A Comprehensive Analysis of Transcriptomics and Metabolomics Revealed LcMYC4 Regulating LcDXS3 to Promote Terpenoid Biosynthesis in Litsea cubeba.

IF 3.6 2区 生物学 Q1 PLANT SCIENCES
Jiahui Yang, Feifei Ni, Yicun Chen, Ming Gao, Yunxiao Zhao, Yangdong Wang
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引用次数: 0

Abstract

Terpenoids are present in many plant species and confer various biological activities. Litsea cubeba, a traditional Chinese medicinal plant, exhibits antibacterial, antioxidant, and anti-tumor properties, primarily due to its terpenoid constituents. As important secondary metabolites, terpenoid biosynthesis is often influenced by hormonal treatments; however, the molecular mechanisms underlying hormone-induced terpenoid synthesis remain unclear. This study elucidates the regulatory mechanisms of terpenoid biosynthesis in L. cubeba under jasmonic acid (JA) treatment through comprehensive transcriptome analysis and gene functional characterization. Notably, terpene content in L. cubeba fruit increased substantially within 6 h following JA treatment. Transcriptome analysis revealed that JA signaling and terpenoid biosynthesis genes were significantly upregulated and co-expressed following JA induction. LcMYC4, a core gene in JA signal transduction, exhibited an expression pattern similar to that of LcDXS3, a key enzyme in the terpenoid precursor synthesis pathway. Overexpression of LcMYC4 significantly enhanced terpenoid accumulation in L. cubeba, and this increase in LcMYC4 expression was accompanied by a significant upregulation of LcDXS3 expression. Besides, LcMYC4 directly binds the LcDXS3 promoter and activates its expression. In summary, this study provides insight into the mechanisms of JA-induced terpenoid synthesis in L. cubeba.

转录组学和代谢组学综合分析揭示LcMYC4调控LcDXS3促进山苍子萜类生物合成。
萜类化合物存在于许多植物物种中,并赋予各种生物活性。山苍子是一种传统的中药植物,主要由于其萜类成分而具有抗菌、抗氧化和抗肿瘤的特性。萜类化合物作为重要的次生代谢物,其生物合成常受激素处理的影响;然而,激素诱导的萜类化合物合成的分子机制尚不清楚。本研究通过综合转录组分析和基因功能表征,阐明茉莉酸(jasmonic acid, JA)处理下L. cubeba萜类生物合成的调控机制。在JA处理后6 h内,猕猴桃果实中萜烯含量显著增加。转录组分析显示,JA诱导后,JA信号和萜类生物合成基因显著上调和共表达。LcMYC4是JA信号转导的核心基因,其表达模式与萜类前体合成途径的关键酶LcDXS3相似。LcMYC4的过表达显著增强了L. cubeba中萜类物质的积累,并且LcMYC4表达的增加伴随着LcDXS3表达的显著上调。此外,LcMYC4直接结合LcDXS3启动子并激活其表达。综上所述,本研究揭示了ja诱导牛蒡萜类化合物合成的机制。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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