生长素梯度对豌豆生殖发育的影响。

IF 3.6 2区 生物学 Q1 PLANT SCIENCES
Dilini D Adihetty, Harleen Kaur, Charitha P A Jayasinghege, Dennis M Reinecke, Jocelyn A Ozga
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引用次数: 0

摘要

生长素参与果实和发育的调控;然而,IAA在豌豆(Pisum sativum)中的作用尚不清楚,因为内源性生长素4-Cl-IAA似乎是刺激子房(果皮)生长的生长素。为了进一步了解生长素在果实发育过程中的作用,我们对该模式豆科植物的生长素定位、定量、转运和基因表达活性进行了评估。果皮维管束组织、花梗和花序梗中的IAA水平和生长素活性(DR5: β-Glucuronidase [GUS]染色和酶活性)在去籽后显著降低,反映了种子通过这些组织产生生长素转运流。去籽改变了果皮和附着组织中生长素反应因子PsARF7/19、PsARF8和PsARF5的转录水平,表明生长素和乙烯对它们的表达有组织特异性调控。在果皮中施用极性生长素运输抑制剂n -1-萘基邻苯二甲酸(NPA)提高了种子果皮中生长素(DR5::GUS染色/酶)活性,但对去籽果没有作用,而在果皮中施用NPA改变了IAA水平和DR5::GUS染色/酶活性,表明生长素从种子向周围组织的极性运输。然而,施用NPA不像其他模式种那样诱导单性果实生长。这些数据表明,在豌豆中,生长素至少部分通过npa敏感途径从种子运输到邻近组织,种子来源的IAA在维持生长素在果皮和附着组织中的梯度中发挥作用,可能使种子成为主要的储存库,生长素和乙烯途径相互作用决定了果实发育的命运。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Auxin Gradients Determine Reproductive Development in Pea (Pisum sativum).

Auxins are involved in the regulation of fruit set and development; however, the role of IAA is unclear in pea (Pisum sativum) since the endogenous auxin 4-Cl-IAA appears to be the auxin stimulating ovary (pericarp) growth. To further understand the role of auxins during fruit development, auxin localization, quantitation, transport, and gene expression activity were assessed in this model legume species. IAA levels and auxin activity (DR5::β-Glucuronidase [GUS] staining and enzyme activity) were substantially reduced in the pericarp vascular tissues, pedicels, and peduncles of fruit upon seed removal, reflecting auxin transport streams derived from the seeds through these tissues. Seed removal modified auxin response factor PsARF7/19, PsARF8, and PsARF5 transcript levels in the pericarp and attachment tissues in a manner suggesting tissue-specific regulation of their expression by auxin and ethylene. Pericarp application of polar auxin transport inhibitor N-1-naphthylphthalamic acid (NPA) increased auxin (DR5::GUS staining/enzyme) activity within pericarps of seeded, but not deseeded fruits, and NPA application to the peduncle modified IAA levels and DR5::GUS staining/enzyme activity, suggesting polar auxin transport from the seeds to surrounding tissues. However, the NPA application did not induce parthenocarpic fruit growth as in other model species. These data support that in pea, auxin is transported from the seeds to adjacent tissues at least partially through NPA-sensitive pathways, that seed-derived IAA plays a role in maintaining auxin gradients through the pericarp and attachment tissues likely for establishing the seed as a major sink, and that auxin and ethylene pathways interact to determine the fate of fruit development.

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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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