Physiologia plantarum最新文献

{"title":"Genome-Wide Analysis of WRKY Family Reveals Candidate Regulators of Endophyte Mediated Dendrobine Biosynthesis in Dendrobium nobile.","authors":"Lin Li, Nana Chen, Xinying Zhong, Yu Zeng, Delin Xu, Xingkai Zhan, Linshan Tang, Gang Qian, Dao Chen","doi":"10.1111/ppl.71107","DOIUrl":"https://doi.org/10.1111/ppl.71107","url":null,"abstract":"<p><p>WRKY transcription factors (TFs) are important regulators of plant growth, stress responses, and secondary metabolism; however, their roles in endophyte-mediated dendrobine biosynthesis remain largely unknown in Dendrobium nobile. Here, we identified 63 DnWRKY genes in the D. nobile genome and systematically investigated their potential involvement in endophyte-mediated dendrobine accumulation. Phylogenetic analysis revealed high conservation of DnWRKY proteins within subfamilies. Endophytic fungal co-culture significantly promoted dendrobine accumulation, reaching 0.39 mg g^-1 at 14 days, which was 2.6-fold higher than that of the control. Transcriptome and qRT-PCR analyses showed that several DnWRKY genes responded to fungal treatment and exhibited expression patterns consistent with dendrobine accumulation. Among them, DnWRKY18 and DnWRKY4 were significantly positively correlated with key enzyme genes involved in dendrobine biosynthesis, including HMGS, TPS1, and TPS2. Promoter analysis further identified typical W-box elements in these target genes, supporting the possibility of WRKY-mediated transcriptional regulation. These results provide the first evidence linking WRKY TFs to endophyte-induced dendrobine biosynthesis in D. nobile and suggest that DnWRKY18 and DnWRKY4 may serve as candidate positive regulators in this process, establishing a putative regulatory framework for further functional verification of WRKY-mediated dendrobine biosynthesis.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71107"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149170926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CpCBF6-Mediated Activation of Antioxidant Defense and Osmotic Adjustment Enhanced Cold Tolerance in Cerasus pseudocerasus Lindl.","authors":"Juan Fu, Tao Tang, Jinyu Wu, Miao Liu, Ying Yu, Qiye Yang, Tian Tian","doi":"10.1111/ppl.71115","DOIUrl":"https://doi.org/10.1111/ppl.71115","url":null,"abstract":"<p><p>Low-temperature stress restricts plant growth, development, and geographical distribution. The C-repeat binding factor (CBF) is a core transcriptional regulator in plant cold stress responses, but its role in the thermophilic \"Manaohong\" cherry (Cerasus pseudocerasus Lindl.) remains elusive. Here, a cold-induced transcription factor gene, CpCBF6, was isolated and characterized from this species. Phylogenetic analysis showed its protein shares the highest homology with orthologs from Prunus persica and Prunus dulcis in Rosaceae. CpCBF6 localized to the nucleus, and its transactivation function mainly depends on the N-terminal and C-terminal domains. Forward and reverse genetics confirmed that CpCBF6 positively regulates cold tolerance. Heterologous overexpression in Arabidopsis thaliana enhanced cold tolerance, while virus-induced gene silencing (VIGS)-mediated knockdown in \"Manaohong\" cherry increased cold sensitivity. Physiological and biochemical analyses revealed that CpCBF6 scavenges reactive oxygen species (ROS) by enhancing superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities, thereby reducing malondialdehyde (MDA) accumulation. Concurrently, CpCBF6 promoted the synthesis of soluble sugars, soluble proteins, and proline, alleviating oxidative and osmotic stress. Yeast one-hybrid and dual-luciferase assays demonstrated that CpCBF6 directly targets and activates promoters of the cold-responsive genes CpCOR27 and CpCOR47. This study provides the first systematic functional characterization of CpCBF6 in \"Manaohong\" cherry, elucidating its molecular mechanism in enhancing cold tolerance via activating CpCORs expression, strengthening antioxidant defense, and improving osmotic adjustment, thus offering a key candidate gene and theoretical foundation for cold tolerance breeding in fruit trees.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71115"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149063786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen Peroxide and MAPK-Associated Responses During Methyl Jasmonate-Induced Elicitation of Terpenoid Indole Alkaloid in Catharanthus roseus Callus.","authors":"Marwa Mostafa Rizk, Badreldin A Hamed, Emad A Alsherif, Shereen Magdy Korany, Nadia Mohamed El-Shafey","doi":"10.1111/ppl.71157","DOIUrl":"https://doi.org/10.1111/ppl.71157","url":null,"abstract":"<p><p>Methyl jasmonate (MeJA) is a commonly used elicitor for enhancing secondary metabolite production, including the anticancer terpenoid indole alkaloids (TIAs) vinblastine and vincristine. To investigate the physiological and molecular responses associated with MeJA-induced TIA accumulation, we examined the effect of different MeJA concentrations on vinblastine and vincristine production, gene expression and antioxidant defence in Catharanthus roseus calli. MeJA significantly enhanced relative growth and increased vinblastine and vincristine accumulation, with the highest increases observed at 50 μM MeJA, reaching 159.2% and 183.4% of control, respectively, and upregulated CrMPK3, STR, and DAT expression. The lower concentrations of MeJA showed higher H₂O₂ accumulation, DPPH free radical-scavenging and flavonoid content. Whereas increased lipid peroxidation and PAL enzyme activity were associated with the higher concentrations (100 and 200 μM). The upregulated expression of CrMPK3, together with the biosynthetic genes STR and DAT, supports the involvement of MAPK in signalling responses that mediate MeJA-elicited TIAs. Pearson correlation and principal component analysis showed that H₂O₂ accumulation was more closely associated with vinblastine and vincristine accumulation than with the expression of the investigated genes at the experimental time points. These findings suggest that H₂O₂ accumulation may be associated with downstream metabolic/redox processes accompanying alkaloid accumulation, although an earlier transient role of H₂O₂ in MeJA signalling cannot be excluded. The enhanced flavonoid accumulation and DPPH scavenging activity further suggest a role for antioxidant responses in maintaining redox homeostasis during MeJA-induced alkaloid accumulation.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71157"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149220603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in Foliar Water Uptake Caused by Water Stress in Cattleya Lindl. (Orchidaceae).","authors":"Jéssica Ferreira de Lima, Gisely de Souza Santos, Denis Coelho de Oliveira, Ana Silvia Franco Pinheiro Moreira","doi":"10.1111/ppl.71047","DOIUrl":"10.1111/ppl.71047","url":null,"abstract":"<p><p>In response to water restrictions imposed by the epiphytic environment, orchids have developed strategies to increase water-use efficiency, such as the expression of Crassulacean Acid Metabolism (CAM) and the foliar water uptake (FWU). In a climate change scenario, FWU may emerge as a strategy to reduce water stress during drought. In this study, we propose that, in Cattleya forbesii Lindl. and Cattleya guttata Lindl., drought alters the leaf water balance, increases CAM expression, and may consequently increase the FWU rate. To test this hypothesis, drought stress was induced by restricting water for 30 days in C. forbesii (n = 4) and C. guttata (n = 6). After that, we evaluated the relative water content (RWC), overnight organic acid accumulation (ΔH⁺), and FWU capacity. To verify the pathway of water entry into the leaves, we used the apoplastic marker Lucifer Yellow. We observed that in both Cattleya species, drought impacted leaf water status, photosynthetic activity, and FWU capacity. After water restriction, the leaf water status decreased, impairing photosynthetic activity in both species (lower RWC, potential quantum yield of PSII, and quantum efficiency under light-adapted conditions), whereas nonphotochemical quenching increased. Despite minor changes in ΔH⁺ values, FWU increased following drought, partially supporting our hypothesis. In both Cattleya species, LY solution diffused through the epidermis and reached the mesophyll; however, in C. guttata, this occurred only through the abaxial epidermis. These results demonstrate that FWU may serve as a strategy for Cattleya species to cope with water scarcity intensified by drought periods.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71047"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148875488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Responses of Invasive and Native Plant Species to Combined Cd and Microplastic Pollution.","authors":"Rasheed Akbar, Yanwen Bo, Cheng Jin, Tariq Aslam, Kashif Ahmad, Zhiyuan Liu, Jianfan Sun, Jibiao Fan","doi":"10.1111/ppl.71089","DOIUrl":"10.1111/ppl.71089","url":null,"abstract":"<p><p>The co-occurrence of heavy metal contamination and biodegradable microplastic (polylactic acid, PLA) pollution poses increasing risks to terrestrial plant communities and soil functioning, yet species-specific responses to combined stress remain poorly understood. Cd and microplastics frequently co-occur in agricultural soils, where microplastics can alter cadmium mobility, bioavailability, and transport pathways, potentially modifying metal toxicity and plant stress responses compared with single-pollutant exposure. We investigated the responses of the invasive Bidens pilosa and the native Solanum nigrum grown in monoculture and mixed culture under combined cadmium (Cd) and biodegradable microplastic (PLA) stress by integrating plant growth, photosynthetic performance, oxidative physiology, and rhizosphere biochemical processes. Combined Cd-MP exposure markedly reduced plant growth, chlorophyll content (SPAD), photosystem II efficiency (Fv/Fm), nitrogen accumulation, biomass production, and rhizosphere enzyme activities associated with carbon, nitrogen, and phosphorus cycling. However, B. pilosa maintained greater physiological stability under stress, characterized by higher antioxidant enzyme activities (SOD, CAT, POD), lower reactive oxygen species (H₂O₂, O₂˙^-) accumulation, and reduced lipid peroxidation (MDA), whereas S. nigrum exhibited stronger oxidative damage and functional impairment. Multivariate analyses further revealed that root antioxidant capacity was closely associated with rhizosphere microbial enzyme activity, suggesting a root-centered regulatory mechanism linking plant stress tolerance to soil functioning. Overall, the invasive species showed greater tolerance to combined contamination and maintained relatively higher rhizosphere functional activity than the native species, indicating that multi-pollutant stress may alter competitive interactions between invasive and native plants in contaminated environments.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71089"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148888048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural Etiolation in Spearmint (Mentha spicata L. var. crispa) Shoots Emerging From Rhizomes-Plastids, Glandular Hairs, and Phytochemical Profile.","authors":"Bernadett Szögi-Tatár, Adrienn Dobi, Andrea Böszörményi, Enkhjin Enkhbileg, Nóra Gampe, Imre Boldizsár, Anna Skribanek, Katalin Solymosi","doi":"10.1111/ppl.71109","DOIUrl":"10.1111/ppl.71109","url":null,"abstract":"<p><p>Spearmint (Mentha spicata L. var. crispa) is a widely cultivated aromatic herb valued both as a culinary ingredient and as a source of bioactive compounds, such as essential oils and phenolics, which contribute to its traditional uses and potential medicinal applications. In this work, we provided the first evidence of natural etiolation with etioplast and prolamellar body formation in leaves and stems of spearmint shoots emerging in the soil from underground rhizomes. Soil-grown and hydroponically dark-forced shoots exhibited comparable etiolation symptoms, plastid ultrastructure, and chlorophyll biosynthesis as revealed by 77 K fluorescence emission spectroscopy, establishing laboratory etiolated plants as reliable models for natural skotomorphogenetic processes. Comparison of 14-day-old light-grown and dark-forced shoots developing from the rhizomes of hydroponically cultured adult plants revealed that the slightly smaller etiolated leaves had significantly smaller peltate glandular hairs than light-grown ones (diameter: 54-56 μm vs. 59 μm, respectively), but their number and density were not influenced by light and they were always present in much higher numbers on the abaxial side than on the adaxial side of the leaf surfaces. Principal component analysis of the volatile organic compound and phenolic profiles distinctly separated light-grown and dark-forced shoots, with fewer compounds being present and in lower relative abundance in etiolated tissues, and more pronounced differences observed in the phenolic composition. These data underscore the value of etiolation and greening research, provide new models for studying development and environmental interactions under naturally light-deprived conditions, and offer novel insights into primary and secondary metabolism in hidden spearmint organs.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71109"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148977459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carotenoids in Stress Tolerance: Current Understanding and Future Prospects.","authors":"Shriya Sharma, Rajagopal Subramanyam","doi":"10.1111/ppl.71095","DOIUrl":"10.1111/ppl.71095","url":null,"abstract":"<p><p>Carotenoids are ubiquitous isoprenoid metabolites whose biological significance extends far beyond their classical roles as antioxidants and light-harvesting pigments. Across the tree of life, these molecules exhibit remarkable structural and functional diversity, enabling organisms to fine-tune photoprotection, redox homeostasis, membrane organization, and stress-responsive signaling. In this review, we provide a conceptual synthesis of carotenoid diversity and function across organisms, with emphasis on how carotenoid composition and accumulation are dynamically regulated under environmental and biotic stresses. We highlight stress-induced carotenoid plasticity, manifested as selective accumulation, rerouting of pathways, and the production of signaling apocarotenoids as a central adaptive strategy across photosynthetic organisms. Finally, we discuss emerging evidence and competing hypotheses regarding the mechanistic diversity of non-photochemical quenching and the evolving yet debated role of carotenoids in energy-dependent quenching. Together, this review emphasizes carotenoids as dynamic metabolic regulators that integrate many facets of photosynthetic performance, stress responses and signaling.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71095"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13549744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148906879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Histone Methyltransferase OsSET37 Positively Regulates Rice Seed Vigor.","authors":"Xinpeng Xi, Simin Ma, Lian Yu, Yanfen Liu, Qing He, Hanyang Cai, Yuan Qin, Liping Liu","doi":"10.1111/ppl.71105","DOIUrl":"10.1111/ppl.71105","url":null,"abstract":"<p><p>Seed vigor is a critical determinant of crop establishment and yield, yet the epigenetic mechanisms underlying this trait remain largely unexplored. Here, we identified a histone methyltransferase, OsSET37, and characterized its essential role in regulating seed vigor in rice (Oryza sativa). Consistent with OsSET37 being a member of the SDG (SET domain group) family, our results demonstrate that the loss of OsSET37 leads to a marked decrease in H3K36me3 in vivo. Phenotypic analysis showed that osset37 mutants displayed normal vegetative and reproductive growth, but exhibited delayed germination and enhanced sensitivity to salt, drought, and ABA stresses during seed germination relative to the wild type. Y2H (Yeast two-hybrid) assays demonstrated that OsSET37 interacts with stress-responsive proteins OsSUB1C and OsDIP1. Furthermore, transcriptome analysis and qRT-PCR indicated that OsSET37 modulates the expression of key genes involved in the GA (gibberellin) and ABA (abscisic acid) signaling pathways. Collectively, our findings suggest that OsSET37 positively regulates seed vigor by modulating H3K36me3 deposition and fine-tuning the hormone signaling network, highlighting its potential as a valuable target for rice breeding programs aimed at improving stress resilience.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71105"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13553160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148913104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"A Maize Calcineurin B-Like Interacting Protein Kinase ZmCIPK42 Confers Salt Stress Tolerance\".","authors":"","doi":"10.1111/ppl.71086","DOIUrl":"https://doi.org/10.1111/ppl.71086","url":null,"abstract":"","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71086"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149017776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Non-Photochemical Loss in PSII in High- and Low-Light-Grown Leaves of Vicia faba Quantified by Several Fluorescence Parameters Including L_NP, F₀/F'_m, a Novel Parameter\".","authors":"","doi":"10.1111/ppl.71118","DOIUrl":"https://doi.org/10.1111/ppl.71118","url":null,"abstract":"","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"178 5","pages":"e71118"},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149112164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}