Physiologia plantarum最新文献_第3页

The Mechanism of Micrografting With Salt-Tolerant Rootstock in Improving the Salt Tolerance of Scion in Vitis vinifera. 耐盐砧木微嫁接提高葡萄接穗耐盐性的机理研究。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70403

Ying Lai, Guojie Nai, Haokai Yan, Ping Sun, Jinyu Bao, Zhilong Li, Guiping Chen, Jingrong Zhang, Jianping Wang, Lei Ma, Sheng Li, Shaoying Ma

{"title":"The Mechanism of Micrografting With Salt-Tolerant Rootstock in Improving the Salt Tolerance of Scion in Vitis vinifera.","authors":"Ying Lai, Guojie Nai, Haokai Yan, Ping Sun, Jinyu Bao, Zhilong Li, Guiping Chen, Jingrong Zhang, Jianping Wang, Lei Ma, Sheng Li, Shaoying Ma","doi":"10.1111/ppl.70403","DOIUrl":"https://doi.org/10.1111/ppl.70403","url":null,"abstract":"Grapevine (Vitis vinifera L.) is highly sensitive to soil salinization, which severely restricts its cultivation in salt-affected areas. In this study, \"Pinot Noir\" (V. vinifera \"Pinot Noir\") was micro-grafted onto the salt-tolerant rootstock \"Kangzhen No. 3\" to explore the mechanisms by which rootstock-mediated micrografting enhances scion salt tolerance. Grafted seedlings, un-grafted scions, and rootstocks were irrigated with 200 mmol/L NaCl for 6 days. Physiological assessments and transcriptomic analysis revealed that grafted plants exhibited significantly improved salt tolerance compared to ungrafted \"Pinot Noir.\" Differentially expressed genes were mainly enriched in plant hormone signal transduction, MAPK signaling, and phenylpropanoid biosynthesis pathways. Two key genes, VvFLS and VvGSTU14, were selected for functional validation. Overexpression in grapevine calli enhanced antioxidant capacity and significantly improved salt tolerance. These findings demonstrate that micrografting with a salt-tolerant rootstock can enhance scion performance under saline stress by modulating key signaling and metabolic pathways, providing a theoretical foundation for grapevine improvement and sustainable production on saline soils.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70403"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144650158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological, Physiological, and Biochemical Impacts of Drought on Wheat-Pest-Pathogen Interactions. 干旱对小麦-病虫害相互作用的形态、生理和生化影响。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70364

Maroa Mohammed Al-Aisaee, Rethinasamy Velazhahan, Ahmad Nawaz, Muhammad Farooq

{"title":"Morphological, Physiological, and Biochemical Impacts of Drought on Wheat-Pest-Pathogen Interactions.","authors":"Maroa Mohammed Al-Aisaee, Rethinasamy Velazhahan, Ahmad Nawaz, Muhammad Farooq","doi":"10.1111/ppl.70364","DOIUrl":"https://doi.org/10.1111/ppl.70364","url":null,"abstract":"Plants often face a combination of abiotic and biotic stresses, such as drought, disease, and insect infestation. However, the interactions among these stressors remain poorly understood. This study investigates the effects of combined drought and biotic stress, particularly leaf rust (Puccinia triticina) and aphid (Rhopalosiphum maidis) infestations, on the wheat genotype TW1509. Two experiments were conducted under three water regimes: well-watered (WW; 80% WHC), moderate drought (MD; 60% WHC), and severe drought (SD; 40% WHC). MD intensified aphid and rust infestations, likely due to increased plant vulnerability. Aphid stress led to irregular stomatal patterns, reduced chlorophyll, and impaired photosynthesis. Under combined drought-aphid stress, significant increases were observed in stomatal density, electrolyte leakage, flavonoid content, and proline accumulation across all drought levels, along with reduced photosynthetic activity. Similarly, drought-rust stress elevated flavonoids, catalase activity, and proline content, though these were linked to decreased morphological traits. These findings highlight the complexity of wheat responses to concurrent stresses. The findings suggest that drought severity modulates plant susceptibility to pests and pathogens by influencing physiological resilience, nutrient dynamics, and defense responses. This study highlights the necessity of integrating multi-stress considerations into wheat management strategies to ensure sustainable productivity. A deeper understanding of these stress interactions is crucial for developing targeted interventions to mitigate their adverse effects on wheat.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70364"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-Photoperiod Interaction in Rice Phenology for Climate Adaptation: Insights Into Glycerate-Associated Metabolic Responses. 水稻物候中温度-光周期相互作用对气候适应的影响：对甘油相关代谢反应的见解。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70368

Hyeon-Seok Lee, Ju-Hee Kim, So-Hye Jo, Seo-Yeong Yang, Jae-Kyeong Baek, Yeong-Seo Song, Ji-Young Shon, Nam-Jin Chung

{"title":"Temperature-Photoperiod Interaction in Rice Phenology for Climate Adaptation: Insights Into Glycerate-Associated Metabolic Responses.","authors":"Hyeon-Seok Lee, Ju-Hee Kim, So-Hye Jo, Seo-Yeong Yang, Jae-Kyeong Baek, Yeong-Seo Song, Ji-Young Shon, Nam-Jin Chung","doi":"10.1111/ppl.70368","DOIUrl":"10.1111/ppl.70368","url":null,"abstract":"Rice heading date is tightly regulated by photoperiod and temperature, which are critical environmental cues for climate adaptation. While photoperiodic control of flowering has been well characterized, the molecular and metabolic mechanisms underlying temperature responses and their interaction with photoperiod remain unclear. In this study, we used two Oryza sativa ssp. japonica cultivars under controlled conditions to investigate the effects of temperature (22°C vs. 28°C) and photoperiod (12 vs. 14.5 h) during the photo-sensitive period. Integrative transcriptomic and metabolomic analyses identified key regulators of heading time, with particular focus on glycerate metabolism. Thermosensitivity increased threefold under short-day conditions, while photosensitivity was enhanced under high temperature. Glycerate, a pivotal intermediate in photorespiration and glycolysis, showed an inverse correlation with days to heading and accumulated more strongly in leaves under short-day and high-temperature conditions. Exogenous glycerate application (250-500 μM) accelerated heading by 4-5 days, supporting its functional role in floral induction. These findings highlight glycerate- and serine-associated metabolic pathways in regulating heading responses to environmental cues, providing new perspectives for optimizing heading time and enhancing climate resilience in rice production.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70368"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pepper JAZ Protein CaJAZ1-06 Negatively Regulates Drought Stress and Abscisic Acid Signaling. 辣椒JAZ蛋白CaJAZ1-06负调控干旱胁迫和脱落酸信号通路

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70390

Junyoung Cho, Chae Woo Lim, Dae Sung Kim, Sung Chul Lee

{"title":"Pepper JAZ Protein CaJAZ1-06 Negatively Regulates Drought Stress and Abscisic Acid Signaling.","authors":"Junyoung Cho, Chae Woo Lim, Dae Sung Kim, Sung Chul Lee","doi":"10.1111/ppl.70390","DOIUrl":"https://doi.org/10.1111/ppl.70390","url":null,"abstract":"JASMONATE ZIM-DOMAIN (JAZ) proteins are well-known key JA signaling repressors that play a crucial role in regulating various abiotic stresses, such as drought, salinity, and cold. However, their functional involvement in response to drought stress in pepper plants remains elusive. Here, we isolated a novel JAZ protein, namely, CaJAZ1-06 (Capsicum annuum JAZ1 from chromosome 06), from pepper plants and analyzed the expression of CaJAZ1-06 under drought stress conditions. Our results showed that its expression significantly increased compared with that of other pepper JAZ proteins, including its homologous gene CaJAZ1-03, a negative regulator of drought stress and ABA signaling. We also investigated the biological role of CaJAZ1-06 by silencing its expression in pepper plants through virus-induced gene silencing and by overexpressing it in Arabidopsis thaliana transgenic plants. In response to drought stress, CaJAZ1-06-silenced pepper plants showed enhanced tolerance, accompanied by a reduced transpirational water loss and increased expression of stress-responsive genes compared with the control plants. Conversely, the CaJAZ1-06 overexpressing Arabidopsis plants exhibited reduced drought tolerance, with increased transpirational water loss relative to the control plants. Furthermore, CaJAZ1-06 overexpression resulted in ABA insensitivity during seedling and adult stages. Therefore, our findings suggest that CaJAZ1-06 acts as a negative regulator of drought stress responses and ABA signaling.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70390"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular ATP Receptors P2Ks Play a Role in Regulating Local and Systemic ROS Accumulation of Arabidopsis thaliana Seedlings in Response to Local Salt Stress. 细胞外ATP受体P2Ks参与调控拟南芥幼苗局部和全身ROS积累对局部盐胁迫的响应

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70381

Yuejing Zhang, Xin Wang, Hetian Sang, Yuting Cai, Hailong Pang, Lingyun Jia, Wenliang Li, Hanqing Feng

{"title":"Extracellular ATP Receptors P2Ks Play a Role in Regulating Local and Systemic ROS Accumulation of Arabidopsis thaliana Seedlings in Response to Local Salt Stress.","authors":"Yuejing Zhang, Xin Wang, Hetian Sang, Yuting Cai, Hailong Pang, Lingyun Jia, Wenliang Li, Hanqing Feng","doi":"10.1111/ppl.70381","DOIUrl":"https://doi.org/10.1111/ppl.70381","url":null,"abstract":"In Arabidopsis (Arabidopsis thaliana), the lectin receptor kinases I.9 (P2K1) and I.5 (P2K2) are known as the receptors of extracellular ATP (eATP) and play important roles in the regulation of the responses of plants to environmental stresses. In the presented work, the possible involvement of these receptors of eATP in regulating local and systemic ROS (reactive oxygen species) accumulation to local salt stress was investigated. The roots or a single leaf of the seedlings were subjected to local salt stress, and the leaves that did not directly experience salt stress were regarded as the systemic organs. The results showed that local treatment with salt stress caused a local and systemic increase in eATP levels. Local application of exogenous ATP to the roots or a single leaf can evoke local and systemic ROS accumulation. Interestingly, local salt stress-induced local accumulation of ROS was weakened by P2K mutations, which also attenuated the systemic accumulation of ROS induced by local salt stress. In addition, local and systemic increases in H2O2 (hydrogen peroxide) content and CAT (catalase) activity by local NaCl stress were also reduced by the P2K mutations. These results suggest that the eATP receptors play an important role in regulating local and systemic ROS accumulation in response to local salt stress.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70381"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Omics Landscape to Reveal the Discrepancy in the Accumulation of Flavonoids in Artemisia argyi Across Multiple Growth Stages. 多组学研究揭示艾叶不同生长阶段黄酮类化合物积累的差异。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70377

Chunyan Miao, Mengzhi Li, Mingjie Li, Li Gu, Zhongyi Zhang, Li Gao, Xianzhang Huang, Zhanhu Cui

{"title":"Multi-Omics Landscape to Reveal the Discrepancy in the Accumulation of Flavonoids in Artemisia argyi Across Multiple Growth Stages.","authors":"Chunyan Miao, Mengzhi Li, Mingjie Li, Li Gu, Zhongyi Zhang, Li Gao, Xianzhang Huang, Zhanhu Cui","doi":"10.1111/ppl.70377","DOIUrl":"https://doi.org/10.1111/ppl.70377","url":null,"abstract":"Flavonoids are a major component of Artemisia argyi and play a crucial role in its pharmacological properties. However, the molecular mechanisms underlying flavonoid biosynthesis in A. argyi remain unclear. To address this, transcriptome and quantitative metabolome analyses were conducted across five developmental stages of A. argyi. In total, 85 flavonoid compounds were identified across these different stages. Differentially expressed candidate genes and metabolites involved in flavonoid biosynthesis were also identified. Differentially accumulated flavonoid metabolites (DFMs) were observed among the 10 comparison groups, with 29 DFMs identified from the five developmental stages of A. argyi leaves. The biosynthesis process identified 38 differentially expressed genes (DEGs) from seven gene families. Furthermore, 26 DEGs exhibited a significant correlation with the levels of seven active flavonoid metabolites, as revealed by weighted gene co-expression network analysis. These DEGs included eight HCT genes, six CHI genes, two CHS genes, three CCoAOMT genes, two F3'H genes, two C4H genes, two CYP98A genes, and one F3H gene. Based on preliminary analysis, HCT1 may be associated with accumulating hispidulin and jaceosidin. This study investigated the relationship between differential gene expression and flavonoid accumulation using an integrated transcriptomic and metabolomic approach, providing valuable insights into the mechanisms of flavonoid biosynthesis and quality formation in A. argyi.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70377"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Melatonin's Multifaceted Roles in Regulating Phytohormone Interactions During Plant Abiotic Stress Responses. 探索褪黑激素在植物非生物胁迫反应中调控植物激素相互作用的多方面作用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70354

Vinaykumar Rachappanavar

引用次数: 0

Balancing Vitamin B6 Homeostasis for Plant Immunity. 平衡维生素B6对植物免疫的体内平衡。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70415

Leeza Tariq, Yan Bi, Hui Wang, Mengmeng Guo, Fengming Song

{"title":"Balancing Vitamin B6 Homeostasis for Plant Immunity.","authors":"Leeza Tariq, Yan Bi, Hui Wang, Mengmeng Guo, Fengming Song","doi":"10.1111/ppl.70415","DOIUrl":"https://doi.org/10.1111/ppl.70415","url":null,"abstract":"Vitamin B6 (VB6), a metabolic cofactor, is essential for plants, animals, and microorganisms. VB6 has emerged as an important regulator of plant immunity, modulating various immune responses, including basal immunity, stomatal defence, and induced systemic resistance. However, the role of VB6 in systemic acquired resistance (SAR) and the active VB6 vitamers involved in modulating plant immunity remain unclear. A recent study reveals that Δ1-piperideine-6-carboxylic acid (P6C), a metabolite from lysine catabolism, sequesters VB6 vitamers, such as pyridoxal (PL) and pyridoxal 5'-phosphate (PLP), into inert complexes. This depletion of active VB6 vitamers disrupts VB6 homeostasis, impairing SAR, while exogenous application of VB6 restores resistance. Interestingly, the resemblance of this mechanism to pyridoxine-dependent epilepsy in humans suggests evolutionary conservation of these metabolic processes. This article explores the multifaceted roles of VB6 in different aspects of plant immunity. We emphasise the need for further research to decode the complex interplay between VB6 homeostasis and defence hormone pathways to deepen our understanding of immune signalling and develop new strategies to enhance stress resilience in plants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70415"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144650156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive Glucosinolate-Rich Extract Promotes Growth in Broccoli Seedlings by Modulating Energy Allocation. 富含硫代葡萄糖苷的生物活性提取物通过调节能量分配促进西兰花幼苗生长。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70391

Lorena Albaladejo-Marico, Micaela Carvajal, Lucia Yepes-Molina

{"title":"Bioactive Glucosinolate-Rich Extract Promotes Growth in Broccoli Seedlings by Modulating Energy Allocation.","authors":"Lorena Albaladejo-Marico, Micaela Carvajal, Lucia Yepes-Molina","doi":"10.1111/ppl.70391","DOIUrl":"10.1111/ppl.70391","url":null,"abstract":"Bioactive extracts derived from plants are emerging as the most innovative and promising alternative to traditional stimulants/chemicals in the agricultural market, given their high availability and rich content of bioactive compounds. Previously, the group synthesized and characterized a Brassicacea extract rich in secondary metabolites such as glucosinolates and phenols, which demonstrated a biostimulant effect in broccoli (Brassica oleracea L. var. italica) seedlings. Consequently, the main objective of this study was to investigate in detail the molecular mechanisms responsible for the stimulant capacity exhibited by the Brassicacea extract. For this aim, RNA sequencing was carried out to analyze gene expression in broccoli seedlings grown with the extract for 2 weeks, in combination with physiological measurements related to mineral composition, root transport, photosynthesis, and secondary metabolism. Treated seedlings exhibited an increase in macronutrients such as K, Ca, Mg, and S, along with a significant up-regulation of aquaporin genes and an enhancement in relative water content (RWC), indicating a clear improvement in mineral and water homeostasis. Additionally, root structure was enhanced, correlating with the overexpression of genes associated with suberin synthesis. Moreover, a general activation of genes involved in energy production, including those of the Krebs cycle, was observed. The results revealed that the extract plays a key role in modulating plant metabolism by shifting resources away from secondary metabolism and redirecting them toward primary metabolism, ultimately promoting a higher growth rate.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70391"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Functional Analysis of the Fragrance Terpene Synthase Gene PpTPS5 in Pyrethrum parthenium. 除虫菊香精萜合成酶基因PpTPS5的鉴定与功能分析。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-07-01 DOI: 10.1111/ppl.70333

Fang Wang, Daihao Li, Sixian Zeng, Xueqian Gao, Guilan Wu, Di Huang, Zihan Cao, Qin Wang, Rangcai Yu, Yanping Fan

{"title":"Identification and Functional Analysis of the Fragrance Terpene Synthase Gene PpTPS5 in Pyrethrum parthenium.","authors":"Fang Wang, Daihao Li, Sixian Zeng, Xueqian Gao, Guilan Wu, Di Huang, Zihan Cao, Qin Wang, Rangcai Yu, Yanping Fan","doi":"10.1111/ppl.70333","DOIUrl":"https://doi.org/10.1111/ppl.70333","url":null,"abstract":"Pyrethrum parthenium, a perennial herb from the genus Pyrethrum within the Asteraceae family, is known for its potent fragrance and significant economic value. Nevertheless, the mechanisms involved in the synthesis and regulation of its floral fragrance compounds remain unclear. This study used gas chromatography-mass spectrometry (GC-MS) to analyze the volatile compounds in five parts of P. parthenium: tubular flowers, ligulate flowers, sepals, receptacles, and leaves. The results showed that the volatile aromatic components include terpenes such as camphor, α-pinene, camphene, d-limonene, β-caryophyllene, and β-farnesene, with camphor being the main volatile compound. Based on the transcriptome data of tubular flowers of P. parthenium, a terpene synthase gene, PpTPS5, was identified. The results of both in vitro and in vivo enzymatic assays demonstrated that PpTPS5 functions as a bifunctional terpene synthase gene. The qRT-PCR results showed that the gene expression pattern of PpTPS5 is correlated with the release pattern of the corresponding terpene aromatic compounds in P. parthenium. Subcellular localization and inhibitor experiments indicated that PpTPS5 functions in the cytosol. Additionally, dual-luciferase and electrophoretic mobility shift assay (EMSA) assays revealed that PpMYB9 can bind to the promoter of PpTPS5 to regulate the biosynthesis of terpenoids. In conclusion, the results of this study provide a theoretical basis for further exploration of the transcriptional regulation of terpene floral fragrance compounds in P. parthenium.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70333"},"PeriodicalIF":5.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0