Physiologia plantarum最新文献_第3页

Mutations of the brassinosteroid biosynthesis gene HvDWARF5 enable balance between semi-dwarfism and maintenance of grain size in barley. 油菜素内酯生物合成基因HvDWARF5的突变使大麦在半矮化和维持籽粒大小之间取得平衡。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70179

Karolina Zolkiewicz, Jana Oklestkova, Beata Chmielewska, Damian Gruszka

{"title":"Mutations of the brassinosteroid biosynthesis gene HvDWARF5 enable balance between semi-dwarfism and maintenance of grain size in barley.","authors":"Karolina Zolkiewicz, Jana Oklestkova, Beata Chmielewska, Damian Gruszka","doi":"10.1111/ppl.70179","DOIUrl":"10.1111/ppl.70179","url":null,"abstract":"Brassinosteroids (BRs) are phytohormones which regulate various developmental processes in plants. They are exceptional phytohormones, as they do not undergo long-distance transport between plant organs. However, knowledge about the function of the enzymes that catalyse BR biosynthesis (particularly its early stages) in cereal crops remains limited. Therefore, this study identifies and analyses the function of the HvDWARF5 (HvDWF5) gene, involved in the early stage of BR biosynthesis in barley (Hordeum vulgare), an important cereal crop, using the TILLING (Targeting Induced Local Lesions IN Genomes) approach. The detailed functional analysis allowed for the identification of various mutations in different gene fragments. The influence of these mutations on plant architecture, reproduction, and yield was characterised. Moreover, effects of the missense and intron retention mutations on sequence and splicing of the HvDWF5 transcript, sequence and predicted structure of the encoded HvDWF5 enzyme, and accumulation of endogenous BR were determined. Some of the barley mutants identified in this study showed semi-dwarfism, a trait of particular importance for cereal breeding and yield. However, unlike other BR mutants in cereals, this did not negatively affect grain size or weight. It indicated that mutations in this gene allow for a balance between plant height reduction and maintenance of grain size. Thus, the results of this study provide a novel insight into the role of the HvDWF5 gene in the BR biosynthesis-dependent regulation of architecture and reproduction of the important cereal crop - barley.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70179"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective bioprocess engineering to enhance omega-6 polyunsaturated fatty acid production from Arthrospira platensis. 有效的生物工艺工程提高了platarthrospira的omega-6多不饱和脂肪酸的产量。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70186

Siddhant Dubey, Reeta Rani Singhania, Praveen Kumar Ramanujam, Chiu-Wen Chen, Cheng-Di Dong, Anil Kumar Patel

{"title":"Effective bioprocess engineering to enhance omega-6 polyunsaturated fatty acid production from Arthrospira platensis.","authors":"Siddhant Dubey, Reeta Rani Singhania, Praveen Kumar Ramanujam, Chiu-Wen Chen, Cheng-Di Dong, Anil Kumar Patel","doi":"10.1111/ppl.70186","DOIUrl":"https://doi.org/10.1111/ppl.70186","url":null,"abstract":"Contrary to the robustness of microbial ω-3 polyunsaturated fatty acid (PUFA) production, the microbial synthesis of ω-6 PUFAs remains challenging. The rising demand for ω-6 PUFAs, especially for pregnancy and infant formulas, calls for scalable and sustainable production methods. Arthrospira platensis, a rarely explored microalgae, shows promise as a platform for producing gamma linoleic acid (GLA) and linolenic acid (LA), key components of ω-6 PUFAs. This study employs a two-phase cultivation approach to enhance ω-6 PUFA production in A. platensis. The initial growth phase was optimized to maximize biomass, followed by a stress-induced phase to boost lipid and ω-6 PUFA accumulation. Notably, ω-6 producing strains like A. platensis are protein-rich and not a high oleaginous species, achieving over 15% total lipid content particularly is significant. Under optimized conditions, a maximum biomass of 4.9 g/L with a productivity rate of (0.233 g/L/day) was obtained at 8 K Lux light irradiance, with 2X nitrogen concentration and 4 mg/L phytohormones. The subsequent stress phase, involving 20 K Lux light, 10 mg/L FeSO4, and 1% glycerol, resulted in a lipid content of 22.8%. This approach led to a 2.4-fold and 1.5-fold increase in microalgal biomass and lipid content respectively. Moreover, C18:2 and C18:3 PUFAs reaching approx. 17.1 ± 0.06% and 24.1 ± 0.07%, respectively. This research promotes microalgae cultivation to meet rising ω-6 PUFA demand, aligning with sustainable development goal 3: Good health and well-being.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70186"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SEEDSTICK Affects Seed Development by Mediating Cytokinin Levels in Cotton. SEEDSTICK 通过调节棉花中的细胞分裂素水平影响种子发育。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70161

Xiaohong Zhang, Yifan Li, Yunjie Ge, Yuhao Mao, Genhai Hu, Qifeng Ma, Eryong Chen

{"title":"SEEDSTICK Affects Seed Development by Mediating Cytokinin Levels in Cotton.","authors":"Xiaohong Zhang, Yifan Li, Yunjie Ge, Yuhao Mao, Genhai Hu, Qifeng Ma, Eryong Chen","doi":"10.1111/ppl.70161","DOIUrl":"https://doi.org/10.1111/ppl.70161","url":null,"abstract":"The SEEDSTICK transcription factor is important for flower and seed development, but the underlying molecular mechanisms remain unclear in cotton. In this study, we identified and cloned two STK homolog genes in upland cotton, an economically valuable cultivated crop. Phylogenetic and sequence analyses showed that the C-terminus of both GhSTKs had a conserved -DJJILHLG amino acid sequence and that GhSTK1 and GhSTK2 were very similar to GaAGL11 and GrAGL11, respectively. Quantitative real-time PCR analysis revealed that both GhSTKs were highly expressed in the ovules, and GUS activity was detected in the style and stigma. Subcellular localization experiments showed that GhSTK1 and GhSTK2 were localized to the nucleus. In Arabidopsis, the overexpression of GhSTK1 or GhSTK2 affected floral organ development and seed formation by increasing the transcript levels of the CKX genes and other genes related to floral development. Silencing both GhSTK1 and GhSTK2 increased the expression of GhFT and GhSHP and led to the earlier appearance of cotton buds. Yeast two-hybrid and bimolecular fluorescence complementation assays indicated that the two GhSTK proteins could interact with the GhSEP3 and GhSEP4 proteins. The present results suggest that GhSTK1 and GhSTK2, which have different sequences and expression patterns, might be functionally redundant and influence the regulation of cotton bud and seed development.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70161"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of small secreted peptide signaling during defense response: insights from vascular and non-vascular plants. 防御反应中小分泌肽信号的比较分析：来自维管植物和非维管植物的见解。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70147

Irina Lyapina, Daria Ganaeva, Eugene A Rogozhin, Ekaterina V Ryabukhina, Dmitry Yu Ryazantsev, Vassili Lazarev, Sabina E Alieva, Anna Mamaeva, Igor Fesenko

{"title":"Comparative analysis of small secreted peptide signaling during defense response: insights from vascular and non-vascular plants.","authors":"Irina Lyapina, Daria Ganaeva, Eugene A Rogozhin, Ekaterina V Ryabukhina, Dmitry Yu Ryazantsev, Vassili Lazarev, Sabina E Alieva, Anna Mamaeva, Igor Fesenko","doi":"10.1111/ppl.70147","DOIUrl":"https://doi.org/10.1111/ppl.70147","url":null,"abstract":"Small secreted peptides (SSPs) play an important role in modulating immune responses in all land plants. However, the evolution of stress peptide signaling in different plant phyla remains poorly understood. Here, we compared the expression of SSP genes in the pathogen-induced transcriptomes of vascular and non-vascular plants. We found 13, 19, 15, and 28 SSP families that were differentially expressed during infection in Physcomitrium patens, Zea mays, Brassica napus, and Solanum tuberosum, respectively. A comparative study of peptide motifs and predicted three-dimensional structures confirmed the similarity of SSPs across the examined plant species. In both vascular and non-vascular plants. However, only the RALF peptide family was differentially regulated under infection. We also found that EPFL peptides, which are involved in growth and development processes in angiosperms, were differentially regulated in P. patens in response to pathogen infection. The search for novel immune-specific peptides revealed a family of PSY-like peptides that are differentially regulated during infection in P. patens. The treatment with synthetic tyrosine-modified and non-modified PSY, and PSY-like peptides, as well as recombinant EPFL and MEG, validated their roles in the immune response and growth regulation. Thus, our study showed the complex nature of SSP signaling and shed light on the regulation of SSPs in different plant lineages during infection.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70147"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening CYP450 genes from Gleditsia japonica Miq. and identifying CYP710A157 and CYP71D752 functions in the catalysis of echinocystic acid and betulin. 筛选 Gleditsia japonica Miq.的 CYP450 基因，确定 CYP710A157 和 CYP71D752 在催化棘囊酸和白桦脂中的功能。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70176

Ziyu Meng, Ming Dong, Changyixin Xiao, Ying Li, Yuqi Zhang, Jiale Cui, Siyao Wang, Peng Li, Dmitry Baleev, Yaguang Zhan, Jing Yin

{"title":"Screening CYP450 genes from Gleditsia japonica Miq. and identifying CYP710A157 and CYP71D752 functions in the catalysis of echinocystic acid and betulin.","authors":"Ziyu Meng, Ming Dong, Changyixin Xiao, Ying Li, Yuqi Zhang, Jiale Cui, Siyao Wang, Peng Li, Dmitry Baleev, Yaguang Zhan, Jing Yin","doi":"10.1111/ppl.70176","DOIUrl":"https://doi.org/10.1111/ppl.70176","url":null,"abstract":"The triterpenes and saponin compounds of Gleditsia japonica Miq. play a key role in the suppression of various human tumor cell lines. Cytochrome P450 monooxygenases (CYP450s) are critical for the triterpene skeleton diversification and functional modification. This study systematically analyzed 104 full-length GjCYP450 genes in G. japonica from northeast China, classifying them into nine clans using bioinformatics. Co-expression modules and response patterns of GjCYP450s with triterpene pathway genes were constructed. Four genes - CYP710A157, CYP714E97, CYP716A377, and CYP71D752 - were selected for functional studies based on their high expression in different tissues of G. japonica and their homology with triterpenoid-related CYP450s in Arabidopsis thaliana. Co-expression of the CYP710A157 gene with the BpY gene (encoding β-amyrin synthase), and CYP71D752 with the BpW gene (encoding lupeol synthase) in tobacco significantly enhanced the catalytic efficiency of echinocystic acid (EA) and betulin (BT) compared to the control, by achieving 10.22-fold and 3.73-fold increases, respectively. Overexpression of CYP710A157 and CYP71D752 in Saccharomyces cerevisiae JWy602 yielded EA and BT at 3.25 mg l-1 and 13.84 mg l-1, respectively, whereas no product accumulation was detected in the control. Additionally, CYP710A157 and CYP714E97 enhanced yeast alkaline tolerance (500 mmol l-1 Na2CO3), while CYP716A377 and CYP71D752 improved their salt tolerance (10% NaCl). We reported the catalytic activity of CYP450 genes responsible for EA and BT synthesis within the CYP710A and CYP71D subfamilies in G. japonica for the first time here. These findings provide valuable genetic resources for plants' triterpene biosynthesis, including ginsenosides, and betulinic acid, and insights into regulating the triterpene metabolic network in G. japonica.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70176"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A suitable strategy to find IAA metabolism mutants. 寻找IAA代谢突变体的合适策略。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70166

Rubén Casanova-Sáez, Aleš Pěnčík, Rafael Muñoz-Viana, Federica Brunoni, Rui Pinto, Ondřej Novák, Karin Ljung, Eduardo Mateo-Bonmatí

{"title":"A suitable strategy to find IAA metabolism mutants.","authors":"Rubén Casanova-Sáez, Aleš Pěnčík, Rafael Muñoz-Viana, Federica Brunoni, Rui Pinto, Ondřej Novák, Karin Ljung, Eduardo Mateo-Bonmatí","doi":"10.1111/ppl.70166","DOIUrl":"10.1111/ppl.70166","url":null,"abstract":"Indole-3-acetic acid (IAA), the most common form of auxin, is involved in a great range of plant physiological processes. IAA is synthesized from the amino acid tryptophan and can be transported and inactivated in a myriad of ways. Despite intense research efforts, there are still dark corners in our comprehension of IAA metabolism and its interplays with other pathways. Genetic screens are a powerful tool for unbiasedly looking for new players in a given biological process. However, pleiotropism of auxin-related phenotypes and indirect effects make it necessary to incorporate additional screening steps to specifically find mutants affected in IAA homeostasis. We previously developed and validated a high-throughput methodology to simultaneously quantify IAA, key precursors, and inactive forms from as little as 10 mg of fresh tissue. We have carried out a genetic screening to identify mutants involved in IAA metabolism. Auxin reporters DR5pro:VENUS and 35Spro:DII-VENUS were EMS-mutagenized and subjected to a parallel morphological and reporter-signal pre-screen. We then obtained the auxin metabolite profile of 325 M3 selected lines and used multivariate data analysis to identify potential IAA-metabolism mutants. To test the screening design, we identified the causal mutations in three of the candidate lines by mapping-by-sequencing: dii365.3, dii571.1 and dr693. These carry new alleles of CYP83A1, MIAO, and SUPERROOT2, respectively, all of which have been previously involved in auxin homeostasis. Our results support the suitability of this approach to find new genes involved in IAA metabolism.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70166"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Auxin biodynamics and its integral role in enhancing plant resilience to environmental cues. 生长素的生物动力学及其在提高植物对环境的适应能力中的重要作用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70165

Muhammad Ali, Linjuan Shi, Muhammad Aamir Khan, Ahmad Ali, Shuai Hu, Jinbo Shen

{"title":"Auxin biodynamics and its integral role in enhancing plant resilience to environmental cues.","authors":"Muhammad Ali, Linjuan Shi, Muhammad Aamir Khan, Ahmad Ali, Shuai Hu, Jinbo Shen","doi":"10.1111/ppl.70165","DOIUrl":"https://doi.org/10.1111/ppl.70165","url":null,"abstract":"Auxins are essential plant hormones that regulate growth, development, and responses to environmental stressors. Plants frequently encounter challenges such as pests, diseases, high temperatures, drought, and salinity, which necessitate adaptive mechanisms for survival. Auxins modulate stress-responsive signaling pathways by regulating gene expression and interacting with other phytohormones, thereby influencing physiological processes that maintain homeostasis under stress conditions. This review elucidates the molecular mechanisms through which auxins mediate plant responses to biotic and abiotic stresses. The findings indicate that auxins are pivotal in activating defense mechanisms and regulating stress signaling pathways. Differential expression of auxin-related genes has been observed in various crops under stress conditions, underscoring their role in enhancing resistance against pathogens and improving drought tolerance. Additionally, auxins influence root architecture and growth responses, facilitating adaptations such as trichome development for defense against herbivory. Moreover, the interplay between auxin signaling and other phytohormones is crucial for effective stress responses. Overall, auxins play a multifaceted role in enabling plants to cope with environmental stresses by regulating growth and activating defense mechanisms. Understanding these complex signaling pathways involving auxins can inform future research aimed at engineering resilient plant varieties capable of thriving in changing climates. Further studies are needed to clarify the specific functions of auxin in various stress contexts and to develop practical applications for crop improvement.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70165"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maize mutants in miR394-regulated genes show improved drought tolerance. mir394调控基因的玉米突变体表现出更高的耐旱性。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70155

Franco Miskevish, Anabella Lodeyro, María Agustina Ponso, Carlos Bouzo, Robert Meeley, Marja C Timmermans, Marcela Dotto

{"title":"Maize mutants in miR394-regulated genes show improved drought tolerance.","authors":"Franco Miskevish, Anabella Lodeyro, María Agustina Ponso, Carlos Bouzo, Robert Meeley, Marja C Timmermans, Marcela Dotto","doi":"10.1111/ppl.70155","DOIUrl":"https://doi.org/10.1111/ppl.70155","url":null,"abstract":"Water limitation represents one of the major threats to agricultural production, which often leads to drought stress and results in compromised growth, development and yield of crop species. Drought tolerance has been intensively studied in search of potential targets for molecular approaches to crop improvement. However, drought adaptive traits are complex, and our understanding of the physiological and genetic basis of drought tolerance is still incomplete. The miR394-LCR pathway is a conserved regulatory module shown to participate in several aspects of plant growth and development, including stress response. Here, we characterized the miR394 pathway in maize, which harbours two genetic loci producing an evolutionarily conserved mature zma-miR394 targeting two transcripts coding for F-Box proteins, named hereby ZmLCR1 and ZmLCR2. Arabidopsis plants overexpressing the zma-MIR394B gene showed high tolerance to drought conditions compared to control plants. Moreover, analysis of the growth and development of single and double maize mutant plants in ZmLCR genes indicate that these mutations do not affect plant fitness when they grow in normal watering conditions, but mutants showed better survival than wild-type plants under water deprivation conditions. This increased drought tolerance is based on more efficient intrinsic water use, changes in root architecture and increased epicuticular wax content under water-limiting conditions. Our results indicate that the miR394-regulated ZmLCR genes are involved in drought stress tolerance and are remarkable candidates for maize crop improvement.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70155"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MtMAP3Kδ6 Modulates the Growth and Development through Sugar Metabolism Regulation in Medicago truncatula. MtMAP3Kδ6通过糖代谢调控短叶苜蓿生长发育

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70208

Nan Ma, Haotian Li, Ting Liu, Hanwen Zhang, Jiangnan Yi, Cai Gao, Jiaxin Ma, Peizhi Yang, Tianming Hu, Yuman Cao

{"title":"MtMAP3Kδ6 Modulates the Growth and Development through Sugar Metabolism Regulation in Medicago truncatula.","authors":"Nan Ma, Haotian Li, Ting Liu, Hanwen Zhang, Jiangnan Yi, Cai Gao, Jiaxin Ma, Peizhi Yang, Tianming Hu, Yuman Cao","doi":"10.1111/ppl.70208","DOIUrl":"10.1111/ppl.70208","url":null,"abstract":"Plant growth and development are intricately regulated by molecular mechanisms, with the mitogen-activated protein kinase (MAPK) signaling cascade and its associated modules being pivotal. In this study, we identified and characterized a member of the MAPKKK family, MtMAP3Kδ6, from Medicago truncatula. This gene, classified within the B3 subgroup of the MAPKKK family, was expressed across various tissues during plant growth. The knockout mutant of MtMAP3Kδ6 displayed dwarfism, characterized by reduced branching and smaller leaf size, whereas overexpression of MtMAP3Kδ6 in Medicago truncatula led to the converse phenotypes. Transcriptome analysis and subsequent validation in leaves from different strains showed that the knockout mutants of MtMAP3Kδ6 had decreased levels of starch and sucrose, along with diminished cell wall invertase (INV; EC 3.2.1.26) activity, whereas overexpression resulted in the opposite effects. Collectively, our findings suggest that MtMAP3Kδ6 plays a role in Medicago truncatula growth and development by positively modulating sugar metabolism. This research lays a theoretical groundwork for future studies on the role of MAPKKK in sugar metabolism and its implications for plant growth and development.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70208"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergism of Bacillus subtilis and Azospirillum brasilense for enhanced N-use efficiency and maize growth: Evidence from ¹⁵N isotopic and physiological responses. 枯草芽孢杆菌和巴西氮螺旋菌对提高氮素利用效率和玉米生长的协同作用：来自15N同位素和生理反应的证据

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70205

Fernando Shintate Galindo, Cassio Carlette Thiengo, Paulo Humberto Pagliari, João Victor Silva Bernardes, Gustavo Duprat Dos Santos, Pedro Augusto Fedato Longato, Lucila de Sousa Vilela, Marcelo Carvalho Minhoto Teixeira Filho, José Lavres

{"title":"Synergism of Bacillus subtilis and Azospirillum brasilense for enhanced N-use efficiency and maize growth: Evidence from 15N isotopic and physiological responses.","authors":"Fernando Shintate Galindo, Cassio Carlette Thiengo, Paulo Humberto Pagliari, João Victor Silva Bernardes, Gustavo Duprat Dos Santos, Pedro Augusto Fedato Longato, Lucila de Sousa Vilela, Marcelo Carvalho Minhoto Teixeira Filho, José Lavres","doi":"10.1111/ppl.70205","DOIUrl":"https://doi.org/10.1111/ppl.70205","url":null,"abstract":"We explored the impact of inoculating maize (Zea mays L.) seeds with plant growth-promoting rhizobacteria (PGPR - Bacillus subtilis and Azospirillum brasilense), either individually or in combination, under different nitrogen input levels - control, low, average and high N levels (0, 30, 60, 90 and 120 mg L-1 equivalent to between 0 and 240 kg N ha-1) in a greenhouse setting. Leaf- and plant-level biometrics, nutritional, biochemical, and physiological evaluations were supplemented by isotopic methods (15N isotope dilution and natural abundance - δ15N‰) along with root scanning to investigate N acquisition and distribution. Dual inoculation not only enhanced the recovery of applied N but also bolstered nitrogenase activity, leading to increased biological N fixation (BNF) even at an average level of N (120 kg N ha-1). In instances where dual inoculated plants were grown at low (60 kg N ha-1) and average N levels, a cascade effect was observed, such as encompassing root growth stimulation, enhanced fertilizer and soil exploitation, and increased biomass production. Increasing N application to 240 kg N ha-1 reduced plant biomass by 19-47% compared to 120 kg N ha-1, depending on inoculation strategy. This led to downregulation of nitrogenase activity, diminished PGPR efficiency, and a significant decline in BNF. This study shows how a change in the rhizosphere microbial population can influence a myriad of responses inside the plants. Our research provides valuable insights for studies using 15N isotopic and dual inoculation techniques, offering strong potential for practical implementation.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70205"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0