辣椒素体外合成及积累的光调控机制研究

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Monisha Arya, Gyanendra Kumar, Parvatam Giridhar
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引用次数: 0

摘要

光是影响植物次生代谢产物生物合成的重要环境因子之一。为了阐明它们的影响,研究了单色LED光谱(黄、蓝、绿、红、白)对辣椒愈伤组织培养24、48和72 h辣椒素类化合物、酚类化合物积累、抗氧化能力和辣椒素类生物合成基因转录变化的调控。与白光相比,蓝光能显著提高辣椒素和二氢辣椒素的含量,分别提高41.43倍和4.36倍。Pearson相关分析证实,总酚类物质、总黄酮与抗氧化能力呈正相关(r = 0.85)。基因表达分析显示,生物合成基因(pAMT、KAS、ACS、ACL、CL、C4H和CS)表达上调,其相关转录因子myb、ERF和jerf表达变化,表明它们在不同单色LED条件下可能对辣椒素的生物合成有调控作用。我们的研究结果表明,定向单色LED灯可以提高C. annuum愈伤组织中生物活性化合物的产量,为大规模生产工业上重要的化合物“辣椒素”提供了有利的策略,可用于制药和食品应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unraveling the Photoregulatory Mechanisms of Capsaicinoids Biosynthesis and Accumulation of Capsaicinoids in Capsicum annuum In Vitro Cultures.

Light is one of the essential environmental factors that significantly influences the biosynthesis of secondary metabolites in plants. To explicate their effect, exposure of monochromatic LED light spectra (yellow, blue, green, red, and white) on the accumulation of capsaicinoids, phenolic compounds, antioxidant capacity, and regulation of transcriptional changes in capsaicinoids biosynthesis genes at 24, 48, and 72 h in chili callus cultures was investigated. Blue light significantly increased capsaicin and dihydrocapsaicin contents by 41.43-fold and 4.36-fold, respectively, compared to white light. Pearson correlation analysis confirmed a strong positive correlation between total phenolics, total flavonoids, and antioxidant capacity (r > 0.85). Gene expression analysis revealed that the biosynthetic genes (pAMT, KAS, ACS, ACL, CL, C4H, and CS) were upregulated, and their associated transcription factors-MYB, ERF, and JERF-showed varied expression, indicating their putative regulatory role in capsaicinoid biosynthesis under different monochromatic LED conditions. Our findings demonstrate that the targeted monochromatic LED lights can enhance bioactive compound production in C. annuum callus cultures, providing a favorable strategy for feasible production of the industrially important compound "capsaicin" on a large scale for pharmaceutical and food applications.

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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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