Yinhong Xiang, Hao Yang, Yani Zhong, Xunuo Ren, Jing Wen, Zhong Wang, Xueshuang Huang, Jianxin Liu, Yong Huang, Hong Pu
{"title":"Ellagic acid C and aspergone R, two undescribed pyrones from the endophytic fungus <i>Diaporthe</i> sp. CB10100: biological evaluation, molecular docking and molecular dynamics studies.","authors":"Yinhong Xiang, Hao Yang, Yani Zhong, Xunuo Ren, Jing Wen, Zhong Wang, Xueshuang Huang, Jianxin Liu, Yong Huang, Hong Pu","doi":"10.1080/14786419.2025.2565814","DOIUrl":"https://doi.org/10.1080/14786419.2025.2565814","url":null,"abstract":"<p><p>Two new compounds, ellagic acid C (<b>5</b>) and aspergone R (<b>6</b>), along with four previously identified compounds (<b>7-10</b>), were successfully isolated from the fungus <i>Diaporthe</i> sp. CB10100. Ellagic acid C (<b>5</b>) contains an uncommon tricyclic 5/6/6 ring system. A biosynthetic pathway for <b>6</b> was proposed, although additional investigations are needed to elucidate the ethyl biosynthesis mechanism. Both aspergone R (<b>6</b>) and (-)-terpestacin (<b>9</b>) decreased lipopolysaccharide (LPS)-induced protein expression of iNOS and COX-2. Molecular docking analysis revealed that <b>6</b> and <b>9</b> potentially interact with the active sites of iNOS and COX-2. Molecular dynamics simulations demonstrated that, compared with the other compounds, <b>6</b> exhibited increased binding stability and stronger interactions with the COX-2 protein. Furthermore, <b>5-9</b> were effective against MRSA, <i>Klebsiella pneumoniae</i>, and <i>Mycolicibacterium</i> (<i>Mycobacterium</i>) smegmatis, with MICs greater than 64 μg/mL. These findings suggest that <b>6</b> and the common γ-pyrone scaffold could serve lead compounds for anti-inflammatory drug development.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hao Zheng, Kaiwen Kang, Yujia Zhang, Ligen Lin, Lishe Gan
{"title":"Lactucin enhances glucose uptake and glycolysis in C2C12 myotubes by activating AMPK signaling pathway.","authors":"Hao Zheng, Kaiwen Kang, Yujia Zhang, Ligen Lin, Lishe Gan","doi":"10.1080/14786419.2025.2567637","DOIUrl":"https://doi.org/10.1080/14786419.2025.2567637","url":null,"abstract":"<p><p>Lactucin is a sesquiterpenoid from the well-known traditional Chinese medicine <i>Cichorium intybus</i> L. The antidiabetic effect of lactucin and its underlying mechanism remain unclear. Using fluorescence-labelled glucose analogue, we found that lactucin dose-dependently increased glucose uptake through upregulating expression and membrane translocation of GLUT4 in C2C12 myotubes. These effects were mediated by AMP-activated protein kinase (AMPK) activation, which was abolished by the co-treatment of Compound C or <i>Ampkα1</i> silencing. Furthermore, lactucin induced a controlled suppression of mitochondrial function, as evidenced by decreased mitochondrial membrane potential and ATP levels, thereby mimicking cellular energy stress to activate AMPK. The metabolic reprogramming subsequently enhanced glycolysis and reduced intracellular lipid accumulation in C2C12 myotubes. Taken together, lactucin induces a controlled mitochondrial suppression and mimics exercise-like energy stress, which in turn enhances glucose uptake in C2C12 myotubes. We firstly uncover the metabolic reprogramming effect of lactucin, suggesting its potential as an antidiabetic agent.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-7"},"PeriodicalIF":1.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xian-Hua He, Ye-Fang Hu, Hua-Chun Feng, Li-Qi Liang, Lan-Fang Zou, Qian Wu, Min Liang, Jun Li, Wei-Feng Xu, Rui-Yun Yang
{"title":"Antifungal cytochalasans from the co-culture of two endophytic fungi <i>Aspergillus spelaeus</i> and <i>Xylaria</i> sp.","authors":"Xian-Hua He, Ye-Fang Hu, Hua-Chun Feng, Li-Qi Liang, Lan-Fang Zou, Qian Wu, Min Liang, Jun Li, Wei-Feng Xu, Rui-Yun Yang","doi":"10.1080/14786419.2025.2566463","DOIUrl":"https://doi.org/10.1080/14786419.2025.2566463","url":null,"abstract":"<p><p>A new cytochalasan, sophochalasin (<b>1</b>), together with ten known analogous compounds were obtained from the co-culture of two endophytic fungi <i>Aspergillus spelaeus</i> and <i>Xylaria</i> sp. The structure and the absolute configuration of <b>1</b> were established <i>via</i> NMR and ECD spectral analysis. In antifungal assay, aspochalasin B (<b>2</b>) exhibited notable antifungal activity against <i>Fusarium oxysporum</i>, <i>Colletotrichum siamense</i>, <i>Alternaria alternata</i> and <i>Fusarium oxysporium</i> f. sp. <i>cucumberinum</i> with MICs of 16 <i>μ</i>g/mL.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dubiusine isolated from <i>Adenoon indicum dalz</i> plant induces apoptosis in human colon cancer cells.","authors":"Ajit Patil, Amruta Joshi","doi":"10.1080/14786419.2025.2565813","DOIUrl":"https://doi.org/10.1080/14786419.2025.2565813","url":null,"abstract":"<p><p>Dubiusine, an unusual alkaloid, extracted from the leaves of the medicinal plant, <i>Adenoon indicum Dalz</i> for the first time and evaluated it is <i>in vitro</i> and <i>in silico</i> cytotoxic potential in colorectal cancer (CRC). The anti-proliferative effect of the dubiusine was found to be dose-dependent with IC<sub>50</sub> values of 27.95 µM, 30 µM, 30.59 µM on HTC-15, HTC-16, HT-29 cell lines respectively. Dubiusine inhibited CRC cell proliferation, induced late apoptosis, and arrested the cell cycle at the G2/M phase. Mechanistically, dubiusine down regulated the expression of anti-apoptotic proteins Bcl-2, Bcl-x and up regulated the expression of initiator and executioner caspases, that leads to apoptosis. The in silico study showed potential binding affinity of dubiusine at different caspase proteins and dynamic simulation study confirmed the structural integrity and stability of caspase-dubiusine complex.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-7"},"PeriodicalIF":1.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yan Liu, Jin-Hong Xue, Yan Sun, Fan-Ying Deng, Si-Yi Wang, Juan Pan, Zhi-Chao Hao, Ke-Zhen Chen, Anam Naseem, Qing-Shan Chen, Li-Li Zhang, Hai-Xue Kuang, Bing-You Yang
{"title":"Three new aliphatic compounds from the roots of <i>Saposhnikovia divaricata</i> and their antiproliferative activity on HepG2 cells.","authors":"Yan Liu, Jin-Hong Xue, Yan Sun, Fan-Ying Deng, Si-Yi Wang, Juan Pan, Zhi-Chao Hao, Ke-Zhen Chen, Anam Naseem, Qing-Shan Chen, Li-Li Zhang, Hai-Xue Kuang, Bing-You Yang","doi":"10.1080/14786419.2025.2566468","DOIUrl":"https://doi.org/10.1080/14786419.2025.2566468","url":null,"abstract":"<p><p>The phytochemical investigation of <i>Saposhnikovia divaricata</i> (Turcz.) Schischk's roots resulted in the isolation of 16 distinct compounds, including two new aliphatics: sadiesteroside A (1) and 2,3,4-trihydroxyundecanoic acid (2), one new aliphatic hydrocarbon methyl propionate-3-(2-hydroxy-propionate) (3), and thirteen known aliphatics (4-16). The structural elucidation of compounds 1-16 was established by 1D and 2D NMR spectroscopy, supported by high-resolution mass spectrometric analysis and literature references. All isolated compounds' <i>in vitro</i> antiproliferative potential was evaluated using HepG2 cells. The results indicated that compound 3, compound 15, and compound 16 significantly inhibited the activity of HepG2 cells.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Majid Khalil Abad Halimi, Mohabat Nadaf, Ali Riahi-Madvar, Mahdieh Rostami
{"title":"Investigating seasonal variations in composition of the <i>Rosmarinus officinalis</i> essential oil in North Khorasan Province (Bojnord), Iran: a comparative analysis with existing literature, focusing on monoterpenol components.","authors":"Majid Khalil Abad Halimi, Mohabat Nadaf, Ali Riahi-Madvar, Mahdieh Rostami","doi":"10.1080/14786419.2025.2565271","DOIUrl":"10.1080/14786419.2025.2565271","url":null,"abstract":"<p><p><i>Rosmarinus officinalis</i> L. (Lamiaceae), a Mediterranean aromatic plant, is valued in cosmetics, cuisine, and pharmaceuticals. This study investigates seasonal variations in essential oil (EO) composition and yield from <i>R. officinalis</i> harvested at Kosar University during 2023-2024. GC-MS and GC analyses revealed EO yields of 0.39-0.77%, with major compounds including α-pinene, camphene, limonene, camphor, and linalool. Seasonal shifts were observed: summer exhibited higher linalool and camphor, autumn peaked in verbenone and 3-octanone, and winter increased monoterpene alcohols (25.28%). Principal Component Analysis (PCA) distinguished three ecological groups (Group1: <b><i>R<sub>3</sub></i></b>; Group2: <b><i>R<sub>4</sub></i></b>, <b><i>R<sub>6</sub></i></b>, <b><i>R<sub>1</sub></i></b>, <b><i>R<sub>2</sub></i></b>; Group3: <b><i>R<sub>5</sub></i></b>), though intra-seasonal chemical differences within regions were minimal. These findings highlight the influence of seasonal dynamics on rosemary's phytochemical profile, offering insights for optimised harvest timing to enhance EO quality for industrial applications.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Reineckeacarnisides A and B, two new saponins from <i>Reineckea carnea</i>.","authors":"Ping Wang, Feiyu Liao, Hongqian Liu, Ruirui Zhang","doi":"10.1080/14786419.2025.2565711","DOIUrl":"https://doi.org/10.1080/14786419.2025.2565711","url":null,"abstract":"<p><p>Reineckeacarnisides A (<b>1</b>) and B (<b>2</b>) were obtained from the roots of <i>Reineckea carnea</i> (Andrews) Kunth. Their structures were deduced by physicochemical properties and spectroscopic methods. Cytotoxic activities in three cancer cell lines were evaluated by CCK8 method and Reineckeacarniside B possessed inhibitory effect with IC<sub>50</sub> of 45.6 μM.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-5"},"PeriodicalIF":1.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Limonoids and other constituents of the stem of <i>Naringi</i> crenulata (Roxb.) Nicholson.","authors":"Suriphon Singha, Sudarat Kruakaew, Jantana Yahuafai, Sanit Thongnest, Somyote Sutthivaiyakit","doi":"10.1080/14786419.2025.2566473","DOIUrl":"https://doi.org/10.1080/14786419.2025.2566473","url":null,"abstract":"<p><p>Five previously undescribed compounds including four limonoids and a phenolic glycoside as well as seven known compounds were isolated from <i>Naringi crenulata</i> (Roxb.) Nicolson stem. Spectroscopic data of these previously undescribed compounds are reported. The configurations at stereocenters in the isolates were based on the NOE interactions. The bio-transformations of three unusual limonoids from limonin were proposed. Selected isolates were evaluated for their growth inhibitory activities against two cancer cell lines and a Vero cell.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation of a novel bufadienolide, bufovende A, and seven known analogs from <i>Venenum bufonis</i> with inhibitory activity on hepatic stellate cell activation.","authors":"Huijing Lv, Chengjin Li, Qinggui Li, Qingqing Luo, Yunsong Chen, Anli Liu, Xinyi Du, Qian Cao, Pengwei Zhang, Lingjie Meng","doi":"10.1080/14786419.2025.2567641","DOIUrl":"https://doi.org/10.1080/14786419.2025.2567641","url":null,"abstract":"<p><p>As the principal bioactive constituents of <i>Venenum Bufonis</i> (VB), bufadienolides mediate the therapeutic efficacy of this traditional Chinese medicine in diverse clinical applications. In this study, a novel bufadienolide, designated as bufovende A (<b>1</b>), and seven known analogs (<b>2-8</b>) were isolated from VB. The structure of bufovende A was determined using spectroscopic methods (1D/2D NMR and HRESIMS). Given the pivotal role of hepatic stellate cell (HSC) activation in liver fibrogenesis, we evaluated the antifibrotic potential of these compounds using the LX-2 human HSC line. The results indicated that non-cytotoxic concentrations (0.2 μM) of bufadienolides significantly suppressed the mRNA expression of fibrosis biomarkers α-SMA and COL1A1, with bufovende A exhibiting the most pronounced inhibitory efficacy (inhibition rates of 61.33% ± 1.54% for α-SMA and 57.46% ± 12.03% for COL1A1). Additionally, compared to the model group, bufovende A downregulated α-SMA, COL1A1, and TGF-β protein expression. These results highlight the potential of bufadienolides as promising antifibrotic leads, with bufovende A being particularly promising for further investigation.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Resveratrol synergistically enhances anti-lung cancer efficacy with myricetin and dihydromyricetin on A549 cells by increasing their intracellular concentrations.","authors":"Yuexing Chang, Yufeng Liu, Ailing Guo, Liangfeng Wang, Zhihuan Wu, Haodong Jiang, Fangli Wang, Yinyin Xiang, Feng Jin, Yun Deng","doi":"10.1080/14786419.2025.2566912","DOIUrl":"https://doi.org/10.1080/14786419.2025.2566912","url":null,"abstract":"<p><p>The present study aimed to investigate the synergistic cytotoxic effects and related mechanism of resveratrol and myricetin or dihydromyricetin in A549 cells. Cell viability, scratch wound, clone formation, cell apoptosis and cycle test were operated to confirm the synergistic anti-lung cancer effects. The effects of resveratrol on the efflux of myricetin, dihydromyricetin and rhodamine 123 were determined to discover the synergistic mechanism. The combination of resveratrol of 25 µg/mL and myricetin or dihydromyricetin of 25 µg/mL had the best synergistic anti-lung cancer effect. Resveratrol combined with myricetin or dihydromyricetin caused A549 cells to exit the S- and G2/M-phase. Resveratrol enhanced the myricetin, dihydromyricetin and rhodamine 123 concentrations in A549 cells. Resveratrol and myricetin or dihydromyricetin exert a synergistic effect, but not <i>via</i> a suppression of P-glycoprotein.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}