Physiologia plantarum最新文献_第9页

Exploring Multi-Omics Tools and Their Advancement to Study Drought Stress Responses. 干旱胁迫响应的多组学研究进展

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70520

Kaberi Sonowal, Sonal Sharma, Gokul Anil Kumar, Vaishali Gupta, Gulshan Kumar, Prafull Salvi

{"title":"Exploring Multi-Omics Tools and Their Advancement to Study Drought Stress Responses.","authors":"Kaberi Sonowal, Sonal Sharma, Gokul Anil Kumar, Vaishali Gupta, Gulshan Kumar, Prafull Salvi","doi":"10.1111/ppl.70520","DOIUrl":"https://doi.org/10.1111/ppl.70520","url":null,"abstract":"Climate change poses significant threats to optimal crop production, with drought emerging as a major challenge impacting food security. To meet the demands of a growing population under increasingly variable environmental conditions, it is central to comprehend plant responses to stress. Recent advancements in omics technologies have transformed our understanding of drought-responsive genes, associated networks and pathways at the genome-wide level. These tools enable comprehensive insights into the genetic and molecular mechanisms governing drought response and help us to identify critical genes, regulatory networks, and stress response pathways. This comprehensive review explores the potential of multi-omics approaches in plant functional genomics, aiming to provide a holistic view of drought tolerance mechanisms by integrating data from diverse omics disciplines. By unravelling the complexities of gene regulation, protein dynamics, and metabolic pathways, these integrative tools offer a path toward precise crop improvement strategies. Further, we have also discussed the challenges that persist in integrating these approaches to develop high-yielding, drought-resilient crop varieties. Overall, this article addresses the challenges of utilizing a multi-omics strategy to accelerate targeted crop improvement for drought stress response, and how this approach can help ensure global food security.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70520"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salicylic Acid Biosynthesis via PdCBP60E Confers Anoplophora glabripennis Resistance in Populus deltoides 'Shalinyang'. PdCBP60E生物合成水杨酸对沙林阳三角杨光肩天牛的抗性

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70564

Chenxing He, Ziyi Wang, Wenshan Gao, Chuangjun Xu, Meiying Liu, Jianrong Wei, Jianfeng Liu

{"title":"Salicylic Acid Biosynthesis via PdCBP60E Confers Anoplophora glabripennis Resistance in Populus deltoides 'Shalinyang'.","authors":"Chenxing He, Ziyi Wang, Wenshan Gao, Chuangjun Xu, Meiying Liu, Jianrong Wei, Jianfeng Liu","doi":"10.1111/ppl.70564","DOIUrl":"https://doi.org/10.1111/ppl.70564","url":null,"abstract":"Anoplophora glabriformis (ALB) is one of the most destructive wood-boring insects attacking poplars. Populus deltoides 'Shalinyang' (PdS) is an ALB-resistant species, but the specific molecular mechanisms of ALB resistance are unclear. Here, metabolomics showed that salicylic acid (SA) content increased significantly after ALB infection, and exogenous SA also increased the activity of defense enzymes in PdS. Therefore, the PdCBP60E gene related to SA synthesis was obtained from the PdS. The expression of the upstream PdPAD4 and downstream PdICS1 genes of PdCBP60E increased by 152.48% and 371.26% with ALB infestation, respectively. The SA content, CAT, SOD, and POD activities in transgenic Arabidopsis thaliana increased by 211.3%, 48.3%, 21.3% and 98.0%, respectively. However, VIGS-mediated silencing of PdCBP60E resulted in a 47.4% reduction in PdCBP60E transcript levels and a 41.6% decrease in SA content, respectively. Concurrently, this suppression induced regulatory changes within the SA pathway, characterized by increased PdPAD4 expression and decreased PdICS1 expression. Insect resistance assays further revealed that ALB adults displayed a significantly stronger feeding preference for PdS-silenced plants, with stem damage severity increasing by 193.9%. Additionally, larvae feeding on silenced plants exhibited 28.2% greater biomass accumulation, coupled with elevated activities of digestive enzymes (pectase: increased 78.7%; cellulase: increased 75.3%) and peroxidase (POD: increased 69.1%) compared to those on control plants. Overall, we obtained evidence that PdCBP60E was a positive regulator in ALB resistance improvement in PdS, providing comprehensive insights into the poplar defense system to pest infestation.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70564"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seaweed-Derived Oligosaccharides Confer Broad-Spectrum Antiviral Defenses in Tomato and Arabidopsis Plants. 海藻衍生的低聚糖在番茄和拟南芥植物中具有广谱抗病毒防御作用。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70562

Abhisha Roy, Anish Tamang, Mohit Kumar Swarnkar, T R Girish, Sri Sailaja Nori, Shrikumar Surya Narayan, Saikat Bhattacharjee, Vipin Hallan

{"title":"Seaweed-Derived Oligosaccharides Confer Broad-Spectrum Antiviral Defenses in Tomato and Arabidopsis Plants.","authors":"Abhisha Roy, Anish Tamang, Mohit Kumar Swarnkar, T R Girish, Sri Sailaja Nori, Shrikumar Surya Narayan, Saikat Bhattacharjee, Vipin Hallan","doi":"10.1111/ppl.70562","DOIUrl":"https://doi.org/10.1111/ppl.70562","url":null,"abstract":"Seaweed extracts (SWEs), particularly those derived from red algae, are increasingly recognized as sustainable, eco-friendly alternatives to synthetic agrochemicals. AG Fort, a commercial formulation comprising derivatized low molecular weight oligosaccharides from Kappaphycus alvarezii, was previously reported to prime antibacterial immunity in Arabidopsis thaliana. In this study, we evaluated the antiviral efficacy of the seaweed-based formulation AG Fort against Tomato leaf curl Palampur virus (ToLCPalV), a bipartite begomovirus that causes severe disease in tomato crops across northern India and parts of the Middle East and has a wide natural host range. Symptoms of ToLCPalV infection include extensive leaf curling, chlorosis, and stunted growth, leading to significant yield losses. With current control strategies relying heavily on hazardous pesticides targeting insect vectors, there is an urgent need for safer alternatives. We demonstrate that prophylactic application of AG Fort, either via foliar spray or transient root dipping, significantly reduces disease incidence and symptom severity in the virus-challenged tomato plants. Treated plants exhibited upregulated expression of defense-related genes, increased phytohormone levels, decreased accumulation of viral replicative intermediates, and reduced levels of viral transcripts. Notably, similar antiviral protection was also observed in AG Fort-treated Arabidopsis plants challenged with Cucumber mosaic virus (CMV), suggesting a broad-spectrum antiviral potential. Together, our findings highlight seaweed-derived oligosaccharides as sustainable, seaweed-based crop protection agents capable of mitigating viral infections in Arabidopsis thaliana and tomato, with broader crop applicability indicated in manufacturer data as part of integrated disease management against multiple plant viruses.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70562"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Interplay Between Gama-Aminobutyric Acid and Hydrogen Sulfide and Their Potential Role in Mitigating Cadmium Toxicity in Rice Seedlings. γ -氨基丁酸与硫化氢的相互作用及其在水稻幼苗镉中毒中的潜在作用

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70490

Saumya Jaiswal, Samiksha Singh, Ravi Gupta, Durgesh Kumar Tripathi, Vijay Pratap Singh

{"title":"An Interplay Between Gama-Aminobutyric Acid and Hydrogen Sulfide and Their Potential Role in Mitigating Cadmium Toxicity in Rice Seedlings.","authors":"Saumya Jaiswal, Samiksha Singh, Ravi Gupta, Durgesh Kumar Tripathi, Vijay Pratap Singh","doi":"10.1111/ppl.70490","DOIUrl":"10.1111/ppl.70490","url":null,"abstract":"Both hydrogen sulfide (H2S) and gamma-aminobutyric acid (GABA) are known to regulate antioxidative metabolic pathways for developing stress resilience in plants. However, it is unknown whether they work together or exhibit independent signaling in maintaining ROS homeostasis during cadmium (Cd) stress. Therefore, this study was undertaken to investigate the potential implications of GABA and H2S signaling in regulating Cd stress in rice seedlings. The results show that the exposure to Cd stress affects photosynthesis and nitrogen assimilation, increases ROS accumulation, weakens the antioxidant system, and disturbs thiol metabolism in rice seedlings. Cd stress also stimulated aerenchyma formation, which was further enhanced by GABA application, while H2S did not exhibit any significant effect. GABA treatment also decreased the Cd-induced tannin deposition in roots. Moreover, exogenous GABA and H2S application also enhanced plant growth and chlorophyll content, and restored photosynthesis and nitrogen homeostasis by stabilizing NR, NiR, and GS/GOGAT activities under Cd stress. Further, thiol metabolism was also strengthened, aiding in redox balance restoration under Cd stress. GABA was more effective than H2S in mitigating Cd stress. Moreover, both GABA and H2S were capable of mitigating Cd stress, and our results revealed that GABA seems to be a downstream component of H2S signaling. The results of this study highlight a hopeful and sustainable approach for refining crop resilience to heavy metal stress and Cd stress in particular.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70490"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meta-Analysis of Iron Excess Stress in Rice: Genes and Mechanisms of Tolerance to Acidic Soil. 水稻铁胁迫的荟萃分析：耐酸性土壤的基因和机制。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70473

Divya Gupta, Sanjib Kumar Panda, Petra Bauer

{"title":"Meta-Analysis of Iron Excess Stress in Rice: Genes and Mechanisms of Tolerance to Acidic Soil.","authors":"Divya Gupta, Sanjib Kumar Panda, Petra Bauer","doi":"10.1111/ppl.70473","DOIUrl":"https://doi.org/10.1111/ppl.70473","url":null,"abstract":"Iron toxicity, predominantly stemming from excessive levels of ferrous iron (Fe2+) in acidic soils, poses a considerable challenge for crop production. Hypoxic conditions induced by waterlogging can exacerbate Fe2+ availability, which significantly impacts the cultivation and productivity of rice (Oryza sativa), a staple food for millions worldwide. In several regions across South America, Africa, and Asia, the prevalence of acidic soils results in elevated Fe2+ levels leading to iron toxicity, thereby hindering rice yield. Some regional rice varieties demonstrate a notable adaptation to high iron conditions, offering insights into the tolerance mechanisms through comparative physiology and transcriptomic studies. This review synthesizes the various strategies employed by rice plants to mitigate iron toxicity stress, with a focus on the regulation of essential genes and genetic pathways associated with iron transport and homeostasis. We place particular emphasis on the co-expression networks and predicted subcellular localization of the proteins encoded by these genes. A meta-analysis of differential gene expression data gathered from studies involving six distinct rice lines-either tolerant or sensitive-reveals significant influences of plant genotype, developmental stage, and treatment type on the expression patterns, leading to the identification of robust marker genes associated with the iron excess response. Our comprehensive literature review uncovers several critical knowledge gaps, establishing a framework for developing novel approaches aimed at elucidating the molecular mechanisms underpinning iron stress tolerance. These insights are vital for enhancing rice yield in iron-rich, acidic soils, ultimately contributing to improved food security in affected regions.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70473"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12391641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical and Biophysical Investigation of a Calmodulin-Like Protein From Glycine max Delineates Its Role as a Calcium Sensor During Herbivory. 从甘氨酸中提取的钙调素样蛋白的生化和生物物理研究描述了它在草食过程中作为钙传感器的作用。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70480

Manisha Yadav, Indrakant Kumar Singh, Md Imtaiyaz Hassan, Archana Singh

{"title":"Biochemical and Biophysical Investigation of a Calmodulin-Like Protein From Glycine max Delineates Its Role as a Calcium Sensor During Herbivory.","authors":"Manisha Yadav, Indrakant Kumar Singh, Md Imtaiyaz Hassan, Archana Singh","doi":"10.1111/ppl.70480","DOIUrl":"https://doi.org/10.1111/ppl.70480","url":null,"abstract":"The environment surrounding plants is far from stable, compelling the plant to perceive and adapt to numerous biotic and abiotic constraints, including insect attacks. The perception of a feeding insect typically entails the identification of herbivore-associated molecular patterns causing a sequential increase in cytosolic Ca2+ levels. Calmodulin-like proteins (CMLs) are Ca2+ sensor proteins with conserved EF-hands, which decode Ca2+ signals to generate a stress-specific response. Although few of the CMLs have been investigated and their role in plant defence has been deciphered in model plants, the role of CMLs as a Ca2+ sensor protein and their interaction mechanism with calcium and downstream targets remains poorly understood in leguminous crop plants. Herein, we demonstrated the presence of a Ca2+ signature in Glycine max during herbivory. Gene expression analysis of GmCML77 (a member of the CML family) indicated its upregulation during S. litura infestation. Phylogenetic analysis and in silico studies predicted GmCML77 as a Ca2+ binding protein. Employing computational modelling and MD simulations, we showed that GmCML77 has predominantly α-helical conformation with 3 functional Ca2+ binding loops. Also, Ca2+ binding initiates an expansion of tertiary structure, leading to the exposure of hydrophobic residues that may be implicated in its interaction with target proteins. Moreover, gel shift assay and CD spectroscopy results confirmed the Ca2+ binding ability of GmCML77. Our study demonstrated that GmCML77 is a functional calcium-binding protein, which exhibits conformational changes on Ca2+ binding and acts as a Ca2+ sensor during insect infestation.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70480"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Insights of Phytohormone-Like Small Biomolecules for Microalgae-Mediated Heavy Metal Bioremediation. 植物激素类小生物分子在微藻介导重金属生物修复中的应用。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70506

Kabari Krishna Borah, Prabhakar Semwal, Vinod Kumar, Mikhail S Vlaskin, Manisha Nanda

{"title":"Molecular Insights of Phytohormone-Like Small Biomolecules for Microalgae-Mediated Heavy Metal Bioremediation.","authors":"Kabari Krishna Borah, Prabhakar Semwal, Vinod Kumar, Mikhail S Vlaskin, Manisha Nanda","doi":"10.1111/ppl.70506","DOIUrl":"https://doi.org/10.1111/ppl.70506","url":null,"abstract":"Environmental sustainability is seriously threatened by the discharge of wastewater containing hazardous heavy metals (such as Cr, Cd, As, Hg, etc.). The utilization of microalgae has recently come to light as a viable, environmentally acceptable method for removing heavy metals from contaminated sites. Certain small biomolecules that resemble phytohormones can be beneficial in microalgal biotechnology as they control biological processes and signal transduction to increase stress tolerance and simultaneously upregulate the production of beneficial metabolites. As a result, they make good candidates for bioremediation and an effective vector for removing heavy metal pollutants from the environment. Melatonin, γ-aminobutyric acid (GABA), polyamines, and glycine-betaine are small biomolecules that act as signaling molecules or regulators in microalgae. They play crucial roles in controlling cell development, metabolism, stress resistance, heavy metal accumulation, and redox homeostasis. The potential of phytohormone-like small biomolecules and their incorporation into microalgal systems has been immensely explored by researchers across the globe. However, most studies have reported compromised photosynthetic efficiency in the targeted microalgae and repressed metabolite accumulation. There is then the need for developing cultivation methods without compromising cell viability and photosynthetic efficiency. Therefore, there is a greater need to understand the underlying mechanisms controlling cell proliferation and heavy metal bioaccumulation through the application of phytohormone-like small biomolecules. The current review aims to explore the efficacy of phytohormone-like small biomolecules in the context of microalgal bioremediation of heavy metals alongside the enhancement of various algal metabolites.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70506"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wheat Maintains Stem Water Potential During Drought Stress Despite Declining Osmotic Potential. 干旱胁迫下小麦在渗透势下降的情况下保持茎秆水势。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70513

Sarah Verbeke, Carmen María Padilla-Díaz, Sarah Lievens, Helena Faveere, Stan Goethals, Zoë Vandekerckhove, Kim Vandewoestijne, Kathy Steppe

{"title":"Wheat Maintains Stem Water Potential During Drought Stress Despite Declining Osmotic Potential.","authors":"Sarah Verbeke, Carmen María Padilla-Díaz, Sarah Lievens, Helena Faveere, Stan Goethals, Zoë Vandekerckhove, Kim Vandewoestijne, Kathy Steppe","doi":"10.1111/ppl.70513","DOIUrl":"https://doi.org/10.1111/ppl.70513","url":null,"abstract":"Plant water potential is one of the most frequently measured variables of plant water status. Stem water potential, often approximated by wrapping the leaves, is assumed to be more stable and a better measure of drought stress than leaf water potential. In wheat (Triticum aestivum L.), the stems cannot be seen as merely water transporting organs; rather, they store high amounts of osmotically active carbohydrates, which are postulated to affect water uptake and storage. This study compared the true stem water potential with the wrapped leaf water potential. One drought-sensitive cultivar (Viking) and two resistant cultivars (Impala and Servus) were subjected to different levels of drought stress. Osmotic potential and water content were also measured to study and compare the hydraulic responses to drought stress in the different cultivars. We found that the wrapped leaf water potential does not match the stem water potential in wheat. Instead, wheat maintains its stem water potential during drought stress. Despite the low osmotic potential in the stem parenchyma, the water potential in the stem xylem did not decline as drought stress progressed. This paradox can be explained by recent findings that not only water potential-driven flow, but also turgor-driven flow occurs in wheat stems. This hypothesis suggests that the carbohydrates in stem parenchyma induce an influx of water, but that this water is transported back out under a hydrostatic gradient, redirecting the water to the developing ear.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70513"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin's Role in Enhancing Waterlogging Tolerance in Plants: Current Understanding and Future Directions. 褪黑素在提高植物耐涝能力中的作用：目前的认识和未来的方向。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70499

Mohammad Shah Jahan, Md Mahadi Hasan, Abu Bakar Siddique, Saeedeh Zarbakhsh, Maha M Hamada, Md Arif Hussain, Dilfuza Jabborova, Francisco J Corpas

{"title":"Melatonin's Role in Enhancing Waterlogging Tolerance in Plants: Current Understanding and Future Directions.","authors":"Mohammad Shah Jahan, Md Mahadi Hasan, Abu Bakar Siddique, Saeedeh Zarbakhsh, Maha M Hamada, Md Arif Hussain, Dilfuza Jabborova, Francisco J Corpas","doi":"10.1111/ppl.70499","DOIUrl":"https://doi.org/10.1111/ppl.70499","url":null,"abstract":"Waterlogging, increasingly intensified by climate change, limits oxygen availability in the root zone, disrupting carbon and sugar metabolism, leading to energy deficits and oxidative stress that ultimately impair plant growth and productivity. Melatonin, a versatile signaling molecule, mitigates waterlogging-induced stress by enhancing anaerobic respiration and fermentation under oxygen-deprived conditions, upregulating stress-responsive genes, and restoring energy balance through optimized sugar metabolism. It also reduces oxidative damage by strengthening the antioxidant defense system and further improves stress tolerance by modulating phytohormone signaling and influencing rhizosphere microbiome dynamics. However, while melatonin's role in other abiotic stresses is well documented, its molecular mechanisms in conferring waterlogging tolerance, particularly the regulation of transcriptional and epigenetic processes and plant-microbe interaction, remain underexplored. This review synthesizes current knowledge on melatonin's protective mechanisms against waterlogging stress, uniquely integrating insights across physiological, molecular, and ecological dimensions. It addresses a critical research gap by highlighting the underexplored interplay between melatonin and waterlogging-specific responses, offering a novel perspective on its multifaceted roles in plant adaptation. Future research should prioritize elucidating melatonin's influence on transcriptional regulation, epigenetic reprogramming, and plant-microbiome interactions under waterlogged conditions. Moreover, translating these insights into practical, melatonin-based agricultural strategies is essential for developing waterlogging-resilient crops and promoting sustainable farming systems in vulnerable regions.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70499"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shoot Nitrate Status Regulates Arabidopsis Shoot Growth and Systemic Transcriptional Responses via Shoot Adenosine Phosphate-Isopentenyltransferase 3. 芽部硝酸盐状态通过芽部磷酸腺苷-异戊烯基转移酶3调控拟南芥芽部生长和系统转录反应。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70542

Kota Monden, Takamasa Suzuki, Mikiko Kojima, Yumiko Takebayashi, Daisuke Sugiura, Tsuyoshi Nakagawa, Hitoshi Sakakibara, Takushi Hachiya

{"title":"Shoot Nitrate Status Regulates Arabidopsis Shoot Growth and Systemic Transcriptional Responses via Shoot Adenosine Phosphate-Isopentenyltransferase 3.","authors":"Kota Monden, Takamasa Suzuki, Mikiko Kojima, Yumiko Takebayashi, Daisuke Sugiura, Tsuyoshi Nakagawa, Hitoshi Sakakibara, Takushi Hachiya","doi":"10.1111/ppl.70542","DOIUrl":"10.1111/ppl.70542","url":null,"abstract":"Plants systemically regulate growth and gene expression according to their internal nitrate status. Our previous study reported that shoot nitrate accumulation increases shoot expression of adenosine phosphate-ISOPENTENYLTRANSFERASE 3 (IPT3) and shoot levels of N6-(Δ2-isopentenyl) adenine (iP)-type cytokinins (CKs). IPT3 expression is localized in the phloem, and iP-type CKs, which are synthesized by IPT3, are phloem-mobile. As CKs are a class of phytohormones that control growth and genome-wide gene expression, shoot-expressed IPT3 may mediate the systemic regulation of growth and transcriptomic responses to shoot nitrate status. To examine this, we developed a novel system to manipulate nitrate levels and IPT3 expression in a shoot-specific manner and performed growth analysis, CK determination, and RNA-sequencing. Our results demonstrated that shoot nitrate accumulation significantly promoted shoot growth and elevated shoot concentrations of iP ribotides, iP-7-N-glucoside, and iP-9-N-glucoside through the action of shoot IPT3. Transcriptomic responses to shoot nitrate accumulation were largely tuned by shoot IPT3, with opposite effects in shoots and roots. Shoot IPT3 amplified shoot responses of nitrate-inducible genes and immune response genes to shoot nitrate accumulation, while it dampened root responses of nitrate transport/assimilation genes. This transcriptomic modulation via shoot IPT3 was accompanied by coherent transcriptional changes in genes encoding mobile peptides and transcriptional repressors. Here we present a novel scheme integrating shoot nitrate status responses with CK signaling.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70542"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0