Physiologia plantarum最新文献_第5页

Flash drought as possible contributor to seedling dieback in the endangered conifer Abies koreana. 突发性干旱可能是濒危针叶树韩国冷杉幼苗枯死的原因。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70218

Umashankar Chandrasekaran, Minsu Lee, Jiwon Baek, Yunhee Park, Ah Reum Han, Neung-Hwan Oh, Hyeyeong Choe, Hyun Seok Kim

{"title":"Flash drought as possible contributor to seedling dieback in the endangered conifer Abies koreana.","authors":"Umashankar Chandrasekaran, Minsu Lee, Jiwon Baek, Yunhee Park, Ah Reum Han, Neung-Hwan Oh, Hyeyeong Choe, Hyun Seok Kim","doi":"10.1111/ppl.70218","DOIUrl":"https://doi.org/10.1111/ppl.70218","url":null,"abstract":"Tree species grown at high altitudes experience significantly greater stress than those at lower altitudes. A notable example is Abies koreana, a conifer recently classified as endangered due to a decline in normal seedling distribution within Korean natural forests. While several hypotheses have been proposed to explain this phenomenon, the underlying causes remain unclear. Recent studies highlight that Korean forest tree species are increasingly vulnerable to flash drought (FD) events. However, it is still unknown whether this intense FD event affects the growth and distribution of high-altitude grown and endangered species like Abies koreana. To address this gap, we investigated the effects of FD on root carbon allocation, volatile biosynthesis, fatty acid modulation, and genome-wide modifications. Exposure to FD in three-year-old A. koreana seedlings primarily disrupted leaf chlorophyll biosynthesis, likely due to the depletion of root water and non-structural carbohydrates (NSC) transport to above-ground parts. Additionally, FD caused severe morphological changes, including reductions in root collar diameter along with root cortical senescence. These alterations are linked to transcriptomic variations, particularly mRNA decay and the repression of genes coding for ribosomal proteins. Seedlings exposed to FD also exhibited increased levels of abscisic acid (ABA) and poly-unsaturated fatty acids. The observed patterns and molecular mechanisms in FD-treated seedlings differed significantly from those observed for control and mild drought (MD) treatments. These findings suggest that FD conditions trigger rapid carbon reserve depletion and gene repression associated with root structural integrity, potentially leading to seedling mortality in Abies koreana seedlings.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70218"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant proximity reduces seed yield in Arabidopsis plants by decreasing the number of ovule primordia. 植物接近通过减少胚珠原基的数量来降低拟南芥的种子产量。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70220

Irma Roig-Villanova, Salvador Torres-Montilla, Estefanía López-Ortiz, Maurizio Di Marzo, Ángela Sánchez-García, Anna Esteve-Codina, Aurelio Gómez-Cadenas, Jaume F Martínez-García

{"title":"Plant proximity reduces seed yield in Arabidopsis plants by decreasing the number of ovule primordia.","authors":"Irma Roig-Villanova, Salvador Torres-Montilla, Estefanía López-Ortiz, Maurizio Di Marzo, Ángela Sánchez-García, Anna Esteve-Codina, Aurelio Gómez-Cadenas, Jaume F Martínez-García","doi":"10.1111/ppl.70220","DOIUrl":"https://doi.org/10.1111/ppl.70220","url":null,"abstract":"Proximity of vegetation, which is influenced by planting density, significantly impacts plant development. In Arabidopsis thaliana, it is well established that simulated shade, which mimics the proximity of other plants, triggers hypocotyl and petiole elongation, accelerates flowering and suppresses axillary bud growth. Although there is evidence that simulated shade affects reproduction beyond accelerating flowering, its impact on the development of reproductive tissues after plant architecture establishment (i.e., once flowering has begun) remains poorly explored. Here, we report that simulated shade promotes silique and pedicel elongation while reducing seed production, primarily by decreasing ovule number formation. Shade perception triggers rapid changes in gene expression in reproductive tissues, with some genes showing tissue-specific responses and others being induced in both seedlings and reproductive tissues, highlighting a conserved core of shade-responsive genes associated with light perception, photosynthesis and hormone regulation. However, while shade-induced elongation responses occur rapidly, reduction in ovule number requires prolonged shade exposure, suggesting distinct regulatory pathways for these responses. These findings shed light on the complex interplay between common (e.g., elongation and core gene expression) and tissue-specific responses (e.g., ovule formation and specialized gene expression) to shade, contributing to the developmental plasticity of Arabidopsis. Furthermore, they enhance our understanding of how external signals, indicative of vegetation proximity, can modulate seed production, a genetically determined process.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70220"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of nanobodies in Arabidopsis thaliana strengthens the absorption capacity of triclosan from growth media. 纳米体在拟南芥中的表达增强了生长介质对三氯生的吸收能力。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70163

Weixia Liu, Mengya Li, Xiangyu Zhang, Yongqiang Ma, Qing X Li, Christophe Morisseau, Bruce D Hammock, Ting Xu

{"title":"Expression of nanobodies in Arabidopsis thaliana strengthens the absorption capacity of triclosan from growth media.","authors":"Weixia Liu, Mengya Li, Xiangyu Zhang, Yongqiang Ma, Qing X Li, Christophe Morisseau, Bruce D Hammock, Ting Xu","doi":"10.1111/ppl.70163","DOIUrl":"10.1111/ppl.70163","url":null,"abstract":"Triclosan (TCS) is a broad-spectrum antimicrobial disinfectant widely used in pharmaceuticals and personal care products (PPCPs). Due to the extensive usage of PPCPs, TCS inevitably entered the environment and pose harmful effects on the ecosystem. Phytoremediation is an attractive approach to remove TCS from the environment. In this study, a gene encoding the anti-TCS nanobody was transformed into Arabidopsis thaliana (A. thaliana) to enhance the absorption capacity of TCS. Nanobodies are small antibody fragments (ca. 15 kDa) derived from the variable domain of camelid heavy-chain-only antibodies. We constructed two transgenic lines, the T-S-C line with nanobody expression throughout the plant and the T-S-P line with nanobody expression dominant in the roots, were constructed. The expression of nanobodies in A. thaliana alleviated the phytotoxicity of TCS. T-S-C and T-S-P exhibited significantly stronger tolerance to TCS toxicity than the wild type (WT), in either a solid medium system or a hydroponics system. Under the stress of TCS, the seedlings of both transgenic plants exhibited an increase in root length and fresh weight compared to those of WT. Moreover, in the presence of TCS, the activities of superoxide dismutase, peroxidase, catalase, and glutathione in transgenic plants were higher than those in WT. The concentration of TCS absorbed into the T-S-C and T-S-P plants from the solid medium increased by 50.0% and 24.1%, and from the hydroponics system increased by 55.6% and 38.0%, respectively, compared to those absorbed by WT. This study provides a proof of principle that transforming nanobodies into plants represents a novel technology to improve the efficiency of phytoremediation for environmental pollutants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70163"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UV-B orchestration of growth, yield and grain quality traits highlights modifications of source-to-sink relationship in pearl millet cultivars. UV-B对珍珠粟品种生长、产量和品质性状的调控，凸显了源库关系的改变。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70141

Pooja Singh, Krishna Kumar Choudhary

{"title":"UV-B orchestration of growth, yield and grain quality traits highlights modifications of source-to-sink relationship in pearl millet cultivars.","authors":"Pooja Singh, Krishna Kumar Choudhary","doi":"10.1111/ppl.70141","DOIUrl":"10.1111/ppl.70141","url":null,"abstract":"Climate change and stratospheric ozone layer dynamics have altered the intensity of ultraviolet B (UV-B) radiation, affecting the growth, yield, and metabolic responses of major cereal crops. As a result, to meet the future demand scenario for growing population and health concerns, millets have been recognized as important substitutes. Among them, pearl millet has shown resilience against various abiotic stresses, but its response to UV-B radiation has not yet been explored. Recognizing its importance in present global food systems, the present investigation aimed to analyse the effect of elevated UV-B (eUV-B; ambient+7.2 kJm-2d-1) on four cultivars (HHB-272, HHB-67, MPMH-21, and MPMH-17) of pearl millet during panicle development stage and grain filling stage under natural field conditions. The results indicated that UV-B stress altered growth morphology (plant height, number of leaves, leaf area, and panicle length) at both stages, with less pronounced effects on cultivars HHB-272 and HHB-67. Declined growth indices [relative growth rate (RGR), absolute growth rate (AGR), net assimilation rate (NAR), and leaf area ratio (LAR)] at panicle stage revealed predominance of UV-B stress. Grain yield was positively affected in all the cultivars, indicating better resource allocation to different important needs, thereby altering the trade-offs between growth and development as reported in our present study. The grain quality (total soluble sugars, reducing sugars, total free amino acids, starch content, soluble protein) of harvested seeds exhibited a decreased quality response index, and metabolic pathway analysis of the metabolites identified through UHPLC-HRMS indicated a shift in photoassimilates towards fatty acid biosynthesis. These findings help to understand various plant metabolic pathways, potentially revealing the resilience mechanism involved in pearl millet under eUV-B exposure.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70141"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive analytical method 'Regulatome' revealed a novel pathway for aerenchyma formation under waterlogging in wheat. 一种综合分析方法“调节组”揭示了小麦涝渍条件下通气组织形成的新途径。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70157

Hao Gao, Mingjiong Chen, Nanfei Jin, Lingzhen Ye, Guoping Zhang, Qiufang Shen, Zhengyuan Xu

{"title":"A comprehensive analytical method 'Regulatome' revealed a novel pathway for aerenchyma formation under waterlogging in wheat.","authors":"Hao Gao, Mingjiong Chen, Nanfei Jin, Lingzhen Ye, Guoping Zhang, Qiufang Shen, Zhengyuan Xu","doi":"10.1111/ppl.70157","DOIUrl":"10.1111/ppl.70157","url":null,"abstract":"Waterlogging is a major abiotic stress restricting crop yield globally, and aerenchyma formation is one of the most important adaptive strategies in waterlogging-tolerant plants. However, the conservation of this process remains poorly understood, and additional pathways are yet to be identified. Here, physiological, anatomical, transcriptomic, and metabolomic analyses were conducted on wheat seedlings under normal and waterlogging conditions. Waterlogging caused growth inhibition and physiological damage, as well as induced aerenchyma formation in roots. A total of 10,346 differentially expressed genes and 3,419 differential metabolites were identified in roots. In addition to the AP2/ERF (APETALA2/ETHYLENE RESPONSIVE FACTOR) gene family, integrating analyses also revealed the role of LOB/AS2 (LATERAL ORGAN BOUNDARIES/ASYMMETRIC LEAVES2) in aerenchyma formation under waterlogging. It was revealed that the classical pathway of aerenchyma formation mediated by ethylene response, as well as synergy of calcium ion and reactive oxygen species, was deeply conserved in both monocots and eudicots during 160 million years of evolution through gene co-expression networks of cross-species. The newly introduced concept 'Regulatome' supported the classical pathway of aerenchyma formation, with a proposed model of the jasmonic acid signalling pathway involved in waterlogging, suggesting its usefulness in gene identification and function exploration. These findings provide a novel insight into the regulatory mechanisms of aerenchyma formation and breeding approaches for developing wheat cultivars with high waterlogging tolerance.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70157"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Rootstock's Cotyledon-Regulated Fructokinase ClFRK1 Contributes to Graft Union Formation in Watermelon. 砧木子叶调节果糖激酶ClFRK1对西瓜嫁接结合的影响

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70144

Akebaierjiang Kadeer, Mu Xiong, Chen-le Hou, Bei-Ping Zheng, Hong-Liang Zhang, Xiang-Shuai Wu, Yehia Abouseif, Zu-Hua Zheng, Zhi-Long Bie, Michitaka Notaguchi, Yuan Huang

{"title":"The Rootstock's Cotyledon-Regulated Fructokinase ClFRK1 Contributes to Graft Union Formation in Watermelon.","authors":"Akebaierjiang Kadeer, Mu Xiong, Chen-le Hou, Bei-Ping Zheng, Hong-Liang Zhang, Xiang-Shuai Wu, Yehia Abouseif, Zu-Hua Zheng, Zhi-Long Bie, Michitaka Notaguchi, Yuan Huang","doi":"10.1111/ppl.70144","DOIUrl":"10.1111/ppl.70144","url":null,"abstract":"Grafting is a traditional horticultural practice that enhances plant resilience against biotic and abiotic stresses. However, the influence of specific tissues, such as rootstock cotyledons, on graft union formation is not well understood. This study investigates the impact of rootstock cotyledon removal on graft healing in watermelon and its underlying mechanisms. Our results indicate that grafting with rootstock cotyledons (+C) consistently resulted in higher survival rates and better growth outcomes compared to grafting without rootstock cotyledons (-C). This effect was more pronounced in cultivated watermelon rootstocks, which have lower hypocotyl sugar content than wild watermelon rootstocks. Transcriptomic analysis revealed that cotyledon removal disrupted sugar metabolism and affected gene expression related to cell division and tissue development. A fructokinase, ClFRK1, was identified among the candidate genes positively correlated with graft survival rate and healing degree. Silencing ClFRK1 reduced callus proliferation, delayed graft healing and reduced survival rate. Conversely, fructose treatment increased ClFRK1 expression levels at the graft junction, which promoted callus proliferation and vascular reconnection. We propose a novel regulatory model for how ClFRK1 regulates graft union formation. These findings underscore new insights into the interactions and synergistic processes between the graft interface and non-grafted organs during graft union formation and also enrich our understanding of fructokinase.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70144"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silicon modulates nitrogen and secondary metabolism in Glycyrrhiza uralensis under drought and salt stress conditions. 干旱和盐胁迫下硅对甘草氮和次生代谢的调节作用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70138

Guohui Chen, Xiaojia Zhang, Gaochang Cui, Wenjin Zhang, Qiuxian Bai, Xinhui Zhang

{"title":"Silicon modulates nitrogen and secondary metabolism in Glycyrrhiza uralensis under drought and salt stress conditions.","authors":"Guohui Chen, Xiaojia Zhang, Gaochang Cui, Wenjin Zhang, Qiuxian Bai, Xinhui Zhang","doi":"10.1111/ppl.70138","DOIUrl":"10.1111/ppl.70138","url":null,"abstract":"Glycyrrhiza uralensis Fisch (G. uralensis) is a key species for windbreak and sand fixation, possessing notable pharmacological and economic value. However, the yield of G. uralensis is considerably impacted due to its cultivation in arid, semi-arid, and salt-affected regions. Silicon (Si) has been reported to improve plant tolerance to drought and salt stress by regulating nitrogen and secondary metabolism. Herein, the effects of Si treatment on nitrogen and secondary metabolism of G. uralensis seedlings under drought (D), salt (S), and drought-salt (SD) stresses were investigated in combination with physiological and transcriptomic analyses. The results indicated that stress conditions significantly inhibited the growth of G. uralensis seedlings by suppressing nitrogen and secondary metabolism. Si treatment counteracted these inhibitions to some extent. Specifically, Si treatment increased soluble protein content by approximately 15% by regulating the nitrogen metabolism of G. uralensis under D stress. Furthermore, Si treatment elevated the content of glycyrrhetinic acid by about 89% under SD stress by increasing the content of primary metabolites and regulating the expression of enzymes involved in the biosynthesis of glycyrrhizic acid and liquiritin, including 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), squalene synthase (SQS), and β-amyrin synthase (β-AS). In summary, our findings suggest that Si could alleviate the adverse effects induced by drought and/or salt stresses on the growth of G. uralensis seedlings by regulating nitrogen metabolisms, which further triggered the accumulation of secondary metabolites, ultimately improving the stress resistance of cultivated G. uralensis seedlings. This work provides direction for Si to improve stress resistance.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70138"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MATE transporter DTX44 is involved in regulating flowering time in Arabidopsis. MATE转运体DTX44参与拟南芥开花时间的调控。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70238

Penghong Zhang, Fang Yu, Xiaoxian Zhu, Jiangzhe Zhao, Kewei Zhang

{"title":"MATE transporter DTX44 is involved in regulating flowering time in Arabidopsis.","authors":"Penghong Zhang, Fang Yu, Xiaoxian Zhu, Jiangzhe Zhao, Kewei Zhang","doi":"10.1111/ppl.70238","DOIUrl":"https://doi.org/10.1111/ppl.70238","url":null,"abstract":"The precise timing of flowering is crucial for the transition from vegetative to reproductive phases and is regulated by both endogenous and exogenous signals. Here, we demonstrate that DTX44, a member of the multidrug and toxic compound extrusion (MATE) transporter family, plays a role in facilitating flowering in Arabidopsis thaliana. Overexpressing of DTX44 resulted in early flowering, while a dtx44 mutant exhibited delayed flowering. The early-flowering phenotype induced by DTX44 overexpression was repressed by the ft (FLOWERING LOCUS T) mutant. In contrast, the delayed flowering phenotype of dtx44 mutants was suppressed by the flc (FLOWERING LOCUS C) mutant. Subcellular localization analysis of GFP-DTX44 in the Arabidopsis protoplasts and Nicotiana benthamiana leaves suggests that DTX44 localizes to the endoplasmic reticulum. GUS staining of the DTX44pro:DTX44-GUS transgenic plants indicated that the DTX44 gene is widely expressed in the shoots and roots. The grafting experiments between the dtx44 mutant or DTX44 overexpression lines and wild type suggested that DTX44 expression in the shoots and roots contribute to flowering time. RNA-seq analysis of dtx44 mutant and wild-type seedlings suggests that DTX44 is involved in the biosynthesis or signaling of multiple hormones in response to abiotic stresses. In summary, our study reveals the biological function of the MATE transporter DTX44 in Arabidopsis flowering. The substrate of DTX44 remains to be elucidated in future studies.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70238"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid polymorphism of plant thylakoid membranes. The dynamic exchange model - facts and hypotheses. 植物类囊体膜的脂质多态性。动态交换模型——事实与假设。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70230

Győző Garab, Kinga Böde, Ondřej Dlouhý, Zoltán Násztor, Václav Karlický, András Dér, Vladimír Špunda

{"title":"Lipid polymorphism of plant thylakoid membranes. The dynamic exchange model - facts and hypotheses.","authors":"Győző Garab, Kinga Böde, Ondřej Dlouhý, Zoltán Násztor, Václav Karlický, András Dér, Vladimír Špunda","doi":"10.1111/ppl.70230","DOIUrl":"https://doi.org/10.1111/ppl.70230","url":null,"abstract":"The light reactions of oxygenic photosynthesis are performed by protein complexes embedded in the lipid bilayer of thylakoid membranes (TMs). Bilayers provide optimal conditions for the build-up of the proton motive force (pmf) and ATP synthesis. However, functional plant TMs, besides the bilayer, contain an inverted hexagonal (HII) phase and isotropic phases, a lipid polymorphism due to their major, non-bilayer lipid species, monogalactosyldiacylglycerol (MGDG). The lipid phase behavior of TMs is explained within the framework of the Dynamic Exchange Model (DEM), an extension of the fluid-mosaic model. DEM portrays the bilayer phase as inclusions between photosynthetic supercomplexes - characterized by compromised membrane impermeability and restricted sizes inflicted by the segregation propensity of lipid molecules, safe-guarding the high protein density of TMs. Isotropic phases mediate membrane fusions and are associated with the lumenal lipocalin-like enzyme, violaxanthin de-epoxidase. Stromal-side proteins surrounded by lipids give rise to the HII phase. These features instigate experimentally testable hypotheses: (i) non-bilayer phases mediate functional sub-compartmentalization of plant chloroplasts - a quasi-autonomous energization and ATP synthesis of each granum-stroma TM assembly; and (ii) the generation and utilization of pmf depend on hydrated protein networks and proton-conducting pathways along membrane surfaces - rather than on strict impermeability of the bilayer.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70230"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12008737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulatory role of ABA-responsive element binding factors in plant abiotic stress response. aba响应元件结合因子在植物非生物胁迫响应中的调控作用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70233

Dandan Zha, Yuxi He, Jianghua Song

引用次数: 0