Identification and Functional Analysis of the Fragrance Terpene Synthase Gene PpTPS5 in Pyrethrum parthenium.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Fang Wang, Daihao Li, Sixian Zeng, Xueqian Gao, Guilan Wu, Di Huang, Zihan Cao, Qin Wang, Rangcai Yu, Yanping Fan
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引用次数: 0

Abstract

Pyrethrum parthenium, a perennial herb from the genus Pyrethrum within the Asteraceae family, is known for its potent fragrance and significant economic value. Nevertheless, the mechanisms involved in the synthesis and regulation of its floral fragrance compounds remain unclear. This study used gas chromatography-mass spectrometry (GC-MS) to analyze the volatile compounds in five parts of P. parthenium: tubular flowers, ligulate flowers, sepals, receptacles, and leaves. The results showed that the volatile aromatic components include terpenes such as camphor, α-pinene, camphene, d-limonene, β-caryophyllene, and β-farnesene, with camphor being the main volatile compound. Based on the transcriptome data of tubular flowers of P. parthenium, a terpene synthase gene, PpTPS5, was identified. The results of both in vitro and in vivo enzymatic assays demonstrated that PpTPS5 functions as a bifunctional terpene synthase gene. The qRT-PCR results showed that the gene expression pattern of PpTPS5 is correlated with the release pattern of the corresponding terpene aromatic compounds in P. parthenium. Subcellular localization and inhibitor experiments indicated that PpTPS5 functions in the cytosol. Additionally, dual-luciferase and electrophoretic mobility shift assay (EMSA) assays revealed that PpMYB9 can bind to the promoter of PpTPS5 to regulate the biosynthesis of terpenoids. In conclusion, the results of this study provide a theoretical basis for further exploration of the transcriptional regulation of terpene floral fragrance compounds in P. parthenium.

除虫菊香精萜合成酶基因PpTPS5的鉴定与功能分析。
除虫菊是菊科除虫菊属的一种多年生草本植物,以其浓烈的香味和显著的经济价值而闻名。然而,其花香化合物的合成和调控机制尚不清楚。本研究采用气相色谱-质谱联用技术(GC-MS)分析了parthenium的管状花、舌状花、萼片、花托和叶片五个部分的挥发性成分。结果表明,其挥发性芳香成分主要为樟脑、α-蒎烯、樟烯、d-柠檬烯、β-石竹烯、β-法尼烯等萜类化合物,其中以樟脑为主要挥发成分。利用parthenium管状花的转录组数据,鉴定了一个萜烯合成酶基因PpTPS5。体外和体内酶促实验结果表明,PpTPS5具有双功能萜烯合成酶基因的功能。qRT-PCR结果显示,PpTPS5基因表达模式与parthenium中相应萜类芳香族化合物的释放模式相关。亚细胞定位和抑制剂实验表明PpTPS5在细胞质中起作用。此外,双荧光素酶和电泳迁移迁移试验(EMSA)显示PpMYB9可以结合PpTPS5的启动子来调节萜类化合物的生物合成。综上所述,本研究结果为进一步探索parthenium中萜类花香化合物的转录调控提供了理论基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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