Physiologia plantarum最新文献_第5页

Deletion of Flv3A facilitates long-term H₂ photoproduction in diazotrophic Anabaena sp. PCC 7120.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70087

Meilin He, Anita Santana-Sánchez, Gábor Szilveszter Tóth, Maria Ermakova, Darius Collard, Sergey Kosourov, Yagut Allahverdiyeva

{"title":"Deletion of Flv3A facilitates long-term H2 photoproduction in diazotrophic Anabaena sp. PCC 7120.","authors":"Meilin He, Anita Santana-Sánchez, Gábor Szilveszter Tóth, Maria Ermakova, Darius Collard, Sergey Kosourov, Yagut Allahverdiyeva","doi":"10.1111/ppl.70087","DOIUrl":"https://doi.org/10.1111/ppl.70087","url":null,"abstract":"Molecular hydrogen (H2) is a promising energy carrier, and its production by photosynthetic microorganisms holds substantial potential for advancing renewable energy generation. The nitrogenase-mediated H2 production using heterocyst-forming cyanobacteria represents a promising approach, as the process utilizes light energy and photosynthetic reductants while being naturally protected from O2-rich environments by its restriction to microoxic heterocyst cells. We investigated the impact of deleting the vegetative cell-specific flavodiiron protein, Flv3A, on the long-term H2 photoproduction of the model heterocyst-forming cyanobacterium Anabaena sp. PCC 7120. The H2 photoproduction response was evaluated under varying atmospheric conditions, with or without N2 and O2, and compared to the ∆hupL mutant, which is deficient in the large subunit of uptake hydrogenase, and the ∆hupL/flv3A double mutant. Unlike the ΔhupL mutant, H2 photoproduction in Δflv3A is not enhanced by increased nitrogenase activity or high accumulation of sugars in cells. Our results suggest that the absence of the vegetative cell-localized Flv3A positively affects H2 photoproduction in heterocysts by simultaneously downregulating hupL expression and enhancing the O2 tolerance of nitrogenase via a yet unexplored mechanism. These findings advance our understanding of nitrogenase-driven H2 production and provide a new strategy to address key limitations in long-term photobiological H2 production.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70087"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-function relation of cytokinins determines their differential efficiency in mediating tobacco resistance against Pseudomonas syringae. 细胞分裂素的结构-功能关系决定了它们介导烟草对丁香假单胞菌抗性的差异效率。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70028

Dominik K Großkinsky, Eva M Molin, Federico Bosetto, Kerstin Edelsbrunner, Michal Oravec, Kristýna Večeřová, Jan Tříska, Thomas Roitsch

{"title":"Structure-function relation of cytokinins determines their differential efficiency in mediating tobacco resistance against Pseudomonas syringae.","authors":"Dominik K Großkinsky, Eva M Molin, Federico Bosetto, Kerstin Edelsbrunner, Michal Oravec, Kristýna Večeřová, Jan Tříska, Thomas Roitsch","doi":"10.1111/ppl.70028","DOIUrl":"10.1111/ppl.70028","url":null,"abstract":"The classic plant growth-promoting phytohormone cytokinin has been identified and established as a mediator of pathogen resistance in different plant species. However, the resistance effect of structurally different cytokinins appears to vary and may regulate diverse mechanisms to establish resistance. Hence, we comparatively analysed the impact of six different adenine- and phenylurea-type cytokinins on the well-established pathosystem Nicotiana tabacum-Pseudomonas syringae. The efficiency of resistance effects was evaluated based on impacts on the host plant defence response by scoring infection symptoms and the direct impact on the pathogen by assessment of proliferation in planta. To identify common and cytokinin-specific components involved in resistance effects, transcriptome profiling and targeted metabolomics were conducted in leaves treated with the different cytokinins. We observed clearly different potentials of the tested cytokinins in either suppressing infection symptoms or pathogen proliferation. Gene regulation and metabolite analyses revealed cytokinin-type specific impacts on defence components, such as salicylic acid and related signalling, expression of PR proteins, and regulation of specialised metabolism. Cytokinins also strongly affected plant cell physiological parameters, such as a remarkable decrease in amino acid pools. Hence, this study provides comparative information on the efficiency of diverse cytokinins in mediating resistance in one well-studied pathosystem and insights into the specific regulation of resistance effects mediated by different cytokinin molecules. This is particularly relevant for studies on the function of cytokinins or other phytohormones and compounds interacting with cytokinin activities in the context of pathogen infections and other stress scenarios, considering the diverse cytokinins present in plants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70028"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving sulforaphane content in broccoli sprouts by applying Se: transcriptome profiling and coexpression network analysis provide insights into the mechanistic response. 利用Se转录组分析和共表达网络分析提高西兰花芽中萝卜硫素的含量，为其机制响应提供了新的见解。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70037

Shuxiang Mao, Junwei Wang, Zhijun Guo, Huiping Huang, Shengze Wang, Dandan Fei, Juan Liu, Qi Wu, Jin Nie, Qiuyun Wu, Ke Huang

{"title":"Improving sulforaphane content in broccoli sprouts by applying Se: transcriptome profiling and coexpression network analysis provide insights into the mechanistic response.","authors":"Shuxiang Mao, Junwei Wang, Zhijun Guo, Huiping Huang, Shengze Wang, Dandan Fei, Juan Liu, Qi Wu, Jin Nie, Qiuyun Wu, Ke Huang","doi":"10.1111/ppl.70037","DOIUrl":"https://doi.org/10.1111/ppl.70037","url":null,"abstract":"Sulforaphane (SF) is a sulfur (S)-containing isothiocyanate found in cruciferous vegetables and is known for its potent anticancer properties. Broccoli sprouts, in particular, are considered safe and healthy dietary choices due to their high SF content and other beneficial biological activities, such as enhanced metabolite ingestion. The application of selenium (Se) is an excellent approach to enhance the abundance of SF. Previous studies have often focused on gene expression and changes in the synthetic substrates of glucoraphanin (RAA) to explain SF variation in response to Se application. However, the regulatory network and other physiological and biochemical reactions involved in the regulation of SF biosynthesis are poorly understood. In this study, Se-treated broccoli sprouts had higher SF and RAA contents; they increased with increasing Se application. Using RNA-seq in combination with KEGG, GO, phenotypic, and WGCNA analyses, it was observed that not only gene expression was induced but also that glutathione serves as an S donor for SF biosynthesis and acts as an oxidative stress reliever as a result of Se treatment. Additionally, a module related to glucosinolate biosynthesis was identified. Yeast one-hybrid system and dual luciferase reporter assay were utilized. These assays demonstrated the hub transcription factors GATA22, ERF12-like, and MYB108 would directly bind to SUR1 promoter and positively regulate its expression. Our study presents the first global overview of the role of GSH metabolism in response to Se for SF biosynthesis, and provides a novel and valuable gene resource for the molecular breeding of high-SF broccoli.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70037"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in drought avoidance rather than differences in the fast versus slow growth spectrum explain distributions of two Asclepias species. 在干旱避免的差异，而不是在快与慢生长谱的差异解释两种Asclepias分布。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70034

Steven L Matzner, Emily R Konz, Samantha A Marts, Haley M Eversman, Kyla M Kasuske, Trinity L Atkins, Sneha Acharya, Lara C Matuck, Lillian M Derynck, Sydney Kreutzmann, Avery G Selberg, Kelli M Glisar, Sydney A Capers, Victoria L Lind, Sarah Olimb, Carrie F Olson-Manning

{"title":"Differences in drought avoidance rather than differences in the fast versus slow growth spectrum explain distributions of two Asclepias species.","authors":"Steven L Matzner, Emily R Konz, Samantha A Marts, Haley M Eversman, Kyla M Kasuske, Trinity L Atkins, Sneha Acharya, Lara C Matuck, Lillian M Derynck, Sydney Kreutzmann, Avery G Selberg, Kelli M Glisar, Sydney A Capers, Victoria L Lind, Sarah Olimb, Carrie F Olson-Manning","doi":"10.1111/ppl.70034","DOIUrl":"10.1111/ppl.70034","url":null,"abstract":"Understanding factors that determine a species' geographical range is crucial for predicting climate-induced range shifts. Two milkweed species, Asclepias syriaca and Asclepias speciosa, have overlapping ranges along a moisture gradient in North America and are primary food sources for endangered monarch caterpillars. With decreasing moisture, long-lived species often exhibit slower growth and greater drought tolerance, while many annual species exhibit faster growth strategies. Using this fast-slow framework, we assessed whether traits of these two sister species differ along a fast-slow growth continuum and could explain their distributions. We measured leaf and root functional traits in common gardens and greenhouse experiments. In key measures indicative of drought tolerance (e.g., growth, transpiration, and water potentials), the species were nearly identical. Contrary to expectations, A. speciosa did not exhibit greater drought tolerance, raising the question of how it survives in the more arid west. A reciprocal transplant study showed selection against A. syriaca in the western garden and that A. speciosa was better able to avoid seedling mortality. Focusing on seedling establishment, we found that A. speciosa exhibited faster deep-root development and a narrow leaf phenotype associated with slower wilting and delayed drought-induced mortality. Rather than differences on the fast-slow growth spectrum, our results indicate that A. speciosa avoids drought through faster deep-root growth and a slower wilting phenotype. Our study suggests that A. syriaca's range is limited by its drought tolerance, while A. speciosa employs a number of drought avoidance strategies to survive in more arid environments.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70034"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11800957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A combination of salt stress and waterlogging provides protection to tomato plants against the negative effects of waterlogging individually applied.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70116

Taufika Islam Anee, Rido Ramadano Rachman, Zhao Ziqi, Nobuhiro Suzuki

{"title":"A combination of salt stress and waterlogging provides protection to tomato plants against the negative effects of waterlogging individually applied.","authors":"Taufika Islam Anee, Rido Ramadano Rachman, Zhao Ziqi, Nobuhiro Suzuki","doi":"10.1111/ppl.70116","DOIUrl":"https://doi.org/10.1111/ppl.70116","url":null,"abstract":"Salt stress and waterlogging are two of the most common abiotic stresses in nature, often occurring concurrently. However, our understanding of the mechanisms underlying responses of plants to a combination of these stresses remains limited. In this study, we investigated growth, physiological and biochemical responses of Solanum lycopersicum cv. Micro-Tom to salt stress, waterlogging and the combination of both. Under waterlogging individually, plants showed increased plant height and longer root length. However, they exhibited a significantly smaller leaf area, fewer leaves, reduced fresh and dry weights, and lower relative water content compared to plants grown under controlled conditions. These effects were more severe than those caused by salt stress alone. Interestingly, the growth inhibition from waterlogging was alleviated under combined salt and waterlogging stress. This attenuation may be associated with decreased accumulation of H₂O₂ and oxidized lipids, along with increased proline and photosynthetic pigment contents compared with waterlogging individually applied. However, ROS accumulations and contents of photosynthetic pigments were not straightforwardly linked to the activity of photosynthesis. In addition, activities of various antioxidant enzymes such as CAT, GPX and GST as well as those involved in the AsA-GSH cycle were differently altered by salt stress and waterlogging, individually and in combination. Taken together, these results suggest that the response of tomato plants to salt stress and waterlogging, individually and in combination, can be differently modulated via fine-tuning of acclimation mechanisms to oxidative stress.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70116"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal changes associated with arbuscular mycorrhizal fungi indicate defense-like alterations in virus-stressed grapevine.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70136

Emanuel Gaši, Matevž Likar, Vicent Arbona, Miguel González-Guzmán, Katarina Hančević, Raffaella Balestrini, Mate Čarija, Marjana Regvar, Giorgio Gambino, Fabiano Sillo, Tomislav Radić

{"title":"Hormonal changes associated with arbuscular mycorrhizal fungi indicate defense-like alterations in virus-stressed grapevine.","authors":"Emanuel Gaši, Matevž Likar, Vicent Arbona, Miguel González-Guzmán, Katarina Hančević, Raffaella Balestrini, Mate Čarija, Marjana Regvar, Giorgio Gambino, Fabiano Sillo, Tomislav Radić","doi":"10.1111/ppl.70136","DOIUrl":"https://doi.org/10.1111/ppl.70136","url":null,"abstract":"Grapevine is an economically important crop, affected by major production losses due to high virus prevalence. Arbuscular mycorrhizal fungi (AMF) can reduce the impact of plant biotic stresses. However, hormonal response to the simultaneous presence of viruses and AMF remains largely unknown. In this study, we explored the potential of AMF to modify the grapevine's defense response to compatible virus infections. We used GRSPaV, GLRaV-3, and GPGV as infectious viral agents, separately or in different combinations. Two AMF inoculums were tested for their bioprotective abilities, RHIZ (Rhizophagus irregularis) and MIX (R. irregularis, Funneliformis mosseae, F. caledonium). Generally, MIX induced stronger physiological responses than RHIZ inoculum, especially during the earlier phase of symbiosis. The main findings were connected to the hormonal profile of the grapevine infected by all three viruses and inoculated with MIX. In particular, salicylic acid (SA) and abscisic acid (ABA) concentrations were induced five and fifteen months post AMF inoculation, respectively. Expressions of VvNCED1 and VvBG1 were up-regulated in uninoculated grapevines, indicating slower induction of stress response mechanisms. Parameters related to plant vigour and growth were induced in grapevine at both time points, regardless of the virus combination. In conclusion, the defense-like response induced by AMF in grapevines infected with multiple viruses is characterized by the induction of ABA and SA, accompanied by a consistent enhancement of vigor parameters. This study confirms AMF symbiosis as a potentially promising additional tool for combating viral diseases in vineyards.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70136"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lanthanum supplementation with abscisic acid modulates rhizosphere dynamics through changes in nitric oxide synthesis in wheat.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70126

Moksh Mahajan, Rudra Narayan Sahoo, M Iqbal R Khan

{"title":"Lanthanum supplementation with abscisic acid modulates rhizosphere dynamics through changes in nitric oxide synthesis in wheat.","authors":"Moksh Mahajan, Rudra Narayan Sahoo, M Iqbal R Khan","doi":"10.1111/ppl.70126","DOIUrl":"https://doi.org/10.1111/ppl.70126","url":null,"abstract":"Lanthanum (La), a rare earth element (REE) found its wide applications in the agricultural land practices. Utilization of La as a fertilizer is based on its concentration for staple crops, including wheat (Triticum aestivum). We have investigated the role of a beneficial dose of La (60 μM) along with ABA (10 μM) in improving wheat root dynamics and defense systems (ascorbate-glutathione pathway, secondary metabolites, and nitric oxide (NO) biosynthesis). Co-application of La60 and ABA significantly enhanced all the root dynamic attributes along with defense systems and reduced production of reative oxygen species (ROS). The use of the NO inhibitor c-PTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxy-3-oxide], proved that the impact of co-application of La60 and ABA on root growth dynamics was mediated by NO biosynthesis. Thus, this study could work as the base to understand the dose-dependent behavior of La in plants, offering valuable implications for enhancing crop resilience, optimizing agricultural practices, and mitigating the detrimental effects of La toxicity on wheat root development. This research gives a comprehensive insight into root dynamics influenced by ABA with La dose optimisations in wheat.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70126"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotyping and metabolomics insights into the effect of melatonin in lettuce under non-stress and salinity conditions. 非胁迫和盐度条件下褪黑激素对莴苣影响的表型和代谢组学研究。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70055

Elena Secomandi, Biancamaria Senizza, Marco Armando De Gregorio, Begona Miras-Moreno, Rosa Maria Rivero, Pascual Garcia-Perez, Luigi Lucini

{"title":"Phenotyping and metabolomics insights into the effect of melatonin in lettuce under non-stress and salinity conditions.","authors":"Elena Secomandi, Biancamaria Senizza, Marco Armando De Gregorio, Begona Miras-Moreno, Rosa Maria Rivero, Pascual Garcia-Perez, Luigi Lucini","doi":"10.1111/ppl.70055","DOIUrl":"10.1111/ppl.70055","url":null,"abstract":"Melatonin (MLT) is an indole derivative that exhibits hormone-like activities in plants, regulating multiple aspects of growth and development. Due to its role in mitigating oxidative stress and facilitating osmoprotectant accumulation, MLT enhances abiotic stress tolerance, although the pathways and metabolic mechanisms involved remain unclear despite being studied in various crops. This work aimed to investigate the changes elicited by the exogenous MLT application at different concentrations (10, 50, 150 μM) and its role in mitigating the salinity stress in Lactuca sativa L. through metabolomics and phenotyping approaches. Our results clearly indicated that MLT increases photosynthetic efficiency at high dosage (150 μM) at either early or late salinity stress conditions (p < 0.01). Untargeted metabolomics provided insight into the significant effect of salinity and MLT (p < 0.01 in both cases, according to multivariate chemometrics), mediated by a broad reprogramming involving secondary metabolism, phytohormones, fatty acids and amino acids biosynthesis. In detail, 150 μM MLT induced an adjustment of the phytohormones profile to reduce the salinity-induced damages. Our findings support the well-known potential of melatonin in alleviating salinity stress. These findings address existing challenges in studying the molecular effects of MLT in mitigating abiotic stress, providing insights into the biochemical pathways that drive its effectiveness. In this sense, further research is acknowledged to provide a multidisciplinary high throughput perspective leading to its exploitation in a wide range of crops of agricultural and economic importance.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70055"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11733844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of syringic acid in enhancing growth, photosynthesis, and antioxidant defense in lettuce exposed to arsenic stress. 丁香酸对砷胁迫下生菜生长、光合作用和抗氧化防御的促进作用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70051

Melike Balci, Busra Arikan-Abdulveli, Evren Yildiztugay, Ceyda Ozfidan-Konakci

{"title":"Role of syringic acid in enhancing growth, photosynthesis, and antioxidant defense in lettuce exposed to arsenic stress.","authors":"Melike Balci, Busra Arikan-Abdulveli, Evren Yildiztugay, Ceyda Ozfidan-Konakci","doi":"10.1111/ppl.70051","DOIUrl":"10.1111/ppl.70051","url":null,"abstract":"Heavy metal pollution, especially arsenic toxicity, significantly impairs plant growth and development. Phenolic acids, known for their antioxidant properties and involvement in stress signaling, are gaining increased attention as plant secondary metabolites with the potential to enhance plant resistance to these stressors. This study aimed to investigate the effects of different concentrations of syringic acid (SA1, 10 μM; SA2, 250 μM; SA3, 500 μM) on growth, photosynthetic parameters, and antioxidant activity in lettuce seedlings subjected to arsenic stress (As, 100 μM). Arsenic stress reduced growth by 56.7%, water content by 7.39%, and osmotic potential by 26.2% in lettuce leaves compared to control. Conversely, SA1 and SA2 treatments mitigated the adverse effects of arsenic on growth and preserved the water balance in plants. However, the SA3 treatment led to a decrease in growth by 18.9% and 39.5% in the SA3 and As+SA3 groups, respectively, indicating that high-dose SA treatment adversely affected lettuce leaves under both control and stress conditions. Exogenous SA1 treatment significantly improved photosynthesis, whereas SA2 provided milder benefits and SA3 did not reduce the adverse effects of arsenic exposure. Arsenic stress increased H2O2 content by 47.3% and lipid peroxidation by 33.4% in lettuce seedlings. SA1 treatment effectively reduced oxidative stress by enhancing the activities of key antioxidant enzymes, such as superoxide dismutase (SOD) and peroxidase (POX). Moreover, SA1 was successful in maintaining the glutathione (GSH) pool, whereas SA2 primarily promoted ascorbate (AsA) regeneration. In conclusion, 10 μM of syringic acid (SA1) was identified as the optimal dose for reducing arsenic stress in lettuce by enhancing antioxidant activity and supporting growth. Overall, the findings underscore the potential of SA1 treatment in enhancing the resilience of lettuce to heavy metal toxicity.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70051"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of temperature and light on photoprotection in the model diatom Phaeodactylum tricornutum. 温度和光照对模型硅藻 Phaeodactylum tricornutum 光保护的协同效应。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70039

Chiara E Giossi, Dila B Bitnel, Marie A Wünsch, Peter G Kroth, Bernard Lepetit

{"title":"Synergistic effects of temperature and light on photoprotection in the model diatom Phaeodactylum tricornutum.","authors":"Chiara E Giossi, Dila B Bitnel, Marie A Wünsch, Peter G Kroth, Bernard Lepetit","doi":"10.1111/ppl.70039","DOIUrl":"10.1111/ppl.70039","url":null,"abstract":"Diatoms dominate phytoplankton communities in turbulent waters, where light fluctuations can be frequent and intense. Due to this complex environment, these heterokont microalgae display remarkable photoprotection strategies, including a fast Non-Photochemical Quenching (NPQ). However, in nature, several abiotic parameters (such as temperature) can influence the response of photosynthetic organisms to light stress in a synergistic or antagonistic manner. Yet, the combined effects of light and these other drivers on the photosynthetic and photoprotective capacity of diatoms are still poorly understood. In this work, we investigated the impact of short-term temperature and light stress on the model diatom Phaeodactylum tricornutum, combining NPQ induction-recovery assays or light curves with a broad gradient of superimposed temperature treatments (5 to 35°C). We employed mutant lines deficient in NPQ generation (vde KO) or recovery (zep3 KO) and wild type. We found that temperature and light have a synergistic effect: lower temperatures limited both the photosynthetic capacity and NPQ, while the general photophysiological performance was enhanced with warming, up to a heat-stress limit (above 30°C). We discuss the temperature effects on NPQ induction and recovery and propose that these are independent from the energy requirements of the cells and result from altered xanthophyll cycle dynamics. Namely, we found that de-epoxidation activity strongly increases with temperature, outweighing epoxidation and resulting in a positive increase of NPQ with temperature. Finally, we propose that in a short-term time frame, temperature and light have a synergistic and not antagonistic effect, with a positive relationship between increasing temperature and NPQ.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70039"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11733657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0