Physiologia plantarum最新文献

{"title":"Low Levels of Nitric Oxide and Reactive Oxygen Species in Guard Cells Are Required for Stomatal Opening by Fusicoccin or Butyrate.","authors":"Pulimamidi Bharath, Shashibhushan Gahir, Deepak Saini, Padmaja Gudipalli, Agepati S Raghavendra","doi":"10.1111/ppl.70569","DOIUrl":"https://doi.org/10.1111/ppl.70569","url":null,"abstract":"<p><p>Stomata play a crucial role in controlling the rate of photosynthesis and transpiration. Both stomatal opening and closure depend on intricate mechanisms involving several signaling components. The rise in nitric oxide (NO), reactive oxygen species (ROS), and cytosolic pH is necessary for inducing stomatal closure. However, the role of NO and ROS during stomatal opening has not been critically studied. Fusicoccin (FC) and butyric acid (BA) are known to induce guard cell cytosolic acidification and stomatal opening. We conducted a comprehensive study on NO and ROS patterns during stomatal opening induced by FC or BA. Both FC and BA suppressed NO and ROS levels of the guard cells as indicated by specific fluorescent dyes. The external addition of GSNO (natural NO-generator) or H₂O₂ (source of ROS) significantly suppressed FC- or BA-induced stomatal opening, confirming the requirement of low NO and ROS levels for stomatal opening. In addition, FC and BA lowered the guard cell pH as indicated by the fluorescent indicator, BCECF-AM. The ability of vanadate (PM-ATPase inhibitor) to restrict FC- or BA-induced opening suggested the importance of PM-ATPase-mediated cytosolic acidification, followed by suppression of NO and ROS levels in guard cells during stomatal opening. Further, RT-PCR analysis confirmed the upregulation of PM-ATPase by FC or BA. We propose that the guard cell acidification by FC or BA, due to PM-ATPase, caused the suppression of NO and ROS levels in guard cells and facilitated stomatal opening.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70569"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AtSnRK2.4 Functions as an ABA-Responsive Protein Kinase in Arabidopsis.","authors":"Mattia Adamo, Colette Tournaire-Roux, Valérie Rofidal, Philippe Nacry, Vincent Demolombe, Amandine Crabos, Christophe Maurel, Véronique Santoni","doi":"10.1111/ppl.70574","DOIUrl":"https://doi.org/10.1111/ppl.70574","url":null,"abstract":"<p><p>In Arabidopsis, members of subclasses I and III of sucrose non-fermenting 1-related subfamily protein kinases 2 (SnRK2) are considered to be mainly osmotic- and ABA-responsive, respectively. In this work, we report on the role of SnRK2.4, a member of subclass I, in shaping plant root architecture (e.g., lateral root growth and root primordia emergence) in response to exogenous ABA. We show that SnRK2.4 is active in standard conditions and upon ABA treatment, with a higher ABA sensitivity than SnRK2.2 and SnRK2.3 from class III. To identify the molecular substrates of SnRK2.4, we compared the transcriptome, proteome, and phosphoproteome of wild-type and snrk2.4 plants, in standard conditions and after a 1 μM ABA treatment. The phosphoproteomic analysis, which relies on 3858 unique phosphopeptides corresponding to 1820 phosphoproteins, revealed that 186 and 277 proteins were under-phosphorylated in snrk2.4 mutants, in control conditions and upon ABA treatment, respectively. A regulation by SnRK2.4 of membrane transporters and cell-to-cell communication was highlighted in both conditions. By contrast, in response to ABA, SnRK2.4 specifically induced a decreased abundance of RNA helicases, suggesting that SnRK2.4 can interfere with mRNA splicing. SnRK2.4 also modulated the phosphorylation of proteins putatively involved in attenuation of ABA signaling, in lipid signaling, and in cellulose biosynthesis, via a complex PK cascade involving mainly calcium-dependent PKs. This work shows that SnRK2.4 is an ABA-responsive SnRK2, with high hormone sensitivity and putative roles in fundamental aspects of cell physiology.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70574"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene Discovery and Development of Functional Markers for MYMIV Resistance in Urdbean (Vigna mungo L. Hepper).","authors":"Debjyoti Sen Gupta, Sankar Prasad Das, Sarbani Banik, Shivam Kumar, Jegadeesan Souframamien, Jitendra Kumar, Ashok Kumar Parihar, Ayam Gangarani Devi, Khela Ram Soren, Aravind Konda, Anup Chandra, Archana Singh, Pardip Kumar Katiyar, Rekha Das, Mallar K Nath, Girish Prasad Dixit","doi":"10.1111/ppl.70477","DOIUrl":"https://doi.org/10.1111/ppl.70477","url":null,"abstract":"<p><p>Mungbean Yellow Mosaic India Virus (MYMIV) is a major disease of urdbean (black gram) crop, which is one of India's most widely consumed pulses. In the present study, RNA-Seq-mediated differential gene expression analysis was conducted between a resistant variety (PU-31) and a susceptible variety (LBG-17) of urdbeans for the identification of resistant genes for the MYMIV disease. This resulted in the generation of a total of 827,157,878 clean reads through NovaSeq 6000 sequencing from the 12 samples of resistant and susceptible varieties evaluated under control (disease-free) and treatment conditions (disease condition). A trinity-based de novo assembly was developed with 553,889 coding regions of the urdbean genome. A set of 16 transcripts related to disease resistance that had high expression values in the present study was analyzed for differential gene expression using RT-PCR. Among these, two validated transcripts, TRINITY_DN4785_c0_g1_i9 (F-box/LRR resistance gene) and TRINITY_DN21430_c0_g3_i1 (Tobacco Mosaic Virus resistance gene), were used to develop DNA-based PCR markers. Using Sanger sequencing, five SNPs were found for an \"F-box/LRR\" resistance gene and a single SNP in the case of the \"TMV\" resistance gene. Using these SNPs, polymorphic PCR usable primers were designed and validated in a panel of urdbean genotypes. Utilizing SNP-445 of the \"TMV\" resistance gene, CAPS (Cleaved Amplified Polymorphic Sequence) and TSP (Temperature Switch PCR) markers were developed, and a TSP marker using SNP-361 was developed for the \"F-box/LRR\" resistance gene. Several urdbean varieties examined in the present study were found to harbor resistance alleles of the \"TMV\" and \"F-box/LRR\" genes, which likely contribute to the durability of resistance in these varieties. The molecular markers developed could be readily applied in marker-assisted selection for MYMIV resistance genes in urdbean.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70477"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZmMYB104 Enhances Heat-Stress Tolerance by Activating ZmCAT2 Expression in Maize.","authors":"Hao Zhang, Qiyue Wang, Teng Zhou, Xiaoqian Qiu, Chenhui Ma, Jihong Zhang, Javed Hussain Sahito, Yang Liu, Jiawen Zhao, Juan Li, Xiao Guo, Geming Guo, Keying Wan, Xuehai Zhang, Jihua Tang, Dong Ding","doi":"10.1111/ppl.70478","DOIUrl":"https://doi.org/10.1111/ppl.70478","url":null,"abstract":"<p><p>Temperature fluctuations critically affect plant growth, but the molecular mechanisms that underlie heat-stress tolerance in maize (Zea mays L.) remain to be fully characterized. Here, we examined the role of the MYB transcription factor ZmMYB104 in thermotolerance regulation and identified its downstream target genes. Through molecular cloning and expression analysis, we demonstrated that ZmMYB104 transcription is induced by heat in maize seedlings. Subcellular localization assays confirmed its presence in the nucleus, and transactivation assays demonstrated its ability to activate transcription. Overexpression lines exhibited greater heat-stress resistance than wild-type. Integration of RNA sequencing and DNA affinity purification sequencing (DAP-seq) revealed that the catalase gene ZmCAT2 was a direct target of ZmMYB104. Electrophoretic mobility shift assays confirmed that ZmMYB104 bound to the ZmCAT2 promoter, and dual-luciferase reporter assays quantified its ability to activate ZmCAT2 transcription. Overexpression ZmMYB104-mediated upregulation of ZmCAT2 significantly increased hydrogen peroxide (H₂O₂) scavenging capacity under heat stress, effectively reducing reactive oxygen species accumulation and oxidative damage. These findings demonstrate that ZmMYB104 confers thermotolerance through direct transcriptional activation of the catalase gene ZmCAT2, which encodes a key enzyme in ROS detoxification. Our data provide the first evidence for a ZmMYB104-ZmCAT2 regulatory module that functions in plant heat-stress responses, advancing our understanding of the transcriptional networks that govern thermotolerance in cereal crops. The ZmMYB104-ZmCAT2 axis represents a promising genetic target for the development of climate-resilient maize varieties through molecular breeding strategies.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70478"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DDM1-Mediated DNA Methylation Regulates Leaf Serration in Arabidopsis thaliana.","authors":"Shahid Ali, Wanpeng Wang, Lishan Wang, Yile Guo, Linan Xie, Bowei Chen, Qingzhu Zhang","doi":"10.1111/ppl.70495","DOIUrl":"https://doi.org/10.1111/ppl.70495","url":null,"abstract":"<p><p>DNA methylation is a crucial epigenetic modification that is stably inherited across both mitotic and meiotic cell divisions in plants. It is regulated by multiple epigenetic pathways, and alterations in methylation can lead to phenotypic variation independent of changes in the DNA sequence. In this study, changes in DNA methylation triggered by the chromatin remodeler DDM1 (DECREASE IN DNA METHYLATION 1) were found to influence leaf phenotypes in Arabidopsis thaliana. The ddm1 mutation dramatically reduced genome-wide DNA methylation, particularly in transposable elements (TEs). Hypomethylation of TEs activated downstream genes, including DPA4 (DEVELOPMENT-RELATED PCG TARGET IN THE APEX 4), which suppresses CUC2 (CUP-SHAPED COTYLEDON 2), a key regulator of leaf serration. Importantly, methylation changes in the DPA4 promoter were stably inherited in the F₂ generation. This study identified DPA4 as a gene affected by the ddm1 mutation, which significantly affects leaf serration in A. thaliana. These findings highlight the impact of induced epigenetic modifications on plant development, providing a basis for further exploration of heritable epigenetic traits.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70495"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Open-Source Digital Resources for 3D Visualization of Clustered Transcriptomic Data.","authors":"Hunter F Strickland, Andrew Shen, Anna-Lisa Paul, Robert Ferl","doi":"10.1111/ppl.70500","DOIUrl":"10.1111/ppl.70500","url":null,"abstract":"<p><p>As datasets grow in size with the increased accessibility of high-throughput transcriptome sequencing, methods of dimensionality reduction have become invaluable for data analysis. The methods of dimensionality reduction, including t-distributed stochastic neighbor embedding or Uniform Manifold Approximation and Projection, are utilized to create figures and projections of the high-dimensional data into a set of lower dimensions, 2D or 3D, which are more well-suited for human comprehension. These methods of dimensionality reduction have continually grown in popularity and widespread use. Despite this popularity, creating engaging and visually attractive features remains an issue for many users without significant coding experience. To remediate this issue, an HTML-based digital resource was created that utilizes publicly available scripts from JsDelivr and GitHub, and Blender, an open-source modeling software. We have generated two open-source digital data visualization resources that can be applied to the transcriptomic data processed using the aforementioned methods of dimensionality reduction. The first, HTMLview, utilizes a provided HTML file template to create an interactive and engaging 3D model in digital space. The second method, Blenderview, utilizes the open-source modeling software, Blender, to create and animate high-quality models and videos of processed datapoints. The two methods were tested with transcriptomic data processed via dimensionality reduction algorithms. The methods provided create two distinct paths for researchers to better visualize, examine, and share their data, while also utilizing open-source technologies that are readily available to most potential users.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70500"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12441758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Structural Bridge Between Kingdoms: How Collagen-Derived Peptides Influence Plant Stress and Growth Pathways.","authors":"Stefano Ambrosini, Alejandro Giorgetti, Marika Peli, Tiziana Pandolfini, Anita Zamboni, Zeno Varanini","doi":"10.1111/ppl.70512","DOIUrl":"10.1111/ppl.70512","url":null,"abstract":"<p><p>Collagen-derived protein hydrolysates (CDPH) are widely used as plant biostimulants primarily due to their content of bioactive oligopeptides. When applied to hydroponically grown Solanum lycopersicum plants, CDPH significantly promoted root development, particularly by increasing the number and length of lateral roots. To gain insight into the underlying molecular mechanisms, we hypothesized that plants may possess proteins capable of interacting with collagen-like peptides. To test this, we conducted a comprehensive homology search of the Arabidopsis thaliana proteome using a Hidden Markov model-based approach built from three human collagen-binding proteins (CBPs) and 14 known collagen-binding domains (CBDs). After filtering, 10 Arabidopsis proteins emerged as putative candidates with the potential to bind collagen. Notably, the highest homology was observed for a matrix metalloproteinase, At5-MMP, showing 44% identity with its human counterpart HsMMP1, and for AtSERPIN1, which displayed the strongest e-value match to HsSERPINH1 (22% identity). Both plant proteins are functionally associated with responses to abiotic and biotic stresses, a feature that mirrors the known physiological effects of CDPH-based biostimulants. These findings support the hypothesis that plants possess proteins capable of recognizing collagen-like structures, offering a plausible molecular basis for the activity of CDPH-based biostimulants and paving the way for future biochemical validation.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70512"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12434152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tephritis Angustipennis-Induced Flavonoid Dynamics Drive Cross-Species Pest Resistance in Aster spp.","authors":"Li-Jun Zhang, Xin-You Wang, Ying Liu, Yan-Long Wang, Yu-Shou Ma","doi":"10.1111/ppl.70532","DOIUrl":"https://doi.org/10.1111/ppl.70532","url":null,"abstract":"<p><p>Flowering Aster species (Aster spp.) native to the high-altitude (3753 m on average) Three Rivers Source Region are frequently damaged by Tephritis angustipennis, imposing significant stress that negatively impacts their survival and productivity. This study reveals that pest stress enhances antioxidant enzyme activity (CAT, POD, SOD, and PPO) and increases secondary metabolites, particularly flavonoids, in Aster flowers. Integrated transcriptomic and metabolomic analyses identified differential responses among three Aster varieties exhibiting distinct resistance levels. We found that increasing pest stress, particularly under serious-hazard (HH) conditions, upregulates resistance-related genes and promotes the accumulation of phenolic and terpenoid compounds. Pest feeding activates the flavonoid biosynthesis pathway, with key enzyme genes (PAL, CAD, HCT, FLS, and CYP) being upregulated, reflecting a rapid physiological response that enhances resistance. Notably, flavonoid synthesis is dynamically regulated in response to stress. Under HH conditions, quercetin and kaempferol levels decrease, while phlorizin, kaempferide, sakuranetin, and isosakuranetin increase, indicating a complex defense strategy. Overall, pest-induced flavonoid accumulation helps delay the process of flower aging and deterioration, thereby enhancing seed yield. These findings provide valuable insights into the molecular defense mechanisms of Aster spp., offering potential targets for breeding pest-resistant varieties and developing effective pest management strategies.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70532"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating Transcriptomics and Metabolomics in Poplar to Uncover Crucial Genes and Metabolites in Response to Anoplophora glabripennis Infestation.","authors":"Tiantian Fu, Zeqi Wang, Chun Wang, Yuzhang Yang, Ruen Yu, Xiaoqian Yang, Jiahao Liu, Hongkun Ji, Yuanlong Wang, Tianliang Wang, Jieru Li, Yanwei Wang","doi":"10.1111/ppl.70523","DOIUrl":"https://doi.org/10.1111/ppl.70523","url":null,"abstract":"<p><p>Anoplophora glabripennis (ALB) is one of the most devastating stem-boring beetles. However, knowledge pertaining to the molecular regulation and key responses of poplars under ALB infestation remains limited. In this investigation, the transcriptomes and metabolomes of two different poplars with different resistances under ALB infestation were jointly analyzed, combined with phytohormone analysis. Two thousand one hundred ninety-five genes, including defense genes such as MYB44, NAC1, and DELLA, were significantly altered in both poplar varieties after ALB infestation. Notably, the elevated levels of JA accumulation, the differential expression of poplar-specific phenolic glycosides, and the significant accumulation of catechins in Populus tomentosa might be the key factors leading to the differential resistance. In addition, the key metabolic enzymes involved in the synthesis of catechins (DFR, LAR, 4CL, CHS, and F3H) were significantly upregulated in P. tomentosa. Intriguingly, the upregulation of numerous resistance genes and the downregulation of a few photosynthesis-related genes in resistant poplars reflect a trade-off strategy between defense and growth. Several transcription factors, including WRKY, were identified in the significant induction of SA and JA signaling pathways in poplars in response to ALB infestation. Taken together, this investigation marked an important step toward elucidating the insect resistance mechanisms in poplar and should provide crucial insights into the development of new insect-resistant poplar varieties.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70523"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Proteomic and Physiological Profiling of Phosphate Stress Response in Potato (Solanum tuberosum L.).","authors":"Lulu Xia, Lixiang Cheng, Qingquan Zhang, Feng Zhang","doi":"10.1111/ppl.70551","DOIUrl":"https://doi.org/10.1111/ppl.70551","url":null,"abstract":"<p><p>Inorganic phosphate (Pi) is an essential element for plant growth and development. To investigate the response of potatoes to Pi stress, five treatments of control (1.25 mM KH₂PO₄), low Pi treatments (0, 0.25, and 0.5 mM KH₂PO₄), and high Pi treatment (2.5 mM KH₂PO₄) were set up. The physiological results showed that both low and high Pi treatments inhibited the growth and development of potato plants. Low Pi treatments inhibited the yield and starch granule size of potato tubers, and there was no significant difference under the high Pi treatment. Two-dimensional gel electrophoresis (2-DE) and MALDI-TOF/TOF-MS mass spectrometry were used to identify 49 differentially expressed protein spots (p < 0.05, differential expression ≥ 2-fold) in potato leaves under different Pi treatments. Some primary carbon metabolism-related enzymes were up-regulated, and sufficient metabolic intermediates and energy were provided by low Pi treatments to enhance the resistance to Pi stress. Moreover, low Pi treatments induced more defense mechanisms than \"high Pi treatments\", resulting in enhanced resistance. Under Pi stress, although most photoreaction-related proteins were down-regulated, potato specifically induced the up-regulation of CO₂ fixation and assimilation-related enzymes to maintain growth and metabolism. Pi stress disrupted redox homeostasis, but potatoes achieved dynamic regulation of the antioxidant system by inducing synergistic up-regulation of some antioxidant enzymes. Finally, low Pi stress also activated the calcium signaling pathway, which may synergistically act with other signal transduction proteins to regulate Pi absorption, transport, and utilization in potatoes. These results provide important information on the response of potatoes to Pi stress.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70551"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}