{"title":"从植物中分离的双歧杆菌诱导人结肠癌细胞凋亡。","authors":"Ajit Patil, Amruta Joshi","doi":"10.1080/14786419.2025.2565813","DOIUrl":null,"url":null,"abstract":"<p><p>Dubiusine, an unusual alkaloid, extracted from the leaves of the medicinal plant, <i>Adenoon indicum Dalz</i> for the first time and evaluated it is <i>in vitro</i> and <i>in silico</i> cytotoxic potential in colorectal cancer (CRC). The anti-proliferative effect of the dubiusine was found to be dose-dependent with IC<sub>50</sub> values of 27.95 µM, 30 µM, 30.59 µM on HTC-15, HTC-16, HT-29 cell lines respectively. Dubiusine inhibited CRC cell proliferation, induced late apoptosis, and arrested the cell cycle at the G2/M phase. Mechanistically, dubiusine down regulated the expression of anti-apoptotic proteins Bcl-2, Bcl-x and up regulated the expression of initiator and executioner caspases, that leads to apoptosis. The in silico study showed potential binding affinity of dubiusine at different caspase proteins and dynamic simulation study confirmed the structural integrity and stability of caspase-dubiusine complex.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-7"},"PeriodicalIF":1.6000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dubiusine isolated from <i>Adenoon indicum dalz</i> plant induces apoptosis in human colon cancer cells.\",\"authors\":\"Ajit Patil, Amruta Joshi\",\"doi\":\"10.1080/14786419.2025.2565813\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Dubiusine, an unusual alkaloid, extracted from the leaves of the medicinal plant, <i>Adenoon indicum Dalz</i> for the first time and evaluated it is <i>in vitro</i> and <i>in silico</i> cytotoxic potential in colorectal cancer (CRC). The anti-proliferative effect of the dubiusine was found to be dose-dependent with IC<sub>50</sub> values of 27.95 µM, 30 µM, 30.59 µM on HTC-15, HTC-16, HT-29 cell lines respectively. Dubiusine inhibited CRC cell proliferation, induced late apoptosis, and arrested the cell cycle at the G2/M phase. Mechanistically, dubiusine down regulated the expression of anti-apoptotic proteins Bcl-2, Bcl-x and up regulated the expression of initiator and executioner caspases, that leads to apoptosis. The in silico study showed potential binding affinity of dubiusine at different caspase proteins and dynamic simulation study confirmed the structural integrity and stability of caspase-dubiusine complex.</p>\",\"PeriodicalId\":18990,\"journal\":{\"name\":\"Natural Product Research\",\"volume\":\" \",\"pages\":\"1-7\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Natural Product Research\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1080/14786419.2025.2565813\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Product Research","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1080/14786419.2025.2565813","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Dubiusine isolated from Adenoon indicum dalz plant induces apoptosis in human colon cancer cells.
Dubiusine, an unusual alkaloid, extracted from the leaves of the medicinal plant, Adenoon indicum Dalz for the first time and evaluated it is in vitro and in silico cytotoxic potential in colorectal cancer (CRC). The anti-proliferative effect of the dubiusine was found to be dose-dependent with IC50 values of 27.95 µM, 30 µM, 30.59 µM on HTC-15, HTC-16, HT-29 cell lines respectively. Dubiusine inhibited CRC cell proliferation, induced late apoptosis, and arrested the cell cycle at the G2/M phase. Mechanistically, dubiusine down regulated the expression of anti-apoptotic proteins Bcl-2, Bcl-x and up regulated the expression of initiator and executioner caspases, that leads to apoptosis. The in silico study showed potential binding affinity of dubiusine at different caspase proteins and dynamic simulation study confirmed the structural integrity and stability of caspase-dubiusine complex.
期刊介绍：
The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds.
The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal.
Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section.
All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.
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