Physiologia plantarum最新文献_第7页

Integrating physiological, metabolome and transcriptome revealed the response of maize seeds to combined cold and high soil moisture stresses.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70096

Xiangzeng Meng, Yujun Cao, Yanjie Lv, Lichun Wang, Yongjun Wang

{"title":"Integrating physiological, metabolome and transcriptome revealed the response of maize seeds to combined cold and high soil moisture stresses.","authors":"Xiangzeng Meng, Yujun Cao, Yanjie Lv, Lichun Wang, Yongjun Wang","doi":"10.1111/ppl.70096","DOIUrl":"https://doi.org/10.1111/ppl.70096","url":null,"abstract":"Combined cold and high moisture stress (CHS) is a prevalent abiotic stress during maize sowing in northeast China, severely affecting the growth of seedlings and seed germination. However, the mechanism underlying seed growth responses to CHS remains unclear. We used Jidan441 (JD441, CHS-resistant) and Jidan558 (JD558, CHS-sensitive) as experimental materials. Treatments of 5-day cold (4°C, CS), high moisture (25%, gravimetric water content, HH), and CHS were initiated at sowing, followed by a return to normal growth conditions (20°C during light/ 15°C during dark, 15%) at 7 days after sowing (DAS). CS, HH, and CHS decreased seed root length and surface area. The reduction in root length and surface area in JD441 due to CHS was less severe than in JD558. We found that the difference between CHS and control in JD441was less than that in JD558 at transcriptional and metabolic levels at 7 DAS. After CHS removal, JD441 exhibited a greater increase in α-amylase activity and antioxidant content than JD558, which facilitated starch decomposition and the rapid removal of O2 - and H2O2 in seeds. The rapid recovery of soluble sugar and soluble protein in JD441 helped maintain osmotic balance. Amino acids and genes related to amino acid metabolism were upregulated in response to combined stress in JD441, whereas they were downregulated in JD558. In conclusion, the stress tolerance of JD441 was attributed to its efficient recovery ability from CHS. This study provides a scientific foundation for exploring seed stress tolerance pathways and developing cold and high-moisture-tolerant hybrids.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70096"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoperiodic flowering and AFT/FTL3 gene expression in flowering-time varieties in chrysanthemum.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70086

Shigekazu Takahashi, Yoshihiro Nakano, Katsuhiko Sumitomo, Tamotsu Hisamatsu, Atsushi Oda, Noriyuki Onoue, Tatsumi Mizubayashi, Hiromoto Yamakawa

{"title":"Photoperiodic flowering and AFT/FTL3 gene expression in flowering-time varieties in chrysanthemum.","authors":"Shigekazu Takahashi, Yoshihiro Nakano, Katsuhiko Sumitomo, Tamotsu Hisamatsu, Atsushi Oda, Noriyuki Onoue, Tatsumi Mizubayashi, Hiromoto Yamakawa","doi":"10.1111/ppl.70086","DOIUrl":"https://doi.org/10.1111/ppl.70086","url":null,"abstract":"Chrysanthemum (Chrysanthemum morifolium Ramat.) is a short-day plant, and flowering is stimulated when the photoperiod is shorter than a variety-specific threshold (critical day length). In Japan, summer-to-autumn-flowering cultivars (SA-cvs.) flower from July to September. Little research has been conducted to understand why SA-cvs. bloom earlier than autumn-flowering cultivars (A-cvs.). We conducted a comparative study of the relationship between the photoperiodic response of flowering and the gene expression of florigen FLOWERING LOCUS T-like 3 (FTL3) and antiflorigen anti-florigenic FT/TFL1 (AFT). SA-cvs. had a longer critical day length than A-cvs. However, in both groups, a decrease in AFT and increase in FTL3 were consistently observed below the critical day length when flowering was promoted. The opposite responses (less flowering, low FTL3, and high AFT) were observed for longer than the critical day lengths. This indicated that flowering in SA-cvs. was controlled by the regulation of AFT/FTL3 expression, similar to that in A-cvs. Next, we studied the mechanism that causes a variation in critical day lengths. In SA-cvs., the photosensitive phase, which occurs at night, occurs earlier than that in A-cvs. This indicates a variation in the endogenous time-keeping mechanism. This was supported by the fact that the circadian rhythmicity of leaf movement was weaker in SA-cvs. than that in A-cvs. Thus, variation in the endogenous time-keeping mechanism may cause a longer critical day length and earlier flowering time in SA-cvs.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70086"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Chromosome-Level Genome Sequence Reveals Regulation of Salt Stress Response in Mesembryanthemum crystallinum.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70057

Koichi Toyokura, Ken Naito, Koyuki Makabe, Mami Nampei, Hiroki Natsume, Jutarou Fukazawa, Makoto Kusaba, Akihiro Ueda

{"title":"A Chromosome-Level Genome Sequence Reveals Regulation of Salt Stress Response in Mesembryanthemum crystallinum.","authors":"Koichi Toyokura, Ken Naito, Koyuki Makabe, Mami Nampei, Hiroki Natsume, Jutarou Fukazawa, Makoto Kusaba, Akihiro Ueda","doi":"10.1111/ppl.70057","DOIUrl":"https://doi.org/10.1111/ppl.70057","url":null,"abstract":"Salt stress disturbs plant growth and photosynthesis due to its toxicity. The ice plant Mesembryanthemum crystallinum is a highly salt-tolerant facultative crassulacean acid metabolism (CAM) plant. However, the genetic basis of the salt tolerance mechanisms in ice plants remains unclear. In this study, we constructed a chromosome-level whole-genome sequence of the ice plant and performed transcriptome analysis of the effects of salt treatment on the leaves. After 24-hour 500 mM NaCl treatment to the roots, 1100 and 1394 genes, including CAM pathway, glycolysis, and inositol metabolism, were up- and down-regulated in the leaves, respectively. Salt treatment also influenced the abscisic acid (ABA) signaling components, including genes from the PYRABACTIN RESISTANCE1 (PYR1) family and the PROTEIN PHOSPHATASE 2CA (PP2CA) family. We detected the induction of the genes encoding various ion transporters after salt treatment. The expression of most v-ATPase subunits is induced, leading to vacuolar acidification, which facilitates sodium ion sequestration in the vacuoles. Additionally, some genes encoding metal ion transporters, including the genes from the ZIP family and NRAMP family, were induced by salt treatment, accompanied by the accumulation of iron, zinc, and copper ions in the leaves. Cis-motif enrichment analysis revealed that ABRE-like motifs and MYB-binding-like motifs were enriched in the upstream sequences of genes that were either up-regulated or down-regulated by salt. In conclusion, this study highlights how salt treatment induces drastic and rapid transcriptomic changes and unveils the ice plant's genomic foundation. Our resources provide further insights into the regulation of salt tolerance in the ice plants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70057"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical, photosynthetic and metabolomics insights of single and combined effects of salinity, heat, cold and drought in Arabidopsis. 盐度、热、冷和干旱对拟南芥的单一和联合效应的生化、光合和代谢组学研究。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70062

Elena Secomandi, Marco Armando De Gregorio, Alejandro Castro-Cegrí, Luigi Lucini

{"title":"Biochemical, photosynthetic and metabolomics insights of single and combined effects of salinity, heat, cold and drought in Arabidopsis.","authors":"Elena Secomandi, Marco Armando De Gregorio, Alejandro Castro-Cegrí, Luigi Lucini","doi":"10.1111/ppl.70062","DOIUrl":"10.1111/ppl.70062","url":null,"abstract":"Ensuring food security is one of the main challenges related to a growing global population under climate change conditions. The increasing soil salinity levels, drought, heatwaves, and late chilling severely threaten crops and often co-occur in field conditions. This work aims to provide deeper insight into the impact of single vs. combined abiotic stresses at the growth, biochemical and photosynthetic levels in Arabidopsis thaliana (L.). Reduced QY max was recorded in salinity-stressed plants, NPQ increased in heat and salinity single and combined stresses, and qP decreased in combined stresses. MDA and H2O2 content were consistently altered under all stress conditions, but higher values were recorded under salinity alone and in combination. Salinity alone and in stress combinations (especially with cold) provided a stronger hierarchical effect. Despite glycine and GABA osmolytes not significantly changing, proline highlighted the hierarchically stronger impact of salinity, while glycine-betaine was decreased under drought combinations. Untargeted metabolomics pointed out distinct metabolic reprogramming triggered by the different stress conditions, alone or in combination. Pathway analysis revealed that abiotic stresses significantly affected hormones, amino acids and derivates, and secondary metabolites. Flavonoids accumulated under drought (alone and combined with heat and cold stresses), while N-containing compounds decreased under all combined stresses. Looking at the interactions across the parameters investigated, antagonistic, additive, or synergistic effects could be observed depending on the biochemical process considered. Notwithstanding, these results contribute to delving into the impact of various stress combinations, hierarchically highlighting the stress-specific effects and pointing out different combinations.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70062"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of native (Quercus robur L.) and alien (Quercus rubra L.) species to water stress and nutrient input in European temperate ecosystems. 欧洲温带生态系统中本地和外来树种对水分胁迫和养分输入的响应

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70070

Morena Rolando, Paola Ganugi, Francesca Secchi, Daniel Said-Pullicino, Eleonora Bonifacio, Luisella Celi

{"title":"Response of native (Quercus robur L.) and alien (Quercus rubra L.) species to water stress and nutrient input in European temperate ecosystems.","authors":"Morena Rolando, Paola Ganugi, Francesca Secchi, Daniel Said-Pullicino, Eleonora Bonifacio, Luisella Celi","doi":"10.1111/ppl.70070","DOIUrl":"10.1111/ppl.70070","url":null,"abstract":"Drought and nutrient-poor soils can increase the invasive potential of non-native species, further changing the ecosystems they invade. The high adaptability of these alien species, especially in their efficient use of resources, improves their resilience against abiotic stress. Here, we evaluated the response of the North American Quercus rubra L. (RO) and the European Quercus robur L. (EO) oak species to drought and nutrient scarcity as single and combined factors. Both species were grown under well-watered or alternating short dry-wet phases, with or without the addition of phosphorous (P) and labelled nitrogen (N). Leaf gas exchanges and stem water potential were measured; moreover, leaf chemical characterization was carried out. Under concurrent low fertility and drought, both species reduced gas exchanges and stem water potential, although RO recovery was faster than EO. Nutrient inputs did not modulate RO's physiological response; however, P supply increased its uptake of the more available N forms (15NH4 15NO3). The different leaf contents of N and P demonstrated that EO has lower nutrient use efficiency compared to RO. Nevertheless, P addition significantly mitigated the drought effects on EO, highlighting the crucial role of this nutrient in aiding EO's recovery under stress conditions. RO invasive potential may be linked to its superior adaptability and resource-use efficiency under combined abiotic stress. Nevertheless, EO competitiveness can be improved through targeted nutrient management.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70070"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterotrimeric G protein interacts with MLO1 to regulate a trade-off between disease resistance and growth in wheat.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70100

Yunwei Zhang, Ziyi Yang, Wanqing Bai, Shuxian Huang, Yaoyu Chen, Jiaqiang Sun

引用次数: 0

The Plant Ionome as a Functional Trait: Variation across Bioclimatic Regions and Functional Groups. 植物离合素作为一种功能性状：跨生物气候区和功能群的变异。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70076

Ofir Katz, Renan Fernandes Moura, Michal Gruntman, Marcelo Sternberg

{"title":"The Plant Ionome as a Functional Trait: Variation across Bioclimatic Regions and Functional Groups.","authors":"Ofir Katz, Renan Fernandes Moura, Michal Gruntman, Marcelo Sternberg","doi":"10.1111/ppl.70076","DOIUrl":"https://doi.org/10.1111/ppl.70076","url":null,"abstract":"Plant chemical composition is a trait gaining increasing importance in plant ecology. However, there is limited research on the patterns and drivers of its variation among different plant functional groups and bioclimatic regions. We conducted an analysis of ionomes utilising X-ray fluorescence on 83 plant species from four distinct functional groups (grasses, legumes, forbs and woody species); we marked plots across 15 sites located in both the desert and Mediterranean bioclimatic regions. The primary factors influencing variations in ionomes are predominantly attributed to bioclimatic factors rather than soil composition. Across all functional groups, plants from the Mediterranean region are characterised by greater association with calcium, whereas desert plants exhibit a higher affinity for strontium (Sr), suggesting its potential role in drought tolerance. Among functional groups, grasses uniquely exhibit distinct ionomic features, primarily due to their higher silicon (Si) concentrations. Plant species' affinities for certain elements and their interactions are likely driven by physiological constraints, whereas variations within a functional group are mostly driven by environmental conditions. We conclude that interactions among elements form physiological phenotypes shaped by natural selection under large-scale environmental variability, making plant ionome composition an important plant functional trait.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70076"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofeedback control of photosynthetic lighting using real-time monitoring of leaf chlorophyll fluorescence. 利用叶片叶绿素荧光实时监测进行光合照明的生物反馈控制。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70073

Suyun Nam, Marc W van Iersel, Rhuanito Soranz Ferrarezi

{"title":"Biofeedback control of photosynthetic lighting using real-time monitoring of leaf chlorophyll fluorescence.","authors":"Suyun Nam, Marc W van Iersel, Rhuanito Soranz Ferrarezi","doi":"10.1111/ppl.70073","DOIUrl":"10.1111/ppl.70073","url":null,"abstract":"Optimizing photosynthetic lighting is essential for maximizing crop production and minimizing electricity costs in controlled environment agriculture (CEA). Traditional lighting methods often neglect the impact of environmental factors, crop type, and light acclimation on photosynthetic efficiency. To address this, a chlorophyll fluorescence-based biofeedback system was developed to adjust light-emitting diode (LED) intensity based on real-time plant responses, rather than using a fixed photosynthetic photon flux density (PPFD). This study used the biofeedback system to maintain a range of target quantum yield of photosystem II (ΦPSII) and electron transport rate (ETR) values and to examine if the adjustment logic (ΦPSII or ETR-based) and crop type influence LED light intensity. The system was tested in a growth chamber with lettuce (Lactuca sativa) 'Green Towers' and cucumber (Cucumis sativus) 'Diva' to maintain six ETR levels (30, 50, 70, 90, 110, 130 μmol·m-2·s-1) and five ΦPSII levels (0.65, 0.675, 0.7, 0.725, 0.75) during a 16-hour photoperiod. The ETR-based biofeedback quickly stabilized the target ETR within 30-45 minutes, whereas the ΦPSII-based system needed more time. The system adjusted light intensities according to target values, acclimation status, and crop-specific responses. For example, to maintain a target ETR of 130 μmol·m-2·s-1, the gradual increase in ΦPSII over time due to light acclimation allowed the required PPFD to decrease by 35 μmol·m-2·s-1. Lettuce showed higher photosynthetic efficiency and lower heat dissipation than cucumber, leading to higher PPFD adjustments for lettuce. This biofeedback system effectively controls LED light, optimizing photosynthetic efficiency and potentially reducing lighting costs.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70073"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal regulation and physiological adjustments of wheat and pea plants under simulated lunar soil conditions.

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70097

Rodrigo A Contreras, Marisol Pizarro, Gustavo E Zúñiga, Cristian Valenzuela

{"title":"Hormonal regulation and physiological adjustments of wheat and pea plants under simulated lunar soil conditions.","authors":"Rodrigo A Contreras, Marisol Pizarro, Gustavo E Zúñiga, Cristian Valenzuela","doi":"10.1111/ppl.70097","DOIUrl":"https://doi.org/10.1111/ppl.70097","url":null,"abstract":"This study investigates the physiological and morphological responses of wheat (Triticum aestivum) and pea (Pisum sativum) grown in a mixture of lunar soil (LS) simulant and organic soil (OS). The experiment compared the growth of both pea and wheat in 100% organic soil (OS) and a 3:2 mixture of OS and LS (OS: LS). Wheat exhibited increased branching and root growth in OS: LS, while pea plants showed enhanced aerial elongation and altered branch morphology. Photochemical efficiency (Fv/Fm) and pigment concentrations were significantly affected, with both pea and wheat showing reduced chlorophyll content in OS: LS. Oxidative stress indicators, such as lipid peroxidation, exhibited higher levels in pea plants than wheat plants, particularly in the OS: LS mixture. Hormonal analysis performed by LC-MS/MS indicated significant increases in abscisic acid (ABA) and its catabolites in both pea and wheat in OS: LS, suggesting an adaptive response to suboptimal conditions. The results highlight species-specific growth strategies, with wheat investing more in root development and pea plants promoting aerial growth. These findings provide important insights into how essential crops could adapt to extraterrestrial soils, contributing to the development of sustainable agricultural practices for space exploration. Future research should focus on optimising crop performance based on species-specific adaptative responses in mixed-soil environments.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70097"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The HECT ubiquitin-protein ligases UPL1 and UPL2 are involved in degradation of Arabidopsis thaliana ACC synthase 7. HECT泛素蛋白连接酶UPL1和UPL2参与拟南芥ACC合成酶7的降解。

IF 5.4 2区生物学

Physiologia plantarum Pub Date : 2025-01-01 DOI: 10.1111/ppl.70030

Małgorzata Marczak, Agata Cieśla, Maciej Janicki, Syed Muhammad Muntazir Mehdi, Piotr Kubiak, Agnieszka Ludwików

{"title":"The HECT ubiquitin-protein ligases UPL1 and UPL2 are involved in degradation of Arabidopsis thaliana ACC synthase 7.","authors":"Małgorzata Marczak, Agata Cieśla, Maciej Janicki, Syed Muhammad Muntazir Mehdi, Piotr Kubiak, Agnieszka Ludwików","doi":"10.1111/ppl.70030","DOIUrl":"https://doi.org/10.1111/ppl.70030","url":null,"abstract":"Ethylene is an important plant hormone whose production relies on the action of key enzymes, one of which is 1-aminocyclopropane-1-carboxylate synthase (ACS). There are three classes of ACS, which are all partially regulated by degradation through the ubiquitin-proteasome system (UPS), which regulates ethylene production. Arabidopsis has a single class III ACS, ACS7, but although it is known to be degraded by the 26S proteasome, the UPS proteins involved are poorly characterised. In this work, we used mass spectrometry to identify novel components of the ubiquitin system that may contribute to the regulation of ethylene biosynthesis via ACS7. We found two HECT-type ligases, UPL1 and UPL2, which regulate ACS7 stability. In vitro experiments showed that UPL1 and UPL2 E3 ligases directly control ACS7 turnover. In addition, increased ethylene levels were observed in UPL1- and UPL2-knockout plants in response to NaCl and NaCl+MG132 treatment, respectively. Under the same conditions, we observed increased ACS7 transcript levels in upl1 compared to WT plants under control and stress conditions, further confirming that UPL1 and UPL2 regulate ACS7-dependent ethylene production in response to stress. We used molecular modelling to predict ACS7 ubiquitylation sites and cell-free degradation assays to verify that lysine residues at positions 174, 238 and 384 regulate ACS7 protein stability. Overall, this study provides new insights into the regulation of ACS7 protein stability, and hence ethylene production, in plant growth and development and the response to stress.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70030"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0