Integrated Transcriptomic and Proteomic Analyses Demonstrated That MeJA-Regulated VvPAL10 Enhances Cold Tolerance in Grapevines.

Abstract

Understanding the molecular mechanisms underlying cold and methyl jasmonate (MeJA) responses is vital for improving the cold tolerance of grapes. This study treated 'Pinot noir' plantlets with MeJA, screened key genes in the regulatory pathway using transcriptomics and proteomics analyses, and investigated their regulatory mechanisms under cold stress. The results showed that 50 μmol L^-1 MeJA significantly inhibited the growth of grape roots length, increased the endogenous MeJA content and antioxidant enzyme activities, and reduced membrane damage under cold stress. In addition, 50 μmol L^-1 MeJA and cold stress treatment greatly increased the number of differential genes and metabolites in the phenylalanine synthesis and hormone signal transduction pathways. The results indicated that VvPAL10, an important gene in the phenylalanine synthesis pathway, significantly improved transgenic Arabidopsis thaliana and grapevine callus tissue tolerance to low temperatures.