Physiologia plantarum最新文献_第5页

Metabolomic approaches suggest two mechanisms of drought response post-anthesis in Mediterranean oat (Avena sativa L.) cultivars. 代谢组学方法提示地中海燕麦（Avena sativa L.）品种花后干旱响应的两种机制。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70181

Aiswarya Girija, Francisco J Canales, Bahareh Sadat Haddadi, Rachel Dye, Fiona Corke, Kevin Williams, Helen Phillips, Manfred Beckmann, Elena Prats, John H Doonan, Luis A J Mur

{"title":"Metabolomic approaches suggest two mechanisms of drought response post-anthesis in Mediterranean oat (Avena sativa L.) cultivars.","authors":"Aiswarya Girija, Francisco J Canales, Bahareh Sadat Haddadi, Rachel Dye, Fiona Corke, Kevin Williams, Helen Phillips, Manfred Beckmann, Elena Prats, John H Doonan, Luis A J Mur","doi":"10.1111/ppl.70181","DOIUrl":"10.1111/ppl.70181","url":null,"abstract":"Oats (Avena sativa L) is a temperate cereal and an important healthy cereal cultivated for food and feed. Therefore, understanding drought responses in oats could significantly impact oat production under harsh climatic conditions. In particular, drought during anthesis (flowering) affects grain filling, quality and yield. Here, we characterised metabolite responses of two Mediterranean oat (Avena sativa L.) cultivars, Flega and Patones, during drought stress at anthesis. In the more drought-tolerant Patones, the developing grains from the top (older) and bottom (younger) spikelets of primary panicle were found to be larger in size in response to drought, suggesting accelerated grain development. Flega showed a more rapid transition to flowering and grain development under drought. The metabolomes of source (sheath, flag leaf, rachis) and sink (developing grains) tissues from Patones showed differential accumulation in fatty acids levels, including α-linolenic acid, sugars and amino acids with drought. Flega showed enhanced energy metabolism in both source and sink tissues. Lower levels of glutathione in source tissues and the accumulation of ophthalmic acid in the grains of Flega were indicators of oxidative stress. Our study revealed two distinct metabolite regulatory patterns in these cultivars during drought at anthesis. In Patones, α-linolenic acid-associated processes may accelerate grain-filling, while in Flega oxidative stress appears to influence traits such as flowering time. Overall, this work provides a first insight into the metabolite regulation in oat's source and sink tissues during anthesis under drought stress.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70181"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11949858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling ecophysiological and molecular adjustments in the photosynthesis-respiration balance during Fusarium graminearum infection in wheat spikes. 小麦穗部镰刀菌侵染过程中光合作用-呼吸平衡的生理生态和分子调节。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70150

Florian Rocher, Pierre Bancal, Alain Fortineau, Géraldine Philippe, Philippe Label, Thierry Langin, Ludovic Bonhomme

{"title":"Unravelling ecophysiological and molecular adjustments in the photosynthesis-respiration balance during Fusarium graminearum infection in wheat spikes.","authors":"Florian Rocher, Pierre Bancal, Alain Fortineau, Géraldine Philippe, Philippe Label, Thierry Langin, Ludovic Bonhomme","doi":"10.1111/ppl.70150","DOIUrl":"10.1111/ppl.70150","url":null,"abstract":"Wheat responses to F. graminearum result in a deep and sharp reprogramming of a wide range of biological processes, including energy-associated functions and related metabolisms. Although these impacts have been thoroughly described at the molecular scale through proteomics and transcriptomics studies, phenotypic studies are still needed to fill the gap between the observed molecular events and the actual impacts of the disease on the ecophysiological processes. Taking advantage of the gas exchange method, the effects of two F. graminearum strains of contrasting aggressiveness on spike's photosynthesis and respiration-associated processes during an early infection time course were deeply characterized. Besides, an RNAseq-based expression profiling of the genes involved in the photosynthesis, respiration and stomatal movement processes was also performed when plants were challenged using the same two fungal strains. In response to Fusarium head blight, CO2 assimilation and CO2 diffusion adjustments matched transcriptomic data, showing altered photosynthetic processes and sharp gene regulations unrelated to symptom development. In contrast, although ecophysiological characterization clearly demonstrated respiration adjustments along with the F. graminearum's infection process, the gene regulations involved were not fully captured transcriptionally. We demonstrated that combining gas exchange methods with transcriptomics is especially effective in enhancing and deepening our understanding of complex physiological adjustments, providing unique and complementary insights that cannot be predicted from a single approach.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70150"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of pepper bZIP transcription factor CaADBZ1 in abscisic acid signalling and drought stress response. 辣椒bZIP转录因子CaADBZ1在脱落酸信号和干旱胁迫响应中的作用

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70159

Jihye Choi, Chae Woo Lim, Sung Chul Lee

{"title":"Role of pepper bZIP transcription factor CaADBZ1 in abscisic acid signalling and drought stress response.","authors":"Jihye Choi, Chae Woo Lim, Sung Chul Lee","doi":"10.1111/ppl.70159","DOIUrl":"10.1111/ppl.70159","url":null,"abstract":"In plants, basic-region/leucine-zipper (bZIP) transcription factors are key regulators of stress responses mediated by various phytohormone signalling pathways. However, the roles of bZIP transcription factors in pepper, particularly those associated with ABA signalling and drought stress, remain poorly understood. In this study, we isolated the CaADBZ1 (Capsicum annuum ABA and Dehydration-Induced bZIP transcription factor 1) gene, a member of the group A family, and analysed its functions in response to dehydration stress and ABA signalling. The expression of CaADBZ1 was specifically induced by dehydration and exogenous ABA treatment, not salinity and osmotic stress. CaADBZ1 was found to have transactivation activity in yeast cells, which was dependent on the N-terminal of CaADBZ1 (amino acids 1-112), harbouring a highly conserved C1 domain. Notably, a dual-luciferase reporter assay revealed that CaADBZ1 modulated the expression of CaOSR1, a dehydration stress-responsive gene in pepper plants. Functional studies in both pepper and Arabidopsis plants revealed that the modulation of CaADBZ1 expression level affected dehydration stress resistance in pepper and Arabidopsis plants. CaADBZ1-silenced pepper Arabidopsis plants showed dehydration stress-sensitive phenotypes characterized by higher transpiration rates and reduced expression of dehydration-responsive genes compared to control plants. Conversely, overexpression of the CaADBZ1 gene in Arabidopsis plants enhanced dehydration stress resistance. Moreover, CaADBZ1-overexpressing Arabidopsis transgenic plants showed increased ABA sensitivity during the seedling stage. Collectively, our findings suggest that CaADBZ1 plays a crucial role in enhancing dehydration stress tolerance in plants by positively regulating ABA sensitivity and dehydration-responsive gene expression.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70159"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SYNTAXIN OF PLANTS 81 regulates fatty acid desaturation by mediating Acyl Carrier Protein Desaturase 5 during seed development in Arabidopsis thaliana. SYNTAXIN OF PLANTS 81通过介导酰基载体蛋白去饱和酶5调控拟南芥种子发育过程中的脂肪酸去饱和。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70173

Xiaonan Zhao, Hailong Zhang, Ronghui Pan, Guochen Qin, Jie Dong, Yang Zhu, Mingjing Wang, Hongyu Zhou, Mengjie Gong, Pengcheng Wang, Lixin Li, Lixi Jiang

{"title":"SYNTAXIN OF PLANTS 81 regulates fatty acid desaturation by mediating Acyl Carrier Protein Desaturase 5 during seed development in Arabidopsis thaliana.","authors":"Xiaonan Zhao, Hailong Zhang, Ronghui Pan, Guochen Qin, Jie Dong, Yang Zhu, Mingjing Wang, Hongyu Zhou, Mengjie Gong, Pengcheng Wang, Lixin Li, Lixi Jiang","doi":"10.1111/ppl.70173","DOIUrl":"https://doi.org/10.1111/ppl.70173","url":null,"abstract":"Lipids are essential for building cells and are used as important seed reserves. Fatty acids (FAs) are the key structural units of lipids, forming their hydrophobic tails in triglycerides and phospholipids. FA synthesis starts in plastids and is completed in the endoplasmic reticulum (ER). SYNTAXIN OF PLANTS 81 (SYP81), a Qa-soluble N-ETHYLMALEIMIDE SENSITIVE FACTOR attachment protein receptor (Qa-SNARE), regulates vesicle trafficking between the ER and Golgi apparatus, yet its role in FA synthesis is unknown. Here, we examined the expression of SYP81 during Arabidopsis thaliana seed development and found that the syp81 mutation reduced Acyl-Acyl Desaturase 5 (AAD5) accumulation in plastids of Arabidopsis embryonic cells and thus significantly decreased unsaturated FA production. Pull-down experiments identified possible interactors with SYP81, including various Stearoyl-Acyl carrier protein Desaturases (SADs), notably AAD5, and Translocons at Outer envelope membranes of Chloroplast (TOCs), especially TOC33. To validate these interactions, CoIP, yeast-two-hybridization assays, and bimolecular-fluorescence-complementation experiments were performed. The results of these experiments supported the interaction between SYP81 and AAD5, as well as SYP81 and TOC33. Based on these findings, a model was proposed, suggesting that pre-AAD5, recruited by SYP81, translocates from the ER to the plastids through the TIC-TOC complex mediations. Within the plastids, pre-AAD5 then matures into its catalytically active form, enabling subsequent FA desaturation.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70173"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seaweed extract combined with boron promotes the growth of sugar beet by improving the photosynthetic performance under boron deficiency. 海藻浸提液与硼配合施用可改善缺硼条件下甜菜的光合性能，促进甜菜生长。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70195

Songlin Yang, Wenyu Wu, Shangxuan Liu, Xiaoyu Zhao, Muhammad Riaz, Muhammad Ishfaq, Yan Wang, Abudukadier Kuerban, Wang Xing, Baiquan Song

{"title":"Seaweed extract combined with boron promotes the growth of sugar beet by improving the photosynthetic performance under boron deficiency.","authors":"Songlin Yang, Wenyu Wu, Shangxuan Liu, Xiaoyu Zhao, Muhammad Riaz, Muhammad Ishfaq, Yan Wang, Abudukadier Kuerban, Wang Xing, Baiquan Song","doi":"10.1111/ppl.70195","DOIUrl":"10.1111/ppl.70195","url":null,"abstract":"Biostimulants can improve mineral nutrient effectiveness, but the physiological mechanisms by which seaweed extract, a natural biostimulant, and boron (B) fertilizer promote the growth of sugar beet under B deficiency are not clear. In this study, B and seaweed extract were applied under B-deficiency (0.32 mg B kg-1 soil) and potentially B-deficient conditions (0.69 mg B kg-1 soil), and the growth and photochemical properties of sugar beet seedlings were investigated. The results indicated that B application alone or with seaweed extract promoted sugar beet growth, with the combined application having a significantly enhanced effect. When comparing the two soils with different B content (deficient and moderately deficient), the spray of seaweed extract under the B-deficient condition was more effective. After the addition of seaweed extract, the B content of the shoots and roots increased by 28.56% and 12.64%, respectively, under B deficiency(0.32 mg B kg-1 soil). Furthermore, the content of chlorophyll a (Chla), chlorophyll b (Chlb), and the net photosynthesis rate (Pn) increased by 8.96%, 30.57%, and 13.74%, respectively. The addition of seaweed extract significantly improved the light saturation point (Pm), with a 35.00% increase compared to the control. Additionally, it increased the quantum yield (ETO/CSm) of leaf electron transfer per unit area and reduced the absorption of light energy loss of the PS reaction center (DIO/RC) and per unit area (DIO/CSm), thereby improving photosynthetic performance and significantly increasing aboveground dry matter accumulation by 19.05%. In conclusion, seaweed extract can enhance B absorption, light energy capture and utilization ability. It provides the theoretical evidence for the regulation of B nutrition in sugar beet and the rational application of biostimulants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70195"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome and metabolome analysis revealed that phenylpropanoid and flavonoid biosynthesis respond to drought in tiger nut. 转录组学和代谢组学分析揭示了虎坚果苯丙素和类黄酮的生物合成对干旱的响应。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70191

Zhang Qi, Yan Cheng, Yuling Gao, Runqing Liu, Haoxin Li, Jinqi Yu, Jiaxuan Guo, Meiqing Li, Caihua Li, Yuhuan Li, Hongda Wang, Qingqing Xu, Jiaxi Liu, Xuewei Sun, Zhongsheng Mu, Jidao Du

{"title":"Transcriptome and metabolome analysis revealed that phenylpropanoid and flavonoid biosynthesis respond to drought in tiger nut.","authors":"Zhang Qi, Yan Cheng, Yuling Gao, Runqing Liu, Haoxin Li, Jinqi Yu, Jiaxuan Guo, Meiqing Li, Caihua Li, Yuhuan Li, Hongda Wang, Qingqing Xu, Jiaxi Liu, Xuewei Sun, Zhongsheng Mu, Jidao Du","doi":"10.1111/ppl.70191","DOIUrl":"10.1111/ppl.70191","url":null,"abstract":"Tiger nuts (Cyperus esculentus) have emerged as a novel oil crop, being utilized as raw materials for obtaining industrial ink. Drought is a serious stress that significantly affects the entire plant and reduces its yield. The seedling stage is crucial as it determines the future growth and yield. Consequently, it is essential to enhance the ability of tiger nuts to mitigate drought at the seedling stage. A comprehensive analysis was conducted on roots and leaves, including their phenotypes, physiological indicators, transcriptomes, and metabolomes. The results revealed that leaves and roots were affected by drought stress, as evidenced by phenotypic data such as leaf area and physiological indicators, including changes in peroxidase and catalase activity, malondialdehyde content, electrolyte leakage, and superoxide anion levels. Drought imposed greater effects on leaves. Phenylpropanoid and flavonoid biosynthesis were identified as candidate pathways using transcriptome and metabolome analysis, Real-Time Quantitative PCR (RT-qPCR), and physiological verifications. However, the response modes of the root and leaf parts differed based on the enriched pathways analysis, indicating that the changes in the content of some metabolites were contrasting between the roots and leaves. The study revealed the molecular mechanisms under drought, particularly the synergistic responses in leaves and roots, providing insights and a theoretical basis for enhancing the drought tolerance of tiger nuts.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70191"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Azolla mediated alterations in grain yield and quality in Rice. 杜鹃花介导水稻产量和品质的变化。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70158

Nadia Bazihizina, Chiara Paleni, Stefania Caparrotta, Tania Macchiavelli, Giorgia Guardigli, Ilaria Colzi, Michele Petrillo, Cristina Gonnelli, Antonietta Saccomanno, Veronica Gregis, Stefano Mancuso, Diego Comparini, Martin M Kater, Camilla Pandolfi

{"title":"Azolla mediated alterations in grain yield and quality in Rice.","authors":"Nadia Bazihizina, Chiara Paleni, Stefania Caparrotta, Tania Macchiavelli, Giorgia Guardigli, Ilaria Colzi, Michele Petrillo, Cristina Gonnelli, Antonietta Saccomanno, Veronica Gregis, Stefano Mancuso, Diego Comparini, Martin M Kater, Camilla Pandolfi","doi":"10.1111/ppl.70158","DOIUrl":"10.1111/ppl.70158","url":null,"abstract":"Rice is one of the most important cereal crops worldwide. To boost its production in a sustainable manner, co-cultivation with Azolla species is often used to supplement its nitrogen (N) demands. However, beyond N nutrition, the physiological and developmental effects of azolla on rice remain unclear. This study investigates these mechanisms by analysing growth, inflorescence meristem transcriptomics, yield, and grain ionomics in rice plants grown alone (R) or with azolla (R + A) in non-limiting N conditions. During the vegetative stage, the presence of azolla increased allocation of resources to rice shoots without affecting root growth, while in the reproductive stage, it improved panicle architecture, with a 6% increase in length and up to 26% increase in panicle branching. Nevertheless, while this increase in panicle branching in R + A translated into a greater number of grains per plant, grain weight declined. As a result, yields were similar between R and R + A. There was also an azolla-induced increment in several mineral elements in R + A grains, with the notable exception of zinc, which declined by more than 30%. Finally, the presence of azolla altered the expression of several gene families, and in particular, it led to the upregulation of numerous transcription factors from the AP2/ERF, WRKY and NAM families. Interestingly, the presence of azolla also led to the upregulation of several genes (including WRKY transcription factors) involved in resistance to several pathogens and abiotic stresses. Overall, our results suggest that rice-azolla co-cultivation has implications that go beyond N-nutrition for sustainable intensification of rice production.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70158"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11947517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143721113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High hydraulic safety, water use efficiency and a conservative resource-use strategy in woody species of high-altitude environments: A global study. 高海拔环境木本植物的水力安全、水资源利用效率和资源利用策略

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70064

Muhammad Waseem, Yakov Kuzyakov, Marc Carriquí, Christine Scoffoni, Kaiping Zhang, Md Mahadi Hasan, Guang-Qian Yao, Lei He, Jing Shao, Fengyuan Mei, Ting-Shuai Shi, Xiang-Wen Fang

{"title":"High hydraulic safety, water use efficiency and a conservative resource-use strategy in woody species of high-altitude environments: A global study.","authors":"Muhammad Waseem, Yakov Kuzyakov, Marc Carriquí, Christine Scoffoni, Kaiping Zhang, Md Mahadi Hasan, Guang-Qian Yao, Lei He, Jing Shao, Fengyuan Mei, Ting-Shuai Shi, Xiang-Wen Fang","doi":"10.1111/ppl.70064","DOIUrl":"10.1111/ppl.70064","url":null,"abstract":"Understanding the impact of altitude on leaf hydraulic, gas exchange, and economic traits is crucial for comprehending vegetation properties and ecosystem functioning. This knowledge also helps to elucidate species' functional strategies regarding their vulnerability or resilience to global change effects in alpine environments. Here, we conducted a global study of dataset encompassing leaf hydraulic, gas exchange, and economic traits for 3391 woody species. The results showed that high-altitude species possessed greater hydraulic safety (Kleaf P50), higher water use efficiency (WUEi) and conservative resource use strategy such as higher leaf mass per area, longer leaf lifespan, lower area-based leaf nitrogen and phosphorus contents, and lower rates of photosynthesis and dark respiration. Conversely, species at lower altitudes exhibited lower hydraulic safety (Kleaf P50), lower water use efficiency (WUEi) and an acquisitive resource use strategy. These global patterns of leaf traits in relation to altitude reveal the strategies that alpine plants employ for hydraulic safety, water use efficiency, and resource, which have important implications for predicting forest productivity and acclimation to rapid climate change.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70064"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of CPKs in ethylene-induced Arabidopsis stomatal closure and their crosstalk with H₂O₂ and NO signalling. CPKs在乙烯诱导拟南芥气孔关闭中的作用及其与H2O2和NO信号的串扰

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70196

Xue Li, Lixiao Wang, Meixiang Zhang, Junmin He, Yuyan An

{"title":"Roles of CPKs in ethylene-induced Arabidopsis stomatal closure and their crosstalk with H2O2 and NO signalling.","authors":"Xue Li, Lixiao Wang, Meixiang Zhang, Junmin He, Yuyan An","doi":"10.1111/ppl.70196","DOIUrl":"https://doi.org/10.1111/ppl.70196","url":null,"abstract":"Calcium-dependent protein kinases (CPKs) play crucial roles in plant guard cell signal transduction. Ethylene is known to induce stomatal closure, with the hydrogen peroxide (H2O2)-nitric oxide (NO) signalling module being pivotal to this process. However, the specific roles of CPKs in this process and their interactions with H2O2 and NO remain unclear. In this study, we screened Arabidopsis mutants of nine CPKs and found that in the loss-of-function mutants for CPK3, CPK4, CPK6, CPK11, CPK21, and CPK33, exogenous ethylene failed to induce stomatal closure, indicating that these CPKs act as positive regulators in ethylene-induced stomatal closure. Mutants' stomatal responses to H2O2 and NO treatment and changes of endogenous H2O2 and NO levels in guard cells upon ethylene treatment indicated that CPK3, CPK4, CPK11, and CPK33 function upstream of the H2O2-NO module, while CPK6 and CPK21 act downstream. Furthermore, NADPH oxidases play critical roles in ethylene-induced H2O2 production. We identified the interactions of CPK3, CPK4, and CPK11 with AtRBOHF, and CPK4 and CPK11 with AtRBOHD using four different assays, and exogenous ethylene enhanced these interactions. These results suggest that CPK3, CPK4, and CPK11 may mediate ethylene-induced H2O2 formation in guard cells through their interactions with AtRBOHD/F. Additionally, exogenous ethylene significantly upregulates the expression of CPK3, CPK4, CPK6, CPK11 and CPK21, providing a potential mechanism by which ethylene modulates CPKs. Our findings not only establish the role of CPKs in ethylene guard cell signalling but also offer insights into the mechanism by which ethylene activates NADPH oxidases to initiate H2O2 production.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70196"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green light induces Solanum lycopersicum JA synthesis and inhibits Botrytis cinerea infection cushion formation to resist grey mould disease. 绿光诱导茄JA合成，抑制灰霉菌感染垫形成，抵抗灰霉菌病。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70156

Yunfei Cai, Jiali Ying, Youju Ye, Shuangshuang Wen, Renjuan Qian

引用次数: 0