Physiologia plantarum最新文献_第7页

Static and dynamic acclimation mechanisms to extreme light intensities in Hedera helix (Ivy) plants. 常青藤植物对强光的静态和动态驯化机制。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70217

Hagit Zer, Ayelet Zion Ben-Ami, Nir Keren

{"title":"Static and dynamic acclimation mechanisms to extreme light intensities in Hedera helix (Ivy) plants.","authors":"Hagit Zer, Ayelet Zion Ben-Ami, Nir Keren","doi":"10.1111/ppl.70217","DOIUrl":"https://doi.org/10.1111/ppl.70217","url":null,"abstract":"Under natural conditions, plants face the need to acclimate to widely varying light intensities to optimize photosynthetic efficiency and minimize photodamage. Studying the mechanisms underlying these acclimation processes is essential for understanding plant productivity and resilience under fluctuating environmental conditions. This study aimed to investigate static and dynamic acclimation mechanisms in Hedera helix (Ivy) plants under two extreme light conditions spanning the range of their adaptive abilities, deep shade (LL, ~5 μmol photons m-2 s-1) to full sunlight (HL, ~2000 μmol photons m-2 s-1), focusing on their structural and functional acclimation. LL and HL plants were examined for their leaf structure, chlorophyll and carotenoid contents, and photosynthetic protein levels. Dynamic responses were evaluated through chlorophyll fluorescence spectroscopy, measuring the effective photosynthetic unit size (σ) and the capacity for non-photochemical quenching (NPQ). HL plants exhibited a ~ 78% lower chlorophyll contents as compared to LL and increased chlorophyll a/b ratios. The carotenoid content of HL plants was ~94% lower, while the PsbS content increased fivefold. These results may indicate a smaller HL effective antenna size. However, σ fast fluorescence kinetics analysis indicated the opposite. NPQ analysis demonstrated that both compositions of the photosynthetic systems supported the ability to quench access energy. HL plants had a large dynamic range for NPQ and faster on/off kinetics. Our finding suggests massive changes in the organization of the photosynthetic apparatus. These modifications preserve a large dynamic range for reacting to light intensity under both conditions.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70217"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11998634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly variable mitochondrial chromosome content in a holoparasitic plant due to recurrent gains of foreign circular DNA. 由于外源环状DNA的反复获得，全寄生植物中高度可变的线粒体染色体含量。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70231

Leonardo Martin Gatica-Soria, M Emilia Roulet, Walter D Tulle, Hector A Sato, M Eugenia Barrandeguy, M Virginia Sanchez-Puerta

{"title":"Highly variable mitochondrial chromosome content in a holoparasitic plant due to recurrent gains of foreign circular DNA.","authors":"Leonardo Martin Gatica-Soria, M Emilia Roulet, Walter D Tulle, Hector A Sato, M Eugenia Barrandeguy, M Virginia Sanchez-Puerta","doi":"10.1111/ppl.70231","DOIUrl":"https://doi.org/10.1111/ppl.70231","url":null,"abstract":"Multichromosomal mitochondrial genomes (mtDNAs) in eukaryotes exhibit remarkable structural diversity, yet intraspecific variability and the origin of the individual chromosomes remain poorly understood. We focus on a holoparasitic angiosperm with an mtDNA consisting of 65 chromosomes largely composed of foreign DNA acquired by horizontal gene transfer (HGT) from its mimosoid hosts. The frequency, timing and population dynamics of these HGT events have not been examined. Here, we sampled different individuals of the holoparasite Lophophytum mirabile, along with their host plants, to assess mtDNA intraspecific variability and capture recent events that may bring insights into the HGT process. We also gathered mitochondrial data from 43 mimosoids to identify older and recent HGT events and assess precisely the proportion of foreign DNA. Through comparative genomic and evolutionary analyses, we uncovered great intraspecific variability in chromosome content and defined the mitochondrial pangenome of L. mirabile with 105 distinct chromosomes. The estimated foreign content reaches 93.5% of the mtDNA, including 73 fully foreign chromosomes that support the circle-mediated HGT model as a key mechanism for their acquisition. We inferred recurrent DNA transfers from the host plants, leading to new mitochondrial chromosomes that replicate autonomously. Our results emphasize the importance of adopting a pangenomic approach to fully capture the genetic diversity and evolution of multichromosomal mitochondrial genomes. This study shows that HGT can strongly influence the mtDNA content and generate enormous intraspecific variability even in geographically close individuals.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70231"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential impact of commercial rootstocks on the physiological response of a common walnut scion to drought stress. 商业砧木对普通核桃接穗干旱生理反应的差异影响。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70188

Mina Momayyezi, Thorsten Knipfer, Maria Isabel Hernandez-Perez, Daniel A Kluepfel, Collins Wakholi, Devin A Rippner, Caetano P Albuquerque, Nicolas E Bambach, Jack DeGrom, Andrew J McElrone

{"title":"Differential impact of commercial rootstocks on the physiological response of a common walnut scion to drought stress.","authors":"Mina Momayyezi, Thorsten Knipfer, Maria Isabel Hernandez-Perez, Daniel A Kluepfel, Collins Wakholi, Devin A Rippner, Caetano P Albuquerque, Nicolas E Bambach, Jack DeGrom, Andrew J McElrone","doi":"10.1111/ppl.70188","DOIUrl":"https://doi.org/10.1111/ppl.70188","url":null,"abstract":"Walnut rootstocks are commonly used in California orchards to provide resistance to soil-borne pests and diseases. However, little information exists about the impact of commercial rootstock on the common scion's physiological response under drought. This is becoming increasingly important since walnuts are commonly cultivated in semi-arid regions where frequent and severe droughts require efficient water use. We previously reported that own-rooted walnut rootstocks (RX1, VX211 and Vlach) differ in their physiological performance under drought. Here, we evaluated whether similar water relations and performance are conferred to a common English walnut scion (Juglans regia cv. Cisco). To do so, we used a mini-lysimeter platform to continuously track soil moisture and transpirational water loss from trees. Along with the canopy's estimated leaf area, changes in canopy shape and texture were evaluated using deep learning as an independent method to analyze canopy response to water stress. In support of our recent findings, the scion grafted onto rootstock RX1 exhibited subtle improvements in physiological performance associated with higher transpiration and canopy conductance under well-watered condition compared to Vlach and VX211 rootstocks. Canopy conductance, texture, and shape were not significantly affected by rootstock under water stress. However, Cisco grafted onto RX1 exhibited higher leaf turgor and water use efficiency, and lower osmotic potentials under water stress. Our results suggest some subtle differences in water relations between the rootstock genotypes, and propose an efficient deep-learning method to screen canopies for water stress-induced response through image processing.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70188"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergism of Bacillus subtilis and Azospirillum brasilense for enhanced N-use efficiency and maize growth: Evidence from ¹⁵N isotopic and physiological responses. 枯草芽孢杆菌和巴西氮螺旋菌对提高氮素利用效率和玉米生长的协同作用：来自15N同位素和生理反应的证据

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70205

Fernando Shintate Galindo, Cassio Carlette Thiengo, Paulo Humberto Pagliari, João Victor Silva Bernardes, Gustavo Duprat Dos Santos, Pedro Augusto Fedato Longato, Lucila de Sousa Vilela, Marcelo Carvalho Minhoto Teixeira Filho, José Lavres

{"title":"Synergism of Bacillus subtilis and Azospirillum brasilense for enhanced N-use efficiency and maize growth: Evidence from 15N isotopic and physiological responses.","authors":"Fernando Shintate Galindo, Cassio Carlette Thiengo, Paulo Humberto Pagliari, João Victor Silva Bernardes, Gustavo Duprat Dos Santos, Pedro Augusto Fedato Longato, Lucila de Sousa Vilela, Marcelo Carvalho Minhoto Teixeira Filho, José Lavres","doi":"10.1111/ppl.70205","DOIUrl":"https://doi.org/10.1111/ppl.70205","url":null,"abstract":"We explored the impact of inoculating maize (Zea mays L.) seeds with plant growth-promoting rhizobacteria (PGPR - Bacillus subtilis and Azospirillum brasilense), either individually or in combination, under different nitrogen input levels - control, low, average and high N levels (0, 30, 60, 90 and 120 mg L-1 equivalent to between 0 and 240 kg N ha-1) in a greenhouse setting. Leaf- and plant-level biometrics, nutritional, biochemical, and physiological evaluations were supplemented by isotopic methods (15N isotope dilution and natural abundance - δ15N‰) along with root scanning to investigate N acquisition and distribution. Dual inoculation not only enhanced the recovery of applied N but also bolstered nitrogenase activity, leading to increased biological N fixation (BNF) even at an average level of N (120 kg N ha-1). In instances where dual inoculated plants were grown at low (60 kg N ha-1) and average N levels, a cascade effect was observed, such as encompassing root growth stimulation, enhanced fertilizer and soil exploitation, and increased biomass production. Increasing N application to 240 kg N ha-1 reduced plant biomass by 19-47% compared to 120 kg N ha-1, depending on inoculation strategy. This led to downregulation of nitrogenase activity, diminished PGPR efficiency, and a significant decline in BNF. This study shows how a change in the rhizosphere microbial population can influence a myriad of responses inside the plants. Our research provides valuable insights for studies using 15N isotopic and dual inoculation techniques, offering strong potential for practical implementation.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70205"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the molecular mechanisms underlying flat stem formation in Atractylodes lancea in response to phytoplasmas. 白术在植物浆体作用下扁平茎形成的分子机制。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70192

Ling Gong, Lei Chen, Xiao Huang, Juan Deng, Xiaoyi Wu, Yating Hu, Meiling Jiang, Kun Yu, Changli Liu

{"title":"Unraveling the molecular mechanisms underlying flat stem formation in Atractylodes lancea in response to phytoplasmas.","authors":"Ling Gong, Lei Chen, Xiao Huang, Juan Deng, Xiaoyi Wu, Yating Hu, Meiling Jiang, Kun Yu, Changli Liu","doi":"10.1111/ppl.70192","DOIUrl":"https://doi.org/10.1111/ppl.70192","url":null,"abstract":"For centuries, the herb Atractylodes lancea (Thunb.) has been revered for its pharmacological properties, particularly its efficacy in combating spleen and stomach ailments. However, prolonged artificial cultivation of A. lancea has led to an increased presence of serious plant diseases, including flat branch disease caused by phytoplasmas. To elucidate the mechanisms of flat stem (FS) formation, physiological and biochemical indicators, as well as related metabolic pathways were assessed in A. lancea stems following phytoplasma infection. The results demonstrated significant changes in the levels of stress compounds H2O2 and MDA, as well as the activities of antioxidant enzymes APX, POD, PPO, and CAT. Additionally, the FS structures of A. lancea exhibited elevated levels of JA and zeatin, accompanied by a decrease in auxin content. High-throughput sequencing identified differentially expressed genes (DEGs) enriched in various pathways, including hormone biosynthesis, signal transduction, and Ca2+ signalling. These findings enhance our understanding of phytoplasma-induced FS development in A. lancea and provide a basis for future molecular studies.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70192"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MtMAP3Kδ6 Modulates the Growth and Development through Sugar Metabolism Regulation in Medicago truncatula. MtMAP3Kδ6通过糖代谢调控短叶苜蓿生长发育

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70208

Nan Ma, Haotian Li, Ting Liu, Hanwen Zhang, Jiangnan Yi, Cai Gao, Jiaxin Ma, Peizhi Yang, Tianming Hu, Yuman Cao

{"title":"MtMAP3Kδ6 Modulates the Growth and Development through Sugar Metabolism Regulation in Medicago truncatula.","authors":"Nan Ma, Haotian Li, Ting Liu, Hanwen Zhang, Jiangnan Yi, Cai Gao, Jiaxin Ma, Peizhi Yang, Tianming Hu, Yuman Cao","doi":"10.1111/ppl.70208","DOIUrl":"10.1111/ppl.70208","url":null,"abstract":"Plant growth and development are intricately regulated by molecular mechanisms, with the mitogen-activated protein kinase (MAPK) signaling cascade and its associated modules being pivotal. In this study, we identified and characterized a member of the MAPKKK family, MtMAP3Kδ6, from Medicago truncatula. This gene, classified within the B3 subgroup of the MAPKKK family, was expressed across various tissues during plant growth. The knockout mutant of MtMAP3Kδ6 displayed dwarfism, characterized by reduced branching and smaller leaf size, whereas overexpression of MtMAP3Kδ6 in Medicago truncatula led to the converse phenotypes. Transcriptome analysis and subsequent validation in leaves from different strains showed that the knockout mutants of MtMAP3Kδ6 had decreased levels of starch and sucrose, along with diminished cell wall invertase (INV; EC 3.2.1.26) activity, whereas overexpression resulted in the opposite effects. Collectively, our findings suggest that MtMAP3Kδ6 plays a role in Medicago truncatula growth and development by positively modulating sugar metabolism. This research lays a theoretical groundwork for future studies on the role of MAPKKK in sugar metabolism and its implications for plant growth and development.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70208"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maize mutants in miR394-regulated genes show improved drought tolerance. mir394调控基因的玉米突变体表现出更高的耐旱性。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70155

Franco Miskevish, Anabella Lodeyro, María Agustina Ponso, Carlos Bouzo, Robert Meeley, Marja C Timmermans, Marcela Dotto

{"title":"Maize mutants in miR394-regulated genes show improved drought tolerance.","authors":"Franco Miskevish, Anabella Lodeyro, María Agustina Ponso, Carlos Bouzo, Robert Meeley, Marja C Timmermans, Marcela Dotto","doi":"10.1111/ppl.70155","DOIUrl":"10.1111/ppl.70155","url":null,"abstract":"Water limitation represents one of the major threats to agricultural production, which often leads to drought stress and results in compromised growth, development and yield of crop species. Drought tolerance has been intensively studied in search of potential targets for molecular approaches to crop improvement. However, drought adaptive traits are complex, and our understanding of the physiological and genetic basis of drought tolerance is still incomplete. The miR394-LCR pathway is a conserved regulatory module shown to participate in several aspects of plant growth and development, including stress response. Here, we characterized the miR394 pathway in maize, which harbours two genetic loci producing an evolutionarily conserved mature zma-miR394 targeting two transcripts coding for F-Box proteins, named hereby ZmLCR1 and ZmLCR2. Arabidopsis plants overexpressing the zma-MIR394B gene showed high tolerance to drought conditions compared to control plants. Moreover, analysis of the growth and development of single and double maize mutant plants in ZmLCR genes indicate that these mutations do not affect plant fitness when they grow in normal watering conditions, but mutants showed better survival than wild-type plants under water deprivation conditions. This increased drought tolerance is based on more efficient intrinsic water use, changes in root architecture and increased epicuticular wax content under water-limiting conditions. Our results indicate that the miR394-regulated ZmLCR genes are involved in drought stress tolerance and are remarkable candidates for maize crop improvement.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70155"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex and salt stress response - physiological and biochemical aspects of hydroponic culture of dioecious Rumex thyrsiflorus Fingerh. 性别和盐胁迫反应——雌雄异株水培鸢尾的生理生化研究。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70204

Kacper Gozdur, Petronia Carillo, Agnieszka Szopa, Ireneusz Ślesak, Rosalinda Nicastro, Renata Biba, Julia Tyrajska, Halina Ślesak

{"title":"Sex and salt stress response - physiological and biochemical aspects of hydroponic culture of dioecious Rumex thyrsiflorus Fingerh.","authors":"Kacper Gozdur, Petronia Carillo, Agnieszka Szopa, Ireneusz Ślesak, Rosalinda Nicastro, Renata Biba, Julia Tyrajska, Halina Ślesak","doi":"10.1111/ppl.70204","DOIUrl":"https://doi.org/10.1111/ppl.70204","url":null,"abstract":"This study investigates the sex-specific physiological and biochemical responses to salt stress in male and female Rumex thyrsiflorus plants under hydroponic culture conditions. In vitro regenerated plants were exposed to different sodium chloride (NaCl) concentrations (0, 43, and 86 mM), and the resulting changes in morphology, photosynthetic performance, and biochemical profiles were analyzed. Salt stress resulted in significant morphological adaptations, including reduced leaf area and closed stomata, particularly in the male plants, indicating adaptive strategies to minimize water loss and ion toxicity. Photosynthetic efficiency, especially the photochemical performance of photosystem II, decreased under elevated NaCl levels, with a marked reduction observed at 86 mM. Biochemical analyses revealed remarkable responses, including increased enzymatic antioxidant activities and the accumulation of free proline, a known compatible osmolyte, as well as branched-chain amino acids, soluble proteins, and carbohydrates. These shifts in metabolite profiles varied by sex, with male plants showing a greater increase in compounds such as proline, γ-aminobutyric acid, methionine, and the osmoprotectant sucrose, highlighting sex-specific patterns of metabolic adaptation. Females showed higher chlorophyll retention and greater resistance to oxidative damage, suggesting a range of different adaptive strategies. The study highlights the importance of identifying sex-specific stress responses in R. thyrsiflorus, which has implications for breeding programmes aiming to improve crop resilience. These results expand our understanding of plant stress biology and provide valuable insights for further research into how dioecious plants respond to environmental challenges.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70204"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144043152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the production of chlorophyll f in the cyanobacterium Synechocystis sp. PCC 6803. 促藻蓝细菌pcc6803叶绿素f的生成

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70169

Man Qi, Henry N Taunt, Martina Bečková, Zhi Xia, Joko P Trinugroho, Josef Komenda, Peter J Nixon

{"title":"Enhancing the production of chlorophyll f in the cyanobacterium Synechocystis sp. PCC 6803.","authors":"Man Qi, Henry N Taunt, Martina Bečková, Zhi Xia, Joko P Trinugroho, Josef Komenda, Peter J Nixon","doi":"10.1111/ppl.70169","DOIUrl":"10.1111/ppl.70169","url":null,"abstract":"One potential approach to improve the productivity of cyanobacteria and microalgae is to enhance photosynthetic efficiency by introducing far-red absorbing pigment molecules (such as chlorophylls f and d) into the photosynthetic apparatus to expand the range of photosynthetically active radiation. We have shown previously that expressing the ChlF subunit of Chroococcidiopsis thermalis PCC 7203 in the model cyanobacterium Synechocystis sp. PCC 6803 (Syn6803) is sufficient to drive the production of chlorophyll f (Chl f), but only to low levels (0.24% Chl f/Chl a). By using the strong Pcpc560 promoter and an N-terminal truncated derivative of ChlF, we have been able to increase the yield of Chl f in white light by over 30-fold to about 8.2% Chl f/Chl a, close to the level displayed by far-red photoacclimated C. thermalis 7203. Additionally, we demonstrate that ChlF from Fisherella thermalis PCC 7521, like ChlF from C. thermalis 7203, assembles into a variant of the monomeric photosystem II (PSII) core complex termed the super-rogue PSII complex when expressed in Syn6803. This contrasts with the originally reported formation of a ChlF homodimeric complex in Synechococcus sp. PCC 7002. Overall, our work is an important starting point for mechanistic and structural studies of super-rogue PSII and for incorporating Chl f into the photosynthetic apparatus of Syn6803.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70169"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The TPX family of co-repressors: a hub amidst the hubbub. TPX家族的共同阻遏物：喧嚣中的中心。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-03-01 DOI: 10.1111/ppl.70185

Benjamin Downing, Alexander R Leydon, Jennifer L Nemhauser

{"title":"The TPX family of co-repressors: a hub amidst the hubbub.","authors":"Benjamin Downing, Alexander R Leydon, Jennifer L Nemhauser","doi":"10.1111/ppl.70185","DOIUrl":"10.1111/ppl.70185","url":null,"abstract":"The phytohormone auxin affects a wide range of plant responses through global shifts in gene expression. The TOPLESS/TOPLESS RELATED (TPL/TPR) co-repressors (here collectively called the TPX family for simplicity) play a central role in this transcriptional regulation, acting through a variety of mechanisms, including modifying chromatin accessibility and assembling the machinery needed for transcription initiation. Structure-function analysis has mapped multiple repression domains within the founding TPL protein, and uncovered several forms of post-translational modifications that alter the function of TPL or other TPX proteins. Recent examination of the AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) proteins suggests that differential affinity for TPXs can set the threshold for auxin sensitivity and subsequent growth dynamics. Beyond well-established roles in development, the TPX family has also emerged as a hub in plant immunity with effectors from diverse pathogens directly targeting TPX proteins. In one particularly striking case, a species of insect reduces the fitness of its competitors by manipulating TPX activity in the shared host plant to increase a selective suite of plant defenses. The subtle and effective reprogramming of critical developmental and immunity networks via modification of the pool of available TPX proteins could guide engineering strategies to optimize growth-defense trade-offs in crops. In this review, we will summarize recent studies highlighting how modifying the available pool of TPX family members results in subtle and effective reprogramming of critical developmental and immunity networks, and how this mode of regulation could provide a blueprint for optimizing growth-defense trade-offs in crops.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70185"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0