{"title":"HvNF-YB7: hvgstf13.1介导的大麦谷胱甘肽抗氧化系统的关键调控因子","authors":"Rui Pan, Badr Alharthi, Tiantian Wu, Lin Wang, Jingqiu Cheng, Sajid Fiaz","doi":"10.1111/ppl.70516","DOIUrl":null,"url":null,"abstract":"<p><p>Drought stress severely constrains barley yield and quality improvement. The Nuclear Factor Y (NF-Y) transcription factor family plays a crucial role in plant stress responses; however, its biological function and molecular regulatory mechanism in barley under drought stress remain unclear. This study integrated evolutionary analysis and transcriptomics to reveal the characteristics of the barley NF-Y gene family. Molecular biology experiments were employed to elucidate the drought response function and regulatory mechanism of the core gene HvNF-YB7. Phylogenetic analysis indicated evolutionary conservation and lineage-specific expansion patterns within the NF-Y gene family. The results found that HvNF-YB7 was induced 9.63-fold by drought in the tolerant wild barley genotype EC_S1. An 8-bp deletion variant in HvNF-YB7's promoter region created a CAAT-box cis-acting element, significantly enhancing its transcriptional activity under drought stress. Functional validation using transgenic overexpression significantly improved drought tolerance (e.g., fresh weight, water content), whereas silencing by virus-induced gene silencing (VIGS) exacerbated damage. This confirmed HvNF-YB7 as a key positive regulator. The yeast two-hybrid (Y2H) and RUBY-luciferase assay revealed that HvNF-YB7 forms a transcriptional complex by interacting with NF-YA3 and then recognizes and activates the CCAAT element in the promoter of HvGSTF13.1, a key gene in the glutathione metabolic pathway. This activation enhances glutathione (GSH)-mediated reactive oxygen species (ROS) scavenging capacity, effectively mitigating drought-induced oxidative damage. Our findings elucidate the \"HvNF-YB7-HvGSTF13.1-GSH\" pathway, which enhances barley drought tolerance, and provide a significant theoretical foundation and genetic resources for understanding its molecular basis.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70516"},"PeriodicalIF":3.6000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"HvNF-YB7: A Key Regulator of the HvGSTF13.1-Mediated Glutathione Antioxidant System in Drought-Tolerant Barley.\",\"authors\":\"Rui Pan, Badr Alharthi, Tiantian Wu, Lin Wang, Jingqiu Cheng, Sajid Fiaz\",\"doi\":\"10.1111/ppl.70516\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Drought stress severely constrains barley yield and quality improvement. The Nuclear Factor Y (NF-Y) transcription factor family plays a crucial role in plant stress responses; however, its biological function and molecular regulatory mechanism in barley under drought stress remain unclear. This study integrated evolutionary analysis and transcriptomics to reveal the characteristics of the barley NF-Y gene family. Molecular biology experiments were employed to elucidate the drought response function and regulatory mechanism of the core gene HvNF-YB7. Phylogenetic analysis indicated evolutionary conservation and lineage-specific expansion patterns within the NF-Y gene family. The results found that HvNF-YB7 was induced 9.63-fold by drought in the tolerant wild barley genotype EC_S1. An 8-bp deletion variant in HvNF-YB7's promoter region created a CAAT-box cis-acting element, significantly enhancing its transcriptional activity under drought stress. Functional validation using transgenic overexpression significantly improved drought tolerance (e.g., fresh weight, water content), whereas silencing by virus-induced gene silencing (VIGS) exacerbated damage. This confirmed HvNF-YB7 as a key positive regulator. The yeast two-hybrid (Y2H) and RUBY-luciferase assay revealed that HvNF-YB7 forms a transcriptional complex by interacting with NF-YA3 and then recognizes and activates the CCAAT element in the promoter of HvGSTF13.1, a key gene in the glutathione metabolic pathway. This activation enhances glutathione (GSH)-mediated reactive oxygen species (ROS) scavenging capacity, effectively mitigating drought-induced oxidative damage. Our findings elucidate the \\\"HvNF-YB7-HvGSTF13.1-GSH\\\" pathway, which enhances barley drought tolerance, and provide a significant theoretical foundation and genetic resources for understanding its molecular basis.</p>\",\"PeriodicalId\":20164,\"journal\":{\"name\":\"Physiologia plantarum\",\"volume\":\"177 5\",\"pages\":\"e70516\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physiologia plantarum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1111/ppl.70516\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70516","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
HvNF-YB7: A Key Regulator of the HvGSTF13.1-Mediated Glutathione Antioxidant System in Drought-Tolerant Barley.
Drought stress severely constrains barley yield and quality improvement. The Nuclear Factor Y (NF-Y) transcription factor family plays a crucial role in plant stress responses; however, its biological function and molecular regulatory mechanism in barley under drought stress remain unclear. This study integrated evolutionary analysis and transcriptomics to reveal the characteristics of the barley NF-Y gene family. Molecular biology experiments were employed to elucidate the drought response function and regulatory mechanism of the core gene HvNF-YB7. Phylogenetic analysis indicated evolutionary conservation and lineage-specific expansion patterns within the NF-Y gene family. The results found that HvNF-YB7 was induced 9.63-fold by drought in the tolerant wild barley genotype EC_S1. An 8-bp deletion variant in HvNF-YB7's promoter region created a CAAT-box cis-acting element, significantly enhancing its transcriptional activity under drought stress. Functional validation using transgenic overexpression significantly improved drought tolerance (e.g., fresh weight, water content), whereas silencing by virus-induced gene silencing (VIGS) exacerbated damage. This confirmed HvNF-YB7 as a key positive regulator. The yeast two-hybrid (Y2H) and RUBY-luciferase assay revealed that HvNF-YB7 forms a transcriptional complex by interacting with NF-YA3 and then recognizes and activates the CCAAT element in the promoter of HvGSTF13.1, a key gene in the glutathione metabolic pathway. This activation enhances glutathione (GSH)-mediated reactive oxygen species (ROS) scavenging capacity, effectively mitigating drought-induced oxidative damage. Our findings elucidate the "HvNF-YB7-HvGSTF13.1-GSH" pathway, which enhances barley drought tolerance, and provide a significant theoretical foundation and genetic resources for understanding its molecular basis.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.