Yong Qiang Gao, Su Li, Hao Yu Wang, Chuan Jin Shan, Lu Zheng, Ceng Ceng Tian, Jing Kun Zhang, Yi Fan Zhang, Da Li Zeng, Jiu Huang, Ren Fang Shen, Hua Wang, Xiao Fang Zhu
{"title":"一个参与水稻Cd积累的糖基转移酶家族17蛋白的鉴定。","authors":"Yong Qiang Gao, Su Li, Hao Yu Wang, Chuan Jin Shan, Lu Zheng, Ceng Ceng Tian, Jing Kun Zhang, Yi Fan Zhang, Da Li Zeng, Jiu Huang, Ren Fang Shen, Hua Wang, Xiao Fang Zhu","doi":"10.1111/ppl.70323","DOIUrl":null,"url":null,"abstract":"<p><p>Cadmium (Cd) is a heavy metal widely distributed in the environment that poses a significant threat to living organisms because of its strong mobility and toxicity. In this study, a novel gene named Osß-glu from the glycosyltransferase (GT) family was investigated for its role in the Cd stress response in rice. Various experiments were conducted using the japonica cultivar Nipponbare (Nip) and its mutants (Osß-glu-1 and Osß-glu-2). The results showed that Osß-glu was specifically induced by Cd stress rather than by other mineral deficiencies. The Osß-glu mutants exhibited higher sensitivity to Cd stress, with more significant inhibition of root elongation, reduced biomass, and increased Cd accumulation in the roots, shoots, and xylem sap than Nip. Nitro-Blue Tetrazolium (NBT) staining indicated a larger acumulation of superoxide anion in the mutant roots under Cd stress, 3,3'-Diaminobenzidine (DAB) staining showed more pronounced H<sub>2</sub>O<sub>2</sub> accumulation, and Evans Blue staining revealed more dead cells, demonstrating more severe reactive oxygen species (ROS) accumulation and cell damage in mutant roots. Moreover, the mutants had higher hemicellulose content and elevated Cd-binding capacity in the root cell wall, as well as abnormal expression of genes related to Cd absorption and translocation. Overall, multiple lines of evidence suggest that Osß-glu plays a crucial regulatory role in the response of rice to Cd stress, acting as an inhibitor of Cd accumulation. This contributes to a better understanding of the precise control network for Cd tolerance in rice, providing a basis for breeding rice varieties with lower Cd uptake.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70323"},"PeriodicalIF":5.4000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of a Novel Glycosyl Transferase Family 17 Protein Involved in Cd Accumulation in Rice (Oryza sativa).\",\"authors\":\"Yong Qiang Gao, Su Li, Hao Yu Wang, Chuan Jin Shan, Lu Zheng, Ceng Ceng Tian, Jing Kun Zhang, Yi Fan Zhang, Da Li Zeng, Jiu Huang, Ren Fang Shen, Hua Wang, Xiao Fang Zhu\",\"doi\":\"10.1111/ppl.70323\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Cadmium (Cd) is a heavy metal widely distributed in the environment that poses a significant threat to living organisms because of its strong mobility and toxicity. In this study, a novel gene named Osß-glu from the glycosyltransferase (GT) family was investigated for its role in the Cd stress response in rice. Various experiments were conducted using the japonica cultivar Nipponbare (Nip) and its mutants (Osß-glu-1 and Osß-glu-2). The results showed that Osß-glu was specifically induced by Cd stress rather than by other mineral deficiencies. The Osß-glu mutants exhibited higher sensitivity to Cd stress, with more significant inhibition of root elongation, reduced biomass, and increased Cd accumulation in the roots, shoots, and xylem sap than Nip. Nitro-Blue Tetrazolium (NBT) staining indicated a larger acumulation of superoxide anion in the mutant roots under Cd stress, 3,3'-Diaminobenzidine (DAB) staining showed more pronounced H<sub>2</sub>O<sub>2</sub> accumulation, and Evans Blue staining revealed more dead cells, demonstrating more severe reactive oxygen species (ROS) accumulation and cell damage in mutant roots. Moreover, the mutants had higher hemicellulose content and elevated Cd-binding capacity in the root cell wall, as well as abnormal expression of genes related to Cd absorption and translocation. Overall, multiple lines of evidence suggest that Osß-glu plays a crucial regulatory role in the response of rice to Cd stress, acting as an inhibitor of Cd accumulation. This contributes to a better understanding of the precise control network for Cd tolerance in rice, providing a basis for breeding rice varieties with lower Cd uptake.</p>\",\"PeriodicalId\":20164,\"journal\":{\"name\":\"Physiologia plantarum\",\"volume\":\"177 3\",\"pages\":\"e70323\"},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physiologia plantarum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1111/ppl.70323\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70323","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Identification of a Novel Glycosyl Transferase Family 17 Protein Involved in Cd Accumulation in Rice (Oryza sativa).
Cadmium (Cd) is a heavy metal widely distributed in the environment that poses a significant threat to living organisms because of its strong mobility and toxicity. In this study, a novel gene named Osß-glu from the glycosyltransferase (GT) family was investigated for its role in the Cd stress response in rice. Various experiments were conducted using the japonica cultivar Nipponbare (Nip) and its mutants (Osß-glu-1 and Osß-glu-2). The results showed that Osß-glu was specifically induced by Cd stress rather than by other mineral deficiencies. The Osß-glu mutants exhibited higher sensitivity to Cd stress, with more significant inhibition of root elongation, reduced biomass, and increased Cd accumulation in the roots, shoots, and xylem sap than Nip. Nitro-Blue Tetrazolium (NBT) staining indicated a larger acumulation of superoxide anion in the mutant roots under Cd stress, 3,3'-Diaminobenzidine (DAB) staining showed more pronounced H2O2 accumulation, and Evans Blue staining revealed more dead cells, demonstrating more severe reactive oxygen species (ROS) accumulation and cell damage in mutant roots. Moreover, the mutants had higher hemicellulose content and elevated Cd-binding capacity in the root cell wall, as well as abnormal expression of genes related to Cd absorption and translocation. Overall, multiple lines of evidence suggest that Osß-glu plays a crucial regulatory role in the response of rice to Cd stress, acting as an inhibitor of Cd accumulation. This contributes to a better understanding of the precise control network for Cd tolerance in rice, providing a basis for breeding rice varieties with lower Cd uptake.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.