Transcriptome Analysis Reveals the Mechanism of Early Branching of Balsa (Ochroma lagopus Swartz).

IF 3.6 2区 生物学 Q1 PLANT SCIENCES
Yiwen Qian, Xue Bai, Ping Huang, Lun Wang, Wanxian Zhu, Yiqing Su, Mingyong Tang
{"title":"Transcriptome Analysis Reveals the Mechanism of Early Branching of Balsa (Ochroma lagopus Swartz).","authors":"Yiwen Qian, Xue Bai, Ping Huang, Lun Wang, Wanxian Zhu, Yiqing Su, Mingyong Tang","doi":"10.1111/ppl.70509","DOIUrl":null,"url":null,"abstract":"<p><p>Balsa (Ochroma lagopus Swartz), the world's lightest wood and a crucial material in wind turbine blades, holds significant potential to contribute to carbon neutrality efforts when cultivated in tropical areas such as Xishuangbanna, China. However, balsa trees planted in Xishuangbanna exhibit early branching, resulting in reduced wood yield. Our study investigated the pivotal factors in regulating shoot apical dominance and branching by comparing an early-branching cultivar from Indonesia with a late-branching cultivar from Ecuador. Through transcriptome analysis, we found that the decreased expression of genes putatively involved in shoot apical meristem (SAM) activity, including putative orthologs of WUSCHEL (OlWUS-like), SHOOTMERISTEMLESS (OlSTM-like), and CLAVATA3 (OlCLV3-like), was associated with shoot apical dominance and lateral meristem development in early-branch cultivars. Additionally, the increased expression of flowering-related genes, including putative orthologs of FLOWERING LOCUS T (OlFT-like), LEAFY (OlLFY-like), and MADS-box genes, was implicated in flowering meristem formation and branch initiation. Notably, we identified a highly expressed OlFT-like gene that may participate in flowering and branching. Overexpression of OlFT-like in Jatropha curcas inhibited the longitudinal growth of the main stem, promoted branching, and intrinsically decreased the expression of genes involved in the control of meristematic activity (JcWUSa, JcWUSb, and JcCLV3). In summary, OlFT-like was identified as a key regulator of flowering and branching in balsa. Our in-depth understanding of the branching of perennials provides novel insights for the early screening of elite ecotypes and molecular breeding of balsa.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70509"},"PeriodicalIF":3.6000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70509","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Balsa (Ochroma lagopus Swartz), the world's lightest wood and a crucial material in wind turbine blades, holds significant potential to contribute to carbon neutrality efforts when cultivated in tropical areas such as Xishuangbanna, China. However, balsa trees planted in Xishuangbanna exhibit early branching, resulting in reduced wood yield. Our study investigated the pivotal factors in regulating shoot apical dominance and branching by comparing an early-branching cultivar from Indonesia with a late-branching cultivar from Ecuador. Through transcriptome analysis, we found that the decreased expression of genes putatively involved in shoot apical meristem (SAM) activity, including putative orthologs of WUSCHEL (OlWUS-like), SHOOTMERISTEMLESS (OlSTM-like), and CLAVATA3 (OlCLV3-like), was associated with shoot apical dominance and lateral meristem development in early-branch cultivars. Additionally, the increased expression of flowering-related genes, including putative orthologs of FLOWERING LOCUS T (OlFT-like), LEAFY (OlLFY-like), and MADS-box genes, was implicated in flowering meristem formation and branch initiation. Notably, we identified a highly expressed OlFT-like gene that may participate in flowering and branching. Overexpression of OlFT-like in Jatropha curcas inhibited the longitudinal growth of the main stem, promoted branching, and intrinsically decreased the expression of genes involved in the control of meristematic activity (JcWUSa, JcWUSb, and JcCLV3). In summary, OlFT-like was identified as a key regulator of flowering and branching in balsa. Our in-depth understanding of the branching of perennials provides novel insights for the early screening of elite ecotypes and molecular breeding of balsa.

Balsa (Ochroma lagopus Swartz)早期分支机制的转录组分析。
巴尔萨(Ochroma lagopus Swartz)是世界上最轻的木材,也是风力涡轮机叶片的重要材料,如果在中国西双版纳等热带地区种植,将为碳中和努力做出巨大贡献。然而,西双版纳种植的巴尔沙树表现出早枝现象,导致木材产量下降。本研究通过对印度尼西亚早分枝品种和厄瓜多尔晚分枝品种的比较,探讨了调节茎尖优势和分枝的关键因素。通过转录组分析,我们发现与茎尖分生组织(SAM)活性相关的基因(包括WUSCHEL (OlWUS-like)、SHOOTMERISTEMLESS (OlSTM-like)和CLAVATA3 (OlCLV3-like))的表达降低与早枝品种茎尖优势和侧边分生组织发育有关。此外,开花相关基因的表达增加,包括开花位点T (OlFT-like)、LEAFY (OlLFY-like)和MADS-box基因的推定同源基因,与开花分生组织的形成和分支的形成有关。值得注意的是,我们发现了一个高表达的olft样基因,可能参与开花和分枝。在麻疯树中,OlFT-like的过表达抑制了主茎的纵向生长,促进了分支,本质上降低了控制分生组织活性的基因(JcWUSa、JcWUSb和JcCLV3)的表达。综上所述,OlFT-like是巴尔沙开花和分枝的关键调控因子。我们对多年生植物分枝的深入了解,为轻木优良生态型的早期筛选和分子育种提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信