Dazhuang Qi, Huirong Xiao, Huihui Liu, Linlin Zheng, Yingchun Wang
{"title":"Sucrose Enhances Adventitious Root Formation in Nitraria tangutorum Under Drought Stress via Hormonal Homeostasis and Hydrogen Peroxide Signaling.","authors":"Dazhuang Qi, Huirong Xiao, Huihui Liu, Linlin Zheng, Yingchun Wang","doi":"10.1111/ppl.70521","DOIUrl":null,"url":null,"abstract":"<p><p>The robust adventitious root (AR) system of Nitraria tangutorum facilitates its adaptation to drought stress; however, the molecular mechanisms governing AR formation during drought remain unclear. In this study, we demonstrated that a sucrose (40 g L<sup>-1</sup>) treatment of N. tangutorum explants for 48 h significantly increased the rate of AR development from 36% in the untreated control group to 100%. Transcriptome analysis revealed sucrose exerts a significant influence on H<sub>2</sub>O<sub>2</sub> homeostasis and hormone signal transduction pathways. Sucrose treatment promoted IAA biosynthesis through the upregulation of TSA, TSB, and YUC genes and suppressed gibberellin (GA) and cytokinin biosynthesis by downregulating CPS, GA3ox, GA20ox, and LOG. Moreover, sucrose induced abscisic acid (ABA) and salicylic acid (SA) accumulation while reducing JA-Ile, and activated H<sub>2</sub>O<sub>2</sub> signaling through the upregulation of RBOH. Exogenous H<sub>2</sub>O<sub>2</sub> (1-8 mM) mimicked the effects of sucrose; however, this effect was abolished by the auxin transport inhibitor TIBA, indicating that the effects of H<sub>2</sub>O<sub>2</sub> depend on auxin signaling. These results reflect that sucrose acts as an energy substrate as well as a signaling molecule to coordinate hormonal interactions and redox signaling during drought-adaptive AR formation.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70521"},"PeriodicalIF":3.6000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70521","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The robust adventitious root (AR) system of Nitraria tangutorum facilitates its adaptation to drought stress; however, the molecular mechanisms governing AR formation during drought remain unclear. In this study, we demonstrated that a sucrose (40 g L-1) treatment of N. tangutorum explants for 48 h significantly increased the rate of AR development from 36% in the untreated control group to 100%. Transcriptome analysis revealed sucrose exerts a significant influence on H2O2 homeostasis and hormone signal transduction pathways. Sucrose treatment promoted IAA biosynthesis through the upregulation of TSA, TSB, and YUC genes and suppressed gibberellin (GA) and cytokinin biosynthesis by downregulating CPS, GA3ox, GA20ox, and LOG. Moreover, sucrose induced abscisic acid (ABA) and salicylic acid (SA) accumulation while reducing JA-Ile, and activated H2O2 signaling through the upregulation of RBOH. Exogenous H2O2 (1-8 mM) mimicked the effects of sucrose; however, this effect was abolished by the auxin transport inhibitor TIBA, indicating that the effects of H2O2 depend on auxin signaling. These results reflect that sucrose acts as an energy substrate as well as a signaling molecule to coordinate hormonal interactions and redox signaling during drought-adaptive AR formation.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.