Sucrose Enhances Adventitious Root Formation in Nitraria tangutorum Under Drought Stress via Hormonal Homeostasis and Hydrogen Peroxide Signaling.

IF 3.6 2区 生物学 Q1 PLANT SCIENCES
Dazhuang Qi, Huirong Xiao, Huihui Liu, Linlin Zheng, Yingchun Wang
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引用次数: 0

Abstract

The robust adventitious root (AR) system of Nitraria tangutorum facilitates its adaptation to drought stress; however, the molecular mechanisms governing AR formation during drought remain unclear. In this study, we demonstrated that a sucrose (40 g L-1) treatment of N. tangutorum explants for 48 h significantly increased the rate of AR development from 36% in the untreated control group to 100%. Transcriptome analysis revealed sucrose exerts a significant influence on H2O2 homeostasis and hormone signal transduction pathways. Sucrose treatment promoted IAA biosynthesis through the upregulation of TSA, TSB, and YUC genes and suppressed gibberellin (GA) and cytokinin biosynthesis by downregulating CPS, GA3ox, GA20ox, and LOG. Moreover, sucrose induced abscisic acid (ABA) and salicylic acid (SA) accumulation while reducing JA-Ile, and activated H2O2 signaling through the upregulation of RBOH. Exogenous H2O2 (1-8 mM) mimicked the effects of sucrose; however, this effect was abolished by the auxin transport inhibitor TIBA, indicating that the effects of H2O2 depend on auxin signaling. These results reflect that sucrose acts as an energy substrate as well as a signaling molecule to coordinate hormonal interactions and redox signaling during drought-adaptive AR formation.

蔗糖通过激素稳态和过氧化氢信号促进干旱胁迫下白刺不定根的形成。
白刺强健的不定根系统有助于其适应干旱胁迫;然而,干旱期间控制AR形成的分子机制仍不清楚。在这项研究中,我们证明了糖(40 g L-1)处理白藜芦醇外植体48小时显著提高了AR的发生率,从未处理对照组的36%提高到100%。转录组分析显示,蔗糖对H2O2稳态和激素信号转导通路有显著影响。蔗糖处理通过上调TSA、TSB和YUC基因促进IAA的生物合成,通过下调CPS、GA3ox、GA20ox和LOG基因抑制赤霉素和细胞分裂素的生物合成。此外,蔗糖在减少JA-Ile的同时诱导脱落酸(ABA)和水杨酸(SA)积累,并通过上调RBOH激活H2O2信号。外源H2O2 (1 ~ 8 mM)模拟蔗糖的作用;然而,这种作用被生长素运输抑制剂TIBA消除,这表明H2O2的作用依赖于生长素信号传导。这些结果反映了蔗糖在干旱适应性AR形成过程中作为能量底物和信号分子协调激素相互作用和氧化还原信号。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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