Physiologia plantarum最新文献_第7页

Phytophthora infestans Effector Protein Pi08174 Suppresses Potato Immune Defense via Multiple Resistance Pathways. 马铃薯疫霉效应蛋白Pi08174通过多种抗性途径抑制马铃薯免疫防御

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70561

Jiayi Zheng, Hongjun Li, Weilei Sun, Zewei Zhang, Youjiao Shan, Jun Hu, Xiaolan Zhang, Yanfeng Duan, Guangcun Li

{"title":"Phytophthora infestans Effector Protein Pi08174 Suppresses Potato Immune Defense via Multiple Resistance Pathways.","authors":"Jiayi Zheng, Hongjun Li, Weilei Sun, Zewei Zhang, Youjiao Shan, Jun Hu, Xiaolan Zhang, Yanfeng Duan, Guangcun Li","doi":"10.1111/ppl.70561","DOIUrl":"https://doi.org/10.1111/ppl.70561","url":null,"abstract":"Late blight, caused by Phytophthora infestans, is a devastating disease that affects potato production. During the interactions with P. infestans, effectors are secreted into the plant to control the cellular activity of the host and suppress the plant immune response. However, the pathogenic mechanisms underlying most P. infestans effectors are still unclear. In this study, we focused on effector Pi08174, which is associated with immunosuppression. Agrobacterium-mediated transient expression of Pi08174 induces intense cell death and reactive oxygen species (ROS) accumulation in Nicotiana benthamiana. Overexpression of Pi08174 in potato promotes P. infestans infestation, and the transcriptome analysis of P. infestans inoculated and non-inoculated OE-Pi08174 showed that Pi08174 induced differential expression of genes in the PAMP-triggered immunity (PTI), ethylene (ET) and jasmonic acid (JA) pathways in potatoes when not inoculated with the pathogen. Also, chloroplast, mitochondrial, and antioxidant-related genes are involved in the cellular homeostasis in OE-Pi08174. After inoculation with the pathogen, multiple pathways, including the endoplasmic reticulum and cell wall, were involved in OE-Pi08174 interaction with P. infestans, and Pi08174 increased the expression of genes related to mycelial growth and virulence of P. infestans. These findings provide new insights into the regulation of plant immunity by Pi08174 and deepen our understanding of the complex relationships between pathogens and host plants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70561"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Common Bean miR172c microRNA, a Relevant Regulator of the N-Fixing Symbiosis, Is Activated by SPL and AGL/MADS-Domain Transcription Factors. 普通豆类miR172c microRNA是固定n共生的相关调控因子，可被SPL和AGL/ mads结构域转录因子激活。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70566

Litzy Ayra, Gladys Jiménez-Nopala, Carlos de la Rosa, Sara Isabel Fuentes, Mario Ramírez, Alfonso Leija, Georgina Hernández

{"title":"The Common Bean miR172c microRNA, a Relevant Regulator of the N-Fixing Symbiosis, Is Activated by SPL and AGL/MADS-Domain Transcription Factors.","authors":"Litzy Ayra, Gladys Jiménez-Nopala, Carlos de la Rosa, Sara Isabel Fuentes, Mario Ramírez, Alfonso Leija, Georgina Hernández","doi":"10.1111/ppl.70566","DOIUrl":"10.1111/ppl.70566","url":null,"abstract":"Nitrogen-fixing symbiosis (NFS) between rhizobia and legume plants is a complex and tightly regulated process. Modules of microRNAs (miRNAs) and their targets from different legumes, such as miR156-SPL and miR172-AP2-1, form part of complex cascades relevant for the regulation of NFS. Based on our previous analysis of the regulatory role of the common bean (Phaseolus vulgaris) miR172c-AP2-1 module, as well as in reports from other legumes about the transcriptional regulation of MIR172 in the NFS, in this work, we demonstrated that PvMIR172c is transcriptionally activated by PvSPL9 and PvFUL-like transcription factors from the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE and AGL (AGAMOUS-LIKE/MADS domain) families, respectively. Bioinformatic analysis of the PvMIR172c gene promoter revealed statistically enriched binding sites for both SPL and AGL transcription factors. The PvSPL9 and PvFUL-like genes are highly expressed in roots/nodules from inoculated plants as compared to roots from fertilized plants. Effector/reporter assays in Nicotiana benthamiana leaves transiently transformed with fluorescent reporter constructs confirmed that PvSPL9d and PvFUL-like activate PvMIR172c expression. In addition, we showed an increased level of miR172c in common bean transgenic roots and nodules overexpressing PvSPL9d or PvFUL-like, while the opposite results held in silenced transgenic tissues. These findings provide an additional understanding of the complex regulatory network controlling NFS in common bean plants.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70566"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Omics Integration Reveals Hormonal Adjustments in Irrigated and Nonirrigated Rice Varieties Upon Mythimna separata Feeding. 组学整合揭示灌溉和非灌溉水稻品种在单独饲养时的激素调节。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70464

Guang Wang, Xu Zhang, Xinling Lai, Baoyun Yang, Yanyan He, Zhengfei Li, Yuejin Peng, Bin Chen, Guangzu Du

{"title":"Omics Integration Reveals Hormonal Adjustments in Irrigated and Nonirrigated Rice Varieties Upon Mythimna separata Feeding.","authors":"Guang Wang, Xu Zhang, Xinling Lai, Baoyun Yang, Yanyan He, Zhengfei Li, Yuejin Peng, Bin Chen, Guangzu Du","doi":"10.1111/ppl.70464","DOIUrl":"https://doi.org/10.1111/ppl.70464","url":null,"abstract":"Freshwater consumption is the biggest challenge in irrigated rice cultivation (Oryza sativa L.). Nonirrigated farmland rice cultivation is a strategy to mitigate the water resource shortage associated with traditional irrigated rice farming. Both nonirrigated farmland and irrigated rice face challenges from insect pest attacks, yet the comparative responses of rice grown under these two cultivation methods to insect herbivory have not been extensively studied. In this study, we observed that the population density of the oriental armyworm (Mythimna separata) was higher in nonirrigated farmland rice compared to irrigated rice. To investigate the molecular and metabolic responses of rice to insect feeding, we conducted integrated transcriptomic and metabolomic analyses following 48 h of continuous herbivory by oriental armyworm larvae. Our results showed that insect feeding induced a more pronounced change in the number of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) in irrigated rice than in nonirrigated farmland rice. These DEGs and DAMs were significantly enriched in plant hormone signal transduction pathways. In-depth analysis revealed that the abscisic acid and salicylic acid signaling pathways and the auxin biosynthesis pathway were more responsive to oriental armyworm feeding in irrigated rice compared to nonirrigated farmland rice. Additionally, jasmonic acid biosynthesis and signaling pathways were activated in both irrigated and nonirrigated farmland rice under herbivory stress. In summary, this study provides evidence that nonirrigated farmland rice exhibits a reduced response to insect feeding compared to irrigated rice. These findings contribute to nonirrigated farmland rice resistance or tolerance research and potential applications in pest management.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70464"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RGA1-Mediated Crosstalk of Hormonal, Metabolic, and Redox Networks Sustains Anther Fertility Under Drought Stress in Rice. 干旱胁迫下rga1介导的激素、代谢和氧化还原网络串扰维持水稻花药育性

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70491

Wenhui Yan, Wenfei Hu, Feitong Li, Huanran Wang, Wenting Wang, Yuxiang Zeng, Jiang Hu, Zhihai Wu, Guanfu Fu, Tingting Chen

{"title":"RGA1-Mediated Crosstalk of Hormonal, Metabolic, and Redox Networks Sustains Anther Fertility Under Drought Stress in Rice.","authors":"Wenhui Yan, Wenfei Hu, Feitong Li, Huanran Wang, Wenting Wang, Yuxiang Zeng, Jiang Hu, Zhihai Wu, Guanfu Fu, Tingting Chen","doi":"10.1111/ppl.70491","DOIUrl":"https://doi.org/10.1111/ppl.70491","url":null,"abstract":"The Gα subunit RGA1, a crucial component of heterotrimeric G proteins, has been well-documented to enhance drought resistance in rice seedlings. However, its role during the reproductive stages has remained unexplored. This study aimed to investigate the function of RGA1 in mitigating drought-induced defects in anther and pollen development during pollen mother cell meiosis with Zhonghua 11 (WT), a Gα-deficient mutant (d1), and an RGA1-overexpressing line (OE-1). Under severe drought stress, the three genotypes exhibited significantly decreased spikelet fertility, kernel weight, and grain yield. Concurrently, decreased pollen viability, photosynthetic efficiency, and plant water content were observed, while levels of hydrogen peroxide and malondialdehyde were elevated. Notably, the d1 mutants showed the strongest drought resistance by exhibiting the least physiological disturbances, outperforming both the WT and OE-1 lines. Compared with the d1 mutant, the anthers of drought-stressed WT and OE-1 lines showed significantly more pronounced decreases in carbohydrate contents, ATP levels, ATPase activity, energy charge, and indole acetic acid (IAA) levels. These results demonstrate that the deficiency of RGA1 enhances carbohydrate and energy metabolism, as well as the IAA levels, in anther tissues under drought stress. This enhancement leads to an improvement in the antioxidant capacity of rice plants to suppress the accumulation of peroxides, which ultimately alleviates drought-induced pollen sterility. Findings of this study indicate that RGA1 modulates drought resilience by coordinating hormones, sugars, and energy metabolism.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70491"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Expanded LYR Motif-Containing Protein Family in Archaeplastida. 古塑菌LYR基序蛋白家族的扩展研究。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70482

Etienne H Meyer, Alicia Lopez-Lopez, Olivier Keech, Nicolas Rouhier

{"title":"The Expanded LYR Motif-Containing Protein Family in Archaeplastida.","authors":"Etienne H Meyer, Alicia Lopez-Lopez, Olivier Keech, Nicolas Rouhier","doi":"10.1111/ppl.70482","DOIUrl":"10.1111/ppl.70482","url":null,"abstract":"The LYR motif (LYRM)-containing proteins are small eukaryote-specific proteins that have been defined based on the presence of a Lys-Tyr-Arg amino acid motif and a conserved triplet of α-helices. Twelve LYRM proteins were described in humans. They are involved in core mitochondrial processes as subunits or assembly/stabilising factors of mitochondrial complexes. Their function depends on their ability to interact with the acylated form of acyl-carrier proteins (mtACPs), which places these proteins as direct contributors to two intertwined functional processes, energy metabolism and mitochondrial biogenesis. To gain insight into LYRM proteins in Archaeplastida, we first analyzed the Arabidopsis thaliana genome and then a set of organisms representing the different groups of the Archaeplastida clade. This analysis revealed the existence of 17 classes encompassing 10 of the 12 LYRM classes found in humans. Eleven classes exist in Arabidopsis, and six additional classes are present in some organisms but not in Arabidopsis, thus expanding previous observations. Subsequent data mining based on literature, gene expression, and in silico analyses allowed us to speculate about the possible molecular function of some currently uncharacterised LYRMs in plants. Altogether, this study revealed the diversification of the LYRM protein family in Archaeplastida and more globally among eukaryotes, in which the LYRM-mtACP associations represent central molecular systems to regulate mitochondrial biogenesis upon fluctuating growth conditions.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70482"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"The Last of Them": Entomopathogenic Effect of Akanthomyces muscarius on the Scale Insect Pest Toumeyella parvicornis Under Laboratory Conditions, a Potential Biological Control Candidate. “最后的他们”：在实验室条件下，赤色菌对小角图梅耶氏鳞状害虫的昆虫致病作用，一种潜在的生物防治候选物。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70533

Nicolò Di Sora, Silvia Turco, Federico Brugneti, Flavia Isoli, Angelo Mazzaglia, Mario Contarini, Stefano Speranza, Luca Rossini

{"title":"\"The Last of Them\": Entomopathogenic Effect of Akanthomyces muscarius on the Scale Insect Pest Toumeyella parvicornis Under Laboratory Conditions, a Potential Biological Control Candidate.","authors":"Nicolò Di Sora, Silvia Turco, Federico Brugneti, Flavia Isoli, Angelo Mazzaglia, Mario Contarini, Stefano Speranza, Luca Rossini","doi":"10.1111/ppl.70533","DOIUrl":"10.1111/ppl.70533","url":null,"abstract":"Entomopathogenic fungi are valuable alternatives to traditional agrochemicals, providing more sustainable crop protection. Akanthomyces spp. is acknowledged in biocontrol practices, especially in controlling aphids, thrips, whiteflies, and mites; however, its efficacy on soft scale insects is still poorly known. This study investigates the potential use of Akanthomyces muscarius on the soft scale insect Toumeyella parvicornis, a new invasive pest for European stone pines (Pinus pinea). The strain of A. muscarius tested in the experimentation has been isolated from Parthenolecanium corni, a soft scale insect on which the fungus seems to be highly infective. After molecular identification and characterization, bioassays were conducted to compare the performances of the different conidial concentrations of A. muscarius isolate and commercial formulations on T. parvicornis overwintering females and nymphs. Results showed a promising colonization and higher pathogenicity of A. muscarius on T. parvicornis in all the trials, compared with the other commercial formulations, in some cases showing a quick infection and death of the host. Our results pave the way to further field uses where A. muscarius is applied as a biological control method to reduce infestations of T. parvicornis.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70533"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12449707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Consecutive Moderate and Severe Drought Stresses Affect Chlorophyll Fluorescence and Non-structural Carbohydrates Dynamics in Grapevine Leaves. 连续中重度干旱胁迫对葡萄叶片叶绿素荧光和非结构性碳水化合物动态的影响

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70535

Monica Canton, Francesco Mirone, Franco Meggio, Alessandro Pichierri, Valentino Casolo, Giovanni Battista Tornielli, Andrea Pitacco

{"title":"Consecutive Moderate and Severe Drought Stresses Affect Chlorophyll Fluorescence and Non-structural Carbohydrates Dynamics in Grapevine Leaves.","authors":"Monica Canton, Francesco Mirone, Franco Meggio, Alessandro Pichierri, Valentino Casolo, Giovanni Battista Tornielli, Andrea Pitacco","doi":"10.1111/ppl.70535","DOIUrl":"10.1111/ppl.70535","url":null,"abstract":"Drought events represent a growing challenge for agriculture in the Mediterranean region, particularly for Vitis vinifera, a species with economic and cultural significance. This study evaluates the effects of two subsequent drought stress events on grapevine cv. Sauvignon blanc, combining physiological and biochemical approaches. The trial was conducted in a semi-controlled tunnel from mid-June to mid-July 2024 on potted vines. Plants were divided into well-watered and drought groups. Drought-stressed vines underwent two drought cycles: the first reaching -1.3 MPa stem water potential, followed by rewatering, and the second with either moderate (-1.3 MPa) or severe (-2.5 MPa) drought before another rewatering. Stomatal conductance, chlorophyll fluorescence, gas exchange, and nonstructural carbohydrate levels were measured to assess physiological responses. Grape bunches were analyzed for C13/C12 isotope ratios at harvest. Significant physiological and biochemical differences were observed between the first moderate drought and both the second moderate and severe drought, highlighting distinct plant responses to water stress and rewatering. In the second cycle, vines subjected to severe drought showed significantly reduced photosynthetic efficiency compared to those under moderate drought, suggesting a decline in resilience. Chlorophyll fluorescence data indicated sustained photoinhibition after severe drought, while differences in nonstructural carbohydrate levels between treatments and across different times of the day revealed shifts in carbon metabolism. Carbon isotope composition confirmed the effect of double water stress. These results highlight the grapevines' capacity for physiological adaptation to repeated drought while also indicating the potential accumulation of negative effects if stress becomes excessive or prolonged.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70535"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZmGDI1 Modulates Stomatal Development and Drought Response via the Rho GTPase Pathway Regulation in Maize. ZmGDI1通过Rho GTPase通路调控玉米气孔发育和干旱响应

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70528

Juanjuan Xu, Xiaotong Dong, Kedong Hui, Yuan Liu, Youzhi Li, Xianwei Fan

{"title":"ZmGDI1 Modulates Stomatal Development and Drought Response via the Rho GTPase Pathway Regulation in Maize.","authors":"Juanjuan Xu, Xiaotong Dong, Kedong Hui, Yuan Liu, Youzhi Li, Xianwei Fan","doi":"10.1111/ppl.70528","DOIUrl":"https://doi.org/10.1111/ppl.70528","url":null,"abstract":"Guanine nucleotide dissociation inhibitors (GDIs) regulate cell signaling in mammals, but their role in stomatal development in plants remains unknown. This study investigates the role of the maize GDI1 (ZmGDI1) in stomatal formation and drought stress responses. Heterologous expression of ZmGDI1 in Arabidopsis reduced the transcription of key stomatal development genes including SPEECHLESS (SPCH), FAMA, and MUTE, resulting in reduced stomatal density by 13% compared to wild-type plants. Conversely, atgdi1 loss-of-function mutants exhibited increased stomatal density and upregulation of these master regulators, resulting in enhanced drought sensitivity. The phenotypes observed in the atgdi1 mutant were rescued by the expression of ZmGDI1, suggesting that the function of GDIs in stomatal development is conserved across different plant species. ZmGDI1-silenced maize plants generated via virus-induced gene silencing (VIGS) showed a marked increase in stomatal density, confirming the functional conservation of the ZmGDI1 gene. ZmGDI1 was demonstrated to interact with and inhibit the activity of the Rho GTPase ZmROP4, which subsequently associates with ZmPAN1/2 to promote stomatal development. These findings uncover a conserved GDI-ROP signaling module that governs stomatal patterning and provides molecular targets for engineering improved drought tolerance in crops.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70528"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted Regulation and Functional Versatility of miR164 in Plant: From Molecular Mechanisms to Crop Improvement. miR164在植物中的多面调控和功能多样性：从分子机制到作物改良。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70548

Jie Gao, Lulu Chen, Ni Yuan, Yuxin Liu, Yuqiang Sun, Liping Ke

{"title":"Multifaceted Regulation and Functional Versatility of miR164 in Plant: From Molecular Mechanisms to Crop Improvement.","authors":"Jie Gao, Lulu Chen, Ni Yuan, Yuxin Liu, Yuqiang Sun, Liping Ke","doi":"10.1111/ppl.70548","DOIUrl":"https://doi.org/10.1111/ppl.70548","url":null,"abstract":"MicroRNA164 (miR164), a highly conserved plant-specific miRNA family, serves as a pivotal regulator in diverse aspects of plant growth, development, and stress responses primarily through targeting NAC transcription factors and other downstream genes. This comprehensive review systematically presents the structural characteristics, regulatory mechanisms, and functional diversity of miR164 across various plant species. The evolutionary conservation in mature sequences of miR164 determines functional specificity, with base variations at non-cleavage sites potentially expanding the target repertoire and regulatory scenarios; the species-specific expansion of miR164 members exhibits partial functional redundancy and enables functional diversification. The conserved miR164-NAC regulatory module and newly identified non-NAC targets have been revealed to be involved in plant growth and response to stresses. The precise spatiotemporal expression patterns of miR164, dynamically regulated by specific transcriptional controllers and environmental cues, attract further study. Given its multifunctional roles, miR164 and miR164-TF modules represent a promising target for crop genetic improvement. This review introduces the current research status on miR164 regulatory networks, functional versatility, and validation methodologies, while highlighting its significant potential in developing stress-resilient crops and enhancing agricultural productivity through targeted genetic engineering approaches.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70548"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear Accumulation of OsMADS27 Was Promoted by OsNAR2.1 in Response to Nitrate to Control Root Growth by Directly Targeting OsMADS57 in Rice. 水稻OsMADS27在硝酸盐胁迫下通过直接靶向OsMADS57，促进OsMADS27的核积累，控制根系生长。

IF 3.6 2区生物学

Physiologia plantarum Pub Date : 2025-09-01 DOI: 10.1111/ppl.70553

Junyu Wu, Shuaiqi Yang, Muhammad Rehman, Nana Chen, Bohan Liu, Guohua Xu, Xiaorong Fan, Yinbo Gan

{"title":"Nuclear Accumulation of OsMADS27 Was Promoted by OsNAR2.1 in Response to Nitrate to Control Root Growth by Directly Targeting OsMADS57 in Rice.","authors":"Junyu Wu, Shuaiqi Yang, Muhammad Rehman, Nana Chen, Bohan Liu, Guohua Xu, Xiaorong Fan, Yinbo Gan","doi":"10.1111/ppl.70553","DOIUrl":"https://doi.org/10.1111/ppl.70553","url":null,"abstract":"Nitrate serves not only as an essential nitrogen source but also as a signalling molecule to regulate plant growth and development. However, the mechanisms by which plants respond to nitrate to regulate root growth in rice remain largely unclear. In this study, we demonstrated that the overexpression of OsMADS27 significantly promoted root growth and nitrate absorption in response to nitrate supply, whereas RNA interference (RNAi) lines exhibited the opposite phenotype. OsMADS27 is predominantly expressed in the root, and the mutation of OsNAR2.1 impaired both the response rate and intensity of OsMADS27 under nitrate treatment. Notably, OsMADS27 is co-localised with OsNAR2.1, and the mutation of OsNAR2.1 affected the nuclear localisation of OsMADS27. Yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC) and co-immunoprecipitation (Co-IP) assays confirmed a direct protein interaction between OsMADS27 and OsNAR2.1. More importantly, chromatin immunoprecipitation followed by qPCR (ChIP-qPCR), dual-luciferase (Dual-LUC) and yeast one-hybrid (Y1H) assays demonstrated that OsMADS27 could directly regulate the expression of OsMADS57 to control root growth. Furthermore, OsMADS27 could also promote the expression of several nitrate transporter genes, including OsNRT1.2, OsNRT2.1, OsNRT2.3a and OsNRT2.4, to regulate nitrate absorption in rice. Collectively, this study uncovers a novel pathway in which OsMADS27 promotes root growth in response to nitrate by directly regulating OsMADS57 expression in an OsNAR2.1-dependent manner. These findings will broaden our understanding and provide a novel regulatory network of OsMADS genes mediating root development through nitrate signalling in rice, which also has an important application value for molecular breeding to improve nitrogen use efficiency.","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70553"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0