Enhancing UV-B Tolerance in Radish and Mung Bean Plants Using Magnetic Iron Oxide Nanoparticles Foliar Application.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Sunita Kataria, Md Intesaful Haque, Andrej Filacek, Maria Barboricova, Jana Ferencova, Meeta Jain, Anshu Rastogi, Marian Brestic
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引用次数: 0

Abstract

This study investigates the potential of magnetic iron oxide nanoparticles (MIONPs) in mitigating ultraviolet-B radiation (UV-B) induced physiological damage in radish (Raphanus sativus L.) and mung bean (Vigna radiata). Screening of the seed vigour indices identified 1500 mg L-1 MIONPs as the optimal concentration for radish and 100 mg L-1 for mung bean for seed vigour improvement. After the first true leaf appeared (~15 days), plants were exposed to different UV-B intensities: control (UV0, 0 mW m-2), moderate (UV1, 26 mW m-2), and high (UV2, 53 mW m-2), with or without foliar MIONPs application. Results showed that UV-B significantly decreased the net photosynthesis rate (Pn) by 32% in radish and 65% in mung bean after UV2 exposure. Fluorescence parameters, including photosystem II (PSII) efficiency and photosynthetic performance (PIabs), were also impaired by UV-B. UV-B stress led to a decline in plant growth, leaf area, biomass accumulation, and chlorophyll content while increasing antioxidant enzyme activities, flavonoids, anthocyanins, malondialdehyde (MDA), and hydrogen peroxide (H2O2) levels. However, MIONPs treatment enhanced UV-B tolerance by improving pigment content, PSII efficiency, Pn, leaf area, and biomass accumulation while reducing MDA and H2O2 levels, thus improving overall plant physiological health. In the leaf model of energy flux, MIONPs-treated plants showed more active reaction centers and improved electron transport. The OJIP curves differed under UV-B stress, with increasing UV-B stress showing decreased fluorescence intensity at the IP phase. However, plants treated with MIONPs showed higher fluorescence intensity specifically at the IP phase, suggesting their protective effect. The UV sensitivity index (UV-SI) revealed that mung bean is more UV-sensitive than radish. MIONPs treatment increased UV-SI values and enhanced the plant tolerance towards UV-B. The results suggest that the application of MIONPs could improve UV-B resistance in future agricultural practices.

磁性氧化铁纳米颗粒叶面施用增强萝卜和绿豆植物对UV-B的耐受性
本研究探讨了磁性氧化铁纳米颗粒(MIONPs)减轻紫外线b辐射对萝卜(Raphanus sativus L.)和绿豆(Vigna radiata)生理损伤的潜力。种子活力指标筛选结果表明,提高萝卜种子活力的最佳浓度为1500 mg L-1,绿豆种子活力的最佳浓度为100 mg L-1。在第一片真叶出现后(约15天),植物暴露于不同的UV-B强度:对照(UV0, 0 mW m-2),中等(UV1, 26 mW m-2)和高(UV2, 53 mW m-2),有或没有叶面施用MIONPs。结果表明,UV2处理后,萝卜和绿豆的净光合速率分别显著降低32%和65%。荧光参数,包括光系统II (PSII)效率和光合性能(PIabs)也受到UV-B的影响。UV-B胁迫导致植株生长、叶面积、生物量积累和叶绿素含量下降,而抗氧化酶活性、类黄酮、花青素、丙二醛(MDA)和过氧化氢(H2O2)水平升高。然而,MIONPs处理通过提高色素含量、PSII效率、Pn、叶面积和生物量积累,同时降低MDA和H2O2水平,从而提高了植物对UV-B的耐受性,从而改善了植物的整体生理健康。在叶片能量通量模型中,mionps处理的植株反应中心更加活跃,电子传递得到改善。UV-B胁迫下的OJIP曲线不同,UV-B胁迫增加,IP相荧光强度降低。然而,经MIONPs处理的植物在IP期表现出更高的荧光强度,表明其具有保护作用。紫外敏感性指数(UV- si)表明,绿豆比萝卜对紫外线更敏感。MIONPs处理增加了UV-SI值,增强了植物对UV-B的耐受性。结果表明,在未来的农业实践中,应用MIONPs可以提高水稻对UV-B的抗性。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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