Physiologia plantarum最新文献_第3页

TaCCS1-B expression modulates copper, enzymatic antioxidants and polyphenols contents and provides abiotic stress tolerance in transgenic Arabidopsis.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14645

Shivi Tyagi, Shumayla, Samar Singh, Ashutosh Pandey, Santosh Kumar Upadhyay

{"title":"TaCCS1-B expression modulates copper, enzymatic antioxidants and polyphenols contents and provides abiotic stress tolerance in transgenic Arabidopsis.","authors":"Shivi Tyagi, Shumayla, Samar Singh, Ashutosh Pandey, Santosh Kumar Upadhyay","doi":"10.1111/ppl.14645","DOIUrl":"https://doi.org/10.1111/ppl.14645","url":null,"abstract":"Abiotic stress, including osmotic and salinity stress, significantly affects plant growth and productivity. Copper chaperone for superoxide dismutase (CCS) is essential for copper homeostasis and oxidative stress management. In this study, we investigated the role of the TaCCS1-B gene of bread wheat in enhancing stress tolerance in yeast and transgenic Arabidopsis. Expression of TaCCS1-B increased abiotic stress tolerance in recombinant yeast cells. Phenotypic analysis of Arabidopsis TaCCS1-B expressing lines demonstrated that they exhibited significantly higher germination rates, increased root length and better growth under osmotic and salinity stress than wild type. Additionally, the transgenic lines exhibited higher copper accumulation and enhanced photosynthetic pigments and proline level, coupled with reduced hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) contents. They also showed higher enzymatic antioxidants' activities, indicating reduced oxidative stress in transgenic lines, resulting in reduced flavonoid content. Gene expression analysis indicated modulated expression of stress-responsive genes in the transgenic lines under stress conditions. These findings suggested the role of TaCCS1-B in enhancing stress tolerance by improving copper homeostasis and regulating key stress-responsive genes. This study highlights the potential of TaCCS1-B for the development of better stress resilience crops, which is critical for sustaining agricultural productivity for food security under adverse environmental conditions.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14645"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grafting with non-suckering rootstock increases drought tolerance in Corylus avellana L. through physiological and biochemical adjustments.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.70003

Amedeo Moine, Walter Chitarra, Luca Nerva, Chiara Agliassa, Giorgio Gambino, Francesca Secchi, Chiara Pagliarani, Paolo Boccacci

{"title":"Grafting with non-suckering rootstock increases drought tolerance in Corylus avellana L. through physiological and biochemical adjustments.","authors":"Amedeo Moine, Walter Chitarra, Luca Nerva, Chiara Agliassa, Giorgio Gambino, Francesca Secchi, Chiara Pagliarani, Paolo Boccacci","doi":"10.1111/ppl.70003","DOIUrl":"10.1111/ppl.70003","url":null,"abstract":"Physiological and molecular mechanisms underpinning plant water stress responses still need deeper investigation. Particularly, the analysis of rootstock-mediated signals represents a complex research field, offering potential applicative perspectives for improving the adaptation of fruit crops to environmental stresses. Nonetheless, fundamental knowledge on this subject needs to be widened, especially in some woody species, including European hazelnut (Corylus avellana L). To fill these gaps, we inspected dynamic changes in gas exchanges and stem water potential of two hazelnut genotypes, the 'San Giovanni' cultivar (SG), the non-suckering rootstock 'Dundee' (D), and their heterograft (SG/D), during a drought stress treatment followed by recovery. Biometric and anatomical traits were measured at the beginning and end of water stress imposition. Additionally, differences in abscisic acid and proline contents were analysed in leaves and roots taken from well-irrigated, stressed and recovered plants, in combination with expression profiles of candidate genes. Grafting with 'Dundee' rootstock positively affected the ability of 'San Giovanni' plants to endure drought by increasing their intrinsic water use efficiency and facilitating post-rehydration recovery. Although anatomical adjustments occurred, we showed that the improved stress adaptation of grafted plants rather depended on biochemical modifications, resulting in increased root proline concentrations and leaf ABA accumulation both during water stress and recovery. We also proved that those metabolic changes were controlled by a differential reprogramming of genes involved in hormone metabolism and stress defence. Grafting with non-suckering rootstocks could therefore represent a promising and environmentally-friendly strategy for improving the adaptability of hazelnut to water deficit.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e70003"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11632140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome and metabolome analyses reveal the mechanisms by which H₂S improves energy and nitrogen metabolism in tall fescue under low-light stress.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.70015

Hanyu Li, Si Long, Yize Yu, Shuqi Ran, Jiongjiong Gong, Tianqi Zhu, Yuefei Xu

{"title":"Transcriptome and metabolome analyses reveal the mechanisms by which H2S improves energy and nitrogen metabolism in tall fescue under low-light stress.","authors":"Hanyu Li, Si Long, Yize Yu, Shuqi Ran, Jiongjiong Gong, Tianqi Zhu, Yuefei Xu","doi":"10.1111/ppl.70015","DOIUrl":"https://doi.org/10.1111/ppl.70015","url":null,"abstract":"Hydrogen sulfide (H2S) functions as a signaling molecule affecting plant growth, development, and stress adaptation. Tall fescue (Festuca arundinacea Schreb.), a bioenergy crop, encounters significant challenges in agricultural production owing to low light by shading. However, the influence of H2S on tall fescue under low light stress (LLS) remains unclear. To examine the role of H2S in acclimation of tall fescue to low light, we conducted combined analyses of physiological traits, metabolomics, and transcriptomics. These results showed that H2S mitigated LLS-induced inhibition of photosynthesis and maintained normal chloroplast ultrastructure by boosting the expression of photosynthesis-related genes, including PsbQ, PsbR, PsaD, PsaK, and PetH, thereby enhancing the synthesis of carbohydrates (sucrose, starch). H2S upregulated the expression of key genes (PFK, PK, IDH, G6PD) connected to glycolysis, the tricarboxylic acid cycle, and the pentose phosphate pathway to promote carbon metabolism and ensure the supply of carbon skeletons and energy required for nitrogen metabolism. H2S application reverted the LLS-induced accumulation of nitrate nitrogen and the changes in the key nitrogen metabolism enzymes glutamate synthase (GOGAT, EC 1.4.1.13), nitrate reductase (NR, EC 1.6.6.1), glutamine synthetase (GS, EC 6.3.1.2), and glutamate dehydrogenase (GDH, EC 1.4.1.2), thus promoting amino acid decomposition to produce proteins involved in nitrogen assimilation and nitrogen use efficiency as well as specialized metabolism. Ultimately, H2S upregulated the C/N ratio of tall fescue, balanced its carbon and nitrogen metabolism, enhanced shade tolerance, and increased biomass. These results provided new insights into enhancing plant resilience under LLS.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e70015"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing light-harvesting chlorophyll a/b-binding proteins for multiple abiotic stress tolerance in Chlamydomonas reinhardtii: Insights from genomic and physiological analysis.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14653

Ali Raza, Yiran Li, Hafiz Muhammad Rizwan, Asadullah Khan, Yuqi Peng, Chunli Guo, Zhangli Hu

{"title":"Harnessing light-harvesting chlorophyll a/b-binding proteins for multiple abiotic stress tolerance in Chlamydomonas reinhardtii: Insights from genomic and physiological analysis.","authors":"Ali Raza, Yiran Li, Hafiz Muhammad Rizwan, Asadullah Khan, Yuqi Peng, Chunli Guo, Zhangli Hu","doi":"10.1111/ppl.14653","DOIUrl":"https://doi.org/10.1111/ppl.14653","url":null,"abstract":"Light-harvesting chlorophyll a/b-binding proteins (LHC) of photosystem II perform key functions in various processes, e.g., photosynthesis, development, and abiotic stress responses. Nonetheless, comprehensive genome-wide investigation of LHC family genes (CrLHCs) has not been well-reported in single-cell alga (Chlamydomonas reinhardtii). Here, we discovered 61 putative CrLHC genes in the C. reinhardtii genome and observed that most genes demonstrate stable exon-intron and motif configurations. We predicted five phytohormones- and six abiotic stress-interrelated cis-regulatory elements in promoter regions of CrLHC. Likewise, 19 miRNAs targeting 42 CrLHC genes from 16 unique families were discovered. Besides, we identified 400 transcription factors from 13 families, including ERF, GATA, CPP, bZIP, C3H, MYB, SBP, Dof, bHLH, C2H2, G2-like, etc. Protein-protein interactions and 3D structures provided insight into CrLHC proteins. Gene ontology and KEGG-based enrichment advocated their role in light responses, photosynthesis, and energy metabolisms. Expression analysis highlighted the shared and unique roles of many CrLHC genes against different abiotic stresses (UV-C, green light, heat, nitric oxide, cadmium, nitrogen starvation, and salinity). Under salinity stress, antioxidant enzyme activity, reactive oxygen species markers, photosynthesis-related traits and pigments were significantly affected. Briefly, this comprehensive genomic and physiological study shed light on the impact of CrLHC genes in abiotic stress tolerance and set the path for future genetic engineering experiments.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14653"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous melatonin improves drought stress tolerance via regulating tryptophan metabolism and flavonoid biosynthesis pathways in wheat.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.70006

Jingyu Li, Yuanxin Li, Mengxue Du, Dongtian Zang, Qingmei Men, Peisen Su, Shangjing Guo

{"title":"Exogenous melatonin improves drought stress tolerance via regulating tryptophan metabolism and flavonoid biosynthesis pathways in wheat.","authors":"Jingyu Li, Yuanxin Li, Mengxue Du, Dongtian Zang, Qingmei Men, Peisen Su, Shangjing Guo","doi":"10.1111/ppl.70006","DOIUrl":"https://doi.org/10.1111/ppl.70006","url":null,"abstract":"Melatonin (MT) serves an indispensable function in plant development and their response to abiotic stress. Although numerous drought-tolerance genes have been ascertained in wheat, further investigation into the molecular pathways controlling drought stress tolerance remains necessary. In this investigation, it was observed that MT treatment markedly enhanced drought resistance in wheat by diminishing malondialdehyde (MDA) levels and augmenting the activity of antioxidant enzymes POD, APX, and CAT compared to untreated control plants. Transcriptomic analysis disclosed that melatonin treatment activated the tryptophan metabolism and flavonoid biosynthesis pathways. Furthermore, quantitative reverse transcription PCR (qRT-PCR) outcomes validated that the expression trends of these differentially expressed genes aligned with the transcriptomic data. Metabolomic profiling identified alterations in the abundance of several metabolites, including tryptamine, MT, formylanthranilate, 3-hydroxyanthranilate, 6-hydroxymelatonin, naringenin chalcone, astragalin, pinbanksin, and caffeoyl quinic acid. Co-expression analysis suggested that various transcription factors-encompassing AP2/ERF-ERF, WRKY, bZIP, C2H2, bHLH, NAC, and MYB-participated in controlling the differentially expressed genes across multiple pathways. Ultimately, these findings highlight that exogenous MT application bolsters wheat's drought tolerance through the modulation of tryptophan metabolism and flavonoid biosynthesis. These insights provide novel perspectives on the molecular frameworks mediating MT's effect on drought resistance and pinpointing candidate genes for potential genetic enhancement programs in wheat.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e70006"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycosyltransferase-Mediated Modulation of Reactive Oxygen Species Enhances Non-host Resistance to Pst DC3000 in Nicotiana benthamiana.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.70019

Yingjun Liu, Siyi Zhang, Min Sun, Xingqian Hao, Pinyuan Jin, Sheng Luo, Jiao Chen, Ting Zhang, Shating Ge, Huajian Zhang

{"title":"Glycosyltransferase-Mediated Modulation of Reactive Oxygen Species Enhances Non-host Resistance to Pst DC3000 in Nicotiana benthamiana.","authors":"Yingjun Liu, Siyi Zhang, Min Sun, Xingqian Hao, Pinyuan Jin, Sheng Luo, Jiao Chen, Ting Zhang, Shating Ge, Huajian Zhang","doi":"10.1111/ppl.70019","DOIUrl":"https://doi.org/10.1111/ppl.70019","url":null,"abstract":"Non-host resistance (NHR) governs defense responses against a broad range of potential pathogen species in contrast with host resistance. To identify specific genes involved in disease resistance, we used a virus-induced gene-silencing screen in Nicotiana benthamiana and identified glycosyltransferase (NbGT) as an essential component of NHR. NbGT silencing enhanced the hypersensitivity response, reactive oxygen species response, and callose deposition in N. benthamiana, improving its NHR to Pseudomonas syringae pv. tomato (Pst) DC3000. NbGT participated in reactive oxygen species accumulation caused by flg22 rather than coronatine and HrcC of Pst DC3000. Analyses of gene expression and enzyme activity demonstrated that NbGT-silenced plants exhibited enhanced expression and elevated levels of superoxide dismutase, resulting in heightened accumulation of H2O2. In conclusion, NbGT-silencing increases H2O2 accumulation by regulating superoxide dismutase activity during the immune response to flg22, enhancing resistance to Pst DC3000 in N. benthamiana. This research provides novel insights into the role of glycosyltransferases in NHR.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e70019"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in SWEET-mediated sugar partitioning affect photosynthesis performance and plant response to drought. SWEET 介导的糖分配变化会影响光合作用性能和植物对干旱的反应。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14623

Emilie Aubry, Gilles Clément, Elodie Gilbault, Sylvie Dinant, Rozenn Le Hir

{"title":"Changes in SWEET-mediated sugar partitioning affect photosynthesis performance and plant response to drought.","authors":"Emilie Aubry, Gilles Clément, Elodie Gilbault, Sylvie Dinant, Rozenn Le Hir","doi":"10.1111/ppl.14623","DOIUrl":"https://doi.org/10.1111/ppl.14623","url":null,"abstract":"Sugars, produced through photosynthesis, are at the core of all organic compounds synthesized and used for plant growth and their response to environmental changes. Their production, transport, and utilization are highly regulated and integrated throughout the plant life cycle. The maintenance of sugar partitioning between the different subcellular compartments and between cells is important in adjusting the photosynthesis performance and response to abiotic constraints. We investigated the consequences of the disruption of four genes coding for SWEET sugar transporters in Arabidopsis (SWEET11, SWEET12, SWEET16, and SWEET17) on plant photosynthesis and the response to drought. Our results show that mutations in both SWEET11 and SWEET12 genes lead to an increase of cytosolic sugars in mesophyll cells and phloem parenchyma cells, which impacts several photosynthesis-related parameters. Further, our results suggest that in the swt11swt12 double mutant, the sucrose-induced feedback mechanism on stomatal closure is poorly efficient. On the other hand, changes in fructose partitioning in mesophyll and vascular cells, measured in the swt16swt17 double mutant, positively impact gas exchanges, probably through an increased starch synthesis together with higher vacuolar sugar storage. Finally, we propose that the impaired sugar partitioning, rather than the total amount of sugars observed in the quadruple mutant, is responsible for the enhanced sensitivity upon drought. This work highlights the importance of considering SWEET-mediated sugar partitioning rather than global sugar content in photosynthesis performance and plant response to drought. Such knowledge will pave the way to design new strategies to maintain plant productivity in a challenging environment.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14623"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic insights into the multiple stress responses of metabolites in major oilseed crops. 通过代谢组学深入了解主要油籽作物代谢物的多重胁迫反应。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14596

Habtamu Kefale, Jun You, Yanxin Zhang, Sewnet Getahun, Muez Berhe, Ahmed A Abbas, Chris O Ojiewo, Linhai Wang

{"title":"Metabolomic insights into the multiple stress responses of metabolites in major oilseed crops.","authors":"Habtamu Kefale, Jun You, Yanxin Zhang, Sewnet Getahun, Muez Berhe, Ahmed A Abbas, Chris O Ojiewo, Linhai Wang","doi":"10.1111/ppl.14596","DOIUrl":"10.1111/ppl.14596","url":null,"abstract":"The multidimensional significance of metabolomics has gained increasing attention in oilseeds research and development. Sesame, peanut, soybean, sunflower, rapeseed, and perilla are the most important oilseed crops consumed as vegetable oils worldwide. However, multiple biotic and abiotic stressors affect metabolites essential for plant growth, development, and ecological adaptation, resulting in reduced productivity and quality. Stressors can result in dynamic changes in oilseed crops' overall performance, leading to changes in primary (ex: saccharides, lipids, organic acids, amino acids, vitamins, phytohormones, and nucleotides) and secondary (ex: flavonoids, alkaloids, phenolic acids, terpenoids, coumarins, and lignans) major metabolite classes. Those metabolites indicate plant physiological conditions and adaptation strategies to diverse biotic and abiotic stressors. Advancements in targeted and untargeted detection and quantification approaches and technologies aided metabolomics and crop improvement. This review seeks to clarify the metabolomics advancements, significant contributions of metabolites, and specific metabolites that accumulate in reaction to various stressors in oilseed crops. Considering the response of metabolites to multiple stress effects, we compiled comprehensive and combined metabolic biosynthesis pathways for six major classes. Understanding these essential metabolites and pathways can inform molecular breeding strategies to develop resilient oilseed cultivars. Hence, this review highlights metabolomics advancements and metabolites' potential roles in major oilseed crops' biotic and abiotic stress responses.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14596"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic-Engineering Approach to Enhance Vanillin and Phenolic Compounds in Ocimum Sanctum (CIM-Angana) via VpVAN Overexpression.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.70005

Zakir Husain, Sana Khan, Aqib Sarfraz, Zafar Iqbal, Ashish Chandran, Kahkashan Khatoon, Gazala Parween, Farah Deeba, Shama Afroz, Feroz Khan, Ratnasekhar Ch, Laiq Ur Rahman

{"title":"Metabolic-Engineering Approach to Enhance Vanillin and Phenolic Compounds in Ocimum Sanctum (CIM-Angana) via VpVAN Overexpression.","authors":"Zakir Husain, Sana Khan, Aqib Sarfraz, Zafar Iqbal, Ashish Chandran, Kahkashan Khatoon, Gazala Parween, Farah Deeba, Shama Afroz, Feroz Khan, Ratnasekhar Ch, Laiq Ur Rahman","doi":"10.1111/ppl.70005","DOIUrl":"https://doi.org/10.1111/ppl.70005","url":null,"abstract":"Transgenic Ocimum sanctum plants were engineered to produce vanillin by overexpressing the VpVAN gene using Agrobacterium-mediated transformation. Positive transformants developed shoots within 4-5 weeks and were transferred to a root induction medium and four independent transformants with no observed adverse effects were kept for anlysis. Quantitative RT-PCR indicated significantly higher VpVAN expression in transgenic lines AG_3 and AG_1, impacting the phenylpropanoid pathway and phenolic compound accumulation. Molecular docking studies indicated ferulic acid's higher binding affinity to vanillin synthase than eugenol. LC-MS/MS analysis revealed a marked increase in vanillin production in transgenic lines compared to wild type, with AG_3 exhibiting the highest vanillin content (1.98 ± 0.0047 mg/g extract) and AG_1 following (1.49 ± 0.0047 mg/g extract). AG_3 also showed elevated levels of benzoic acid, 4-hydroxy benzyl alcohol, and ferulic acid. This study highlights the potential of metabolic engineering in O. sanctum for enhanced vanillin production, suggesting pathways for large-scale production of natural vanillin and other valuable compounds in transgenic plants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e70005"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diverse functional interactions between ABA and ethylene in plant development and responses to stress.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.70000

Xu-Dong Liu, Yuan-Yuan Zeng, Md Mahadi Hasan, Shantwana Ghimire, Hui Jiang, Shi-Hua Qi, Xue-Qian Tian, Xiang-Wen Fang

{"title":"Diverse functional interactions between ABA and ethylene in plant development and responses to stress.","authors":"Xu-Dong Liu, Yuan-Yuan Zeng, Md Mahadi Hasan, Shantwana Ghimire, Hui Jiang, Shi-Hua Qi, Xue-Qian Tian, Xiang-Wen Fang","doi":"10.1111/ppl.70000","DOIUrl":"https://doi.org/10.1111/ppl.70000","url":null,"abstract":"Abscisic acid (ABA) and ethylene are two essential hormones that play crucial roles throughout the entire plant life cycle and in their tolerance to abiotic or biotic stress. In recent decades, increasing research has revealed that, in addition to their individual roles, these two hormones are more likely to function through their interactions, forming a complex regulatory network. More importantly, their functions change and their interactions vary from synergistic to antagonistic depending on the specific plant organ and development stage, which is less focused, compared and systematically summarized. In this review, we first introduce the general synthesis and action signaling pathways of these two plant hormones individually and their interactions in relation to seed dormancy and germination, primary root growth, shoot development, fruit ripening, leaf senescence and abscission, and stomatal movement regulation under both normal and stress conditions. A better understanding of the complex interactions between ABA and ethylene will enhance our knowledge of how plant hormones regulate development and respond to stress and may facilitate the development of crops with higher yields and greater tolerance to stressful environments through tissue-specific genetic modifications in the future.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e70000"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142838538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0