Physiologia plantarum最新文献_第4页

Modelling photosynthetic responses by day and night during initial water stress in Pulmonaria vallarsae.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.70004

Paolo Pupillo, Francesca Sparla, Bruno Andrea Melandri, Paolo Trost

{"title":"Modelling photosynthetic responses by day and night during initial water stress in Pulmonaria vallarsae.","authors":"Paolo Pupillo, Francesca Sparla, Bruno Andrea Melandri, Paolo Trost","doi":"10.1111/ppl.70004","DOIUrl":"10.1111/ppl.70004","url":null,"abstract":"The relationships between photosynthesis and initial water deficit stress were investigated by chlorophyll fluorescence analysis in Pulmonaria vallarsae, a shade tolerant, perennial C3 herb by following changes of light response curves (LRCs) in single leaves during water shortage. We devised an LRC model based on two interacting rectangular hyperbolae (DH model) for the low (H1) and the high irradiance regions (H2), characterized by two parameters: maximum extrapolated ETR (V1, V2) and half-saturation irradiance (K1, K2). While H1 is assumed to represent an ETR-related function, H2 may reflect Rubisco activity. Plants were subjected to four days of water restriction in summer and tested every 12 h. While daytime values remained relatively stable, increasing water stress gradually induced a night depression of photosynthesis mainly dependent on decreasing ETR with concomitant reduction of PSII-dependent parameters (ΦPSII) and fluorescence-related functions, while nonphotochemical quenching (NPQ) was strongly enhanced. In terms of the DH model, the night depression of photosynthesis featured a night drop of V2 and K2 followed by decreases of V1 and K1. The H2 hyperbola was more stress responsive than H1 and frequently showed a reversible decrease of nocturnal H2 parameters (bright illumination constraint, BIC). Pulmonaria plants tested during winter with very low water stress displayed LRCs resembling rectangular hyperbolae, similar during day and night. The DH model is shown to yield accurate and perspicuous photosynthetic parameters representing the principal components of an LRC and to be well suitable to document the day/night divergence of photosynthetic capacity during a water deficit stress.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e70004"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11632138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of early transcriptomic changes to diverse microbial volatiles in Arabidopsis thaliana.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.70002

Ching-Han Chang, Chung-Chih Huang, Pei-Yu Su, Yi-Rong Li, Yu-Shuo Chen, Chong-Yue Wang, Yuan-Yun Zhang, Hieng-Ming Ting, Hao-Jen Huang

{"title":"Comparison of early transcriptomic changes to diverse microbial volatiles in Arabidopsis thaliana.","authors":"Ching-Han Chang, Chung-Chih Huang, Pei-Yu Su, Yi-Rong Li, Yu-Shuo Chen, Chong-Yue Wang, Yuan-Yun Zhang, Hieng-Ming Ting, Hao-Jen Huang","doi":"10.1111/ppl.70002","DOIUrl":"https://doi.org/10.1111/ppl.70002","url":null,"abstract":"Microbial volatiles organic compounds (mVOCs) play diverse roles in modulating plant growth and stress tolerance. However, the molecular responses of plants to mVOCs are largely undescribed. In this study, we examined the early transcriptomic response of Arabidopsis thaliana to two plant growth-promoting mVOCs (PGPVs) and one plant growth-inhibiting mVOC (PGIV). Our phenotype analysis showed that PGPVs from Fusarium verticillioides and Simplicillium sympodiophorum promote plant growth by affecting different organs. In particular, F. verticillioides mVOCs promote plant growth in whole seedlings, while S. sympodiophorum mVOCs increase leaf surface area. Moreover, Arabidopsis treated with the two PGPVs exhibited different growth-associated molecular responses, which corresponded to the phenotype analysis results. For instance, the FAR1 family (regulates light-associated plant development) was upregulated by F. verticillioides mVOCs, while the LBD family (regulates leaf size and shape) was enriched among S. sympodiophorum mVOC-upregulated genes. Hierarchical clustering analysis further indicated that PGPVs induced expression of growth-associated genes and suppressed expression of defense-associated genes. In contrast to the PGPV-induced transcriptional effects, PGIVs caused downregulation of growth-associated genes with coincident upregulation of defense-associated genes. Furthermore, a transcription factor (TF) enrichment analysis suggested that HSFs, NACs and WRKYs might be core regulators in the plant response towards mVOCs. In particular, WRKYs might serve as integrating nodes to regulate salicylic acid- and jasmonic acid-mediated defense responses and growth-defense trade-offs. Overall, this study provides insights into the early molecular responses of plants after mVOC exposure and suggests that these molecular responses contribute to different phenotypic responses.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e70002"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Etiolation promotes protoplast transfection and genome editing efficiency. 蚀刻可提高原生质体转染和基因组编辑的效率。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14637

Yunsun Kim, Eunbin Lee, Beum-Chang Kang

{"title":"Etiolation promotes protoplast transfection and genome editing efficiency.","authors":"Yunsun Kim, Eunbin Lee, Beum-Chang Kang","doi":"10.1111/ppl.14637","DOIUrl":"https://doi.org/10.1111/ppl.14637","url":null,"abstract":"In plants, DNA-free genome editing using preassembled clustered regularly interspaced short palindromic repeats (CRISPR)-ribonucleoprotein (RNP) has the advantage of avoiding transgene integration and limiting off-target effects. The efficiency of this gene editing strategy can vary, so optimization of protoplast transfection conditions is necessary to achieve maximum yield. In this study, we examined the effects of etiolation, or increased exposure to darkness during cultivation, on the transfection efficiency of protoplasts from lettuce and Chinese cabbage. Seedlings were grown under three different conditions: non-etiolated, etiolated, and de-etiolated. First, we tested PEG-mediated transfection after etiolation using a plasmid DNA for green fluorescent protein (GFP)-expression. Etiolated protoplasts had the highest percentage of GFP-expressing cells, with a 3.1-fold and 4.8-fold improvement in lettuce and Chinese cabbage, respectively, compared with non-etiolated protoplasts. We also assessed gene editing of endogenous genes after etiolation using CRISPR-RNP. Using targeted deep sequencing, we observed the highest editing efficiency in etiolated protoplasts from both plant species, for the LsPDS and LsFT genes in lettuce, this led to an 8.7-fold and 4.4-fold improvement compared with non-etiolated protoplasts, respectively. These results suggest that etiolation during seedling growth can improve transfection efficiency and DNA-free gene editing in protoplasts.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14637"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Iron metabolism acts as a bridge between photosynthesis and red coloration of bud galls induced on Nothofagus obliqua (Nothofagaceae).

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14651

Ígor Abba Arriola, Lubia Maria Guedes, Ana Silvia Franco Pinheiro Moreira, Narciso Aguilera, Rosy Mary Dos Santos Isaias, Denis Coelho de Oliveira

{"title":"Iron metabolism acts as a bridge between photosynthesis and red coloration of bud galls induced on Nothofagus obliqua (Nothofagaceae).","authors":"Ígor Abba Arriola, Lubia Maria Guedes, Ana Silvia Franco Pinheiro Moreira, Narciso Aguilera, Rosy Mary Dos Santos Isaias, Denis Coelho de Oliveira","doi":"10.1111/ppl.14651","DOIUrl":"https://doi.org/10.1111/ppl.14651","url":null,"abstract":"Color and morphology are some of the most intriguing traits of plant galls, whose patterns resemble fruits and flowers. Many hypotheses were proposed to explain the involvement of anthocyanin accumulation with the development of red gall hues, whose mechanisms seem idiosyncratic. Anthocyanins are related to photoprotective strategies in green tissues and metal accumulation in some flowers. Despite that, the combination of such physiological phenomena has been neglected for galls, which are photosynthetic neoplasms genetically similar to reproductive organs. Here, we integrated different perspectives by measuring photosynthetic pigment and anthocyanin concentration combined with fluorescence quenching analysis, antioxidant activity assays, and histochemical elemental mapping in red and green galls induced by Espinosa nothofagi (Hymenoptera) on Nothofagus obliqua (Nothofagaceae). We found no relationship between high anthocyanin concentrations, light exposure, and red coloration in galls as anthocyanin concentrations were higher in the outermost tissues of green galls than in red galls. Red galls presented higher concentrations of total chlorophyll and lower carotenoid concentrations than green galls and leaves, which correlated with their highest photosynthetic activity and iron accumulation. The red color coincides with the accumulation of aluminum and Fe3+ and the lowest antioxidant capacity in the gall outer tissue. The high antioxidant capacity of N. obliqua galls and the Fe2+ and Fe3+ distribution are related to high photosynthesis, Fe-use efficiency in galls, and the supply of Fe to the inducer diet. Overall, iron metabolism connects the high photosynthesis activity to the red gall color in the presence of low anthocyanin concentrations, like some flowers.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14651"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of seed infection by Fusarium verticillioides on maize performance against Sesamia nonagrioides attack.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14649

N Gesteiro, A Cao, R Santiago, P Lobagueira, S J González-Prieto, R A Malvar, A Butrón

{"title":"Effects of seed infection by Fusarium verticillioides on maize performance against Sesamia nonagrioides attack.","authors":"N Gesteiro, A Cao, R Santiago, P Lobagueira, S J González-Prieto, R A Malvar, A Butrón","doi":"10.1111/ppl.14649","DOIUrl":"10.1111/ppl.14649","url":null,"abstract":"In maize (Zea mays L), the fungus Fusarium verticillioides can behave as a pathogen, but it is also able of asymptomatic colonization as an endophyte. Therefore, it would be of great value to identify metabolites and/or metabolic pathways implicated in mutualistic and pathogenic interactions. The objectives of the present study were: (i) to investigate the effect of seed colonization by F. verticillioides on maize growth in a group of inbreds with contrasting resistance to F. verticillioides; (ii) to know if maize priming by Fusarium seed infection affects maize response to other parasites and if these differences could depend on genotype resistance to Fusarium; and (iii) to determine which metabolites could be associated to beneficial/detrimental changes on maize performance. Targeted and untargeted metabolomic approaches were carried out to characterize the response of control and primed plants to the most common maize pest in the Mediterranean area, Sesamia nonagrioides Lefèbvre (Lepidoptera: Noctuidae). The study cannot assume a differential pattern of infection between resistant and susceptible inbreds, but seed inoculation with F. verticillioides upon infestation with S. nonagrioides, significantly altered defense metabolism in resistant inbreds. Meanwhile it also induced a lipid response in susceptible inbreds that could mediate their increased plant susceptibility to insect attack. Although an endophytic interaction between the fungus and specific genotypes cannot be proven, defense pathways were favorably altered by F. verticillioides colonization among resistant inbreds.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14649"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11615131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the role of growth regulators in enhancing plant immunity against herbivory. 解密生长调节剂在增强植物免疫力对抗食草动物方面的作用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14604

Archana Singh, Kalpesh Nath Yajnik, Kanakachari Mogilicherla, Indrakant K Singh

{"title":"Deciphering the role of growth regulators in enhancing plant immunity against herbivory.","authors":"Archana Singh, Kalpesh Nath Yajnik, Kanakachari Mogilicherla, Indrakant K Singh","doi":"10.1111/ppl.14604","DOIUrl":"https://doi.org/10.1111/ppl.14604","url":null,"abstract":"Plants are central to global food production, and the pursuit of sustainability aims to enhance or preserve food quality while safeguarding the environment. Due to their immobility, plants are unable to evade unfavourable climatic setups or interactions with other living creatures. Upon their interaction with insect herbivores, plants face biotic stress, which is a constant challenge for plants, causing molecular, physiological, and biochemical changes and reducing their productivity. To combat biotic stress caused by herbivores, plants have evolved intricate defence mechanisms through growth regulators such as auxins, cytokinins, gibberellins, salicylic acid (SA), jasmonic acid (JA), ethylene (ET), abscisic acid (ABA), strigolactones and brassinosteroids. The intricate network of specific proteins, metabolites and certain phytohormones orchestrates plant defensive reactions, leading to their skilful coordination in responding to insect attacks. Comprehending the defence mechanisms holds the key to mitigating significant crop and economic losses. This review entails a comprehensive analysis of the role of growth regulators in enhancing plant immunity against herbivory, highlighting the substantial efforts by the scientific community to manage and mitigate damages from biotic stress in plants, ultimately contributing to the advancement of sustainable agriculture.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14604"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142550082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Metabolomic and Transcriptomic Analysis Reveals Bioactive Compound Diversity in Organs of Saffron Flower. 综合代谢组学和转录组学分析揭示藏红花器官中生物活性化合物的多样性

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14598

Jayram Bagri, Vikash Kumar Singh, Khushboo Gupta, Jeremy Dkhar, Aijaz Ahmad Wani, Mukesh Jain, Sneh Lata Singla-Pareek, Ashwani Pareek

{"title":"Integrated Metabolomic and Transcriptomic Analysis Reveals Bioactive Compound Diversity in Organs of Saffron Flower.","authors":"Jayram Bagri, Vikash Kumar Singh, Khushboo Gupta, Jeremy Dkhar, Aijaz Ahmad Wani, Mukesh Jain, Sneh Lata Singla-Pareek, Ashwani Pareek","doi":"10.1111/ppl.14598","DOIUrl":"https://doi.org/10.1111/ppl.14598","url":null,"abstract":"Saffron stigma, derived from Crocus sativus L., has long been revered in global traditional medicine and continues to hold significant market value. However, despite the extensive focus on saffron stigma, the therapeutic potential of other floral components remains underexplored, primarily due to limited insights into their complex molecular architectures and chemical diversity. To address this gap, we performed a comprehensive metabolomic analysis of various floral organs utilizing advanced analytical platforms, including GC-MS and UPLC-MS/MS. This in-depth profiling revealed a diverse array of 248 metabolites, encompassing amino acids, sugar derivatives, fatty acids, flavonoids, vitamins, polyamines, organic acids, and a broad spectrum of secondary metabolites. Distinct correlation patterns among these metabolites were identified through PCA and PLS-DA, highlighting unique metabolomic signatures inherent to each floral organ. We further integrated these metabolomic findings with our transcriptomic data, enabling a detailed understanding of the molecular and metabolic variations across different floral organs. The pronounced abundance of differentially expressed genes and metabolites in the stamen (424), leaf (345), tepal (196), stigma (177), and corm (133) underscores the intricate regulatory networks governing source-to-sink partitioning and dynamic metabolic processes. Notably, our study identified several bioactive compounds, including crocin, picrocrocin, crocetin, safranal, cannabielsoin, quercetin, prenylnaringenin, isorhamnetin, pelargonidin, kaempferol, and gallic acid, all of which exhibit potential therapeutic properties. In conclusion, this comprehensive analysis significantly enhances our understanding of the molecular mechanisms driving the biosynthesis of apocarotenoids, cannabinoids, anthocyanins, and flavonoids in saffron, thereby providing valuable insights and paving the way for future research in this area.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14598"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytosolic fructose-1,6-bisphosphatase isoform mediates metabolic adjustments in bean fruit pericarp to support seed growth. 细胞膜果糖-1,6-二磷酸酶同工酶介导豆类果实果皮中的代谢调整，以支持种子生长。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14631

Lilia Bernal, Patricia Coello, Daniel Padilla-Chacón, Eleazar Martínez-Barajas

{"title":"Cytosolic fructose-1,6-bisphosphatase isoform mediates metabolic adjustments in bean fruit pericarp to support seed growth.","authors":"Lilia Bernal, Patricia Coello, Daniel Padilla-Chacón, Eleazar Martínez-Barajas","doi":"10.1111/ppl.14631","DOIUrl":"10.1111/ppl.14631","url":null,"abstract":"Seed development requires substantial metabolic resources and is influenced by adverse environmental conditions. However, the ability of plants to produce viable seeds under restrictive conditions suggests the existence of mechanisms that make this process less sensitive to environmental stress. Uncovering their regulation could lead to the development of genotypes better adapted to stressful conditions. Plant response to stress is complex, and the contribution of organs such as the fruit pericarp to stress tolerance mechanism may have been underestimated. The bean fruit pericarp, a photosynthetic structure that contributes to seed development, can synthesize starch from surplus sucrose, which is later degraded during the rapid seed growth phase. This metabolic flexibility may be crucial for supporting seed growth when the photosynthate supply is reduced. To explore this possibility, we disrupted phloem continuity at the pedicel level in fruits about to enter the seed reserve accumulation stage. We used the capacity of the pericarp to incorporate 14CO2 to investigate changes in its metabolism. Our findings reveal that, in response to reduced photosynthate availability, the fruit pericarp did not increase 14CO2 fixation. However, the amount of 14C used for starch synthesis decreased, while the proportion used for soluble sugars synthesis increased. This shift resulted in an increase in 14C-products transported to seeds was accompanied by a significant increase in the activity of cytosolic fructose 1,6-bisphosphatase. Our results indicate that photosynthate restriction accelerates the degradation of pericarp storage proteins, and the increase in cFBPase activity could be crucial in converting the carbon produced in carbohydrates.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14631"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and epigenetic control of dormancy transitions throughout the year in the monoecious cork oak. 雌雄同株栓皮栎全年休眠转换的遗传和表观遗传控制。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14620

Helena Gomes Silva, Rómulo Sobral, Ana Teresa Alhinho, Hugo Ricardo Afonso, Teresa Ribeiro, Patrícia M A Silva, Hassan Bousbaa, Leonor Morais-Cecílio, Maria Manuela Ribeiro Costa

{"title":"Genetic and epigenetic control of dormancy transitions throughout the year in the monoecious cork oak.","authors":"Helena Gomes Silva, Rómulo Sobral, Ana Teresa Alhinho, Hugo Ricardo Afonso, Teresa Ribeiro, Patrícia M A Silva, Hassan Bousbaa, Leonor Morais-Cecílio, Maria Manuela Ribeiro Costa","doi":"10.1111/ppl.14620","DOIUrl":"https://doi.org/10.1111/ppl.14620","url":null,"abstract":"Bud dormancy plays a vital role in flowering regulation and fruit production, being highly regulated by endogenous and environmental cues. Deployment of epigenetic modifications and differential gene expression control bud dormancy/break cycles. Information on how these genetic and epigenetic mechanisms are regulated throughout the year is still scarce for temperate trees such as Quercus suber. Here, the expression levels of CENTRORADIALIS-LIKE (CENL) and DORMANCY-ASSOCIATED PROTEIN 1 (QsDYL1) during seasonal cycles of bud development, suggesting that QsCENL may be implicated in growth cessation in Q. suber and that QsDYL1 is a good dormancy marker. As gene expression can be regulated by the activity of chromatin modifiers, we have analysed the expression of these genes and the deposition of epigenetic marks in dormant versus non-dormant bud meristems. The DNA methyl transferases CHROMOMEHTYLASE 3 (QsCMT3) and METHYLTRANSFERASE 1 (QsMET1) were more expressed in the transition between dormancy to bud swelling. QsCMT3 was also highly expressed during the late stages of active bud formation. Conversely, the HISTONE ACETYLTRANSFERASE 1 (QsHAC1) was up-regulated during growth cessation and dormancy when compared to bud swelling. These results indicate that epigenetic regulation is implicated in how bud development progresses in Q. suber, which can be observed in the different profile deposition of the repressive and active marks, 5mC and H3K18Ac/H3K4me, respectively. The identification of bud-specific genetic and epigenetic profiling opens new possibilities to predict the relative rate of dormancy/growth of the bud stages, providing tools to understand how trees respond to the current challenges posed by climate change.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14620"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MdILL6 regulates xylem and vessel development to control internode elongation in spur-type apple. MdILL6调节木质部和血管的发育，控制刺型苹果节间伸长。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2024-11-01 DOI: 10.1111/ppl.14613

Xiuhua Gao, Hongwei Sun, Yandong Liu, Shubiao Zhang, Yu Liu, Muhammad Mobeen Tahir, Lu Tong, Pan Zhang, Turgunbayev Kubanychbek Toktonazarovich, Yanrong Lv, Juanjuan Ma, Dong Zhang, Jiangping Mao

{"title":"MdILL6 regulates xylem and vessel development to control internode elongation in spur-type apple.","authors":"Xiuhua Gao, Hongwei Sun, Yandong Liu, Shubiao Zhang, Yu Liu, Muhammad Mobeen Tahir, Lu Tong, Pan Zhang, Turgunbayev Kubanychbek Toktonazarovich, Yanrong Lv, Juanjuan Ma, Dong Zhang, Jiangping Mao","doi":"10.1111/ppl.14613","DOIUrl":"https://doi.org/10.1111/ppl.14613","url":null,"abstract":"Spur-type varieties play an important role in facilitating high-density plantings. However, the underlying mechanisms of internode elongation in spur-type varieties are poorly understood. In this research, we investigated the morphological phenotype of annual shoots in four spur-type varieties ('Miyazak', 'Jinfu 18', 'Yanfu No. 6', and 'Liquan spur') and four standard-type varieties ('Aomorifu', 'Shou Fuji', 'Yanfu No. 10', and 'Yanfu No. 3'). Compared with standard-type varieties, spur-type varieties had a shorter shoot length, an average internode length and a smaller xylem size. The content of Jasmonic acid (JA) and Jasmonic acid isoleucine (JA-Ile) significantly increased in spur-type varieties, accompanied by an increase in the expression of JA biosynthesis and signal transduction genes. Exogenous methyl jasmonate (MeJA) inhibited plant height, xylem size, and vessel area. Additionally, we identified an IAA-Leucine Resistant1-like Hydrolase family member, MdILL6, which was highly expressed in spur-type varieties and mature stems. MdILL6 was mainly expressed in the shoot tips and stem, and its protein was located on the endoplasmic reticulum. Overexpression of MdILL6 in apple inhibited plant height and average internode length by decreasing xylem size and vessel area. Our results revealed a molecular mechanism of spur-type variety development affected by the JA pathway and suggest strategies for genetic improvement and regulation of spur-type varieties.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14613"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0