Physiologia plantarum最新文献_第6页

SEEDSTICK Affects Seed Development by Mediating Cytokinin Levels in Cotton. SEEDSTICK 通过调节棉花中的细胞分裂素水平影响种子发育。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70161

Xiaohong Zhang, Yifan Li, Yunjie Ge, Yuhao Mao, Genhai Hu, Qifeng Ma, Eryong Chen

{"title":"SEEDSTICK Affects Seed Development by Mediating Cytokinin Levels in Cotton.","authors":"Xiaohong Zhang, Yifan Li, Yunjie Ge, Yuhao Mao, Genhai Hu, Qifeng Ma, Eryong Chen","doi":"10.1111/ppl.70161","DOIUrl":"10.1111/ppl.70161","url":null,"abstract":"The SEEDSTICK transcription factor is important for flower and seed development, but the underlying molecular mechanisms remain unclear in cotton. In this study, we identified and cloned two STK homolog genes in upland cotton, an economically valuable cultivated crop. Phylogenetic and sequence analyses showed that the C-terminus of both GhSTKs had a conserved -DJJILHLG amino acid sequence and that GhSTK1 and GhSTK2 were very similar to GaAGL11 and GrAGL11, respectively. Quantitative real-time PCR analysis revealed that both GhSTKs were highly expressed in the ovules, and GUS activity was detected in the style and stigma. Subcellular localization experiments showed that GhSTK1 and GhSTK2 were localized to the nucleus. In Arabidopsis, the overexpression of GhSTK1 or GhSTK2 affected floral organ development and seed formation by increasing the transcript levels of the CKX genes and other genes related to floral development. Silencing both GhSTK1 and GhSTK2 increased the expression of GhFT and GhSHP and led to the earlier appearance of cotton buds. Yeast two-hybrid and bimolecular fluorescence complementation assays indicated that the two GhSTK proteins could interact with the GhSEP3 and GhSEP4 proteins. The present results suggest that GhSTK1 and GhSTK2, which have different sequences and expression patterns, might be functionally redundant and influence the regulation of cotton bud and seed development.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70161"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capacity to form common mycorrhizal networks reduces the positive impact of clonal integration between plants. 形成共同菌根网络的能力降低了植物间无性系整合的积极影响。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70149

Yuanhao Zhang, Mark A Anthony, Qianfeng Yuan, Yi Wang, Panpan Zhao, Enjian Chen, Shaolin Peng

{"title":"Capacity to form common mycorrhizal networks reduces the positive impact of clonal integration between plants.","authors":"Yuanhao Zhang, Mark A Anthony, Qianfeng Yuan, Yi Wang, Panpan Zhao, Enjian Chen, Shaolin Peng","doi":"10.1111/ppl.70149","DOIUrl":"10.1111/ppl.70149","url":null,"abstract":"Both clonal plant capabilities for physiological integration and common mycorrhizal networks (CMNs) formed by arbuscular mycorrhizal fungi (AMF) can influence the distribution of nutrients and growth among interconnected individuals. Using a microcosm model system, we aimed to disentangle how CMNs interact with clonal integration to influence plant growth and development. We grew Sphagneticola trilobata clones with isolated root systems in individual, adjacent containers while preventing, disrupting, or allowing clonal integration aboveground via spacers and belowground CMNs to form. We assessed multiple metrics of plant development (e.g., growth, specific leaf area, soluble sugar content), 15N transfer from donor (mother) to receiver (daughter) plants, and variation in AMF communities. We show that spacer formation between ramets and the capacity to form CMNs promoted and inhibited the growth of smaller daughter plants, respectively. In contrast to the independent effects of CMNs and spacers, CMNs, in combination with spacers, significantly weakened the promotion of daughter plants by clonal integration. AMF species richness was also negatively correlated with overall plant growth. Our results demonstrate that two common modes of plant interconnection interact in non-additive ways to affect clonal plant integration and growth. These findings, based on Sphagneticola trilobata, question the underlying assumptions of the positive effects of both AMF CMNs and species richness in comparison to direct plant interconnections.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70149"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective bioprocess engineering to enhance omega-6 polyunsaturated fatty acid production from Arthrospira platensis. 有效的生物工艺工程提高了platarthrospira的omega-6多不饱和脂肪酸的产量。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70186

Siddhant Dubey, Reeta Rani Singhania, Praveen Kumar Ramanujam, Chiu-Wen Chen, Cheng-Di Dong, Anil Kumar Patel

{"title":"Effective bioprocess engineering to enhance omega-6 polyunsaturated fatty acid production from Arthrospira platensis.","authors":"Siddhant Dubey, Reeta Rani Singhania, Praveen Kumar Ramanujam, Chiu-Wen Chen, Cheng-Di Dong, Anil Kumar Patel","doi":"10.1111/ppl.70186","DOIUrl":"10.1111/ppl.70186","url":null,"abstract":"Contrary to the robustness of microbial ω-3 polyunsaturated fatty acid (PUFA) production, the microbial synthesis of ω-6 PUFAs remains challenging. The rising demand for ω-6 PUFAs, especially for pregnancy and infant formulas, calls for scalable and sustainable production methods. Arthrospira platensis, a rarely explored microalgae, shows promise as a platform for producing gamma linoleic acid (GLA) and linolenic acid (LA), key components of ω-6 PUFAs. This study employs a two-phase cultivation approach to enhance ω-6 PUFA production in A. platensis. The initial growth phase was optimized to maximize biomass, followed by a stress-induced phase to boost lipid and ω-6 PUFA accumulation. Notably, ω-6 producing strains like A. platensis are protein-rich and not a high oleaginous species, achieving over 15% total lipid content particularly is significant. Under optimized conditions, a maximum biomass of 4.9 g/L with a productivity rate of (0.233 g/L/day) was obtained at 8 K Lux light irradiance, with 2X nitrogen concentration and 4 mg/L phytohormones. The subsequent stress phase, involving 20 K Lux light, 10 mg/L FeSO4, and 1% glycerol, resulted in a lipid content of 22.8%. This approach led to a 2.4-fold and 1.5-fold increase in microalgal biomass and lipid content respectively. Moreover, C18:2 and C18:3 PUFAs reaching approx. 17.1 ± 0.06% and 24.1 ± 0.07%, respectively. This research promotes microalgae cultivation to meet rising ω-6 PUFA demand, aligning with sustainable development goal 3: Good health and well-being.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70186"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutations of the brassinosteroid biosynthesis gene HvDWARF5 enable balance between semi-dwarfism and maintenance of grain size in barley. 油菜素内酯生物合成基因HvDWARF5的突变使大麦在半矮化和维持籽粒大小之间取得平衡。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70179

Karolina Zolkiewicz, Jana Oklestkova, Beata Chmielewska, Damian Gruszka

{"title":"Mutations of the brassinosteroid biosynthesis gene HvDWARF5 enable balance between semi-dwarfism and maintenance of grain size in barley.","authors":"Karolina Zolkiewicz, Jana Oklestkova, Beata Chmielewska, Damian Gruszka","doi":"10.1111/ppl.70179","DOIUrl":"10.1111/ppl.70179","url":null,"abstract":"Brassinosteroids (BRs) are phytohormones which regulate various developmental processes in plants. They are exceptional phytohormones, as they do not undergo long-distance transport between plant organs. However, knowledge about the function of the enzymes that catalyse BR biosynthesis (particularly its early stages) in cereal crops remains limited. Therefore, this study identifies and analyses the function of the HvDWARF5 (HvDWF5) gene, involved in the early stage of BR biosynthesis in barley (Hordeum vulgare), an important cereal crop, using the TILLING (Targeting Induced Local Lesions IN Genomes) approach. The detailed functional analysis allowed for the identification of various mutations in different gene fragments. The influence of these mutations on plant architecture, reproduction, and yield was characterised. Moreover, effects of the missense and intron retention mutations on sequence and splicing of the HvDWF5 transcript, sequence and predicted structure of the encoded HvDWF5 enzyme, and accumulation of endogenous BR were determined. Some of the barley mutants identified in this study showed semi-dwarfism, a trait of particular importance for cereal breeding and yield. However, unlike other BR mutants in cereals, this did not negatively affect grain size or weight. It indicated that mutations in this gene allow for a balance between plant height reduction and maintenance of grain size. Thus, the results of this study provide a novel insight into the role of the HvDWF5 gene in the BR biosynthesis-dependent regulation of architecture and reproduction of the important cereal crop - barley.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70179"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous tryptophan enhances cold resistance of soybean seedlings by promoting melatonin biosynthesis. 外源色氨酸通过促进褪黑素生物合成增强大豆幼苗抗寒性。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70189

Chunyuan Ren, Tong Cheng, Jingrui Jia, Liang Cao, Wenjie Zhang, Shaoze Zhang, Wanting Li, Yuxian Zhang, Gaobo Yu

{"title":"Exogenous tryptophan enhances cold resistance of soybean seedlings by promoting melatonin biosynthesis.","authors":"Chunyuan Ren, Tong Cheng, Jingrui Jia, Liang Cao, Wenjie Zhang, Shaoze Zhang, Wanting Li, Yuxian Zhang, Gaobo Yu","doi":"10.1111/ppl.70189","DOIUrl":"10.1111/ppl.70189","url":null,"abstract":"Given the global climate change, soybean production is highly susceptible to low temperature. Although tryptophan, the synthesis precursors of melatonin and auxin, exhibited a positive effect in regulating plant growth, it is still unclear whether tryptophan could improve the tolerance of soybean to low temperature stress through endogenous melatonin synthesis. Therefore, the effect of tryptophan on the resistance of two varieties of soybean seedlings to low temperature (4°C) was evaluated, and the main regulation pathway of tryptophan was verified with melatonin synthesis inhibitors. The results revealed that low temperature stress significantly inhibited the growth of soybean, while the application of exogenous tryptophan significantly enhanced the antioxidant activity of soybean seedlings to reduce the content of reactive oxygen species, including O2 - (11.3%) and H2O2 (17.8%), and effectively protected the photosynthetic capacity of leaves, involving net photosynthetic rate (22.94%), transpiration rate (15.31%), stomatal conductance (20.27%). And the application of tryptophan significantly increased the leaf area (16.63%), plant height (7.14%), root surface area (24.37%), root volume (22.92%) and root tip number (29.67%) of seedlings at low temperature. However, p-chlorophenylalanine inhibited the synthesis of melatonin and eliminated the effect of tryptophan. In conclusion, tryptophan mainly improved the cold tolerance of soybean seedlings by promoting endogenous melatonin synthesis, which provided a theoretical basis for tryptophan to enhance the cold tolerance of soybean in field production.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70189"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reassessing Drought Tolerance in Citrus Tetraploid Rootstocks: Myth or Reality? 重新评估柑橘四倍体砧木的耐旱性：神话还是现实？

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70199

Lucas da Silva Costa, Luciano Freschi, Maurício Antonio Coelho Filho, Monique Ayala Araújo da Silva, Fernanda Dos Santos Nascimento, Abelmon da Silva Gesteira

{"title":"Reassessing Drought Tolerance in Citrus Tetraploid Rootstocks: Myth or Reality?","authors":"Lucas da Silva Costa, Luciano Freschi, Maurício Antonio Coelho Filho, Monique Ayala Araújo da Silva, Fernanda Dos Santos Nascimento, Abelmon da Silva Gesteira","doi":"10.1111/ppl.70199","DOIUrl":"10.1111/ppl.70199","url":null,"abstract":"Polyploidy, particularly tetraploidy, has emerged as a promising tool in citrus rootstock breeding due to its potential to enhance drought tolerance. This review examines the role of tetraploid rootstocks in drought resilience, focusing on molecular and physiological adaptations observed in controlled environments and field conditions. Tetraploids display traits such as increased abscisic acid (ABA) production, antioxidant defenses, and osmotic adjustments. However, these advantages often fail to translate into superior drought tolerance in field conditions, where competition for resources and environmental complexities significantly influence plant responses. Recent evidence suggests that methodological limitations in earlier studies, particularly in pots, may have overstated the benefits of tetraploids. Field studies indicate that diploids, with more extensive root systems and greater water extraction capacity, often outperform tetraploids under water stress. To advance citrus breeding, it is essential to standardize experimental approaches, control soil matric potential, and prioritize long-term studies. Identifying key genes and metabolic pathways associated with drought tolerance, along with the application of advanced tools such as CRISPR/Cas9, will enable the development of resilient rootstocks, ensuring sustainable citrus production amidst increasing water scarcity and climate change.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70199"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening CYP450 genes from Gleditsia japonica Miq. and identifying CYP710A157 and CYP71D752 functions in the catalysis of echinocystic acid and betulin. 筛选 Gleditsia japonica Miq.的 CYP450 基因，确定 CYP710A157 和 CYP71D752 在催化棘囊酸和白桦脂中的功能。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70176

Ziyu Meng, Ming Dong, Changyixin Xiao, Ying Li, Yuqi Zhang, Jiale Cui, Siyao Wang, Peng Li, Dmitry Baleev, Yaguang Zhan, Jing Yin

{"title":"Screening CYP450 genes from Gleditsia japonica Miq. and identifying CYP710A157 and CYP71D752 functions in the catalysis of echinocystic acid and betulin.","authors":"Ziyu Meng, Ming Dong, Changyixin Xiao, Ying Li, Yuqi Zhang, Jiale Cui, Siyao Wang, Peng Li, Dmitry Baleev, Yaguang Zhan, Jing Yin","doi":"10.1111/ppl.70176","DOIUrl":"10.1111/ppl.70176","url":null,"abstract":"The triterpenes and saponin compounds of Gleditsia japonica Miq. play a key role in the suppression of various human tumor cell lines. Cytochrome P450 monooxygenases (CYP450s) are critical for the triterpene skeleton diversification and functional modification. This study systematically analyzed 104 full-length GjCYP450 genes in G. japonica from northeast China, classifying them into nine clans using bioinformatics. Co-expression modules and response patterns of GjCYP450s with triterpene pathway genes were constructed. Four genes - CYP710A157, CYP714E97, CYP716A377, and CYP71D752 - were selected for functional studies based on their high expression in different tissues of G. japonica and their homology with triterpenoid-related CYP450s in Arabidopsis thaliana. Co-expression of the CYP710A157 gene with the BpY gene (encoding β-amyrin synthase), and CYP71D752 with the BpW gene (encoding lupeol synthase) in tobacco significantly enhanced the catalytic efficiency of echinocystic acid (EA) and betulin (BT) compared to the control, by achieving 10.22-fold and 3.73-fold increases, respectively. Overexpression of CYP710A157 and CYP71D752 in Saccharomyces cerevisiae JWy602 yielded EA and BT at 3.25 mg l-1 and 13.84 mg l-1, respectively, whereas no product accumulation was detected in the control. Additionally, CYP710A157 and CYP714E97 enhanced yeast alkaline tolerance (500 mmol l-1 Na2CO3), while CYP716A377 and CYP71D752 improved their salt tolerance (10% NaCl). We reported the catalytic activity of CYP450 genes responsible for EA and BT synthesis within the CYP710A and CYP71D subfamilies in G. japonica for the first time here. These findings provide valuable genetic resources for plants' triterpene biosynthesis, including ginsenosides, and betulinic acid, and insights into regulating the triterpene metabolic network in G. japonica.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70176"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Auxin biodynamics and its integral role in enhancing plant resilience to environmental cues. 生长素的生物动力学及其在提高植物对环境的适应能力中的重要作用。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70165

Muhammad Ali, Linjuan Shi, Muhammad Aamir Khan, Ahmad Ali, Shuai Hu, Jinbo Shen

{"title":"Auxin biodynamics and its integral role in enhancing plant resilience to environmental cues.","authors":"Muhammad Ali, Linjuan Shi, Muhammad Aamir Khan, Ahmad Ali, Shuai Hu, Jinbo Shen","doi":"10.1111/ppl.70165","DOIUrl":"10.1111/ppl.70165","url":null,"abstract":"Auxins are essential plant hormones that regulate growth, development, and responses to environmental stressors. Plants frequently encounter challenges such as pests, diseases, high temperatures, drought, and salinity, which necessitate adaptive mechanisms for survival. Auxins modulate stress-responsive signaling pathways by regulating gene expression and interacting with other phytohormones, thereby influencing physiological processes that maintain homeostasis under stress conditions. This review elucidates the molecular mechanisms through which auxins mediate plant responses to biotic and abiotic stresses. The findings indicate that auxins are pivotal in activating defense mechanisms and regulating stress signaling pathways. Differential expression of auxin-related genes has been observed in various crops under stress conditions, underscoring their role in enhancing resistance against pathogens and improving drought tolerance. Additionally, auxins influence root architecture and growth responses, facilitating adaptations such as trichome development for defense against herbivory. Moreover, the interplay between auxin signaling and other phytohormones is crucial for effective stress responses. Overall, auxins play a multifaceted role in enabling plants to cope with environmental stresses by regulating growth and activating defense mechanisms. Understanding these complex signaling pathways involving auxins can inform future research aimed at engineering resilient plant varieties capable of thriving in changing climates. Further studies are needed to clarify the specific functions of auxin in various stress contexts and to develop practical applications for crop improvement.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70165"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A suitable strategy to find IAA metabolism mutants. 寻找IAA代谢突变体的合适策略。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70166

Rubén Casanova-Sáez, Aleš Pěnčík, Rafael Muñoz-Viana, Federica Brunoni, Rui Pinto, Ondřej Novák, Karin Ljung, Eduardo Mateo-Bonmatí

{"title":"A suitable strategy to find IAA metabolism mutants.","authors":"Rubén Casanova-Sáez, Aleš Pěnčík, Rafael Muñoz-Viana, Federica Brunoni, Rui Pinto, Ondřej Novák, Karin Ljung, Eduardo Mateo-Bonmatí","doi":"10.1111/ppl.70166","DOIUrl":"10.1111/ppl.70166","url":null,"abstract":"Indole-3-acetic acid (IAA), the most common form of auxin, is involved in a great range of plant physiological processes. IAA is synthesized from the amino acid tryptophan and can be transported and inactivated in a myriad of ways. Despite intense research efforts, there are still dark corners in our comprehension of IAA metabolism and its interplays with other pathways. Genetic screens are a powerful tool for unbiasedly looking for new players in a given biological process. However, pleiotropism of auxin-related phenotypes and indirect effects make it necessary to incorporate additional screening steps to specifically find mutants affected in IAA homeostasis. We previously developed and validated a high-throughput methodology to simultaneously quantify IAA, key precursors, and inactive forms from as little as 10 mg of fresh tissue. We have carried out a genetic screening to identify mutants involved in IAA metabolism. Auxin reporters DR5pro:VENUS and 35Spro:DII-VENUS were EMS-mutagenized and subjected to a parallel morphological and reporter-signal pre-screen. We then obtained the auxin metabolite profile of 325 M3 selected lines and used multivariate data analysis to identify potential IAA-metabolism mutants. To test the screening design, we identified the causal mutations in three of the candidate lines by mapping-by-sequencing: dii365.3, dii571.1 and dr693. These carry new alleles of CYP83A1, MIAO, and SUPERROOT2, respectively, all of which have been previously involved in auxin homeostasis. Our results support the suitability of this approach to find new genes involved in IAA metabolism.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70166"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of small secreted peptide signaling during defense response: insights from vascular and non-vascular plants. 防御反应中小分泌肽信号的比较分析：来自维管植物和非维管植物的见解。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70147

Irina Lyapina, Daria Ganaeva, Eugene A Rogozhin, Ekaterina V Ryabukhina, Dmitry Yu Ryazantsev, Vassili Lazarev, Sabina E Alieva, Anna Mamaeva, Igor Fesenko

{"title":"Comparative analysis of small secreted peptide signaling during defense response: insights from vascular and non-vascular plants.","authors":"Irina Lyapina, Daria Ganaeva, Eugene A Rogozhin, Ekaterina V Ryabukhina, Dmitry Yu Ryazantsev, Vassili Lazarev, Sabina E Alieva, Anna Mamaeva, Igor Fesenko","doi":"10.1111/ppl.70147","DOIUrl":"10.1111/ppl.70147","url":null,"abstract":"Small secreted peptides (SSPs) play an important role in modulating immune responses in all land plants. However, the evolution of stress peptide signaling in different plant phyla remains poorly understood. Here, we compared the expression of SSP genes in the pathogen-induced transcriptomes of vascular and non-vascular plants. We found 13, 19, 15, and 28 SSP families that were differentially expressed during infection in Physcomitrium patens, Zea mays, Brassica napus, and Solanum tuberosum, respectively. A comparative study of peptide motifs and predicted three-dimensional structures confirmed the similarity of SSPs across the examined plant species. In both vascular and non-vascular plants. However, only the RALF peptide family was differentially regulated under infection. We also found that EPFL peptides, which are involved in growth and development processes in angiosperms, were differentially regulated in P. patens in response to pathogen infection. The search for novel immune-specific peptides revealed a family of PSY-like peptides that are differentially regulated during infection in P. patens. The treatment with synthetic tyrosine-modified and non-modified PSY, and PSY-like peptides, as well as recombinant EPFL and MEG, validated their roles in the immune response and growth regulation. Thus, our study showed the complex nature of SSP signaling and shed light on the regulation of SSPs in different plant lineages during infection.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70147"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0