Physiologia plantarum最新文献_第3页

STADIA: A Spatio-Temporal Deep-Learning Framework for Cross-Stage Integration and Batch Correction of Plant Developmental Spatial Transcriptomics. STADIA：植物发育空间转录组学跨阶段整合和批量校正的时空深度学习框架。

IF 4 2区生物学

Physiologia plantarum Pub Date : 2026-09-01 DOI: 10.1111/ppl.71143

Dezhi Zhi, Xuemei Guan, Liuyan Wang, Wenhui Chen

{"title":"STADIA: A Spatio-Temporal Deep-Learning Framework for Cross-Stage Integration and Batch Correction of Plant Developmental Spatial Transcriptomics.","authors":"Dezhi Zhi, Xuemei Guan, Liuyan Wang, Wenhui Chen","doi":"10.1111/ppl.71143","DOIUrl":"https://doi.org/10.1111/ppl.71143","url":null,"abstract":"Spatial transcriptomics enables the spatial architecture and transcriptional dynamics of plant organ development to be resolved in situ. However, integrating plant spatial transcriptomic data across developmental stages is challenging because genuine developmental variation can be confounded with technical variation arising from section source, capture region and sequencing batch, making it difficult to attenuate technical effects without compromising developmental signals. To address this challenge, we developed STADIA, a spatio-temporal deep-learning framework for plant developmental spatial transcriptomics. STADIA combines a graph-attention autoencoder with structural-context-conditioned batch-predictability suppression, stage-order constraints, PCGrad-based gradient coordination and hierarchical unbalanced optimal transport to learn an integrated latent representation that attenuates batch-associated variation while preserving spatial organization and developmental continuity and accommodating non-equivalent tissue transitions. In a semi-synthetic benchmark, STADIA maintained balanced performance across batch mixing, anatomical-domain preservation and developmental-information preservation under strong batch effects and high sparsity. In the same-stage replicate sections from the soybean early-maturation stage (EMS), STADIA increased local between-section mixing while retaining major anatomical compartments. In a poplar stem developmental series spanning the shoot apex to internode 7, the integrated representation showed increased cross-stage continuity while retaining stage-specific organization and spatially coherent tissue compartments, and supported exploratory identification of cambium-associated trajectory candidates. Together, these results support STADIA as a framework for cross-stage integration of plant spatial transcriptomic data and for downstream analysis of spatial domains and developmentally ordered transcriptional patterns.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71143"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149183879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Causal Validation of Mobile RNA and Extracellular Vesicle Signals in Plants: Experimental Frameworks for Sequential Stress Acclimation. 植物中移动RNA和细胞外囊泡信号的因果验证：序列胁迫驯化的实验框架。

IF 4 2区生物学

Physiologia plantarum Pub Date : 2026-09-01 DOI: 10.1111/ppl.71123

Wajid Zaman, Adnan Amin

{"title":"Causal Validation of Mobile RNA and Extracellular Vesicle Signals in Plants: Experimental Frameworks for Sequential Stress Acclimation.","authors":"Wajid Zaman, Adnan Amin","doi":"10.1111/ppl.71123","DOIUrl":"https://doi.org/10.1111/ppl.71123","url":null,"abstract":"Long-distance molecular transport contributes to coordination between locally stressed tissues and distal organs, but assigning causal systemic-signaling functions to mobile RNAs and extracellular vesicle (EV)-associated molecules remains experimentally challenging. This review critically evaluates the evidence required to distinguish molecular association, source assignment, physical movement, recipient activity, persistence, and causal function in sequential-stress systems. We assess source-resolved RNA tracking, graft-based mobility assays, sequence-assignment and contamination errors, molecular integrity, route validation, EV source-fluid quality, particle identity and heterogeneity, cargo topology, vesicular versus non-vesicular extracellular RNA, recipient uptake, and functional delivery. Recent studies on mobile-mRNA assignment, stress-responsive graft-transmissible transcripts, plant EV proteomes and markers, EV isolation and characterization, and extracellular small-RNA carrier states are integrated to identify which inferences are experimentally supported and which remain provisional. Sequential-stress designs are considered separately because persistence must be distinguished from residual injury, prolonged acute responses, generalized sensitization, and stress-order effects. The objective of this review is to establish reproducible experimental and analytical criteria for assigning systemic-signaling functions to RNA and EV-associated carriers. We therefore propose an evidence hierarchy progressing from association and source assignment to movement, recipient activity, persistence, necessity, sufficiency, and rescue. This framework defines the minimum evidence needed to advance from molecular detection to causal assignment in studies of systemic plant acclimation.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71123"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149002433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CaCl₂ Priming Boosts Salinity-Alkalinity Tolerance in Germinating Soybean by Reducing DNA Oxidative Damage and Enhancing Ca²⁺ -ROS Signaling Crosstalk. CaCl2通过减少DNA氧化损伤和增强Ca2+ -ROS信号串扰提高发芽大豆耐盐碱性。

IF 4 2区生物学

Physiologia plantarum Pub Date : 2026-09-01 DOI: 10.1111/ppl.71055

Ran Sun, Qiang Zhao, Weiyu Wang, Yuan Yao, Hanjie Wang, Lijuan Li, Ningxin Fan, Hongxue Zhang, Jidao Du, Yanli Du

{"title":"CaCl2 Priming Boosts Salinity-Alkalinity Tolerance in Germinating Soybean by Reducing DNA Oxidative Damage and Enhancing Ca2+ -ROS Signaling Crosstalk.","authors":"Ran Sun, Qiang Zhao, Weiyu Wang, Yuan Yao, Hanjie Wang, Lijuan Li, Ningxin Fan, Hongxue Zhang, Jidao Du, Yanli Du","doi":"10.1111/ppl.71055","DOIUrl":"10.1111/ppl.71055","url":null,"abstract":"Soybean (Glycine max) seed germination is highly sensitive to saline-alkaline stress. Seed priming represents an effective strategy to mitigate its detrimental effects. However, the optimal priming conditions (agent, concentration, duration) and the underlying molecular mechanisms remain poorly understood. This study investigated the effects of priming with distilled water (Control), calcium chloride (CaCl2), melatonin (MT), and proline (Pro) under saline-alkaline stress on soybean seed germination and the molecular basis of enhanced tolerance. Evaluation of ten germination-related parameters revealed that priming with 100 mM CaCl2 for 12 h significantly enhanced the germination rate. Physiological analyses demonstrated that CaCl2 priming effectively reduced reactive oxygen species (ROS) accumulation by increasing the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), while decreasing malondialdehyde (MDA) content. Furthermore, CaCl2 priming activated the Ca2+ signaling pathway by increasing radicle Ca2+ content and upregulating the expression levels of Ca2+ signaling-related genes (e.g., GmCAM7, GmCNGC2, GmCNGC19, GmMPK2, and GmMKK2). Additionally, CaCl2 priming significantly enhanced DNA damage repair capacity of soybean cultivars with differing saline-alkaline tolerance. This was manifested by reduced DNA oxidative damage and decreased random amplified polymorphic DNA (RAPD) polymorphism, thereby enhancing genomic stability and alleviating cell cycle arrest. These findings deepen our understanding of the complex regulatory role of calcium signaling in plant abiotic stress responses and provide important novel theoretical insights for improving crop resilience.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71055"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13559200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148920026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of the Simultaneous Loss of the Plastid Envelope-Localized Homologous Pair Ycf10 and DLDG1 in Plant Growth and Photosynthetic Performance. 质体包膜定位同源对Ycf10和DLDG1同时缺失对植物生长和光合性能的影响

IF 4 2区生物学

Physiologia plantarum Pub Date : 2026-09-01 DOI: 10.1111/ppl.71114

Elham Esmailpourmoghadam, Mai Duy Luu Trinh, Ryoichi Sato, Masaru Kono, Issei Nakazato, Shin-Ichi Arimura, Michael Palmgren, Shinji Masuda

{"title":"Impact of the Simultaneous Loss of the Plastid Envelope-Localized Homologous Pair Ycf10 and DLDG1 in Plant Growth and Photosynthetic Performance.","authors":"Elham Esmailpourmoghadam, Mai Duy Luu Trinh, Ryoichi Sato, Masaru Kono, Issei Nakazato, Shin-Ichi Arimura, Michael Palmgren, Shinji Masuda","doi":"10.1111/ppl.71114","DOIUrl":"10.1111/ppl.71114","url":null,"abstract":"Ycf10 and DLDG1 are homologous proteins embedded in the chloroplast envelope membranes (EM) and encoded in the plastidial and nuclear genomes, respectively. We previously characterized Arabidopsis dldg1 and tobacco ycf10 mutants, and showed that these proteins mediate proton transport across the EM, thereby influencing ATP synthesis and the non-photochemical quenching (NPQ) induction. However, the consequences of the simultaneous loss of Ycf10/DLDG1 homologs have remained unclear. Here, we generated and analyzed Arabidopsis ycf10 and ycf10-dldg1 double mutants. These mutants exhibited a pale-green phenotype under continuous light conditions, which was complemented by the exogenous addition of NaCl, as previously observed in mutants lacking the plastid EM-localized K+/H+ antiporters KEA1 and KEA2. Both single mutants (ycf10 and dldg1), as well as the ycf10-dldg1 double mutant, showed enhanced NPQ induction compared with wild-type. Furthermore, the ycf10-dldg1 double mutant showed stronger NPQ induction than either single mutant upon transition from dark to light, suggesting that Ycf10 and DLDG1 function independently while playing redundant roles. These mutants also showed reduced stomatal conductance and sugar accumulation compared with the wild-type. Together, these findings indicate that EM-localized DLDG1 and Ycf10 act in concert to maintain chloroplast proton/ion homeostasis, contributing to chloroplast pH homeostasis for sustaining plant growth and efficient photosynthesis.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71114"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13583143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148983317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Phyllosphere Microbial Regulation of Nitrogen Use in Rice. 水稻层际微生物调控氮素利用的机制

IF 4 2区生物学

Physiologia plantarum Pub Date : 2026-09-01 DOI: 10.1111/ppl.71138

Yu Yan, Zhiqian Yuan, Xiangyu Lin, Nan Zhang, Xinping Lv, Wenjiang Jing, Jianhua Zhang, Hao Zhang

{"title":"Mechanisms of Phyllosphere Microbial Regulation of Nitrogen Use in Rice.","authors":"Yu Yan, Zhiqian Yuan, Xiangyu Lin, Nan Zhang, Xinping Lv, Wenjiang Jing, Jianhua Zhang, Hao Zhang","doi":"10.1111/ppl.71138","DOIUrl":"https://doi.org/10.1111/ppl.71138","url":null,"abstract":"Nitrogen (N) fertilizers are key drivers of high yields in rice (Oryza sativa L.), yet increasing N inputs often deliver diminishing improvements in N use efficiency (NUE) and can exacerbate N losses via volatilization, leaching, and runoffs, with associated environmental impacts. The rice phyllosphere microbiome, which consists of microbes inhabiting leaf surfaces and internal tissues, has the potential to influence foliar nutrient turnover, N metabolism, and stress responses and may therefore influence internal NUE (IEN) and, under reduced fertilizer-N input, potentially affect agronomic efficiency of applied N (AEN). Here, we summarize the major steps of rice N metabolism (uptake, assimilation, transport, redistribution, and remobilization) and discuss microbial pathways that could modulate N use, including foliar N transformations, microbially mediated N inputs, and indirect effects through hormonal regulation, stress buffering, and microbe-microbe interactions. Key knowledge gaps remain in quantification of underlying mechanistic processes, field robustness across seasons and sites, and genotype/microbiome matching under reduced-N management.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71138"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149113515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Whole-Genome Sequence Resource for the Medicinal Rice Landrace Navara. 药用稻地方品种纳瓦拉全基因组序列资源。

IF 4 2区生物学

Physiologia plantarum Pub Date : 2026-09-01 DOI: 10.1111/ppl.71148

Praveena Murugan, Saumya Dwivedi, Nikitha Jayachandran, Manickavelu Alagu

{"title":"A Whole-Genome Sequence Resource for the Medicinal Rice Landrace Navara.","authors":"Praveena Murugan, Saumya Dwivedi, Nikitha Jayachandran, Manickavelu Alagu","doi":"10.1111/ppl.71148","DOIUrl":"https://doi.org/10.1111/ppl.71148","url":null,"abstract":"Navara (Oryza sativa L.) is a traditional pigmented rice landrace from Kerala, India, recognized for its nutritional and medicinal importance. However, its genome-wide sequence variation and population-genetic context remain insufficiently characterized. Here, we present a whole-genome sequence resource for a single Navara accession, with particular emphasis on sequence variation in genes associated with anthocyanin and flavonoid biosynthesis. Paired-end whole-genome sequencing generated 58.7 million reads, providing approximately 30× genome coverage. Reference-guided analysis using the well-annotated IRGSP-1.0 genome identified 4,118,622 high-confidence variants, comprising 3,513,668 SNPs and 604,954 INDELs. Among the 19 selected anthocyanin- and flavonoid-related genes, 561 SNPs were identified and characterized according to their genomic context and predicted consequences. Comparison with the Rice3K dataset identified 131 variants in these genes that were not observed in the comparison dataset. Genome-wide principal component analysis placed the sequenced Navara accession closest to the Aus/boro genetic group, while identity-by-state analysis identified Karutha Seenati as the closest accession among those compared using the selected loci. Several variant-containing regions overlapped previously reported rice QTL intervals, providing genomic context for further investigation without implying genetic association or causality. De novo assembly of reference-unmapped reads generated approximately 9.29 Mb of additional sequence, including predicted genes and repetitive elements, while comparison with the PanOryza pangenome indicated that most predicted genes were represented within the broader rice gene pool. Collectively, these datasets provide a reusable genomic resource for comparative genomics, genetic-diversity analysis, marker development, conservation of traditional rice germplasm, and future functional investigation of pigmentation, nutritional, and agronomic traits.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71148"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149137671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sodium Hydrosulfide Delays Postharvest Softening of Sand Pear Fruit in Association With Altered Ethylene Production and Cell Wall Metabolism. 氢硫化钠延缓沙梨果实采后软化与乙烯产量和细胞壁代谢的改变有关。

IF 4 2区生物学

Physiologia plantarum Pub Date : 2026-09-01 DOI: 10.1111/ppl.71152

Jie Gao, Hai-Ying Ren, Peng-Yuan Wang, Chun-Lei Wang, Jing Zhang

{"title":"Sodium Hydrosulfide Delays Postharvest Softening of Sand Pear Fruit in Association With Altered Ethylene Production and Cell Wall Metabolism.","authors":"Jie Gao, Hai-Ying Ren, Peng-Yuan Wang, Chun-Lei Wang, Jing Zhang","doi":"10.1111/ppl.71152","DOIUrl":"https://doi.org/10.1111/ppl.71152","url":null,"abstract":"Sodium hydrosulfide (NaHS), a hydrogen sulfide donor, has been reported to regulate postharvest senescence and quality maintenance in horticultural crops. However, its role in sand pear softening and the underlying physiological basis remain unclear. Mature fruits of the climacteric sand pear cultivar \"Sucui No. 1\" were treated with NaHS and stored at 20°C to evaluate its effects on postharvest quality, ethylene production, cell wall metabolism, and softening-related gene expression. Treatment with 1 mmol L-1 NaHS delayed firmness loss and reduced ethylene production, with limited effects on fruit color, weight loss, and total soluble solids during storage. NaHS-treated fruit retained significantly higher firmness than control fruit from Day 15 onward, with the largest difference between treatments being 3.25 N on Day 15. NaHS-treated fruit retained higher levels of cellulose, hemicellulose, covalently soluble pectin, and ionically soluble pectin, and accumulated less water-soluble pectin, indicating delayed cell wall polysaccharide disassembly. Consistently, NaHS suppressed the activities of several cell wall-modifying enzymes, including cellulase, β-glucosidase, xyloglucan endotransglucosylase/hydrolase, polygalacturonase, and pectin methylesterase. RNA sequencing and qRT-PCR analyzes showed that NaHS modulated the expression of genes associated with cell wall remodeling, ethylene biosynthesis, sulfur-related metabolism, and transcriptional regulation, particularly during the early-to-middle storage stage. Transient silencing assays further indicated that the XTH gene (gene11051) was associated with cellulose-hemicellulose remodeling, whereas the two PME genes (gene26382 and gene8920) were associated with pectin solubilization. These results suggest that the delay in postharvest softening following NaHS treatment was associated with coordinated changes in ethylene production and cell wall disassembly.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71152"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149183831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strigolactones: From Fundamental Biology to Applications in Tree Breeding. 独角金内酯：从基础生物学到树木育种应用。

IF 4 2区生物学

Physiologia plantarum Pub Date : 2026-09-01 DOI: 10.1111/ppl.71064

Zhongzheng Ma, Wanxin Li, Qi Guo, Dativa Gosbert Tibesigwa, Qingfeng Meng, Fude Wang

{"title":"Strigolactones: From Fundamental Biology to Applications in Tree Breeding.","authors":"Zhongzheng Ma, Wanxin Li, Qi Guo, Dativa Gosbert Tibesigwa, Qingfeng Meng, Fude Wang","doi":"10.1111/ppl.71064","DOIUrl":"10.1111/ppl.71064","url":null,"abstract":"Strigolactones (SLs) are small carotenoid-derived signaling molecules that serve as both rhizosphere chemical cues and well-recognized endogenous plant hormones. They are involved in shoot branching inhibition, root system remodeling, mycorrhizal symbiosis, and diverse stress responses in plants. This paper systematically reviews the biosynthesis and metabolism of SLs, the perception and signaling mechanisms, their translocation and long-distance transport in planta, as well as how SLs modulate key traits of forest trees via crosstalk with other hormones, including auxin, cytokinin, abscisic acid, etc. Based on current technical platforms such as tissue resolved liquid chromatography tandem mass spectrometry (LC-MS/MS), isotope labeling, tissue specific clustered regularly interspaced short palindromic repeats (CRISPR) and inducible expression systems, grafting assays, and semifield long-term monitoring, this study proposes a research and breeding roadmap progressing from molecular validation to medium- and short-term characterization in model trees and finally to multisite long-term ecological assessment. It also provides recommendations covering risks, benefits, and regulations concerning the use of chemical analogs, gene editing, and grafting strategies in forest cultivation. This review presents a multiscale theoretical framework and testable pathways for translating SLs research from model plants to forestry applications.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71064"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13504695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148812775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaffolder Herbivory and Jasmonate Signalling Are Associated With Changes in Lipid-Derived Volatile Profiles in Rice. 水稻叶柄、草食和茉莉酸信号传导与脂质挥发谱的变化有关。

IF 4 2区生物学

Physiologia plantarum Pub Date : 2026-09-01 DOI: 10.1111/ppl.71129

Ramasamy Kanagaraj Murali-Baskaran, Yogesh Yele, Pankaj Sharma, Pramod Kumar Rai

{"title":"Leaffolder Herbivory and Jasmonate Signalling Are Associated With Changes in Lipid-Derived Volatile Profiles in Rice.","authors":"Ramasamy Kanagaraj Murali-Baskaran, Yogesh Yele, Pankaj Sharma, Pramod Kumar Rai","doi":"10.1111/ppl.71129","DOIUrl":"https://doi.org/10.1111/ppl.71129","url":null,"abstract":"Plants respond to insect herbivory by activating defence signalling pathways that regulate metabolism and volatile emissions. In rice, jasmonate signalling plays a central role in the production of herbivore-induced plant volatiles (HIPVs), yet the contribution of lipid-derived semi-volatile compounds to parasitoid-mediated tritrophic interactions remains poorly understood. Here, we studied the effects of rice leaffolder (Cnaphalocrocis medinalis) herbivory, exogenous jasmonic acid (JA) treatment and mechanical damage on volatile profiles in rice (Oryza sativa L.) and evaluated the behavioural responses of the egg parasitoid, Trichogramma chilonis. Volatile organic compounds were collected using a dynamic volatile trapping system and analysed by GC-MS. A total of 31 putatively identified compounds belonging to hydrocarbons, aldehydes, alcohols, esters and aromatic compounds were detected, with clear treatment-specific differences in composition and abundance. Herbivore infestation and JA treatment altered the relative abundance of several lipid-derived compounds, whereas the combined JA + herbivory treatment produced the most complex volatile blend, indicating interactive effects of hormonal signalling and herbivore-associated cues. Multivariate analyses further revealed distinct volatile signatures among treatments, demonstrating that herbivory, jasmonate signalling and mechanical damage differentially influence rice volatile composition. Behavioural bioassays showed that several lipid-derived compounds enhanced parasitisation by T. chilonis, with the long-chain aldehydes tetradecanal and hexadecanal eliciting particularly strong responses. Collectively, these findings suggest that jasmonate-associated changes in lipid-derived semi-volatile compounds may contribute to parasitoid recruitment and provide new insights into the potential role of medium- and long-chain lipid-derived volatiles in rice-mediated tritrophic interactions.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71129"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149040270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydraulic, Gas-Exchange and Leaf-Trait Variation Across Hydrologically Contrasting Sites and Tree Sizes in Populus euphratica. 胡杨水文对比立地和乔木的水力、气体交换和叶性状变化。

IF 4 2区生物学

Physiologia plantarum Pub Date : 2026-09-01 DOI: 10.1111/ppl.71150

Shubin Zhang, Xiaodong Yang, Zhoukang Li, Wusong Li, Guanghui Lv

{"title":"Hydraulic, Gas-Exchange and Leaf-Trait Variation Across Hydrologically Contrasting Sites and Tree Sizes in Populus euphratica.","authors":"Shubin Zhang, Xiaodong Yang, Zhoukang Li, Wusong Li, Guanghui Lv","doi":"10.1111/ppl.71150","DOIUrl":"https://doi.org/10.1111/ppl.71150","url":null,"abstract":"Plant water status, hydraulic transport and carbon exchange can respond differently across natural hydrologic contrasts. We measured hydraulic, gas-exchange, carbon, morphological and anatomical traits in 33 Populus euphratica trees distributed across four fixed sites (D1-D4) and three tree-size classes. Site and size associations were evaluated with additive models, heteroskedasticity-consistent inference, false-discovery-rate control and a balanced D1-D3 sensitivity analysis. Midday leaf water potential became progressively more negative from D1 to D4, whereas branch xylem hydraulic conductivity was higher at D3-D4. The correlated instantaneous responses of assimilation (A), stomatal conductance (gs), and transpiration (E) peaked at D3 and declined at D4. Within the balanced D1-D3 subset, site effects for midday water potential, hydraulic conductivity and the A-gs-E response group remained supported. Larger trees had lower specific leaf area, soluble sugar and total non-structural carbohydrates, but higher leaf water content and palisade tissue thickness; starch showed a consistent site association. After adjustment for site and size, no anatomy-function association based on total palisade tissue thickness (PT) or epidermis thickness (Epi) retained false-discovery-rate support. Hydrologic context and tree size were associated with partly distinct functional dimensions, indicating multidimensional and context-dependent intraspecific variation in P. euphratica.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71150"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149168292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0