Physiologia plantarum最新文献

{"title":"Moderate Temperature Reduction Changes the High-Light Acclimation Strategy of Lettuce Plants.","authors":"Tapio Lempiäinen, Dorota Muth-Pawlak, Julia P Vainonen, Eevi Rintamäki, Mikko Tikkanen, Eva-Mari Aro","doi":"10.1111/ppl.70298","DOIUrl":"10.1111/ppl.70298","url":null,"abstract":"<p><p>In nature, environmental conditions are constantly changing, requiring plants to have numerous regulatory mechanisms to keep light harvesting and metabolism in balance. Here, we show that high light (HL) induces a much stronger non-photochemical quenching (NPQ) when lettuce plants are exposed to 1500 μmol photons m^-2 s^-1 for 4 h at 13°C (low temperature, LT) compared to 23°C (growth temperature, GT). GT/HL treatment induced NPQ to relax during 1 h in darkness. In contrast, LT/HL treatment induced an exceptionally high NPQ that only partially relaxed during 1 h in darkness at GT. Such a high sustained NPQ (sNPQ) cannot be explained by the canonical NPQ mechanism(s). Instead, sNPQ was associated with a transient increase in phosphorylation of minor LHCII antenna proteins, LHCB4.1/LHCB4.2 and partial disassembly of PSII-LHCII complexes. This coincided with increased expression of the light-harvesting-like proteins SEP2 and ELIP1.2, the PSII assembly proteins HCF173 and LPA3, and accumulation of the pre-D1 protein, indicating delayed PSII repair. These results lead us to propose that under LT/HL, the phosphorylation of LHCB4.1/LHCB4.2 initiates the disassembly of PSII-LHCII supercomplexes, allowing accumulated SEP2 to bind to CP47, presumably leading to quenching of the inner PSII core antenna. The free CP43 core antenna, released from PSII at an early stage of repair, is proposed to be protected by accumulated LPA3. Apparently, the cascades of regulatory mechanisms are specific to each combination of environmental changes, depending on their concomitant effects on chloroplast redox balance and PSII repair rate, with induced PSII core antenna quenching contributing to sNPQ.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70298"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Analysis of Faba Bean AP2/ERF Genes Suggests Potential Roles of VfAP2-1 and VfERF-99 in Abiotic and Biotic Stress Responses.","authors":"Limeng Dong, Ting Huang, Shuo Han, Xiaowen Han, Junliang Yin, Lu Hou, Yujiao Liu","doi":"10.1111/ppl.70356","DOIUrl":"10.1111/ppl.70356","url":null,"abstract":"<p><p>The AP2/ERFs not only participate in regulating signal networks, but they also play important roles in the process of plant growth and stress response. However, systematic research of AP2/ERF in Vicia faba is lacking. In this study, VfAP2/ERF was systematically identified and their characteristics were comprehensively analyzed. In total, 145 VfAP2/ERFs were identified, which were unevenly distributed across six chromosomes, and according to phylogenetic relationships, VfAP2/ERFs could be classified into five subgroups. Cis-elements analysis showed that VfAP2/ERF promoters harbored numerous elements functionally relating to light response, plant hormone, abiotic stress response, and plant growth and development response. Expression profiling analysis indicated that VfAP2/ERFs were broadly expressed during growth and development, and were responsive to drought and salt stresses. RT-qPCR revealed that six VfAP2/ERF genes were upregulated under drought and salt stress. Inoculation assay showed that VfAP2-1 and VfERF-99 could enhance resistance to pathogens. Further research shows that VfAP2-1 and VfERF-99 positively influence ROS homeostasis, resulting in the accumulation of H₂O₂ and O₂ ^- under abiotic and biotic stresses, which inhibited the colonization of pathogens. Additionally, VfAP2-1 and VfERF-99 could significantly increase the content of chlorophyll a, carotenoids, and total chlorophyll, suggesting their possible roles in promoting photosynthesis. This study comprehensively analyzed VfAP2/ERFs and preliminarily explored the function of VfAP2-1 and VfERF-99 in biotic/abiotic stresses and photosynthesis, which laid the foundation for deciphering their functional mechanisms.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70356"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global Tissue Specific Transcriptome Analysis of Musa spp., cv. Grand Naine (AAA) Across Twelve Different Tissues.","authors":"Marimuthu Kumaravel, Ganesan Karthika, Patel Prashanti, Adi Doron-Faigenboim, Amir Raz, Navot Galpaz","doi":"10.1111/ppl.70339","DOIUrl":"10.1111/ppl.70339","url":null,"abstract":"<p><p>Untargeted trait manipulation through molecular breeding in a sterile and polyploid crop such as banana can introduce adverse pleiotropic effects. This limitation can be overcome with a precise targeted approach using tissue-specific promoters to induce the desired gene in specific tissues. As the banana plant is highly susceptible to many biotic and abiotic stresses, its defense traits must be enhanced. To generate resilient bananas that can withstand various stresses, we aimed to identify tissue-specific and differentially expressed genes through an RNA-sequencing approach. Transcriptomic analysis was carried out in 12 different developmental tissues (primary root, secondary root, corm rhizome, tip meristem, leaf sheath pseudostem, leaf blade, leaf midrib, peduncle, unripe fruit peel, unripe fruit pulp, ripe fruit peel and ripe fruit pulp) of Cavendish type banana-cv. Grand Naine (AAA). Results showed that 8098 genes were differentially expressed among the 12 tissues, 146 genes were uniquely expressed in specific tissues, and 48 genes were constitutively expressed in all tissues. Functional annotations exhibited that most of the differentially expressed genes were involved in the oxidation-reduction process (391), followed by transcriptional regulation (185) and phosphorylation (184). Gene-enrichment analysis showed that many differentially expressed genes were involved in defense mechanisms associated with phenylpropanoid (55) and terpenoid (49) pathways. The promoters of identified differentially expressed genes can be studied for transient expression with reporter genes and then in stable transformation studies, thereby avoiding pleiotropic effects in genome-manipulated bananas.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70339"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Structural Diversity and Evolution of the D1 Subunit of Photosystem II Using AlphaFold and Foldtree.","authors":"Tom Dongmin Kim, Daniella Pretorius, James W Murray, Tanai Cardona","doi":"10.1111/ppl.70284","DOIUrl":"10.1111/ppl.70284","url":null,"abstract":"<p><p>Although our knowledge of photosystem II has expanded to include time-resolved atomic details, the diversity of experimental structures of the enzyme remains limited. Recent advances in protein structure prediction with AlphaFold offer a promising approach to fill this gap in structural diversity in non-model systems. This study used AlphaFold to predict the structures of the D1 protein, the core subunit of photosystem II, across a broad range of photosynthetic organisms. The prediction produced high-confidence structures, and structural alignment analyses highlighted conserved regions across the different D1 groups, which were in line with high pLDDT scoring regions. In contrast, varying pLDDT in the DE loop and terminal regions appears to correlate with different degrees of structural flexibility or disorder. Subsequent structural phylogenetic analysis using Foldtree provided a tree that is in good agreement with previous sequence-based studies. Moreover, the phylogeny supports a parsimonious scenario in which far-red D1 and D1^INT evolved from an ancestral form of G4 D1. This work demonstrates the potential of AlphaFold and Foldtree to study the molecular evolution of photosynthesis.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70284"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought Stress Modifies the Source-Sink Dynamics of Nitrogen-Fixing Soybean Plants Prioritizing Roots and Nodules.","authors":"María Isabel Rubia, Estíbaliz Larrainzar, Cesar Arrese-Igor","doi":"10.1111/ppl.70276","DOIUrl":"10.1111/ppl.70276","url":null,"abstract":"<p><p>Soybean plants are one of the most cultivated legume crops worldwide. Their ability to establish nitrogen-fixing symbiosis with rhizobium bacteria allows the reduction of molecular nitrogen to ammonium, contributing to a reduction in the dependence on nitrogen fertilizers. However, nitrogen fixation is highly sensitive to environmental stresses, such as water deficit, and the regulatory mechanisms underlying this inhibition remain debatable. In the current study, we analyzed carbon (C) allocation dynamics in drought-stressed soybean plants following the application of [U-¹³C]-sucrose to source leaves. Three sets of plants were analyzed: well-watered plants, mild drought, and severe drought-stressed plants. ¹³C distribution was monitored for up to 6 h post-application. Under optimal water conditions, ¹³C was mainly allocated to young (sink) leaves. During drought stress, transport trends changed, prioritizing C allocation primarily to the roots and nodules to a lesser extent. Metabolite profiling identified drought- and tissue-specific variations in the levels of the major C and N compounds.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70276"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Fe-Graphitic Carbon Nitride in Improving the Oil Profile, Flowering and Biochemical Attributes in the \"Shengeh\" Olive Under Drought Stress.","authors":"Rahmatollah Gholami, Narjes Fahadi Hoveizeh, Seyed Morteza Zahedi, Nadire Pelin Bahadirli, Mohsen Padervand, Petronia Carillo","doi":"10.1111/ppl.70311","DOIUrl":"https://doi.org/10.1111/ppl.70311","url":null,"abstract":"<p><p>To evaluate the effect of foliar-applied metal-nanostructures on alleviating the drought stress effects in olive trees, this study investigates the impact of Fe-carbon nitride nanostructures on flowering, oil profile, and some biochemical markers of olive trees grown under different irrigation regimes. The results indicated that drought decreased inflorescence number per branch by 45%, flower number per inflorescence by 9%, perfect and imperfect flower number per inflorescence by 16% and 7%, respectively, inflorescence length by 73%, fruit number per branch by 49%, and perfect flower number per branch by 54%. Whereas, the flowering parameters improved with Fe₂O₃/g-C₃N₄ treatment. Spraying treatments had a significant effect on fatty acid composition, whereas irrigation level and their interaction were not statistically significant. The Fe₂O₃/g-C₃N₄ treatment increased the concentrations of all investigated fatty acids. In contrast, irrigation regimes led to a decrease in C16:0, C18:0, C18:2, C18:3, and C20:0, while increasing C16:1 and C18:1. Nevertheless, the Fe₂O₃/g-C₃N₄ × 100%ET treatment numerically increased C18:0 and C18:2; whereas the Fe₂O₃/g-C₃N₄ × 75%ET treatment resulted in higher levels of C16:0, C16:1, C18:1, and C18:3. Drought stress caused a decrease of total chlorophylls by 62%, carotenoid by 96%, total flavonoid by 50%, and antioxidant capacity by 55%, while enhancing the phenol content by 115%. On the contrary, the use of Fe₂O₃/g-C₃N₄ increased total chlorophylls by 163%, phenol content by 148%, and antioxidant capacity by 34% as compared to the control. According to this research, the use of carbon nanostructure could represent a promising and cost-effective strategy to increase the olive tree tolerance to water deficit.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70311"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitric Oxide Dynamics in High-Altitude Medicinal Plants: Role in Stress Adaptation, Signaling, and Phytohormonal Interactions.","authors":"Shagun Sharma, Shivanti Negi, Pankaj Kumar, Mohammad Irfan","doi":"10.1111/ppl.70342","DOIUrl":"https://doi.org/10.1111/ppl.70342","url":null,"abstract":"<p><p>Nitric oxide (NO) is a pivotal signaling molecule that plays a crucial role in the high-altitude adaptation and stress tolerance of medicinal plants. Under hypoxic conditions prevalent in high-altitude environments, NO facilitates oxygen sensing, regulates mitochondrial function, and influences ethylene biosynthesis, thereby enhancing plant resilience. This review elucidates NO's modulation of oxidative stress responses through interactions with reactive oxygen species (ROS) to maintain redox homeostasis. Additionally, NO's interaction with key phytohormones, including abscisic acid, ethylene, and gibberellins, is examined, highlighting its role in hormone-mediated stress adaptation. Numerous medicinal plants adapted to high altitudes demonstrate NO's influence on secondary metabolite production, growth, and reproductive processes. The intricate crosstalk between NO and other signaling molecules, such as gasotransmitters and secondary messengers, reveals a complex network of integrative signaling pathways facilitating adaptive responses. Furthermore, advancements in NO detection and quantification techniques, along with existing challenges, are reviewed to provide insights into enhancing the understanding and application of NO dynamics in plant stress tolerance. In conclusion, this synthesis of current knowledge proposes potential biotechnological applications and future research directions to further explore NO-mediated adaptation mechanisms in medicinal plants thriving in high-altitude environments.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70342"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural and Functional Diversity of Glutamate Receptors-Like Channels in Plants.","authors":"Bisma Riaz, Yanli Zhang, Adeel Riaz, Wei Jiang, Hafiza Sadia, Yuan Qin, Guang Chen, Zhong-Hua Chen, Fenglin Deng, Fanrong Zeng","doi":"10.1111/ppl.70313","DOIUrl":"https://doi.org/10.1111/ppl.70313","url":null,"abstract":"<p><p>Glutamate receptor-like (GLR) family encodes cation-permeable ion channels that are crucial for defense signaling and have attracted significant research interest. The identification of multiple GLRs subfamilies across vascular lineages suggests their functional diversity in plants. Functional studies of clade 3 GLRs confirm their critical role in generating electrical signals and increasing cytosolic Ca²⁺ in response to mechanical wounding, insect and pathogen attacks, and other environmental cues for systemic defense responses. In this review, we present evidence that GLRs are conserved across all plant lineages and likely originated from Streptophyta. Comparative bioinformatic analysis of GLRs' functional domains reveals that ion channel gating and ligand binding of GLR are highly conserved and involved in ion transport and cell-to-cell communication in plants. The role of GLRs in electrical and Ca²⁺ signaling is also discussed in non-vascular tissues as well as in vascular plants. The hypothetical model suggests that GLR-induced systemic defense responses to external stimuli may have co-evolved with plant vascular systems. We also highlight the role of glutamate and other amino acid agonists in regulating membrane depolarization and cytosolic Ca²⁺ concentration. Finally, we review the roles of GLR in physiological processes, abiotic and biotic stresses, and strategies to enhance plant health and productivity.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70313"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Birch Salt Tolerance Using Gene Regulatory Network Highlighting Hormone Signaling, Reactive Oxygen Species Scavenging, and Ion Homeostasis.","authors":"Jingxin Wang, Xiaomeng Sun, Pengyu Wang, Siqi Wu, Yucheng Wang","doi":"10.1111/ppl.70331","DOIUrl":"https://doi.org/10.1111/ppl.70331","url":null,"abstract":"<p><p>Salt stress presents a formidable challenge for plant survival, yet the intricate regulatory networks dictating salt-tolerant gene expression remain elusive. This research delineates a gene regulatory network (GRN) in birch (Betula platyphylla) under salt stress, utilizing a partial correlation coefficient-based algorithm. The GRN comprises three hierarchical layers: the top layer with 5 transcription factors (TFs), the middle layer with 22 TFs, and the bottom layer encompassing 345 structural genes, totaling 1458 regulatory interactions. Validation through ChIP-PCR and qRT-PCR confirmed approximately 87.5% and 68.7% accuracy of predicted interactions in the top-middle and middle-bottom layers, respectively. The GRN underscores the pivotal roles of abscisic acid (ABA), jasmonic acid (JA), and cytokinin (CK) signaling pathways, emphasizing ROS scavenging and ion homeostasis as critical for salt tolerance. Among the top layer TFs, BpERF105 demonstrated superior salt tolerance, positioning it as a key regulatory element. This study posits that birch's salt tolerance is orchestrated through a regulatory homeostasis mediated by intricate TF-TF and TF-DNA interactions, providing profound insights into the molecular underpinnings of plant salt stress responses.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70331"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Iron Deficiency on the Arabidopsis thaliana Phloem Sap Proteome, a Key Role for bHLH121.","authors":"Berger Nathalie, Kalra Muskan, Gao Fei, Rofidal Valérie, Demolombe Vincent, Santoni Véronique, Dubos Christian","doi":"10.1111/ppl.70336","DOIUrl":"10.1111/ppl.70336","url":null,"abstract":"<p><p>Iron (Fe) is an essential micronutrient for plant growth and development whose homeostasis must be tightly regulated to avoid deficiency or excess that could be detrimental to the cells. In Arabidopsis thaliana, this mechanism is regulated by a series of transcription factors that act in an intricate regulatory network among which URI/bHLH121 (UPSTREAM REGULATOR OF IRT1) plays a predominant role. Tremendous efforts were deployed to decipher the molecular mechanisms that regulate iron homeostasis in plants. Nonetheless, the nature of the long-distance signal that conveys, via the phloem sap, information on the iron status of aerial tissues to the roots in order to coordinate iron uptake with the plant needs for iron is still to be determined. With the aim to identify potential actors involved in this process, we set up a proteomic analysis of the phloem sap of wild type Arabidopsis plants and bhlh121 loss-of-function mutants grown in iron-replete and iron-deficient conditions. We found that modifications in iron availability or the loss of URI activity have a profound impact on the phloem sap protein composition. We also found that some proteins whose translocation through the phloem sap is inhibited in response to iron deficiency are also affected in bhlh121 mutants. Interestingly, we discovered that some of the genes encoding such proteins are direct targets of URI, which suggests that the encoded proteins might act as potential signaling factors to regulate root iron uptake and/or root growth.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70336"},"PeriodicalIF":5.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12177908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}