Physiologia plantarum最新文献_第4页

Beyond Structure: The Role of the Outer Integument in Embryo, Endosperm, and Seed Development in Annona. 结构之外：外皮在番荔枝胚、胚乳和种子发育中的作用。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70538

María Garcia-Lezama, José I Hormaza, Enrique Lopez-Gomez, Noé Fernandez-Pozo, Jorge Lora

{"title":"Beyond Structure: The Role of the Outer Integument in Embryo, Endosperm, and Seed Development in Annona.","authors":"María Garcia-Lezama, José I Hormaza, Enrique Lopez-Gomez, Noé Fernandez-Pozo, Jorge Lora","doi":"10.1111/ppl.70538","DOIUrl":"10.1111/ppl.70538","url":null,"abstract":"In most angiosperms, the embryo develops within maternal tissues and is enclosed by two integuments, which play a crucial role in embryo and seed development. However, while this role has been extensively studied in model eudicots like Arabidopsis thaliana, it remains understudied in non-model plants and largely unexplored in basal and early-divergent angiosperms. Here, we examine the role of the outer integument in embryo development and seed formation using the unique spontaneous mutant \"Thai Seedless\" of the early-divergent angiosperm Annona squamosa (Annonaceae). This mutant lacks the outer integument due to a deletion in the INNER NO OUTER (INO) gene, leading to seed abortion. Comparative histological and transcriptomic analyses between the Annona ino mutant and wild-type Annona show that, unlike the polar growth observed in the nucellus of the wild-type genotype, Annona ino exhibited lateral nucellus expansion. Gene ontology (GO) enrichment revealed increased expression of reproductive development genes in wild-type seeds, while Annona ino showed altered gene expression patterns associated with cell death. These findings highlight both morphological and transcriptomic differences between Annona ino and wild-type genotypes, demonstrating that the absence of the outer integument disrupts early seed morphology and gene regulation, providing new insights into the genetic and developmental roles of integuments in seed development.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70538"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding Plant Metabolomic Response to Potassium and Nutrient Stresses in Controlled Environments. 解码受控环境下植物对钾和养分胁迫的代谢组学反应。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70547

Md Mazadul Islam, Li Li, Jing He, Afroz Naznin, Samsul Huda, Talaat Ahemd, David Tissue, Zhong-Hua Chen

{"title":"Decoding Plant Metabolomic Response to Potassium and Nutrient Stresses in Controlled Environments.","authors":"Md Mazadul Islam, Li Li, Jing He, Afroz Naznin, Samsul Huda, Talaat Ahemd, David Tissue, Zhong-Hua Chen","doi":"10.1111/ppl.70547","DOIUrl":"10.1111/ppl.70547","url":null,"abstract":"Potassium (K) is an essential macronutrient, affecting numerous physiological, biochemical, and metabolic functions in plants. In protected cropping systems (PCS), which are controlled environments for intensive agriculture, optimizing K management is essential for attaining sustainable productivity and resilience under stressful growth conditions. Understanding plant responses to these conditions requires advanced analytical approaches, and metabolomics is emerging as a key tool, though there is still a lack of PCS-focused metabolomic studies. This review synthesizes the recent knowledge on plant metabolomic responses to K as a nutrient, emphasizing the central role of metabolomics in uncovering intricate biochemical pathways associated with K uptake, transport, and utilization. We investigate essential metabolic alterations in response to K deficiency, encompassing modifications in glucose metabolism, antioxidant synthesis, osmolyte accumulation, and hormonal regulation. The review also explores the relationships between potassium and other nutrients, specifically nitrogen, phosphorus, and essential micronutrients, and their impact on total plant metabolic networks. Furthermore, we discuss cutting-edge omics integration, precision fertigation, real-time sensor technologies, and machine learning applications that together promise to transform K fertilizer management in PCS. Future directions highlight the advancement of K-efficient cultivars, integrating metabolomic biomarkers in breeding, and overcoming challenges in data interpretation, scalability, and the high cost of metabolomic analyses and phenotyping technologies. Collectively, these insights provide a framework for improving crop health, production, and nutrient utilization efficiency for a more sustainable future in protected cropping.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70547"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BRASSINOSTEROID-SIGNALING KINASEs: Small Family, Big Functions. 油菜素类固醇信号激酶：小家族，大功能。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70555

Tian Zhao, Xiaoman Cao, Seçkin Eroğlu, Yiji Xia, Ahmed ElGamal, Hayam S Abdelkader, Jinglan Liu, Yiping Wang

{"title":"BRASSINOSTEROID-SIGNALING KINASEs: Small Family, Big Functions.","authors":"Tian Zhao, Xiaoman Cao, Seçkin Eroğlu, Yiji Xia, Ahmed ElGamal, Hayam S Abdelkader, Jinglan Liu, Yiping Wang","doi":"10.1111/ppl.70555","DOIUrl":"https://doi.org/10.1111/ppl.70555","url":null,"abstract":"Plant cells utilize cell-surface receptors to detect external cues or endogenous signals, thereby triggering appropriate responses that regulate various physiological processes. Receptor-like kinases (RLKs), located on the cell surface, play a pivotal role in recognizing extracellular signal molecules and activating downstream signaling pathways. Receptor-like cytoplasmic kinases (RLCKs), lacking extracellular ligand-binding domains, function as downstream partners of RLKs to transduce the signals to intracellular signaling components such as the mitogen-activated protein kinase (MAPK) cascade. RLCKs are classified into 17 subfamilies, which are further divided into distinct branches based on sequence similarity. Among the RLCKs, the XII-1 members, known as BRASSINOSTEROID-SIGNALING KINASEs (BSKs), play key roles in regulating diverse physiological processes. Given their importance, this review highlights recent advances in understanding the role of BSKs in plant growth, development, and stress responses. We detail the regulation of BSKs' subcellular localization through post-translational modifications and explore the molecular mechanisms by which BSKs modulate signaling pathways by engaging with RLK receptors and MAPK cascades. We also discuss the other signaling pathways regulated by BSKs. Collectively, this review underscores the role of BSKs as potential linker proteins localized at the plasma membrane (PM), functioning downstream of cell surface receptors and upstream of MAPK cascades and other signaling components to orchestrate a multitude of developmental processes and stress responses.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70555"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Non-Pathogenic Strain Fusarium oxysporum FO12 Mitigates Simultaneous Verticillium dahliae and Iron Deficiency Stresses in Arabidopsis thaliana. 非致病性菌株尖孢镰刀菌FO12减轻拟南芥中大丽花黄萎病和缺铁胁迫。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70550

Jesús Sevillano-Caño, Clara Córdoba-Galván, Antonio Rafael Sánchez-Rodríguez, Francisco J Romera, Antonio Trapero, María J García-Del Rosal, Carlos Agustí-Brisach

{"title":"The Non-Pathogenic Strain Fusarium oxysporum FO12 Mitigates Simultaneous Verticillium dahliae and Iron Deficiency Stresses in Arabidopsis thaliana.","authors":"Jesús Sevillano-Caño, Clara Córdoba-Galván, Antonio Rafael Sánchez-Rodríguez, Francisco J Romera, Antonio Trapero, María J García-Del Rosal, Carlos Agustí-Brisach","doi":"10.1111/ppl.70550","DOIUrl":"10.1111/ppl.70550","url":null,"abstract":"Verticillium wilt, caused by the soilborne fungus Verticillium dahliae, and iron (Fe) deficiency are major stresses impacting valuable crops in the Mediterranean basin. The plant response to these stresses consists of overlapping pathways that, coupled with the emerging use of endophytic fungi as biological control agents (BCAs) and plant biostimulants, enables the study of their potential to mitigate co-occurring biotic stresses and nutritional deficiencies such as Fe deficiency. To this end, Arabidopsis thaliana plants of the \"Columbia\" ecotype grown in calcareous soil were inoculated with the non-pathogenic strain Fusarium oxysporum FO12 and/or with the defoliating pathotype of V. dahliae strain V180. Plant growth, plant mineral uptake, and disease progression, along with the molecular quantification of FO12 and V180 fungal biomass and the relative expression of ISR-, SAR-, and Fe deficiency-related genes in shoots, were evaluated. The results revealed that plants inoculated with the FO12 strain increased growth, leaf chlorophyll concentration (SPAD index), and phosphorus (P; 126%) and Fe (9%) contents, partially due to ethylene (ET) and Iron Man peptides (IMA). In addition, FO12 reduced the severity of symptoms by more than 85%. This reduction in disease severity may be attributed to a direct competitive effect on V180 progression and to the induction of several defence-associated genes related to ET and SA synthesis and signaling. This bidirectional crosstalk between Fe deficiency responses and plant defences triggered by FO12 mainly through ET can improve plant resilience while reducing reliance on agrochemicals, offering a sustainable multifaceted tool for crop protection and nutrition management.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70550"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12485298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Metabolomics and Transcriptomics Resource for Identifying Candidate Genes in the Biosynthesis of Specialised Metabolites in Populus tremula. 白杨特化代谢物生物合成候选基因的代谢组学和转录组学资源。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70567

Sara M Rydman, Jenna Lihavainen, Kathryn M Robinson, Stefan Jansson, Benedicte R Albrectsen, Nathaniel R Street

{"title":"A Metabolomics and Transcriptomics Resource for Identifying Candidate Genes in the Biosynthesis of Specialised Metabolites in Populus tremula.","authors":"Sara M Rydman, Jenna Lihavainen, Kathryn M Robinson, Stefan Jansson, Benedicte R Albrectsen, Nathaniel R Street","doi":"10.1111/ppl.70567","DOIUrl":"10.1111/ppl.70567","url":null,"abstract":"This study aims to identify candidate genes involved in the biosynthesis of salicinoid phenolic glycosides (SPGs), a group of specialised metabolites characteristic of the Salicaceae family. While the integration of multi-omics data represents a powerful approach to link genes encoding enzymes and their regulatory factors to metabolite biosynthesis, suitable multi-omics data resources are scarce. We present a comprehensive dataset comprising untargeted liquid chromatography-mass spectrometry (LC-MS) and mRNA-sequencing data from various organs of European aspen (Populus tremula L.) and from genotypes that produce contrasting sets of SPGs. We present a reproducible pipeline for the analysis of the LC-MS data, including predicted annotation of potential novel SPGs. We demonstrate the utility of the resource by identifying candidate genes involved in the biosynthesis of SPGs with a cinnamoyl moiety. By integrating gene and metabolite differential analyses with a gene co-expression network, we identified two HXXXD-type acyltransferase genes and one UDP-glucosyltransferase gene as candidates for future downstream characterisation. The combined gene expression and metabolomics resource is integrated into PlantGenIE.org to facilitate easy access and data mining. All raw data are available in public databases, and all data and results files are available at an associated Figshare repository.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70567"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12508522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-Wide Identification of R2R3-MYB Gene Family in Fagopyrum dibotrys and Functional Characterization of FdMYB7 and FdMYB23. 荞麦R2R3-MYB基因家族的全基因组鉴定及FdMYB7和FdMYB23的功能鉴定

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70540

Nan Ma, Yazhu Wu, Muzi Li, Yu Cao, Zhongnian Zhang, Rui Qin, JiaHao Wen, Yeqin Xu, Chengcheng Bao, Qiaojun Jia, Dekai Wang

{"title":"Genome-Wide Identification of R2R3-MYB Gene Family in Fagopyrum dibotrys and Functional Characterization of FdMYB7 and FdMYB23.","authors":"Nan Ma, Yazhu Wu, Muzi Li, Yu Cao, Zhongnian Zhang, Rui Qin, JiaHao Wen, Yeqin Xu, Chengcheng Bao, Qiaojun Jia, Dekai Wang","doi":"10.1111/ppl.70540","DOIUrl":"https://doi.org/10.1111/ppl.70540","url":null,"abstract":"Fagopyrum dibotrys, an important medicinal and edible plant, is abundant in flavonoids, proanthocyanidins (PAs), and other bioactive substances showing a variety of pharmacological effects. The MYB transcription factors are involved in the biosynthesis regulation of various secondary metabolites. However, the knowledge of their biological functions in F. dibotrys is still very limited. In this study, a total of 112 FdR2R3-MYBs were identified, and the evolutionary relationships, chromosomal locations, conserved motifs, and protein structures were systematically analyzed. Tandem and segmental duplication facilitated the expansion of FdR2R3-MYB genes, in which segmental duplication played a major role. According to phylogenetic analysis and expression analysis, FdMYB7 and FdMYB23 were selected for further functional research. Transient expression analysis of tobacco leaf mesophyll cells showed that FdMYB7 and FdMYB23 proteins were located to the nucleus. The contents of total flavonoids and PA in Arabidopsis thaliana overexpressing FdMYB7 and FdMYB23 genes increased significantly. Moreover, several key genes involved in flavonoid and PA biosynthesis were significantly up-regulated. These findings contribute to understanding the biological activities of the FdR2R3-MYBs in the synthesis of flavonoids and PAs in F. dibotrys.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70540"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Increase in the Proportion of β-Diketone Epicuticular Wax Is a Beneficial Variant Trait for Drought Tolerance in Leymus chinensis. 羊草表皮蜡β-二酮比例增加是一种有益的耐旱变异性状。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70496

Mengtong Sun, Guangyue Tan, Xuefei Hu, Jing Wu, Heyao Wang, Shuang Feng, Xu Zhuang, Yue Wang, Aimin Zhou

{"title":"An Increase in the Proportion of β-Diketone Epicuticular Wax Is a Beneficial Variant Trait for Drought Tolerance in Leymus chinensis.","authors":"Mengtong Sun, Guangyue Tan, Xuefei Hu, Jing Wu, Heyao Wang, Shuang Feng, Xu Zhuang, Yue Wang, Aimin Zhou","doi":"10.1111/ppl.70496","DOIUrl":"10.1111/ppl.70496","url":null,"abstract":"Leymus chinensis is a perennial grass with remarkable adaptability and forage quality. It is the dominant species on the saline-alkali land in the Songnen Plain in Northeast China, where two ecotypes naturally grow: the grey-green (GG) and yellow-green (YG) genotypes, named after the leaf color. However, the differences in morphology and adaptability between the GG and YG ecotypes are not elucidated. In this study, we investigated and compared GG and YG ecotypes' cuticular wax and its composition. Furthermore, the difference in drought tolerance between the leaves of the two ecotypes was analyzed. Our results showed that GG plants have stronger drought tolerance owing to an optimal limiting non-stomatal water loss effect than the YG plants, which is associated with their higher proportion of tubular epicuticular wax. The composition of the tubular wax was mainly β-diketone 14,16-Hentriacontanedione (C31H60O2), and its biosynthesis may be closely related to the endoplasmic reticulum-localized diketone metabolism type-III polyketide synthase (DMP). Our findings suggest that the increased proportion of β-diketone epicuticular wax may be a beneficial variant trait for L. chinensis to optimally adapt to drought environments.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70496"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Translocation of Foliar-Applied Nanoparticles: A Critical Review From a Plant Science Perspective. 叶面应用纳米颗粒的易位：从植物科学的角度进行评述。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70476

Andrea Pinna, Søren Husted

{"title":"Translocation of Foliar-Applied Nanoparticles: A Critical Review From a Plant Science Perspective.","authors":"Andrea Pinna, Søren Husted","doi":"10.1111/ppl.70476","DOIUrl":"https://doi.org/10.1111/ppl.70476","url":null,"abstract":"Recent advances in plant science have greatly enhanced agronomic practices involving the foliar application of agrochemicals such as fertilizers and pesticides. However, the limited phloem mobility of certain nutrients and nonsystemic pesticides reduces the effectiveness of these strategies. Nanoparticles (NPs) have emerged as promising carriers to improve nutrient use efficiency (NUE) and crop protection by enabling long-distance transport and targeted delivery of essential nutrients and active ingredients. While increasing evidence suggests that foliar-applied NPs can translocate within plants, a mechanistic understanding and agronomically relevant case studies remain scarce. As a result, the extent to which NP translocation can facilitate significant distribution and cargo release within plants is yet to be fully established. This review critically evaluates existing research on foliar NP translocation, emphasizing key findings within a plant science framework. Specifically, we examine how NP design can influence translocation and assess the existing quantitative data of NP remobilization within plants. Additionally, we explore how physiological processes affect NP transport and highlight alternative, often overlooked translocation pathways. Lastly, we assess current techniques used to study NP transport, discussing their applicability and limitations. This review identifies significant research gaps that must be addressed to advance nano-enabled plant nutrition as well as crop protection and can therefore be used to inspire future research.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70476"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-Wide Identification and Evolution of Core Cell Cycle Genes in Marchantia polymorpha: Insights Into Redundancy, Stress, and Functional Evolution. 多形地豆核心细胞周期基因的全基因组鉴定和进化：对冗余、应激和功能进化的见解。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70485

Xingguang Chen, Haoran Feng, Mengjuan Liu, Jiahao Cai, Rabia Sarwar, Xueli Li, Mingyue Zhang, Xinyu Li, Xinqiang Lin, Zhonghua Guo, Jinbin Hu, Shuqi Yang, Lulu Wang, Xiaoping Niu, Gang Wang, Boping Tang, Sheng Wang, Yuan Qin, Yan Cheng

{"title":"Genome-Wide Identification and Evolution of Core Cell Cycle Genes in Marchantia polymorpha: Insights Into Redundancy, Stress, and Functional Evolution.","authors":"Xingguang Chen, Haoran Feng, Mengjuan Liu, Jiahao Cai, Rabia Sarwar, Xueli Li, Mingyue Zhang, Xinyu Li, Xinqiang Lin, Zhonghua Guo, Jinbin Hu, Shuqi Yang, Lulu Wang, Xiaoping Niu, Gang Wang, Boping Tang, Sheng Wang, Yuan Qin, Yan Cheng","doi":"10.1111/ppl.70485","DOIUrl":"https://doi.org/10.1111/ppl.70485","url":null,"abstract":"The cell cycle is a fundamental process of plant growth, development, and reproduction, in which cyclin-dependent kinases (CDKs) and cyclins (CYCs) play central roles in regulating the progression through various stages. These proteins are coordinated with multiple interacting partners to ensure the accurate execution of essential biological events such as DNA replication, chromosome segregation, and cell division. Marchantia polymorpha, one of the earliest diverging land plant species, has emerged as a key model for exploring fundamental mechanisms in plant biology and evolution. However, compared with other model plants, such as Arabidopsis thaliana and Oryza sativa, the core cell cycle genes in M. polymorpha remain relatively uncharacterized. In this study, we identified 31 core cell cycle genes in M. polymorpha through genome-wide analysis, including 13 CDKs, 8 CYCs, 5 E2F/DPs, 1 ICK, 1 RB, 1 CKS, and 2 Wee1 genes. We further analyzed their physicochemical properties, gene structures, and conserved domains, along with evolutionary pressures assessed via Ka/Ks and 4DTv analyses. Comparative genomic analysis revealed patterns of gene contraction and expansion. Additionally, we predicted cis-acting regulatory elements and performed differential expression analysis under various stress conditions to explore their potential functions and expression profiles. Finally, a protein-protein interaction (PPI) network was constructed, and key genes were experimentally validated. These findings provide valuable insights into the core cell cycle gene family in M. polymorpha, contributing to an enhanced understanding of cell cycle regulation and its evolutionary significance in plants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70485"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Significant circRNAs, microRNAs, and Target Genes Participate in the Rooting Process of Acer truncatum. 重要环状rna、微小rna和靶基因参与槭根生根过程。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70510

Jiayu Yu, Jiaming Qin, Junjie Wang, Kezhong Zhang, Wei Ge

{"title":"Significant circRNAs, microRNAs, and Target Genes Participate in the Rooting Process of Acer truncatum.","authors":"Jiayu Yu, Jiaming Qin, Junjie Wang, Kezhong Zhang, Wei Ge","doi":"10.1111/ppl.70510","DOIUrl":"10.1111/ppl.70510","url":null,"abstract":"Acer truncatum is a multifunctional tree species widely planted worldwide with extremely high commercial value. It is not easy to take root under natural conditions, and plant growth hormone needs to be used to promote the formation of adventitious roots in stem cuttings. This study aimed to determine how non-coding RNAs and mRNAs regulate the rooting process of A. truncatum in the mode of competitive endogenous RNA interactions. Based on whole transcriptome analysis of the control and treatment (500 mg/L IBA for 30 min) groups, 133 differentially expressed mRNAs, 58 differentially expressed miRNAs, 81 differentially expressed lncRNAs, and 3 differentially expressed circRNAs were selected. Among the differentially expressed miRNAs, 34 differentially expressed miRNAs can target 100 genes. Moreover, 2105 circRNAs were identified, of which 145 interacted with rooting-related miR160, miR164, and miR171. Finally, the ciRNA46-miR164b-NAC1 regulatory network was selected. Real-time quantitative polymerase chain reaction, dual luciferase assays, and β-glucuronidase gene tissue staining experiments verified the interaction among ciRNA46-miR164b-NAC1. Overexpression experiments showed that NAC1 promoted the development of adventitious roots, whereas miR164b inhibited their development. Bimolecular fluorescence complementation and yeast two-hybrid revealed the interaction of NAC1 with SHORT-ROOT. These results explain the mechanism of action in the rooting process of A. truncatum, offering a scientific foundation for further research on its molecular mechanisms during rooting.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70510"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0