Physiologia plantarum最新文献_第3页

Nitrogen Fertilizer Exerts Little Impact on Highbush Blueberry Floral Chemistry, Pollinator Visitation, Yield, and Fruit Quality. 氮肥对高丛蓝莓花化学、传粉者访视、产量和果实品质影响不大。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70526

Sagar Jadhav, Lisa W DeVetter, Gabriel T LaHue, Stephanie L Spencer, Angela Prudencio, Camila Caldana, Alisdair R Fernie, Monica Borghi

{"title":"Nitrogen Fertilizer Exerts Little Impact on Highbush Blueberry Floral Chemistry, Pollinator Visitation, Yield, and Fruit Quality.","authors":"Sagar Jadhav, Lisa W DeVetter, Gabriel T LaHue, Stephanie L Spencer, Angela Prudencio, Camila Caldana, Alisdair R Fernie, Monica Borghi","doi":"10.1111/ppl.70526","DOIUrl":"https://doi.org/10.1111/ppl.70526","url":null,"abstract":"Plants allocate chemical resources to nectar and pollen rewards to entice pollinators to flowers and encourage pollination success. However, plant mineral nutrients, when deficient or applied in excess using fertilizers, could affect the chemistry of nectar and pollen and, subsequently, pollinator preferences for the flowers; therefore, cross-pollination, yield, and quality of fruits. In this study, we analyzed the chemical composition of nectar and pollen and the rates of pollinator visitation in commercial fields of northern highbush blueberry (Vaccinium corymbosum L.) cultivar Duke supplemented with nitrogen fertilizer at three progressive rates. We further integrated these studies with measurements of transcript abundance of nitrogen and proline transporters, and affinity scores of transporter proteins for nitrate. Our analysis revealed moderate variation of proline, dehydroascorbate, and succinate concentration in nectar but no changes in the pollen chemistry and honey bee visitation or the yield and quality of berries. Our findings suggest that applying low rates of nitrogen fertilizer (33-55 kg N ha-1) can sustain the chemical quality of nectar and pollen, attract enough honey bee pollinators to support pollinator services, and ultimately maintain blueberry yield and quality.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70526"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-Wide Identification and Characterization of Circular RNAs Involved in High Night Temperature Stress at the Filling Stage of Rice. 水稻灌浆期高温胁迫相关环状rna的全基因组鉴定与特征分析。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70537

Jiangmin Fan, Hongyu Zhang, Xiaoya Zhou, Luyao Gu, Youmin Yao, Yan Shi, Chang Liu, Yuewu Li, Yuxiang He, Jianglin Liao, Yingjin Huang, Zhaohai Wang

{"title":"Genome-Wide Identification and Characterization of Circular RNAs Involved in High Night Temperature Stress at the Filling Stage of Rice.","authors":"Jiangmin Fan, Hongyu Zhang, Xiaoya Zhou, Luyao Gu, Youmin Yao, Yan Shi, Chang Liu, Yuewu Li, Yuxiang He, Jianglin Liao, Yingjin Huang, Zhaohai Wang","doi":"10.1111/ppl.70537","DOIUrl":"https://doi.org/10.1111/ppl.70537","url":null,"abstract":"High night temperatures seriously threaten the normal growth of rice, especially during the filling stage. Studies have shown that circRNAs play crucial roles in regulating plant stress response. Here, 3670 circRNAs were identified from a rice heat-tolerant line and a heat-sensitive line under high night temperature stress at the filling stage. 546 circRNAs showed conservation among the plant species, and 396 circRNAs owned potential protein translation abilities. The expression of the circRNAs was verified using PCR and sequencing. Further, 103 and 118 circRNAs were separately found to be differentially expressed in these two rice lines. The regulatory relationship of circRNA-parental gene pairs and the circRNA-miRNA-mRNA ceRNA network was constructed to reveal the function of these circRNAs. Functional enrichment analysis indicated that these circRNAs are involved in heat stress-related biological processes and metabolic pathways. Finally, 28 circRNAs showed significant relative expression changes between the heat-tolerant line and heat-sensitive line, being possibly involved in the differential heat sensitivity phenotype. Sixteen circRNA-parental gene pairs and nine circRNA-20 miRNA-242 mRNA ceRNA regulatory networks were additionally obtained and partially validated by qRT-PCR. Functional analysis of these regulated genes indicated that high night temperature-responsive circRNAs were involved in various heat-related biological processes, such as transcription regulation, nutrient metabolism, stress response, hormone regulation, redox processes, chloroplast regulation, and protein folding, which are discussed in detail here. This study provides new insights into the potential role of circRNAs responding to high night temperature stress at the filling stage of rice and provides new ideas for rice heat-tolerance breeding.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70537"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SlSPL15: A Negative Regulator Targeted by SlmiR156a Participates in Regulating the Light-Induced Anthocyanin Biosynthesis of Aft Tomato Fruits. SlmiR156a靶向的SlSPL15负调控因子参与光诱导番茄后果花青素生物合成

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70471

Chuyao Xu, Siyue Qi, Fuchang Guo, Hui Wang, Ji Li, Jiazhen Li, Weilin Wu, Bo Zhou

{"title":"SlSPL15: A Negative Regulator Targeted by SlmiR156a Participates in Regulating the Light-Induced Anthocyanin Biosynthesis of Aft Tomato Fruits.","authors":"Chuyao Xu, Siyue Qi, Fuchang Guo, Hui Wang, Ji Li, Jiazhen Li, Weilin Wu, Bo Zhou","doi":"10.1111/ppl.70471","DOIUrl":"https://doi.org/10.1111/ppl.70471","url":null,"abstract":"Anthocyanins are crucial compounds known for their antioxidant and health benefits. The Aft tomato (Solanum lycopersicum) line LA1996 can generate anthocyanins in fruits upon light exposure. Despite the identification of various regulatory genes, such as microRNAs and transcription factors involved in anthocyanin biosynthesis across different plant species, the function of the miR156/SPL module in Aft tomato fruit pigmentation is not well understood. In this research, 17 SlSPL family genes of Aft tomato were classified into six clades. SlSPL15 (Solyc10T002263.1) was grouped in Clade V, with AtSPL9, which is known to be involved in anthocyanin biosynthesis in Arabidopsis. Moreover, an inverse relationship between SlSPL15 and miR156a expression in mature green (MG) stage fruits was shown by quantitative RT-PCR analysis. Transient expression analysis of luciferase confirmed that SlSPL15 transcripts are degraded by SlmiR156a. Furthermore, overexpression of SlSPL15 in Aft tomato reduced the anthocyanin accumulation in MG fruits. In SlSPL15 overexpressed tomato plants, the transcription level of SlSPL15 was elevated compared to that in wild-type fruits, whereas the expression of SlPAL, SlCHS1, SlDFR, SlF3H, SlF3'5'H, and SlANS was reduced. Additionally, the expression of light-responsive regulatory genes SlHY5, SlAN2-like, and SlMYB12 in the anthocyanin biosynthetic pathway was also reduced in light-exposed fruits of 35S:SlSPL15 plants. Subcellular localization analysis verified that SlSPL15 is localized in the nucleus, while yeast two-hybrid assays demonstrated its interaction with SlAN2-like, a part of the MBW complex that participates in regulating anthocyanin biosynthesis in Aft tomato fruits. The findings expand our comprehension of the roles of SlSPL15, targeted by SlmiR156a, in regulating light-induced anthocyanin accumulation in tomatoes.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70471"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12397961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cysteine Alleviates the Toxicity of Cadmium Telluride Quantum Dots (CdTe QDs) by Modulating the Antioxidant System and Root Metabolic Pathways in Rice. 半胱氨酸通过调控水稻抗氧化系统和根系代谢途径减轻碲化镉量子点（CdTe QDs）的毒性。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70493

Qiu-Yun Shi, Teng Li, Hao Xiang, Yang Wu, Shu Yuan, Ming Yuan, Shozeb Haider, Yang-Er Chen

{"title":"Cysteine Alleviates the Toxicity of Cadmium Telluride Quantum Dots (CdTe QDs) by Modulating the Antioxidant System and Root Metabolic Pathways in Rice.","authors":"Qiu-Yun Shi, Teng Li, Hao Xiang, Yang Wu, Shu Yuan, Ming Yuan, Shozeb Haider, Yang-Er Chen","doi":"10.1111/ppl.70493","DOIUrl":"https://doi.org/10.1111/ppl.70493","url":null,"abstract":"Cadmium telluride quantum dots (CdTe QDs) have been increasing in the environment because of their large application in solar panels and biological industries. However, the potential role and bioaccumulation behavior of CdTe QDs in plants are unknown. Herein, the toxicity of CdTe QDs on the growth and the underlying mechanisms were explored in rice. Compared with the control, chlorophyll, anthocyanin, and net photosynthetic rate (Pn) of seedlings decreased by 44.6%, 53.7%, and 71.2% under 4.8 mg L-1 CdTe QDs exposure, respectively. However, O2˙- and H2O2 content in the roots significantly increased by 2.2- and 30.8-fold under 4.8 mg L-1 CdTe QDs exposure relative to the control, respectively. Te and Cd content in the leaves and roots increased incrementally with CdTe QDs exposure. Fourier transform infrared spectrometer (FTIR) analysis showed that toxic Cd of CdTe QDs mainly bound with the functional group (-OH) on the cell surface in rice. Furthermore, the analysis of untargeted metabolomics indicated that CdTe QDs exposure greatly distorted the glycolysis pathway, the amino acid metabolism, and the tricarboxylic acid cycle (TCA cycle). Further experiments confirmed that the activities of GSH, GPX, MDHAR, APX, and DHAR were dramatically upregulated by 5.7%, 18.7%, 32.1%, 20.8%, and 17.3% in the presence of 5 mM cysteine (Cys) compared with the sole CdTe QDs exposure, respectively. Therefore, we proposed that cysteine metabolism plays a key role in mitigating CdTe QDs toxicity. The study also proposes a new understanding regarding the application of Cys in improving crop performance in the CdTe QDs contaminated soil.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70493"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Nitrogen Application and Root Biomass Modulates 2-Acetyl-1-Pyrroline Biosynthesis in Fragrant Rice. 氮素用量优化及根系生物量调控香稻2-乙酰基-1-吡咯啉合成

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70504

Siying Deng, Tantan Zhang, Xiaojuan Pu, Yiming Mai, Jiewen Zheng, Shenggang Pan, Zhaowen Mo

{"title":"Optimization of Nitrogen Application and Root Biomass Modulates 2-Acetyl-1-Pyrroline Biosynthesis in Fragrant Rice.","authors":"Siying Deng, Tantan Zhang, Xiaojuan Pu, Yiming Mai, Jiewen Zheng, Shenggang Pan, Zhaowen Mo","doi":"10.1111/ppl.70504","DOIUrl":"https://doi.org/10.1111/ppl.70504","url":null,"abstract":"The rice root system mediates nutrient uptake while adapting to tillage, management, and environmental changes. While optimized nitrogen (N) supply is known to enhance 2-acetyl-1-pyrroline (2-AP) biosynthesis in fragrant rice, the underlying mechanisms linking nitrogen availability, root development, and their combined effects on physiological processes and aroma formation remain unclear. To address this knowledge gap, we conducted a pot experiment employing two fragrant rice cultivars (Huahangxiangyinzhen and Qingxiangyou19xiang) under three nitrogen regimes (0, 1.5, and 3.0 g N pot-1, recorded as LN, MN, and HN, respectively), and the root zone was isolated by enclosing it in a specialized bag to set contrasting root growth conditions (root-restricted and root-unrestricted, recorded as RR and UR, respectively). The results showed that root restriction increased the average 2-AP content by 22.90% despite reducing root biomass by approximately 50%, compared to unrestricted roots. Under nitrogen-containing treatments, root-restricted and root-unrestricted rice showed, respectively, an average 6.43% and 5.23% higher 2-AP content compared to nitrogen-free treatment. The structural equation modeling analysis illustrated that nitrogen metabolism physiology and photosynthetic physiology directly or indirectly significantly affected the accumulation of 2-AP. Random forest analysis further revealed that physiological changes at maturation substantially contributed to 2-AP synthesis. Therefore, this study advances the theoretical understanding of how nutrient assimilation processes and root morphology interact to modulate 2-AP biosynthesis in scented rice cultivars.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70504"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Surface Flatness Variation in "Lingwuchangzao" Jujube: A Multi-Omics and Morphological Study. “灵武长枣”枣表面平整度变化机制：多组学和形态学研究。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70489

Jia-Dong Wang, Ying Wang, Xiao-Qin Liu, Gai-Er Yang, Xuan Zhang, Yun-Mao Li, Bin Cao, Xiang Li

{"title":"Mechanisms of Surface Flatness Variation in \"Lingwuchangzao\" Jujube: A Multi-Omics and Morphological Study.","authors":"Jia-Dong Wang, Ying Wang, Xiao-Qin Liu, Gai-Er Yang, Xuan Zhang, Yun-Mao Li, Bin Cao, Xiang Li","doi":"10.1111/ppl.70489","DOIUrl":"https://doi.org/10.1111/ppl.70489","url":null,"abstract":"Using high- and low-surface flatness fruits of Ziziphus jujuba Mill. cv. \"Lingwuchangzao\" at different developmental stages as test materials, this study examined the mechanisms underlying variations in fruit appearance and internal quality. The findings revealed significant differences between high-surface flatness (S fruits) and low-surface flatness (R fruits) throughout development. S fruits were glossy, smaller, and predominantly seedless, with lower single-fruit weight and thinner pericarp and pulp, while R fruits exhibited reduced glossiness, larger size, and deeper coloration. Transcriptome analysis identified key genes, including CER1 and FAR, involved in wax synthesis, influencing cuticular wax production on the fruit surface. Additionally, genes such as AUX/IAA affected epidermal cell development, leading to smaller and denser cells in R fruits, thereby impacting surface flatness. Collectively, genes regulating cell development and wax biosynthesis determined the glossiness of \"Lingwuchangzao\" jujube fruits. Metabolomic analysis identified 779 metabolites, with flavonoids constituting the most abundant class. Protein interaction network analysis highlighted the hub gene ABCG31, closely linked to wax synthesis, as a critical factor in fruit surface flatness and glossiness. This study elucidates the quality differences and molecular regulatory mechanisms between high- and low-surface flatness \"Lingwuchangzao\" jujube fruits, offering valuable insights for genetic improvement to enhance visual appeal and commercial value.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70489"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water as a Compass: Hydrostimulation-Triggered Aerial Root Growth in Phalaenopsis aphrodite. 水作为指南针：水刺激引发的蝴蝶兰气生根生长。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70505

Hua-Chen Chang, I-Chian Chen, Jhun-Chen Chen, Yueh-Ju Hou, Su-Chiung Fang

{"title":"Water as a Compass: Hydrostimulation-Triggered Aerial Root Growth in Phalaenopsis aphrodite.","authors":"Hua-Chen Chang, I-Chian Chen, Jhun-Chen Chen, Yueh-Ju Hou, Su-Chiung Fang","doi":"10.1111/ppl.70505","DOIUrl":"10.1111/ppl.70505","url":null,"abstract":"Epiphytic orchids have evolved specialized adaptive strategies, such as aerial roots with water-absorbing velamen tissues, to cope with water-scarce and nutrient-deficient habitats. Our previous study revealed that the aerial roots of the epiphytic orchid Phalaenopsis aphrodite lack a gravitropic response, raising the possibility that alternative tropic mechanisms may contribute to their adaptation. In this study, we examined the effects of light and moisture on aerial root growth in P. aphrodite. Surprisingly, we found that light had no effect on root growth orientation. In contrast, localized moisture gradients consistently directed the growth of young aerial roots, indicating a hydrotropic response. To explore the underlying regulatory mechanisms, we performed hormone profiling of hydrostimulated root tissues. Our data showed that indole-3-acetic acid (IAA), salicylic acid (SA), jasmonic acid (JA), and abscisic acid (ABA) levels did not differ significantly between the water-facing and air-facing sides of the roots, suggesting other mechanisms may regulate this hydrotropism. In summary, our findings demonstrate that hydrotropism, rather than phototropism or gravitropism, guides aerial root growth in P. aphrodite. This hydrotropic response may represent a key adaptation that enables epiphytic orchids to effectively acquire water in the forest canopy.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70505"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12421945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Insights Into Pathways Supporting Optimized Biological Nitrogen Fixation in Chickpea and Their Interaction With Disease Resistance Breeding. 鹰嘴豆优化生物固氮途径的遗传见解及其与抗病育种的相互作用。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70514

S L Bithell, M A Asif, J Chowdhury, A K Kamiri, F Snijders, S Harden, K L Plett, J M Plett

{"title":"Genetic Insights Into Pathways Supporting Optimized Biological Nitrogen Fixation in Chickpea and Their Interaction With Disease Resistance Breeding.","authors":"S L Bithell, M A Asif, J Chowdhury, A K Kamiri, F Snijders, S Harden, K L Plett, J M Plett","doi":"10.1111/ppl.70514","DOIUrl":"10.1111/ppl.70514","url":null,"abstract":"In chickpea (Cicer arietinum), a globally important grain legume, improvements in yield stability are required to address food security and agricultural land loss. One approach is to improve both nutrient acquisition through symbiosis with rhizobial bacteria and biotic stress resistance. To support the simultaneous selection of multiple beneficial traits, we sought to identify quantitative trait loci (QTL) and genes linked to improved plant-microbe symbiosis both under symbiosis-promotive growth conditions and when pathogens are present. Our aims were to use the chickpea-Mesorhizobium rhizobial model to identify QTL associated with biological nitrogen fixation (BNF) and nutrient acquisition and understand factors promotive of sustained BNF under biotic stress through the impact of Phytophthora root rot (PRR) on BNF across chickpea genotypes on host gene expression. Using two chickpea × C. echinospermum recombinant inbred line (RIL) populations, we identified QTL associated with BNF and several associated with macro- and micro-nutrient status of chickpea. From within a set of the most PRR-resistant RIL (n = 70), we successfully identified RIL with both high PRR resistance and N sourced from BNF. In conditions of the tripartite (host:rhizobia:pathogen) interaction, while there was no consistent pathogen impact on the abundance of Mesorhizobium in nodules, PRR-resistant genotypes maintained a higher activity of their N-assimilation genes, while susceptible genotypes repressed these genes. This improved understanding of the genetic support of BNF in chickpea will allow selection for material that maintains higher BNF and is more disease resistant, which together may improve yield stability in chickpea.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70514"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12443505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Nitrogen Responsiveness in Spikelet Photosynthetic Performance Between Yellow and Black Oat (Avena sativa) Lemmas. 黄燕麦和黑燕麦小穗光合性能氮响应性的比较分析。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70530

Yue Xu, Kun Yi, Shuangfeng Yang, Peisheng Mao, Liru Dou, Xu Han, Manli Li

{"title":"Comparative Analysis of Nitrogen Responsiveness in Spikelet Photosynthetic Performance Between Yellow and Black Oat (Avena sativa) Lemmas.","authors":"Yue Xu, Kun Yi, Shuangfeng Yang, Peisheng Mao, Liru Dou, Xu Han, Manli Li","doi":"10.1111/ppl.70530","DOIUrl":"https://doi.org/10.1111/ppl.70530","url":null,"abstract":"Spikelets play a crucial role in photosynthesis during seed formation. This study used two oat (Avena sativa) varieties with significantly different lemma colors, \"Challenger\" from Canada and \"Qinghai444\" from China, as experimental materials. Phenotypic, physiological, proteomic, and transcriptional analyses were conducted on oat glumes, lemmas, and paleas after nitrogen application during the grain-filling stage. Results indicated that glumes outperformed lemmas in photosynthetic efficiency. After nitrogen application, \"Challenger\" glumes exhibited increased stomatal area but decreased chlorophyll a content, maximum photochemical efficiency of photosystem II (Fv/fm), and the quantum yield of photosystem II in steady state (ΦPSII). Concurrently, chloroplast membrane structure was repaired, and the expression of CAO, PsbR, and genes encoding chlorophyll protein complexes (LHCs) was upregulated, enhancing net photosynthetic rate (Pn) and photosynthetic capacity. Conversely, \"Qinghai444\" glumes showed decreased stomatal area but increased chlorophyll a content, Fv/fm, ΦPSII, and non-photochemical quenching (NPQ). The chloroplast structure of glumes was improved, whereas that of the lemmas was damaged. The CP47 subunit of photosystem II (PSII) accumulated on the thylakoid lamella, and the expression of petA, PsbB, and PsbR genes was upregulated, with no change in Pn or photosynthetic capacity. This study revealed that photosynthetic responses to nitrogen varied among oat varieties and spikelet tissues, with \"Challenger\" showing more pronounced enhancements. The findings of this study elucidate the patterns of photosynthetic responses to nitrogen in oat spikelets, guiding nitrogen fertilizer use and supporting the breeding of high-yielding oat varieties.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70530"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond Structure: The Role of the Outer Integument in Embryo, Endosperm, and Seed Development in Annona. 结构之外：外皮在番荔枝胚、胚乳和种子发育中的作用。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70538

María Garcia-Lezama, José I Hormaza, Enrique Lopez-Gomez, Noé Fernandez-Pozo, Jorge Lora

{"title":"Beyond Structure: The Role of the Outer Integument in Embryo, Endosperm, and Seed Development in Annona.","authors":"María Garcia-Lezama, José I Hormaza, Enrique Lopez-Gomez, Noé Fernandez-Pozo, Jorge Lora","doi":"10.1111/ppl.70538","DOIUrl":"10.1111/ppl.70538","url":null,"abstract":"In most angiosperms, the embryo develops within maternal tissues and is enclosed by two integuments, which play a crucial role in embryo and seed development. However, while this role has been extensively studied in model eudicots like Arabidopsis thaliana, it remains understudied in non-model plants and largely unexplored in basal and early-divergent angiosperms. Here, we examine the role of the outer integument in embryo development and seed formation using the unique spontaneous mutant \"Thai Seedless\" of the early-divergent angiosperm Annona squamosa (Annonaceae). This mutant lacks the outer integument due to a deletion in the INNER NO OUTER (INO) gene, leading to seed abortion. Comparative histological and transcriptomic analyses between the Annona ino mutant and wild-type Annona show that, unlike the polar growth observed in the nucellus of the wild-type genotype, Annona ino exhibited lateral nucellus expansion. Gene ontology (GO) enrichment revealed increased expression of reproductive development genes in wild-type seeds, while Annona ino showed altered gene expression patterns associated with cell death. These findings highlight both morphological and transcriptomic differences between Annona ino and wild-type genotypes, demonstrating that the absence of the outer integument disrupts early seed morphology and gene regulation, providing new insights into the genetic and developmental roles of integuments in seed development.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70538"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0