Moise Diguir, Bernard Dabole, Isaac Silvère Gade, Peron Bosco Leutcha, Honore Wangso, Matthieu Matcheme, Albert Atangana Fouda, Jean Noël Nyemb, Moussa Djaouda, Alessandro Venditti, Alex De Theodore Atchadé, Benoit Koubala Bargui
{"title":"A new limonoid, digsecokigelianolide, among the secondary metabolites from <i>kigelia africana</i> (lam.) benth. and evaluation of their antibacterial and antioxidant activity.","authors":"Moise Diguir, Bernard Dabole, Isaac Silvère Gade, Peron Bosco Leutcha, Honore Wangso, Matthieu Matcheme, Albert Atangana Fouda, Jean Noël Nyemb, Moussa Djaouda, Alessandro Venditti, Alex De Theodore Atchadé, Benoit Koubala Bargui","doi":"10.1080/14786419.2025.2568952","DOIUrl":"https://doi.org/10.1080/14786419.2025.2568952","url":null,"abstract":"<p><p>The present work investigates the phytochemical study and the antibacterial and antioxidant activities of <i>Kigelia africana</i> (Lam.) Benth. Column chromatography separation on silica gel of fractions led to the isolation of a new limonoid derivative named digsecokigelianolide (<b>1</b>), as well as nine known compounds (<b>2-10</b>). The structures of the isolated compounds were determined by comprehensive spectroscopic analysis, including 1 & 2D NMR, IR, UV, mass spectrometry and comparison with previous reported data. Compound <b>1</b> showed moderate antiradical activity (IC<sub>50</sub> = 181.12 µg/mL) against ABST<sup>•+</sup> radical compared to BHT (159.48 µg/mL) used as standard, and a good antibacterial activity against <i>Salmonella typhi</i> (MIC: 22.0 mm), <i>Escherichia coli</i> (MIC: 15.0 mm) and <i>Pseudomonas aeruginosa</i> (MIC: 20 mm) at the concentration of 25 mg/mL. Compound 9 and 10 showed good antiradical (which ranges from 56.39 to 61.16% of inhibition) and antibacterial (which ranges from 12 to 20 mm of inhibition zone) activities.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-10"},"PeriodicalIF":1.6,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ingrid Reis da Silva, Yasmin Cunha da Silva, Mauricio Morishi Ogusku, Valdir Florêncio da Veiga-Junior, Rudi Emerson de Lima Procópio
{"title":"Antimicrobial actinomycins isolated by bioautography from Amazonian soil <i>Streptomyces collinus</i> Tü broth.","authors":"Ingrid Reis da Silva, Yasmin Cunha da Silva, Mauricio Morishi Ogusku, Valdir Florêncio da Veiga-Junior, Rudi Emerson de Lima Procópio","doi":"10.1080/14786419.2025.2566906","DOIUrl":"https://doi.org/10.1080/14786419.2025.2566906","url":null,"abstract":"<p><p>The aim of this study was to isolate and identify bioactive compounds through a bioactivity-guided analysis of extracts from <i>Streptomyces</i> strains. A bioactive extract from a <i>Streptomyces</i> species isolated from Amazonian soil, identified as <i>S. collinus</i> Tü 365 through the 16S rRNA and 23S rRNA genes, was selected for the study. The fermentation broth extract was partitioned and the bioactive fractions were further fractionated using chromatographic techniques (preparative TLC and HPLC). Through bioautography-guided fractionation, two actinomycins (D and X2) were isolated and identified by spectrometric (ESI-HRMSMS) and spectroscopic (NMR) techniques. The isolated substances were tested in the agar disc diffusion assay (<i>Staphylococcus aureus</i>, <i>Streptococcus pneumoniae</i>, <i>Enterococcus faecalis</i> and <i>Escherichia coli</i>) and by the microdilution method in microplates (<i>Staphylococcus aureus</i> and <i>Mycobacterium smegmatis</i>). Actinomycins D and X2 showed activity against <i>M. smegmatis</i> 16.0 μg/mL for both samples and against <i>S. aureus</i>, 1 μg/mL and 0.5 μg/mL, respectively.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.6,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zahra Fodil, Abdelkader Benhelima, Ali Boumeftah, Jean-Marie Lacroix, Anissa Ameur, Ines Youcef Achira, Salah Merah, Noureddine Ghali
{"title":"Chemical constituents and antimicrobial activity of the waste of date palm (<i>Phoenix dactylifera</i> L., arecaceae).","authors":"Zahra Fodil, Abdelkader Benhelima, Ali Boumeftah, Jean-Marie Lacroix, Anissa Ameur, Ines Youcef Achira, Salah Merah, Noureddine Ghali","doi":"10.1080/14786419.2025.2570505","DOIUrl":"https://doi.org/10.1080/14786419.2025.2570505","url":null,"abstract":"<p><p>The chemical composition and antimicrobial activity against bacterial and fungal strains related to various diseases of different parts (leaf, fibre and fruit bunch) from waste of date palm (<i>Phoenix dactylifera</i> L.) grown in southern Algeria were studied. HPLC-DAD showed that Quercetin was the most dominant polyphenolic component in all extracts. The antimicrobial activity test shows that leaf waste extract has the highest activity than fibre and fruit bunch waste extracts with inhibitory zone diameter (IZD) of 17.8, 12.1 and 14 mm against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and <i>Candida albicans</i>, respectively. This agro-waste matrix may represent an important source not only of phenolic acid compounds but also of other bioactive molecules important for health. The valorisation of biowaste from date palm offers strategies to reduce waste and exploit potentially harmful health benefits and may be a valuable source of antimicrobial agents.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.6,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"One new ten-membered macrolide and one new pyridinone derivative from the mangrove endophytic fungus <i>Alternaria porri #</i>25.","authors":"Fengting Tang, Namei Zheng, Yuhui Wang, Jinjun Li, Ronglan Tan, Zhiyun Du, Huarong Huang, Guangying Chen","doi":"10.1080/14786419.2025.2570894","DOIUrl":"https://doi.org/10.1080/14786419.2025.2570894","url":null,"abstract":"<p><p>One new ten-membered macrolide derivative, porriolide (<b>1</b>), and one new 2(1H)-pyridinone derivative (±)-porripyridone (<b>2</b>), together with seven known compounds alternariol 4-methyl ether (<b>3</b>), alternariol (<b>4</b>), 5'-methoxy-6-methylbiphenyl-3,4,3'-triol (<b>5</b>), altenusinoide A (<b>6</b>), harziano pyridone (<b>7</b>), penicilloxalone A (<b>8</b>), and cytosporin D (<b>9</b>) were obtained from mangrove endophytic fungus <i>Alternaria porri</i> #25. Their structures were elucidated using comprehensive spectroscopic methods, and the absolute configuration of the new compounds were further confirmed by ECD spectra and single-crystal X-ray diffraction analysis, respectively. Compounds <b>2</b> and <b>7</b> exhibited cytotoxic activities against prostate cancer VCap and PC-3 cells with IC<sub>50</sub> values ranging from 22.94 ± 0.53 μmol/L to 38.13 ± 0.93 μmol/L, while compound <b>1</b> showed no cytotoxic activity.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.6,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yongbai Liang, Xican Li, Xi Zhao, Rongxin Cai, Shaoman Chen, Rongrong Lin, Hanxiao Chai, Chunhou Li
{"title":"Simultaneous semi-quantification and docking simulation of 43 compounds reveal bioactive difference between phenolics and alkaloids in fresh edible nut of <i>Areca catechu</i> L.","authors":"Yongbai Liang, Xican Li, Xi Zhao, Rongxin Cai, Shaoman Chen, Rongrong Lin, Hanxiao Chai, Chunhou Li","doi":"10.1080/14786419.2025.2571852","DOIUrl":"https://doi.org/10.1080/14786419.2025.2571852","url":null,"abstract":"<p><p>There is a great controversy about the definition of fresh nut of <i>Areca catechu</i> L. around the world. The study created a database-affinity ultra-high-performance liquid chromatography-quadrupole-orbitrap-tandem mass spectrometry strategy. Through the strategy, a total of 43 compounds were putatively identified in fresh edible <i>Areca catechu</i> nut. Particularly, 17 'unexpected' compounds were found for the first time. Thereafter, their chemical contents were simultaneously measured using the above strategy. Total content of 26 phenolics is nearly one-third of that of 5 alkaloids. However, in the molecular docking simulation towards superoxide dismutase (SOD), phenolics are superior to alkaloids, because they use active H-atom to facilitate H-bonding as well as coupled proton and electron (<b><i>e</i></b>) transfer (CPET) mechanism to exert the antioxidant action. Top 5 phenolics dominate the antioxidant contribution in whole fresh <i>Areca catechu</i> nut, <i>i.e.</i> (+) catechin, protocatechuic acid, 2″-<i>O</i>-β-L-galactopyranosylorientin, coniferaldehyde, and syringic acid. Our findings provide basic evidence for its edibility.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.6,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influence of environmental conditions on yield and quality of different cultivars of Ashwagandha (<i>Withania somnifera</i> L. Dunal).","authors":"Anjali Singh, Saudan Singh, Anil Kumar Singh, Prattek Kumar Shasany, Rajesh Kumar Verma","doi":"10.1080/14786419.2025.2566465","DOIUrl":"https://doi.org/10.1080/14786419.2025.2566465","url":null,"abstract":"<p><p>A two-year field experiment (2021-2023) was conducted to assess the yield and quality of Ashwagandha (<i>Withania somnifera</i>) under various environmental conditions. The C1cultivar (NMITLI-118) exhibited the highest yields of dry leaves (0.63 t ha<sup>-1</sup>) and roots (1.08 t ha<sup>-1</sup>) in the summer season (S2). Cultivar NMITLI-118 (C1) performs better in the summer season (S2, February sown), with average temperatures between 30-35 °C, where reduced moisture availability and heat stress stimulate higher synthesis of secondary metabolites and their constituents such as withanolides (0.36%), starch (24.24%), and starch-fibre ratio (0.80). Additionally, higher net returns of 2044.58 USD ha<sup>-1</sup> were obtained with NMITLI-118 during the summer season (S2). Furthermore, this approach allows farmers to utilise the lean period between the Rabi and Kharif seasons effectively treating ashwagandha as a bonus crop. The study also indicates that winter crops might be better for withaferin production, despite lower yields of roots and leaves.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.6,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pharmaceutical potential of traditionally fermented <i>Bambusa balcooa</i> shoots from Assam: a GC-MS-based profiling of bioactive compounds.","authors":"Preety Priyam Saikia, Anil Bora","doi":"10.1080/14786419.2025.2570507","DOIUrl":"https://doi.org/10.1080/14786419.2025.2570507","url":null,"abstract":"<p><p>This study explored the bioactive compounds and pharmaceutical potential of traditionally fermented <i>Bambusa balcooa</i> shoots from Assam, India, using gas chromatography-mass spectrometry (GC-MS). Young shoots were harvested and fermented for 10-15 days following indigenous practices. GC-MS analysis identified 28 volatile bioactive compounds, including ketones (47.35%), esters (26.08%), and sesquiterpenes (10.04%), with 2-undecanone (36.85%) and dodecanoic acid, ethenyl ester (12.41%) as dominant constituents. Nine compounds were evaluated for their pharmaceutical activities. Notably, 2-undecanone and nerolidol exhibited strong antimicrobial and anticancer effects, while n-hexadecanoic acid showed potent antitumour and immune-boosting properties. Compared with other fermented bamboo shoots from Northeast India, the higher levels of nerolidol and undecanone in <i>B. balcooa</i> indicate a stronger potential for anticancer and antiparasitic effects, based on literature evidence. This research underscores the therapeutic potential of fermented <i>Bambusa balcooa</i> shoots as functional foods rooted in Assam's ethnic cuisine, advocating their incorporation into pharmaceutical and nutraceutical applications.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Synthesis and biological studies of novel fusidic acid-linked crown ethers.","authors":"Shumaila Jawaid, Nuzhat Arshad, Syed Nawazish Ali, Jamshed Hashim, Mehreen Lateef, Ayesha Anwar","doi":"10.1080/14786419.2025.2569800","DOIUrl":"https://doi.org/10.1080/14786419.2025.2569800","url":null,"abstract":"<p><p>We successfully prepared novel Fusidic acid-linked crown ethers with various cavity sizes and fully characterised them using <sup>1</sup>H NMR,<sup>13</sup>C NMR (Broadband, DEPT-135, and DEPT-90), and ESI-MS. All the crown ethers and the precursor (Fusidic acid) were examined for their antioxidant and α-glucosidase inhibition activities. The crown ethers demonstrated excellent antioxidant activity, with IC<sub>50</sub> values ranging from 11.4 ± 0.54 to 45.6 ± 0.11 μM. They showed moderate α-glucosidase inhibitory effects, with IC<sub>50</sub> values ranging from 34.2 ± 0.23 to 65.7 ± 0.09 μM, compared to standards Butylated hydroxyanisole (IC<sub>50</sub> = 44.2 ± 0.36 μM) and deoxynojirimycin (IC<sub>50</sub> = 3.9 ± 0.71 μM), respectively. Notably, antioxidant activities increased in the crown ethers compared to parent Fusidic acid, highlighting the role of the crown moiety in enhancing activity. Molecular docking studies on human antioxidant proteins revealed that the most active antioxidant compounds exhibit fewer hydrogen bonds and higher alkyl bond interactions.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-7"},"PeriodicalIF":1.6,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Julia Mirian Paulino, Natália Dos Santos Gonçalves, Heber Eduardo Andrada, Arthur Barcelos Ribeiro, Carlos Henrique Inácio Ramos, Cassia Suemi Mizuno, Raquel Alves Dos Santos
{"title":"p53 mediates the cellular responses to <i>cis</i>-trimethoxystilbene in breast cancer cell lines.","authors":"Julia Mirian Paulino, Natália Dos Santos Gonçalves, Heber Eduardo Andrada, Arthur Barcelos Ribeiro, Carlos Henrique Inácio Ramos, Cassia Suemi Mizuno, Raquel Alves Dos Santos","doi":"10.1080/14786419.2025.2566908","DOIUrl":"https://doi.org/10.1080/14786419.2025.2566908","url":null,"abstract":"<p><p>The present study investigated the cellular inhibitory effects of <i>cis</i>-trimethoxystilbene (<i>cis</i>-TMS) on the chemoresistant MDA-MB-231 breast cancer cell line and the role of p53 in mediating the cellular responses to this compound. Treatment with <i>cis</i>-TMS significantly inhibited the growth of MDA-MB-231 cells, reducing the cell viability by approximately 75% at 2.5 µM and increasing apoptosis rates to nearly 20%. Silencing p53 in MCF-10A and MCF-7 cell lines revealed that <i>cis-</i>TMS-induced apoptosis and cell cycle arrest were p53-dependent. The interaction studies suggest that <i>cis</i>-TMS does not alter the conformation of p53 at low concentrations but may influence its activity at higher doses. The results suggest that <i>cis</i>-TMS selectively targets cancer cells with functional p53, offering a potential targeted therapy approach. However, the reliance on p53 and the risk of genomic instability pose limitations, requiring further research to optimise the therapeutic strategies involving <i>cis</i>-TMS.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.6,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaoli Ma, Jingjing Tian, Ruru Sun, Wenhao Fan, Lei Wang, Hao Ma, Changcai Bai, Bin Ma, Jian Ma, Zhijun Song
{"title":"Chemical constituents from the whole herbs of <i>Vincetoxicum mongolicum</i> Maxim.","authors":"Xiaoli Ma, Jingjing Tian, Ruru Sun, Wenhao Fan, Lei Wang, Hao Ma, Changcai Bai, Bin Ma, Jian Ma, Zhijun Song","doi":"10.1080/14786419.2025.2566905","DOIUrl":"https://doi.org/10.1080/14786419.2025.2566905","url":null,"abstract":"<p><p>A new polyphenolic compound, vinmonone (<b>1</b>), together with 15 previously reported compounds (<b>2 </b>-<b> 16</b>) were isolated and characterised from the whole herbs of <i>Vincetoxicum mongolicum</i> Maxim. The structure of new compound (<b>1</b>) was determined through MR and HR-ESI-MS spectroscopic data analysis. Biological assay results showed that compounds <b>7 </b>-<b> 9</b>, <b>11</b> and <b>12</b> showed antioxidant activity with IC<sub>50</sub> 5.6, 11.8, 5.4, 6.2, 11.2 ug/mL, respectively, compounds <b>7</b>, <b>9</b> and <b>11</b> showed tyrosinase inhibitory activity with IC<sub>50</sub> 33.1, 30.1, 34.7 ug/mL, respectively. In addition, compounds <b>3</b> and <b>16</b> showed anti-bacterial activity against <i>Staphylococcus aureus</i> with MIC 25.1, 20.5 ug/mL, respectively, compounds <b>3</b> and <b>7</b> also showed anti-fungi activity against <i>Candida albicans</i> with MIC 3.4, 4.8 μg/mL, respectively. These finding provides a scientific basis for elucidating the chemical diversity and activity of <i>V. mongolicum.</i></p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.6,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145233046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}