The MpANT-Auxin Loop Modulates Marchantia polymorpha Development.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Dipp-Álvarez Melissa, Lorenzo-Manzanarez J Luis, Flores-Sandoval Eduardo, Espinal-Centeno Annie, Méndez-Álvarez Domingo, Fisher J Tom, León-Ruiz Jesús, Olvera-Martínez Fernando, Bowman L John, Arteaga-Vázquez A Mario, Alfredo Cruz-Ramírez
{"title":"The MpANT-Auxin Loop Modulates Marchantia polymorpha Development.","authors":"Dipp-Álvarez Melissa, Lorenzo-Manzanarez J Luis, Flores-Sandoval Eduardo, Espinal-Centeno Annie, Méndez-Álvarez Domingo, Fisher J Tom, León-Ruiz Jesús, Olvera-Martínez Fernando, Bowman L John, Arteaga-Vázquez A Mario, Alfredo Cruz-Ramírez","doi":"10.1111/ppl.70365","DOIUrl":null,"url":null,"abstract":"<p><p>AINTEGUMENTA-LIKE/PLETHORA/BABYBOOM (APB) genes are considered part of the ancestral developmental toolkit in land plants. In Arabidopsis thaliana, these transcription factors are induced by auxin and are primarily expressed in tissues with actively dividing cells, where they play essential roles in organ development. Marchantia polymorpha, a liverwort that diverged from A. thaliana early in embryophyte evolution, possesses a single APB ortholog, MpAINTEGUMENTA (MpANT), encoded in its genome. In this study, we aimed to characterize the function of MpANT. Analysis of a transcriptional fusion line indicates that MpANT is predominantly expressed in the meristematic region. We report that the MpANT promoter region contains several cis-acting Auxin Responsive Elements (AREs) and demonstrate that its expression, which occurs predominantly in meristematic regions, is significantly altered by the addition of exogenous auxin and inhibition of auxin transport. These findings indicate that MpANT acts downstream of Auxin Response Factors (ARFs) and auxin signaling. Analyses of loss- and gain-of-function MpANT alleles highlight the importance of this transcription factor in meristem maintenance and cell proliferation. Additionally, we found that MpANT acts upstream of the auxin transporter MpPIN1 by influencing auxin distribution. Taken together, our findings reveal a feedforward regulatory loop involving auxin, MpANT, and MpPIN1 that is important for Marchantia development.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 4","pages":"e70365"},"PeriodicalIF":5.4000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70365","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

AINTEGUMENTA-LIKE/PLETHORA/BABYBOOM (APB) genes are considered part of the ancestral developmental toolkit in land plants. In Arabidopsis thaliana, these transcription factors are induced by auxin and are primarily expressed in tissues with actively dividing cells, where they play essential roles in organ development. Marchantia polymorpha, a liverwort that diverged from A. thaliana early in embryophyte evolution, possesses a single APB ortholog, MpAINTEGUMENTA (MpANT), encoded in its genome. In this study, we aimed to characterize the function of MpANT. Analysis of a transcriptional fusion line indicates that MpANT is predominantly expressed in the meristematic region. We report that the MpANT promoter region contains several cis-acting Auxin Responsive Elements (AREs) and demonstrate that its expression, which occurs predominantly in meristematic regions, is significantly altered by the addition of exogenous auxin and inhibition of auxin transport. These findings indicate that MpANT acts downstream of Auxin Response Factors (ARFs) and auxin signaling. Analyses of loss- and gain-of-function MpANT alleles highlight the importance of this transcription factor in meristem maintenance and cell proliferation. Additionally, we found that MpANT acts upstream of the auxin transporter MpPIN1 by influencing auxin distribution. Taken together, our findings reveal a feedforward regulatory loop involving auxin, MpANT, and MpPIN1 that is important for Marchantia development.

mpant -生长素环调控多形地豆发育。
AINTEGUMENTA-LIKE/ /BABYBOOM (APB)基因被认为是陆地植物祖先发育工具包的一部分。在拟南芥中,这些转录因子由生长素诱导,主要在细胞分裂活跃的组织中表达,在器官发育中发挥重要作用。多态地茅(Marchantia polymorpha)是一种在早期胚胎进化中从拟南芥(a.thaliana)分化出来的地茅,其基因组中编码有一个单一的APB同源物MpAINTEGUMENTA (MpANT)。在这项研究中,我们旨在表征MpANT的功能。对转录融合系的分析表明,MpANT主要在分生组织区域表达。我们报道了MpANT启动子区域包含几个顺式作用的生长素响应元件(AREs),并证明其表达主要发生在分生组织区域,通过添加外源生长素和抑制生长素运输而显着改变。这些发现表明MpANT作用于生长素反应因子(ARFs)和生长素信号传导的下游。对MpANT等位基因功能缺失和功能获得的分析强调了这种转录因子在分生组织维持和细胞增殖中的重要性。此外,我们发现MpANT通过影响生长素的分布而作用于生长素转运体MpPIN1的上游。综上所述,我们的研究结果揭示了一个涉及生长素、MpANT和MpPIN1的前馈调节回路,这对Marchantia的发育很重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信