Jiahui Yang, Feifei Ni, Yicun Chen, Ming Gao, Yunxiao Zhao, Yangdong Wang
{"title":"转录组学和代谢组学综合分析揭示LcMYC4调控LcDXS3促进山苍子萜类生物合成。","authors":"Jiahui Yang, Feifei Ni, Yicun Chen, Ming Gao, Yunxiao Zhao, Yangdong Wang","doi":"10.1111/ppl.70518","DOIUrl":null,"url":null,"abstract":"<p><p>Terpenoids are present in many plant species and confer various biological activities. Litsea cubeba, a traditional Chinese medicinal plant, exhibits antibacterial, antioxidant, and anti-tumor properties, primarily due to its terpenoid constituents. As important secondary metabolites, terpenoid biosynthesis is often influenced by hormonal treatments; however, the molecular mechanisms underlying hormone-induced terpenoid synthesis remain unclear. This study elucidates the regulatory mechanisms of terpenoid biosynthesis in L. cubeba under jasmonic acid (JA) treatment through comprehensive transcriptome analysis and gene functional characterization. Notably, terpene content in L. cubeba fruit increased substantially within 6 h following JA treatment. Transcriptome analysis revealed that JA signaling and terpenoid biosynthesis genes were significantly upregulated and co-expressed following JA induction. LcMYC4, a core gene in JA signal transduction, exhibited an expression pattern similar to that of LcDXS3, a key enzyme in the terpenoid precursor synthesis pathway. Overexpression of LcMYC4 significantly enhanced terpenoid accumulation in L. cubeba, and this increase in LcMYC4 expression was accompanied by a significant upregulation of LcDXS3 expression. Besides, LcMYC4 directly binds the LcDXS3 promoter and activates its expression. In summary, this study provides insight into the mechanisms of JA-induced terpenoid synthesis in L. cubeba.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70518"},"PeriodicalIF":3.6000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Comprehensive Analysis of Transcriptomics and Metabolomics Revealed LcMYC4 Regulating LcDXS3 to Promote Terpenoid Biosynthesis in Litsea cubeba.\",\"authors\":\"Jiahui Yang, Feifei Ni, Yicun Chen, Ming Gao, Yunxiao Zhao, Yangdong Wang\",\"doi\":\"10.1111/ppl.70518\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Terpenoids are present in many plant species and confer various biological activities. Litsea cubeba, a traditional Chinese medicinal plant, exhibits antibacterial, antioxidant, and anti-tumor properties, primarily due to its terpenoid constituents. As important secondary metabolites, terpenoid biosynthesis is often influenced by hormonal treatments; however, the molecular mechanisms underlying hormone-induced terpenoid synthesis remain unclear. This study elucidates the regulatory mechanisms of terpenoid biosynthesis in L. cubeba under jasmonic acid (JA) treatment through comprehensive transcriptome analysis and gene functional characterization. Notably, terpene content in L. cubeba fruit increased substantially within 6 h following JA treatment. Transcriptome analysis revealed that JA signaling and terpenoid biosynthesis genes were significantly upregulated and co-expressed following JA induction. LcMYC4, a core gene in JA signal transduction, exhibited an expression pattern similar to that of LcDXS3, a key enzyme in the terpenoid precursor synthesis pathway. Overexpression of LcMYC4 significantly enhanced terpenoid accumulation in L. cubeba, and this increase in LcMYC4 expression was accompanied by a significant upregulation of LcDXS3 expression. Besides, LcMYC4 directly binds the LcDXS3 promoter and activates its expression. In summary, this study provides insight into the mechanisms of JA-induced terpenoid synthesis in L. cubeba.</p>\",\"PeriodicalId\":20164,\"journal\":{\"name\":\"Physiologia plantarum\",\"volume\":\"177 5\",\"pages\":\"e70518\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physiologia plantarum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1111/ppl.70518\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70518","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
A Comprehensive Analysis of Transcriptomics and Metabolomics Revealed LcMYC4 Regulating LcDXS3 to Promote Terpenoid Biosynthesis in Litsea cubeba.
Terpenoids are present in many plant species and confer various biological activities. Litsea cubeba, a traditional Chinese medicinal plant, exhibits antibacterial, antioxidant, and anti-tumor properties, primarily due to its terpenoid constituents. As important secondary metabolites, terpenoid biosynthesis is often influenced by hormonal treatments; however, the molecular mechanisms underlying hormone-induced terpenoid synthesis remain unclear. This study elucidates the regulatory mechanisms of terpenoid biosynthesis in L. cubeba under jasmonic acid (JA) treatment through comprehensive transcriptome analysis and gene functional characterization. Notably, terpene content in L. cubeba fruit increased substantially within 6 h following JA treatment. Transcriptome analysis revealed that JA signaling and terpenoid biosynthesis genes were significantly upregulated and co-expressed following JA induction. LcMYC4, a core gene in JA signal transduction, exhibited an expression pattern similar to that of LcDXS3, a key enzyme in the terpenoid precursor synthesis pathway. Overexpression of LcMYC4 significantly enhanced terpenoid accumulation in L. cubeba, and this increase in LcMYC4 expression was accompanied by a significant upregulation of LcDXS3 expression. Besides, LcMYC4 directly binds the LcDXS3 promoter and activates its expression. In summary, this study provides insight into the mechanisms of JA-induced terpenoid synthesis in L. cubeba.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.