Physiologia plantarum最新文献_第9页

Mutation of the K⁺ transporter SlHAK5 of tomato alters pistil morphology, ionome, metabolome and transcriptome in flowers. 番茄 K+ 转运体 SlHAK5 的突变会改变花的雌蕊形态、离子组、代谢组和转录组。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14585

Jesús Amo, Elisa Jiménez-Estévez, Almudena Martínez-Martínez, Adrián Yáñez, Vicente Martínez, Manuel Nieves-Cordones, Francisco Rubio

{"title":"Mutation of the K+ transporter SlHAK5 of tomato alters pistil morphology, ionome, metabolome and transcriptome in flowers.","authors":"Jesús Amo, Elisa Jiménez-Estévez, Almudena Martínez-Martínez, Adrián Yáñez, Vicente Martínez, Manuel Nieves-Cordones, Francisco Rubio","doi":"10.1111/ppl.14585","DOIUrl":"https://doi.org/10.1111/ppl.14585","url":null,"abstract":"K+ accumulation in plant tissues is a crucial factor for plant growth and development. The tomato high-affinity K+ transporter SlHAK5 is essential for root K+ acquisition from low external concentrations. It is also involved in K+ accumulation in pollen and plant fertility as slhak5 KO plants show a low rate of pollen germination, impaired pollen tube growth and parthenocarpic fruits. Here, we present a thorough analysis of slhak5 flowers, which showed relevant defects at the anatomic, ionomic, metabolomic and transcriptomic levels. First, slhak5 flowers exhibited shorter styles and enlarged ovaries that, together with a low number of seeds in fruits from slhak5 X WT crosses, indicated an effect of the slhak5 mutation on female fertility. Second, a lower accumulation of Ca2+, as well as of several metabolites such as amino acids, citric acid and sugars, was observed in mutant flowers, whereas indole-3-acetic acid content was increased when compared to the wild-type. Third, RNAseq conducted on pistils and stamens of wild-type and slhak5 plants revealed that transport and signalling pathways are significantly enriched in the gene expression analyses of stamens. Thus, it can be concluded that a functional SlHAK5 transporter is required to maintain appropriate Ca2+, metabolite and gene expression levels in flowers, and its absence leads to important reductions in both male and female fertility.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14585"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

R2R3-MYB repressor, BrMYB32, regulates anthocyanin biosynthesis in Chinese cabbage. R2R3-MYB抑制因子BrMYB32调控大白菜花青素的生物合成。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14591

Sun-Hyung Lim, Da-Hye Kim, Jong-Yeol Lee

{"title":"R2R3-MYB repressor, BrMYB32, regulates anthocyanin biosynthesis in Chinese cabbage.","authors":"Sun-Hyung Lim, Da-Hye Kim, Jong-Yeol Lee","doi":"10.1111/ppl.14591","DOIUrl":"10.1111/ppl.14591","url":null,"abstract":"Anthocyanin-enriched Chinese cabbage has health-enhancing antioxidant properties. Although various regulators of anthocyanin biosynthesis have been identified, the role of individual repressors in this process remains underexplored. This study identifies and characterizes the R2R3-MYB BrMYB32 in Chinese cabbage (Brassica rapa), which acts as a repressor in anthocyanin biosynthesis. BrMYB32 expression is significantly upregulated under anthocyanin inductive conditions, such as sucrose and high light treatment. Transgenic tobacco plants overexpressing BrMYB32 show decreased anthocyanin levels and downregulation of anthocyanin biosynthesis genes in flowers, highlighting BrMYB32's repressive role. Located in the nucleus, BrMYB32 interacts with the TRANSPARENT TESTA 8 (BrTT8), a basic helix-loop-helix protein, but no interaction was detected with the R2R3-MYB protein PRODUCTION OF ANTHOCYANIN PIGMENT 1 (BrPAP1). Functional assays in Chinese cabbage cotyledons and tobacco leaves demonstrate that BrMYB32 represses the transcript level of anthocyanin biosynthesis genes, thereby inhibiting pigment accumulation. Promoter activation assays further reveal that BrMYB32 inhibits the transactivation of CHALCONE SYNTHASE and DIHYDROFLAVONOL REDUCTASE through the C1 and C2 motifs. Notably, BrMYB32 expression is induced by BrPAP1, either alone or in co-expression with BrTT8, and subsequently regulates the expression of these activators. It verifies that BrMYB32 not only interferes with the formation of an active MYB-bHLH-WD40 complex but also downregulates the transcript levels of anthocyanin biosynthesis genes, thereby fine-tuning anthocyanin biosynthesis. Our findings suggest a model in which anthocyanin biosynthesis in Chinese cabbage is precisely regulated by the interplay between activators and repressors.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14591"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methyl jasmonate mitigates Fusarium graminearum infection in wheat by inhibiting deoxynivalenol synthesis. 茉莉酸甲酯通过抑制脱氧雪腐镰刀菌烯醇的合成，减轻小麦中禾本科镰刀菌的感染。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14593

Jing Gao, Yidan Sun, Weiguo Jin, Feng Zhang, Mingguo Zhou, Xiushi Song

{"title":"Methyl jasmonate mitigates Fusarium graminearum infection in wheat by inhibiting deoxynivalenol synthesis.","authors":"Jing Gao, Yidan Sun, Weiguo Jin, Feng Zhang, Mingguo Zhou, Xiushi Song","doi":"10.1111/ppl.14593","DOIUrl":"https://doi.org/10.1111/ppl.14593","url":null,"abstract":"Methyl jasmonate (MeJA), a plant growth regulator, coordinates a diverse array of physiological responses, including the inhibition of seed germination, modulation of secondary metabolite biosynthesis, and activation of defence responses. The external application of MeJA has been demonstrated to effectively diminish the severity of fungal diseases. Here, we unveil a novel mechanism through which exogenous MeJA alleviates Fusarium head blight (FHB) by inhibiting the synthesis of deoxynivalenol (DON) in Fusarium graminearum, rather than by enhancing the wheat resistance response. MeJA treatment reduced the infection by wild-type F. graminearum in wheat coleoptiles, but exhibited no significant influence on that of the DON-deficient mutant strain (∆Tri5). The production of DON in F. graminearum was significantly inhibited both in vitro and in planta. MeJA affected the expression of genes related to DON biosynthesis, without influencing the formation of toxisomes as observed under microscopic analysis. Exogenous MeJA demonstrated a limited impact on the early genes of plant jasmonic acid signalling pathway, in contrast to the wild-type pathogen strain, which induced the upregulation of these genes. The expression levels of defence marker genes induced by MeJA were notably lower compared to those induced by the pathogen. This study elucidates the molecular mechanisms of MeJA in modulating the wheat-F. graminearum interaction, providing new insights into the development of environmentally friendly strategies against fungi.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14593"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potato NPH3/RPT2-like (NRL) member StNRL-9 interacts with Stphots and negatively regulates late blight resistance. 马铃薯 NPH3/RPT2-like（NRL）成员 StNRL-9 与 Stphots 相互作用并负向调节晚疫病抗性。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14594

Tianyu Lin, Huishan Qiu, Dong Cheng, Qingguo Sun, Lang Liu, Zhendong Tian

{"title":"Potato NPH3/RPT2-like (NRL) member StNRL-9 interacts with Stphots and negatively regulates late blight resistance.","authors":"Tianyu Lin, Huishan Qiu, Dong Cheng, Qingguo Sun, Lang Liu, Zhendong Tian","doi":"10.1111/ppl.14594","DOIUrl":"https://doi.org/10.1111/ppl.14594","url":null,"abstract":"Blue light enhances the susceptibility of Nicotiana benthamiana to Phytophthora infestans, a causative agent of late blight disease. Investigating how blue light affects potato late blight resistance is an interesting aspect of exploring new ways to control late blight disease. Blue light photoreceptor phototropins (phot1, phot2) and their downstream interact protein StNRL1 have been shown to negatively regulate late blight resistance. In order to investigate whether other potato NPH3/RPT2-Like (NRL) family members are involved in regulating late blight resistance, this study focused on the potato NRL proteins containing RxSxS motif at the C-terminus. Another potato NRL protein StNRL-9, containing RxSxS motifs, was found to negatively regulate P. infestans resistance in potato and N. benthamiana. Overexpression of StNRL-9 in potato and N. benthamiana suppresses the accumulation of reactive oxygen species (ROS) and expression of the PTI marker genes NbWRKY7 and NbWRKY8. Similar to StNRL1, StNRL-9 interacts with the blue light receptors Stphot1 and Stphot2 on the cell membrane and could promote the degradation of a positive immune regulator StSWAP70. However StNRL-9 does not inhibit INF1-mediated cell death (ICD), which is different from the StNRL1 that inhibits ICD, indicating that both StNRL1 and StNRL-9 inhibit plant immunity in diverse ways. This study provides valuable information for further exploration of how plant phototropins and NRL family proteins regulate plant immunity.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14594"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In the Spotlight: Journey from a Single Cell to a Flourishing Forest. 聚焦：从单细胞到繁茂森林的旅程。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14609

Haoran Peng

引用次数: 0

Fighting to thrive via plant growth regulators: Green chemical strategies for drought stress tolerance. 通过植物生长调节剂茁壮成长：抗旱的绿色化学策略

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14605

Ali Raza, Savita Bhardwaj, Md Atikur Rahman, Pedro García-Caparrós, Rhys G R Copeland, Sidra Charagh, Rosa M Rivero, Subramaniam Gopalakrishnan, Francisco J Corpas, Kadambot H M Siddique, Zhangli Hu

{"title":"Fighting to thrive via plant growth regulators: Green chemical strategies for drought stress tolerance.","authors":"Ali Raza, Savita Bhardwaj, Md Atikur Rahman, Pedro García-Caparrós, Rhys G R Copeland, Sidra Charagh, Rosa M Rivero, Subramaniam Gopalakrishnan, Francisco J Corpas, Kadambot H M Siddique, Zhangli Hu","doi":"10.1111/ppl.14605","DOIUrl":"https://doi.org/10.1111/ppl.14605","url":null,"abstract":"As global climate change intensifies, the occurrence and severity of various abiotic stresses will significantly threaten plant health and productivity. Drought stress (DS) is a formidable obstacle, disrupting normal plant functions through specific morphological, physiological, biochemical, and molecular mechanisms. Understanding how plants navigate DS is paramount to mitigating its adverse effects. In response to DS, plants synthesize or accumulate various plant growth regulators (PGRs), including phytohormones, neurotransmitters, gasotransmitters, and polyamines, which present promising sustainable green chemical strategies to adapt or tolerate stress conditions. These PGRs orchestrate crucial plant structure and function adjustments, activating defense systems and modulating cellular-level responses, transcript levels, transcription factors, metabolic genes, and stress-responsive candidate proteins. However, the efficacy of these molecules in mitigating DS depends on the plant species, applied PGR dose, treatment type, duration of DS exposure, and growth stages. Thus, exploring the integrated impact of PGRs on enhancing plant fitness and DS tolerance is crucial for global food security and sustainable agriculture. This review investigates plant responses to DS, explains the potential of exogenously applied diverse PGRs, dissects the complex chemistry among PGRs, and sheds light on omics approaches for harnessing the molecular basis of DS tolerance. This updated review delivers comprehensive mechanistic insights for leveraging various PGRs to enhance overall plant fitness under DS conditions.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14605"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous 5-aminolevulinic acid promotes carotenoid accumulation in tomato fruits by regulating ethylene biosynthesis and signaling.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14648

Junwen Wang, Yongmei He, Guangzheng Wang, Ruirui Li, Yu Niu, Kai Liu, Jing Zhang, Zhongqi Tang, Jian Lyu, Jianming Xie, Yue Wu, Jihua Yu

{"title":"Exogenous 5-aminolevulinic acid promotes carotenoid accumulation in tomato fruits by regulating ethylene biosynthesis and signaling.","authors":"Junwen Wang, Yongmei He, Guangzheng Wang, Ruirui Li, Yu Niu, Kai Liu, Jing Zhang, Zhongqi Tang, Jian Lyu, Jianming Xie, Yue Wu, Jihua Yu","doi":"10.1111/ppl.14648","DOIUrl":"https://doi.org/10.1111/ppl.14648","url":null,"abstract":"5-Aminolevulinic acid (ALA) can not only improve fruit yield and quality, but also increase the lycopene content in tomato fruits. Furthermore, ALA has been shown to promote system-2 ethylene production in tomato fruits. However, the specific interactions between ALA and ethylene during fruit ripening remain unclear. In this study, we treated tomato fruits with ALA, 1-aminocyclopropane-1-carboxylic acid (ACC), aminooxyacetic acid (AOA) + AgNO3, and AOA + AgNO3 + ALA and analyzed ethylene emissions, carotenoid contents, and the relative gene expression levels related to fruit ripening, carotenoid contents, ethylene synthesis, and signal transduction. The ALA treatment significantly enhanced ethylene bursts and carotenoid accumulation, and significantly upregulated the expression of ethylene and carotenoid-related genes, such as SlACS2, SlACS4, SlACO1, SlPSY1, and SlPDS. We also observed that the gene expression levels associated with carotenoid synthesis were downregulated in fruits treated with a combination of ethylene inhibitors (AOA + AgNO3). However, there was a significant upregulation in the gene expression levels associated with carotenoid synthesis and an increase in carotenoid content when fruits were treated with AOA + AgNO3 + ALA. After silencing SlACO1 expression, the total carotenoid content and SlPSY1 expression decreased significantly, while this effect was reversed after exogenous application of ALA. These results indicated that ALA promotes carotenoid accumulation in tomato fruits by promoting ethylene biosynthesis. In conclusion, our results highlighted the role of ALA in promoting carotenoid accumulation and ripening in tomato fruits by regulating ethylene synthesis, thereby providing a novel strategy for improving fruit quality.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14648"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-Wide Identification and Salt Stress Response Analysis of the MADS-box Transcription Factors in Sugar Beet.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.70001

Jiahui Cao, Yongyong Gong, Mingyang Zou, Haiying Li, Sixue Chen, Chunquan Ma

{"title":"Genome-Wide Identification and Salt Stress Response Analysis of the MADS-box Transcription Factors in Sugar Beet.","authors":"Jiahui Cao, Yongyong Gong, Mingyang Zou, Haiying Li, Sixue Chen, Chunquan Ma","doi":"10.1111/ppl.70001","DOIUrl":"https://doi.org/10.1111/ppl.70001","url":null,"abstract":"The abiotic stress response and plant development are significantly influenced by MADS-box transcription factors. Nonetheless, the functions of the MADS-box genes in sugar beet stress response are very limited. Here, the sugar beet MADS-box transcription factor gene family was analyzed in sugar beet. The entire genome survey yielded 48 MADS-box genes, which were categorized as type I (Mα, Mβ, and Mγ) and type II (MIKC*, Bs, B, AGL15/AGL18, SVP, FLC, ANR1, SOC1, C/D, Bs, AGL13, E, and A). Five pairs of BvMADS-box genes were fragment duplicated, according to a collinear analysis. A total of fifteen conserved motifs were found, with 1-6 motifs found in each BvMADS-box. Most BvMADS-box genes were expressed more when exposed to salt stress. Among them, the salt-responsive gene BvMADS-box38 is located in the nucleus, as indicated by subcellular localization analysis. Protein interaction network analysis indicated that BvMADS-box proteins were mainly involved in the regulation of the plant flowering process, ABA signal transduction, and stress response. The results of BvMADS-box genes will springboard further studies of their detailed biological functions and inform molecular breeding efforts toward improving sugar beet quality, yield and stress tolerance.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e70001"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-aminobutyric acid does not induce defenses or increase Norway spruce resistance to the bluestain fungus Grosmannia penicillata.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.70009

Ngan Bao Huynh, Paal Krokene, Line Nybakken, Vytautas Čėsna, Melissa H Mageroy

{"title":"β-aminobutyric acid does not induce defenses or increase Norway spruce resistance to the bluestain fungus Grosmannia penicillata.","authors":"Ngan Bao Huynh, Paal Krokene, Line Nybakken, Vytautas Čėsna, Melissa H Mageroy","doi":"10.1111/ppl.70009","DOIUrl":"10.1111/ppl.70009","url":null,"abstract":"Priming of Norway spruce (Picea abies) inducible defenses is a promising way to protect young trees from herbivores and pathogens. Methyl jasmonate (MeJA) application is known to induce and potentially prime Norway spruce defenses but may also reduce plant growth. Therefore, we tested β-aminobutyric acid (BABA) as an alternative priming chemical to enhance spruce resistance, using 2-year-old Norway spruce plants. We compared inducible defense responses, i.e. traumatic resin duct formation and accumulation of defensive metabolites, in bark and xylem tissues of BABA- or MeJA-treated plants before and after wounding. We also evaluated the effect of these chemical treatments on Norway spruce resistance to the pathogenic bluestain fungus Grosmania penicilliata. BABA did not induce defense responses or pathogen resistance, it even reduced concentrations of total terpenes in the treated plants. In contrast, MeJA induced traumatic resin duct formation, accumulation of flavonoids, pathogen resistance, and did not affect plant growth. For the first time, flavan-3-ols (catechins) were shown to have a primed response to MeJA treatment in Norway spruce. Our results indicated that BABA is not a suitable alternative priming chemical to MeJA in Norway spruce.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e70009"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11645543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modern Plant Breeding for Achieving Global Food Security.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.70014

Sajid Fiaz, Muhammad Uzair, Baohong Zhang

引用次数: 0