrate, and starch. Phloem exudate analysis revealed altered metabolite profiles in the overexpressors, particularly with respect to photorespiratory intermediates linked to the GDC reaction, as well as soluble sugars and metabolites involved in cellular redox homeostasis. This suggested an increased transport of these metabolites from shoots to roots, thereby altering sink organ metabolism. In summary, we hypothesize that optimizing photorespiration enhances photosynthesis, which leads to an increased export of carbon surplus to heterotrophic tissues. Thus, improving photorespiration may hold potential for increasing yields in beet- and tuber-forming plants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70142"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The HmERF1-HmbZIP1 module increases powdery mildew resistance by inhibiting HmSWEET1 sugars transporting in Heracleum moellendorffii Hance. HmERF1-HmbZIP1模块通过抑制Heracleum moellendorffii Hance中HmSWEET1糖的运输来提高白粉病抗性。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70145

Liu Hanbing, Liu Junxia, Zhang Yong, Cao Ning, Jiang Xinmei, Tong Xuejiao, Yu Xihong, Cheng Yao

{"title":"The HmERF1-HmbZIP1 module increases powdery mildew resistance by inhibiting HmSWEET1 sugars transporting in Heracleum moellendorffii Hance.","authors":"Liu Hanbing, Liu Junxia, Zhang Yong, Cao Ning, Jiang Xinmei, Tong Xuejiao, Yu Xihong, Cheng Yao","doi":"10.1111/ppl.70145","DOIUrl":"10.1111/ppl.70145","url":null,"abstract":"Powdery mildew (PM) caused by Eeysiphe heraclei is a serious concern in Heracleum moellendorffii Hance. E. heraclei is a biotrophic fungus that absorbs glucose as the major carbon energy source, using haustoria after infection. However, the mechanisms of sugar efflux from host cells to the fungus remain undetermined. Our previous study revealed that E. heraclei infection altered sugar transfer and distribution in H. moellendorffii, and that increased sugar concentrated in the infected regions. Here, RNA-sequencing was used to identify a key sugar transporter, HmSWEET1, which transported hexose sugars. Overexpression or silencing of the HmSWEET1 gene in H. moellendorffii enhanced or reduced resistance to PM by regulating sugar concentrations in infection sites. Further analysis identified two key transcription factors, HmERF1 and HmbZIP1, which are bound to the HmSWEET1 promoter, inhibit the gene expression. Furthermore, overexpression of HmERF1 and HmbZIP1 in H. moellendorffii enhanced plant resistance to PM by interfering with the ability of HmSWEET1 to transport sugars, thereby decreasing the sugar concentrations in infected leaf areas. Moreover, HmERF1 interaction with HmbZIP1 in H. moellendorffii further enhanced plant resistance. The results identified a novel HmERF1-HmbZIP1-HmSWEET1 module, which strengthened PM' resistance by reducing sugar supplies in H. moellendorffii through suppression of sugar transport by HmSWEET1.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70145"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic regulation and beyond in grapevine-pathogen interactions: a biotechnological perspective. 表观遗传调控和超越葡萄藤-病原体相互作用：生物技术的观点。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70216

João Proença Pereira, Ivan Bevilacqua, Rita B Santos, Serena Varotto, Walter Chitarra, Luca Nerva, Andreia Figueiredo

{"title":"Epigenetic regulation and beyond in grapevine-pathogen interactions: a biotechnological perspective.","authors":"João Proença Pereira, Ivan Bevilacqua, Rita B Santos, Serena Varotto, Walter Chitarra, Luca Nerva, Andreia Figueiredo","doi":"10.1111/ppl.70216","DOIUrl":"https://doi.org/10.1111/ppl.70216","url":null,"abstract":"As one of the most important crop plants worldwide, understanding the mechanisms underlying grapevine response to pathogen attacks is key to achieving a productive and sustainable viticulture. Recently, epigenetic regulation in plant immunity has gained significant traction in the scientific community, not only for its role in gene expression regulation but also for its heritability, giving it enormous biotechnological potential. Epigenetic marks have been shown to be dynamically modulated in key genomic regions upon infection, with some being maintained after such, being responsible for priming defense genes. In grapevine, however, knowledge of epigenetic mechanisms is still limited, especially regarding biotic stress responses, representing a glaring gap in knowledge in this important crop plant. Here, we report and integrate current knowledge on grapevine epigenetic regulation as well as non-epigenetic non-coding RNAs in the response to biotic stress. We also explore how epigenetic marks may be useful in grapevine breeding for resistance, considering different approaches, from uncovering and exploiting natural variation to inducing it through different means.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70216"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11999821/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic communications in characean internodes at neutral and alkaline external pH. 中性和碱性外部pH下特征节间的微流控通讯。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70211

Alexander A Bulychev, Natalia A Krupenina

{"title":"Microfluidic communications in characean internodes at neutral and alkaline external pH.","authors":"Alexander A Bulychev, Natalia A Krupenina","doi":"10.1111/ppl.70211","DOIUrl":"https://doi.org/10.1111/ppl.70211","url":null,"abstract":"Intracellular communications mediated by cytoplasmic streaming compensate for the slowness of diffusion on large scale distances. In characean internodes, cyclosis serves to smooth concentration gradients related to local structural distinctions, irregular spotted illumination, and patterned profiles of external pH. In dimly lit Chara cells, the fluidic transmission of reducing equivalents from the spot of bright light incidence to a remote analyzed area transiently elevates the actual yield of chlorophyll fluorescence (F') under natural acidic zones with little effect on F' under alkaline bands. Here, the natural formation of alkaline zones was imitated by placing the internodal cell part into a solution with a pH of 9.5. Using PAM microfluorometry, we found that chloroplasts located under an alkaline solution retained the perception of reducing equivalents transported with the fluid flow but, in addition, became responsive to another transportable metabolite that promoted strong quenching of both F'm and F' fluorescence. The superposition of oppositely directed F' responses to distinct cyclosis-transported metabolites resulted in the seeming suppression of microfluidic interactions between distant chloroplasts. The action potential generation did not affect F'm fluorescence (an indicator of non-photochemical quenching, NPQ) when the cell was bathed at neutral pH but induced strong NPQ in the high pH solution. We propose that the restricted CO2 supply at high external pH induces the rearrangement of electron transport to alternative pathways, which elevates the background level of NPQ-promoting metabolite (supposedly H2O2), thus enhancing the chloroplast sensitivity to H2O2 portions delivered with the fluid flow from the region subjected to intense local light.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70211"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unresolved roles of Aux/IAA proteins in auxin responses. Aux/IAA蛋白在生长素反应中的作用尚未确定。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70221

Monika Kubalová, Martina Schmidtová, Matyáš Fendrych

{"title":"Unresolved roles of Aux/IAA proteins in auxin responses.","authors":"Monika Kubalová, Martina Schmidtová, Matyáš Fendrych","doi":"10.1111/ppl.70221","DOIUrl":"https://doi.org/10.1111/ppl.70221","url":null,"abstract":"Aux/IAA proteins are well-known as key components of the nuclear auxin signaling pathway, repressing gene transcription when present and enabling gene activation upon their degradation. In this review, we explore the additional roles of Aux/IAA proteins in the known auxin perception pathways-the TIR1/AFBs nuclear as well as in the emerging cytoplasmic and apoplastic pathways. We summarize recent advances in understanding the regulation of Aux/IAA protein stability at the post-translational level, a critical factor in auxin-regulated transcriptional output. We further highlight the roles of auxin-nondegradable non-canonical Aux/IAAs in auxin-mediated transcription and their involvement in apoplastic auxin signalling. Additionally, we discuss the importance of Aux/IAAs for the adenylate cyclase activity of TIR1/AFB receptors and speculate on their involvement in the cytoplasmic auxin pathway. Using Arabidopsis root as a model, this work underscores the central role of Aux/IAA proteins in mediating auxin-driven developmental processes and environmental responses. Key questions for future research are proposed to further unravel the dynamic roles of Aux/IAAs in auxin signaling networks.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70221"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mathematical analysis of long-distance polar auxin transport data of pin mutants questions the role of PIN1 as postulated in the chemi-osmotic theory. 对pin突变体长距离极性生长素运输数据的数学分析质疑了PIN1在化学渗透理论中的作用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70139

Kees J M Boot, Sander C Hille, Kees R Libbenga, Marijke Libbenga-Nijkamp, Omid Karami, Bert Van Duijn, Remko Offringa

{"title":"Mathematical analysis of long-distance polar auxin transport data of pin mutants questions the role of PIN1 as postulated in the chemi-osmotic theory.","authors":"Kees J M Boot, Sander C Hille, Kees R Libbenga, Marijke Libbenga-Nijkamp, Omid Karami, Bert Van Duijn, Remko Offringa","doi":"10.1111/ppl.70139","DOIUrl":"10.1111/ppl.70139","url":null,"abstract":"The plant hormone auxin (Indole-3-Acetic Acid, IAA) is a key player in nearly every aspect of plant growth and development ranging from cell division and cell elongation to embryogenesis and root formation. The IAA level in specific tissues and cells is regulated by synthesis, conjugation, degradation and transport. Especially long-range polar auxin transport (PAT) has been the subject of numerous studies. The chemi-osmotic theory predicts that intercellular PAT is caused by an asymmetric distribution of auxin efflux transporters in cell membranes of transporting cells, resulting in increased local membrane permeability for IAA. Members of the PIN gene family are generally considered to encode the postulated carriers. The objective of this study was to use the chemi-osmotic theory in an experimental program aimed at describing and interpreting long-range PAT data from mutants of the PIN gene family of Arabidopsis thaliana. Therefore, we put the chemi-osmotic theory in a broader theoretical framework. We find that the observed decrease in both auxin flux and transport velocity in pin1 loss-of-function mutants is not caused by decreased basal membrane permeability, as would be expected according to the chemi-osmotic theory, but is an indirect effect caused by a change in the dynamics of auxin transport due to a decrease in the expression of all four AUX1/LAX1-3 auxin influx carriers in pin1 mutants. On the basis of our findings, we conclude that the exact role of PIN1 in long-distance PAT, as postulated in the chemi-osmotic theory, should be reconsidered.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70139"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing plant-derived DNA repair enzyme resources through studying the involvement of base excision repair DNA glycosylases in stress responses of plants. 通过研究碱基切除修复 DNA 糖基化酶在植物应激反应中的参与情况，开发植物 DNA 修复酶资源。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70162

Ying Zhao, Daniel Chen, Inga R Grin, Dmitry O Zharkov, Bing Yu

引用次数: 0

Interaction between pathogenesis-related (PR) proteins and phytohormone signaling pathways in conferring disease tolerance in plants. 发病相关蛋白（PR）与植物激素信号通路在植物抗病性中的相互作用

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70174

Paramdeep Kumar, Saurabh Pandey, Pratap Kumar Pati

{"title":"Interaction between pathogenesis-related (PR) proteins and phytohormone signaling pathways in conferring disease tolerance in plants.","authors":"Paramdeep Kumar, Saurabh Pandey, Pratap Kumar Pati","doi":"10.1111/ppl.70174","DOIUrl":"10.1111/ppl.70174","url":null,"abstract":"Pathogenesis-related (PR) proteins are critical defense signaling molecules induced by phytopathogens. They play a vital role in plant's defense signaling pathways and innate immunity, particularly in systemic acquired resistance (SAR) and serve as key molecular markers of plant defense. Overexpressing PR genes, such as chitinase, thaumatin, glucanase, thionin and defensin, either individually or in combination, have significantly boosted plants' defense responses against various pathogens. However, signaling pathways regulating the expression of these versatile proteins remain only partially understood. Plant hormones like salicylic acid (SA) and jasmonic acid (JA) are known for their well-established roles in regulating PR gene responses to pathogens and other stress conditions. PR genes interact with various components of hormonal signaling pathways, including receptors (e.g., NPR1 in SA signaling), transcription factors (e.g., MYC2 in JA signaling), and cis-regulating elements (e.g., W-box), to modulate plant defense responses. Recent studies have highlighted the contributions of different plant hormones to plant immunity and their interactions with PR proteins in a process known as hormonal crosstalk, which helps coordinate immunity activation. This review provides a comprehensive overview of the PR proteins, their complexity, and hormonal crosstalk in immunity, aiming to understand these interactions for improved pathogen resistance.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70174"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

WRKY75-mediated transcriptional regulation of OASA1 controls leaf senescence in Arabidopsis. wrky75介导的OASA1转录调控调控拟南芥叶片衰老。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70193

Qijun Ma, Shuo Xu, Shi Hu, Kaijing Zuo

引用次数: 0