{"title":"酒花种质耐盐碱性评价及其对盐碱胁迫的形态生理响应。","authors":"Siyao Chen, Yanjie Li, Wenlong Zhang, Huizhen Shen, Siyu Wang, Rui Wang, Haiyan Li","doi":"10.1111/ppl.70541","DOIUrl":null,"url":null,"abstract":"<p><p>Soil salinization is a severe abiotic stress that affects plant growth and development, leading to accelerated maturity and decreased production and quality. In the present study, we evaluated 170 Oenothera L. germplasms for saline-alkali tolerance by subjecting them to 150 mmol·L<sup>-1</sup> saline-alkali stress (pH = 9.0). Germination parameters were analyzed, and comprehensive evaluation methods were used to assess their tolerance at the germination stage. Among the 170 germplasms, we identified 12 saline-alkali-tolerant and 34 saline-alkali-sensitive germplasms. Subsequently, 23 germplasms were further evaluated for saline-alkali tolerance at the seedling stage. While seedling growth was inhibited across all specimens, five saline-alkali-tolerant germplasms were ultimately selected through comprehensive evaluation methods. We further examined photosynthetic pigment levels, membrane permeability, antioxidant oxidases, osmotic regulators, and mineral content to determine the plants' response. Germplasms with a higher tolerance to saline-alkali stress can adapt by (i) maintaining or increasing chlorophyll content and delaying leaf senescence, (ii) using osmotic adjustment by maintaining stable osmotic potential, (iii) maintaining relatively stable electrical conductivity, (iv) enhancing antioxidant systems and maintaining a relatively low level of malondialdehyde, and (v) utilizing ion adjustment by promoting higher accumulation of Ca<sup>2+</sup>, Mg<sup>2+</sup>, and Mn<sup>2+</sup> in the roots and ensuring higher ratios of K<sup>+</sup>/Na<sup>+</sup>, Ca<sup>2+</sup>/Na<sup>+</sup>, and Mg<sup>2+</sup>/Na<sup>+</sup> in the leaves. These findings could serve as a valuable reference for future investigations of genetic mechanisms underlying saline-alkali tolerance in Oenothera L. species and provide a foundation for the conservation of germplasm resources, genetic improvement, and application of Oenothera L. species in saline-alkali environments.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 5","pages":"e70541"},"PeriodicalIF":3.6000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of Saline-Alkali Tolerance of Oenothera L. Germplasms and Their Morpho-Physiological Responses to Saline-Alkali Stress.\",\"authors\":\"Siyao Chen, Yanjie Li, Wenlong Zhang, Huizhen Shen, Siyu Wang, Rui Wang, Haiyan Li\",\"doi\":\"10.1111/ppl.70541\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Soil salinization is a severe abiotic stress that affects plant growth and development, leading to accelerated maturity and decreased production and quality. In the present study, we evaluated 170 Oenothera L. germplasms for saline-alkali tolerance by subjecting them to 150 mmol·L<sup>-1</sup> saline-alkali stress (pH = 9.0). Germination parameters were analyzed, and comprehensive evaluation methods were used to assess their tolerance at the germination stage. Among the 170 germplasms, we identified 12 saline-alkali-tolerant and 34 saline-alkali-sensitive germplasms. Subsequently, 23 germplasms were further evaluated for saline-alkali tolerance at the seedling stage. While seedling growth was inhibited across all specimens, five saline-alkali-tolerant germplasms were ultimately selected through comprehensive evaluation methods. We further examined photosynthetic pigment levels, membrane permeability, antioxidant oxidases, osmotic regulators, and mineral content to determine the plants' response. Germplasms with a higher tolerance to saline-alkali stress can adapt by (i) maintaining or increasing chlorophyll content and delaying leaf senescence, (ii) using osmotic adjustment by maintaining stable osmotic potential, (iii) maintaining relatively stable electrical conductivity, (iv) enhancing antioxidant systems and maintaining a relatively low level of malondialdehyde, and (v) utilizing ion adjustment by promoting higher accumulation of Ca<sup>2+</sup>, Mg<sup>2+</sup>, and Mn<sup>2+</sup> in the roots and ensuring higher ratios of K<sup>+</sup>/Na<sup>+</sup>, Ca<sup>2+</sup>/Na<sup>+</sup>, and Mg<sup>2+</sup>/Na<sup>+</sup> in the leaves. These findings could serve as a valuable reference for future investigations of genetic mechanisms underlying saline-alkali tolerance in Oenothera L. species and provide a foundation for the conservation of germplasm resources, genetic improvement, and application of Oenothera L. species in saline-alkali environments.</p>\",\"PeriodicalId\":20164,\"journal\":{\"name\":\"Physiologia plantarum\",\"volume\":\"177 5\",\"pages\":\"e70541\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physiologia plantarum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1111/ppl.70541\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70541","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Evaluation of Saline-Alkali Tolerance of Oenothera L. Germplasms and Their Morpho-Physiological Responses to Saline-Alkali Stress.
Soil salinization is a severe abiotic stress that affects plant growth and development, leading to accelerated maturity and decreased production and quality. In the present study, we evaluated 170 Oenothera L. germplasms for saline-alkali tolerance by subjecting them to 150 mmol·L-1 saline-alkali stress (pH = 9.0). Germination parameters were analyzed, and comprehensive evaluation methods were used to assess their tolerance at the germination stage. Among the 170 germplasms, we identified 12 saline-alkali-tolerant and 34 saline-alkali-sensitive germplasms. Subsequently, 23 germplasms were further evaluated for saline-alkali tolerance at the seedling stage. While seedling growth was inhibited across all specimens, five saline-alkali-tolerant germplasms were ultimately selected through comprehensive evaluation methods. We further examined photosynthetic pigment levels, membrane permeability, antioxidant oxidases, osmotic regulators, and mineral content to determine the plants' response. Germplasms with a higher tolerance to saline-alkali stress can adapt by (i) maintaining or increasing chlorophyll content and delaying leaf senescence, (ii) using osmotic adjustment by maintaining stable osmotic potential, (iii) maintaining relatively stable electrical conductivity, (iv) enhancing antioxidant systems and maintaining a relatively low level of malondialdehyde, and (v) utilizing ion adjustment by promoting higher accumulation of Ca2+, Mg2+, and Mn2+ in the roots and ensuring higher ratios of K+/Na+, Ca2+/Na+, and Mg2+/Na+ in the leaves. These findings could serve as a valuable reference for future investigations of genetic mechanisms underlying saline-alkali tolerance in Oenothera L. species and provide a foundation for the conservation of germplasm resources, genetic improvement, and application of Oenothera L. species in saline-alkali environments.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.