重要环状rna、微小rna和靶基因参与槭根生根过程。

IF 3.6 2区 生物学 Q1 PLANT SCIENCES
Jiayu Yu, Jiaming Qin, Junjie Wang, Kezhong Zhang, Wei Ge
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引用次数: 0

摘要

槭是一种在世界范围内广泛种植的多功能树种,具有极高的商业价值。在自然条件下不易生根,在茎插枝中需要利用植物生长激素促进不定根的形成。本研究旨在确定非编码RNA和mrna是如何在竞争性内源RNA相互作用模式下调控龙骨根生根过程的。通过对照组和处理组(500 mg/L IBA作用30 min)的全转录组分析,我们选择了133个差异表达mrna、58个差异表达mirna、81个差异表达lncrna和3个差异表达circrna。在这些差异表达的mirna中,34个差异表达的mirna可以靶向100个基因。此外,鉴定了2105个circrna,其中145个与生根相关的miR160、miR164和miR171相互作用。最后,我们选择了ciRNA46-miR164b-NAC1调控网络。实时定量聚合酶链反应、双荧光素酶测定和β-葡萄糖醛酸酶基因组织染色实验验证了ciRNA46-miR164b-NAC1之间的相互作用。过表达实验表明,NAC1促进不定根的发育,而miR164b抑制不定根的发育。双分子荧光互补和酵母双杂交显示NAC1与SHORT-ROOT的相互作用。这些结果解释了白杨生根过程中的作用机制,为进一步研究白杨生根过程中的分子机制提供了科学依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Significant circRNAs, microRNAs, and Target Genes Participate in the Rooting Process of Acer truncatum.

Acer truncatum is a multifunctional tree species widely planted worldwide with extremely high commercial value. It is not easy to take root under natural conditions, and plant growth hormone needs to be used to promote the formation of adventitious roots in stem cuttings. This study aimed to determine how non-coding RNAs and mRNAs regulate the rooting process of A. truncatum in the mode of competitive endogenous RNA interactions. Based on whole transcriptome analysis of the control and treatment (500 mg/L IBA for 30 min) groups, 133 differentially expressed mRNAs, 58 differentially expressed miRNAs, 81 differentially expressed lncRNAs, and 3 differentially expressed circRNAs were selected. Among the differentially expressed miRNAs, 34 differentially expressed miRNAs can target 100 genes. Moreover, 2105 circRNAs were identified, of which 145 interacted with rooting-related miR160, miR164, and miR171. Finally, the ciRNA46-miR164b-NAC1 regulatory network was selected. Real-time quantitative polymerase chain reaction, dual luciferase assays, and β-glucuronidase gene tissue staining experiments verified the interaction among ciRNA46-miR164b-NAC1. Overexpression experiments showed that NAC1 promoted the development of adventitious roots, whereas miR164b inhibited their development. Bimolecular fluorescence complementation and yeast two-hybrid revealed the interaction of NAC1 with SHORT-ROOT. These results explain the mechanism of action in the rooting process of A. truncatum, offering a scientific foundation for further research on its molecular mechanisms during rooting.

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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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